Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0004382

Physical Location & Seq

Genomic Coordinates Forward (+)

8239997 .. 8244699

Gene Structure 4703 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g14740.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGCTCCACCTCGTAAAACCCCTCCTCCGCCCCCATCTCCGCCGCATGCGGTGGGCCCACTCCAAGGCCCCAAAGCCCCCCAAACCGCCGCAAGCCCCTCCCAAACCCCCCAACCCGCCGCAAAAACCCGAAGCCTTCACCCTCCACGACCACACGTGGCACGACCCCTACAGCTGGATGTCCAAGCTCAACGACAAGGTCGCCATGCGACACATGGACATGTACATGGAACAGGAAGACAACTACACCCAAGCTGTCATGTCCGGCACCGACCGCCTCCAAAGTAAGCTCCAGTCCGAGATGGCCTCCCGCTTATCCTTCGACCTCTCCACTCCTCCCCTCAAATTCGGACCATGGTTGTACTACCGGCGCGTGGAGGAAGGGAAGCAGTTTCCGGTGCTGTGTCGGCGACTCGCGAGTTTACAAGACGAGTTCATTTCTCATAACTTGCCGTCTGCTGGGTTCGATTTCACTTCCGGGAAGAGAATTGAGCAGAAGCTCATTGATTACAATCTAGAAGCTCAAAGATTTGGAGGCTATGCATATGAAGAGTTATCTGAAGTTTCGCCGGATCATCGGTTTATTGCTTATACAATGTATGATAAAGACAAGGACTTGTTTAGGTTGTCTGTTAGGAACTTGAATTCTGGTGCATTGTGTAGTAAGCCTCAAGCGGAGCGTGTTTCGAATTTGGCTTGGGCCAAAGACGGGCAGGCGCTGCTTTATTGTGTTACTGATCAGAACAAGCGGCCGTATAGGATATATTGTAGCATGATTGGATCGACTGATGAGGACGTTTTGGTTTTTGAAGAATCGGATAATGATGTGTTTGTCAACATTAGACACACCAAGGATTTCCGGTTTGTGACTGTAAATACCTTCTCGATTGAGTCTTCCAAGGTCTTTTTGATAAATGCAGCTGATCCACTGTCGGGGATGGACTTAGTTTGGCAGTGTGAAGGTATAGCGCATTGCATAATTGAGCATCACCAAGGCTGTCTTTTTCTTTTTACAGATGCTGCCAAAGGAGGGCAGTCAGTTGATAACCACTATCTCCTTCGTAGTCCTGTGGATTCTTCTAGCAGTCCAAGAATATGGGAGGATGTATTTGTCAGTGACCCAGACTGGGTTGTTGAGGACATGGATTTCAGTCATACACACTTGGTACTTAATATAAGGGAAGGGCGAAACTTTAGACTTTGTTCTGTTGCTTTACCTTTGCCTGCTGGGAAGGGACCAGTTCATCTAAAAGAGCTTCATCCACAATTCTTGCCTCTTCCAAAATATGTTTCACAAATTTCACCTGGGCCAAATTATGACTACCATTCATCAAATATGCGCTTTACAATATCATCACCTGTGATGCCAGATGCTGTGGTTGATTATGACCTGTCAAATGGAAACTGGAAGATCATTCAACAGCAGAATATTCTCCATGAAAGAACAAAGGTTTTGTATGGAACAGCTTCTTCAGCAAGCATCAGTGAGAACACCTTAAATACAGATGTTTTCAATTCTGCAAATGAAGTCAGCCCTGATGATCAGCAGTTGTGGAATGATCTTTCTGAATTTTATGCTTGTGAACAGCATAATGTCTCTTCTTATGAGGGAGTCTTGGTTCCATTAACTGTTGTGTACTCTCGCAAAACCAAGAAAGAGGATCAGAACCCTGGATTGCTTCATGTGCATGGAGCTTATGGTGAGCTACTCGATAAAAGGTGGCGCAGTGAATTGAAAAGCCTTCTTGATCGTGGTTGGATAGTTGCATATGCTGATCTTAGAGGTGGAGGCGGTGGAGGTAAGAAGTGGCATTATGATGGAAGACGTACAAAGAAGATAAATTCCATCAAGGATTTTATATCATGTGCCAATTTCCTTGTTGACAAAGAAATTGTCCATGAAAAGAAACTTGCTGCATCGGGTTATAGTGCTGGAGGACTTGTAGTTGCTTCAGCCGTCAATCAATGCCCACATTTGTTTACTGCTGCAGTTTTGAAGGTTCCATTTCTAGATGCAACAAGCACTCTTCTCTATCCCATTATACCGCTCACAGCTGCTGATTATGAAGAGTTTGGATACCCCGGGGACATTAATGATTTTCATGCTATACGCGATTACTCTCCATATGACAATATTCAGAAGGGTGTTCTCTATCCAGCTGTACTGATTTCCTCATCTTTCAATACACGTTTTGGAGTGTGGGAAGCTGCAAAATGGGTTGCACGAGTTCGTGAACGTACAATTTACGACCCAAAGCGTCCAATACTGCTTAATTTGACAACAGACATAGTAGAGGAAAACAGGTACCTCCAATGCAAGGAATCGGCACTAGAGACTGCATTTCTTATAAAGGCAATGGAATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0070011 GO:0070012 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S9 Peptidase_S9_N

Protein Analysis

789

Amino Acids

89.67

Weight (kDa)

6.27

Isoelectric Point (pI)

50.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S9_N	PF02897	29 - 277	2e-16	Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9	PF00326	378 - 548	6.2e-35	Prolyl oligopeptidase family
Hydrolase_4	PF12146	381 - 478	9.1e-06	Serine aminopeptidase, S33

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66960
fragaria_vesca	FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740
malus_domestica	MD15G1274800.v1.1
prunus_persica	Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1
pyrus_communis	pycom15g23790
rosa_chinensis	RchiOBHm_Chr1g0319111 RchiOBHm_Chr2g0103681
rosa_laevigata	RLG00000017248 RLG00000029580 RLG00000030483
rosa_multiflora	Rmu_sc0001852.1_g000011 Rmu_sc0003574.1_g000010 Rmu_sc0009469.1_g000007
rosa_roxburghii	Rroxscaffold_2G00139490 Rroxscaffold_4G00328470
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh1AG038700 Rh2AG165200 Rh2BG172400 Rh2CG171300 Rh2DG170800 Rh5CG361300
rosa_wichuraiana	Rw1G003040 Rw2G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2348
Acc65I	GGTACC	1 cut(s)	2304
AccB1I	GGYRCC	2 cut(s)	266, 2304
AccBSI	CCGCTC	1 cut(s)	2047
AccII	CGCG	3 cut(s)	372, 416, 2112
AclWI	GGATC	4 cut(s)	579, 787, 917, 1668
