Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

23043737 .. 23051212

Gene Structure 7476 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG232400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCATCATTATATTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGTAACACACACCATGTTACGAACTCATATATTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATTTGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTTTGGATTTTATAAGATCGCTGTCAACTGTTCTGTCTTTTGCCTATTGTTTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCTGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTTACTGCAGGAGGTCTTGGGACAGTCTTATTGACTCTGGCTGGTCGTGAGATATTTACAAATTTCCTGTCAAGTGCAATGATTCATGCCACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACAGTTGAGCATGTAGGTTGGTGGTCGCCAACTATTGTAAGAGGTGAAGATCGTGAAGCTGTACATATCCCAAACCATAAGTTCACAGTGAATGTTGTGAGAAATCTCACTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATTAGTCACTTGGATGTCCATAAGATAAATAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCTCAAGTGGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCTGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACGTCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAGCGATACCGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAGTATCACCTAGACGCCCGTTGTTGCTAATTGAACCCTCGTACAAGATAAATGGAGAAGATAAAAAAAAAAGTCGTTCAGTGCAGACCAGTGGAGAGCGAGATGGGAAGGCCACAGTGCGGCCACAACCTGACTCCAAGGTCGATGCTAAAGTTGCTGCAACATCAGTATCTGACTCCAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACAACCCCACTGCTTCAAAGTCATCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAATACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCGGACCCGATATCTAATCATTTGACGGAGAAGATGTCTGGCAGAAAGCAACTGAATAATGCAAGTGGAAATGTAGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTGCAGAGCCACCAACAAGAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACACTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAAATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

646

Amino Acids

71.09

Weight (kDa)

9.47

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	57 - 186	3.8e-72	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	188 - 258	1.5e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	263 - 349	8.8e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	305
AasI	GACNNNNNNGTC	1 cut(s)	1292
Acc16I	TGCGCA	1 cut(s)	860
Acc36I	ACCTGC	1 cut(s)	1738
