Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

22520281 .. 22526718

Gene Structure 6438 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017875

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGGTCCTCACTGGTTCGTTGCAATTATCCCATGGATGGGGACTTTGCAAGAATCAGGGGTACCAGAAACAGTTTAAGGGGGGAATAGGAAGGGACAAATTACATCTATCAAGCACTGCCCTTACATCACGAGTCTCGTTTCAGAATCTAGATTCTTGGAGTATTGGGTTATCAGAGAATGTATACAGGCCCATACATACTGTTTCTCAAAGATATCATGCGTTTAAGTGCCATTCTTCTGTAACATCAGGCCAACCATTTGGCCAACATTGCTTGAAGACAGCTACCACGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCGTCATTATCTTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGCAACACACACTATGTTACAAACTCGTATATTCAACCTTTGCTCCTATGGACAGGAGCAATGCTCATTTGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTCTGGACTTTATAAGATCGCTCTCAACTGTTCTGTCTTTTGCCTATTGTTTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATGCACACATATATCATCAATATGCTTTGTTAATTCCAATAACATCTAAGGTCTCATTATTCTGCACATTAATAGCATTGGCTATAATAAAATTGGATATTCAACAAATCAACCTGCCATATATATTTACAAATTTCCTGTCAAGTGCAATGATTCATGCGACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACGGTTGAGCATGTAGGTTGGTGGTCACCAACTATTGTACGGGGTGAAGATCGTGAAGCTGTACACATCCCAAATCATAAGTTCACAGTGAATGTTGTGAGAAATCTCACTCAAAAAACTCATTGGCGCATCAAAACTCACCTTGCCATTAGTCACTTGGATGTCCATAAGATAAATAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCCCAAGTTGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCCGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACATCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTAGCCACACCAATCCGTACGGTGCAGAAAATGTATAGCGATACCGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAGTATCACCTAGACGCCCGTTGTTGCTAATTGAACCCTCGTACAAGATAAATGGAGAAGATAAAAAAAAAAGTCGTTCAGTTCAGACCAGTGGAGAGCGAGATGGGAAGGCCACAGTGCGGCCACAACCTGACTCCAAGGTGGATGCTAAAGTTGCTGCAACATCAGTATCTGACTCCAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGATGACCGCACTGCTTCAAAGTCATCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAAGACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTTCGGATCCGATATCTAATCATTTGACGGAGAAGATGTCTGGCAGAAAGCAACTGAATAATGCAATTGGAAATGTTGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTGCAGAGCCACCAACAAGACCTGCTCTAGAAGAAAATATTGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACGCTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAAATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

732

Amino Acids

80.86

Weight (kDa)

9.47