AcoI	YGGCCR	1 cut(s)	749
AcsI	RAATTY	6 cut(s)	344, 643, 688, 1296, 1568, 1840
AcuI	CTGAAG	3 cut(s)	579, 1455, 1935
AcvI	CACGTG	1 cut(s)	156
AfaI	GTAC	8 cut(s)	224, 362, 1167, 1637, 1828, 2163, 2239, 2306
AfiI	CCNNNNNNNGG	9 cut(s)	64, 174, 373, 458, 708, 932, 1029, 1126, 2317
AflIII	ACRYGT	3 cut(s)	153, 219, 2186
AgsI	TTSAA	7 cut(s)	643, 809, 1418, 1513, 1735, 1996, 2182
AjnI	CCWGG	2 cut(s)	1303, 1669
AjuI	GAANNNNNNNTTGG	2 cut(s)	1273, 1305
AloI	GAACNNNNNNTCC	2 cut(s)	1602, 1634
Alw26I	GTCTC	2 cut(s)	1600, 2327
AlwI	GGATC	4 cut(s)	579, 787, 917, 1668
AlwNI	CAGNNNCTG	4 cut(s)	718, 1019, 1373, 2057
Ama87I	CYCGRG	1 cut(s)	2081
AoxI	GGCC	6 cut(s)	54, 66, 303, 699, 749, 1307
ApaI	GGGCCC	1 cut(s)	58
ApeKI	GCWGC	7 cut(s)	718, 917, 1019, 1913, 1985, 2054, 2207
ApoI	RAATTY	6 cut(s)	344, 643, 688, 1296, 1568, 1840
ArsI	GACNNNNNNTTYG	2 cut(s)	1878, 1910
AseI	ATTAAT	1 cut(s)	2091
Asp700I	GAANNNNTTC	1 cut(s)	1465
Asp718I	GGTACC	1 cut(s)	2304
AspLEI	GCGC	5 cut(s)	372, 718, 970, 1341, 1725
AspS9I	GGNCC	7 cut(s)	54, 55, 67, 350, 699, 1235, 1307
AsuC2I	CCSGG	3 cut(s)	478, 2082, 2083
AsuHPI	GGTGA	5 cut(s)	130, 980, 1293, 1347, 1712
AsuII	TTCGAA	1 cut(s)	686
AvaI	CYCGRG	1 cut(s)	2081
AvaII	GGWCC	2 cut(s)	350, 1235
BaeGI	GKGCMC	1 cut(s)	58
BanI	GGYRCC	2 cut(s)	266, 2304
BanII	GRGCYC	1 cut(s)	58
BauI	CACGAG	1 cut(s)	2223
BbrPI	CACGTG	1 cut(s)	156
BbsI	GAAGAC	3 cut(s)	243, 885, 1828
BbvI	GCAGC	7 cut(s)	705, 929, 1006, 1900, 1972, 2041, 2194
BccI	CCATC	5 cut(s)	42, 295, 931, 1811, 1853
BceAI	ACGGC	3 cut(s)	436, 736, 1940
BcgI	CGANNNNNNTGC	1 cut(s)	2343
BciT130I	CCWGG	2 cut(s)	1305, 1671
BciVI	GTATCC	1 cut(s)	2069
BclI	TGATCA	2 cut(s)	736, 1540
BcnI	CCSGG	3 cut(s)	478, 2082, 2083
BcoDI	GTCTC	2 cut(s)	1600, 2327
BfaI	CTAG	4 cut(s)	515, 1080, 2009, 2330
BfmI	CTRYAG	2 cut(s)	169, 1986
BfoI	RGCGCY	1 cut(s)	719
BfuI	GTATCC	1 cut(s)	2069
Bme1390I	CCNGG	5 cut(s)	478, 1305, 1671, 2082, 2083
Bme18I	GGWCC	2 cut(s)	350, 1235
BmeT110I	CYCGRG	1 cut(s)	2081
BmgT120I	GGNCC	7 cut(s)	54, 55, 67, 350, 699, 1235, 1307
