Rmu_co8333539.1_g000001

RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8333539.1
OG0012171
Physical Location & Seq
Forward (+)
1 .. 938
938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8333539.1_g000001.1.cds

Sequence Viewer

Length: 682 bp
cgaaacagagaggttgggcttcaagggtccgacgctagtccaagcccaagcgatcccggttattctctccggccgccacgtgctggtgaatgcggcgacggggacgggcaaaacggtggcgtatttggctccggttgtgcaccacctgagcttgcagcagcctcggattcagcgctccgatggtacttttgctttggttctcgtgccgacgagagagctgtgtctgcaggttcacgagattttgcacaagttactgcaccgttttcactggattgtgcctggttatgtgatgggaggtgaaaagagggaaaaggagaaggctagattgcgcaaaggcatatctattctcgttgcaactcctggaaggctgttggatcatttgaagcatacatcatcattttcgcacaccaatttgcggtggataattttcgatgaagcagataggattttggaacttggatttggaaaggagatagaggaaatactggatcttttgggttcgaggaaacatgccaaggatagtggtgcgtcaagtaaatttgaatttcagagacagaatttgctgttgtctgcgaccttgaatgaaaaagtcaatcatcttgcaaagatgagcttagagaaaccggtcttgattgggcaacaggctctacaaatgaagattataaacttccagcccaattga

Protein Analysis

226

Amino Acids

25.4

Weight (kDa)

