Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004382

Physical Location & Seq

Genomic Coordinates Forward (+)

24129826 .. 24133993

Gene Structure 4168 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1274800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGCGCCACCTCCTAAAACCCCTCCGCCACCATTTCCGCCGTCTGCGGTGGGCCCACCACAAGGCCCAGAAGCCGGCACCGACCCCCTCAGCGCAACCGAGACCGCCACGGCCGCCGCAGAAGCCCGAGAGCTTCACCTTCCACGACCACACGTGGGAGGACCCGTACAACTGGATGTCGAAGCTCAACGACAAGGTGGCGATGCGGCACCTGGATATGTACATGGAGCAAGAGGAGAAGTACACGGAGGCCGTCATGTCCTGCACCGACCGCCTCCAGAGCAAGCTCCAGTCCGAGATGGCCTCTCGCATCGCCTCCGACCTCGCCACCCCTCCCCTTCGCTGGGGCCCCTGGTTGTACTACCGGCGCGTGGAGGAGGGGAAACAATTTCCAGTGCTCTGCCGGAGACTAGCGAGTTTAAACGACGAGTTCATTTCACACAAATCGCCGTCTTCCGGATTCGATTTCACTTCGGGAAAAAGGATCGAGCAGAAGCTCATTGATTACAATCAGGAATCACAAAGATATGGCGGTTATGCTTACGAAGAGTTATCGGAAGTATCGCCGGATCATCGGTTTATAGCTTACACAATGTATGATAAGGAGAGTGACTTGTTTAGATTATCTGTTAGGAATTTGAATTCTGGTGCATTGTGTAGTAAGCCGAAAGCAGAGCGTGTTTCGAATTTGGCTTGGGCCAAAGACGGGCAGGCATTGCTTTATGTTGTCACTGATGAAAACAAGCGGCCTTATAGGATATATTGTAGCACGATTGGATCAACCGATGATGATGTTTTGATTCTGGAAGAACCAGATGAAAATGTTTATGTTAACATCCGACATACTAAAAATTTCCAGTTTGTGACAGTGCATACTTTTTCCACGACGTCTTCCAAGGTGTTTTTGATAAATGCAGCTGATCCATTGTCCGGGATGAACTTGGTTTGGGAGTGTGAAGGTATAGCCCATGGCATACTTGAGCATCATCAAGGATATCTTTATCTGTTTACTGATGCTTCAAAAGAGGGCCAGTCAACCGATTACCATTTTCTTCTTCGTAGTCCTCTAGATTCTTCTTCAAGTCCAAGAATATGGGAGAACGTATTCGCCAATGACAAGGACTGGATTTTTGAGGATTTTGATTTCAGTCATACACACTTAGTAGTTATTTTAAAGGAAGGGCGAAACTTTAAACTTTGTTCAGTCACTTTACCTTTGCCTGCTGGTAAGGGGGCAGTGCATCTAAAAGAGCTTCACCCACAATTCCTGCCCCTTCCAAAATATGTTTCGCAAATATCACCTGGACCAAATTATGATTACCATTCATCAGTGATGCGCTTCACAATATCATCACCTGTGATGCCGGATGCTGTGGTTGATTATGACCTGTCAAATGGAAAGTGGAATATCATTCAACAGCAGAATATTCTTCATGAAAGAACACGGGTTTTGTATGGAACAACTTCCATAGAAAGCATCAACGAGGAGACCATAAATTCAAAACCTTTGAATTTTATAACTGATGCCAACTCTGATGATGCTCAGTTGTGGAATGATCTTTCTGAGTTTTATGCTTGTGAACAATATAATGTATCTTCTTATGATGGAGTTTTGGTTCCATTGACTGTTGTATACTCCCGTAAAAACAAGAAAGAGGATCAGAACCCTGGATTGCTTCATGTCCATGGAGCTTATGGTGAGCTTCTGGACAAGCGGTGGCGGAGTGAGTTGAAAAGCCTTCTTGATCGTGGTTGGACCATTGCGTATGCTGACATTAGAGGCGGAGGAGGTGGAGGAAAGAAGTGGCACCATGATGGAAGGTGTACAAAGAAGATGAATTCCATCAAAGATTACATCTCCTGTGCCAAATTCCTTGTTGACAAAGAAATTGTCCATGAAAACAAGCTTGCTGGTTGGGGTCATAGTGCTGGAGGACTCGTGGTTGCTTCAGCCATCAATCAATCCCCATATTTATTTAAGGCTGCAATTTTGAAGGTTCCATTTTTAGACGCAACAAACACTCTTCTCTCTCCCATTTTACCACTTACAGCTGCCGACTATGAAGAGTTTGGATACCCTGGGGACATTGATGATTTTCATGCTATACGTGACTATTCTCCTTATGACAATATCCAGAAGGATGTTCTTTATCCAGCTGTCATGATCTCCTCATCTTTCAATACACGATTTGGAGTTTGGGAATCTGCAAAATGGATCGCGCGGGTTCGTGAACTTACAATTTATGACCAAAATCGTCCGGTACTGCTTAATTTAACAACAGATATAGTGGAGGAAAACAGGTACCTCCAGTGCAAGGAGTCGGCACTGGAGTCTGCATTTCTTATAAAGGTGATGGAATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0070011 GO:0070012 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S9 Peptidase_S9_N

Protein Analysis

788

Amino Acids

90.28

Weight (kDa)

6.2

Isoelectric Point (pI)

53.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S9_N	PF02897	40 - 473	2.5e-41	Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9	PF00326	575 - 749	3.3e-35	Prolyl oligopeptidase family
Hydrolase_4	PF12146	578 - 683	1.1e-06	Serine aminopeptidase, S33

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004382)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66960
fragaria_vesca	FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740 FvH4_1g14740
malus_domestica	MD15G1274800.v1.1
prunus_persica	Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1 Prupe.7G143000_v2.0.a1
pyrus_communis	pycom15g23790
rosa_chinensis	RchiOBHm_Chr1g0319111 RchiOBHm_Chr2g0103681
rosa_laevigata	RLG00000017248 RLG00000029580 RLG00000030483
rosa_multiflora	Rmu_sc0001852.1_g000011 Rmu_sc0003574.1_g000010 Rmu_sc0009469.1_g000007
rosa_roxburghii	Rroxscaffold_2G00139490 Rroxscaffold_4G00328470
rosa_rugosa	Rorug05G0248700
rosa_samantha	Rh1AG038700 Rh2AG165200 Rh2BG172400 Rh2CG171300 Rh2DG170800 Rh5CG361300
rosa_wichuraiana	Rw1G003040 Rw2G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1517, 2345
AatII	GACGTC	1 cut(s)	890
Acc65I	GGTACC	1 cut(s)	2301
AccB1I	GGYRCC	4 cut(s)	76, 207, 1806, 2301
AccI	GTMKAC	1 cut(s)	1632
AccII	CGCG	3 cut(s)	369, 2219, 2221
AccIII	TCCGGA	1 cut(s)	455
AclWI	GGATC	6 cut(s)	491, 576, 784, 914, 1665, 2222
AcoI	YGGCCR	1 cut(s)	110
AcsI	RAATTY	8 cut(s)	634, 640, 685, 850, 1495, 1510, 1837, 1868
AcuI	CTGAAG	1 cut(s)	1932
AcvI	CACGTG	1 cut(s)	153
