Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

11887303 .. 11895390

Gene Structure 8088 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g19670.t7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2313 bp

ATGGTCCTCGCTGGTTCATTGCAATTATCCCATGGATTGGGACTTTGCAAGAATCAGGGGTGCCATAAACAGTTTAAGGTACTACAAGACTGCTGTTTTTTTTTATTGTCAATTTCCATATCAGTGTTATCCTGTAATTGCTTTGTGATAAGTAAATTTATTCTCTTACAGAGGGGAATAAGATGGGACAAATTACATCTCTCAAGCACTGCCCTTATGTCACGAGTCTCGTTTCAGAGAACAGAGTCTTGGAGCATTGGTTTATCAGAGAATGTATACAGGTCTATACATACCTCATCTCACAGATATCATGCATTTAAATGCCATTCTTCTGTAGCACCAGGCCAACCGTTTGGCCAACATGGCTTGGAGACTGCTACTATGGTGTTGAAAAGGTCATATGATCTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCGTCATTGTCTTTGCCATTTGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATACAGGCTGGAAAAAGAGTAACACACACTATGTTACAAACTCGTATATTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATTTGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCCAGTCAGGTCGTTAAGCAACGGCTTTTGGACTTTATAAGATCGCTGTCAACTGTTCTCTCTTTTGCCTATTGTCTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCCGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTGACTGCAGGAGGTCTTGGGACTGTCTTATTGACTCTGGCTGGCCGTGAGATATTTACAAATTTCCTGTCAAGTGCGATGATTCATGCCACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACGGTTGAGCATGTAGGCTGGTGGTCGCCAACTATTGTAAGAGGTGAAGATCGTGAAGCTGTACACATCCCAAACCATAAGTTCACAGTGAATGTTGTCAGAAATCTCACTCAAAAAACTCATTGGCGCATCAAAACTCACCTTGCCATTAGTCATTTGGATGTTCATAAGATAAATAATATTGTTGCCGATATGCGTAAAGTCTTGTCCAAGAATCCCCAAGTTGAACAGCAGAGGTTGCATAGAAGAGTGTTTCTGGAAAATGTTACTCCTGAAAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACATCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAATGATACTGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCATGGTGGAGTATCCCCTAGACGCCCGTTGTTGCTTATTGAACCTTCATACAAGATAAATGGAGAAGATAAAAAAAGAAGTCGCTCTGGGCAGACCAGTGGAGAGCGAGATGGGAAGGCAACAGTGCGGCCATCACCTGACCCCAAAGTGGATGCTAAAGTTGGGGACTCCAAGACCAGTGAAACACTAACATCTGACACCAAGGGAGATGCCAGGACGCCAAATCCAATCCCCAAGGACGACACCAATGCTTCAAAGCCATCTCCTTCTGATCCCAAAGTAGGTGATAAAGGAACAATCAAGTCAAGGTCAAACTCCAAGACAAGTTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCAGACTTAATATCTGATCATTTGACAGAGAAGATGTCTGGCAGAAAGCAAGTGAATAATGCAAATGGAAATGTTTTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCTTCTGCAAGTGGTGCTGACAAAACTAGTGGATCAGTGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGCTGCGGAGCCACCAACAACAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGAAATGGCTTCTGCTTCATCTCACGCAGATGCAAATGATTTGGCTTCTGTCCGGAAAGGTAATGCGTCTTCAAATACTGAGAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