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	153 - 237	1.3e-37	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	274 - 344	2.9e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	349 - 435	1.2e-44	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	593
Acc16I	TGCGCA	1 cut(s)	1118
Acc36I	ACCTGC	2 cut(s)	819, 1993
Acc65I	GGTACC	1 cut(s)	60
AccB1I	GGYRCC	1 cut(s)	60
AccB7I	CCANNNNNTGG	1 cut(s)	356
AccI	GTMKAC	1 cut(s)	183
AccIII	TCCGGA	1 cut(s)	2106
AciI	CCGC	4 cut(s)	862, 1388, 1531, 1657
AclI	AACGTT	1 cut(s)	1185
AclWI	GGATC	8 cut(s)	527, 540, 637, 1293, 1679, 1778, 1791, 1927
AcoI	YGGCCR	2 cut(s)	262, 1532
AcsI	RAATTY	5 cut(s)	659, 829, 1636, 1738, 2116
AcyI	GRCGYC	3 cut(s)	360, 1426, 1631
AfaI	GTAC	5 cut(s)	62, 960, 984, 1339, 1454
AfiI	CCNNNNNNNGG	5 cut(s)	36, 37, 356, 1312, 1530
AjnI	CCWGG	2 cut(s)	1625, 2170
AloI	GAACNNNNNNTCC	2 cut(s)	1839, 1871
AluBI	AGCT	7 cut(s)	284, 441, 557, 980, 1205, 1274, 2165
AluI	AGCT	7 cut(s)	284, 441, 557, 980, 1205, 1274, 2165
Alw21I	GWGCWC	1 cut(s)	1865
Alw26I	GTCTC	3 cut(s)	139, 389, 754
AlwI	GGATC	8 cut(s)	527, 540, 637, 1293, 1679, 1778, 1791, 1927
AlwNI	CAGNNNCTG	1 cut(s)	1583
Aor13HI	TCCGGA	1 cut(s)	2106
AoxI	GGCC	6 cut(s)	188, 250, 262, 1314, 1521, 1532
ApeKI	GCWGC	3 cut(s)	438, 1568, 2165
ApoI	RAATTY	5 cut(s)	659, 829, 1636, 1738, 2116
ArsI	GACNNNNNNTTYG	2 cut(s)	821, 853
AseI	ATTAAT	1 cut(s)	767
Asp700I	GAANNNNTTC	2 cut(s)	885, 2062
Asp718I	GGTACC	1 cut(s)	60
AspLEI	GCGC	2 cut(s)	1050, 1119
AspS9I	GGNCC	3 cut(s)	4, 189, 1315
AsuHPI	GGTGA	5 cut(s)	939, 977, 1052, 1410, 1961
AvaII	GGWCC	1 cut(s)	4
BaeI	ACNNNNGTAYC	2 cut(s)	1563, 1596
BalI	TGGCCA	1 cut(s)	264
BamHI	GGATCC	2 cut(s)	532, 1783
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	2030
BauI	CACGAG	1 cut(s)	129
BbsI	GAAGAC	3 cut(s)	284, 1241, 2115
Bbv12I	GWGCWC	1 cut(s)	1865
BbvI	GCAGC	3 cut(s)	450, 1555, 2152
BccI	CCATC	4 cut(s)	30, 358, 1314, 1508
BceAI	ACGGC	1 cut(s)	593
BciT130I	CCWGG	2 cut(s)	1627, 2172
BclI	TGATCA	1 cut(s)	2152
BcoDI	GTCTC	3 cut(s)	139, 389, 754
BfaI	CTAG	6 cut(s)	149, 558, 1322, 1422, 1869, 1991
BfmI	CTRYAG	1 cut(s)	549
BfuAI	ACCTGC	2 cut(s)	819, 1993
BisI	GCNGC	4 cut(s)	439, 1532, 1569, 2166
BlsI	GCNGC	4 cut(s)	440, 1533, 1570, 2167
Bme1390I	CCNGG	2 cut(s)	1627, 2172
Bme18I	GGWCC	1 cut(s)	4
BmgT120I	GGNCC	3 cut(s)	4, 189, 1315
BmiI	GGNNCC	4 cut(s)	62, 348, 534, 1785
BmrFI	CCNGG	2 cut(s)	1627, 2172
BmrI	ACTGGG	1 cut(s)	1928
BmsI	GCATC	6 cut(s)	1059, 1546, 1612, 1738, 1947, 2074
