MD02G1100400.v1.1

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017963
Physical Location & Seq
Reverse (-)
7997673 .. 7999718
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1100400.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGAACAAGCTCATCATCTTCTTTCCCTCCTTAACCTCTCTGCTTCTAATTTCTTCCTTTTCCGCCATTCTACATCCATCTCTCTGTTCCTCCGCCCACGCCTCCGCCCCATTCCACAACCACTTCACCGCATTCCACCACTTCCTCGAGACCAACATCAACCGAACCGCAGCGTCAATTGGAACAACCCAGCAGAGCATCAGAGCCCTTTTGAGGCTCCAAGAATTGTATGTCCAAGAAAGGAATGCGCTGAGAGATTGCTTGGAGATGTTAGACCAAACCCTTTACGAACTAGGCCAGGCAATCGACAACTTGCACGGTTTGCGTGTGTCTTACGGCAACCTCAAGACCCTTCTGAGCGCTGCAATGACGAATCAGAACACTTGCATTGATGGGTTCTTTGAGTTGGAGGAGTTGGACTCCCAAAACCAAAAGGGTCTCAAAGGTCATTTGCAAGGGCTGCTGAGTCCGATTTCCGAGTCGATGAGTAATTGCTTGGCAAGGATCAGAAACCAAGAACAGATTCTGAATTCGCAGAAGAGGGTTAGTAGCAGTCCTACCAAGAAATCATCGAAGAAGAGGTTTCCGAAATGGATGCTGCCGGGTGATCGAAATCTGATGCAGAGATTAGTGCCAAGAATAAGGGCTGACATTGTTGTTGCTGCCGATGGGAGTGGAGATTATGAAACCATTGGTGAAGCTCTGAAGATGGCTCCTAATTTTAGCATTGACAGATTTGTAATTAGAATAAAATCCGGGGTTTACAATGAGACTGTAGAGATCGCCAGAGAAAAGTCTAATATCATGCTTGTTGGAGAGGGGATGAAATCAACCATAATCACAGGGTCTAAGAGCTTTGCAGATGGATTTTCCACCTTTGCTACAGCCACTTTAACTGTAATCGGGGACAAGTTCCTAGCTAGGGACCTGACGATAACCAACACAGCAGGCTCAGAAAAGTTCCAGGCAGTTGCTGCAAGAGTGACCTCAAACTCTGCGTTCTACCGCTGCAACTTCAGTTCATACCAAGACACCCTCTACGTCCACTCACTCCGCCAATTCTACCGCAACTGCATCATCGAAGGCACGATTGATTTCATTTTCGGTAATGCAGCTGCAGTCTTCCAACGCTGCAGGGTGATTGTCCGAAAACCAATCCCCGGACAGACCGTTATGATCACTGCACAAGGCAGAACAGACCCGAATCAGAACACCGGCATTTCACTGCAGAAATGCACCATTGTGGCTGCGCCGGAATTCAACAAGACCGAGAGGCAAACTTTCAGAGCATTTTTGGGAAGGCCATGGAGGAACTACTCAAGGACTGTTGTGATGAGGAGCTACTTGGGGGACTTCATACACCCACAGGGGTGGTCAAAGTGGGATGAATTTACCACAGTGGACACGGTGGAGTACATTGAGTACTTCAACTTTGGACCTGGATCGGACACCAAGCAGAGGGTAAATTGGGGAGGGTATAAAAGGAATTGCAGTGAGGAGATTGTGAGGCAGTTTACGGTTGGCTTGTTTTTGCATGGTGAAAGTGATTGGATTCAGACCACTGGGATTCCACTCTCCTATGGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

530

Amino Acids

59.48

Weight (kDa)

9.15

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 23 - 167 3.4e-20 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 217 - 513 1.8e-127 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 63, 93, 105, 129, 168, 1006, 1054, 1066
AclWI GGATC 2 cut(s) 512, 1450
AcsI RAATTY 3 cut(s) 529, 1256, 1388
AcuI CTGAAG 2 cut(s) 725, 1000
AfaI GTAC 2 cut(s) 1415, 1424
AfeI AGCGCT 1 cut(s) 361
AfiI CCNNNNNNNGG 4 cut(s) 213, 922, 1160, 1458
AgsI TTSAA 2 cut(s) 1261, 1429
