Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0004382

Physical Location & Seq

Genomic Coordinates Forward (+)

62346527 .. 62350555

Gene Structure 4029 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030483

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGCTCCACCTGGTAAAACCCCTCCGCCCCCATCTCCGCCATCTACGGTTGGCCCACTTCAAGGCCCCAAAGCCCCCAAAGCCGCCGGAATCCCCTCCGAAGCTGCCAAACCCTCCCCAAAAGCCCGAAACCTTCACCCTCCACGACCACACGTGGCAGGACCCCTACAGCTGGATGTCCAAGCTCAACGACAAGGTCGCCATGCGACACATGGACATGTACATGGAACAGGAAGACAAGTACACCGAAGCTGTCATGTACGGCACCGACCGCCTCCAGAGCAAGCTCCAGTCCGAGATGGCCTCCCGCTTATCCTTCGACCTCTCCACTCCTCCCCTCAAATTCGGACCATGGTTGTACTACCGGCGCGTGGAGGAAGGTAAGCAGTTTCCGGTGCTGTGTCGTCGACTCACGAGTTTACACGACGAGTTCATTTCTCACAAGTCGCCTTCTGCTGGGTTTGATTTTACTTCCGGGAAGAGAATTGAGCAGAAGCTGATTGATTACAATCAAGAAGCTCAAAGATTTGGAGGTTATGCATATGAAGAATTATCGGAAGTTTCACCGGATCATCGGTTTATTGCTTATACAATGTATGATAAAGACAAGGACTTGTTTAGGTTGTCTGTTAGGAACTTGAATTCTGGTGCATTGTGTAGTAAGCCTCAAGCGGAGCGTGTTTCGAATTTGGCTTGGGCCAAAGACGGGCAGGCATTGCTTTATGTTGTTACCGATCAGAACAAGCGGCCATATAGGATGTATTGTAGCATGATTGGATCGACTGATGAGGACGTTATGGTTTTTGAAGAATCGGATAATAATTTGTTTGTTAACATTAGACACACGAAAGATTTCCGGTTTGTGACTGTAAATACCTTCTCTACCGCGTCTTCCAAGGTCTTTTTGATAAATGCAGCTGATCCATTGTCAGGGTTGAACTTAGTTTGGCAGTGTGAAGGTATAGCGCATTGCGTACTTGAGCATCACCAAGGATATCTTTTTCTGTTTACAGATGCTGCCAAAGGAGGTAAGTCAGTTGATAACCATTATCTCCTTCGTAGTCCTGTGGATTCTTCGAGCAGTCCAAGAATATGGGAGGATGTATTCGTCAGTGACCCAGACTGGGTTTTTGAGGATGTGGATTTCAGTCATACACACTTGGTACTTAATATAAGGGAAGGGCGAAACTTTAGACTTTGTTCTGTTTCTTTGCCTTTGCCTGCTGGGAAGGGACCAGTTCATCTAAAAGAGCTTCACCCACAATTCTTGCCTCTTCCAAAATATGTTTCGCAAATTTCACCTGGACCAAATTATGACTACCATTCATCAAAGATGCGCTTTACAATATCGTCACCTGTGATGCCAGATGCTGTGGTTGATTATGACCTGTCAAATGGAAACTGGAAGATCATTCAACAGCAGAATATTCTTCATGAAAGAACAAAGGTTTTGTATGGAACAGCTTCTTCAGCAAGCATCAGCGAGAAAACCTTAAATACAGATAATTTGAATTCTGCAAATGAAGTCAGCCCTGATGATCAGCAGTTGTGGAATGATCTTTCTGAGTTTTATGCTTGTGAACAGCATAATGTCTCTTCTTATGATGGAGTCTTGGTTCCATTGACTGTTGTATACTCTTGCAAAAACAAGAAAGAGGATCAGAACCCTGGATTGCTTCATGTGCATGGAGCTTATGGTGAGCTACTCGATAAAAGGTGGCGCAGTGAGTTGAAAAGCCTTCTTGATTGTGGTTGGATGGTTGCCTATGGTCTGATTTGTGTTAAATACTACCAAGTTATAGGTGGAGGCGGTGGAGGTAAGAAGTGGCATCATGATGGAAGATGTACAAAGAAGATTAATTCCATCAAGGATTTTATAGCCTGTGCCAATTTCCTTGTTGACAAAGAAATTGTCCATGAAAAAAAGCTCGCTGCTTCAGGTTATAGTGCTGGAGGACTTGTAGTTGCTTCAGCCATCAATCAATGCCCACATTTGTTTACTGCTGCCGTTTTGAAGGTTCCATTTTTAGATGCAACGAGCACTCTTCTCTATCCCATTATACCGCTCACAGCTGTTGACTATGAAGAGTTTGGATACCCTGGGGACATTAATGATTTTCATGCTATACGTGACTATTCTCCATACGACAATATTCAGAAGGATGTTCTCTATCCAGCTGTATTGATATCCTCGTCTTTCAATACACGTTTTGGAGTGTGGGAAGCTGCAAAATGGGTTGCACGAGTTCGTGAACTTACAGTTTACGACCCAAAGCGTCCAATACTGCTTAATTTGACAACAGACATAGTAGAGGAAAACAGGTACCTCCAGTGCAAGGAATCGGCACTAGAGACTGCATTTCTTATAAAGGCAGTGGAATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0070011 GO:0070012 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S9 Peptidase_S9_N

Protein Analysis

795

Amino Acids

90.39

Weight (kDa)

6.36

Isoelectric Point (pI)

47.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S9_N	PF02897	39 - 474	4.2e-42	Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9	PF00326	590 - 751	1.3e-28	Prolyl oligopeptidase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66960
fragaria_vesca	FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740
malus_domestica	MD15G1274800.v1.1
prunus_persica	Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1
pyrus_communis	pycom15g23790
rosa_chinensis	RchiOBHm_Chr1g0319111 RchiOBHm_Chr2g0103681
rosa_laevigata	RLG00000017248 RLG00000029580 RLG00000030483
rosa_multiflora	Rmu_sc0001852.1_g000011 Rmu_sc0003574.1_g000010 Rmu_sc0009469.1_g000007
rosa_roxburghii	Rroxscaffold_2G00139490 Rroxscaffold_4G00328470
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh1AG038700 Rh2AG165200 Rh2BG172400 Rh2CG171300 Rh2DG170800 Rh5CG361300
rosa_wichuraiana	Rw1G003040 Rw2G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2366
Acc65I	GGTACC	1 cut(s)	2322
AccB1I	GGYRCC	2 cut(s)	263, 2322
AccBSI	CCGCTC	1 cut(s)	2065
AccI	GTMKAC	2 cut(s)	406, 1632
AccII	CGCG	2 cut(s)	369, 887
AclWI	GGATC	4 cut(s)	576, 784, 914, 1665
AcoI	YGGCCR	1 cut(s)	746
AcsI	RAATTY	5 cut(s)	341, 640, 685, 1293, 1510
AcuI	CTGAAG	3 cut(s)	1452, 1920, 1953
AcvI	CACGTG	1 cut(s)	153
AfaI	GTAC	8 cut(s)	221, 242, 260, 359, 975, 1164, 1846, 2324
AfiI	CCNNNNNNNGG	8 cut(s)	61, 171, 370, 455, 705, 929, 1123, 2335