BmuI	ACTGGG	1 cut(s)	1928
BpiI	GAAGAC	3 cut(s)	284, 1241, 2115
BplI	GAGNNNNNCTC	2 cut(s)	498, 530
BsaHI	GRCGYC	3 cut(s)	360, 1426, 1631
BsaI	GGTCTC	2 cut(s)	389, 754
BsaJI	CCNNGG	8 cut(s)	31, 288, 1548, 1614, 1647, 1773, 2008, 2019
BsaWI	WCCGGW	1 cut(s)	2106
BsaXI	ACNNNNNCTCC	4 cut(s)	477, 507, 1799, 1829
Bsc4I	CCNNNNNNNGG	5 cut(s)	36, 37, 356, 1312, 1530
Bse1I	ACTGG	6 cut(s)	16, 536, 684, 1500, 1934, 1961
Bse3DI	GCAATG	3 cut(s)	268, 516, 852
BseAI	TCCGGA	1 cut(s)	2106
BseBI	CCWGG	2 cut(s)	1627, 2172
BseDI	CCNNGG	8 cut(s)	31, 288, 1548, 1614, 1647, 1773, 2008, 2019
BseGI	GGATG	6 cut(s)	41, 987, 1087, 1561, 1657, 1928
BseLI	CCNNNNNNNGG	5 cut(s)	36, 37, 356, 1312, 1530
BseMI	GCAATG	3 cut(s)	268, 516, 852
BseMII	CTCAG	1 cut(s)	395
BseNI	ACTGG	6 cut(s)	16, 536, 684, 1500, 1934, 1961
BseXI	GCAGC	3 cut(s)	450, 1555, 2152
BsgI	GTGCAG	3 cut(s)	745, 1364, 2151
BshFI	GGCC	6 cut(s)	190, 252, 264, 1316, 1523, 1534
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1865
BsiSI	CCGG	1 cut(s)	2107
BsiWI	CGTACG	1 cut(s)	1337
BslFI	GGGAC	2 cut(s)	54, 107
BslI	CCNNNNNNNGG	5 cut(s)	36, 37, 356, 1312, 1530
BsmAI	GTCTC	3 cut(s)	139, 389, 754
BsmFI	GGGAC	2 cut(s)	54, 107
BsnI	GGCC	6 cut(s)	190, 252, 264, 1316, 1523, 1534
Bso31I	GGTCTC	2 cut(s)	389, 754
Bsp1286I	GDGCHC	2 cut(s)	1865, 2030
Bsp13I	TCCGGA	1 cut(s)	2106
Bsp1407I	TGTACA	1 cut(s)	982
Bsp19I	CCATGG	1 cut(s)	31
BspACI	CCGC	4 cut(s)	862, 1388, 1531, 1657
BspANI	GGCC	6 cut(s)	190, 252, 264, 1316, 1523, 1534
BspCNI	CTCAG	1 cut(s)	396
BspEI	TCCGGA	1 cut(s)	2106
BspHI	TCATGA	2 cut(s)	1209, 1246
BspLI	GGNNCC	4 cut(s)	62, 348, 534, 1785
BspMI	ACCTGC	2 cut(s)	819, 1993
BspPI	GGATC	8 cut(s)	527, 540, 637, 1293, 1679, 1778, 1791, 1927
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	2 cut(s)	389, 754
BsrDI	GCAATG	3 cut(s)	268, 516, 852
BsrGI	TGTACA	1 cut(s)	982
BsrI	ACTGG	6 cut(s)	16, 536, 684, 1500, 1934, 1961
BssECI	CCNNGG	8 cut(s)	31, 288, 1548, 1614, 1647, 1773, 2008, 2019
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	3 cut(s)	360, 1426, 1631
BssSI	CACGAG	1 cut(s)	129
BssT1I	CCWWGG	7 cut(s)	31, 1548, 1614, 1647, 1773, 2008, 2019
Bst1107I	GTATAC	1 cut(s)	184
Bst2BI	CACGAG	1 cut(s)	129
Bst2UI	CCWGG	2 cut(s)	1627, 2172
Bst4CI	ACNGT	9 cut(s)	72, 202, 292, 610, 925, 1009, 1342, 1376, 1528
Bst6I	CTCTTC	2 cut(s)	1162, 1245
BstACI	GRCGYC	3 cut(s)	360, 1426, 1631
BstAPI	GCANNNNNTGC	2 cut(s)	1159, 1901
BstAUI	TGTACA	1 cut(s)	982