771

Amino Acids

84.9

Weight (kDa)

9.25

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	149 - 278	3.8e-72	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	280 - 350	1.8e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	355 - 441	1.1e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	686
Acc36I	ACCTGC	1 cut(s)	2110
AccB1I	GGYRCC	1 cut(s)	60
AccB7I	CCANNNNNTGG	2 cut(s)	37, 449
AccI	GTMKAC	1 cut(s)	276
AccIII	TCCGGA	1 cut(s)	2223
AciI	CCGC	4 cut(s)	985, 1511, 1654, 2081
AclWI	GGATC	6 cut(s)	620, 633, 730, 1416, 1793, 2041
AcoI	YGGCCR	3 cut(s)	355, 934, 1655
AcsI	RAATTY	3 cut(s)	155, 752, 952
AcyI	GRCGYC	3 cut(s)	453, 1549, 1745
AfaI	GTAC	4 cut(s)	81, 824, 1107, 1462
AfiI	CCNNNNNNNGG	7 cut(s)	37, 449, 595, 888, 1435, 1675, 1808
AgsI	TTSAA	8 cut(s)	391, 433, 578, 1013, 1271, 1568, 1782, 2244
AhlI	ACTAGT	1 cut(s)	2027
AjnI	CCWGG	3 cut(s)	340, 1739, 2287
AjuI	GAANNNNNNNTTGG	2 cut(s)	232, 264
AluBI	AGCT	6 cut(s)	818, 1103, 1328, 1397, 2078, 2282
AluI	AGCT	6 cut(s)	818, 1103, 1328, 1397, 2078, 2282
Alw21I	GWGCWC	1 cut(s)	1979
Alw26I	GTCTC	3 cut(s)	232, 365, 482
AlwI	GGATC	6 cut(s)	620, 633, 730, 1416, 1793, 2041
Aor13HI	TCCGGA	1 cut(s)	2223
AoxI	GGCC	5 cut(s)	343, 355, 934, 1437, 1655
ApeKI	GCWGC	4 cut(s)	842, 1892, 2078, 2282
ApoI	RAATTY	3 cut(s)	155, 752, 952
ArsI	GACNNNNNNTTYG	2 cut(s)	944, 976
Asp700I	GAANNNNTTC	1 cut(s)	1008
AspLEI	GCGC	1 cut(s)	1173
AspS9I	GGNCC	2 cut(s)	4, 1438
AsuHPI	GGTGA	5 cut(s)	1100, 1175, 1653, 1823, 2075
AvaII	GGWCC	1 cut(s)	4
BalI	TGGCCA	1 cut(s)	357
BamHI	GGATCC	1 cut(s)	625
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	2147
BarI	GAAGNNNNNNTAC	2 cut(s)	2224, 2256
BauI	CACGAG	1 cut(s)	222
BbsI	GAAGAC	2 cut(s)	1364, 2232
Bbv12I	GWGCWC	1 cut(s)	1979
BbvI	GCAGC	4 cut(s)	829, 1879, 2065, 2269
BccI	CCATC	6 cut(s)	177, 451, 1437, 1631, 1666, 1795
BceAI	ACGGC	3 cut(s)	686, 815, 921
BcgI	CGANNNNNNTGC	2 cut(s)	1757, 1791
BciT130I	CCWGG	3 cut(s)	342, 1741, 2289
BciVI	GTATCC	1 cut(s)	1549
BclI	TGATCA	2 cut(s)	1909, 2269
BcoDI	GTCTC	3 cut(s)	232, 365, 482
BcuI	ACTAGT	1 cut(s)	2027
BfaI	CTAG	5 cut(s)	869, 1545, 1983, 2028, 2108
BfmI	CTRYAG	4 cut(s)	333, 642, 897, 1893
BfuAI	ACCTGC	1 cut(s)	2110
BfuI	GTATCC	1 cut(s)	1549
BglI	GCCNNNNNGGC	1 cut(s)	363