9.81

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40700
fragaria_vesca FvH4_1g09140 FvH4_1g09140 FvH4_1g09140
malus_domestica MD02G1096800.v1.1
prunus_persica Prupe.7G194100_v2.0.a1
pyrus_communis pycom02g07680 pycom15g19610
rosa_chinensis RchiOBHm_Chr2g0095531
rosa_laevigata RLG00000016576
rosa_multiflora Rmu_co8308315.1_g000001 Rmu_co8333539.1_g000001
rosa_roxburghii Rroxscaffold_2G00146220
rosa_rugosa Rorug02G0052200
rosa_samantha Rh2AG100000 Rh2BG101400 Rh2CG103600 Rh2DG100900
rosa_wichuraiana Rw2G007590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 663
Acc16I TGCGCA 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 83
AciI CCGC 3 cut(s) 74, 93, 416
AclWI GGATC 3 cut(s) 47, 382, 496
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 3 cut(s) 537, 543, 557
AcvI CACGTG 1 cut(s) 80
AfaI GTAC 1 cut(s) 185
AfeI AGCGCT 1 cut(s) 174
AfiI CCNNNNNNNGG 2 cut(s) 83, 415
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 4 cut(s) 23, 383, 543, 581
AjnI CCWGG 2 cut(s) 278, 359
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 151, 218, 613
AluI AGCT 3 cut(s) 151, 218, 613
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 545
Alw44I GTGCAC 1 cut(s) 138
AlwI GGATC 3 cut(s) 47, 382, 496
Aor51HI AGCGCT 1 cut(s) 174
AoxI GGCC 1 cut(s) 71
ApaLI GTGCAC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 155, 158
ApoI RAATTY 3 cut(s) 537, 543, 557
AsiGI ACCGGT 1 cut(s) 623
AspLEI GCGC 2 cut(s) 175, 331
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 98, 309
AvaII GGWCC 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 142
BauI CACGAG 2 cut(s) 201, 234
BbrPI CACGTG 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 167, 170
BccI CCATC 2 cut(s) 174, 284
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BciT130I CCWGG 2 cut(s) 280, 361
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 545
BfaI CTAG 2 cut(s) 36, 322
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 218
BisI GCNGC 4 cut(s) 74, 94, 156, 159
BlsI GCNGC 4 cut(s) 75, 95, 157, 160
Bme1390I CCNGG 3 cut(s) 57, 280, 361
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 28, 130
BmrFI CCNGG 3 cut(s) 57, 280, 361
BoxI GACNNNNGTC 1 cut(s) 36
Bpu10I CCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 162, 514
BsaWI WCCGGW 2 cut(s) 131, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 415
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 2 cut(s) 273, 490
BseBI CCWGG 2 cut(s) 280, 361
BseDI CCNNGG 2 cut(s) 162, 514
BseLI CCNNNNNNNGG 2 cut(s) 83, 415
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 2 cut(s) 273, 490
BseSI GKGCMC 1 cut(s) 142
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 167, 170
BsgI GTGCAG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 73
BshTI ACCGGT 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 4 cut(s) 57, 70, 132, 624
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 83, 415
BsmAI GTCTC 1 cut(s) 545
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 3 cut(s) 52, 374, 488
BspACI CCGC 3 cut(s) 74, 93, 416
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 28, 130
BspMAI CTGCAG 1 cut(s) 229
BspMI ACCTGC 1 cut(s) 218
BspPI GGATC 3 cut(s) 47, 382, 496
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 2 cut(s) 273, 490
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 162, 514
BssMI GATC 3 cut(s) 52, 374, 488
BssSI CACGAG 2 cut(s) 201, 234
BssT1I CCWWGG 1 cut(s) 514
Bst2BI CACGAG 2 cut(s) 201, 234
Bst2UI CCWGG 2 cut(s) 280, 361
Bst4CI ACNGT 2 cut(s) 116, 261
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 2 cut(s) 147, 614
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 331
BstKTI GATC 3 cut(s) 55, 377, 491
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 3 cut(s) 52, 374, 488
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 2 cut(s) 126, 224
BstNI CCWGG 2 cut(s) 280, 361
BstNSI RCATGY 1 cut(s) 513
BstPAI GACNNNNGTC 1 cut(s) 36
BstSCI CCNGG 3 cut(s) 55, 278, 359
BstSFI CTRYAG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 2 cut(s) 167, 170
BstX2I RGATCY 1 cut(s) 488
BstYI RGATCY 1 cut(s) 488
BstZI CGGCCG 1 cut(s) 71
BsuRI GGCC 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 266
