RLG00000016604

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017963
Physical Location & Seq
Reverse (-)
8161951 .. 8163175
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016604

Sequence Viewer

Length: 756 bp
ATGATTAAAAACAATGAAGAATCAGTACCAAGTGAGCAGAAGAGGGTTATAAGTACCAAGAAGTCTTCAAAGAAAAGGAGGAGAATGCCACATTGGATGTTGCCCGGTGACCGGAATTTGGTTCAGAAAGAAGGTACAATTTGGCCTGACATTGTTGTTGCTGGTGATGGAAGTGGAGAATGTGAGACTATTGGTGAAGCATTGGTGATGGCTCCTAATAATAGGTCCAAGAATTTTGCAGATGGGTTTTCAACTTTTGCCACAGCTACTTTAAAAGTTACATTCTTTGCAGCTGTAATTGGGGACAGGTTTTTGGCTAGGGACTTGACCATTACCAACACAGCAGGGGAGAACAGACCCGAACCAAAACACGGCATCTCATTGCAGAATTGTACCATAGTGGCTGCGCCTGATTTCAGCAAGGCTGAGAGGAAAAACTTCTCCACTTTTTTGGGTAGGCCTAGGAGGAGCTATTCAACAGTGATGAAGAGCTACACAGGGGACATGATACACCCACAAAGGCGGTCAAAGTGGGATGAGTATAGTAGAATGGACACTGTCGAGTACATTGAGTACTTGAATTTTGGACCTGGATCAGACGCAATGCATAGGGTCAAATGGAGACGGTGTAGAAAGAATTGTACTGAAGAGATTGCGAAACAGTTCTCCGCCAGGTTGTTCTTGCACGAAGCAGGTGAATGGCTAGAGACCACAGGCATTCCGTCTTTCATGGTTCATGAGGAAGACAGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

252

Amino Acids

28.61

Weight (kDa)

