Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

21353325 .. 21361520

Gene Structure 8196 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G017360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGAGTAAAATAGCACAACGGTCGTTACAAAACTTAGACTTTAGAGATTTATCCCATGGATTGGGACTTTGCAAGAATCAGGGGTACCAGAAACAGGGAATAGGATGGGACAAATTACATCTATCAAGCACTGCCCTTACATCTCGAGTCTCGTTTCGGAAACTGGATTCTTGGAGTATTGGTTTATCAGAGAATGTATACAGGCCCATACATACTGTACCTCACAGATATCATGTGTTTAAGTGCCATTCATCTGTAACATCAGGCCAATCTTTTGGCCAACATGGCTTGAAGACAGCTACCATGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCGTCATTATATTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGTAACACACACTATGTTACGAACTCATATATTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATTTGCAGAGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTTTGGATTTTATAAGATCACTGTCAAGTGTTCTGTCTTTTGCCTATTGTTTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCTGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTTACTGCAGGAGGTCTTGGGACAGTCTTATTGACTCTGGCTGGTCGTGAGATATTTACAAATTTCCTGTCAAGTGCAATGATTCATGCCACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACGGTTGAGCATGTAGGCTGGTGGTCGCCAACTATTGTAAGAGGTGAAGATCGTGAAGCTGTACACATTCCAAACCATAAGTTCACAGTGAATGTTGTGAGAAATCTCACTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATTAGTCACTTGGATGTCCATAAGATAAATAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCTCAAGTGGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCTGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACGTCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAGCGATACCGATTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAGTATCACCTAGACGCCCGTTGTTGCTAATTGAACCCTCGTACAAGATAAATGGAGAAGATAAAAAAAAAAGTCGGTCAGTGCAGACAAGTGGAGAGCGAGATGGGAAGGCCACAGTGAGGCCACAACCTGACTCCAAGGTCGATGCTAAAGTTGCTGCAACATCAGTATCTGACTCCAAGAAAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACAACCCCACTGCTTCAAAGTCATCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAATACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCGGACCCGATATCTAATCATTTGACGGAGAAGATGTCTGGCAGAAAGCAACCGAATAATGCAAGTGGAAATGTAGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTGCAGAGCCACCAACAAGAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACACTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAGATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

747

Amino Acids

82.45

Weight (kDa)

9.58

Isoelectric Point (pI)

44.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	158 - 287	1.1e-71	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	289 - 359	1.8e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	364 - 450	1.1e-43	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	608