BstDEI	CTNAG	4 cut(s)	404, 422, 744, 1292
BstDSI	CCRYGG	2 cut(s)	31, 288
BstEII	GGTNACC	1 cut(s)	945
BstF5I	GGATG	6 cut(s)	41, 987, 1087, 1561, 1657, 1928
BstHHI	GCGC	2 cut(s)	1050, 1119
BstMAI	GTCTC	3 cut(s)	139, 389, 754
BstMWI	GCNNNNNNNGC	5 cut(s)	270, 1159, 1322, 1565, 1901
BstNI	CCWGG	2 cut(s)	1627, 2172
BstNSI	RCATGY	2 cut(s)	935, 1117
BstPI	GGTNACC	1 cut(s)	945
BstSCI	CCNGG	2 cut(s)	1625, 2170
BstSFI	CTRYAG	1 cut(s)	549
BstV1I	GCAGC	3 cut(s)	450, 1555, 2152
BstV2I	GAAGAC	3 cut(s)	284, 1241, 2115
BstX2I	RGATCY	3 cut(s)	532, 1285, 1783
BstXI	CCANNNNNNTGG	1 cut(s)	260
BstYI	RGATCY	3 cut(s)	532, 1285, 1783
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	6 cut(s)	190, 252, 264, 1316, 1523, 1534
BtgI	CCRYGG	2 cut(s)	31, 288
BtsCI	GGATG	6 cut(s)	41, 987, 1087, 1561, 1657, 1928
BtsI	GCAGTG	2 cut(s)	114, 1659
BtsIMutI	CAGTG	8 cut(s)	9, 114, 691, 1014, 1507, 1533, 1659, 1941
BveI	ACCTGC	2 cut(s)	819, 1993
CaiI	CAGNNNCTG	1 cut(s)	1583
CciI	TCATGA	2 cut(s)	1209, 1246
CfoI	GCGC	2 cut(s)	1050, 1119
Cfr13I	GGNCC	3 cut(s)	4, 189, 1315
CseI	GACGC	4 cut(s)	349, 389, 1434, 1639
Csp6I	GTAC	5 cut(s)	61, 959, 983, 1338, 1453
CspCI	CAANNNNNGTGG	2 cut(s)	1897, 1932
CviAII	CATG	8 cut(s)	32, 218, 854, 932, 1114, 1210, 1247, 1282
CviQI	GTAC	5 cut(s)	61, 959, 983, 1338, 1453
DdeI	CTNAG	4 cut(s)	404, 422, 744, 1292
EaeI	YGGCCR	2 cut(s)	262, 1532
Eam1104I	CTCTTC	2 cut(s)	1162, 1245
EarI	CTCTTC	2 cut(s)	1162, 1245
Eco130I	CCWWGG	7 cut(s)	31, 1548, 1614, 1647, 1773, 2008, 2019
Eco24I	GRGCYC	1 cut(s)	2030
Eco31I	GGTCTC	2 cut(s)	389, 754
Eco32I	GATATC	2 cut(s)	215, 1791
Eco47I	GGWCC	1 cut(s)	4
Eco91I	GGTNACC	1 cut(s)	945
EcoO65I	GGTNACC	1 cut(s)	945
EcoRII	CCWGG	2 cut(s)	1625, 2170
EcoRV	GATATC	2 cut(s)	215, 1791
EcoT14I	CCWWGG	7 cut(s)	31, 1548, 1614, 1647, 1773, 2008, 2019
EcoT38I	GRGCYC	1 cut(s)	2030
ErhI	CCWWGG	7 cut(s)	31, 1548, 1614, 1647, 1773, 2008, 2019
FaeI	CATG	8 cut(s)	35, 221, 857, 935, 1117, 1213, 1250, 1285
FaqI	GGGAC	2 cut(s)	54, 107
FatI	CATG	8 cut(s)	31, 217, 853, 931, 1113, 1209, 1246, 1281
FauI	CCCGC	1 cut(s)	869
FauNDI	CATATG	2 cut(s)	307, 377
FbaI	TGATCA	1 cut(s)	2152
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	4 cut(s)	439, 1532, 1569, 2166
FokI	GGATG	6 cut(s)	48, 974, 1094, 1568, 1664, 1915
FriOI	GRGCYC	1 cut(s)	2030
Fsp4HI	GCNGC	4 cut(s)	439, 1532, 1569, 2166
FspBI	CTAG	6 cut(s)	149, 558, 1322, 1422, 1869, 1991
FspI	TGCGCA	1 cut(s)	1118