BmiI	GGNNCC	7 cut(s)	56, 69, 268, 1236, 1620, 2001, 2306
BmrFI	CCNGG	5 cut(s)	478, 1305, 1671, 2082, 2083
BmrI	ACTGGG	1 cut(s)	1135
BmsI	GCATC	7 cut(s)	994, 1006, 1353, 1360, 1488, 1925, 2002
BmuI	ACTGGG	1 cut(s)	1135
BpiI	GAAGAC	3 cut(s)	243, 885, 1828
BplI	GAGNNNNNCTC	2 cut(s)	290, 322
BpmI	CTGGAG	2 cut(s)	275, 1953
Bpu14I	TTCGAA	1 cut(s)	686
BpuEI	CTTGAG	1 cut(s)	654
BpuMI	CCSGG	3 cut(s)	478, 2082, 2083
BsaAI	YACGTR	1 cut(s)	156
BsaBI	GATNNNNATC	1 cut(s)	510
BsaWI	WCCGGW	2 cut(s)	394, 858
BsaXI	ACNNNNNCTCC	6 cut(s)	9, 39, 1602, 1632, 1926, 1956
Bsc4I	CCNNNNNNNGG	9 cut(s)	64, 174, 373, 458, 708, 932, 1029, 1126, 2317
Bse118I	RCCGGY	1 cut(s)	366
Bse1I	ACTGG	4 cut(s)	292, 1130, 1238, 1409
Bse3DI	GCAATG	2 cut(s)	970, 2361
Bse8I	GATNNNNATC	1 cut(s)	510
BseBI	CCWGG	2 cut(s)	1305, 1671
BseGI	GGATG	4 cut(s)	183, 942, 1108, 1258
BseJI	GATNNNNATC	1 cut(s)	510
BseLI	CCNNNNNNNGG	9 cut(s)	64, 174, 373, 458, 708, 932, 1029, 1126, 2317
BseMI	GCAATG	2 cut(s)	970, 2361
BseNI	ACTGG	4 cut(s)	292, 1130, 1238, 1409
BseRI	GAGGAG	2 cut(s)	14, 324
BseSI	GKGCMC	1 cut(s)	58
BseX3I	CGGCCG	1 cut(s)	749
BseXI	GCAGC	7 cut(s)	705, 929, 1006, 1900, 1972, 2041, 2194
BseYI	CCCAGC	2 cut(s)	458, 1226
Bsh1236I	CGCG	3 cut(s)	372, 416, 2112
Bsh1285I	CGRYCG	2 cut(s)	274, 752
BshFI	GGCC	6 cut(s)	56, 68, 305, 701, 751, 1309
BshNI	GGYRCC	2 cut(s)	266, 2304
BsiEI	CGRYCG	2 cut(s)	274, 752
BsiHKCI	CYCGRG	1 cut(s)	2081
BsiSI	CCGG	7 cut(s)	264, 367, 395, 477, 569, 859, 2082
BslFI	GGGAC	2 cut(s)	1248, 2099
BslI	CCNNNNNNNGG	9 cut(s)	64, 174, 373, 458, 708, 932, 1029, 1126, 2317
BsmAI	GTCTC	2 cut(s)	1600, 2327
BsmFI	GGGAC	2 cut(s)	1248, 2099
BsnI	GGCC	6 cut(s)	56, 68, 305, 701, 751, 1309
BsoBI	CYCGRG	1 cut(s)	2081
Bsp119I	TTCGAA	1 cut(s)	686
Bsp120I	GGGCCC	1 cut(s)	54
Bsp1286I	GDGCHC	1 cut(s)	58
Bsp1407I	TGTACA	1 cut(s)	222
Bsp19I	CCATGG	1 cut(s)	353
Bsp68I	TCGCGA	1 cut(s)	416
BspANI	GGCC	6 cut(s)	56, 68, 305, 701, 751, 1309
BspFNI	CGCG	3 cut(s)	372, 416, 2112
BspLI	GGNNCC	7 cut(s)	56, 69, 268, 1236, 1620, 2001, 2306