AccB7I	CCANNNNNTGG	1 cut(s)	68
AccIII	TCCGGA	1 cut(s)	1851
AciI	CCGC	4 cut(s)	604, 1130, 1273, 1523
AclI	AACGTT	1 cut(s)	927
AclWI	GGATC	6 cut(s)	239, 252, 349, 1035, 1421, 1669
AcoI	YGGCCR	1 cut(s)	1274
AcsI	RAATTY	5 cut(s)	371, 571, 1378, 1480, 1861
AcyI	GRCGYC	2 cut(s)	1168, 1373
AfaI	GTAC	4 cut(s)	443, 726, 1081, 1196
AfiI	CCNNNNNNNGG	5 cut(s)	68, 214, 507, 1054, 1272
AgsI	TTSAA	8 cut(s)	10, 52, 197, 632, 1187, 1383, 1410, 1872
AhdI	GACNNNNNGTC	1 cut(s)	984
AjiI	CACGTC	1 cut(s)	986
AjnI	CCWGG	2 cut(s)	1367, 1915
AloI	GAACNNNNNNTCC	2 cut(s)	1581, 1613
AluBI	AGCT	7 cut(s)	153, 269, 437, 722, 947, 1016, 1910
AluI	AGCT	7 cut(s)	153, 269, 437, 722, 947, 1016, 1910
Alw21I	GWGCWC	1 cut(s)	1607
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	6 cut(s)	239, 252, 349, 1035, 1421, 1669
AlwNI	CAGNNNCTG	1 cut(s)	1325
Aor13HI	TCCGGA	1 cut(s)	1851
AoxI	GGCC	3 cut(s)	1056, 1263, 1274
ApeKI	GCWGC	5 cut(s)	150, 461, 1310, 1520, 1910
ApoI	RAATTY	5 cut(s)	371, 571, 1378, 1480, 1861
ArsI	GACNNNNNNTTYG	2 cut(s)	563, 595
Asp700I	GAANNNNTTC	2 cut(s)	627, 1807
AspLEI	GCGC	1 cut(s)	861
AspS9I	GGNCC	2 cut(s)	1057, 1525
AsuHPI	GGTGA	4 cut(s)	719, 794, 1152, 1703
AvaII	GGWCC	1 cut(s)	1525
BaeI	ACNNNNGTAYC	2 cut(s)	1305, 1338
BamHI	GGATCC	1 cut(s)	244
BanII	GRGCYC	1 cut(s)	1775
BbsI	GAAGAC	3 cut(s)	983, 1732, 1860
Bbv12I	GWGCWC	1 cut(s)	1607
BbvI	GCAGC	5 cut(s)	162, 448, 1297, 1507, 1897
BccI	CCATC	3 cut(s)	70, 1056, 1250
BceAI	ACGGC	1 cut(s)	305
BciT130I	CCWGG	2 cut(s)	1369, 1917
BclI	TGATCA	1 cut(s)	1897
BcoDI	GTCTC	1 cut(s)	101
BfaI	CTAG	5 cut(s)	270, 488, 1164, 1611, 1736
BfmI	CTRYAG	2 cut(s)	261, 516
BfuAI	ACCTGC	1 cut(s)	1738
BisI	GCNGC	6 cut(s)	151, 462, 1274, 1311, 1521, 1911
BlsI	GCNGC	6 cut(s)	152, 463, 1275, 1312, 1522, 1912
Bme1390I	CCNGG	2 cut(s)	1369, 1917
Bme18I	GGWCC	1 cut(s)	1525
BmeRI	GACNNNNNGTC	1 cut(s)	984
BmgBI	CACGTC	1 cut(s)	986
BmgT120I	GGNCC	2 cut(s)	1057, 1525
BmiI	GGNNCC	3 cut(s)	60, 246, 1527
BmrFI	CCNGG	2 cut(s)	1369, 1917
BmrI	ACTGGG	1 cut(s)	1670
BmsI	GCATC	6 cut(s)	81, 1288, 1354, 1480, 1689, 1819
BmuI	ACTGGG	1 cut(s)	1670
BpiI	GAAGAC	3 cut(s)	983, 1732, 1860
BplI	GAGNNNNNCTC	2 cut(s)	210, 242
BpuEI	CTTGAG	1 cut(s)	867
BsaHI	GRCGYC	2 cut(s)	1168, 1373
BsaI	GGTCTC	1 cut(s)	101
BsaJI	CCNNGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
BsaWI	WCCGGW	1 cut(s)	1851