AflIII	ACRYGT	3 cut(s)	150, 216, 2204
AgsI	TTSAA	9 cut(s)	61, 640, 806, 937, 1415, 1510, 1732, 2014, 2200
AjnI	CCWGG	4 cut(s)	9, 1300, 1666, 2098
AjuI	GAANNNNNNNTTGG	2 cut(s)	1270, 1302
AloI	GAACNNNNNNTCC	2 cut(s)	1599, 1631
Alw21I	GWGCWC	1 cut(s)	2042
Alw26I	GTCTC	2 cut(s)	1597, 2345
AlwI	GGATC	4 cut(s)	576, 784, 914, 1665
AlwNI	CAGNNNCTG	3 cut(s)	496, 1016, 1370
AoxI	GGCC	5 cut(s)	51, 63, 300, 696, 746
ApeKI	GCWGC	6 cut(s)	103, 914, 1016, 1931, 2003, 2225
ApoI	RAATTY	5 cut(s)	341, 640, 685, 1293, 1510
ArsI	GACNNNNNNTTYG	4 cut(s)	1112, 1144, 1896, 1928
AseI	ATTAAT	2 cut(s)	1857, 2109
Asp700I	GAANNNNTTC	2 cut(s)	851, 1462
Asp718I	GGTACC	1 cut(s)	2322
AspLEI	GCGC	4 cut(s)	369, 967, 1338, 1722
AspS9I	GGNCC	7 cut(s)	52, 64, 160, 347, 696, 1232, 1304
AsuC2I	CCSGG	1 cut(s)	475
AsuHPI	GGTGA	7 cut(s)	127, 555, 977, 1247, 1290, 1344, 1709
AsuII	TTCGAA	1 cut(s)	683
AvaII	GGWCC	4 cut(s)	160, 347, 1232, 1304
BanI	GGYRCC	2 cut(s)	263, 2322
BauI	CACGAG	2 cut(s)	412, 2241
BbrPI	CACGTG	1 cut(s)	153
BbsI	GAAGAC	2 cut(s)	240, 882
Bbv12I	GWGCWC	1 cut(s)	2042
BbvI	GCAGC	6 cut(s)	90, 926, 1003, 1918, 1990, 2212
BccI	CCATC	8 cut(s)	39, 48, 292, 1598, 1750, 1829, 1871, 1982
BceAI	ACGGC	2 cut(s)	277, 1991
BcgI	CGANNNNNNTGC	1 cut(s)	2361
BciT130I	CCWGG	4 cut(s)	11, 1302, 1668, 2100
BciVI	GTATCC	1 cut(s)	2087
BclI	TGATCA	1 cut(s)	1537
BcnI	CCSGG	1 cut(s)	475
BcoDI	GTCTC	2 cut(s)	1597, 2345
BfaI	CTAG	1 cut(s)	2348
BfmI	CTRYAG	1 cut(s)	166
BfuI	GTATCC	1 cut(s)	2087
BisI	GCNGC	8 cut(s)	83, 104, 746, 915, 1017, 1932, 2004, 2226
BlsI	GCNGC	8 cut(s)	84, 105, 747, 916, 1018, 1933, 2005, 2227
Bme1390I	CCNGG	5 cut(s)	11, 475, 1302, 1668, 2100
Bme18I	GGWCC	4 cut(s)	160, 347, 1232, 1304
BmgT120I	GGNCC	7 cut(s)	52, 64, 160, 347, 696, 1232, 1304
BmiI	GGNNCC	7 cut(s)	66, 162, 265, 1233, 1617, 2019, 2324
BmrFI	CCNGG	5 cut(s)	11, 475, 1302, 1668, 2100
BmrI	ACTGGG	1 cut(s)	1132
BmsI	GCATC	8 cut(s)	991, 1003, 1323, 1350, 1357, 1485, 1837, 2020
BmuI	ACTGGG	1 cut(s)	1132
BpiI	GAAGAC	2 cut(s)	240, 882
BplI	GAGNNNNNCTC	2 cut(s)	287, 319
BpmI	CTGGAG	4 cut(s)	260, 272, 1971, 2312
Bpu14I	TTCGAA	1 cut(s)	683