BspMAI	CTGCAG	1 cut(s)	1990
BspPI	GGATC	4 cut(s)	579, 787, 917, 1668
BspT104I	TTCGAA	1 cut(s)	686
BspT107I	GGYRCC	2 cut(s)	266, 2304
BsrBI	CCGCTC	1 cut(s)	2047
BsrDI	GCAATG	2 cut(s)	970, 2361
BsrFI	RCCGGY	1 cut(s)	366
BsrGI	TGTACA	1 cut(s)	222
BsrI	ACTGG	4 cut(s)	292, 1130, 1238, 1409
BssAI	RCCGGY	1 cut(s)	366
BssSI	CACGAG	1 cut(s)	2223
BssT1I	CCWWGG	5 cut(s)	63, 353, 849, 897, 991
Bst2BI	CACGAG	1 cut(s)	2223
Bst2UI	CCWGG	2 cut(s)	1305, 1671
Bst4CI	ACNGT	3 cut(s)	871, 930, 1630
Bst6I	CTCTTC	6 cut(s)	476, 543, 1281, 1603, 2031, 2061
BstAPI	GCANNNNNTGC	1 cut(s)	718
BstAUI	TGTACA	1 cut(s)	222
BstBAI	YACGTR	1 cut(s)	156
BstBI	TTCGAA	1 cut(s)	686
BstC8I	GCNNGC	5 cut(s)	47, 93, 714, 1224, 1477
BstDEI	CTNAG	2 cut(s)	943, 1778
BstDSI	CCRYGG	1 cut(s)	353
BstF5I	GGATG	4 cut(s)	183, 942, 1108, 1258
BstFNI	CGCG	3 cut(s)	372, 416, 2112
BstH2I	RGCGCY	1 cut(s)	719
BstHHI	GCGC	5 cut(s)	372, 718, 970, 1341, 1725
BstMAI	GTCTC	2 cut(s)	1600, 2327
BstMCI	CGRYCG	2 cut(s)	274, 752
BstMWI	GCNNNNNNNGC	5 cut(s)	273, 406, 718, 1472, 1684
BstNI	CCWGG	2 cut(s)	1305, 1671
BstNSI	RCATGY	2 cut(s)	49, 223
BstSCI	CCNGG	5 cut(s)	476, 1303, 1669, 2080, 2081
BstSFI	CTRYAG	2 cut(s)	169, 1986
BstSLI	GKGCMC	1 cut(s)	58
BstUI	CGCG	3 cut(s)	372, 416, 2112
BstV1I	GCAGC	7 cut(s)	705, 929, 1006, 1900, 1972, 2041, 2194
BstV2I	GAAGAC	3 cut(s)	243, 885, 1828
BstXI	CCANNNNNNTGG	1 cut(s)	1095
BstZI	CGGCCG	1 cut(s)	749
BsuI	GTATCC	1 cut(s)	2069
BsuRI	GGCC	6 cut(s)	56, 68, 305, 701, 751, 1309
BtgI	CCRYGG	1 cut(s)	353
BtsCI	GGATG	4 cut(s)	183, 942, 1108, 1258
BtsI	GCAGTG	2 cut(s)	959, 1732
BtsIMutI	CAGTG	5 cut(s)	926, 959, 1120, 1489, 1732
BtuMI	TCGCGA	1 cut(s)	416
Cac8I	GCNNGC	5 cut(s)	47, 93, 714, 1224, 1477
CaiI	CAGNNNCTG	4 cut(s)	718, 1019, 1373, 2057
CfoI	GCGC	5 cut(s)	372, 718, 970, 1341, 1725
Cfr10I	RCCGGY	1 cut(s)	366
Cfr13I	GGNCC	7 cut(s)	54, 55, 67, 350, 699, 1235, 1307
Cfr9I	CCCGGG	1 cut(s)	2081
CseI	GACGC	1 cut(s)	2246
Csp6I	GTAC	8 cut(s)	223, 361, 1166, 1636, 1827, 2162, 2238, 2305
CspCI	CAANNNNNGTGG	2 cut(s)	1251, 1286