AcyI	GRCGYC	1 cut(s)	887
AfaI	GTAC	7 cut(s)	167, 221, 242, 359, 1825, 2262, 2303
AflIII	ACRYGT	1 cut(s)	150
AgsI	TTSAA	9 cut(s)	640, 1020, 1080, 1415, 1500, 1510, 1732, 1993, 2179
AjnI	CCWGG	5 cut(s)	210, 350, 1300, 1666, 2077
AjuI	GAANNNNNNNTTGG	2 cut(s)	1270, 1302
AloI	GAACNNNNNNTCC	2 cut(s)	1599, 1631
Alw21I	GWGCWC	1 cut(s)	399
Alw26I	GTCTC	3 cut(s)	94, 400, 1481
AlwI	GGATC	6 cut(s)	491, 576, 784, 914, 1665, 2222
Ama87I	CYCGRG	1 cut(s)	125
Aor13HI	TCCGGA	1 cut(s)	455
AoxI	GGCC	9 cut(s)	51, 63, 110, 249, 300, 346, 696, 746, 1027
ApaI	GGGCCC	2 cut(s)	55, 350
ApeKI	GCWGC	3 cut(s)	914, 1982, 2051
ApoI	RAATTY	8 cut(s)	634, 640, 685, 850, 1495, 1510, 1837, 1868
ArsI	GACNNNNNNTTYG	4 cut(s)	1112, 1144, 1875, 1907
Asp700I	GAANNNNTTC	2 cut(s)	1103, 1462
Asp718I	GGTACC	1 cut(s)	2301
AspLEI	GCGC	5 cut(s)	6, 94, 369, 1338, 2221
AsuC2I	CCSGG	1 cut(s)	931
AsuHPI	GGTGA	6 cut(s)	127, 1247, 1290, 1344, 1709, 2362
AsuII	TTCGAA	1 cut(s)	683
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	3 cut(s)	160, 1304, 1755
BaeGI	GKGCMC	2 cut(s)	55, 350
BanI	GGYRCC	4 cut(s)	76, 207, 1806, 2301
BanII	GRGCYC	2 cut(s)	55, 350
BauI	CACGAG	1 cut(s)	1937
BbrPI	CACGTG	1 cut(s)	153
BbsI	GAAGAC	2 cut(s)	444, 882
Bbv12I	GWGCWC	1 cut(s)	399
BbvCI	CCTCAGC	1 cut(s)	88
BbvI	GCAGC	3 cut(s)	926, 1969, 2038
BccI	CCATC	6 cut(s)	292, 1598, 1808, 1850, 1961, 2347
BceAI	ACGGC	4 cut(s)	24, 125, 236, 433
BciT130I	CCWGG	5 cut(s)	212, 352, 1302, 1668, 2079
BciVI	GTATCC	1 cut(s)	2066
BcnI	CCSGG	1 cut(s)	931
BcoDI	GTCTC	3 cut(s)	94, 400, 1481
BfaI	CTAG	2 cut(s)	410, 1067
BfuI	GTATCC	1 cut(s)	2066
BisI	GCNGC	7 cut(s)	113, 116, 206, 746, 915, 1983, 2052
BlsI	GCNGC	7 cut(s)	114, 117, 207, 747, 916, 1984, 2053
Bme1390I	CCNGG	6 cut(s)	212, 352, 931, 1302, 1668, 2079
Bme18I	GGWCC	3 cut(s)	160, 1304, 1755
BmeT110I	CYCGRG	1 cut(s)	125
BmrFI	CCNGG	6 cut(s)	212, 352, 931, 1302, 1668, 2079
BpiI	GAAGAC	2 cut(s)	444, 882
BplI	GAGNNNNNCTC	2 cut(s)	287, 319
BpmI	CTGGAG	5 cut(s)	260, 272, 1950, 2291, 2348
Bpu10I	CCTNAGC	1 cut(s)	88
Bpu14I	TTCGAA	1 cut(s)	683
BpuEI	CTTGAG	1 cut(s)	998
BpuMI	CCSGG	1 cut(s)	931
BsaAI	YACGTR	2 cut(s)	153, 2108
BsaBI	GATNNNNATC	1 cut(s)	507
BsaHI	GRCGYC	1 cut(s)	887