BpuEI	CTTGAG	2 cut(s)	651, 998
BpuMI	CCSGG	1 cut(s)	475
BsaAI	YACGTR	2 cut(s)	153, 2129
BsaBI	GATNNNNATC	1 cut(s)	507
BsaJI	CCNNGG	6 cut(s)	350, 894, 988, 1666, 2098, 2099
BsaWI	WCCGGW	3 cut(s)	391, 565, 855
BsaXI	ACNNNNNCTCC	4 cut(s)	1599, 1629, 1944, 1974
Bsc4I	CCNNNNNNNGG	8 cut(s)	61, 171, 370, 455, 705, 929, 1123, 2335
Bse118I	RCCGGY	1 cut(s)	363
Bse1I	ACTGG	5 cut(s)	289, 1127, 1235, 1406, 2329
Bse3DI	GCAATG	2 cut(s)	713, 967
Bse8I	GATNNNNATC	1 cut(s)	507
BseBI	CCWGG	4 cut(s)	11, 1302, 1668, 2100
BseDI	CCNNGG	6 cut(s)	350, 894, 988, 1666, 2098, 2099
BseGI	GGATG	6 cut(s)	180, 762, 1105, 1141, 1761, 2167
BseJI	GATNNNNATC	1 cut(s)	507
BseLI	CCNNNNNNNGG	8 cut(s)	61, 171, 370, 455, 705, 929, 1123, 2335
BseMI	GCAATG	2 cut(s)	713, 967
BseMII	CTCAG	1 cut(s)	1554
BseNI	ACTGG	5 cut(s)	289, 1127, 1235, 1406, 2329
BseRI	GAGGAG	1 cut(s)	321
BseXI	GCAGC	6 cut(s)	90, 926, 1003, 1918, 1990, 2212
BseYI	CCCAGC	2 cut(s)	455, 1223
Bsh1236I	CGCG	2 cut(s)	369, 887
Bsh1285I	CGRYCG	1 cut(s)	271
BshFI	GGCC	5 cut(s)	53, 65, 302, 698, 748
BshNI	GGYRCC	2 cut(s)	263, 2322
BsiEI	CGRYCG	1 cut(s)	271
BsiHKAI	GWGCWC	1 cut(s)	2042
BsiSI	CCGG	6 cut(s)	86, 364, 392, 474, 566, 856
BslFI	GGGAC	2 cut(s)	1245, 2117
BslI	CCNNNNNNNGG	8 cut(s)	61, 171, 370, 455, 705, 929, 1123, 2335
BsmAI	GTCTC	2 cut(s)	1597, 2345
BsmFI	GGGAC	2 cut(s)	1245, 2117
BsnI	GGCC	5 cut(s)	53, 65, 302, 698, 748
Bsp119I	TTCGAA	1 cut(s)	683
Bsp1286I	GDGCHC	1 cut(s)	2042
Bsp1407I	TGTACA	2 cut(s)	219, 1844
Bsp143I	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
Bsp19I	CCATGG	1 cut(s)	350
BspANI	GGCC	5 cut(s)	53, 65, 302, 698, 748
BspCNI	CTCAG	1 cut(s)	1555
BspFNI	CGCG	2 cut(s)	369, 887
BspHI	TCATGA	2 cut(s)	1432, 1831
BspLI	GGNNCC	7 cut(s)	66, 162, 265, 1233, 1617, 2019, 2324
BspPI	GGATC	4 cut(s)	576, 784, 914, 1665
BspT104I	TTCGAA	1 cut(s)	683
BspT107I	GGYRCC	2 cut(s)	263, 2322
BsrBI	CCGCTC	1 cut(s)	2065
BsrDI	GCAATG	2 cut(s)	713, 967
BsrFI	RCCGGY	1 cut(s)	363
BsrGI	TGTACA	2 cut(s)	219, 1844
BsrI	ACTGG	5 cut(s)	289, 1127, 1235, 1406, 2329
BssAI	RCCGGY	1 cut(s)	363
BssECI	CCNNGG	6 cut(s)	350, 894, 988, 1666, 2098, 2099
BssMI	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
