Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0012171

Physical Location & Seq

Genomic Coordinates Reverse (-)

84165917 .. 84169414

Gene Structure 3498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00146220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGTGGCCGCCAAGAAAAAAAGCGCGAAATCCACAGAAGACGGTGAGATTTTCTCGGCTTCTACTTTCTCAAGTCTCGGCCTTCACTCCAATTTATGCGACCAGCTTCAAGAGAGGTTGGGCTTCAAAGGTCCGACGCTAGTTCAAGCCCAAGCGATTCCTGTAATTCTGTCCGGCCGCCACGTGCTGGTGAATGCGGCGACGGGGACGGGGAAAACGGTGGCGTATTTGGCTCCGGTTGTACACCACCTGAGCTTGCAGCAGCCTCGGATTCAGCGCTCCGATGGTACTTTTGCTTTGGTTCTCGTGCCGACGAGAGAGCTGTGTCTGCAGGTTCATGAGATTTTGCACAAGTTACTGCACCGTTTTCACTGGATTGTGCCTGGTTATGTGATGGGAGGTGAAAAGAGGGAAAAGGAGAAGGCTAGATTGCGCAAAGGCATATCTATTCTTGTTGCAACGCCTGGAAGGCTGTTGGATCATTTGAAGCGTACATCATCATTTTCGCACACGAATTTGCGGTGGATAATTTTCGATGAAGCAGATAGGATTTTGGAACTTGGATTTGGAAAGGAGATAGAGGAAATACTAGATCTTTTGGGTTCGAGGAAACATGCCAAGGATAGTGGTGCGTCAAGTAAATTTGAATTTCAGAGGCAGAATTTGCTGTTGTCTGCGACCTTGAATGAAAAAGTCAATCATCTTGCAAAGATGAGCTTAGACAAACCGGTCTTGATTGGTATGCCTGACAAGAAGATGCGGTCGATTACAAAATTTGAACCTTTAGGGTCTTCGGACTCTGACACAGAGGAACCGCAATATTCTGTGAAGGCCACAGGCTCTACAAATGAAGATTATAAACTTCCAGCCCAATTGACTCAGAGATATGTTAAAGTACCATGTAATGCGCGGCTTGCTGTACTTCTTTCCATTCTAAAGCATCTTTTTGAGCGAGAAACTTCTGAAAAAATTGTGCTATTCTTTTCAACATGTGATGCAGTGGATTTTCACTACTCTTTGTTAAGTGAGTTCCAATTCAAACCTAATTCAGGACCAGGATCAGAACTTAAGCAGAAATTTCTTAGATGCAAGACCTTCAGGTTGCATGGCAATATGGAACAAGTGGATCGAAGAACCACATTCGAAACCTTTAAAACAGAAAAATCAGCTCTTCTTTTGTCCACAGATGTTTCTGCCAGAGGTTTGGATTTCCCCAAAGTTAGATGTATCATACAGTATGATTCTCCAGGCGAGGCTACTGAATATGTGCATAGGGTTGGCAGAACAGCTCGGCTGGGTGAAAGAGGAGATTCAATATTATTTTTACAGCCAATTGAAATCGATTATTTGAAAGAATTGGAGAAGCATGGTGTTTCTCTAGTAGAGTATCCACTCCTCAAAGTAATAGATAGTTTCCCACTATATGGTCAGCAGCATGTCAGGAAGTTTGTTTCCATTGATGCACATCCTTGGGTGCTGGCTTTGCAAAAGGCACTTGAAGCGTTTACAGATCAGCCAAACATAAAGCCTCTGGCCAAGAATGCATTTTGCTCATGGGTTCGTGCATACACTGCCCACCGTGGTGAGCTGAAGAGAATTTTTGTGGCAAAGAAACTTCACTTGGGACATGTTGCAAAAAGCTTTGCATTAAAAGAACAACCTTCCTTGGTTGGGGGTTCGTTTCACAAGCAAGCACAGAAGAGGAAGAGAGACGAAAGGCAGAAGGGAGGAATGAAAAAGAAGAAGTTTAACAGGAAAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

587

Amino Acids

66.37

Weight (kDa)

9.69

Isoelectric Point (pI)

