Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

23521102 .. 23528725

Gene Structure 7624 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG236600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGATCCTCACTGGTTCGTTGCAGTTATCCCATGGATTGGGACTTTGCAAGAATCAGGGGTATCAGAAACAGTTTAAGAGGGGAATAGGATGGGACAAATTACATCTATCAAGCACTGCCCTTACATCTCGAGTCTCGTTTCAGAAACTGGATTCTTGGAGTATTGGTTTATCAGAGAATGTATACAGGCCCATACATACTGTACCTCACAGATATCATGTGTTTAAGTGCCATTCATCTGTAACATCAGGCCAATCTTTTGGCCAACATGGCTTGAAGACAGCTACCATGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCGTCATTATATTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGTAACACACACTATGTTACGAACTCATATATTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATATGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTTTGGATTTTATAAGATCGCTGTCAACTGTTCTGTCTTTTGCCTATTGTTTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCTGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTTACTGCAGGAGGTCTTGGGACAGTCTTATTGACTCTGGCTGGTCGTGAGATATTTACAAATTTCCTGTCAAGTGCAATGATTCATGCCACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACGGTTGAGCATGTAGGTTGGTGGTCGCCAACTATTGTAAGAGGTGAAGATCGTGAAGCTGTACACATCCCAAACCATAAGTTCACGGTGAATGTTGTGAGAAATCTCACTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATTAGTCACTTGGATGTCCATAAGATAAATAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCTCAAGTGGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCTGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACCTCACGTCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAGCGATACTGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAGTATCACCTAGACGCCCGTTGTTGCTAATTGAACCCTCGTACAAGATAAATGGAGAAGATAAAAAAAAAAGTCGTTCAGTGCAGACCAGTGGAGAGCGAGATGGGAAGGCCACAGTGCGGCCACAACCTGACTCCAAGGTCGATGCTAAAGTTGCTACAACATCAGTATCTGACTCCAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACAACCCCACTGCTTCAAAGTCATCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAATACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCGGACCCGATATCTAATCATTTGACGGAGAAGATGTCTGGCAGAAAGCAACTGAATAATGCAAGTGGAAATGTAGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTGCAGAGCCACCAACAAGAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACACTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAAATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

742

Amino Acids

81.82

Weight (kDa)

9.59

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	153 - 282	3.9e-72	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	284 - 354	1.8e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	359 - 445	1.1e-43	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	593
AasI	GACNNNNNNGTC	1 cut(s)	1580
Acc16I	TGCGCA	1 cut(s)	1148
Acc36I	ACCTGC	1 cut(s)	2026
AccB7I	CCANNNNNTGG	2 cut(s)	37, 356
AccI	GTMKAC	1 cut(s)	183
AccIII	TCCGGA	1 cut(s)	2139
AciI	CCGC	4 cut(s)	892, 1418, 1561, 1811
AclI	AACGTT	1 cut(s)	1215
AclWI	GGATC	6 cut(s)	527, 540, 637, 1323, 1709, 1957