BssNAI	GTATAC	1 cut(s)	1633
BssSI	CACGAG	2 cut(s)	412, 2241
BssT1I	CCWWGG	3 cut(s)	350, 894, 988
Bst1107I	GTATAC	1 cut(s)	1633
Bst2BI	CACGAG	2 cut(s)	412, 2241
Bst2UI	CCWGG	4 cut(s)	11, 1302, 1668, 2100
Bst4CI	ACNGT	4 cut(s)	48, 868, 1627, 2260
Bst6I	CTCTTC	5 cut(s)	473, 1278, 1600, 2049, 2079
BstAPI	GCANNNNNTGC	1 cut(s)	715
BstAUI	TGTACA	2 cut(s)	219, 1844
BstBAI	YACGTR	2 cut(s)	153, 2129
BstBI	TTCGAA	1 cut(s)	683
BstC8I	GCNNGC	5 cut(s)	284, 711, 1221, 1474, 1929
BstDEI	CTNAG	2 cut(s)	940, 1563
BstDSI	CCRYGG	1 cut(s)	350
BstF5I	GGATG	6 cut(s)	180, 762, 1105, 1141, 1761, 2167
BstFNI	CGCG	2 cut(s)	369, 887
BstHHI	GCGC	4 cut(s)	369, 967, 1338, 1722
BstKTI	GATC	8 cut(s)	571, 736, 779, 922, 1410, 1540, 1558, 1660
BstMAI	GTCTC	2 cut(s)	1597, 2345
BstMBI	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
BstMCI	CGRYCG	1 cut(s)	271
BstMWI	GCNNNNNNNGC	6 cut(s)	79, 270, 279, 715, 1469, 1681
BstNI	CCWGG	4 cut(s)	11, 1302, 1668, 2100
BstNSI	RCATGY	1 cut(s)	220
BstSCI	CCNGG	5 cut(s)	9, 473, 1300, 1666, 2098
BstSFI	CTRYAG	1 cut(s)	166
BstUI	CGCG	2 cut(s)	369, 887
BstV1I	GCAGC	6 cut(s)	90, 926, 1003, 1918, 1990, 2212
BstV2I	GAAGAC	2 cut(s)	240, 882
BstXI	CCANNNNNNTGG	1 cut(s)	1092
BstZ17I	GTATAC	1 cut(s)	1633
BsuI	GTATCC	1 cut(s)	2087
BsuRI	GGCC	5 cut(s)	53, 65, 302, 698, 748
BtgI	CCRYGG	1 cut(s)	350
BtsCI	GGATG	6 cut(s)	180, 762, 1105, 1141, 1761, 2167
BtsI	GCAGTG	3 cut(s)	956, 1729, 2379
BtsIMutI	CAGTG	5 cut(s)	956, 1117, 1729, 2336, 2379
Cac8I	GCNNGC	5 cut(s)	284, 711, 1221, 1474, 1929
CaiI	CAGNNNCTG	3 cut(s)	496, 1016, 1370
CciI	TCATGA	2 cut(s)	1432, 1831
CfoI	GCGC	4 cut(s)	369, 967, 1338, 1722
Cfr10I	RCCGGY	1 cut(s)	363
Cfr13I	GGNCC	7 cut(s)	52, 64, 160, 347, 696, 1232, 1304
CseI	GACGC	2 cut(s)	876, 2264
CsiI	ACCWGGT	1 cut(s)	9
Csp6I	GTAC	8 cut(s)	220, 241, 259, 358, 974, 1163, 1845, 2323
CspCI	CAANNNNNGTGG	2 cut(s)	1248, 1283
CviQI	GTAC	8 cut(s)	220, 241, 259, 358, 974, 1163, 1845, 2323
DdeI	CTNAG	2 cut(s)	940, 1563
DpnI	GATC	8 cut(s)	570, 735, 778, 921, 1409, 1539, 1557, 1659
DpnII	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
EaeI	YGGCCR	1 cut(s)	746