BisI	GCNGC	5 cut(s)	843, 1655, 1893, 2079, 2283
BlsI	GCNGC	5 cut(s)	844, 1656, 1894, 2080, 2284
Bme1390I	CCNGG	3 cut(s)	342, 1741, 2289
Bme18I	GGWCC	1 cut(s)	4
BmgT120I	GGNCC	2 cut(s)	4, 1438
BmiI	GGNNCC	4 cut(s)	62, 441, 627, 2085
BmrFI	CCNGG	3 cut(s)	342, 1741, 2289
BmrI	ACTGGG	1 cut(s)	2042
BmsI	GCATC	6 cut(s)	1182, 1669, 1726, 1852, 2061, 2191
BmuI	ACTGGG	1 cut(s)	2042
BpiI	GAAGAC	2 cut(s)	1364, 2232
BplI	GAGNNNNNCTC	2 cut(s)	591, 623
BpuEI	CTTGAG	1 cut(s)	187
BsaHI	GRCGYC	3 cut(s)	453, 1549, 1745
BsaI	GGTCTC	1 cut(s)	482
BsaJI	CCNNGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
BsaWI	WCCGGW	1 cut(s)	2223
BsaXI	ACNNNNNCTCC	4 cut(s)	244, 274, 570, 600
Bsc4I	CCNNNNNNNGG	7 cut(s)	37, 449, 595, 888, 1435, 1675, 1808
Bse1I	ACTGG	6 cut(s)	629, 651, 777, 1623, 1704, 2048
Bse3DI	GCAATG	2 cut(s)	17, 609
BseAI	TCCGGA	1 cut(s)	2223
BseBI	CCWGG	3 cut(s)	342, 1741, 2289
BseDI	CCNNGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
BseGI	GGATG	4 cut(s)	1110, 1210, 1684, 2042
BseLI	CCNNNNNNNGG	7 cut(s)	37, 449, 595, 888, 1435, 1675, 1808
BseMI	GCAATG	2 cut(s)	17, 609
BseMII	CTCAG	2 cut(s)	488, 2241
BseNI	ACTGG	6 cut(s)	629, 651, 777, 1623, 1704, 2048
BseXI	GCAGC	4 cut(s)	829, 1879, 2065, 2269
BseYI	CCCAGC	1 cut(s)	809
BsgI	GTGCAG	2 cut(s)	1487, 2268
BshFI	GGCC	5 cut(s)	345, 357, 936, 1439, 1657
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1979
BsiSI	CCGG	1 cut(s)	2224
BslFI	GGGAC	4 cut(s)	54, 200, 925, 1706
BslI	CCNNNNNNNGG	7 cut(s)	37, 449, 595, 888, 1435, 1675, 1808
BsmAI	GTCTC	3 cut(s)	232, 365, 482
BsmFI	GGGAC	4 cut(s)	54, 200, 925, 1706
BsnI	GGCC	5 cut(s)	345, 357, 936, 1439, 1657
Bso31I	GGTCTC	1 cut(s)	482
Bsp1286I	GDGCHC	2 cut(s)	1979, 2147
Bsp13I	TCCGGA	1 cut(s)	2223
Bsp1407I	TGTACA	1 cut(s)	1105
Bsp19I	CCATGG	1 cut(s)	31
BspACI	CCGC	4 cut(s)	985, 1511, 1654, 2081
BspANI	GGCC	5 cut(s)	345, 357, 936, 1439, 1657
BspCNI	CTCAG	2 cut(s)	489, 2242
BspEI	TCCGGA	1 cut(s)	2223
BspHI	TCATGA	2 cut(s)	1332, 1369
BspLI	GGNNCC	4 cut(s)	62, 441, 627, 2085
BspMAI	CTGCAG	2 cut(s)	901, 1897
BspMI	ACCTGC	1 cut(s)	2110
BspPI	GGATC	6 cut(s)	620, 633, 730, 1416, 1793, 2041
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	1 cut(s)	482
BsrDI	GCAATG	2 cut(s)	17, 609
BsrGI	TGTACA	1 cut(s)	1105
BsrI	ACTGG	6 cut(s)	629, 651, 777, 1623, 1704, 2048
BssECI	CCNNGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
BssNAI	GTATAC	1 cut(s)	277