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 2 cut(s) 175, 331
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 2 cut(s) 41, 517
Csp6I GTAC 1 cut(s) 184
CspAI ACCGGT 1 cut(s) 623
CspCI CAANNNNNGTGG 2 cut(s) 503, 538
CviAII CATG 1 cut(s) 510
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 2 cut(s) 147, 614
DpnI GATC 3 cut(s) 54, 376, 490
DpnII GATC 3 cut(s) 52, 374, 488
EaeI YGGCCR 1 cut(s) 71
EagI CGGCCG 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 174
Eco52I CGGCCG 1 cut(s) 71
Eco72I CACGTG 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 278, 359
EcoT14I CCWWGG 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 1 cut(s) 513
FaiI YATR 5 cut(s) 286, 339, 388, 511, 663
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 116
FatI CATG 1 cut(s) 509
Fnu4HI GCNGC 4 cut(s) 74, 94, 156, 159
Fsp4HI GCNGC 4 cut(s) 74, 94, 156, 159
FspBI CTAG 2 cut(s) 36, 322
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 2 cut(s) 174, 330
GluI GCNGC 4 cut(s) 74, 94, 156, 159
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 73
HapII CCGG 4 cut(s) 57, 70, 132, 624
HgaI GACGC 2 cut(s) 41, 517
HhaI GCGC 2 cut(s) 175, 331
Hin1II CATG 1 cut(s) 513
Hin6I GCGC 2 cut(s) 173, 329
HinP1I GCGC 2 cut(s) 173, 329
HinfI GANTC 1 cut(s) 167
HpaII CCGG 4 cut(s) 57, 70, 132, 624
HphI GGTGA 2 cut(s) 98, 309
Hpy166II GTNNAC 2 cut(s) 140, 233
Hpy188I TCNGA 4 cut(s) 31, 166, 179, 550
Hpy188III TCNNGA 2 cut(s) 234, 629
Hpy8I GTNNAC 2 cut(s) 140, 233
Hpy99I CGWCG 3 cut(s) 35, 101, 212
HpyAV CCTTC 2 cut(s) 311, 358
HpyCH4III ACNGT 2 cut(s) 116, 261
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 7 cut(s) 140, 155, 227, 245, 257, 354, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 224
HpyF3I CTNAG 2 cut(s) 147, 614
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 1 cut(s) 513
HspAI GCGC 2 cut(s) 173, 329
Kzo9I GATC 3 cut(s) 52, 374, 488
LmnI GCTCC 2 cut(s) 134, 180
Lsp1109I GCAGC 2 cut(s) 167, 170
MaeI CTAG 2 cut(s) 36, 322
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 250
MalI GATC 3 cut(s) 54, 376, 490
MboI GATC 3 cut(s) 52, 374, 488
MboII GAAGA 1 cut(s) 668
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 1 cut(s) 488
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 6 cut(s) 410, 424, 537, 543, 557, 677
MmeI TCCRAC 2 cut(s) 54, 352
MnlI CCTC 6 cut(s) 4, 172, 288, 298, 470, 496
MspI CCGG 4 cut(s) 57, 70, 132, 624
MspR9I CCNGG 3 cut(s) 57, 280, 361
MunI CAATTG 1 cut(s) 677
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 2 cut(s) 280, 361
MwoI GCNNNNNNNGC 2 cut(s) 126, 224
NciI CCSGG 1 cut(s) 57
NdeII GATC 3 cut(s) 52, 374, 488
NlaIII CATG 1 cut(s) 513
NlaIV GGNNCC 2 cut(s) 28, 130
NsbI TGCGCA 1 cut(s) 330
NspI RCATGY 1 cut(s) 513
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 4 cut(s) 75, 95, 157, 160
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 80
PshAI GACNNNNGTC 1 cut(s) 36
PsiI TTATAA 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 278, 359
PspCI CACGTG 1 cut(s) 80
PspGI CCWGG 2 cut(s) 278, 359
PspN4I GGNNCC 2 cut(s) 28, 130
PspPI GGNCC 1 cut(s) 27
PstI CTGCAG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 488
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SatI GCNGC 4 cut(s) 74, 94, 156, 159
Sau3AI GATC 3 cut(s) 52, 374, 488
Sau96I GGNCC 1 cut(s) 27
ScrFI CCNGG 3 cut(s) 57, 280, 361
SduI GDGCHC 1 cut(s) 142
SetI ASST 9 cut(s) 15, 82, 148, 153, 220, 232, 299, 579, 615
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 27
Sse9I AATT 6 cut(s) 410, 424, 537, 543, 557, 677
SsiI CCGC 3 cut(s) 74, 93, 416
SspMI CTAG 2 cut(s) 36, 322
StyD4I CCNGG 3 cut(s) 55, 278, 359
StyI CCWWGG 1 cut(s) 514
TaaI ACNGT 2 cut(s) 116, 261
TaiI ACGT 1 cut(s) 82
TaqI TCGA 2 cut(s) 430, 501
TasI AATT 6 cut(s) 410, 424, 537, 543, 557, 677
TauI GCSGC 2 cut(s) 76, 96
TfiI GAWTC 1 cut(s) 167
TscAI CASTG 1 cut(s) 273
TseI GCWGC 2 cut(s) 155, 158
TspDTI ATGAA 3 cut(s) 448, 598, 669
TspRI CASTG 1 cut(s) 273
Van91I CCANNNNNTGG 1 cut(s) 83
VneI GTGCAC 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 3 cut(s) 537, 543, 557
XceI RCATGY 1 cut(s) 513
XspI CTAG 2 cut(s) 36, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.