BsaXI	ACNNNNNCTCC	4 cut(s)	189, 219, 1541, 1571
Bsc4I	CCNNNNNNNGG	5 cut(s)	68, 214, 507, 1054, 1272
Bse1I	ACTGG	5 cut(s)	248, 396, 1242, 1676, 1703
Bse3DI	GCAATG	2 cut(s)	228, 594
BseAI	TCCGGA	1 cut(s)	1851
BseBI	CCWGG	2 cut(s)	1369, 1917
BseDI	CCNNGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
BseGI	GGATG	2 cut(s)	829, 1670
BseLI	CCNNNNNNNGG	5 cut(s)	68, 214, 507, 1054, 1272
BseMI	GCAATG	2 cut(s)	228, 594
BseMII	CTCAG	1 cut(s)	107
BseNI	ACTGG	5 cut(s)	248, 396, 1242, 1676, 1703
BseXI	GCAGC	5 cut(s)	162, 448, 1297, 1507, 1897
BseYI	CCCAGC	1 cut(s)	428
BsgI	GTGCAG	3 cut(s)	1106, 1256, 1896
BshFI	GGCC	3 cut(s)	1058, 1265, 1276
BsiHKAI	GWGCWC	1 cut(s)	1607
BsiSI	CCGG	1 cut(s)	1852
BslFI	GGGAC	1 cut(s)	544
BslI	CCNNNNNNNGG	5 cut(s)	68, 214, 507, 1054, 1272
BsmAI	GTCTC	1 cut(s)	101
BsmFI	GGGAC	1 cut(s)	544
BsnI	GGCC	3 cut(s)	1058, 1265, 1276
Bso31I	GGTCTC	1 cut(s)	101
Bsp1286I	GDGCHC	2 cut(s)	1607, 1775
Bsp13I	TCCGGA	1 cut(s)	1851
Bsp1407I	TGTACA	1 cut(s)	724
Bsp143I	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
BspACI	CCGC	4 cut(s)	604, 1130, 1273, 1523
BspANI	GGCC	3 cut(s)	1058, 1265, 1276
BspCNI	CTCAG	1 cut(s)	108
BspEI	TCCGGA	1 cut(s)	1851
BspHI	TCATGA	2 cut(s)	951, 988
BspLI	GGNNCC	3 cut(s)	60, 246, 1527
BspMAI	CTGCAG	1 cut(s)	520
BspMI	ACCTGC	1 cut(s)	1738
BspPI	GGATC	6 cut(s)	239, 252, 349, 1035, 1421, 1669
BspTNI	GGTCTC	1 cut(s)	101
BsrDI	GCAATG	2 cut(s)	228, 594
BsrGI	TGTACA	1 cut(s)	724
BsrI	ACTGG	5 cut(s)	248, 396, 1242, 1676, 1703
BssECI	CCNNGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
BssMI	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
BssNI	GRCGYC	2 cut(s)	1168, 1373
BssT1I	CCWWGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
Bst2UI	CCWGG	2 cut(s)	1369, 1917
Bst4CI	ACNGT	7 cut(s)	322, 535, 667, 751, 1084, 1118, 1270
Bst6I	CTCTTC	2 cut(s)	904, 987
BstACI	GRCGYC	2 cut(s)	1168, 1373
BstAPI	GCANNNNNTGC	2 cut(s)	901, 1643
BstAUI	TGTACA	1 cut(s)	724
BstDEI	CTNAG	3 cut(s)	116, 134, 1034
BstF5I	GGATG	2 cut(s)	829, 1670
BstHHI	GCGC	1 cut(s)	861
BstKTI	GATC	9 cut(s)	247, 311, 357, 715, 957, 1030, 1429, 1664, 1900
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
BstMWI	GCNNNNNNNGC	5 cut(s)	434, 901, 1064, 1307, 1643
BstNI	CCWGG	2 cut(s)	1369, 1917
BstNSI	RCATGY	2 cut(s)	677, 859
BstSCI	CCNGG	2 cut(s)	1367, 1915