39.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	47 - 233	5.1e-42	DEAD/DEAH box helicase
ResIII	PF04851	49 - 196	7.2e-10	Type III restriction enzyme, res subunit
Helicase_C	PF00271	310 - 433	1.1e-22	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	506 - 555	3e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40700
fragaria_vesca	FvH4_1g09140 FvH4_1g09140 FvH4_1g09140
malus_domestica	MD02G1096800.v1.1
prunus_persica	Prupe.7G194100_v2.0.a1
pyrus_communis	pycom02g07680 pycom15g19610
rosa_chinensis	RchiOBHm_Chr2g0095531
rosa_laevigata	RLG00000016576
rosa_multiflora	Rmu_co8308315.1_g000001 Rmu_co8333539.1_g000001
rosa_roxburghii	Rroxscaffold_2G00146220
rosa_rugosa	Rorug02G0052200
rosa_samantha	Rh2AG100000 Rh2BG101400 Rh2CG103600 Rh2DG100900
rosa_wichuraiana	Rw2G007590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	858
AasI	GACNNNNNNGTC	1 cut(s)	728
Acc16I	TGCGCA	1 cut(s)	434
Acc36I	ACCTGC	1 cut(s)	322
AccB7I	CCANNNNNTGG	2 cut(s)	187, 1424
AccII	CGCG	2 cut(s)	26, 910
AciI	CCGC	7 cut(s)	9, 178, 197, 520, 760, 815, 910
AclWI	GGATC	3 cut(s)	486, 1066, 1134
AcoI	YGGCCR	3 cut(s)	6, 175, 1533
AcsI	RAATTY	7 cut(s)	514, 641, 647, 661, 773, 1076, 1596
AcuI	CTGAAG	2 cut(s)	1081, 1610
AcvI	CACGTG	1 cut(s)	184
AfaI	GTAC	5 cut(s)	243, 289, 493, 897, 921
AfeI	AGCGCT	1 cut(s)	278
AfiI	CCNNNNNNNGG	4 cut(s)	187, 1049, 1424, 1671
AflII	CTTAAG	1 cut(s)	1067
AflIII	ACRYGT	2 cut(s)	989, 1627
AgeI	ACCGGT	1 cut(s)	727
AjnI	CCWGG	4 cut(s)	382, 463, 1054, 1246
AjuI	GAANNNNNNNTTGG	4 cut(s)	549, 581, 1604, 1636
AleI	CACNNNNGTG	1 cut(s)	1581
AluBI	AGCT	8 cut(s)	106, 255, 322, 717, 1169, 1289, 1588, 1641
AluI	AGCT	8 cut(s)	106, 255, 322, 717, 1169, 1289, 1588, 1641
Alw26I	GTCTC	2 cut(s)	80, 1704
AlwI	GGATC	3 cut(s)	486, 1066, 1134
Aor51HI	AGCGCT	1 cut(s)	278
AoxI	GGCC	5 cut(s)	6, 79, 175, 831, 1533
ApeKI	GCWGC	3 cut(s)	259, 262, 1432
ApoI	RAATTY	7 cut(s)	514, 641, 647, 661, 773, 1076, 1596
AsiGI	ACCGGT	1 cut(s)	727
AspLEI	GCGC	4 cut(s)	26, 279, 435, 910
AspS9I	GGNCC	2 cut(s)	131, 1052
AsuHPI	GGTGA	5 cut(s)	56, 202, 413, 1310, 1595
AsuII	TTCGAA	1 cut(s)	1143
AvaII	GGWCC	2 cut(s)	131, 1052
BalI	TGGCCA	1 cut(s)	1535