AjnI CCWGG 3 cut(s) 297, 963, 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 507, 539
AleI CACNNNNGTG 2 cut(s) 1241, 1369
AluBI AGCT 6 cut(s) 10, 701, 855, 920, 1115, 1341
AluI AGCT 6 cut(s) 10, 701, 855, 920, 1115, 1341
Alw26I GTCTC 3 cut(s) 143, 443, 764
AlwI GGATC 2 cut(s) 512, 1450
AlwNI CAGNNNCTG 2 cut(s) 526, 974
Ama87I CYCGRG 1 cut(s) 146
Aor51HI AGCGCT 1 cut(s) 361
AoxI GGCC 2 cut(s) 295, 1301
ApoI RAATTY 3 cut(s) 529, 1256, 1388
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 3 cut(s) 250, 362, 1252
AspS9I GGNCC 2 cut(s) 925, 1436
AsuC2I CCSGG 3 cut(s) 603, 757, 1161
AsuHPI GGTGA 5 cut(s) 118, 617, 707, 1150, 1550
AvaI CYCGRG 1 cut(s) 146
AvaII GGWCC 2 cut(s) 925, 1436
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 1114
BccI CCATC 5 cut(s) 85, 386, 662, 703, 857
BceAI ACGGC 1 cut(s) 352
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 3 cut(s) 299, 965, 1440
BclI TGATCA 1 cut(s) 1176
BcnI CCSGG 3 cut(s) 603, 757, 1161
BcoDI GTCTC 3 cut(s) 143, 443, 764
BfaI CTAG 3 cut(s) 293, 917, 921
BfmI CTRYAG 5 cut(s) 774, 882, 1116, 1132, 1226
BfoI RGCGCY 1 cut(s) 363
BmcAI AGTACT 1 cut(s) 1424
Bme1390I CCNGG 6 cut(s) 299, 603, 757, 965, 1161, 1440
Bme18I GGWCC 2 cut(s) 925, 1436
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 925, 1436
BmiI GGNNCC 3 cut(s) 218, 714, 926
BmrFI CCNGG 6 cut(s) 299, 603, 757, 965, 1161, 1440
BmrI ACTGGG 1 cut(s) 1572
BmsI GCATC 4 cut(s) 207, 585, 609, 1083
BmuI ACTGGG 1 cut(s) 1572
BpiI GAAGAC 1 cut(s) 1114
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpuEI CTTGAG 2 cut(s) 329, 1303
BpuMI CCSGG 3 cut(s) 603, 757, 1161
BsaBI GATNNNNATC 1 cut(s) 612
BsaI GGTCTC 2 cut(s) 143, 443
BsaJI CCNNGG 3 cut(s) 756, 1159, 1304
Bsc4I CCNNNNNNNGG 4 cut(s) 213, 922, 1160, 1458
Bse118I RCCGGY 1 cut(s) 1214
Bse1I ACTGG 1 cut(s) 1567
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 3 cut(s) 299, 965, 1440
BseDI CCNNGG 3 cut(s) 756, 1159, 1304
BseGI GGATG 4 cut(s) 73, 600, 828, 1390
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 4 cut(s) 213, 922, 1160, 1458
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 4 cut(s) 242, 347, 455, 966
BseNI ACTGG 1 cut(s) 1567
BseRI GAGGAG 3 cut(s) 425, 1351, 1511
BseYI CCCAGC 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 1167
BshFI GGCC 2 cut(s) 297, 1303
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 5 cut(s) 602, 756, 1161, 1215, 1253
BslFI GGGAC 3 cut(s) 920, 938, 1364