BsaI	GGTCTC	2 cut(s)	94, 1481
BsaJI	CCNNGG	8 cut(s)	107, 350, 894, 967, 1666, 1684, 2077, 2078
BsaWI	WCCGGW	2 cut(s)	455, 2257
BsaXI	ACNNNNNCTCC	6 cut(s)	149, 179, 1599, 1629, 1923, 1953
Bse118I	RCCGGY	2 cut(s)	73, 363
Bse1I	ACTGG	8 cut(s)	176, 289, 392, 856, 1030, 1127, 2308, 2331
Bse3DI	GCAATG	2 cut(s)	713, 1758
Bse8I	GATNNNNATC	1 cut(s)	507
BseAI	TCCGGA	1 cut(s)	455
BseBI	CCWGG	5 cut(s)	212, 352, 1302, 1668, 2079
BseDI	CCNNGG	8 cut(s)	107, 350, 894, 967, 1666, 1684, 2077, 2078
BseGI	GGATG	5 cut(s)	180, 834, 939, 1372, 2146
BseJI	GATNNNNATC	1 cut(s)	507
BseMI	GCAATG	2 cut(s)	713, 1758
BseMII	CTCAG	3 cut(s)	102, 1554, 1556
BseNI	ACTGG	8 cut(s)	176, 289, 392, 856, 1030, 1127, 2308, 2331
BseRI	GAGGAG	5 cut(s)	248, 389, 1499, 1800, 2158
BseSI	GKGCMC	2 cut(s)	55, 350
BseX3I	CGGCCG	1 cut(s)	110
BseXI	GCAGC	3 cut(s)	926, 1969, 2038
BseYI	CCCAGC	1 cut(s)	342
BsgI	GTGCAG	1 cut(s)	247
Bsh1236I	CGCG	3 cut(s)	369, 2219, 2221
Bsh1285I	CGRYCG	2 cut(s)	113, 271
BshFI	GGCC	9 cut(s)	53, 65, 112, 251, 302, 348, 698, 748, 1029
BshNI	GGYRCC	4 cut(s)	76, 207, 1806, 2301
BsiEI	CGRYCG	2 cut(s)	113, 271
BsiHKAI	GWGCWC	1 cut(s)	399
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	8 cut(s)	74, 364, 403, 456, 566, 930, 1364, 2258
BslFI	GGGAC	1 cut(s)	2096
BsmAI	GTCTC	3 cut(s)	94, 400, 1481
BsmFI	GGGAC	1 cut(s)	2096
BsnI	GGCC	9 cut(s)	53, 65, 112, 251, 302, 348, 698, 748, 1029
Bso31I	GGTCTC	2 cut(s)	94, 1481
BsoBI	CYCGRG	1 cut(s)	125
Bsp119I	TTCGAA	1 cut(s)	683
Bsp120I	GGGCCC	2 cut(s)	51, 346
Bsp1286I	GDGCHC	3 cut(s)	55, 350, 399
Bsp13I	TCCGGA	1 cut(s)	455
Bsp1407I	TGTACA	2 cut(s)	219, 1823
Bsp143I	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
Bsp19I	CCATGG	2 cut(s)	967, 1684
BspANI	GGCC	9 cut(s)	53, 65, 112, 251, 302, 348, 698, 748, 1029
BspCNI	CTCAG	3 cut(s)	101, 1555, 1555
BspEI	TCCGGA	1 cut(s)	455
BspFNI	CGCG	3 cut(s)	369, 2219, 2221
BspHI	TCATGA	2 cut(s)	1432, 2160
BspPI	GGATC	6 cut(s)	491, 576, 784, 914, 1665, 2222
BspT104I	TTCGAA	1 cut(s)	683
BspT107I	GGYRCC	4 cut(s)	76, 207, 1806, 2301
BspTNI	GGTCTC	2 cut(s)	94, 1481
BsrDI	GCAATG	2 cut(s)	713, 1758
BsrFI	RCCGGY	2 cut(s)	73, 363
BsrGI	TGTACA	2 cut(s)	219, 1823
BsrI	ACTGG	8 cut(s)	176, 289, 392, 856, 1030, 1127, 2308, 2331