AasI	GACNNNNNNGTC	1 cut(s)	1595
Acc16I	TGCGCA	1 cut(s)	1163
Acc36I	ACCTGC	1 cut(s)	2041
Acc65I	GGTACC	1 cut(s)	84
AccB1I	GGYRCC	1 cut(s)	84
AccB7I	CCANNNNNTGG	2 cut(s)	61, 371
AccI	GTMKAC	1 cut(s)	198
AccIII	TCCGGA	1 cut(s)	2154
AciI	CCGC	3 cut(s)	907, 1433, 1826
AclI	AACGTT	1 cut(s)	1230
AclWI	GGATC	6 cut(s)	542, 555, 652, 1338, 1724, 1972
AcoI	YGGCCR	1 cut(s)	277
AcsI	RAATTY	5 cut(s)	674, 874, 1681, 1783, 2164
AcuI	CTGAAG	1 cut(s)	2157
AcyI	GRCGYC	3 cut(s)	375, 1471, 1676
AfaI	GTAC	6 cut(s)	86, 219, 746, 1029, 1384, 1499
AfiI	CCNNNNNNNGG	7 cut(s)	61, 94, 371, 517, 810, 1357, 1575
AgsI	TTSAA	8 cut(s)	292, 313, 355, 500, 935, 1490, 1686, 1713
AhdI	GACNNNNNGTC	1 cut(s)	1287
AjiI	CACGTC	1 cut(s)	1289
AjnI	CCWGG	2 cut(s)	1670, 2218
AloI	GAACNNNNNNTCC	2 cut(s)	1884, 1916
AluBI	AGCT	8 cut(s)	299, 456, 572, 740, 1025, 1250, 1319, 2213
AluI	AGCT	8 cut(s)	299, 456, 572, 740, 1025, 1250, 1319, 2213
Alw21I	GWGCWC	1 cut(s)	1910
Alw26I	GTCTC	2 cut(s)	154, 404
AlwI	GGATC	6 cut(s)	542, 555, 652, 1338, 1724, 1972
AlwNI	CAGNNNCTG	2 cut(s)	1628, 2180
Ama87I	CYCGRG	1 cut(s)	144
Aor13HI	TCCGGA	1 cut(s)	2154
AoxI	GGCC	6 cut(s)	203, 265, 277, 1359, 1566, 1577
ApeKI	GCWGC	5 cut(s)	453, 764, 1613, 1823, 2213
ApoI	RAATTY	5 cut(s)	674, 874, 1681, 1783, 2164
ArsI	GACNNNNNNTTYG	2 cut(s)	866, 898
Asp700I	GAANNNNTTC	2 cut(s)	930, 2110
Asp718I	GGTACC	1 cut(s)	84
AspLEI	GCGC	1 cut(s)	1164
AspS9I	GGNCC	3 cut(s)	204, 1360, 1828
AsuHPI	GGTGA	4 cut(s)	1022, 1097, 1455, 2006
AvaI	CYCGRG	1 cut(s)	144
AvaII	GGWCC	1 cut(s)	1828
BaeI	ACNNNNGTAYC	2 cut(s)	1608, 1641
BalI	TGGCCA	1 cut(s)	279
BamHI	GGATCC	1 cut(s)	547
BanI	GGYRCC	1 cut(s)	84
BanII	GRGCYC	1 cut(s)	2078
BbsI	GAAGAC	4 cut(s)	299, 1286, 2035, 2163
Bbv12I	GWGCWC	1 cut(s)	1910
BbvI	GCAGC	5 cut(s)	465, 751, 1600, 1810, 2200
BccI	CCATC	4 cut(s)	99, 373, 1359, 1553
BceAI	ACGGC	1 cut(s)	608
BcgI	CGANNNNNNTGC	1 cut(s)	37
BciT130I	CCWGG	2 cut(s)	1672, 2220
BclI	TGATCA	1 cut(s)	2200
BcoDI	GTCTC	2 cut(s)	154, 404
BfaI	CTAG	5 cut(s)	573, 791, 1467, 1914, 2039
BfmI	CTRYAG	2 cut(s)	564, 819
BfuAI	ACCTGC	1 cut(s)	2041
BglI	GCCNNNNNGGC	1 cut(s)	285
BisI	GCNGC	5 cut(s)	454, 765, 1614, 1824, 2214
BlsI	GCNGC	5 cut(s)	455, 766, 1615, 1825, 2215
Bme1390I	CCNGG	2 cut(s)	1672, 2220
Bme18I	GGWCC	1 cut(s)	1828
BmeRI	GACNNNNNGTC	1 cut(s)	1287
BmeT110I	CYCGRG	1 cut(s)	144
BmgBI	CACGTC	1 cut(s)	1289
BmgT120I	GGNCC	3 cut(s)	204, 1360, 1828
BmiI	GGNNCC	4 cut(s)	86, 363, 549, 1830
BmrFI	CCNGG	2 cut(s)	1672, 2220