BslI CCNNNNNNNGG 4 cut(s) 213, 922, 1160, 1458
BsmAI GTCTC 3 cut(s) 143, 443, 764
BsmFI GGGAC 3 cut(s) 920, 938, 1364
BsmI GAATGC 2 cut(s) 131, 250
BsnI GGCC 2 cut(s) 297, 1303
Bso31I GGTCTC 2 cut(s) 143, 443
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 5 cut(s) 504, 607, 780, 1176, 1442
Bsp19I CCATGG 1 cut(s) 1304
BspACI CCGC 8 cut(s) 63, 93, 105, 129, 168, 1006, 1054, 1066
BspANI GGCC 2 cut(s) 297, 1303
BspCNI CTCAG 4 cut(s) 243, 348, 456, 965
BspLI GGNNCC 3 cut(s) 218, 714, 926
BspMAI CTGCAG 3 cut(s) 1120, 1136, 1230
BspPI GGATC 2 cut(s) 512, 1450
BspTNI GGTCTC 2 cut(s) 143, 443
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 1214
BsrI ACTGG 1 cut(s) 1567
BssAI RCCGGY 1 cut(s) 1214
BssECI CCNNGG 3 cut(s) 756, 1159, 1304
BssMI GATC 5 cut(s) 504, 607, 780, 1176, 1442
BssT1I CCWWGG 1 cut(s) 1304
Bst2UI CCWGG 3 cut(s) 299, 965, 1440
Bst4CI ACNGT 8 cut(s) 320, 775, 898, 1171, 1327, 1399, 1408, 1519
Bst6I CTCTTC 2 cut(s) 533, 572
BstAPI GCANNNNNTGC 3 cut(s) 322, 460, 974
BstC8I GCNNGC 1 cut(s) 949
BstDEI CTNAG 5 cut(s) 251, 356, 464, 849, 952
BstDSI CCRYGG 1 cut(s) 1304
BstF5I GGATG 4 cut(s) 73, 600, 828, 1390
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 3 cut(s) 250, 362, 1252
BstKTI GATC 5 cut(s) 507, 610, 783, 1179, 1445
BstMAI GTCTC 3 cut(s) 143, 443, 764
BstMBI GATC 5 cut(s) 504, 607, 780, 1176, 1442
BstMWI GCNNNNNNNGC 3 cut(s) 322, 460, 974
BstNI CCWGG 3 cut(s) 299, 965, 1440
BstSCI CCNGG 6 cut(s) 297, 601, 755, 963, 1159, 1438
BstSFI CTRYAG 5 cut(s) 774, 882, 1116, 1132, 1226
BstV2I GAAGAC 1 cut(s) 1114
BstXI CCANNNNNNTGG 1 cut(s) 1371
BsuRI GGCC 2 cut(s) 297, 1303
BtgI CCRYGG 1 cut(s) 1304
BtsCI GGATG 4 cut(s) 73, 600, 828, 1390
BtsI GCAGTG 3 cut(s) 1179, 1223, 1498
BtsIMutI CAGTG 5 cut(s) 1179, 1223, 1404, 1498, 1560
Cac8I GCNNGC 1 cut(s) 949
CaiI CAGNNNCTG 2 cut(s) 526, 974
CfoI GCGC 3 cut(s) 250, 362, 1252
Cfr10I RCCGGY 1 cut(s) 1214
Cfr13I GGNCC 2 cut(s) 925, 1436
CseI GACGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 1414, 1423
CviAII CATG 3 cut(s) 805, 1305, 1535
CviQI GTAC 2 cut(s) 1414, 1423
DdeI CTNAG 5 cut(s) 251, 356, 464, 849, 952
DpnI GATC 5 cut(s) 506, 609, 782, 1178, 1444
DpnII GATC 5 cut(s) 504, 607, 780, 1176, 1442
Eam1104I CTCTTC 2 cut(s) 533, 572
EarI CTCTTC 2 cut(s) 533, 572
EciI GGCGGA 4 cut(s) 52, 82, 94, 1043
Eco130I CCWWGG 1 cut(s) 1304
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 2 cut(s) 143, 443
Eco47I GGWCC 2 cut(s) 925, 1436