CviQI	GTAC	8 cut(s)	223, 361, 1166, 1636, 1827, 2162, 2238, 2305
DdeI	CTNAG	2 cut(s)	943, 1778
EaeI	YGGCCR	1 cut(s)	749
EagI	CGGCCG	1 cut(s)	749
Eam1104I	CTCTTC	6 cut(s)	476, 543, 1281, 1603, 2031, 2061
EarI	CTCTTC	6 cut(s)	476, 543, 1281, 1603, 2031, 2061
EciI	GGCGGA	2 cut(s)	17, 29
EclXI	CGGCCG	1 cut(s)	749
Eco130I	CCWWGG	5 cut(s)	63, 353, 849, 897, 991
Eco24I	GRGCYC	1 cut(s)	58
Eco47I	GGWCC	2 cut(s)	350, 1235
Eco52I	CGGCCG	1 cut(s)	749
Eco57I	CTGAAG	3 cut(s)	579, 1455, 1935
Eco72I	CACGTG	1 cut(s)	156
Eco88I	CYCGRG	1 cut(s)	2081
EcoO109I	RGGNCCY	1 cut(s)	67
EcoRI	GAATTC	1 cut(s)	643
EcoRII	CCWGG	2 cut(s)	1303, 1669
EcoT14I	CCWWGG	5 cut(s)	63, 353, 849, 897, 991
EcoT22I	ATGCAT	1 cut(s)	544
EcoT38I	GRGCYC	1 cut(s)	58
ErhI	CCWWGG	5 cut(s)	63, 353, 849, 897, 991
FalI	AAGNNNNNCTT	2 cut(s)	984, 1016
FaqI	GGGAC	2 cut(s)	1248, 2099
FauI	CCCGC	2 cut(s)	123, 317
FauNDI	CATATG	3 cut(s)	544, 1768, 2125
FbaI	TGATCA	2 cut(s)	736, 1540
FokI	GGATG	4 cut(s)	190, 949, 1115, 1245
FriOI	GRGCYC	1 cut(s)	58
FspBI	CTAG	4 cut(s)	515, 1080, 2009, 2330
GlaI	GCGC	5 cut(s)	371, 717, 969, 1340, 1724
GsaI	CCCAGC	2 cut(s)	462, 1230
GsuI	CTGGAG	2 cut(s)	275, 1953
HaeII	RGCGCY	1 cut(s)	719
HaeIII	GGCC	6 cut(s)	56, 68, 305, 701, 751, 1309
HapII	CCGG	7 cut(s)	264, 367, 395, 477, 569, 859, 2082
HgaI	GACGC	1 cut(s)	2246
HhaI	GCGC	5 cut(s)	372, 718, 970, 1341, 1725
Hin6I	GCGC	5 cut(s)	370, 716, 968, 1339, 1723
HinP1I	GCGC	5 cut(s)	370, 716, 968, 1339, 1723
HincII	GTYRAC	2 cut(s)	835, 1882
HindII	GTYRAC	2 cut(s)	835, 1882
HinfI	GANTC	7 cut(s)	411, 812, 890, 1073, 1611, 2321, 2360
HpaII	CCGG	7 cut(s)	264, 367, 395, 477, 569, 859, 2082
HphI	GGTGA	5 cut(s)	130, 980, 1293, 1347, 1712
Hpy166II	GTNNAC	7 cut(s)	422, 835, 1583, 1636, 1882, 1980, 2234
Hpy188I	TCNGA	8 cut(s)	298, 350, 559, 741, 817, 1567, 1665, 2139
Hpy188III	TCNNGA	6 cut(s)	415, 515, 883, 1745, 2009, 2231
Hpy8I	GTNNAC	7 cut(s)	422, 835, 1583, 1636, 1882, 1980, 2234
HpyCH4III	ACNGT	3 cut(s)	871, 930, 1630
HpyCH4IV	ACGT	5 cut(s)	155, 795, 1825, 2188, 2236