BstSFI	CTRYAG	2 cut(s)	261, 516
BstV1I	GCAGC	5 cut(s)	162, 448, 1297, 1507, 1897
BstV2I	GAAGAC	3 cut(s)	983, 1732, 1860
BstX2I	RGATCY	2 cut(s)	244, 1027
BstXI	CCANNNNNNTGG	1 cut(s)	428
BstYI	RGATCY	2 cut(s)	244, 1027
BsuRI	GGCC	3 cut(s)	1058, 1265, 1276
BtrI	CACGTC	1 cut(s)	986
BtsCI	GGATG	2 cut(s)	829, 1670
BtsI	GCAGTG	1 cut(s)	1401
BtsIMutI	CAGTG	9 cut(s)	403, 756, 1089, 1239, 1249, 1275, 1401, 1683, 1824
BveI	ACCTGC	1 cut(s)	1738
CaiI	CAGNNNCTG	1 cut(s)	1325
CciI	TCATGA	2 cut(s)	951, 988
CfoI	GCGC	1 cut(s)	861
Cfr13I	GGNCC	2 cut(s)	1057, 1525
CseI	GACGC	3 cut(s)	101, 1176, 1381
Csp6I	GTAC	4 cut(s)	442, 725, 1080, 1195
CspCI	CAANNNNNGTGG	2 cut(s)	1639, 1674
CviAII	CATG	8 cut(s)	176, 478, 596, 674, 856, 952, 989, 1024
CviQI	GTAC	4 cut(s)	442, 725, 1080, 1195
DdeI	CTNAG	3 cut(s)	116, 134, 1034
DpnI	GATC	9 cut(s)	246, 310, 356, 714, 956, 1029, 1428, 1663, 1899
DpnII	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
DrdI	GACNNNNNNGTC	1 cut(s)	1292
DriI	GACNNNNNGTC	1 cut(s)	984
DseDI	GACNNNNNNGTC	1 cut(s)	1292
EaeI	YGGCCR	1 cut(s)	1274
Eam1104I	CTCTTC	2 cut(s)	904, 987
Eam1105I	GACNNNNNGTC	1 cut(s)	984
EarI	CTCTTC	2 cut(s)	904, 987
Eco130I	CCWWGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
Eco24I	GRGCYC	1 cut(s)	1775
Eco31I	GGTCTC	1 cut(s)	101
Eco32I	GATATC	1 cut(s)	1533
Eco47I	GGWCC	1 cut(s)	1525
EcoRII	CCWGG	2 cut(s)	1367, 1915
EcoRV	GATATC	1 cut(s)	1533
EcoT14I	CCWWGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
EcoT38I	GRGCYC	1 cut(s)	1775
ErhI	CCWWGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
FaeI	CATG	8 cut(s)	179, 481, 599, 677, 859, 955, 992, 1027
FaqI	GGGAC	1 cut(s)	544
FatI	CATG	8 cut(s)	175, 477, 595, 673, 855, 951, 988, 1023
FauI	CCCGC	1 cut(s)	611
FauNDI	CATATG	2 cut(s)	19, 89
FbaI	TGATCA	1 cut(s)	1897
Fnu4HI	GCNGC	6 cut(s)	151, 462, 1274, 1311, 1521, 1911
FokI	GGATG	2 cut(s)	836, 1657
FriOI	GRGCYC	1 cut(s)	1775
Fsp4HI	GCNGC	6 cut(s)	151, 462, 1274, 1311, 1521, 1911
FspBI	CTAG	5 cut(s)	270, 488, 1164, 1611, 1736
FspI	TGCGCA	1 cut(s)	860
GlaI	GCGC	1 cut(s)	860
GluI	GCNGC	6 cut(s)	151, 462, 1274, 1311, 1521, 1911
GsaI	CCCAGC	1 cut(s)	432
HaeIII	GGCC	3 cut(s)	1058, 1265, 1276
HapII	CCGG	1 cut(s)	1852
HgaI	GACGC	3 cut(s)	101, 1176, 1381
HhaI	GCGC	1 cut(s)	861
Hin1I	GRCGYC	2 cut(s)	1168, 1373
Hin1II	CATG	8 cut(s)	179, 481, 599, 677, 859, 955, 992, 1027
