Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0012171

Physical Location & Seq

Genomic Coordinates Forward (+)

7787907 .. 7790949

Gene Structure 3043 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016576

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGGTGGCCGCCAAGAAAAAAAGCGCGAAATCCACAGAAGCAGCAGAAGACGGTGAGATTTTCTCGGCTTCTACTTTCTCAAGTCTCGGCCTTCACTCCAATTTATGCGACCAGCTTCAAGAGAGGTTGGGCTTCAAGGGTCCGACTCTAGTCCAAGCCCAAGCGATCCCGGTTATTCTCTCCGGCCGCCACGTGCTGGTGAATGCGGCGACGGGGACGGGGAAAACGGTGGCGTATTTGGCTCCGGTTGTACACCGCCTGAGCTTGCAGCAGCCTCGGATTCAGCGCTCCGATGGTACTTTTGCTTTGGTTCTCGTGCCGACGAGAGAGCTGTGTCTGCAGGTTCATGAGATTTTGCACAAGTTACTGCACCGTTTTCACTGGATTGTGCCTGGTTATGTGATGGGAGGTGAAAAGAGGGAAAAGGAGAAGGCTAGATTGCGCAAAGGCATATCTATTCTCGTTGCAACTCCTGGAAGGCTGTTGGATCATTTGAAGCATACATCATCATTTTCACACACGAATTTGCGGTGGATAATTTTCGATGAAGCAGATAGGATTTTGGAACTTGGATTTGGAAAGGAGATAGAGGAAATACTAGATCTTTTGGGTTCGAGGAAACATGCCAAGGATAGTGGTGCGTCAAGTAAATTTGAATTTCAGAGGCAGAATTTGCTGTTGTCTGCGACCTTGAATGAAAAAGTCAATCATCTTGCAAAGATGAGCTTAGAGAAACCGGTCTTGATTGGTATGCCTGACAAGAAGATGCGGTCGATTACAAAATTTGAACCTTTAGGGTCTTCGGACTCTGACACAGGGGAACAGCAATATTCTGTGAAGGCAACAGGCTCTACAAATGAAGATTATAAACTTCCAGCCCAATTGACTCAGAGATATGTTAAAGTACCATGTAATGCGCGGCTTGCTGTACTTCTTTCCATTCTAAAGCATCTTTTTGAGCGAGAAACTTCTGAAAAAATTGTGCTATTCTTTTCAACATGTGATGCAGTGGATTTTCACTACTCTTTGTTAAGTGAGTTCCAATTCAAACCTAATTCAGGACCAGGATCAGAACTTAAGCAGAAATTTCTTAGATGCAAGACCTTCAGGTTACATGGCAATATGGAGCAAGTGGATCGAAGAACCACATTCGAGACCTTTAAAACAGAAAAATCAGCTCTTCTTTTGTCCACGGATGTGTCTGCCAGAGGTTTGGATTTCCCCAAAGTTAGATGTATCATACAGTATGATTCTCCAGGCGAGGCTACTGAATATGTGCATAGGGTTGGCAGAACAGCTCGGCTGGGTGAAAGAGGAGATTCAATATTATTTTTACAGCCAATTGAGATCGATTATTTGAAAGAATTGGAGAAGCATGGTGTTTCTCTAGTAGAGTATCCTCTCCTCAAAGTATTAGATAGTTTCCCACTATATGGTCAGCAGCATGTCAGGAAGTTTGTTTCCATTGATGCACATCCTTGGGTGCTGGCTTTGCAGAAGGCACTTGAAGCGTTTACAGATCAGCCAAACATAAAGCCTCTGGCCAAGAATGCATTTTGCTCTTGGGTTCGTGCGTACACTGCCCACCGTGGTGAGCTAAAGAGAATTTTTGTAGCAAAGAAACTTCACTTGGGACATGTTGCAAAAAGCTTTGCATTAAAAGAACAACCTTCCTTGGTTGGTGGTTCGTTTCACAAGCAAGCACAGAAGAGGAAGAGAGACGAAAGGCAGAAGGGAGGAATGAAAAAGAGGAAGTTTAACAGGAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

591

Amino Acids

66.64

Weight (kDa)

9.7

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	50 - 236	9.2e-42	DEAD/DEAH box helicase