Eam1104I	CTCTTC	5 cut(s)	473, 1278, 1600, 2049, 2079
EarI	CTCTTC	5 cut(s)	473, 1278, 1600, 2049, 2079
EciI	GGCGGA	2 cut(s)	14, 26
Eco130I	CCWWGG	3 cut(s)	350, 894, 988
Eco32I	GATATC	2 cut(s)	995, 2187
Eco47I	GGWCC	4 cut(s)	160, 347, 1232, 1304
Eco57I	CTGAAG	3 cut(s)	1452, 1920, 1953
Eco72I	CACGTG	1 cut(s)	153
EcoO109I	RGGNCCY	2 cut(s)	64, 160
EcoRI	GAATTC	2 cut(s)	640, 1510
EcoRII	CCWGG	4 cut(s)	9, 1300, 1666, 2098
EcoRV	GATATC	2 cut(s)	995, 2187
EcoT14I	CCWWGG	3 cut(s)	350, 894, 988
EcoT22I	ATGCAT	1 cut(s)	541
ErhI	CCWWGG	3 cut(s)	350, 894, 988
FalI	AAGNNNNNCTT	4 cut(s)	981, 1013, 1322, 1354
FaqI	GGGAC	2 cut(s)	1245, 2117
FauI	CCCGC	1 cut(s)	314
FauNDI	CATATG	1 cut(s)	541
FbaI	TGATCA	1 cut(s)	1537
FblI	GTMKAC	2 cut(s)	406, 1632
Fnu4HI	GCNGC	8 cut(s)	83, 104, 746, 915, 1017, 1932, 2004, 2226
FokI	GGATG	6 cut(s)	187, 769, 1112, 1148, 1768, 2174
Fsp4HI	GCNGC	8 cut(s)	83, 104, 746, 915, 1017, 1932, 2004, 2226
FspBI	CTAG	1 cut(s)	2348
GlaI	GCGC	4 cut(s)	368, 966, 1337, 1721
GluI	GCNGC	8 cut(s)	83, 104, 746, 915, 1017, 1932, 2004, 2226
GsaI	CCCAGC	2 cut(s)	459, 1227
GsuI	CTGGAG	4 cut(s)	260, 272, 1971, 2312
HaeIII	GGCC	5 cut(s)	53, 65, 302, 698, 748
HapII	CCGG	6 cut(s)	86, 364, 392, 474, 566, 856
HgaI	GACGC	2 cut(s)	876, 2264
HhaI	GCGC	4 cut(s)	369, 967, 1338, 1722
Hin6I	GCGC	4 cut(s)	367, 965, 1336, 1720
HinP1I	GCGC	4 cut(s)	367, 965, 1336, 1720
HincII	GTYRAC	4 cut(s)	407, 832, 1900, 2077
HindII	GTYRAC	4 cut(s)	407, 832, 1900, 2077
HinfI	GANTC	7 cut(s)	89, 408, 809, 1070, 1608, 2339, 2378
HpaI	GTTAAC	1 cut(s)	832
HpaII	CCGG	6 cut(s)	86, 364, 392, 474, 566, 856
HphI	GGTGA	7 cut(s)	127, 555, 977, 1247, 1290, 1344, 1709
Hpy188III	TCNNGA	7 cut(s)	277, 412, 512, 1433, 1742, 1832, 2249
Hpy99I	CGWCG	2 cut(s)	408, 428
HpyCH4III	ACNGT	4 cut(s)	48, 868, 1627, 2260
HpyCH4IV	ACGT	4 cut(s)	152, 792, 2128, 2206
HpyF10VI	GCNNNNNNNGC	6 cut(s)	79, 270, 279, 715, 1469, 1681
HpyF3I	CTNAG	2 cut(s)	940, 1563
HpySE526I	ACGT	4 cut(s)	152, 792, 2128, 2206
HspAI	GCGC	4 cut(s)	367, 965, 1336, 1720
KpnI	GGTACC	1 cut(s)	2326
Ksp22I	TGATCA	1 cut(s)	1537
KspAI	GTTAAC	1 cut(s)	832
Kzo9I	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