BssAI	RCCGGY	2 cut(s)	73, 363
BssECI	CCNNGG	8 cut(s)	107, 350, 894, 967, 1666, 1684, 2077, 2078
BssMI	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
BssNAI	GTATAC	1 cut(s)	1633
BssNI	GRCGYC	1 cut(s)	887
BssSI	CACGAG	1 cut(s)	1937
BssT1I	CCWWGG	3 cut(s)	894, 967, 1684
Bst1107I	GTATAC	1 cut(s)	1633
Bst2BI	CACGAG	1 cut(s)	1937
Bst2UI	CCWGG	5 cut(s)	212, 352, 1302, 1668, 2079
Bst4CI	ACNGT	2 cut(s)	868, 1627
Bst6I	CTCTTC	3 cut(s)	540, 2028, 2058
BstACI	GRCGYC	1 cut(s)	887
BstAPI	GCANNNNNTGC	1 cut(s)	715
BstAUI	TGTACA	2 cut(s)	219, 1823
BstBAI	YACGTR	2 cut(s)	153, 2108
BstBI	TTCGAA	1 cut(s)	683
BstC8I	GCNNGC	5 cut(s)	75, 284, 711, 1221, 1908
BstDEI	CTNAG	4 cut(s)	88, 1159, 1542, 1563
BstDSI	CCRYGG	3 cut(s)	107, 967, 1684
BstF5I	GGATG	5 cut(s)	180, 834, 939, 1372, 2146
BstFNI	CGCG	3 cut(s)	369, 2219, 2221
BstHHI	GCGC	5 cut(s)	6, 94, 369, 1338, 2221
BstKTI	GATC	9 cut(s)	486, 571, 779, 922, 1558, 1660, 1747, 2166, 2217
BstMAI	GTCTC	3 cut(s)	94, 400, 1481
BstMBI	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
BstMCI	CGRYCG	2 cut(s)	113, 271
BstMWI	GCNNNNNNNGC	5 cut(s)	112, 121, 270, 279, 715
BstNI	CCWGG	5 cut(s)	212, 352, 1302, 1668, 2079
BstSCI	CCNGG	6 cut(s)	210, 350, 929, 1300, 1666, 2077
BstSLI	GKGCMC	2 cut(s)	55, 350
BstUI	CGCG	3 cut(s)	369, 2219, 2221
BstV1I	GCAGC	3 cut(s)	926, 1969, 2038
BstV2I	GAAGAC	2 cut(s)	444, 882
BstXI	CCANNNNNNTGG	1 cut(s)	1092
BstZ17I	GTATAC	1 cut(s)	1633
BstZI	CGGCCG	1 cut(s)	110
BsuI	GTATCC	1 cut(s)	2066
BsuRI	GGCC	9 cut(s)	53, 65, 112, 251, 302, 348, 698, 748, 1029
BtgI	CCRYGG	3 cut(s)	107, 967, 1684
BtgZI	GCGATG	2 cut(s)	215, 295
BtsCI	GGATG	5 cut(s)	180, 834, 939, 1372, 2146
BtsI	GCAGTG	1 cut(s)	1242
BtsIMutI	CAGTG	7 cut(s)	399, 729, 873, 1242, 1335, 2315, 2324
Cac8I	GCNNGC	5 cut(s)	75, 284, 711, 1221, 1908
CciI	TCATGA	2 cut(s)	1432, 2160
CfoI	GCGC	5 cut(s)	6, 94, 369, 1338, 2221
Cfr10I	RCCGGY	2 cut(s)	73, 363
CseI	GACGC	1 cut(s)	2018
Csp6I	GTAC	7 cut(s)	166, 220, 241, 358, 1824, 2261, 2302
CviQI	GTAC	7 cut(s)	166, 220, 241, 358, 1824, 2261, 2302
DdeI	CTNAG	4 cut(s)	88, 1159, 1542, 1563
DpnI	GATC	9 cut(s)	485, 570, 778, 921, 1557, 1659, 1746, 2165, 2216