ResIII	PF04851	52 - 199	3.1e-10	Type III restriction enzyme, res subunit
Helicase_C	PF00271	313 - 436	1.1e-22	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	509 - 558	3e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40700
fragaria_vesca	FvH4_1g09140 FvH4_1g09140 FvH4_1g09140
malus_domestica	MD02G1096800.v1.1
prunus_persica	Prupe.7G194100_v2.0.a1
pyrus_communis	pycom02g07680 pycom15g19610
rosa_chinensis	RchiOBHm_Chr2g0095531
rosa_laevigata	RLG00000016576
rosa_multiflora	Rmu_co8308315.1_g000001 Rmu_co8333539.1_g000001
rosa_roxburghii	Rroxscaffold_2G00146220
rosa_rugosa	Rorug02G0052200
rosa_samantha	Rh2AG100000 Rh2BG101400 Rh2CG103600 Rh2DG100900
rosa_wichuraiana	Rw2G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	867
Acc16I	TGCGCA	1 cut(s)	443
Acc36I	ACCTGC	1 cut(s)	331
AccB7I	CCANNNNNTGG	2 cut(s)	196, 1433
AccII	CGCG	2 cut(s)	26, 919
AciI	CCGC	7 cut(s)	9, 187, 206, 256, 529, 769, 919
AclWI	GGATC	4 cut(s)	160, 495, 1075, 1143
AcoI	YGGCCR	3 cut(s)	6, 184, 1542
AcsI	RAATTY	7 cut(s)	523, 650, 656, 670, 782, 1085, 1605
AcuI	CTGAAG	1 cut(s)	1090
AcvI	CACGTG	1 cut(s)	193
AfaI	GTAC	5 cut(s)	252, 298, 906, 930, 1577
AfeI	AGCGCT	1 cut(s)	287
AfiI	CCNNNNNNNGG	3 cut(s)	196, 1058, 1433
AflII	CTTAAG	1 cut(s)	1076
AflIII	ACRYGT	2 cut(s)	998, 1636
AgeI	ACCGGT	1 cut(s)	736
AjnI	CCWGG	4 cut(s)	391, 472, 1063, 1255
AjuI	GAANNNNNNNTTGG	4 cut(s)	558, 590, 1613, 1645
AleI	CACNNNNGTG	2 cut(s)	1196, 1590
AluBI	AGCT	8 cut(s)	115, 264, 331, 726, 1178, 1298, 1597, 1650
AluI	AGCT	8 cut(s)	115, 264, 331, 726, 1178, 1298, 1597, 1650
Alw26I	GTCTC	3 cut(s)	89, 1148, 1713
AlwI	GGATC	4 cut(s)	160, 495, 1075, 1143
Aor51HI	AGCGCT	1 cut(s)	287
AoxI	GGCC	4 cut(s)	6, 88, 184, 1542
ApeKI	GCWGC	4 cut(s)	41, 268, 271, 1441
ApoI	RAATTY	7 cut(s)	523, 650, 656, 670, 782, 1085, 1605
AsiGI	ACCGGT	1 cut(s)	736
AspLEI	GCGC	4 cut(s)	26, 288, 444, 919
AspS9I	GGNCC	2 cut(s)	140, 1061
AsuC2I	CCSGG	1 cut(s)	170
AsuHPI	GGTGA	5 cut(s)	65, 211, 422, 1319, 1604
AvaII	GGWCC	2 cut(s)	140, 1061
BalI	TGGCCA	1 cut(s)	1544
BauI	CACGAG	1 cut(s)	314
BbrPI	CACGTG	1 cut(s)	193
BbsI	GAAGAC	2 cut(s)	54, 792
BbvI	GCAGC	4 cut(s)	53, 280, 283, 1453
BccI	CCATC	2 cut(s)	287, 397
BcgI	CGANNNNNNTGC	4 cut(s)	257, 291, 1118, 1152
BciT130I	CCWGG	4 cut(s)	393, 474, 1065, 1257
BciVI	GTATCC	1 cut(s)	1407