Hin6I	GCGC	1 cut(s)	859
HinP1I	GCGC	1 cut(s)	859
HincII	GTYRAC	1 cut(s)	318
HindII	GTYRAC	1 cut(s)	318
HpaII	CCGG	1 cut(s)	1852
HphI	GGTGA	4 cut(s)	719, 794, 1152, 1703
Hpy166II	GTNNAC	3 cut(s)	318, 442, 747
Hpy188I	TCNGA	8 cut(s)	117, 1327, 1351, 1426, 1504, 1510, 1602, 1930
Hpy188III	TCNNGA	9 cut(s)	557, 716, 920, 935, 952, 958, 989, 1736, 1852
Hpy8I	GTNNAC	3 cut(s)	318, 442, 747
HpyAV	CCTTC	8 cut(s)	638, 1255, 1431, 1682, 1793, 1796, 1824, 1854
HpyCH4III	ACNGT	7 cut(s)	322, 535, 667, 751, 1084, 1118, 1270
HpyCH4IV	ACGT	2 cut(s)	927, 985
HpyF10VI	GCNNNNNNNGC	5 cut(s)	434, 901, 1064, 1307, 1643
HpyF3I	CTNAG	3 cut(s)	116, 134, 1034
HpySE526I	ACGT	2 cut(s)	927, 985
Hsp92I	GRCGYC	2 cut(s)	1168, 1373
Hsp92II	CATG	8 cut(s)	179, 481, 599, 677, 859, 955, 992, 1027
HspAI	GCGC	1 cut(s)	859
Kpn2I	TCCGGA	1 cut(s)	1851
Ksp22I	TGATCA	1 cut(s)	1897
Kzo9I	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
LmnI	GCTCC	2 cut(s)	210, 218
Lsp1109I	GCAGC	5 cut(s)	162, 448, 1297, 1507, 1897
LweI	GCATC	6 cut(s)	81, 1288, 1354, 1480, 1689, 1819
MaeI	CTAG	5 cut(s)	270, 488, 1164, 1611, 1736
MaeII	ACGT	2 cut(s)	927, 985
MaeIII	GTNAC	5 cut(s)	164, 178, 386, 815, 928
MalI	GATC	9 cut(s)	246, 310, 356, 714, 956, 1029, 1428, 1663, 1899
MboI	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
MflI	RGATCY	2 cut(s)	244, 1027
MhlI	GDGCHC	2 cut(s)	1607, 1775
MluCI	AATT	8 cut(s)	53, 371, 422, 571, 1182, 1378, 1480, 1861
MlyI	GAGTC	7 cut(s)	401, 538, 1047, 1127, 1280, 1322, 1505
MmeI	TCCRAC	1 cut(s)	382
MnlI	CCTC	9 cut(s)	51, 515, 698, 891, 891, 1201, 1639, 1762, 1789
MroI	TCCGGA	1 cut(s)	1851
MroXI	GAANNNNTTC	2 cut(s)	627, 1807
MseI	TTAA	1 cut(s)	282
MslI	CAYNNNNRTG	2 cut(s)	822, 1827
MspA1I	CMGCKG	2 cut(s)	153, 1910
MspI	CCGG	1 cut(s)	1852
MspR9I	CCNGG	2 cut(s)	1369, 1917
MvaI	CCWGG	2 cut(s)	1369, 1917
MwoI	GCNNNNNNNGC	5 cut(s)	434, 901, 1064, 1307, 1643
NdeI	CATATG	2 cut(s)	19, 89
NdeII	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
NlaIII	CATG	8 cut(s)	179, 481, 599, 677, 859, 955, 992, 1027
NlaIV	GGNNCC	3 cut(s)	60, 246, 1527
NmuCI	GTSAC	1 cut(s)	815
NsbI	TGCGCA	1 cut(s)	860
NspI	RCATGY	2 cut(s)	677, 859
PagI	TCATGA	2 cut(s)	951, 988
PdmI	GAANNNNTTC	2 cut(s)	627, 1807
PfeI	GAWTC	4 cut(s)	592, 628, 877, 1505
PflMI	CCANNNNNTGG	1 cut(s)	68
PkrI	GCNGC	6 cut(s)	152, 463, 1275, 1312, 1522, 1912