DpnII	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
DraI	TTTAAA	3 cut(s)	420, 1173, 1192
EaeI	YGGCCR	1 cut(s)	110
EagI	CGGCCG	1 cut(s)	110
Eam1104I	CTCTTC	3 cut(s)	540, 2028, 2058
EarI	CTCTTC	3 cut(s)	540, 2028, 2058
EciI	GGCGGA	4 cut(s)	14, 26, 1735, 1797
EclXI	CGGCCG	1 cut(s)	110
Eco130I	CCWWGG	3 cut(s)	894, 967, 1684
Eco24I	GRGCYC	2 cut(s)	55, 350
Eco31I	GGTCTC	2 cut(s)	94, 1481
Eco32I	GATATC	1 cut(s)	995
Eco47I	GGWCC	3 cut(s)	160, 1304, 1755
Eco52I	CGGCCG	1 cut(s)	110
Eco57I	CTGAAG	1 cut(s)	1932
Eco72I	CACGTG	1 cut(s)	153
Eco88I	CYCGRG	1 cut(s)	125
EcoO109I	RGGNCCY	3 cut(s)	160, 346, 347
EcoRI	GAATTC	2 cut(s)	640, 1837
EcoRII	CCWGG	5 cut(s)	210, 350, 1300, 1666, 2077
EcoRV	GATATC	1 cut(s)	995
EcoT14I	CCWWGG	3 cut(s)	894, 967, 1684
EcoT38I	GRGCYC	2 cut(s)	55, 350
ErhI	CCWWGG	3 cut(s)	894, 967, 1684
FalI	AAGNNNNNCTT	2 cut(s)	981, 1013
FaqI	GGGAC	1 cut(s)	2096
FauI	CCCGC	1 cut(s)	2214
FblI	GTMKAC	1 cut(s)	1632
Fnu4HI	GCNGC	7 cut(s)	113, 116, 206, 746, 915, 1983, 2052
FokI	GGATG	5 cut(s)	187, 821, 946, 1379, 2153
FriOI	GRGCYC	2 cut(s)	55, 350
Fsp4HI	GCNGC	7 cut(s)	113, 116, 206, 746, 915, 1983, 2052
FspBI	CTAG	2 cut(s)	410, 1067
GlaI	GCGC	5 cut(s)	5, 93, 368, 1337, 2220
GluI	GCNGC	7 cut(s)	113, 116, 206, 746, 915, 1983, 2052
GsaI	CCCAGC	1 cut(s)	346
GsuI	CTGGAG	5 cut(s)	260, 272, 1950, 2291, 2348
HaeIII	GGCC	9 cut(s)	53, 65, 112, 251, 302, 348, 698, 748, 1029
HapII	CCGG	8 cut(s)	74, 364, 403, 456, 566, 930, 1364, 2258
HgaI	GACGC	1 cut(s)	2018
HhaI	GCGC	5 cut(s)	6, 94, 369, 1338, 2221
Hin1I	GRCGYC	1 cut(s)	887
Hin6I	GCGC	5 cut(s)	4, 92, 367, 1336, 2219
HinP1I	GCGC	5 cut(s)	4, 92, 367, 1336, 2219
HincII	GTYRAC	3 cut(s)	832, 1035, 1879
HindII	GTYRAC	3 cut(s)	832, 1035, 1879
HindIII	AAGCTT	1 cut(s)	1904
HinfI	GANTC	9 cut(s)	459, 515, 799, 1070, 1935, 2201, 2318, 2330, 2357
HpaI	GTTAAC	1 cut(s)	832
HpaII	CCGG	8 cut(s)	74, 364, 403, 456, 566, 930, 1364, 2258
HphI	GGTGA	6 cut(s)	127, 1247, 1290, 1344, 1709, 2362
Hpy166II	GTNNAC	9 cut(s)	243, 832, 1008, 1035, 1580, 1633, 1824, 1879, 2231
Hpy188I	TCNGA	7 cut(s)	295, 319, 556, 839, 1534, 1564, 1662
Hpy8I	GTNNAC	9 cut(s)	243, 832, 1008, 1035, 1580, 1633, 1824, 1879, 2231
Hpy99I	CGWCG	2 cut(s)	428, 889
