Rroxscaffold_2G00145990

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017963
Physical Location & Seq
Forward (+)
83823640 .. 83825000
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145990.1

Sequence Viewer

Length: 726 bp
ATGAACTTGGTGAGGCTCTTGATGATCTGCCAGCCTCAAAAGGGTCCTATGGTGACAAATGTGAACACTTGCGTTGATGGGTTTGTCGAATTGGAGGAATTGGACTCGGAAAATGAAAAGGGTCTAAACCACAAAGACCAAATCCAGGGTTTAGTGACTCCTATTTCTCATATGATGAGTAATTGCTTAGCAGTGATTAAAAACAATGAAGAATTAGTACCAAGTGAGCAGAATAGGGTTATAAGTACCAAAAAGTCTTCAAAGAAAAGGCGGAGAATGCCACATTGGAAGTTGTTGCCCGGTGACTGGAATTTGGTTCAGAAAGAAGGTACAATTTGGCCTGACATTGTTGTTGCTGGTGATGGATGTGGAGAATGTGAGACTATTGGTGAAGCATTGGTGATGGCTCCTAATAATACTGTAATTGGGGACAGGTTTTTGGCTAGGGACTTGACCATTACCAACACAGCAGGTTCACAAAAGTATCAGGAGGAAGTGAGGAACTATTCAATAGTGATGAACACCCCACAAGGGTGGTCAAAGTGGGATGAGTATAGTAGAATGGACACTGTCGAGTACATTGAGTACTTGAATTTTGGACCTGGATGGCTGGATCAGACACAATGCATAGGGTCAAAGGGAGAGGGTATAGAAAGAATTGTACTGAAGAGATTGCGAAACCATTCTCCGCCAGGTTGTTCTTGCACGAAGCAGGGCAATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

241

Amino Acids

27.11

Weight (kDa)