PleI	GAGTC	7 cut(s)	400, 538, 1046, 1127, 1280, 1322, 1505
PpsI	GAGTC	7 cut(s)	400, 538, 1046, 1127, 1280, 1322, 1505
PsiI	TTATAA	1 cut(s)	305
Psp1406I	AACGTT	1 cut(s)	927
Psp6I	CCWGG	2 cut(s)	1367, 1915
PspFI	CCCAGC	1 cut(s)	428
PspGI	CCWGG	2 cut(s)	1367, 1915
PspN4I	GGNNCC	3 cut(s)	60, 246, 1527
PspPI	GGNCC	2 cut(s)	1057, 1525
PstI	CTGCAG	1 cut(s)	520
PstNI	CAGNNNCTG	1 cut(s)	1325
PsuI	RGATCY	2 cut(s)	244, 1027
PvuII	CAGCTG	2 cut(s)	153, 1910
RsaI	GTAC	4 cut(s)	443, 726, 1081, 1196
RsaNI	GTAC	4 cut(s)	442, 725, 1080, 1195
RseI	CAYNNNNRTG	2 cut(s)	822, 1827
SaqAI	TTAA	1 cut(s)	282
SatI	GCNGC	6 cut(s)	151, 462, 1274, 1311, 1521, 1911
Sau3AI	GATC	9 cut(s)	244, 308, 354, 712, 954, 1027, 1426, 1661, 1897
Sau96I	GGNCC	2 cut(s)	1057, 1525
SchI	GAGTC	7 cut(s)	401, 538, 1047, 1127, 1280, 1322, 1505
ScrFI	CCNGG	2 cut(s)	1369, 1917
SduI	GDGCHC	2 cut(s)	1607, 1775
SfaNI	GCATC	6 cut(s)	81, 1288, 1354, 1480, 1689, 1819
SfcI	CTRYAG	2 cut(s)	261, 516
SinI	GGWCC	1 cut(s)	1525
SmiMI	CAYNNNNRTG	2 cut(s)	822, 1827
SmlI	CTYRAG	1 cut(s)	882
SmoI	CTYRAG	1 cut(s)	882
Sse9I	AATT	8 cut(s)	53, 371, 422, 571, 1182, 1378, 1480, 1861
SsiI	CCGC	4 cut(s)	604, 1130, 1273, 1523
SspMI	CTAG	5 cut(s)	270, 488, 1164, 1611, 1736
StyD4I	CCNGG	2 cut(s)	1367, 1915
StyI	CCWWGG	6 cut(s)	1290, 1356, 1389, 1515, 1753, 1764
TaaI	ACNGT	7 cut(s)	322, 535, 667, 751, 1084, 1118, 1270
TaiI	ACGT	2 cut(s)	930, 988
TaqI	TCGA	2 cut(s)	1296, 1466
TasI	AATT	8 cut(s)	53, 371, 422, 571, 1182, 1378, 1480, 1861
TatI	WGTACW	2 cut(s)	441, 724
TauI	GCSGC	1 cut(s)	1276
TfiI	GAWTC	4 cut(s)	592, 628, 877, 1505
Tru1I	TTAA	1 cut(s)	282
Tru9I	TTAA	1 cut(s)	282
TscAI	CASTG	9 cut(s)	403, 756, 1089, 1239, 1249, 1275, 1408, 1683, 1831
TseFI	GTSAC	1 cut(s)	815
TseI	GCWGC	5 cut(s)	150, 461, 1310, 1520, 1910
Tsp45I	GTSAC	1 cut(s)	815
TspDTI	ATGAA	6 cut(s)	466, 584, 636, 666, 1005, 1806
TspGWI	ACGGA	2 cut(s)	1067, 1562
TspRI	CASTG	9 cut(s)	403, 756, 1089, 1239, 1249, 1275, 1408, 1683, 1831
Van91I	CCANNNNNTGG	1 cut(s)	68
VpaK11BI	GGWCC	1 cut(s)	1525
XapI	RAATTY	5 cut(s)	371, 571, 1378, 1480, 1861
XbaI	TCTAGA	1 cut(s)	1735
XceI	RCATGY	2 cut(s)	677, 859
XcmI	CCANNNNNNNNNTGG	1 cut(s)	817
XmnI	GAANNNNTTC	2 cut(s)	627, 1807
XspI	CTAG	5 cut(s)	270, 488, 1164, 1611, 1736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.