Eco47III AGCGCT 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 725, 1000
Eco88I CYCGRG 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 925
EcoRI GAATTC 2 cut(s) 529, 1256
EcoRII CCWGG 3 cut(s) 297, 963, 1438
EcoT14I CCWWGG 1 cut(s) 1304
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 1304
FaeI CATG 3 cut(s) 808, 1308, 1538
FaqI GGGAC 3 cut(s) 920, 938, 1364
FatI CATG 3 cut(s) 804, 1304, 1534
FbaI TGATCA 1 cut(s) 1176
FokI GGATG 4 cut(s) 60, 607, 835, 1397
FriOI GRGCYC 1 cut(s) 208
FspBI CTAG 3 cut(s) 293, 917, 921
GlaI GCGC 3 cut(s) 249, 361, 1251
GsaI CCCAGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 363
HaeIII GGCC 2 cut(s) 297, 1303
HapII CCGG 5 cut(s) 602, 756, 1161, 1215, 1253
HgaI GACGC 1 cut(s) 162
HhaI GCGC 3 cut(s) 250, 362, 1252
Hin1II CATG 3 cut(s) 808, 1308, 1538
Hin6I GCGC 3 cut(s) 248, 360, 1250
HinP1I GCGC 3 cut(s) 248, 360, 1250
HinfI GANTC 8 cut(s) 373, 419, 466, 479, 523, 1204, 1552, 1567
HpaII CCGG 5 cut(s) 602, 756, 1161, 1215, 1253
HphI GGTGA 5 cut(s) 118, 617, 707, 1150, 1550
Hpy166II GTNNAC 4 cut(s) 763, 1045, 1402, 1515
Hpy188III TCNNGA 2 cut(s) 148, 346
Hpy8I GTNNAC 4 cut(s) 763, 1045, 1402, 1515
HpyAV CCTTC 3 cut(s) 362, 1076, 1293
HpyCH4III ACNGT 8 cut(s) 320, 775, 898, 1171, 1327, 1399, 1408, 1519
HpyCH4IV ACGT 1 cut(s) 1041
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 460, 974
HpyF3I CTNAG 5 cut(s) 251, 356, 464, 849, 952
HpySE526I ACGT 1 cut(s) 1041
Hsp92II CATG 3 cut(s) 808, 1308, 1538
HspAI GCGC 3 cut(s) 248, 360, 1250
Ksp22I TGATCA 1 cut(s) 1176
Kzo9I GATC 5 cut(s) 504, 607, 780, 1176, 1442
LmnI GCTCC 3 cut(s) 222, 718, 1338
LweI GCATC 4 cut(s) 207, 585, 609, 1083
MaeI CTAG 3 cut(s) 293, 917, 921
MaeII ACGT 1 cut(s) 1041
MaeIII GTNAC 1 cut(s) 982
MalI GATC 5 cut(s) 506, 609, 782, 1178, 1444
MboI GATC 5 cut(s) 504, 607, 780, 1176, 1442
MboII GAAGA 7 cut(s) 10, 45, 550, 586, 589, 718, 1114
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 208
MlyI GAGTC 3 cut(s) 413, 475, 488
MmeI TCCRAC 4 cut(s) 387, 396, 793, 1150
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 2 cut(s) 32, 893
MslI CAYNNNNRTG 2 cut(s) 1241, 1369
MspA1I CMGCKG 2 cut(s) 1008, 1115
MspI CCGG 5 cut(s) 602, 756, 1161, 1215, 1253
MspR9I CCNGG 6 cut(s) 299, 603, 757, 965, 1161, 1440
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 2 cut(s) 131, 250
MvaI CCWGG 3 cut(s) 299, 965, 1440
MwoI GCNNNNNNNGC 3 cut(s) 322, 460, 974
NciI CCSGG 3 cut(s) 603, 757, 1161