5.88

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 461
AciI CCGC 2 cut(s) 271, 689
AclWI GGATC 1 cut(s) 621
AcsI RAATTY 2 cut(s) 310, 592
AcuI CTGAAG 1 cut(s) 686
AfaI GTAC 6 cut(s) 219, 247, 331, 578, 587, 663
AfiI CCNNNNNNNGG 4 cut(s) 41, 145, 306, 531
AgsI TTSAA 3 cut(s) 261, 510, 592
AjnI CCWGG 3 cut(s) 144, 601, 691
AjuI GAANNNNNNNTTGG 2 cut(s) 268, 300
AleI CACNNNNGTG 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 621
AoxI GGCC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 310, 592
Asp700I GAANNNNTTC 1 cut(s) 682
AspS9I GGNCC 2 cut(s) 44, 599
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 6 cut(s) 22, 64, 314, 371, 401, 412
AvaII GGWCC 2 cut(s) 44, 599
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BbsI GAAGAC 1 cut(s) 249
BccI CCATC 4 cut(s) 71, 356, 397, 600
BciT130I CCWGG 3 cut(s) 146, 603, 693
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 2 cut(s) 444, 724
BfuAI ACCTGC 1 cut(s) 461
BlpI GCTNAGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 4 cut(s) 146, 300, 603, 693
Bme18I GGWCC 2 cut(s) 44, 599
BmgT120I GGNCC 2 cut(s) 44, 599
BmiI GGNNCC 2 cut(s) 45, 408
BmrFI CCNGG 4 cut(s) 146, 300, 603, 693
BpiI GAAGAC 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 145, 306, 531
Bse1I ACTGG 1 cut(s) 311
Bse3DI GCAATG 1 cut(s) 724
BseBI CCWGG 3 cut(s) 146, 603, 693
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 3 cut(s) 371, 553, 611
BseLI CCNNNNNNNGG 4 cut(s) 41, 145, 306, 531
BseMI GCAATG 1 cut(s) 724
BseNI ACTGG 1 cut(s) 311
BshFI GGCC 1 cut(s) 340
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 2 cut(s) 443, 461
BslI CCNNNNNNNGG 4 cut(s) 41, 145, 306, 531
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 443, 461
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 340
Bsp143I GATC 2 cut(s) 24, 613
Bsp1720I GCTNAGC 1 cut(s) 187
BspACI CCGC 2 cut(s) 271, 689
BspANI GGCC 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 45, 408
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 1 cut(s) 621
BsrDI GCAATG 1 cut(s) 724
BsrI ACTGG 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 2 cut(s) 24, 613
Bst2UI CCWGG 3 cut(s) 146, 603, 693
Bst4CI ACNGT 2 cut(s) 421, 571
Bst6I CTCTTC 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 187
BstF5I GGATG 3 cut(s) 371, 553, 611
BstKTI GATC 2 cut(s) 27, 616
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 2 cut(s) 24, 613
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNI CCWGG 3 cut(s) 146, 603, 693
BstSCI CCNGG 4 cut(s) 144, 298, 601, 691
BstV2I GAAGAC 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 534
BsuRI GGCC 1 cut(s) 340
BtsCI GGATG 3 cut(s) 371, 553, 611
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 567
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 44, 599
Csp6I GTAC 6 cut(s) 218, 246, 330, 577, 586, 662
CviJI RGCY 7 cut(s) 16, 34, 340, 407, 443, 610, 723
CviKI_1 RGCY 7 cut(s) 16, 34, 340, 407, 443, 610, 723
CviQI GTAC 6 cut(s) 218, 246, 330, 577, 586, 662
DdeI CTNAG 1 cut(s) 187
DpnI GATC 2 cut(s) 26, 615
DpnII GATC 2 cut(s) 24, 613
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
EciI GGCGGA 2 cut(s) 286, 678
Eco47I GGWCC 2 cut(s) 44, 599
Eco57I CTGAAG 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 144, 601, 691
EcoT22I ATGCAT 1 cut(s) 629
FaiI YATR 7 cut(s) 50, 171, 173, 242, 555, 629, 650
FaqI GGGAC 2 cut(s) 443, 461
FauNDI CATATG 1 cut(s) 171
FokI GGATG 3 cut(s) 378, 560, 618
FspBI CTAG 2 cut(s) 444, 724
HaeIII GGCC 1 cut(s) 340
HapII CCGG 1 cut(s) 300
HinfI GANTC 2 cut(s) 104, 157
HpaII CCGG 1 cut(s) 300
HphI GGTGA 6 cut(s) 22, 64, 314, 371, 401, 412
Hpy166II GTNNAC 2 cut(s) 64, 476
Hpy188I TCNGA 3 cut(s) 109, 321, 618
Hpy188III TCNNGA 2 cut(s) 19, 488
Hpy8I GTNNAC 2 cut(s) 64, 476
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 2 cut(s) 421, 571
HpyCH4V TGCA 2 cut(s) 627, 705
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 24, 613
LmnI GCTCC 1 cut(s) 412
MaeI CTAG 2 cut(s) 444, 724
MaeIII GTNAC 3 cut(s) 52, 154, 302
MalI GATC 2 cut(s) 26, 615
MboI GATC 2 cut(s) 24, 613
MboII GAAGA 3 cut(s) 221, 249, 679
MluCI AATT 9 cut(s) 89, 98, 181, 212, 310, 333, 423, 592, 657
MlyI GAGTC 2 cut(s) 98, 151
MnlI CCTC 6 cut(s) 6, 45, 88, 484, 492, 637
Mph1103I ATGCAT 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 1 cut(s) 198
MslI CAYNNNNRTG 1 cut(s) 532
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 4 cut(s) 146, 300, 603, 693
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 3 cut(s) 146, 603, 693
MwoI GCNNNNNNNGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 171
NdeII GATC 2 cut(s) 24, 613
NlaIV GGNNCC 2 cut(s) 45, 408
NmuCI GTSAC 3 cut(s) 52, 154, 302
NsiI ATGCAT 1 cut(s) 629
OliI CACNNNNGTG 1 cut(s) 532
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 682
PflFI GACNNNGTC 1 cut(s) 569
PleI GAGTC 2 cut(s) 98, 151
PpsI GAGTC 2 cut(s) 98, 151
PpuMI RGGWCCY 1 cut(s) 44
PsiI TTATAA 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 3 cut(s) 144, 601, 691
PspGI CCWGG 3 cut(s) 144, 601, 691
PspN4I GGNNCC 2 cut(s) 45, 408
PspPI GGNCC 2 cut(s) 44, 599
PspPPI RGGWCCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 569
RsaI GTAC 6 cut(s) 219, 247, 331, 578, 587, 663
RsaNI GTAC 6 cut(s) 218, 246, 330, 577, 586, 662
RseI CAYNNNNRTG 1 cut(s) 532
SaqAI TTAA 1 cut(s) 198
Sau3AI GATC 2 cut(s) 24, 613
Sau96I GGNCC 2 cut(s) 44, 599
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 2 cut(s) 98, 151
ScrFI CCNGG 4 cut(s) 146, 300, 603, 693
SetI ASST 5 cut(s) 331, 437, 475, 604, 697
SinI GGWCC 2 cut(s) 44, 599
SmiMI CAYNNNNRTG 1 cut(s) 532
Sse9I AATT 9 cut(s) 89, 98, 181, 212, 310, 333, 423, 592, 657
SsiI CCGC 2 cut(s) 271, 689
SspMI CTAG 2 cut(s) 444, 724
StyD4I CCNGG 4 cut(s) 144, 298, 601, 691
TaaI ACNGT 2 cut(s) 421, 571
TaqI TCGA 2 cut(s) 87, 573
TasI AATT 9 cut(s) 89, 98, 181, 212, 310, 333, 423, 592, 657
TatI WGTACW 3 cut(s) 576, 585, 661
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 2 cut(s) 198, 574
TseFI GTSAC 3 cut(s) 52, 154, 302
Tsp45I GTSAC 3 cut(s) 52, 154, 302
TspDTI ATGAA 4 cut(s) 17, 129, 222, 533
TspRI CASTG 2 cut(s) 198, 574
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 44, 599
XapI RAATTY 2 cut(s) 310, 592
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 2 cut(s) 444, 724
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.