AcoI	YGGCCR	2 cut(s)	262, 1562
AcsI	RAATTY	5 cut(s)	659, 859, 1666, 1768, 2149
AcyI	GRCGYC	3 cut(s)	360, 1456, 1661
AfaI	GTAC	5 cut(s)	204, 731, 1014, 1369, 1484
AfiI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
AgsI	TTSAA	9 cut(s)	277, 298, 340, 485, 920, 1475, 1671, 1698, 2160
AhdI	GACNNNNNGTC	1 cut(s)	1272
AjiI	CACGTC	1 cut(s)	1274
AjnI	CCWGG	2 cut(s)	1655, 2203
AloI	GAACNNNNNNTCC	2 cut(s)	1869, 1901
AluBI	AGCT	8 cut(s)	284, 441, 557, 725, 1010, 1235, 1304, 2198
AluI	AGCT	8 cut(s)	284, 441, 557, 725, 1010, 1235, 1304, 2198
Alw21I	GWGCWC	1 cut(s)	1895
Alw26I	GTCTC	2 cut(s)	139, 389
AlwI	GGATC	6 cut(s)	527, 540, 637, 1323, 1709, 1957
AlwNI	CAGNNNCTG	2 cut(s)	148, 1613
Ama87I	CYCGRG	1 cut(s)	129
Aor13HI	TCCGGA	1 cut(s)	2139
AoxI	GGCC	6 cut(s)	188, 250, 262, 1344, 1551, 1562
ApeKI	GCWGC	4 cut(s)	438, 749, 1808, 2198
ApoI	RAATTY	5 cut(s)	659, 859, 1666, 1768, 2149
ArsI	GACNNNNNNTTYG	2 cut(s)	851, 883
Asp700I	GAANNNNTTC	2 cut(s)	915, 2095
AspLEI	GCGC	1 cut(s)	1149
AspS9I	GGNCC	3 cut(s)	189, 1345, 1813
AsuHPI	GGTGA	5 cut(s)	1007, 1051, 1082, 1440, 1991
AvaI	CYCGRG	1 cut(s)	129
AvaII	GGWCC	1 cut(s)	1813
BaeI	ACNNNNGTAYC	2 cut(s)	1593, 1626
BalI	TGGCCA	1 cut(s)	264
BamHI	GGATCC	1 cut(s)	532
BanII	GRGCYC	1 cut(s)	2063
BbsI	GAAGAC	4 cut(s)	284, 1271, 2020, 2148
Bbv12I	GWGCWC	1 cut(s)	1895
BbvI	GCAGC	4 cut(s)	450, 736, 1795, 2185
BccI	CCATC	4 cut(s)	84, 358, 1344, 1538
BceAI	ACGGC	1 cut(s)	593
BciT130I	CCWGG	2 cut(s)	1657, 2205
BclI	TGATCA	1 cut(s)	2185
BcoDI	GTCTC	2 cut(s)	139, 389
BfaI	CTAG	5 cut(s)	558, 776, 1452, 1899, 2024
BfmI	CTRYAG	2 cut(s)	549, 804
BfuAI	ACCTGC	1 cut(s)	2026
BglI	GCCNNNNNGGC	1 cut(s)	270
BisI	GCNGC	5 cut(s)	439, 750, 1562, 1809, 2199
BlsI	GCNGC	5 cut(s)	440, 751, 1563, 1810, 2200
Bme1390I	CCNGG	2 cut(s)	1657, 2205
Bme18I	GGWCC	1 cut(s)	1813
BmeRI	GACNNNNNGTC	1 cut(s)	1272
BmeT110I	CYCGRG	1 cut(s)	129
BmgBI	CACGTC	1 cut(s)	1274
BmgT120I	GGNCC	3 cut(s)	189, 1345, 1813
BmiI	GGNNCC	3 cut(s)	348, 534, 1815
BmrFI	CCNGG	2 cut(s)	1657, 2205
BmrI	ACTGGG	1 cut(s)	1958
BmsI	GCATC	5 cut(s)	1576, 1642, 1768, 1977, 2107
BmuI	ACTGGG	1 cut(s)	1958
BpiI	GAAGAC	4 cut(s)	284, 1271, 2020, 2148
BplI	GAGNNNNNCTC	2 cut(s)	498, 530
BpuEI	CTTGAG	1 cut(s)	1155
BsaHI	GRCGYC	3 cut(s)	360, 1456, 1661
BsaI	GGTCTC	1 cut(s)	389
BsaJI	CCNNGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
BsaWI	WCCGGW	1 cut(s)	2139
BsaXI	ACNNNNNCTCC	6 cut(s)	151, 181, 477, 507, 1829, 1859
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
Bse1I	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 1964, 1991
Bse3DI	GCAATG	2 cut(s)	516, 882
BseAI	TCCGGA	1 cut(s)	2139
BseBI	CCWGG	2 cut(s)	1657, 2205
BseDI	CCNNGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
BseGI	GGATG	4 cut(s)	95, 1017, 1117, 1958
BseLI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
BseMI	GCAATG	2 cut(s)	516, 882