BmrI	ACTGGG	1 cut(s)	1973
BmsI	GCATC	5 cut(s)	1591, 1657, 1783, 1992, 2122
BmuI	ACTGGG	1 cut(s)	1973
BpiI	GAAGAC	4 cut(s)	299, 1286, 2035, 2163
BplI	GAGNNNNNCTC	2 cut(s)	513, 545
BpuEI	CTTGAG	1 cut(s)	1170
BsaHI	GRCGYC	3 cut(s)	375, 1471, 1676
BsaI	GGTCTC	1 cut(s)	404
BsaJI	CCNNGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
BsaWI	WCCGGW	1 cut(s)	2154
BsaXI	ACNNNNNCTCC	6 cut(s)	166, 196, 492, 522, 1844, 1874
Bsc4I	CCNNNNNNNGG	7 cut(s)	61, 94, 371, 517, 810, 1357, 1575
Bse1I	ACTGG	5 cut(s)	168, 551, 699, 1979, 2006
Bse3DI	GCAATG	2 cut(s)	531, 897
BseAI	TCCGGA	1 cut(s)	2154
BseBI	CCWGG	2 cut(s)	1672, 2220
BseDI	CCNNGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
BseGI	GGATG	3 cut(s)	110, 1132, 1973
BseLI	CCNNNNNNNGG	7 cut(s)	61, 94, 371, 517, 810, 1357, 1575
BseMI	GCAATG	2 cut(s)	531, 897
BseMII	CTCAG	1 cut(s)	410
BseNI	ACTGG	5 cut(s)	168, 551, 699, 1979, 2006
BseXI	GCAGC	5 cut(s)	465, 751, 1600, 1810, 2200
BseYI	CCCAGC	1 cut(s)	731
BsgI	GTGCAG	3 cut(s)	1409, 1559, 2199
Bsh1285I	CGRYCG	1 cut(s)	23
BshFI	GGCC	6 cut(s)	205, 267, 279, 1361, 1568, 1579
BshNI	GGYRCC	1 cut(s)	84
BsiEI	CGRYCG	1 cut(s)	23
BsiHKAI	GWGCWC	1 cut(s)	1910
BsiHKCI	CYCGRG	1 cut(s)	144
BsiSI	CCGG	1 cut(s)	2155
BslFI	GGGAC	3 cut(s)	78, 122, 847
BslI	CCNNNNNNNGG	7 cut(s)	61, 94, 371, 517, 810, 1357, 1575
BsmAI	GTCTC	2 cut(s)	154, 404
BsmFI	GGGAC	3 cut(s)	78, 122, 847
BsnI	GGCC	6 cut(s)	205, 267, 279, 1361, 1568, 1579
Bso31I	GGTCTC	1 cut(s)	404
BsoBI	CYCGRG	1 cut(s)	144
Bsp1286I	GDGCHC	2 cut(s)	1910, 2078
Bsp13I	TCCGGA	1 cut(s)	2154
Bsp1407I	TGTACA	1 cut(s)	1027
Bsp143I	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
Bsp19I	CCATGG	2 cut(s)	55, 303
BspACI	CCGC	3 cut(s)	907, 1433, 1826
BspANI	GGCC	6 cut(s)	205, 267, 279, 1361, 1568, 1579
BspCNI	CTCAG	1 cut(s)	411
BspEI	TCCGGA	1 cut(s)	2154
BspHI	TCATGA	2 cut(s)	1254, 1291
BspLI	GGNNCC	4 cut(s)	86, 363, 549, 1830
BspMAI	CTGCAG	1 cut(s)	823
BspMI	ACCTGC	1 cut(s)	2041
BspPI	GGATC	6 cut(s)	542, 555, 652, 1338, 1724, 1972
BspT107I	GGYRCC	1 cut(s)	84
BspTNI	GGTCTC	1 cut(s)	404
BsrDI	GCAATG	2 cut(s)	531, 897
BsrGI	TGTACA	1 cut(s)	1027
BsrI	ACTGG	5 cut(s)	168, 551, 699, 1979, 2006
BssECI	CCNNGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
BssMI	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
BssNAI	GTATAC	1 cut(s)	199
BssNI	GRCGYC	3 cut(s)	375, 1471, 1676
BssT1I	CCWWGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
Bst1107I	GTATAC	1 cut(s)	199
Bst2UI	CCWGG	2 cut(s)	1672, 2220
Bst4CI	ACNGT	9 cut(s)	21, 217, 618, 838, 970, 1054, 1387, 1421, 1573