BssNI	GRCGYC	3 cut(s)	453, 1549, 1745
BssSI	CACGAG	1 cut(s)	222
BssT1I	CCWWGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
Bst1107I	GTATAC	1 cut(s)	277
Bst2BI	CACGAG	1 cut(s)	222
Bst2UI	CCWGG	3 cut(s)	342, 1741, 2289
Bst4CI	ACNGT	9 cut(s)	72, 351, 703, 916, 1048, 1132, 1465, 1499, 1651
Bst6I	CTCTTC	3 cut(s)	1285, 1368, 2008
BstACI	GRCGYC	3 cut(s)	453, 1549, 1745
BstAPI	GCANNNNNTGC	2 cut(s)	1282, 2015
BstAUI	TGTACA	1 cut(s)	1105
BstC8I	GCNNGC	2 cut(s)	934, 2076
BstDEI	CTNAG	4 cut(s)	497, 515, 1415, 2250
BstDSI	CCRYGG	1 cut(s)	31
BstF5I	GGATG	4 cut(s)	1110, 1210, 1684, 2042
BstHHI	GCGC	1 cut(s)	1173
BstMAI	GTCTC	3 cut(s)	232, 365, 482
BstMWI	GCNNNNNNNGC	5 cut(s)	363, 815, 1282, 1445, 2015
BstNI	CCWGG	3 cut(s)	342, 1741, 2289
BstNSI	RCATGY	1 cut(s)	1058
BstSCI	CCNGG	3 cut(s)	340, 1739, 2287
BstSFI	CTRYAG	4 cut(s)	333, 642, 897, 1893
BstV1I	GCAGC	4 cut(s)	829, 1879, 2065, 2269
BstV2I	GAAGAC	2 cut(s)	1364, 2232
BstX2I	RGATCY	2 cut(s)	625, 1408
BstXI	CCANNNNNNTGG	2 cut(s)	353, 809
BstYI	RGATCY	2 cut(s)	625, 1408
BstZ17I	GTATAC	1 cut(s)	277
BsuI	GTATCC	1 cut(s)	1549
BsuRI	GGCC	5 cut(s)	345, 357, 936, 1439, 1657
BtgI	CCRYGG	1 cut(s)	31
BtgZI	GCGATG	1 cut(s)	983
BtsCI	GGATG	4 cut(s)	1110, 1210, 1684, 2042
BtsI	GCAGTG	1 cut(s)	207
BveI	ACCTGC	1 cut(s)	2110
Cac8I	GCNNGC	2 cut(s)	934, 2076
CciI	TCATGA	2 cut(s)	1332, 1369
CfoI	GCGC	1 cut(s)	1173
Cfr13I	GGNCC	2 cut(s)	4, 1438
CseI	GACGC	5 cut(s)	442, 482, 1557, 1753, 2226
Csp6I	GTAC	4 cut(s)	80, 823, 1106, 1461
CspCI	CAANNNNNGTGG	2 cut(s)	2011, 2046
CviQI	GTAC	4 cut(s)	80, 823, 1106, 1461
DdeI	CTNAG	4 cut(s)	497, 515, 1415, 2250
DraI	TTTAAA	1 cut(s)	319
EaeI	YGGCCR	3 cut(s)	355, 934, 1655
Eam1104I	CTCTTC	3 cut(s)	1285, 1368, 2008
EarI	CTCTTC	3 cut(s)	1285, 1368, 2008
Eco130I	CCWWGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
Eco24I	GRGCYC	1 cut(s)	2147
Eco31I	GGTCTC	1 cut(s)	482
Eco32I	GATATC	1 cut(s)	308
Eco47I	GGWCC	1 cut(s)	4
EcoRII	CCWGG	3 cut(s)	340, 1739, 2287
EcoRV	GATATC	1 cut(s)	308
EcoT14I	CCWWGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
EcoT22I	ATGCAT	1 cut(s)	316
EcoT38I	GRGCYC	1 cut(s)	2147
ErhI	CCWWGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
FaqI	GGGAC	4 cut(s)	54, 200, 925, 1706
FauI	CCCGC	1 cut(s)	992
FauNDI	CATATG	1 cut(s)	400
FbaI	TGATCA	2 cut(s)	1909, 2269
FblI	GTMKAC	1 cut(s)	276
Fnu4HI	GCNGC	5 cut(s)	843, 1655, 1893, 2079, 2283