BcnI	CCSGG	1 cut(s)	170
BcoDI	GTCTC	3 cut(s)	89, 1148, 1713
BfaI	CTAG	4 cut(s)	149, 435, 599, 1388
BfmI	CTRYAG	1 cut(s)	338
BfoI	RGCGCY	1 cut(s)	289
BfrI	CTTAAG	1 cut(s)	1076
BfuAI	ACCTGC	1 cut(s)	331
BfuI	GTATCC	1 cut(s)	1407
BglII	AGATCT	1 cut(s)	601
BisI	GCNGC	8 cut(s)	9, 42, 187, 207, 269, 272, 920, 1442
BlsI	GCNGC	8 cut(s)	10, 43, 188, 208, 270, 273, 921, 1443
Bme1390I	CCNGG	5 cut(s)	170, 393, 474, 1065, 1257
Bme18I	GGWCC	2 cut(s)	140, 1061
BmgT120I	GGNCC	2 cut(s)	140, 1061
BmiI	GGNNCC	2 cut(s)	141, 243
BmrFI	CCNGG	5 cut(s)	170, 393, 474, 1065, 1257
BmsI	GCATC	5 cut(s)	756, 958, 994, 1085, 1459
BoxI	GACNNNNGTC	1 cut(s)	149
BpiI	GAAGAC	2 cut(s)	54, 792
BplI	GAGNNNNNCTC	2 cut(s)	47, 79
BpmI	CTGGAG	1 cut(s)	1239
Bpu10I	CCTNAGC	1 cut(s)	260
BpuEI	CTTGAG	1 cut(s)	64
BpuMI	CCSGG	1 cut(s)	170
Bsa29I	ATCGAT	1 cut(s)	1350
BsaAI	YACGTR	1 cut(s)	193
BsaBI	GATNNNNATC	1 cut(s)	1473
BsaI	GGTCTC	1 cut(s)	1148
BsaJI	CCNNGG	6 cut(s)	275, 627, 1191, 1478, 1588, 1674
BsaWI	WCCGGW	2 cut(s)	244, 736
Bsc4I	CCNNNNNNNGG	3 cut(s)	196, 1058, 1433
Bse118I	RCCGGY	1 cut(s)	736
Bse1I	ACTGG	1 cut(s)	386
Bse8I	GATNNNNATC	1 cut(s)	1473
BseBI	CCWGG	4 cut(s)	393, 474, 1065, 1257
BseCI	ATCGAT	1 cut(s)	1350
BseDI	CCNNGG	6 cut(s)	275, 627, 1191, 1478, 1588, 1674
BseGI	GGATG	2 cut(s)	1201, 1474
BseJI	GATNNNNATC	1 cut(s)	1473
BseLI	CCNNNNNNNGG	3 cut(s)	196, 1058, 1433
BseMII	CTCAG	2 cut(s)	251, 902
BseNI	ACTGG	1 cut(s)	386
BseRI	GAGGAG	2 cut(s)	1329, 1394
BseX3I	CGGCCG	1 cut(s)	184
BseXI	GCAGC	4 cut(s)	53, 280, 283, 1453
BseYI	CCCAGC	1 cut(s)	1303
BsgI	GTGCAG	1 cut(s)	353
Bsh1236I	CGCG	2 cut(s)	26, 919
Bsh1285I	CGRYCG	2 cut(s)	187, 773
BshFI	GGCC	4 cut(s)	8, 90, 186, 1544
BshTI	ACCGGT	1 cut(s)	736
BshVI	ATCGAT	1 cut(s)	1350
BsiEI	CGRYCG	2 cut(s)	187, 773
BsiSI	CCGG	4 cut(s)	170, 183, 245, 737
BslFI	GGGAC	2 cut(s)	229, 1647
BslI	CCNNNNNNNGG	3 cut(s)	196, 1058, 1433
BsmAI	GTCTC	3 cut(s)	89, 1148, 1713
BsmBI	CGTCTC	1 cut(s)	1713
BsmFI	GGGAC	2 cut(s)	229, 1647
BsmI	GAATGC	2 cut(s)	208, 1555
BsnI	GGCC	4 cut(s)	8, 90, 186, 1544
Bso31I	GGTCTC	1 cut(s)	1148
Bsp1407I	TGTACA	1 cut(s)	250
Bsp143I	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
BspACI	CCGC	7 cut(s)	9, 187, 206, 256, 529, 769, 919
BspANI	GGCC	4 cut(s)	8, 90, 186, 1544