LmnI	GCTCC	4 cut(s)	9, 291, 673, 1688
Lsp1109I	GCAGC	6 cut(s)	90, 926, 1003, 1918, 1990, 2212
LweI	GCATC	8 cut(s)	991, 1003, 1323, 1350, 1357, 1485, 1837, 2020
MabI	ACCWGGT	1 cut(s)	9
MaeI	CTAG	1 cut(s)	2348
MaeII	ACGT	4 cut(s)	152, 792, 2128, 2206
MaeIII	GTNAC	5 cut(s)	727, 862, 1112, 1350, 2129
MalI	GATC	8 cut(s)	570, 735, 778, 921, 1409, 1539, 1557, 1659
MbiI	CCGCTC	1 cut(s)	2065
MboI	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
MhlI	GDGCHC	1 cut(s)	2042
MlyI	GAGTC	2 cut(s)	402, 1617
MmeI	TCCRAC	1 cut(s)	1733
Mph1103I	ATGCAT	1 cut(s)	541
MroXI	GAANNNNTTC	2 cut(s)	851, 1462
MseI	TTAA	7 cut(s)	831, 1167, 1493, 1782, 1857, 2109, 2289
MslI	CAYNNNNRTG	4 cut(s)	215, 221, 1683, 1833
MspA1I	CMGCKG	4 cut(s)	171, 917, 2072, 2177
MspI	CCGG	6 cut(s)	86, 364, 392, 474, 566, 856
MspR9I	CCNGG	5 cut(s)	11, 475, 1302, 1668, 2100
MvaI	CCWGG	4 cut(s)	11, 1302, 1668, 2100
MvnI	CGCG	2 cut(s)	369, 887
MwoI	GCNNNNNNNGC	6 cut(s)	79, 270, 279, 715, 1469, 1681
NciI	CCSGG	1 cut(s)	475
NcoI	CCATGG	1 cut(s)	350
NdeI	CATATG	1 cut(s)	541
NdeII	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
NlaIV	GGNNCC	7 cut(s)	66, 162, 265, 1233, 1617, 2019, 2324
NmuCI	GTSAC	4 cut(s)	862, 1112, 1350, 2129
NsiI	ATGCAT	1 cut(s)	541
NspI	RCATGY	1 cut(s)	220
NspV	TTCGAA	1 cut(s)	683
PagI	TCATGA	2 cut(s)	1432, 1831
PasI	CCCWGGG	1 cut(s)	2099
PciI	ACATGT	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	851, 1462
PfeI	GAWTC	5 cut(s)	89, 809, 1070, 2339, 2378
PflFI	GACNNNGTC	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	473
PkrI	GCNGC	8 cut(s)	84, 105, 747, 916, 1018, 1933, 2005, 2227
PleI	GAGTC	2 cut(s)	402, 1616
PmaCI	CACGTG	1 cut(s)	153
PmlI	CACGTG	1 cut(s)	153
PpsI	GAGTC	2 cut(s)	402, 1616
Ppu21I	YACGTR	2 cut(s)	153, 2129
PpuMI	RGGWCCY	1 cut(s)	160
PscI	ACATGT	1 cut(s)	216
PshBI	ATTAAT	2 cut(s)	1857, 2109
PsiI	TTATAA	1 cut(s)	2366
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	4 cut(s)	9, 1300, 1666, 2098
PspCI	CACGTG	1 cut(s)	153
PspFI	CCCAGC	2 cut(s)	455, 1223
PspGI	CCWGG	4 cut(s)	9, 1300, 1666, 2098
PspN4I	GGNNCC	7 cut(s)	66, 162, 265, 1233, 1617, 2019, 2324
PspPI	GGNCC	7 cut(s)	52, 64, 160, 347, 696, 1232, 1304
