FvH4_1g19424

function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0018354
Physical Location & Seq
Forward (+)
11697673 .. 11705863
8191 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19424.t1

Sequence Viewer

Length: 588 bp
ATGGACCACCTGGATTTCAACAAGGATCAAGGCAGCAACCATACCAACAAGCTTCACCTCAACAAGCTTCAAGGGCAGTCTATCAAGAACTTGGAGAGACAAGTTGGGCAACTTGCAACGGCGTTGCAAACAAGACCTCAAGGTGTTCTTCCTAGCACAACAGTTCAAAATCCTAAAGATGGGAGCATGGCTGAGCTTAATGCAGTCACTTTGAGAAGTGGAAAACAATTAGAGGAGCCTACTAAGCCTCACAAGATGTCCAAGAGTGGTCAAGCTGAGAAGTATGAGCTGGAAATAAAGGAAGGCGAAAAGAGCAAAACTAGGCAGAAAGAGATCGATCCTTCAGACCCAAAACAAAATCTTCAAGTTTCTTCTCCTTCCAAGGACGTCCGCACTGGGCTTAAAGTGCGGATATCCCTCCGTTCTCCACCGGACGTCTCCAACGACGACGCCCCTCCACCCGAGCGGACTCCAGCGGCATCCTGTTCACTTTCTCTTCCCGGCGAGATCAATTTGAAGTCCGCTCAGGTGGAGGCGCGCCATCGTCGGCGCCGGACGGTGAAGAACGGAGGGACGTCAATACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asp_protease_2 Retrotrans_gag

Protein Analysis

196

Amino Acids

21.48

Weight (kDa)

9.74

Isoelectric Point (pI)