BspCNI	CTCAG	2 cut(s)	252, 901
BspDI	ATCGAT	1 cut(s)	1350
BspFNI	CGCG	2 cut(s)	26, 919
BspHI	TCATGA	1 cut(s)	346
BspLI	GGNNCC	2 cut(s)	141, 243
BspMAI	CTGCAG	1 cut(s)	342
BspMI	ACCTGC	1 cut(s)	331
BspPI	GGATC	4 cut(s)	160, 495, 1075, 1143
BspQI	GCTCTTC	1 cut(s)	1185
BspTI	CTTAAG	1 cut(s)	1076
BspTNI	GGTCTC	1 cut(s)	1148
BsrFI	RCCGGY	1 cut(s)	736
BsrGI	TGTACA	1 cut(s)	250
BsrI	ACTGG	1 cut(s)	386
BssAI	RCCGGY	1 cut(s)	736
BssECI	CCNNGG	6 cut(s)	275, 627, 1191, 1478, 1588, 1674
BssMI	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
BssSI	CACGAG	1 cut(s)	314
BssT1I	CCWWGG	3 cut(s)	627, 1478, 1674
Bst2BI	CACGAG	1 cut(s)	314
Bst2UI	CCWGG	4 cut(s)	393, 474, 1065, 1257
Bst4CI	ACNGT	5 cut(s)	53, 229, 374, 1245, 1589
Bst6I	CTCTTC	3 cut(s)	1185, 1703, 1709
BstAFI	CTTAAG	1 cut(s)	1076
BstAPI	GCANNNNNTGC	1 cut(s)	673
BstAUI	TGTACA	1 cut(s)	250
BstBAI	YACGTR	1 cut(s)	193
BstC8I	GCNNGC	4 cut(s)	266, 924, 1488, 1701
BstDEI	CTNAG	4 cut(s)	260, 727, 888, 1091
BstDSI	CCRYGG	2 cut(s)	1191, 1588
BstENI	CCTNNNNNAGG	1 cut(s)	1056
BstF5I	GGATG	2 cut(s)	1201, 1474
BstFNI	CGCG	2 cut(s)	26, 919
BstH2I	RGCGCY	1 cut(s)	289
BstHHI	GCGC	4 cut(s)	26, 288, 444, 919
BstKTI	GATC	7 cut(s)	168, 490, 604, 1070, 1138, 1350, 1522
BstMAI	GTCTC	3 cut(s)	89, 1148, 1713
BstMBI	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
BstMCI	CGRYCG	2 cut(s)	187, 773
BstMWI	GCNNNNNNNGC	8 cut(s)	239, 337, 673, 923, 1492, 1508, 1550, 1580
BstNI	CCWGG	4 cut(s)	393, 474, 1065, 1257
BstNSI	RCATGY	4 cut(s)	626, 1002, 1448, 1640
BstPAI	GACNNNNGTC	1 cut(s)	149
BstSCI	CCNGG	5 cut(s)	168, 391, 472, 1063, 1255
BstSFI	CTRYAG	1 cut(s)	338
BstUI	CGCG	2 cut(s)	26, 919
BstV1I	GCAGC	4 cut(s)	53, 280, 283, 1453
BstV2I	GAAGAC	2 cut(s)	54, 792
BstX2I	RGATCY	1 cut(s)	601
BstXI	CCANNNNNNTGG	1 cut(s)	1213
BstYI	RGATCY	1 cut(s)	601
BstZI	CGGCCG	1 cut(s)	184
Bsu15I	ATCGAT	1 cut(s)	1350
BsuI	GTATCC	1 cut(s)	1407
BsuRI	GGCC	4 cut(s)	8, 90, 186, 1544
BsuTUI	ATCGAT	1 cut(s)	1350
BtgI	CCRYGG	2 cut(s)	1191, 1588
BtsCI	GGATG	2 cut(s)	1201, 1474
BtsI	GCAGTG	2 cut(s)	1014, 1578
BtsIMutI	CAGTG	3 cut(s)	379, 1014, 1578
BveI	ACCTGC	1 cut(s)	331
Cac8I	GCNNGC	4 cut(s)	266, 924, 1488, 1701
CciI	TCATGA	1 cut(s)	346
CfoI	GCGC	4 cut(s)	26, 288, 444, 919
Cfr10I	RCCGGY	1 cut(s)	736
Cfr13I	GGNCC	2 cut(s)	140, 1061