PspPPI	RGGWCCY	1 cut(s)	160
PstNI	CAGNNNCTG	3 cut(s)	496, 1016, 1370
PsyI	GACNNNGTC	1 cut(s)	194
PvuII	CAGCTG	4 cut(s)	171, 917, 2072, 2177
RsaI	GTAC	8 cut(s)	221, 242, 260, 359, 975, 1164, 1846, 2324
RsaNI	GTAC	8 cut(s)	220, 241, 259, 358, 974, 1163, 1845, 2323
RseI	CAYNNNNRTG	4 cut(s)	215, 221, 1683, 1833
SalI	GTCGAC	1 cut(s)	405
SaqAI	TTAA	7 cut(s)	831, 1167, 1493, 1782, 1857, 2109, 2289
SatI	GCNGC	8 cut(s)	83, 104, 746, 915, 1017, 1932, 2004, 2226
Sau3AI	GATC	8 cut(s)	568, 733, 776, 919, 1407, 1537, 1555, 1657
Sau96I	GGNCC	7 cut(s)	52, 64, 160, 347, 696, 1232, 1304
SchI	GAGTC	2 cut(s)	402, 1617
ScrFI	CCNGG	5 cut(s)	11, 475, 1302, 1668, 2100
SduI	GDGCHC	1 cut(s)	2042
SexAI	ACCWGGT	1 cut(s)	9
SfaNI	GCATC	8 cut(s)	991, 1003, 1323, 1350, 1357, 1485, 1837, 2020
SfcI	CTRYAG	1 cut(s)	166
SfuI	TTCGAA	1 cut(s)	683
SinI	GGWCC	4 cut(s)	160, 347, 1232, 1304
SmiMI	CAYNNNNRTG	4 cut(s)	215, 221, 1683, 1833
SmlI	CTYRAG	2 cut(s)	666, 977
SmoI	CTYRAG	2 cut(s)	666, 977
SspI	AATATT	2 cut(s)	1426, 2152
SspMI	CTAG	1 cut(s)	2348
StyD4I	CCNGG	5 cut(s)	9, 473, 1300, 1666, 2098
StyI	CCWWGG	3 cut(s)	350, 894, 988
TaaI	ACNGT	4 cut(s)	48, 868, 1627, 2260
TaiI	ACGT	4 cut(s)	155, 795, 2131, 2209
TaqI	TCGA	6 cut(s)	318, 406, 683, 779, 1076, 1707
TatI	WGTACW	4 cut(s)	219, 240, 357, 1844
TauI	GCSGC	2 cut(s)	85, 748
TfiI	GAWTC	5 cut(s)	89, 809, 1070, 2339, 2378
Tru1I	TTAA	7 cut(s)	831, 1167, 1493, 1782, 1857, 2109, 2289
Tru9I	TTAA	7 cut(s)	831, 1167, 1493, 1782, 1857, 2109, 2289
TscAI	CASTG	5 cut(s)	956, 1117, 1729, 2336, 2379
TseFI	GTSAC	4 cut(s)	862, 1112, 1350, 2129
TseI	GCWGC	6 cut(s)	103, 914, 1016, 1931, 2003, 2225
Tsp45I	GTSAC	4 cut(s)	862, 1112, 1350, 2129
TspRI	CASTG	5 cut(s)	956, 1117, 1729, 2336, 2379
Tth111I	GACNNNGTC	1 cut(s)	194
VpaK11BI	GGWCC	4 cut(s)	160, 347, 1232, 1304
VspI	ATTAAT	2 cut(s)	1857, 2109
XapI	RAATTY	5 cut(s)	341, 640, 685, 1293, 1510
XceI	RCATGY	1 cut(s)	220
XcmI	CCANNNNNNNNNTGG	2 cut(s)	208, 1799
XmiI	GTMKAC	2 cut(s)	406, 1632
XmnI	GAANNNNTTC	2 cut(s)	851, 1462
XspI	CTAG	1 cut(s)	2348
Zsp2I	ATGCAT	1 cut(s)	541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.