BauI	CACGAG	1 cut(s)	305
BbrPI	CACGTG	1 cut(s)	184
BbsI	GAAGAC	2 cut(s)	45, 783
BbvI	GCAGC	3 cut(s)	271, 274, 1444
BccI	CCATC	2 cut(s)	278, 388
BcgI	CGANNNNNNTGC	2 cut(s)	248, 282
BciT130I	CCWGG	4 cut(s)	384, 465, 1056, 1248
BciVI	GTATCC	1 cut(s)	1398
BcoDI	GTCTC	2 cut(s)	80, 1704
BfaI	CTAG	4 cut(s)	140, 426, 590, 1379
BfmI	CTRYAG	1 cut(s)	329
BfoI	RGCGCY	1 cut(s)	280
BfrI	CTTAAG	1 cut(s)	1067
BfuAI	ACCTGC	1 cut(s)	322
BfuI	GTATCC	1 cut(s)	1398
BglI	GCCNNNNNGGC	1 cut(s)	469
BglII	AGATCT	1 cut(s)	592
BisI	GCNGC	7 cut(s)	9, 178, 198, 260, 263, 911, 1433
BlsI	GCNGC	7 cut(s)	10, 179, 199, 261, 264, 912, 1434
Bme1390I	CCNGG	4 cut(s)	384, 465, 1056, 1248
Bme18I	GGWCC	2 cut(s)	131, 1052
BmgT120I	GGNCC	2 cut(s)	131, 1052
BmiI	GGNNCC	2 cut(s)	234, 813
BmrFI	CCNGG	4 cut(s)	384, 465, 1056, 1248
BmsI	GCATC	5 cut(s)	747, 949, 985, 1076, 1450
BpiI	GAAGAC	2 cut(s)	45, 783
BplI	GAGNNNNNCTC	2 cut(s)	38, 70
BpmI	CTGGAG	1 cut(s)	1230
Bpu10I	CCTNAGC	1 cut(s)	251
Bpu14I	TTCGAA	1 cut(s)	1143
BpuEI	CTTGAG	1 cut(s)	55
Bsa29I	ATCGAT	1 cut(s)	1341
BsaAI	YACGTR	1 cut(s)	184
BsaBI	GATNNNNATC	1 cut(s)	1464
BsaJI	CCNNGG	5 cut(s)	266, 618, 1469, 1579, 1665
BsaWI	WCCGGW	2 cut(s)	235, 727
Bsc4I	CCNNNNNNNGG	4 cut(s)	187, 1049, 1424, 1671
Bse118I	RCCGGY	1 cut(s)	727
Bse1I	ACTGG	1 cut(s)	377
Bse8I	GATNNNNATC	1 cut(s)	1464
BseBI	CCWGG	4 cut(s)	384, 465, 1056, 1248
BseCI	ATCGAT	1 cut(s)	1341
BseDI	CCNNGG	5 cut(s)	266, 618, 1469, 1579, 1665
BseGI	GGATG	1 cut(s)	1465
BseJI	GATNNNNATC	1 cut(s)	1464
BseLI	CCNNNNNNNGG	4 cut(s)	187, 1049, 1424, 1671
BseMII	CTCAG	2 cut(s)	242, 893
BseNI	ACTGG	1 cut(s)	377
BseRI	GAGGAG	2 cut(s)	1320, 1385
BseX3I	CGGCCG	1 cut(s)	175
BseXI	GCAGC	3 cut(s)	271, 274, 1444
BseYI	CCCAGC	1 cut(s)	1294
BsgI	GTGCAG	1 cut(s)	344
Bsh1236I	CGCG	2 cut(s)	26, 910
Bsh1285I	CGRYCG	2 cut(s)	178, 764
BshFI	GGCC	5 cut(s)	8, 81, 177, 833, 1535
BshTI	ACCGGT	1 cut(s)	727
BshVI	ATCGAT	1 cut(s)	1341
BsiEI	CGRYCG	2 cut(s)	178, 764
BsiSI	CCGG	3 cut(s)	174, 236, 728
BslFI	GGGAC	2 cut(s)	220, 1638
BslI	CCNNNNNNNGG	4 cut(s)	187, 1049, 1424, 1671
BsmAI	GTCTC	2 cut(s)	80, 1704
BsmBI	CGTCTC	1 cut(s)	1704
BsmFI	GGGAC	2 cut(s)	220, 1638
BsmI	GAATGC	2 cut(s)	199, 1546