GlaI	GCGC	2 cut(s)	1049, 1118
GluI	GCNGC	4 cut(s)	439, 1532, 1569, 2166
HaeIII	GGCC	6 cut(s)	190, 252, 264, 1316, 1523, 1534
HapII	CCGG	1 cut(s)	2107
HgaI	GACGC	4 cut(s)	349, 389, 1434, 1639
HhaI	GCGC	2 cut(s)	1050, 1119
Hin1I	GRCGYC	3 cut(s)	360, 1426, 1631
Hin1II	CATG	8 cut(s)	35, 221, 857, 935, 1117, 1213, 1250, 1285
Hin6I	GCGC	2 cut(s)	1048, 1117
HinP1I	GCGC	2 cut(s)	1048, 1117
HpaII	CCGG	1 cut(s)	2107
HphI	GGTGA	5 cut(s)	939, 977, 1052, 1410, 1961
Hpy166II	GTNNAC	3 cut(s)	184, 985, 1005
Hpy8I	GTNNAC	3 cut(s)	184, 985, 1005
HpyAV	CCTTC	9 cut(s)	84, 896, 1513, 1689, 1940, 2048, 2051, 2079, 2109
HpyCH4III	ACNGT	9 cut(s)	72, 202, 292, 610, 925, 1009, 1342, 1376, 1528
HpyCH4IV	ACGT	1 cut(s)	1185
HpyF10VI	GCNNNNNNNGC	5 cut(s)	270, 1159, 1322, 1565, 1901
HpyF3I	CTNAG	4 cut(s)	404, 422, 744, 1292
HpySE526I	ACGT	1 cut(s)	1185
Hsp92I	GRCGYC	3 cut(s)	360, 1426, 1631
Hsp92II	CATG	8 cut(s)	35, 221, 857, 935, 1117, 1213, 1250, 1285
HspAI	GCGC	2 cut(s)	1048, 1117
Kpn2I	TCCGGA	1 cut(s)	2106
KpnI	GGTACC	1 cut(s)	64
Ksp22I	TGATCA	1 cut(s)	2152
LmnI	GCTCC	2 cut(s)	498, 506
Lsp1109I	GCAGC	3 cut(s)	450, 1555, 2152
LweI	GCATC	6 cut(s)	1059, 1546, 1612, 1738, 1947, 2074
MaeI	CTAG	6 cut(s)	149, 558, 1322, 1422, 1869, 1991
MaeII	ACGT	1 cut(s)	1185
MaeIII	GTNAC	6 cut(s)	241, 466, 674, 945, 1073, 1186
MfeI	CAATTG	1 cut(s)	1842
MflI	RGATCY	3 cut(s)	532, 1285, 1783
MhlI	GDGCHC	2 cut(s)	1865, 2030
MlsI	TGGCCA	1 cut(s)	264
MluNI	TGGCCA	1 cut(s)	264
MlyI	GAGTC	7 cut(s)	141, 689, 1305, 1385, 1538, 1580, 1763
MmeI	TCCRAC	1 cut(s)	670
MnlI	CCTC	7 cut(s)	17, 339, 1149, 1459, 1897, 2017, 2044
Mox20I	TGGCCA	1 cut(s)	264
MroI	TCCGGA	1 cut(s)	2106
MroXI	GAANNNNTTC	2 cut(s)	885, 2062
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	5 cut(s)	75, 225, 570, 728, 767
MslI	CAYNNNNRTG	3 cut(s)	717, 1080, 2082
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	2 cut(s)	441, 2165
MspI	CCGG	1 cut(s)	2107
MspR9I	CCNGG	2 cut(s)	1627, 2172
MunI	CAATTG	1 cut(s)	1842
MvaI	CCWGG	2 cut(s)	1627, 2172
MwoI	GCNNNNNNNGC	5 cut(s)	270, 1159, 1322, 1565, 1901
NcoI	CCATGG	1 cut(s)	31
NdeI	CATATG	2 cut(s)	307, 377
NlaIII	CATG	8 cut(s)	35, 221, 857, 935, 1117, 1213, 1250, 1285
NlaIV	GGNNCC	4 cut(s)	62, 348, 534, 1785
NmuCI	GTSAC	2 cut(s)	945, 1073
NsbI	TGCGCA	1 cut(s)	1118
NspI	RCATGY	2 cut(s)	935, 1117
PagI	TCATGA	2 cut(s)	1209, 1246
PcsI	WCGNNNNNNNCGW	1 cut(s)	1191
PdmI	GAANNNNTTC	2 cut(s)	885, 2062
