FvH4_1g09320

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017963
Physical Location & Seq
Reverse (-)
4999415 .. 5000736
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09320.t1

Sequence Viewer

Length: 345 bp
ATGATTAAAAGGAATGAAGAATTAGTACCAGGTGAGAAGAAGAGGGTTTTAAAGTCTCCAATGAGAAGGAGGAGGACTCCGATGTTGCCGGGTGACCGGAATTTGGTTCAAGAAGGTAAATTTTGGGCTGACGTTGTTGTTGCTAGTGATGGACGTGGAGATTGTGAGACTGTTGGTGAAGCATTGAAGAAGGCTAATAATAGCGAGTACAGATTTGTGATTAGAATCAAGGCCGGGGTTTACAATGAGACTGTGGAAGTGTCAAGAGAAAAGGTTAATATAATGTTGGTTGGAGAAGGGATGAACAAGACCTTAAGCACAGGGTCCCAAGAGTTTTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

115

Amino Acids

12.93

Weight (kDa)

9.59

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 44 - 112 3.5e-20 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 100, 119
AfaI GTAC 2 cut(s) 27, 209
AfiI CCNNNNNNNGG 1 cut(s) 103
AflII CTTAAG 1 cut(s) 313
AgsI TTSAA 2 cut(s) 110, 187
AjiI CACGTC 1 cut(s) 155
AjnI CCWGG 1 cut(s) 28
Alw26I GTCTC 3 cut(s) 60, 161, 242
AoxI GGCC 1 cut(s) 231
ApoI RAATTY 2 cut(s) 100, 119
AspS9I GGNCC 1 cut(s) 324
AsuC2I CCSGG 2 cut(s) 90, 235
AsuHPI GGTGA 3 cut(s) 44, 104, 188
AvaII GGWCC 1 cut(s) 324
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BccI CCATC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 30
BcnI CCSGG 2 cut(s) 90, 235
BcoDI GTCTC 3 cut(s) 60, 161, 242
BfaI CTAG 1 cut(s) 144
BfrI CTTAAG 1 cut(s) 313
Bme1390I CCNGG 3 cut(s) 30, 90, 235
Bme18I GGWCC 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 324
BmiI GGNNCC 2 cut(s) 325, 326
BmrFI CCNGG 3 cut(s) 30, 90, 235
BplI GAGNNNNNCTC 2 cut(s) 61, 93
BpuMI CCSGG 2 cut(s) 90, 235
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 85
BshFI GGCC 1 cut(s) 233
BsiSI CCGG 3 cut(s) 89, 97, 234
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 3 cut(s) 60, 161, 242
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 1 cut(s) 233
BspANI GGCC 1 cut(s) 233
BspLI GGNNCC 2 cut(s) 325, 326
BspTI CTTAAG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 172, 253
Bst6I CTCTTC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 313
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 1 cut(s) 306
BstMAI GTCTC 3 cut(s) 60, 161, 242
BstNI CCWGG 1 cut(s) 30
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 3 cut(s) 28, 88, 233
BsuRI GGCC 1 cut(s) 233
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 324
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 2 cut(s) 26, 208
CviJI RGCY 3 cut(s) 128, 194, 233
CviKI_1 RGCY 3 cut(s) 128, 194, 233
CviQI GTAC 2 cut(s) 26, 208
DraI TTTAAA 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 324
EcoO65I GGTNACC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 28
FaiI YATR 1 cut(s) 281
FaqI GGGAC 1 cut(s) 310
FokI GGATG 1 cut(s) 313
FspBI CTAG 1 cut(s) 144
HaeIII GGCC 1 cut(s) 233
HapII CCGG 3 cut(s) 89, 97, 234
HinfI GANTC 2 cut(s) 76, 225
HpaII CCGG 3 cut(s) 89, 97, 234
HphI GGTGA 3 cut(s) 44, 104, 188
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 81
Hpy188III TCNNGA 2 cut(s) 110, 264
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 4 cut(s) 60, 107, 184, 290
HpyCH4III ACNGT 2 cut(s) 172, 253
HpyCH4IV ACGT 2 cut(s) 132, 154
HpyCH4V TGCA 1 cut(s) 339
HpySE526I ACGT 2 cut(s) 132, 154
KflI GGGWCCC 1 cut(s) 324
LpnPI CCDG 6 cut(s) 15, 42, 102, 110, 247, 306
MabI ACCWGGT 1 cut(s) 28
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 132, 154
MaeIII GTNAC 1 cut(s) 92
MboII GAAGA 4 cut(s) 29, 49, 52, 199
MluCI AATT 3 cut(s) 20, 100, 119
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 271
MnlI CCTC 3 cut(s) 36, 63, 66
MseI TTAA 4 cut(s) 6, 50, 276, 314
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 3 cut(s) 89, 97, 234
MspR9I CCNGG 3 cut(s) 30, 90, 235
MvaI CCWGG 1 cut(s) 30
NciI CCSGG 2 cut(s) 90, 235
NlaIV GGNNCC 2 cut(s) 325, 326
NmuCI GTSAC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 225
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 28
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 325, 326
PspPI GGNCC 1 cut(s) 324
PspPPI RGGWCCY 1 cut(s) 324
RsaI GTAC 2 cut(s) 27, 209
RsaNI GTAC 2 cut(s) 26, 208
SaqAI TTAA 4 cut(s) 6, 50, 276, 314
Sau96I GGNCC 1 cut(s) 324
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 3 cut(s) 30, 90, 235
SetI ASST 6 cut(s) 34, 118, 135, 157, 276, 314
SexAI ACCWGGT 1 cut(s) 28
SinI GGWCC 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 3 cut(s) 20, 100, 119
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 3 cut(s) 28, 88, 233
TaaI ACNGT 2 cut(s) 172, 253
TaiI ACGT 2 cut(s) 135, 157
TasI AATT 3 cut(s) 20, 100, 119
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 4 cut(s) 6, 50, 276, 314
Tru9I TTAA 4 cut(s) 6, 50, 276, 314
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 30, 317
Vha464I CTTAAG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 2 cut(s) 100, 119
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.