ClaI	ATCGAT	1 cut(s)	1350
CseI	GACGC	1 cut(s)	630
Csp6I	GTAC	5 cut(s)	251, 297, 905, 929, 1576
CspAI	ACCGGT	1 cut(s)	736
CspCI	CAANNNNNGTGG	2 cut(s)	616, 651
CviAII	CATG	9 cut(s)	347, 623, 909, 999, 1115, 1376, 1445, 1637, 1770
CviQI	GTAC	5 cut(s)	251, 297, 905, 929, 1576
DdeI	CTNAG	4 cut(s)	260, 727, 888, 1091
DpnI	GATC	7 cut(s)	167, 489, 603, 1069, 1137, 1349, 1521
DpnII	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
DraI	TTTAAA	1 cut(s)	1162
EaeI	YGGCCR	3 cut(s)	6, 184, 1542
EagI	CGGCCG	1 cut(s)	184
Eam1104I	CTCTTC	3 cut(s)	1185, 1703, 1709
EarI	CTCTTC	3 cut(s)	1185, 1703, 1709
EclXI	CGGCCG	1 cut(s)	184
Eco130I	CCWWGG	3 cut(s)	627, 1478, 1674
Eco31I	GGTCTC	1 cut(s)	1148
Eco47I	GGWCC	2 cut(s)	140, 1061
Eco47III	AGCGCT	1 cut(s)	287
Eco52I	CGGCCG	1 cut(s)	184
Eco57I	CTGAAG	1 cut(s)	1090
Eco72I	CACGTG	1 cut(s)	193
EcoNI	CCTNNNNNAGG	1 cut(s)	1056
EcoRII	CCWGG	4 cut(s)	391, 472, 1063, 1255
EcoT14I	CCWWGG	3 cut(s)	627, 1478, 1674
EcoT22I	ATGCAT	1 cut(s)	1555
ErhI	CCWWGG	3 cut(s)	627, 1478, 1674
Esp3I	CGTCTC	1 cut(s)	1713
FaeI	CATG	9 cut(s)	350, 626, 912, 1002, 1118, 1379, 1448, 1640, 1773
FalI	AAGNNNNNCTT	2 cut(s)	710, 742
FaqI	GGGAC	2 cut(s)	229, 1647
FatI	CATG	9 cut(s)	346, 622, 908, 998, 1114, 1375, 1444, 1636, 1769
Fnu4HI	GCNGC	8 cut(s)	9, 42, 187, 207, 269, 272, 920, 1442
FokI	GGATG	2 cut(s)	1208, 1461
Fsp4HI	GCNGC	8 cut(s)	9, 42, 187, 207, 269, 272, 920, 1442
FspBI	CTAG	4 cut(s)	149, 435, 599, 1388
FspI	TGCGCA	1 cut(s)	443
GlaI	GCGC	4 cut(s)	25, 287, 443, 918
GluI	GCNGC	8 cut(s)	9, 42, 187, 207, 269, 272, 920, 1442
GsaI	CCCAGC	1 cut(s)	1307
GsuI	CTGGAG	1 cut(s)	1239
HaeII	RGCGCY	1 cut(s)	289
HaeIII	GGCC	4 cut(s)	8, 90, 186, 1544
HapII	CCGG	4 cut(s)	170, 183, 245, 737
HgaI	GACGC	1 cut(s)	630
HhaI	GCGC	4 cut(s)	26, 288, 444, 919
Hin1II	CATG	9 cut(s)	350, 626, 912, 1002, 1118, 1379, 1448, 1640, 1773
Hin6I	GCGC	4 cut(s)	24, 286, 442, 917
HinP1I	GCGC	4 cut(s)	24, 286, 442, 917
HindIII	AAGCTT	1 cut(s)	1648
HinfI	GANTC	6 cut(s)	145, 280, 806, 886, 1250, 1319
HpaII	CCGG	4 cut(s)	170, 183, 245, 737
HphI	GGTGA	5 cut(s)	65, 211, 422, 1319, 1604
Hpy166II	GTNNAC	4 cut(s)	253, 1191, 1515, 1578
Hpy188I	TCNGA	9 cut(s)	144, 279, 292, 663, 805, 811, 891, 973, 1072
Hpy188III	TCNNGA	6 cut(s)	119, 347, 742, 1059, 1153, 1450