BsnI	GGCC	5 cut(s)	8, 81, 177, 833, 1535
Bsp119I	TTCGAA	1 cut(s)	1143
Bsp1407I	TGTACA	1 cut(s)	241
Bsp143I	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
BspACI	CCGC	7 cut(s)	9, 178, 197, 520, 760, 815, 910
BspANI	GGCC	5 cut(s)	8, 81, 177, 833, 1535
BspCNI	CTCAG	2 cut(s)	243, 892
BspDI	ATCGAT	1 cut(s)	1341
BspFNI	CGCG	2 cut(s)	26, 910
BspHI	TCATGA	1 cut(s)	337
BspLI	GGNNCC	2 cut(s)	234, 813
BspMAI	CTGCAG	1 cut(s)	333
BspMI	ACCTGC	1 cut(s)	322
BspPI	GGATC	3 cut(s)	486, 1066, 1134
BspQI	GCTCTTC	1 cut(s)	1176
BspT104I	TTCGAA	1 cut(s)	1143
BspTI	CTTAAG	1 cut(s)	1067
BsrFI	RCCGGY	1 cut(s)	727
BsrGI	TGTACA	1 cut(s)	241
BsrI	ACTGG	1 cut(s)	377
BssAI	RCCGGY	1 cut(s)	727
BssECI	CCNNGG	5 cut(s)	266, 618, 1469, 1579, 1665
BssMI	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
BssSI	CACGAG	1 cut(s)	305
BssT1I	CCWWGG	3 cut(s)	618, 1469, 1665
Bst2BI	CACGAG	1 cut(s)	305
Bst2UI	CCWGG	4 cut(s)	384, 465, 1056, 1248
Bst4CI	ACNGT	5 cut(s)	44, 220, 365, 1236, 1580
Bst6I	CTCTTC	4 cut(s)	1176, 1586, 1694, 1700
BstAFI	CTTAAG	1 cut(s)	1067
BstAPI	GCANNNNNTGC	2 cut(s)	664, 1571
BstAUI	TGTACA	1 cut(s)	241
BstBAI	YACGTR	1 cut(s)	184
BstBI	TTCGAA	1 cut(s)	1143
BstC8I	GCNNGC	4 cut(s)	257, 915, 1479, 1692
BstDEI	CTNAG	4 cut(s)	251, 718, 879, 1082
BstDSI	CCRYGG	1 cut(s)	1579
BstENI	CCTNNNNNAGG	1 cut(s)	1047
BstF5I	GGATG	1 cut(s)	1465
BstFNI	CGCG	2 cut(s)	26, 910
BstH2I	RGCGCY	1 cut(s)	280
BstHHI	GCGC	4 cut(s)	26, 279, 435, 910
BstKTI	GATC	5 cut(s)	481, 595, 1061, 1129, 1513
BstMAI	GTCTC	2 cut(s)	80, 1704
BstMBI	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
BstMCI	CGRYCG	2 cut(s)	178, 764
BstMWI	GCNNNNNNNGC	9 cut(s)	230, 328, 469, 664, 914, 1483, 1499, 1541, 1571
BstNI	CCWGG	4 cut(s)	384, 465, 1056, 1248
BstNSI	RCATGY	4 cut(s)	617, 993, 1439, 1631
BstSCI	CCNGG	4 cut(s)	382, 463, 1054, 1246
BstSFI	CTRYAG	1 cut(s)	329
BstUI	CGCG	2 cut(s)	26, 910
BstV1I	GCAGC	3 cut(s)	271, 274, 1444
BstV2I	GAAGAC	2 cut(s)	45, 783
BstX2I	RGATCY	1 cut(s)	592
BstXI	CCANNNNNNTGG	1 cut(s)	1204
BstYI	RGATCY	1 cut(s)	592
BstZI	CGGCCG	1 cut(s)	175
Bsu15I	ATCGAT	1 cut(s)	1341
BsuI	GTATCC	1 cut(s)	1398
BsuRI	GGCC	5 cut(s)	8, 81, 177, 833, 1535
