Rh2DG104400

Pectinesterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0017963
Physical Location & Seq
Reverse (-)
8537661 .. 8538053
393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG104400.1

Sequence Viewer

Length: 393 bp
ATGCGCTCAAAGATTGCCTGTAGATGTTTGATCAAACACTCTAAGAACTTGGTGAGGCTCTTGATGATCTGCCAGCCTCAAAAGGGTCCTATGGTGACAAATGTGAACACTTGCGTTTTCGAATTGGAGGAATTGGACTGGAAAATGAAAAGGCTAAACTTCAAAGACCAAATCCAGGGTTTAGTGACTCCTATTTCTCATATGATGAGTAATTGCTTAGCAATGATTAAAAACATTGAAGAATTAGTACTAAGTGAGCAGAAGAGGGTTATAAGTACCGAGAAGTCTTCAAAGAAAAGAAGGCGAGTGGAAGTTACATTCTTTGCAGCTGTAATTGGGGACAGGTTTTTGGCTAGGGACTTGACCATTACCAACACAGCATGTTCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

130

Amino Acids

14.99

Weight (kDa)

9.69

Isoelectric Point (pI)

54.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017963)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09320
malus_domestica MD02G1100400.v1.1 MD15G1223400.v1.1
prunus_persica Prupe.7G192400_v2.0.a1
rosa_laevigata RLG00000016604
rosa_roxburghii Rroxscaffold_2G00145990
rosa_rugosa Rorug02G0053600
rosa_samantha Rh2AG102800 Rh2BG104200 Rh2CG106700 Rh2DG104400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AfaI GTAC 2 cut(s) 249, 277
AfiI CCNNNNNNNGG 2 cut(s) 83, 175
AgsI TTSAA 3 cut(s) 163, 239, 291
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 326
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 64, 106
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 86
BarI GAAGNNNNNNTAC 2 cut(s) 231, 263
BbsI GAAGAC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 338
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 1 cut(s) 30
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 19
BisI GCNGC 1 cut(s) 327
BlpI GCTNAGC 1 cut(s) 217
BlsI GCNGC 1 cut(s) 328
BmcAI AGTACT 1 cut(s) 249
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 176
BpiI GAAGAC 1 cut(s) 279
Bpu1102I GCTNAGC 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 175
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 83, 175
BseMI GCAATG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 338
BslFI GGGAC 2 cut(s) 353, 371
BslI CCNNNNNNNGG 2 cut(s) 83, 175
BsmFI GGGAC 2 cut(s) 353, 371
Bsp119I TTCGAA 1 cut(s) 120
Bsp143I GATC 2 cut(s) 30, 66
Bsp1720I GCTNAGC 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 87
BspT104I TTCGAA 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 2 cut(s) 30, 66
Bst2UI CCWGG 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 3 cut(s) 42, 217, 251
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 2 cut(s) 33, 69
BstMBI GATC 2 cut(s) 30, 66
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 74
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 248, 276
CviAII CATG 1 cut(s) 381
CviJI RGCY 5 cut(s) 58, 76, 154, 329, 353
CviKI_1 RGCY 5 cut(s) 58, 76, 154, 329, 353
CviQI GTAC 2 cut(s) 248, 276
DdeI CTNAG 3 cut(s) 42, 217, 251
DpnI GATC 2 cut(s) 32, 68
DpnII GATC 2 cut(s) 30, 66
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 174
FaeI CATG 1 cut(s) 384
FaiI YATR 5 cut(s) 92, 201, 203, 272, 382
FaqI GGGAC 2 cut(s) 353, 371
FatI CATG 1 cut(s) 380
FauNDI CATATG 1 cut(s) 201
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 327
HhaI GCGC 1 cut(s) 6
Hin1II CATG 1 cut(s) 384
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 1 cut(s) 187
HphI GGTGA 2 cut(s) 64, 106
Hpy166II GTNNAC 2 cut(s) 106, 386
Hpy188III TCNNGA 1 cut(s) 61
Hpy8I GTNNAC 2 cut(s) 106, 386
HpyAV CCTTC 1 cut(s) 294
HpyCH4V TGCA 1 cut(s) 326
HpyF3I CTNAG 3 cut(s) 42, 217, 251
Hsp92II CATG 1 cut(s) 384
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 2 cut(s) 30, 66
LpnPI CCDG 6 cut(s) 31, 86, 124, 161, 188, 328
Lsp1109I GCAGC 1 cut(s) 338
MaeI CTAG 1 cut(s) 354
MaeIII GTNAC 3 cut(s) 94, 184, 313
MalI GATC 2 cut(s) 32, 68
MboI GATC 2 cut(s) 30, 66
MboII GAAGA 3 cut(s) 251, 274, 279
MluCI AATT 5 cut(s) 122, 131, 211, 242, 333
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 4 cut(s) 48, 87, 121, 258
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 176
MvaI CCWGG 1 cut(s) 176
NdeI CATATG 1 cut(s) 201
NdeII GATC 2 cut(s) 30, 66
NlaIII CATG 1 cut(s) 384
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 94, 184
NspI RCATGY 1 cut(s) 384
NspV TTCGAA 1 cut(s) 120
PkrI GCNGC 1 cut(s) 328
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PpuMI RGGWCCY 1 cut(s) 86
PsiI TTATAA 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 86
PspPPI RGGWCCY 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 249, 277
RsaNI GTAC 2 cut(s) 248, 276
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 2 cut(s) 30, 66
Sau96I GGNCC 1 cut(s) 86
ScaI AGTACT 1 cut(s) 249
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 176
SetI ASST 2 cut(s) 331, 347
SfcI CTRYAG 1 cut(s) 19
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 86
Sse9I AATT 5 cut(s) 122, 131, 211, 242, 333
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 174
TaqI TCGA 1 cut(s) 120
TasI AATT 5 cut(s) 122, 131, 211, 242, 333
TatI WGTACW 1 cut(s) 247
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 2 cut(s) 94, 184
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 2 cut(s) 94, 184
TspDTI ATGAA 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 86
XceI RCATGY 1 cut(s) 384
XspI CTAG 1 cut(s) 354
ZrmI AGTACT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.