Bst6I	CTCTTC	2 cut(s)	1207, 1290
BstACI	GRCGYC	3 cut(s)	375, 1471, 1676
BstAPI	GCANNNNNTGC	2 cut(s)	1204, 1946
BstAUI	TGTACA	1 cut(s)	1027
BstDEI	CTNAG	4 cut(s)	34, 419, 437, 1337
BstDSI	CCRYGG	2 cut(s)	55, 303
BstF5I	GGATG	3 cut(s)	110, 1132, 1973
BstHHI	GCGC	1 cut(s)	1164
BstKTI	GATC	9 cut(s)	550, 614, 660, 1018, 1260, 1333, 1732, 1967, 2203
BstMAI	GTCTC	2 cut(s)	154, 404
BstMBI	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
BstMCI	CGRYCG	1 cut(s)	23
BstMWI	GCNNNNNNNGC	6 cut(s)	285, 737, 1204, 1367, 1610, 1946
BstNI	CCWGG	2 cut(s)	1672, 2220
BstNSI	RCATGY	2 cut(s)	980, 1162
BstSCI	CCNGG	2 cut(s)	1670, 2218
BstSFI	CTRYAG	2 cut(s)	564, 819
BstV1I	GCAGC	5 cut(s)	465, 751, 1600, 1810, 2200
BstV2I	GAAGAC	4 cut(s)	299, 1286, 2035, 2163
BstX2I	RGATCY	2 cut(s)	547, 1330
BstXI	CCANNNNNNTGG	2 cut(s)	275, 731
BstYI	RGATCY	2 cut(s)	547, 1330
BstZ17I	GTATAC	1 cut(s)	199
BsuRI	GGCC	6 cut(s)	205, 267, 279, 1361, 1568, 1579
BtgI	CCRYGG	2 cut(s)	55, 303
BtrI	CACGTC	1 cut(s)	1289
BtsCI	GGATG	3 cut(s)	110, 1132, 1973
BtsI	GCAGTG	2 cut(s)	129, 1704
BveI	ACCTGC	1 cut(s)	2041
CaiI	CAGNNNCTG	2 cut(s)	1628, 2180
CciI	TCATGA	2 cut(s)	1254, 1291
CfoI	GCGC	1 cut(s)	1164
Cfr13I	GGNCC	3 cut(s)	204, 1360, 1828
CseI	GACGC	4 cut(s)	364, 404, 1479, 1684
Csp6I	GTAC	6 cut(s)	85, 218, 745, 1028, 1383, 1498
CspCI	CAANNNNNGTGG	2 cut(s)	1942, 1977
CviQI	GTAC	6 cut(s)	85, 218, 745, 1028, 1383, 1498
DdeI	CTNAG	4 cut(s)	34, 419, 437, 1337
DpnI	GATC	9 cut(s)	549, 613, 659, 1017, 1259, 1332, 1731, 1966, 2202
DpnII	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
DrdI	GACNNNNNNGTC	1 cut(s)	1595
DriI	GACNNNNNGTC	1 cut(s)	1287
DseDI	GACNNNNNNGTC	1 cut(s)	1595
EaeI	YGGCCR	1 cut(s)	277
Eam1104I	CTCTTC	2 cut(s)	1207, 1290
Eam1105I	GACNNNNNGTC	1 cut(s)	1287
EarI	CTCTTC	2 cut(s)	1207, 1290
Eco130I	CCWWGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
Eco24I	GRGCYC	1 cut(s)	2078
Eco31I	GGTCTC	1 cut(s)	404
Eco32I	GATATC	2 cut(s)	230, 1836
Eco47I	GGWCC	1 cut(s)	1828
Eco57I	CTGAAG	1 cut(s)	2157
Eco88I	CYCGRG	1 cut(s)	144
EcoRII	CCWGG	2 cut(s)	1670, 2218
EcoRV	GATATC	2 cut(s)	230, 1836
EcoT14I	CCWWGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
EcoT38I	GRGCYC	1 cut(s)	2078
ErhI	CCWWGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
FaqI	GGGAC	3 cut(s)	78, 122, 847
FauI	CCCGC	1 cut(s)	914
FauNDI	CATATG	2 cut(s)	322, 392
FbaI	TGATCA	1 cut(s)	2200
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	5 cut(s)	454, 765, 1614, 1824, 2214
FokI	GGATG	3 cut(s)	117, 1139, 1960
FriOI	GRGCYC	1 cut(s)	2078