BsuTUI	ATCGAT	1 cut(s)	1341
BtgI	CCRYGG	1 cut(s)	1579
BtsCI	GGATG	1 cut(s)	1465
BtsI	GCAGTG	2 cut(s)	1005, 1569
BtsIMutI	CAGTG	3 cut(s)	370, 1005, 1569
BveI	ACCTGC	1 cut(s)	322
Cac8I	GCNNGC	4 cut(s)	257, 915, 1479, 1692
CciI	TCATGA	1 cut(s)	337
CfoI	GCGC	4 cut(s)	26, 279, 435, 910
Cfr10I	RCCGGY	1 cut(s)	727
Cfr13I	GGNCC	2 cut(s)	131, 1052
ClaI	ATCGAT	1 cut(s)	1341
CseI	GACGC	2 cut(s)	145, 621
Csp6I	GTAC	5 cut(s)	242, 288, 492, 896, 920
CspAI	ACCGGT	1 cut(s)	727
CspCI	CAANNNNNGTGG	2 cut(s)	607, 642
CviQI	GTAC	5 cut(s)	242, 288, 492, 896, 920
DdeI	CTNAG	4 cut(s)	251, 718, 879, 1082
DpnI	GATC	5 cut(s)	480, 594, 1060, 1128, 1512
DpnII	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
DraI	TTTAAA	1 cut(s)	1153
DrdI	GACNNNNNNGTC	1 cut(s)	728
DseDI	GACNNNNNNGTC	1 cut(s)	728
EaeI	YGGCCR	3 cut(s)	6, 175, 1533
EagI	CGGCCG	1 cut(s)	175
Eam1104I	CTCTTC	4 cut(s)	1176, 1586, 1694, 1700
EarI	CTCTTC	4 cut(s)	1176, 1586, 1694, 1700
EclXI	CGGCCG	1 cut(s)	175
Eco130I	CCWWGG	3 cut(s)	618, 1469, 1665
Eco47I	GGWCC	2 cut(s)	131, 1052
Eco47III	AGCGCT	1 cut(s)	278
Eco52I	CGGCCG	1 cut(s)	175
Eco57I	CTGAAG	2 cut(s)	1081, 1610
Eco72I	CACGTG	1 cut(s)	184
EcoNI	CCTNNNNNAGG	1 cut(s)	1047
EcoRII	CCWGG	4 cut(s)	382, 463, 1054, 1246
EcoT14I	CCWWGG	3 cut(s)	618, 1469, 1665
EcoT22I	ATGCAT	1 cut(s)	1546
ErhI	CCWWGG	3 cut(s)	618, 1469, 1665
Esp3I	CGTCTC	1 cut(s)	1704
FalI	AAGNNNNNCTT	2 cut(s)	701, 733
FaqI	GGGAC	2 cut(s)	220, 1638
Fnu4HI	GCNGC	7 cut(s)	9, 178, 198, 260, 263, 911, 1433
FokI	GGATG	1 cut(s)	1452
Fsp4HI	GCNGC	7 cut(s)	9, 178, 198, 260, 263, 911, 1433
FspBI	CTAG	4 cut(s)	140, 426, 590, 1379
FspI	TGCGCA	1 cut(s)	434
GlaI	GCGC	4 cut(s)	25, 278, 434, 909
GluI	GCNGC	7 cut(s)	9, 178, 198, 260, 263, 911, 1433
GsaI	CCCAGC	1 cut(s)	1298
GsuI	CTGGAG	1 cut(s)	1230
HaeII	RGCGCY	1 cut(s)	280
HaeIII	GGCC	5 cut(s)	8, 81, 177, 833, 1535
HapII	CCGG	3 cut(s)	174, 236, 728
HgaI	GACGC	2 cut(s)	145, 621
HhaI	GCGC	4 cut(s)	26, 279, 435, 910
Hin6I	GCGC	4 cut(s)	24, 277, 433, 908
HinP1I	GCGC	4 cut(s)	24, 277, 433, 908
HindIII	AAGCTT	1 cut(s)	1639
HinfI	GANTC	6 cut(s)	157, 271, 797, 877, 1241, 1310
HpaII	CCGG	3 cut(s)	174, 236, 728
HphI	GGTGA	5 cut(s)	56, 202, 413, 1310, 1595