PfeI	GAWTC	7 cut(s)	52, 145, 152, 850, 886, 1135, 1763
Pfl23II	CGTACG	1 cut(s)	1337
PflMI	CCANNNNNTGG	1 cut(s)	356
PkrI	GCNGC	4 cut(s)	440, 1533, 1570, 2167
PleI	GAGTC	7 cut(s)	140, 688, 1304, 1385, 1538, 1580, 1763
PpsI	GAGTC	7 cut(s)	140, 688, 1304, 1385, 1538, 1580, 1763
PshBI	ATTAAT	1 cut(s)	767
PsiI	TTATAA	1 cut(s)	593
Psp1406I	AACGTT	1 cut(s)	1185
Psp6I	CCWGG	2 cut(s)	1625, 2170
PspEI	GGTNACC	1 cut(s)	945
PspGI	CCWGG	2 cut(s)	1625, 2170
PspLI	CGTACG	1 cut(s)	1337
PspN4I	GGNNCC	4 cut(s)	62, 348, 534, 1785
PspPI	GGNCC	3 cut(s)	4, 189, 1315
PstNI	CAGNNNCTG	1 cut(s)	1583
PsuI	RGATCY	3 cut(s)	532, 1285, 1783
PvuII	CAGCTG	2 cut(s)	441, 2165
RsaI	GTAC	5 cut(s)	62, 960, 984, 1339, 1454
RsaNI	GTAC	5 cut(s)	61, 959, 983, 1338, 1453
RseI	CAYNNNNRTG	3 cut(s)	717, 1080, 2082
SaqAI	TTAA	5 cut(s)	75, 225, 570, 728, 767
SatI	GCNGC	4 cut(s)	439, 1532, 1569, 2166
Sau96I	GGNCC	3 cut(s)	4, 189, 1315
SchI	GAGTC	7 cut(s)	141, 689, 1305, 1385, 1538, 1580, 1763
ScrFI	CCNGG	2 cut(s)	1627, 2172
SduI	GDGCHC	2 cut(s)	1865, 2030
SfaNI	GCATC	6 cut(s)	1059, 1546, 1612, 1738, 1947, 2074
SfcI	CTRYAG	1 cut(s)	549
SinI	GGWCC	1 cut(s)	4
SmiMI	CAYNNNNRTG	3 cut(s)	717, 1080, 2082
SsiI	CCGC	4 cut(s)	862, 1388, 1531, 1657
SspI	AATATT	1 cut(s)	2002
SspMI	CTAG	6 cut(s)	149, 558, 1322, 1422, 1869, 1991
StyD4I	CCNGG	2 cut(s)	1625, 2170
StyI	CCWWGG	7 cut(s)	31, 1548, 1614, 1647, 1773, 2008, 2019
TaaI	ACNGT	9 cut(s)	72, 202, 292, 610, 925, 1009, 1342, 1376, 1528
TaiI	ACGT	1 cut(s)	1188
TaqI	TCGA	1 cut(s)	1724
TatI	WGTACW	1 cut(s)	982
TauI	GCSGC	1 cut(s)	1534
TfiI	GAWTC	7 cut(s)	52, 145, 152, 850, 886, 1135, 1763
Tru1I	TTAA	5 cut(s)	75, 225, 570, 728, 767
Tru9I	TTAA	5 cut(s)	75, 225, 570, 728, 767
TscAI	CASTG	8 cut(s)	16, 121, 691, 1014, 1507, 1533, 1666, 1941
TseFI	GTSAC	2 cut(s)	945, 1073
TseI	GCWGC	3 cut(s)	438, 1568, 2165
Tsp45I	GTSAC	2 cut(s)	945, 1073
TspDTI	ATGAA	5 cut(s)	842, 894, 924, 1263, 2061
TspGWI	ACGGA	2 cut(s)	1325, 1820
TspRI	CASTG	8 cut(s)	16, 121, 691, 1014, 1507, 1533, 1666, 1941
Van91I	CCANNNNNTGG	1 cut(s)	356
VpaK11BI	GGWCC	1 cut(s)	4
VspI	ATTAAT	1 cut(s)	767
XapI	RAATTY	5 cut(s)	659, 829, 1636, 1738, 2116
XbaI	TCTAGA	2 cut(s)	148, 1990
XceI	RCATGY	2 cut(s)	935, 1117
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1075
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	885, 2062
XspI	CTAG	6 cut(s)	149, 558, 1322, 1422, 1869, 1991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.