Fsp4HI	GCNGC	5 cut(s)	454, 765, 1614, 1824, 2214
FspBI	CTAG	5 cut(s)	573, 791, 1467, 1914, 2039
FspI	TGCGCA	1 cut(s)	1163
GlaI	GCGC	1 cut(s)	1163
GluI	GCNGC	5 cut(s)	454, 765, 1614, 1824, 2214
GsaI	CCCAGC	1 cut(s)	735
HaeIII	GGCC	6 cut(s)	205, 267, 279, 1361, 1568, 1579
HapII	CCGG	1 cut(s)	2155
HgaI	GACGC	4 cut(s)	364, 404, 1479, 1684
HhaI	GCGC	1 cut(s)	1164
Hin1I	GRCGYC	3 cut(s)	375, 1471, 1676
Hin6I	GCGC	1 cut(s)	1162
HinP1I	GCGC	1 cut(s)	1162
HpaII	CCGG	1 cut(s)	2155
HphI	GGTGA	4 cut(s)	1022, 1097, 1455, 2006
Hpy166II	GTNNAC	4 cut(s)	199, 745, 1030, 1050
Hpy8I	GTNNAC	4 cut(s)	199, 745, 1030, 1050
HpyAV	CCTTC	8 cut(s)	941, 1558, 1734, 1985, 2096, 2099, 2127, 2157
HpyCH4III	ACNGT	9 cut(s)	21, 217, 618, 838, 970, 1054, 1387, 1421, 1573
HpyCH4IV	ACGT	2 cut(s)	1230, 1288
HpyF10VI	GCNNNNNNNGC	6 cut(s)	285, 737, 1204, 1367, 1610, 1946
HpyF3I	CTNAG	4 cut(s)	34, 419, 437, 1337
HpySE526I	ACGT	2 cut(s)	1230, 1288
Hsp92I	GRCGYC	3 cut(s)	375, 1471, 1676
HspAI	GCGC	1 cut(s)	1162
Kpn2I	TCCGGA	1 cut(s)	2154
KpnI	GGTACC	1 cut(s)	88
Ksp22I	TGATCA	1 cut(s)	2200
Kzo9I	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
LmnI	GCTCC	2 cut(s)	513, 521
Lsp1109I	GCAGC	5 cut(s)	465, 751, 1600, 1810, 2200
LweI	GCATC	5 cut(s)	1591, 1657, 1783, 1992, 2122
MaeI	CTAG	5 cut(s)	573, 791, 1467, 1914, 2039
MaeII	ACGT	2 cut(s)	1230, 1288
MaeIII	GTNAC	7 cut(s)	24, 256, 467, 481, 689, 1118, 1231
MalI	GATC	9 cut(s)	549, 613, 659, 1017, 1259, 1332, 1731, 1966, 2202
MboI	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
MflI	RGATCY	2 cut(s)	547, 1330
MhlI	GDGCHC	2 cut(s)	1910, 2078
MlsI	TGGCCA	1 cut(s)	279
MluCI	AATT	9 cut(s)	113, 356, 674, 725, 874, 1485, 1681, 1783, 2164
MluNI	TGGCCA	1 cut(s)	279
MlyI	GAGTC	8 cut(s)	156, 704, 841, 1350, 1430, 1583, 1625, 1808
MmeI	TCCRAC	1 cut(s)	685
Mox20I	TGGCCA	1 cut(s)	279
MroI	TCCGGA	1 cut(s)	2154
MroXI	GAANNNNTTC	2 cut(s)	930, 2110
MscI	TGGCCA	1 cut(s)	279
MseI	TTAA	2 cut(s)	240, 585
MslI	CAYNNNNRTG	2 cut(s)	1125, 2130
Msp20I	TGGCCA	1 cut(s)	279
MspA1I	CMGCKG	2 cut(s)	456, 2213
MspI	CCGG	1 cut(s)	2155
MspR9I	CCNGG	2 cut(s)	1672, 2220
MvaI	CCWGG	2 cut(s)	1672, 2220
MwoI	GCNNNNNNNGC	6 cut(s)	285, 737, 1204, 1367, 1610, 1946
NcoI	CCATGG	2 cut(s)	55, 303
NdeI	CATATG	2 cut(s)	322, 392
NdeII	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
NlaIV	GGNNCC	4 cut(s)	86, 363, 549, 1830
NmuCI	GTSAC	1 cut(s)	1118
NsbI	TGCGCA	1 cut(s)	1163
NspI	RCATGY	2 cut(s)	980, 1162
PaeR7I	CTCGAG	1 cut(s)	144
PagI	TCATGA	2 cut(s)	1254, 1291
PdmI	GAANNNNTTC	2 cut(s)	930, 2110