9.0

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 120 - 229 2.7e-24 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 50
AarI CACCTGC 1 cut(s) 683
Acc36I ACCTGC 1 cut(s) 683
AciI CCGC 2 cut(s) 523, 669
AclWI GGATC 1 cut(s) 601
AcsI RAATTY 3 cut(s) 115, 232, 580
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 7 cut(s) 27, 55, 136, 394, 566, 575, 643
AfiI CCNNNNNNNGG 3 cut(s) 111, 118, 371
AgsI TTSAA 4 cut(s) 69, 252, 477, 580
AjnI CCWGG 2 cut(s) 589, 671
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 4 cut(s) 266, 293, 471, 492
AluI AGCT 4 cut(s) 266, 293, 471, 492
Alw26I GTCTC 3 cut(s) 179, 616, 701
AlwI GGATC 1 cut(s) 601
AlwNI CAGNNNCTG 1 cut(s) 753
AoxI GGCC 2 cut(s) 143, 458
ApeKI GCWGC 2 cut(s) 290, 404
ApoI RAATTY 3 cut(s) 115, 232, 580
Asp700I GAANNNNTTC 2 cut(s) 437, 662
AspA2I CCTAGG 1 cut(s) 461
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 2 cut(s) 225, 587
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 5 cut(s) 119, 176, 206, 217, 707
AvaII GGWCC 2 cut(s) 225, 587
AvrII CCTAGG 1 cut(s) 461
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 2 cut(s) 57, 750
BbvI GCAGC 2 cut(s) 302, 391
BccI CCATC 3 cut(s) 161, 202, 236
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 591, 673
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 3 cut(s) 179, 616, 701
BfaI CTAG 3 cut(s) 318, 462, 704
BfuAI ACCTGC 1 cut(s) 683
BisI GCNGC 2 cut(s) 291, 405
BlnI CCTAGG 1 cut(s) 461
BlsI GCNGC 2 cut(s) 292, 406
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 105, 591, 673
Bme18I GGWCC 2 cut(s) 225, 587
BmgT120I GGNCC 2 cut(s) 225, 587
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 3 cut(s) 105, 591, 673
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 2 cut(s) 57, 750
BpuMI CCSGG 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 118, 371
Bse3DI GCAATG 2 cut(s) 380, 609
BseBI CCWGG 2 cut(s) 591, 673
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 2 cut(s) 102, 541
BseLI CCNNNNNNNGG 3 cut(s) 111, 118, 371
BseMI GCAATG 2 cut(s) 380, 609
BseMII CTCAG 1 cut(s) 417
BseRI GAGGAG 2 cut(s) 94, 481
BseXI GCAGC 2 cut(s) 302, 391
BshFI GGCC 2 cut(s) 145, 460
BsiSI CCGG 2 cut(s) 105, 112
BslFI GGGAC 3 cut(s) 317, 335, 515
BslI CCNNNNNNNGG 3 cut(s) 111, 118, 371
BsmAI GTCTC 3 cut(s) 179, 616, 701
BsmBI CGTCTC 1 cut(s) 616
BsmFI GGGAC 3 cut(s) 317, 335, 515
BsmI GAATGC 2 cut(s) 90, 717
BsnI GGCC 2 cut(s) 145, 460
Bso31I GGTCTC 1 cut(s) 701
Bsp143I GATC 1 cut(s) 593
BspACI CCGC 2 cut(s) 523, 669
BspANI GGCC 2 cut(s) 145, 460
BspCNI CTCAG 1 cut(s) 418
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 1 cut(s) 601
BspQI GCTCTTC 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 380, 609
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 1 cut(s) 593
BssT1I CCWWGG 1 cut(s) 461
Bst2UI CCWGG 2 cut(s) 591, 673
Bst4CI ACNGT 5 cut(s) 481, 559, 627, 663, 749
Bst6I CTCTTC 3 cut(s) 35, 482, 642
BstDEI CTNAG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 2 cut(s) 102, 541
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 1 cut(s) 596
BstMAI GTCTC 3 cut(s) 179, 616, 701
BstMBI GATC 1 cut(s) 593
BstNI CCWGG 2 cut(s) 591, 673
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 3 cut(s) 103, 589, 671
BstV1I GCAGC 2 cut(s) 302, 391
BstV2I GAAGAC 2 cut(s) 57, 750
BstXI CCANNNNNNTGG 1 cut(s) 451
BsuRI GGCC 2 cut(s) 145, 460
BtsCI GGATG 2 cut(s) 102, 541
BtsIMutI CAGTG 3 cut(s) 486, 555, 754
BveI ACCTGC 1 cut(s) 683
CaiI CAGNNNCTG 1 cut(s) 753
CciI TCATGA 1 cut(s) 736
CfoI GCGC 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 225, 587
CseI GACGC 1 cut(s) 608
Csp6I GTAC 7 cut(s) 26, 54, 135, 393, 565, 574, 642
CviAII CATG 3 cut(s) 505, 730, 737
CviQI GTAC 7 cut(s) 26, 54, 135, 393, 565, 574, 642
DdeI CTNAG 1 cut(s) 426
DpnI GATC 1 cut(s) 595
DpnII GATC 1 cut(s) 593
DraI TTTAAA 1 cut(s) 273
Eam1104I CTCTTC 3 cut(s) 35, 482, 642
EarI CTCTTC 3 cut(s) 35, 482, 642
EciI GGCGGA 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 461