NcoI CCATGG 1 cut(s) 1304
NdeII GATC 5 cut(s) 504, 607, 780, 1176, 1442
NlaIII CATG 3 cut(s) 808, 1308, 1538
NlaIV GGNNCC 3 cut(s) 218, 714, 926
NmuCI GTSAC 1 cut(s) 982
OliI CACNNNNGTG 2 cut(s) 1241, 1369
PaeR7I CTCGAG 1 cut(s) 146
PctI GAATGC 2 cut(s) 131, 250
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 5 cut(s) 373, 523, 1204, 1552, 1567
PleI GAGTC 3 cut(s) 413, 474, 487
PpsI GAGTC 3 cut(s) 413, 474, 487
PpuMI RGGWCCY 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 925
Psp6I CCWGG 3 cut(s) 297, 963, 1438
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 3 cut(s) 297, 963, 1438
PspN4I GGNNCC 3 cut(s) 218, 714, 926
PspPI GGNCC 2 cut(s) 925, 1436
PspPPI RGGWCCY 1 cut(s) 925
PstI CTGCAG 3 cut(s) 1120, 1136, 1230
PstNI CAGNNNCTG 2 cut(s) 526, 974
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 2 cut(s) 1415, 1424
RsaNI GTAC 2 cut(s) 1414, 1423
RseI CAYNNNNRTG 2 cut(s) 1241, 1369
SaqAI TTAA 2 cut(s) 32, 893
Sau3AI GATC 5 cut(s) 504, 607, 780, 1176, 1442
Sau96I GGNCC 2 cut(s) 925, 1436
ScaI AGTACT 1 cut(s) 1424
SchI GAGTC 3 cut(s) 413, 475, 488
ScrFI CCNGG 6 cut(s) 299, 603, 757, 965, 1161, 1440
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 4 cut(s) 207, 585, 609, 1083
SfcI CTRYAG 5 cut(s) 774, 882, 1116, 1132, 1226
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 2 cut(s) 925, 1436
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 2 cut(s) 1241, 1369
SmlI CTYRAG 3 cut(s) 146, 344, 1318
SmoI CTYRAG 3 cut(s) 146, 344, 1318
SsiI CCGC 8 cut(s) 63, 93, 105, 129, 168, 1006, 1054, 1066
SspMI CTAG 3 cut(s) 293, 917, 921
StyD4I CCNGG 6 cut(s) 297, 601, 755, 963, 1159, 1438
StyI CCWWGG 1 cut(s) 1304
TaaI ACNGT 8 cut(s) 320, 775, 898, 1171, 1327, 1399, 1408, 1519
TaiI ACGT 1 cut(s) 1044
TaqI TCGA 6 cut(s) 147, 306, 482, 572, 610, 1080
TaqII GACCGA 1 cut(s) 1283
TatI WGTACW 2 cut(s) 1413, 1422
TfiI GAWTC 5 cut(s) 373, 523, 1204, 1552, 1567
Tru1I TTAA 2 cut(s) 32, 893
Tru9I TTAA 2 cut(s) 32, 893
TscAI CASTG 5 cut(s) 1186, 1230, 1404, 1498, 1567
TseFI GTSAC 1 cut(s) 982
Tsp45I GTSAC 1 cut(s) 982
TspDTI ATGAA 8 cut(s) 17, 699, 839, 1011, 1087, 1345, 1401, 1575
TspRI CASTG 5 cut(s) 1186, 1230, 1404, 1498, 1567
VpaK11BI GGWCC 2 cut(s) 925, 1436
XapI RAATTY 3 cut(s) 529, 1256, 1388
XhoI CTCGAG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 3 cut(s) 293, 917, 921
ZrmI AGTACT 1 cut(s) 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.