PfeI	GAWTC	6 cut(s)	76, 167, 895, 931, 1180, 1808
PflMI	CCANNNNNTGG	2 cut(s)	61, 371
PkrI	GCNGC	5 cut(s)	455, 766, 1615, 1825, 2215
PleI	GAGTC	8 cut(s)	155, 703, 841, 1349, 1430, 1583, 1625, 1808
PpsI	GAGTC	8 cut(s)	155, 703, 841, 1349, 1430, 1583, 1625, 1808
PsiI	TTATAA	1 cut(s)	608
Psp1406I	AACGTT	1 cut(s)	1230
Psp6I	CCWGG	2 cut(s)	1670, 2218
PspFI	CCCAGC	1 cut(s)	731
PspGI	CCWGG	2 cut(s)	1670, 2218
PspN4I	GGNNCC	4 cut(s)	86, 363, 549, 1830
PspPI	GGNCC	3 cut(s)	204, 1360, 1828
PstI	CTGCAG	1 cut(s)	823
PstNI	CAGNNNCTG	2 cut(s)	1628, 2180
PsuI	RGATCY	2 cut(s)	547, 1330
PvuII	CAGCTG	2 cut(s)	456, 2213
RsaI	GTAC	6 cut(s)	86, 219, 746, 1029, 1384, 1499
RsaNI	GTAC	6 cut(s)	85, 218, 745, 1028, 1383, 1498
RseI	CAYNNNNRTG	2 cut(s)	1125, 2130
SaqAI	TTAA	2 cut(s)	240, 585
SatI	GCNGC	5 cut(s)	454, 765, 1614, 1824, 2214
Sau3AI	GATC	9 cut(s)	547, 611, 657, 1015, 1257, 1330, 1729, 1964, 2200
Sau96I	GGNCC	3 cut(s)	204, 1360, 1828
SchI	GAGTC	8 cut(s)	156, 704, 841, 1350, 1430, 1583, 1625, 1808
ScrFI	CCNGG	2 cut(s)	1672, 2220
SduI	GDGCHC	2 cut(s)	1910, 2078
SfaNI	GCATC	5 cut(s)	1591, 1657, 1783, 1992, 2122
SfcI	CTRYAG	2 cut(s)	564, 819
Sfr274I	CTCGAG	1 cut(s)	144
SinI	GGWCC	1 cut(s)	1828
SlaI	CTCGAG	1 cut(s)	144
SmiMI	CAYNNNNRTG	2 cut(s)	1125, 2130
SmlI	CTYRAG	2 cut(s)	144, 1185
SmoI	CTYRAG	2 cut(s)	144, 1185
Sse9I	AATT	9 cut(s)	113, 356, 674, 725, 874, 1485, 1681, 1783, 2164
SsiI	CCGC	3 cut(s)	907, 1433, 1826
SspMI	CTAG	5 cut(s)	573, 791, 1467, 1914, 2039
StyD4I	CCNGG	2 cut(s)	1670, 2218
StyI	CCWWGG	8 cut(s)	55, 303, 1593, 1659, 1692, 1818, 2056, 2067
TaaI	ACNGT	9 cut(s)	21, 217, 618, 838, 970, 1054, 1387, 1421, 1573
TaiI	ACGT	2 cut(s)	1233, 1291
TaqI	TCGA	3 cut(s)	145, 1599, 1769
TaqII	GACCGA	1 cut(s)	1521
TasI	AATT	9 cut(s)	113, 356, 674, 725, 874, 1485, 1681, 1783, 2164
TatI	WGTACW	2 cut(s)	744, 1027
TfiI	GAWTC	6 cut(s)	76, 167, 895, 931, 1180, 1808
Tru1I	TTAA	2 cut(s)	240, 585
Tru9I	TTAA	2 cut(s)	240, 585
TseFI	GTSAC	1 cut(s)	1118
TseI	GCWGC	5 cut(s)	453, 764, 1613, 1823, 2213
Tsp45I	GTSAC	1 cut(s)	1118
TspDTI	ATGAA	7 cut(s)	240, 769, 887, 939, 969, 1308, 2109
TspGWI	ACGGA	2 cut(s)	1370, 1865
Van91I	CCANNNNNTGG	2 cut(s)	61, 371
VpaK11BI	GGWCC	1 cut(s)	1828
XapI	RAATTY	5 cut(s)	674, 874, 1681, 1783, 2164
XbaI	TCTAGA	1 cut(s)	2038
XceI	RCATGY	2 cut(s)	980, 1162
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1120
XhoI	CTCGAG	1 cut(s)	144
XmiI	GTMKAC	1 cut(s)	198
XmnI	GAANNNNTTC	2 cut(s)	930, 2110
XspI	CTAG	5 cut(s)	573, 791, 1467, 1914, 2039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.