Eco147I AGGCCT 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 225, 587
Eco57I CTGAAG 1 cut(s) 666
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
EcoRII CCWGG 2 cut(s) 589, 671
EcoT14I CCWWGG 1 cut(s) 461
EcoT22I ATGCAT 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 616
FaeI CATG 3 cut(s) 508, 733, 740
FaiI YATR 7 cut(s) 50, 398, 506, 543, 609, 731, 738
FaqI GGGAC 3 cut(s) 317, 335, 515
FatI CATG 3 cut(s) 504, 729, 736
Fnu4HI GCNGC 2 cut(s) 291, 405
FokI GGATG 2 cut(s) 109, 548
Fsp4HI GCNGC 2 cut(s) 291, 405
FspBI CTAG 3 cut(s) 318, 462, 704
GlaI GCGC 1 cut(s) 408
GluI GCNGC 2 cut(s) 291, 405
HaeIII GGCC 2 cut(s) 145, 460
HapII CCGG 2 cut(s) 105, 112
HgaI GACGC 1 cut(s) 608
HhaI GCGC 1 cut(s) 409
Hin1II CATG 3 cut(s) 508, 733, 740
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HinfI GANTC 1 cut(s) 20
HpaII CCGG 2 cut(s) 105, 112
HphI GGTGA 5 cut(s) 119, 176, 206, 217, 707
Hpy188I TCNGA 2 cut(s) 126, 598
Hpy188III TCNNGA 1 cut(s) 737
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 5 cut(s) 481, 559, 627, 663, 749
HpyCH4V TGCA 5 cut(s) 239, 290, 385, 607, 685
HpyF3I CTNAG 1 cut(s) 426
Hsp92II CATG 3 cut(s) 508, 733, 740
HspAI GCGC 1 cut(s) 407
Kzo9I GATC 1 cut(s) 593
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 2 cut(s) 217, 468
Lsp1109I GCAGC 2 cut(s) 302, 391
LweI GCATC 1 cut(s) 384
MaeI CTAG 3 cut(s) 318, 462, 704
MaeIII GTNAC 3 cut(s) 107, 277, 749
MalI GATC 1 cut(s) 595
MboI GATC 1 cut(s) 593
MboII GAAGA 6 cut(s) 29, 52, 57, 499, 659, 755
MluCI AATT 7 cut(s) 115, 138, 232, 297, 388, 580, 637
MnlI CCTC 5 cut(s) 36, 72, 423, 459, 733
Mph1103I ATGCAT 1 cut(s) 609
MroXI GAANNNNTTC 2 cut(s) 437, 662
MseI TTAA 2 cut(s) 6, 272
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 105, 112
MspR9I CCNGG 3 cut(s) 105, 591, 673
Mva1269I GAATGC 2 cut(s) 90, 717
MvaI CCWGG 2 cut(s) 591, 673
NciI CCSGG 1 cut(s) 105
NdeII GATC 1 cut(s) 593
NlaIII CATG 3 cut(s) 508, 733, 740
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 107, 749
NsiI ATGCAT 1 cut(s) 609
PagI TCATGA 1 cut(s) 736
PaqCI CACCTGC 1 cut(s) 683
PceI AGGCCT 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 482
PctI GAATGC 2 cut(s) 90, 717
PdmI GAANNNNTTC 2 cut(s) 437, 662
PfeI GAWTC 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 557
PkrI GCNGC 2 cut(s) 292, 406
PsiI TTATAA 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 589, 671
PspEI GGTNACC 1 cut(s) 107
PspGI CCWGG 2 cut(s) 589, 671
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 225, 587
PstNI CAGNNNCTG 1 cut(s) 753
PsyI GACNNNGTC 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 7 cut(s) 27, 55, 136, 394, 566, 575, 643
RsaNI GTAC 7 cut(s) 26, 54, 135, 393, 565, 574, 642
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 2 cut(s) 6, 272
SatI GCNGC 2 cut(s) 291, 405
Sau3AI GATC 1 cut(s) 593
Sau96I GGNCC 2 cut(s) 225, 587
ScaI AGTACT 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 105, 591, 673
SfaNI GCATC 1 cut(s) 384
SinI GGWCC 2 cut(s) 225, 587
Sse9I AATT 7 cut(s) 115, 138, 232, 297, 388, 580, 637
SseBI AGGCCT 1 cut(s) 460
SsiI CCGC 2 cut(s) 523, 669
SspMI CTAG 3 cut(s) 318, 462, 704
StuI AGGCCT 1 cut(s) 460
StyD4I CCNGG 3 cut(s) 103, 589, 671
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 5 cut(s) 481, 559, 627, 663, 749
TaqI TCGA 1 cut(s) 561
TasI AATT 7 cut(s) 115, 138, 232, 297, 388, 580, 637
TatI WGTACW 3 cut(s) 564, 573, 641
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 6, 272
Tru9I TTAA 2 cut(s) 6, 272
TscAI CASTG 3 cut(s) 486, 562, 754
TseFI GTSAC 2 cut(s) 107, 749
TseI GCWGC 2 cut(s) 290, 404
Tsp45I GTSAC 2 cut(s) 107, 749
TspDTI ATGAA 4 cut(s) 30, 500, 718, 725
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 3 cut(s) 486, 562, 754
Tth111I GACNNNGTC 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 225, 587
XapI RAATTY 3 cut(s) 115, 232, 580
XmaJI CCTAGG 1 cut(s) 461
XmnI GAANNNNTTC 2 cut(s) 437, 662
XspI CTAG 3 cut(s) 318, 462, 704
ZrmI AGTACT 1 cut(s) 575
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.