HpyF10VI	GCNNNNNNNGC	5 cut(s)	273, 406, 718, 1472, 1684
HpyF3I	CTNAG	2 cut(s)	943, 1778
HpySE526I	ACGT	5 cut(s)	155, 795, 1825, 2188, 2236
HspAI	GCGC	5 cut(s)	370, 716, 968, 1339, 1723
KpnI	GGTACC	1 cut(s)	2308
Ksp22I	TGATCA	2 cut(s)	736, 1540
LmnI	GCTCC	4 cut(s)	9, 294, 676, 1691
Lsp1109I	GCAGC	7 cut(s)	705, 929, 1006, 1900, 1972, 2041, 2194
LweI	GCATC	7 cut(s)	994, 1006, 1353, 1360, 1488, 1925, 2002
MaeI	CTAG	4 cut(s)	515, 1080, 2009, 2330
MaeII	ACGT	5 cut(s)	155, 795, 1825, 2188, 2236
MaeIII	GTNAC	3 cut(s)	730, 865, 1115
MbiI	CCGCTC	1 cut(s)	2047
MhlI	GDGCHC	1 cut(s)	58
MlyI	GAGTC	3 cut(s)	405, 899, 1620
MmeI	TCCRAC	1 cut(s)	1736
Mph1103I	ATGCAT	1 cut(s)	544
MroXI	GAANNNNTTC	1 cut(s)	1465
MseI	TTAA	5 cut(s)	1170, 1496, 1625, 2091, 2271
MslI	CAYNNNNRTG	4 cut(s)	218, 224, 1686, 1815
MspA1I	CMGCKG	4 cut(s)	174, 920, 2054, 2159
MspI	CCGG	7 cut(s)	264, 367, 395, 477, 569, 859, 2082
MspR9I	CCNGG	5 cut(s)	478, 1305, 1671, 2082, 2083
MvaI	CCWGG	2 cut(s)	1305, 1671
MvnI	CGCG	3 cut(s)	372, 416, 2112
MwoI	GCNNNNNNNGC	5 cut(s)	273, 406, 718, 1472, 1684
NciI	CCSGG	3 cut(s)	478, 2082, 2083
NcoI	CCATGG	1 cut(s)	353
NdeI	CATATG	3 cut(s)	544, 1768, 2125
NlaIV	GGNNCC	7 cut(s)	56, 69, 268, 1236, 1620, 2001, 2306
NmuCI	GTSAC	2 cut(s)	865, 1115
NruI	TCGCGA	1 cut(s)	416
NsiI	ATGCAT	1 cut(s)	544
NspI	RCATGY	2 cut(s)	49, 223
NspV	TTCGAA	1 cut(s)	686
PaeI	GCATGC	1 cut(s)	49
PciI	ACATGT	1 cut(s)	219
PdmI	GAANNNNTTC	1 cut(s)	1465
PfeI	GAWTC	4 cut(s)	812, 1073, 2321, 2360
PflFI	GACNNNGTC	1 cut(s)	197
PfoI	TCCNGGA	1 cut(s)	476
PleI	GAGTC	3 cut(s)	405, 898, 1619
PmaCI	CACGTG	1 cut(s)	156
PmlI	CACGTG	1 cut(s)	156
PpsI	GAGTC	3 cut(s)	405, 898, 1619
Ppu21I	YACGTR	1 cut(s)	156
PscI	ACATGT	1 cut(s)	219
PshBI	ATTAAT	1 cut(s)	2091
PsiI	TTATAA	1 cut(s)	2348
Psp6I	CCWGG	2 cut(s)	1303, 1669
PspCI	CACGTG	1 cut(s)	156
PspFI	CCCAGC	2 cut(s)	458, 1226
PspGI	CCWGG	2 cut(s)	1303, 1669
PspN4I	GGNNCC	7 cut(s)	56, 69, 268, 1236, 1620, 2001, 2306
PspOMI	GGGCCC	1 cut(s)	54
PspPI	GGNCC	7 cut(s)	54, 55, 67, 350, 699, 1235, 1307