HpyAV	CCTTC	9 cut(s)	148, 347, 950, 1172, 1283, 1748, 1812, 1987, 2131
HpyCH4III	ACNGT	2 cut(s)	868, 1627
HpyCH4IV	ACGT	4 cut(s)	152, 887, 1101, 2107
HpyCH4V	TGCA	9 cut(s)	264, 650, 871, 914, 1240, 1985, 2207, 2313, 2336
HpyF10VI	GCNNNNNNNGC	5 cut(s)	112, 121, 270, 279, 715
HpyF3I	CTNAG	4 cut(s)	88, 1159, 1542, 1563
HpySE526I	ACGT	4 cut(s)	152, 887, 1101, 2107
Hsp92I	GRCGYC	1 cut(s)	887
HspAI	GCGC	5 cut(s)	4, 92, 367, 1336, 2219
Kpn2I	TCCGGA	1 cut(s)	455
KpnI	GGTACC	1 cut(s)	2305
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
KspAI	GTTAAC	1 cut(s)	832
Kzo9I	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
LmnI	GCTCC	3 cut(s)	226, 291, 1688
Lsp1109I	GCAGC	3 cut(s)	926, 1969, 2038
MaeI	CTAG	2 cut(s)	410, 1067
MaeII	ACGT	4 cut(s)	152, 887, 1101, 2107
MaeIII	GTNAC	5 cut(s)	608, 727, 862, 1204, 2108
MalI	GATC	9 cut(s)	485, 570, 778, 921, 1557, 1659, 1746, 2165, 2216
MboI	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
MhlI	GDGCHC	3 cut(s)	55, 350, 399
MlyI	GAGTC	3 cut(s)	1929, 2327, 2339
MmeI	TCCRAC	3 cut(s)	342, 862, 1733
MroI	TCCGGA	1 cut(s)	455
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	2 cut(s)	1103, 1462
MseI	TTAA	7 cut(s)	419, 831, 1172, 1191, 1977, 2268, 2273
MslI	CAYNNNNRTG	2 cut(s)	1683, 1812
MspA1I	CMGCKG	3 cut(s)	917, 2051, 2156
MspI	CCGG	8 cut(s)	74, 364, 403, 456, 566, 930, 1364, 2258
MspR9I	CCNGG	6 cut(s)	212, 352, 931, 1302, 1668, 2079
MssI	GTTTAAAC	1 cut(s)	420
MvaI	CCWGG	5 cut(s)	212, 352, 1302, 1668, 2079
MvnI	CGCG	3 cut(s)	369, 2219, 2221
MwoI	GCNNNNNNNGC	5 cut(s)	112, 121, 270, 279, 715
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	1 cut(s)	931
NcoI	CCATGG	2 cut(s)	967, 1684
NdeII	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
NgoMIV	GCCGGC	1 cut(s)	73
NmuCI	GTSAC	5 cut(s)	608, 727, 862, 1204, 2108
NspV	TTCGAA	1 cut(s)	683
PagI	TCATGA	2 cut(s)	1432, 2160
PasI	CCCWGGG	1 cut(s)	2078
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	2 cut(s)	1103, 1462
PfeI	GAWTC	6 cut(s)	459, 515, 799, 1070, 2201, 2357
PfoI	TCCNGGA	1 cut(s)	929
PkrI	GCNGC	7 cut(s)	114, 117, 207, 747, 916, 1984, 2053
PleI	GAGTC	3 cut(s)	1929, 2326, 2338
PmaCI	CACGTG	1 cut(s)	153
PmeI	GTTTAAAC	1 cut(s)	420