BseMII	CTCAG	1 cut(s)	395
BseNI	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 1964, 1991
BseXI	GCAGC	4 cut(s)	450, 736, 1795, 2185
BseYI	CCCAGC	1 cut(s)	716
BsgI	GTGCAG	3 cut(s)	1394, 1544, 2184
BshFI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BsiHKAI	GWGCWC	1 cut(s)	1895
BsiHKCI	CYCGRG	1 cut(s)	129
BsiSI	CCGG	1 cut(s)	2140
BslFI	GGGAC	3 cut(s)	54, 107, 832
BslI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
BsmAI	GTCTC	2 cut(s)	139, 389
BsmFI	GGGAC	3 cut(s)	54, 107, 832
BsnI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
Bso31I	GGTCTC	1 cut(s)	389
BsoBI	CYCGRG	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	1895, 2063
Bsp13I	TCCGGA	1 cut(s)	2139
Bsp1407I	TGTACA	1 cut(s)	1012
Bsp19I	CCATGG	2 cut(s)	31, 288
BspACI	CCGC	4 cut(s)	892, 1418, 1561, 1811
BspANI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BspCNI	CTCAG	1 cut(s)	396
BspEI	TCCGGA	1 cut(s)	2139
BspHI	TCATGA	2 cut(s)	1239, 1276
BspLI	GGNNCC	3 cut(s)	348, 534, 1815
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	1 cut(s)	2026
BspPI	GGATC	6 cut(s)	527, 540, 637, 1323, 1709, 1957
BspTNI	GGTCTC	1 cut(s)	389
BsrDI	GCAATG	2 cut(s)	516, 882
BsrGI	TGTACA	1 cut(s)	1012
BsrI	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 1964, 1991
BssECI	CCNNGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	3 cut(s)	360, 1456, 1661
BssT1I	CCWWGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
Bst1107I	GTATAC	1 cut(s)	184
Bst2UI	CCWGG	2 cut(s)	1657, 2205
Bst4CI	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
Bst6I	CTCTTC	2 cut(s)	1192, 1275
BstACI	GRCGYC	3 cut(s)	360, 1456, 1661
BstAPI	GCANNNNNTGC	2 cut(s)	1189, 1931
BstAUI	TGTACA	1 cut(s)	1012
BstDEI	CTNAG	3 cut(s)	404, 422, 1322
BstDSI	CCRYGG	2 cut(s)	31, 288
BstF5I	GGATG	4 cut(s)	95, 1017, 1117, 1958
BstHHI	GCGC	1 cut(s)	1149
BstMAI	GTCTC	2 cut(s)	139, 389
BstMWI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 1931
BstNI	CCWGG	2 cut(s)	1657, 2205
BstNSI	RCATGY	2 cut(s)	965, 1147
BstSCI	CCNGG	2 cut(s)	1655, 2203
BstSFI	CTRYAG	2 cut(s)	549, 804
BstV1I	GCAGC	4 cut(s)	450, 736, 1795, 2185
BstV2I	GAAGAC	4 cut(s)	284, 1271, 2020, 2148
BstX2I	RGATCY	2 cut(s)	532, 1315
BstXI	CCANNNNNNTGG	2 cut(s)	260, 716
BstYI	RGATCY	2 cut(s)	532, 1315
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BtgI	CCRYGG	2 cut(s)	31, 288
BtrI	CACGTC	1 cut(s)	1274
BtsCI	GGATG	4 cut(s)	95, 1017, 1117, 1958
BtsI	GCAGTG	2 cut(s)	114, 1689
BveI	ACCTGC	1 cut(s)	2026
CaiI	CAGNNNCTG	2 cut(s)	148, 1613
CciI	TCATGA	2 cut(s)	1239, 1276
CfoI	GCGC	1 cut(s)	1149
Cfr13I	GGNCC	3 cut(s)	189, 1345, 1813
CseI	GACGC	4 cut(s)	349, 389, 1464, 1669
Csp6I	GTAC	5 cut(s)	203, 730, 1013, 1368, 1483
CspCI	CAANNNNNGTGG	2 cut(s)	1927, 1962
CviQI	GTAC	5 cut(s)	203, 730, 1013, 1368, 1483
DdeI	CTNAG	3 cut(s)	404, 422, 1322
DrdI	GACNNNNNNGTC	1 cut(s)	1580
DriI	GACNNNNNGTC	1 cut(s)	1272