FokI	GGATG	4 cut(s)	1097, 1217, 1691, 2029
FriOI	GRGCYC	1 cut(s)	2147
Fsp4HI	GCNGC	5 cut(s)	843, 1655, 1893, 2079, 2283
FspBI	CTAG	5 cut(s)	869, 1545, 1983, 2028, 2108
GlaI	GCGC	1 cut(s)	1172
GluI	GCNGC	5 cut(s)	843, 1655, 1893, 2079, 2283
GsaI	CCCAGC	1 cut(s)	813
HaeIII	GGCC	5 cut(s)	345, 357, 936, 1439, 1657
HapII	CCGG	1 cut(s)	2224
HgaI	GACGC	5 cut(s)	442, 482, 1557, 1753, 2226
HhaI	GCGC	1 cut(s)	1173
Hin1I	GRCGYC	3 cut(s)	453, 1549, 1745
Hin6I	GCGC	1 cut(s)	1171
HinP1I	GCGC	1 cut(s)	1171
HincII	GTYRAC	1 cut(s)	699
HindII	GTYRAC	1 cut(s)	699
HpaII	CCGG	1 cut(s)	2224
HphI	GGTGA	5 cut(s)	1100, 1175, 1653, 1823, 2075
Hpy166II	GTNNAC	5 cut(s)	277, 699, 823, 1108, 1128
Hpy188III	TCNNGA	9 cut(s)	222, 1097, 1301, 1316, 1333, 1339, 1370, 2108, 2224
Hpy8I	GTNNAC	5 cut(s)	277, 699, 823, 1108, 1128
HpyAV	CCTTC	7 cut(s)	1019, 1581, 1636, 1803, 2054, 2168, 2248
HpyCH4III	ACNGT	9 cut(s)	72, 351, 703, 916, 1048, 1132, 1465, 1499, 1651
HpyF10VI	GCNNNNNNNGC	5 cut(s)	363, 815, 1282, 1445, 2015
HpyF3I	CTNAG	4 cut(s)	497, 515, 1415, 2250
Hsp92I	GRCGYC	3 cut(s)	453, 1549, 1745
HspAI	GCGC	1 cut(s)	1171
Kpn2I	TCCGGA	1 cut(s)	2223
Ksp22I	TGATCA	2 cut(s)	1909, 2269
LmnI	GCTCC	4 cut(s)	252, 591, 599, 2083
Lsp1109I	GCAGC	4 cut(s)	829, 1879, 2065, 2269
LweI	GCATC	6 cut(s)	1182, 1669, 1726, 1852, 2061, 2191
MaeI	CTAG	5 cut(s)	869, 1545, 1983, 2028, 2108
MaeIII	GTNAC	6 cut(s)	219, 545, 559, 767, 1309, 2038
MflI	RGATCY	2 cut(s)	625, 1408
MhlI	GDGCHC	2 cut(s)	1979, 2147
MlsI	TGGCCA	1 cut(s)	357
MluCI	AATT	9 cut(s)	23, 111, 136, 155, 191, 434, 752, 803, 952
MluNI	TGGCCA	1 cut(s)	357
MlyI	GAGTC	8 cut(s)	234, 254, 782, 919, 1428, 1508, 1688, 1877
MmeI	TCCRAC	1 cut(s)	763
Mox20I	TGGCCA	1 cut(s)	357
Mph1103I	ATGCAT	1 cut(s)	316
MroI	TCCGGA	1 cut(s)	2223
MroXI	GAANNNNTTC	1 cut(s)	1008
MscI	TGGCCA	1 cut(s)	357
MseI	TTAA	4 cut(s)	75, 318, 663, 1901
MslI	CAYNNNNRTG	4 cut(s)	122, 319, 1203, 2199
Msp20I	TGGCCA	1 cut(s)	357
MspA1I	CMGCKG	2 cut(s)	2078, 2282
MspI	CCGG	1 cut(s)	2224
MspR9I	CCNGG	3 cut(s)	342, 1741, 2289
MvaI	CCWGG	3 cut(s)	342, 1741, 2289
MwoI	GCNNNNNNNGC	5 cut(s)	363, 815, 1282, 1445, 2015
NcoI	CCATGG	1 cut(s)	31
NdeI	CATATG	1 cut(s)	400
NlaIV	GGNNCC	4 cut(s)	62, 441, 627, 2085
NmuCI	GTSAC	2 cut(s)	219, 2038
NsiI	ATGCAT	1 cut(s)	316
NspI	RCATGY	1 cut(s)	1058
PagI	TCATGA	2 cut(s)	1332, 1369
PdmI	GAANNNNTTC	1 cut(s)	1008