Hpy8I	GTNNAC	4 cut(s)	253, 1191, 1515, 1578
Hpy99I	CGWCG	2 cut(s)	214, 325
HpyAV	CCTTC	8 cut(s)	101, 424, 471, 832, 1114, 1492, 1680, 1726
HpyCH4III	ACNGT	5 cut(s)	53, 229, 374, 1245, 1589
HpyCH4IV	ACGT	1 cut(s)	192
HpyF10VI	GCNNNNNNNGC	8 cut(s)	239, 337, 673, 923, 1492, 1508, 1550, 1580
HpyF3I	CTNAG	4 cut(s)	260, 727, 888, 1091
HpySE526I	ACGT	1 cut(s)	192
Hsp92II	CATG	9 cut(s)	350, 626, 912, 1002, 1118, 1379, 1448, 1640, 1773
HspAI	GCGC	4 cut(s)	24, 286, 442, 917
Kzo9I	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
LguI	GCTCTTC	1 cut(s)	1185
LmnI	GCTCC	3 cut(s)	247, 293, 1126
Lsp1109I	GCAGC	4 cut(s)	53, 280, 283, 1453
LweI	GCATC	5 cut(s)	756, 958, 994, 1085, 1459
MaeI	CTAG	4 cut(s)	149, 435, 599, 1388
MaeII	ACGT	1 cut(s)	192
MaeIII	GTNAC	2 cut(s)	363, 1110
MalI	GATC	7 cut(s)	167, 489, 603, 1069, 1137, 1349, 1521
MboI	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
MboII	GAAGA	8 cut(s)	59, 775, 792, 872, 1152, 1172, 1720, 1726
MfeI	CAATTG	2 cut(s)	881, 1341
MflI	RGATCY	1 cut(s)	601
MlsI	TGGCCA	1 cut(s)	1544
MluNI	TGGCCA	1 cut(s)	1544
MlyI	GAGTC	3 cut(s)	139, 800, 880
MmeI	TCCRAC	2 cut(s)	167, 465
Mox20I	TGGCCA	1 cut(s)	1544
Mph1103I	ATGCAT	1 cut(s)	1555
MscI	TGGCCA	1 cut(s)	1544
MseI	TTAA	6 cut(s)	900, 1031, 1077, 1161, 1658, 1758
MslI	CAYNNNNRTG	2 cut(s)	1196, 1590
Msp20I	TGGCCA	1 cut(s)	1544
MspCI	CTTAAG	1 cut(s)	1076
MspI	CCGG	4 cut(s)	170, 183, 245, 737
MspR9I	CCNGG	5 cut(s)	170, 393, 474, 1065, 1257
MunI	CAATTG	2 cut(s)	881, 1341
Mva1269I	GAATGC	2 cut(s)	208, 1555
MvaI	CCWGG	4 cut(s)	393, 474, 1065, 1257
MvnI	CGCG	2 cut(s)	26, 919
MwoI	GCNNNNNNNGC	8 cut(s)	239, 337, 673, 923, 1492, 1508, 1550, 1580
NciI	CCSGG	1 cut(s)	170
NdeII	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
NlaIII	CATG	9 cut(s)	350, 626, 912, 1002, 1118, 1379, 1448, 1640, 1773
NlaIV	GGNNCC	2 cut(s)	141, 243
NmeAIII	GCCGAG	3 cut(s)	44, 66, 1279
NsbI	TGCGCA	1 cut(s)	443
NsiI	ATGCAT	1 cut(s)	1555
NspI	RCATGY	4 cut(s)	626, 1002, 1448, 1640
OliI	CACNNNNGTG	2 cut(s)	1196, 1590
PagI	TCATGA	1 cut(s)	346
PciI	ACATGT	2 cut(s)	998, 1636
PciSI	GCTCTTC	1 cut(s)	1185
PctI	GAATGC	2 cut(s)	208, 1555
PfeI	GAWTC	3 cut(s)	280, 1250, 1319
PflMI	CCANNNNNTGG	2 cut(s)	196, 1433
PfoI	TCCNGGA	1 cut(s)	472
PinAI	ACCGGT	1 cut(s)	736