DseDI	GACNNNNNNGTC	1 cut(s)	1580
EaeI	YGGCCR	2 cut(s)	262, 1562
Eam1104I	CTCTTC	2 cut(s)	1192, 1275
Eam1105I	GACNNNNNGTC	1 cut(s)	1272
EarI	CTCTTC	2 cut(s)	1192, 1275
Eco130I	CCWWGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
Eco24I	GRGCYC	1 cut(s)	2063
Eco31I	GGTCTC	1 cut(s)	389
Eco32I	GATATC	2 cut(s)	215, 1821
Eco47I	GGWCC	1 cut(s)	1813
Eco88I	CYCGRG	1 cut(s)	129
EcoRII	CCWGG	2 cut(s)	1655, 2203
EcoRV	GATATC	2 cut(s)	215, 1821
EcoT14I	CCWWGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
EcoT38I	GRGCYC	1 cut(s)	2063
ErhI	CCWWGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
FaqI	GGGAC	3 cut(s)	54, 107, 832
FauI	CCCGC	1 cut(s)	899
FauNDI	CATATG	3 cut(s)	307, 377, 518
FbaI	TGATCA	1 cut(s)	2185
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	5 cut(s)	439, 750, 1562, 1809, 2199
FokI	GGATG	4 cut(s)	102, 1004, 1124, 1945
FriOI	GRGCYC	1 cut(s)	2063
Fsp4HI	GCNGC	5 cut(s)	439, 750, 1562, 1809, 2199
FspBI	CTAG	5 cut(s)	558, 776, 1452, 1899, 2024
FspI	TGCGCA	1 cut(s)	1148
GlaI	GCGC	1 cut(s)	1148
GluI	GCNGC	5 cut(s)	439, 750, 1562, 1809, 2199
GsaI	CCCAGC	1 cut(s)	720
HaeIII	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
HapII	CCGG	1 cut(s)	2140
HgaI	GACGC	4 cut(s)	349, 389, 1464, 1669
HhaI	GCGC	1 cut(s)	1149
Hin1I	GRCGYC	3 cut(s)	360, 1456, 1661
Hin6I	GCGC	1 cut(s)	1147
HinP1I	GCGC	1 cut(s)	1147
HincII	GTYRAC	1 cut(s)	606
HindII	GTYRAC	1 cut(s)	606
HpaII	CCGG	1 cut(s)	2140
HphI	GGTGA	5 cut(s)	1007, 1051, 1082, 1440, 1991
Hpy166II	GTNNAC	5 cut(s)	184, 606, 730, 1015, 1035
Hpy8I	GTNNAC	5 cut(s)	184, 606, 730, 1015, 1035
HpyAV	CCTTC	8 cut(s)	926, 1543, 1719, 1970, 2081, 2084, 2112, 2142
HpyCH4III	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
HpyCH4IV	ACGT	2 cut(s)	1215, 1273
HpyF10VI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 1931
HpyF3I	CTNAG	3 cut(s)	404, 422, 1322
HpySE526I	ACGT	2 cut(s)	1215, 1273
Hsp92I	GRCGYC	3 cut(s)	360, 1456, 1661
HspAI	GCGC	1 cut(s)	1147
Kpn2I	TCCGGA	1 cut(s)	2139
Ksp22I	TGATCA	1 cut(s)	2185
LmnI	GCTCC	2 cut(s)	498, 506
Lsp1109I	GCAGC	4 cut(s)	450, 736, 1795, 2185
LweI	GCATC	5 cut(s)	1576, 1642, 1768, 1977, 2107
MaeI	CTAG	5 cut(s)	558, 776, 1452, 1899, 2024
MaeII	ACGT	2 cut(s)	1215, 1273
MaeIII	GTNAC	6 cut(s)	241, 452, 466, 674, 1103, 1216
MflI	RGATCY	2 cut(s)	532, 1315
MhlI	GDGCHC	2 cut(s)	1895, 2063
MlsI	TGGCCA	1 cut(s)	264
MluCI	AATT	9 cut(s)	98, 341, 659, 710, 859, 1470, 1666, 1768, 2149
MluNI	TGGCCA	1 cut(s)	264
MlyI	GAGTC	8 cut(s)	141, 689, 826, 1335, 1415, 1568, 1610, 1793
MmeI	TCCRAC	1 cut(s)	670
Mox20I	TGGCCA	1 cut(s)	264
MroI	TCCGGA	1 cut(s)	2139
MroXI	GAANNNNTTC	2 cut(s)	915, 2095
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	3 cut(s)	75, 225, 570
MslI	CAYNNNNRTG	2 cut(s)	1110, 2115
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	2 cut(s)	441, 2198
MspI	CCGG	1 cut(s)	2140
MspR9I	CCNGG	2 cut(s)	1657, 2205