63.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 390, 438, 578
AccB1I GGYRCC 1 cut(s) 549
AccBSI CCGCTC 2 cut(s) 466, 524
AccII CGCG 1 cut(s) 538
AciI CCGC 5 cut(s) 391, 409, 466, 476, 522
AclWI GGATC 2 cut(s) 33, 332
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 5 cut(s) 387, 435, 450, 550, 575
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 5 cut(s) 19, 71, 167, 365, 517
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 5 cut(s) 52, 67, 196, 275, 289
AluI AGCT 5 cut(s) 52, 67, 196, 275, 289
Alw26I GTCTC 2 cut(s) 91, 442
AlwI GGATC 2 cut(s) 33, 332
Ama87I CYCGRG 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 33
AscI GGCGCGCC 1 cut(s) 536
AspLEI GCGC 3 cut(s) 538, 540, 552
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 2 cut(s) 47, 571
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 173, 549
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 1 cut(s) 11
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 91, 442
BfaI CTAG 2 cut(s) 153, 321
BfoI RGCGCY 1 cut(s) 553
BisI GCNGC 2 cut(s) 34, 477
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 2 cut(s) 35, 478
Bme1390I CCNGG 2 cut(s) 11, 501
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 237, 551
BmrFI CCNGG 2 cut(s) 11, 501
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 1 cut(s) 488
BmuI ACTGGG 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 501
Bsa29I ATCGAT 1 cut(s) 336
BsaHI GRCGYC 5 cut(s) 387, 435, 450, 550, 575
BsaJI CCNNGG 1 cut(s) 381
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 11
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 479
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 3 cut(s) 183, 267, 539
BseNI ACTGG 1 cut(s) 400
BsePI GCGCGC 1 cut(s) 536
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 538
BshNI GGYRCC 1 cut(s) 549
BshVI ATCGAT 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 431, 501, 553
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 91, 442
BsmBI CGTCTC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 461
Bsp143I GATC 4 cut(s) 25, 333, 337, 507
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 5 cut(s) 391, 409, 466, 476, 522
BspCNI CTCAG 3 cut(s) 184, 268, 538
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 237, 551
BspPI GGATC 2 cut(s) 33, 332
BspT107I GGYRCC 1 cut(s) 549
BsrBI CCGCTC 2 cut(s) 466, 524
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 381
BssHII GCGCGC 1 cut(s) 536
BssMI GATC 4 cut(s) 25, 333, 337, 507
BssNI GRCGYC 5 cut(s) 387, 435, 450, 550, 575
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 2 cut(s) 163, 559
Bst6I CTCTTC 1 cut(s) 501
BstACI GRCGYC 5 cut(s) 387, 435, 450, 550, 575
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 4 cut(s) 192, 243, 276, 525
BstF5I GGATG 1 cut(s) 479
BstFNI CGCG 1 cut(s) 538
BstH2I RGCGCY 1 cut(s) 553
BstHHI GCGC 3 cut(s) 538, 540, 552
BstKTI GATC 4 cut(s) 28, 336, 340, 510
BstMAI GTCTC 2 cut(s) 91, 442
BstMBI GATC 4 cut(s) 25, 333, 337, 507
BstMWI GCNNNNNNNGC 4 cut(s) 73, 244, 312, 406
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 9, 499
BstUI CGCG 1 cut(s) 538
BstV1I GCAGC 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 336
BtsCI GGATG 1 cut(s) 479
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 538
CfoI GCGC 3 cut(s) 538, 540, 552
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 1 cut(s) 458
CviAII CATG 1 cut(s) 187
CviJI RGCY 9 cut(s) 52, 67, 191, 196, 238, 247, 275, 289, 400
CviKI_1 RGCY 9 cut(s) 52, 67, 191, 196, 238, 247, 275, 289, 400
DdeI CTNAG 4 cut(s) 192, 243, 276, 525
DinI GGCGCC 1 cut(s) 551
DpnI GATC 4 cut(s) 27, 335, 339, 509
DpnII GATC 4 cut(s) 25, 333, 337, 507
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 381
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 9
EcoRV GATATC 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 381
EgeI GGCGCC 1 cut(s) 551
EheI GGCGCC 1 cut(s) 551
ErhI CCWWGG 1 cut(s) 381
Esp3I CGTCTC 1 cut(s) 442
FaeI CATG 1 cut(s) 190
FaiI YATR 3 cut(s) 42, 188, 285
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FatI CATG 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 34, 477
FokI GGATG 1 cut(s) 466
Fsp4HI GCNGC 2 cut(s) 34, 477
FspBI CTAG 2 cut(s) 153, 321
GlaI GCGC 3 cut(s) 537, 539, 551
GluI GCNGC 2 cut(s) 34, 477
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 553
HapII CCGG 3 cut(s) 431, 501, 553
HgaI GACGC 1 cut(s) 458
HhaI GCGC 3 cut(s) 538, 540, 552
Hin1I GRCGYC 5 cut(s) 387, 435, 450, 550, 575
Hin1II CATG 1 cut(s) 190
Hin6I GCGC 3 cut(s) 536, 538, 550
HinP1I GCGC 3 cut(s) 536, 538, 550
HindIII AAGCTT 2 cut(s) 50, 65
HinfI GANTC 1 cut(s) 469
HpaII CCGG 3 cut(s) 431, 501, 553
HphI GGTGA 2 cut(s) 47, 571
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 1 cut(s) 488
Hpy99I CGWCG 3 cut(s) 449, 452, 549
HpyAV CCTTC 3 cut(s) 296, 351, 387
HpyCH4III ACNGT 2 cut(s) 163, 559
HpyCH4IV ACGT 3 cut(s) 387, 435, 575
HpyCH4V TGCA 3 cut(s) 116, 127, 203
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 244, 312, 406
HpyF3I CTNAG 4 cut(s) 192, 243, 276, 525
HpySE526I ACGT 3 cut(s) 387, 435, 575
Hsp92I GRCGYC 5 cut(s) 387, 435, 450, 550, 575
Hsp92II CATG 1 cut(s) 190
HspAI GCGC 3 cut(s) 536, 538, 550
KasI GGCGCC 1 cut(s) 549
Kzo9I GATC 4 cut(s) 25, 333, 337, 507
LmnI GCTCC 2 cut(s) 183, 235
LpnPI CCDG 9 cut(s) 23, 275, 381, 444, 486, 496, 512, 514, 566
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 488
MaeI CTAG 2 cut(s) 153, 321
MaeII ACGT 3 cut(s) 387, 435, 575
MaeIII GTNAC 1 cut(s) 205
MalI GATC 4 cut(s) 27, 335, 339, 509
MbiI CCGCTC 2 cut(s) 466, 524
MboI GATC 4 cut(s) 25, 333, 337, 507
MboII GAAGA 5 cut(s) 140, 353, 363, 488, 574
MluCI AATT 2 cut(s) 227, 511
Mly113I GGCGCC 1 cut(s) 550
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 8 cut(s) 68, 147, 226, 258, 428, 465, 526, 563
MseI TTAA 3 cut(s) 198, 402, 586
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 3 cut(s) 431, 501, 553
MspR9I CCNGG 2 cut(s) 11, 501
MvaI CCWGG 1 cut(s) 11
MvnI CGCG 1 cut(s) 538
MwoI GCNNNNNNNGC 4 cut(s) 73, 244, 312, 406
NarI GGCGCC 1 cut(s) 550
NciI CCSGG 1 cut(s) 501
NdeII GATC 4 cut(s) 25, 333, 337, 507
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 2 cut(s) 237, 551
NmuCI GTSAC 1 cut(s) 205
PalAI GGCGCGCC 1 cut(s) 536
PauI GCGCGC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PkrI GCNGC 2 cut(s) 35, 478
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 237, 551
PspPI GGNCC 1 cut(s) 4
PteI GCGCGC 1 cut(s) 536
SaqAI TTAA 3 cut(s) 198, 402, 586
SatI GCNGC 2 cut(s) 34, 477
Sau3AI GATC 4 cut(s) 25, 333, 337, 507
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 2 cut(s) 11, 501
SfaNI GCATC 1 cut(s) 488
SfoI GGCGCC 1 cut(s) 551
SgsI GGCGCGCC 1 cut(s) 536
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 2 cut(s) 227, 511
SsiI CCGC 5 cut(s) 391, 409, 466, 476, 522
SspDI GGCGCC 1 cut(s) 549
SspMI CTAG 2 cut(s) 153, 321
StyD4I CCNGG 2 cut(s) 9, 499
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 163, 559
TaiI ACGT 3 cut(s) 390, 438, 578
TaqI TCGA 1 cut(s) 336
TasI AATT 2 cut(s) 227, 511
TauI GCSGC 1 cut(s) 479
Tru1I TTAA 3 cut(s) 198, 402, 586
Tru9I TTAA 3 cut(s) 198, 402, 586
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 1 cut(s) 205
TspGWI ACGGA 2 cut(s) 410, 582
TspRI CASTG 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 4
XspI CTAG 2 cut(s) 153, 321
ZraI GACGTC 3 cut(s) 388, 436, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.