PfeI	GAWTC	5 cut(s)	52, 973, 1009, 1258, 1877
PflMI	CCANNNNNTGG	2 cut(s)	37, 449
PkrI	GCNGC	5 cut(s)	844, 1656, 1894, 2080, 2284
PleI	GAGTC	8 cut(s)	233, 253, 781, 919, 1427, 1508, 1688, 1877
PpsI	GAGTC	8 cut(s)	233, 253, 781, 919, 1427, 1508, 1688, 1877
PsiI	TTATAA	1 cut(s)	686
Psp6I	CCWGG	3 cut(s)	340, 1739, 2287
PspFI	CCCAGC	1 cut(s)	809
PspGI	CCWGG	3 cut(s)	340, 1739, 2287
PspN4I	GGNNCC	4 cut(s)	62, 441, 627, 2085
PspPI	GGNCC	2 cut(s)	4, 1438
PstI	CTGCAG	2 cut(s)	901, 1897
PsuI	RGATCY	2 cut(s)	625, 1408
PvuII	CAGCTG	2 cut(s)	2078, 2282
RsaI	GTAC	4 cut(s)	81, 824, 1107, 1462
RsaNI	GTAC	4 cut(s)	80, 823, 1106, 1461
RseI	CAYNNNNRTG	4 cut(s)	122, 319, 1203, 2199
SaqAI	TTAA	4 cut(s)	75, 318, 663, 1901
SatI	GCNGC	5 cut(s)	843, 1655, 1893, 2079, 2283
Sau96I	GGNCC	2 cut(s)	4, 1438
SchI	GAGTC	8 cut(s)	234, 254, 782, 919, 1428, 1508, 1688, 1877
ScrFI	CCNGG	3 cut(s)	342, 1741, 2289
SduI	GDGCHC	2 cut(s)	1979, 2147
SfaNI	GCATC	6 cut(s)	1182, 1669, 1726, 1852, 2061, 2191
SfcI	CTRYAG	4 cut(s)	333, 642, 897, 1893
SinI	GGWCC	1 cut(s)	4
SmiI	ATTTAAAT	1 cut(s)	319
SmiMI	CAYNNNNRTG	4 cut(s)	122, 319, 1203, 2199
SmlI	CTYRAG	1 cut(s)	202
SmoI	CTYRAG	1 cut(s)	202
SpeI	ACTAGT	1 cut(s)	2027
Sse9I	AATT	9 cut(s)	23, 111, 136, 155, 191, 434, 752, 803, 952
SsiI	CCGC	4 cut(s)	985, 1511, 1654, 2081
SspI	AATATT	1 cut(s)	1225
SspMI	CTAG	5 cut(s)	869, 1545, 1983, 2028, 2108
StyD4I	CCNGG	3 cut(s)	340, 1739, 2287
StyI	CCWWGG	6 cut(s)	31, 1728, 1761, 1887, 2125, 2136
SwaI	ATTTAAAT	1 cut(s)	319
TaaI	ACNGT	9 cut(s)	72, 351, 703, 916, 1048, 1132, 1465, 1499, 1651
TasI	AATT	9 cut(s)	23, 111, 136, 155, 191, 434, 752, 803, 952
TatI	WGTACW	2 cut(s)	822, 1105
TauI	GCSGC	1 cut(s)	1657
TfiI	GAWTC	5 cut(s)	52, 973, 1009, 1258, 1877
Tru1I	TTAA	4 cut(s)	75, 318, 663, 1901
Tru9I	TTAA	4 cut(s)	75, 318, 663, 1901
TseFI	GTSAC	2 cut(s)	219, 2038
TseI	GCWGC	4 cut(s)	842, 1892, 2078, 2282
Tsp45I	GTSAC	2 cut(s)	219, 2038
TspDTI	ATGAA	9 cut(s)	6, 847, 965, 1017, 1047, 1199, 1386, 1563, 2178
TspGWI	ACGGA	1 cut(s)	1448
Van91I	CCANNNNNTGG	2 cut(s)	37, 449
VpaK11BI	GGWCC	1 cut(s)	4
XapI	RAATTY	3 cut(s)	155, 752, 952
XbaI	TCTAGA	1 cut(s)	2107
XceI	RCATGY	1 cut(s)	1058
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1198
XmiI	GTMKAC	1 cut(s)	276
XmnI	GAANNNNTTC	1 cut(s)	1008
XspI	CTAG	5 cut(s)	869, 1545, 1983, 2028, 2108
Zsp2I	ATGCAT	1 cut(s)	316

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.