PstI	CTGCAG	1 cut(s)	1990
PstNI	CAGNNNCTG	4 cut(s)	718, 1019, 1373, 2057
PsyI	GACNNNGTC	1 cut(s)	197
PvuII	CAGCTG	4 cut(s)	174, 920, 2054, 2159
RruI	TCGCGA	1 cut(s)	416
RsaI	GTAC	8 cut(s)	224, 362, 1167, 1637, 1828, 2163, 2239, 2306
RsaNI	GTAC	8 cut(s)	223, 361, 1166, 1636, 1827, 2162, 2238, 2305
RseI	CAYNNNNRTG	4 cut(s)	218, 224, 1686, 1815
SaqAI	TTAA	5 cut(s)	1170, 1496, 1625, 2091, 2271
Sau96I	GGNCC	7 cut(s)	54, 55, 67, 350, 699, 1235, 1307
SchI	GAGTC	3 cut(s)	405, 899, 1620
ScrFI	CCNGG	5 cut(s)	478, 1305, 1671, 2082, 2083
SduI	GDGCHC	1 cut(s)	58
SfaNI	GCATC	7 cut(s)	994, 1006, 1353, 1360, 1488, 1925, 2002
SfcI	CTRYAG	2 cut(s)	169, 1986
SfuI	TTCGAA	1 cut(s)	686
SinI	GGWCC	2 cut(s)	350, 1235
SmaI	CCCGGG	1 cut(s)	2083
SmiMI	CAYNNNNRTG	4 cut(s)	218, 224, 1686, 1815
SmlI	CTYRAG	1 cut(s)	669
SmoI	CTYRAG	1 cut(s)	669
SphI	GCATGC	1 cut(s)	49
SspI	AATATT	2 cut(s)	1429, 2134
SspMI	CTAG	4 cut(s)	515, 1080, 2009, 2330
StyD4I	CCNGG	5 cut(s)	476, 1303, 1669, 2080, 2081
StyI	CCWWGG	5 cut(s)	63, 353, 849, 897, 991
TaaI	ACNGT	3 cut(s)	871, 930, 1630
TaiI	ACGT	5 cut(s)	158, 798, 1828, 2191, 2239
TaqI	TCGA	6 cut(s)	321, 465, 686, 782, 884, 1710
TatI	WGTACW	4 cut(s)	222, 360, 1635, 2161
TauI	GCSGC	4 cut(s)	45, 91, 121, 751
TfiI	GAWTC	4 cut(s)	812, 1073, 2321, 2360
Tru1I	TTAA	5 cut(s)	1170, 1496, 1625, 2091, 2271
Tru9I	TTAA	5 cut(s)	1170, 1496, 1625, 2091, 2271
TscAI	CASTG	5 cut(s)	933, 959, 1120, 1489, 1732
TseFI	GTSAC	2 cut(s)	865, 1115
TseI	GCWGC	7 cut(s)	718, 917, 1019, 1913, 1985, 2054, 2207
Tsp45I	GTSAC	2 cut(s)	865, 1115
TspMI	CCCGGG	1 cut(s)	2081
TspRI	CASTG	5 cut(s)	933, 959, 1120, 1489, 1732
Tth111I	GACNNNGTC	1 cut(s)	197
VpaK11BI	GGWCC	2 cut(s)	350, 1235
VspI	ATTAAT	1 cut(s)	2091
XapI	RAATTY	6 cut(s)	344, 643, 688, 1296, 1568, 1840
XbaI	TCTAGA	2 cut(s)	514, 2008
XceI	RCATGY	2 cut(s)	49, 223
XcmI	CCANNNNNNNNNTGG	1 cut(s)	211
XmaI	CCCGGG	1 cut(s)	2081
XmnI	GAANNNNTTC	1 cut(s)	1465
XspI	CTAG	4 cut(s)	515, 1080, 2009, 2330
Zsp2I	ATGCAT	1 cut(s)	544

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.