PmlI	CACGTG	1 cut(s)	153
PpsI	GAGTC	3 cut(s)	1929, 2326, 2338
Ppu21I	YACGTR	2 cut(s)	153, 2108
PpuMI	RGGWCCY	1 cut(s)	160
PsiI	TTATAA	2 cut(s)	1517, 2345
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	5 cut(s)	210, 350, 1300, 1666, 2077
PspCI	CACGTG	1 cut(s)	153
PspFI	CCCAGC	1 cut(s)	342
PspGI	CCWGG	5 cut(s)	210, 350, 1300, 1666, 2077
PspOMI	GGGCCC	2 cut(s)	51, 346
PspPPI	RGGWCCY	1 cut(s)	160
PvuII	CAGCTG	3 cut(s)	917, 2051, 2156
RsaI	GTAC	7 cut(s)	167, 221, 242, 359, 1825, 2262, 2303
RsaNI	GTAC	7 cut(s)	166, 220, 241, 358, 1824, 2261, 2302
RseI	CAYNNNNRTG	2 cut(s)	1683, 1812
SaqAI	TTAA	7 cut(s)	419, 831, 1172, 1191, 1977, 2268, 2273
SatI	GCNGC	7 cut(s)	113, 116, 206, 746, 915, 1983, 2052
Sau3AI	GATC	9 cut(s)	483, 568, 776, 919, 1555, 1657, 1744, 2163, 2214
SchI	GAGTC	3 cut(s)	1929, 2327, 2339
ScrFI	CCNGG	6 cut(s)	212, 352, 931, 1302, 1668, 2079
SduI	GDGCHC	3 cut(s)	55, 350, 399
SfuI	TTCGAA	1 cut(s)	683
SinI	GGWCC	3 cut(s)	160, 1304, 1755
SmiMI	CAYNNNNRTG	2 cut(s)	1683, 1812
SmlI	CTYRAG	1 cut(s)	977
SmoI	CTYRAG	1 cut(s)	977
SspI	AATATT	1 cut(s)	1426
SspMI	CTAG	2 cut(s)	410, 1067
StyD4I	CCNGG	6 cut(s)	210, 350, 929, 1300, 1666, 2077
StyI	CCWWGG	3 cut(s)	894, 967, 1684
TaaI	ACNGT	2 cut(s)	868, 1627
TaiI	ACGT	4 cut(s)	155, 890, 1104, 2110
TaqI	TCGA	4 cut(s)	179, 462, 486, 683
TatI	WGTACW	4 cut(s)	219, 240, 357, 1823
TauI	GCSGC	4 cut(s)	115, 118, 208, 748
TfiI	GAWTC	6 cut(s)	459, 515, 799, 1070, 2201, 2357
Tru1I	TTAA	7 cut(s)	419, 831, 1172, 1191, 1977, 2268, 2273
Tru9I	TTAA	7 cut(s)	419, 831, 1172, 1191, 1977, 2268, 2273
TscAI	CASTG	7 cut(s)	399, 736, 873, 1242, 1335, 2315, 2331
TseFI	GTSAC	5 cut(s)	608, 727, 862, 1204, 2108
TseI	GCWGC	3 cut(s)	914, 1982, 2051
Tsp45I	GTSAC	5 cut(s)	608, 727, 862, 1204, 2108
TspGWI	ACGGA	1 cut(s)	260
TspRI	CASTG	7 cut(s)	399, 736, 873, 1242, 1335, 2315, 2331
VpaK11BI	GGWCC	3 cut(s)	160, 1304, 1755
XapI	RAATTY	8 cut(s)	634, 640, 685, 850, 1495, 1510, 1837, 1868
XbaI	TCTAGA	1 cut(s)	1066
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1691
XmiI	GTMKAC	1 cut(s)	1632
XmnI	GAANNNNTTC	2 cut(s)	1103, 1462
XspI	CTAG	2 cut(s)	410, 1067
ZraI	GACGTC	1 cut(s)	888

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.