Hpy166II	GTNNAC	3 cut(s)	244, 1182, 1506
Hpy188I	TCNGA	9 cut(s)	135, 270, 283, 654, 796, 802, 882, 964, 1063
Hpy188III	TCNNGA	5 cut(s)	110, 338, 733, 1050, 1441
Hpy8I	GTNNAC	3 cut(s)	244, 1182, 1506
Hpy99I	CGWCG	3 cut(s)	139, 205, 316
HpyAV	CCTTC	7 cut(s)	92, 415, 462, 823, 1105, 1671, 1717
HpyCH4III	ACNGT	5 cut(s)	44, 220, 365, 1236, 1580
HpyCH4IV	ACGT	1 cut(s)	183
HpyF10VI	GCNNNNNNNGC	9 cut(s)	230, 328, 469, 664, 914, 1483, 1499, 1541, 1571
HpyF3I	CTNAG	4 cut(s)	251, 718, 879, 1082
HpySE526I	ACGT	1 cut(s)	183
HspAI	GCGC	4 cut(s)	24, 277, 433, 908
Kzo9I	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
LguI	GCTCTTC	1 cut(s)	1176
LmnI	GCTCC	2 cut(s)	238, 284
Lsp1109I	GCAGC	3 cut(s)	271, 274, 1444
LweI	GCATC	5 cut(s)	747, 949, 985, 1076, 1450
MaeI	CTAG	4 cut(s)	140, 426, 590, 1379
MaeII	ACGT	1 cut(s)	183
MaeIII	GTNAC	1 cut(s)	354
MalI	GATC	5 cut(s)	480, 594, 1060, 1128, 1512
MboI	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
MfeI	CAATTG	2 cut(s)	872, 1332
MflI	RGATCY	1 cut(s)	592
MlsI	TGGCCA	1 cut(s)	1535
MluNI	TGGCCA	1 cut(s)	1535
MlyI	GAGTC	2 cut(s)	791, 871
MmeI	TCCRAC	2 cut(s)	158, 456
Mox20I	TGGCCA	1 cut(s)	1535
Mph1103I	ATGCAT	1 cut(s)	1546
MscI	TGGCCA	1 cut(s)	1535
MseI	TTAA	6 cut(s)	891, 1022, 1068, 1152, 1649, 1749
MslI	CAYNNNNRTG	1 cut(s)	1581
Msp20I	TGGCCA	1 cut(s)	1535
MspCI	CTTAAG	1 cut(s)	1067
MspI	CCGG	3 cut(s)	174, 236, 728
MspR9I	CCNGG	4 cut(s)	384, 465, 1056, 1248
MunI	CAATTG	2 cut(s)	872, 1332
Mva1269I	GAATGC	2 cut(s)	199, 1546
MvaI	CCWGG	4 cut(s)	384, 465, 1056, 1248
MvnI	CGCG	2 cut(s)	26, 910
MwoI	GCNNNNNNNGC	9 cut(s)	230, 328, 469, 664, 914, 1483, 1499, 1541, 1571
NdeII	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
NlaIV	GGNNCC	2 cut(s)	234, 813
NmeAIII	GCCGAG	3 cut(s)	35, 57, 1270
NsbI	TGCGCA	1 cut(s)	434
NsiI	ATGCAT	1 cut(s)	1546
NspI	RCATGY	4 cut(s)	617, 993, 1439, 1631
NspV	TTCGAA	1 cut(s)	1143
OliI	CACNNNNGTG	1 cut(s)	1581
PagI	TCATGA	1 cut(s)	337
PciI	ACATGT	2 cut(s)	989, 1627
PciSI	GCTCTTC	1 cut(s)	1176
PctI	GAATGC	2 cut(s)	199, 1546
PfeI	GAWTC	4 cut(s)	157, 271, 1241, 1310
PflMI	CCANNNNNTGG	2 cut(s)	187, 1424
PinAI	ACCGGT	1 cut(s)	727