PkrI	GCNGC	8 cut(s)	10, 43, 188, 208, 270, 273, 921, 1443
PleI	GAGTC	3 cut(s)	139, 800, 880
PmaCI	CACGTG	1 cut(s)	193
PmlI	CACGTG	1 cut(s)	193
PpsI	GAGTC	3 cut(s)	139, 800, 880
Ppu21I	YACGTR	1 cut(s)	193
PscI	ACATGT	2 cut(s)	998, 1636
PshAI	GACNNNNGTC	1 cut(s)	149
PsiI	TTATAA	1 cut(s)	867
Psp6I	CCWGG	4 cut(s)	391, 472, 1063, 1255
PspCI	CACGTG	1 cut(s)	193
PspFI	CCCAGC	1 cut(s)	1303
PspGI	CCWGG	4 cut(s)	391, 472, 1063, 1255
PspN4I	GGNNCC	2 cut(s)	141, 243
PspPI	GGNCC	2 cut(s)	140, 1061
PstI	CTGCAG	1 cut(s)	342
PsuI	RGATCY	1 cut(s)	601
RsaI	GTAC	5 cut(s)	252, 298, 906, 930, 1577
RsaNI	GTAC	5 cut(s)	251, 297, 905, 929, 1576
RseI	CAYNNNNRTG	2 cut(s)	1196, 1590
SapI	GCTCTTC	1 cut(s)	1185
SaqAI	TTAA	6 cut(s)	900, 1031, 1077, 1161, 1658, 1758
SatI	GCNGC	8 cut(s)	9, 42, 187, 207, 269, 272, 920, 1442
Sau3AI	GATC	7 cut(s)	165, 487, 601, 1067, 1135, 1347, 1519
Sau96I	GGNCC	2 cut(s)	140, 1061
SchI	GAGTC	3 cut(s)	139, 800, 880
ScrFI	CCNGG	5 cut(s)	170, 393, 474, 1065, 1257
SfaNI	GCATC	5 cut(s)	756, 958, 994, 1085, 1459
SfcI	CTRYAG	1 cut(s)	338
SinI	GGWCC	2 cut(s)	140, 1061
SmiMI	CAYNNNNRTG	2 cut(s)	1196, 1590
SmlI	CTYRAG	2 cut(s)	79, 1076
SmoI	CTYRAG	2 cut(s)	79, 1076
SsiI	CCGC	7 cut(s)	9, 187, 206, 256, 529, 769, 919
SspI	AATATT	2 cut(s)	830, 1326
SspMI	CTAG	4 cut(s)	149, 435, 599, 1388
StyD4I	CCNGG	5 cut(s)	168, 391, 472, 1063, 1255
StyI	CCWWGG	3 cut(s)	627, 1478, 1674
TaaI	ACNGT	5 cut(s)	53, 229, 374, 1245, 1589
TaiI	ACGT	1 cut(s)	195
TaqI	TCGA	6 cut(s)	543, 614, 773, 1138, 1152, 1350
TatI	WGTACW	2 cut(s)	250, 928
TauI	GCSGC	4 cut(s)	11, 189, 209, 922
TfiI	GAWTC	3 cut(s)	280, 1250, 1319
Tru1I	TTAA	6 cut(s)	900, 1031, 1077, 1161, 1658, 1758
Tru9I	TTAA	6 cut(s)	900, 1031, 1077, 1161, 1658, 1758
TscAI	CASTG	3 cut(s)	386, 1014, 1585
TseI	GCWGC	4 cut(s)	41, 268, 271, 1441
TspDTI	ATGAA	5 cut(s)	335, 561, 711, 873, 1757
TspGWI	ACGGA	1 cut(s)	1208
TspRI	CASTG	3 cut(s)	386, 1014, 1585
Van91I	CCANNNNNTGG	2 cut(s)	196, 1433
Vha464I	CTTAAG	1 cut(s)	1076
VpaK11BI	GGWCC	2 cut(s)	140, 1061
XagI	CCTNNNNNAGG	1 cut(s)	1056
XapI	RAATTY	7 cut(s)	523, 650, 656, 670, 782, 1085, 1605
XceI	RCATGY	4 cut(s)	626, 1002, 1448, 1640
XspI	CTAG	4 cut(s)	149, 435, 599, 1388
Zsp2I	ATGCAT	1 cut(s)	1555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.