MvaI	CCWGG	2 cut(s)	1657, 2205
MwoI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 1931
NcoI	CCATGG	2 cut(s)	31, 288
NdeI	CATATG	3 cut(s)	307, 377, 518
NlaIV	GGNNCC	3 cut(s)	348, 534, 1815
NmuCI	GTSAC	1 cut(s)	1103
NsbI	TGCGCA	1 cut(s)	1148
NspI	RCATGY	2 cut(s)	965, 1147
PaeR7I	CTCGAG	1 cut(s)	129
PagI	TCATGA	2 cut(s)	1239, 1276
PdmI	GAANNNNTTC	2 cut(s)	915, 2095
PfeI	GAWTC	6 cut(s)	52, 152, 880, 916, 1165, 1793
PflMI	CCANNNNNTGG	2 cut(s)	37, 356
PkrI	GCNGC	5 cut(s)	440, 751, 1563, 1810, 2200
PleI	GAGTC	8 cut(s)	140, 688, 826, 1334, 1415, 1568, 1610, 1793
PpsI	GAGTC	8 cut(s)	140, 688, 826, 1334, 1415, 1568, 1610, 1793
PsiI	TTATAA	1 cut(s)	593
Psp1406I	AACGTT	1 cut(s)	1215
Psp6I	CCWGG	2 cut(s)	1655, 2203
PspFI	CCCAGC	1 cut(s)	716
PspGI	CCWGG	2 cut(s)	1655, 2203
PspN4I	GGNNCC	3 cut(s)	348, 534, 1815
PspPI	GGNCC	3 cut(s)	189, 1345, 1813
PstI	CTGCAG	1 cut(s)	808
PstNI	CAGNNNCTG	2 cut(s)	148, 1613
PsuI	RGATCY	2 cut(s)	532, 1315
PvuII	CAGCTG	2 cut(s)	441, 2198
RsaI	GTAC	5 cut(s)	204, 731, 1014, 1369, 1484
RsaNI	GTAC	5 cut(s)	203, 730, 1013, 1368, 1483
RseI	CAYNNNNRTG	2 cut(s)	1110, 2115
SaqAI	TTAA	3 cut(s)	75, 225, 570
SatI	GCNGC	5 cut(s)	439, 750, 1562, 1809, 2199
Sau96I	GGNCC	3 cut(s)	189, 1345, 1813
SchI	GAGTC	8 cut(s)	141, 689, 826, 1335, 1415, 1568, 1610, 1793
ScrFI	CCNGG	2 cut(s)	1657, 2205
SduI	GDGCHC	2 cut(s)	1895, 2063
SfaNI	GCATC	5 cut(s)	1576, 1642, 1768, 1977, 2107
SfcI	CTRYAG	2 cut(s)	549, 804
Sfr274I	CTCGAG	1 cut(s)	129
SinI	GGWCC	1 cut(s)	1813
SlaI	CTCGAG	1 cut(s)	129
SmiMI	CAYNNNNRTG	2 cut(s)	1110, 2115
SmlI	CTYRAG	2 cut(s)	129, 1170
SmoI	CTYRAG	2 cut(s)	129, 1170
Sse9I	AATT	9 cut(s)	98, 341, 659, 710, 859, 1470, 1666, 1768, 2149
SsiI	CCGC	4 cut(s)	892, 1418, 1561, 1811
SspMI	CTAG	5 cut(s)	558, 776, 1452, 1899, 2024
StyD4I	CCNGG	2 cut(s)	1655, 2203
StyI	CCWWGG	8 cut(s)	31, 288, 1578, 1644, 1677, 1803, 2041, 2052
TaaI	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
TaiI	ACGT	2 cut(s)	1218, 1276
TaqI	TCGA	3 cut(s)	130, 1584, 1754
TasI	AATT	9 cut(s)	98, 341, 659, 710, 859, 1470, 1666, 1768, 2149
TatI	WGTACW	2 cut(s)	729, 1012
TauI	GCSGC	1 cut(s)	1564
TfiI	GAWTC	6 cut(s)	52, 152, 880, 916, 1165, 1793
Tru1I	TTAA	3 cut(s)	75, 225, 570
Tru9I	TTAA	3 cut(s)	75, 225, 570
TseFI	GTSAC	1 cut(s)	1103
TseI	GCWGC	4 cut(s)	438, 749, 1808, 2198
Tsp45I	GTSAC	1 cut(s)	1103
TspDTI	ATGAA	7 cut(s)	225, 754, 872, 924, 954, 1293, 2094
TspGWI	ACGGA	2 cut(s)	1355, 1850
Van91I	CCANNNNNTGG	2 cut(s)	37, 356
VpaK11BI	GGWCC	1 cut(s)	1813
XapI	RAATTY	5 cut(s)	659, 859, 1666, 1768, 2149
XbaI	TCTAGA	1 cut(s)	2023
XceI	RCATGY	2 cut(s)	965, 1147
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1105
XhoI	CTCGAG	1 cut(s)	129
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	915, 2095
XspI	CTAG	5 cut(s)	558, 776, 1452, 1899, 2024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.