PkrI	GCNGC	7 cut(s)	10, 179, 199, 261, 264, 912, 1434
PleI	GAGTC	2 cut(s)	791, 871
PmaCI	CACGTG	1 cut(s)	184
PmlI	CACGTG	1 cut(s)	184
PpsI	GAGTC	2 cut(s)	791, 871
Ppu21I	YACGTR	1 cut(s)	184
PscI	ACATGT	2 cut(s)	989, 1627
PsiI	TTATAA	1 cut(s)	858
Psp6I	CCWGG	4 cut(s)	382, 463, 1054, 1246
PspCI	CACGTG	1 cut(s)	184
PspFI	CCCAGC	1 cut(s)	1294
PspGI	CCWGG	4 cut(s)	382, 463, 1054, 1246
PspN4I	GGNNCC	2 cut(s)	234, 813
PspPI	GGNCC	2 cut(s)	131, 1052
PstI	CTGCAG	1 cut(s)	333
PsuI	RGATCY	1 cut(s)	592
RsaI	GTAC	5 cut(s)	243, 289, 493, 897, 921
RsaNI	GTAC	5 cut(s)	242, 288, 492, 896, 920
RseI	CAYNNNNRTG	1 cut(s)	1581
SapI	GCTCTTC	1 cut(s)	1176
SaqAI	TTAA	6 cut(s)	891, 1022, 1068, 1152, 1649, 1749
SatI	GCNGC	7 cut(s)	9, 178, 198, 260, 263, 911, 1433
Sau3AI	GATC	5 cut(s)	478, 592, 1058, 1126, 1510
Sau96I	GGNCC	2 cut(s)	131, 1052
SchI	GAGTC	2 cut(s)	791, 871
ScrFI	CCNGG	4 cut(s)	384, 465, 1056, 1248
SfaNI	GCATC	5 cut(s)	747, 949, 985, 1076, 1450
SfcI	CTRYAG	1 cut(s)	329
SfuI	TTCGAA	1 cut(s)	1143
SinI	GGWCC	2 cut(s)	131, 1052
SmiMI	CAYNNNNRTG	1 cut(s)	1581
SmlI	CTYRAG	2 cut(s)	70, 1067
SmoI	CTYRAG	2 cut(s)	70, 1067
SsiI	CCGC	7 cut(s)	9, 178, 197, 520, 760, 815, 910
SspI	AATATT	2 cut(s)	821, 1317
SspMI	CTAG	4 cut(s)	140, 426, 590, 1379
StyD4I	CCNGG	4 cut(s)	382, 463, 1054, 1246
StyI	CCWWGG	3 cut(s)	618, 1469, 1665
TaaI	ACNGT	5 cut(s)	44, 220, 365, 1236, 1580
TaiI	ACGT	1 cut(s)	186
TaqI	TCGA	6 cut(s)	534, 605, 764, 1129, 1143, 1341
TatI	WGTACW	2 cut(s)	241, 919
TauI	GCSGC	4 cut(s)	11, 180, 200, 913
TfiI	GAWTC	4 cut(s)	157, 271, 1241, 1310
Tru1I	TTAA	6 cut(s)	891, 1022, 1068, 1152, 1649, 1749
Tru9I	TTAA	6 cut(s)	891, 1022, 1068, 1152, 1649, 1749
TscAI	CASTG	3 cut(s)	377, 1005, 1576
TseI	GCWGC	3 cut(s)	259, 262, 1432
TspDTI	ATGAA	5 cut(s)	326, 552, 702, 864, 1748
TspRI	CASTG	3 cut(s)	377, 1005, 1576
Van91I	CCANNNNNTGG	2 cut(s)	187, 1424
Vha464I	CTTAAG	1 cut(s)	1067
VpaK11BI	GGWCC	2 cut(s)	131, 1052
XagI	CCTNNNNNAGG	1 cut(s)	1047
XapI	RAATTY	7 cut(s)	514, 641, 647, 661, 773, 1076, 1596
XceI	RCATGY	4 cut(s)	617, 993, 1439, 1631
XspI	CTAG	4 cut(s)	140, 426, 590, 1379
Zsp2I	ATGCAT	1 cut(s)	1546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.