Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

22796229 .. 22803187

Gene Structure 6959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ48482

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGTCCTCACTGGTTCGTTGCAGTTATCCCATGGATTGGGACTTTGCAAGAATCAGGGGTACCAGAAACAGTTTAAGAGGGGAATAGGATGGGACAAATTACATCTATCAAGCACTGCCCTTACATCTCGAGTCTTGTTTCAGAAACTGGATTCTTGGAGTATTGGTTTATCAGAGAATGTATACAGGCCCATACATACTGTACCTCACAGATATCATGTGTTTAAGTGCCATTCATCTGTAACATCAGGCCAATCTTTTGGCCAACATGGCTTGAAGACAGCTACCATGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCGTCATTATATTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGTAACACACACTATGTTACGAACTCATATATTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATTTGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTTTGGATTTTATAAGATCGCTGTCAACTGTTCTGTCTTTTGCCTATTGTTTATCAAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCTGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTTACTGCAGGAGGTCTTGGGACAGTCTTATTGACTCTGGCTGGTCGTGAGATATTTACAAATTTCCTGTCAAGTGCAATGATTCATGCCACCCGCCCTTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACGGTTGAGCATGTAGGTTGGTGGTCACCAACTATTGTAAGAGGTGAAGATCGTGAAGCTGTACACATCCCAAACCATAAGTTCACAGTGAATGTTGTGAGAAATCTCACTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATTAGTCACTTGGATGTCCATAAGATAAATAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCTCAAGTGGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCTGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACGTCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAGCGATACCGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAGTATCACCTAGACGCCCGTTGTTGCTAATTGAACCCTCGTACAAGATAAATGGAGAAGATAAAAAAAAAAGTCGTTCAGTGCAGACCAGTGGAGAGCGAGATGGGAAGGCCACAGTGCGGCCACAACCTGACTCCAAGGTCGATGCTAAAGTTGCTGCAACATCAGTATCTGACTCCAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACAACCCCACTGCTTCAAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACAACCCCACTGCTTCAAAGACAACACCAACATCTTCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAATACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCGGACCCGATATCTAATCATTTGACGGAGAAGATGTCTGGTAGAAAGCAACTGAATAATGCAAGTGGAAATGTAGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTACAGAGCCACCAACAAGAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACACTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAAATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

772

Amino Acids

84.93

Weight (kDa)

9.6

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	153 - 282	4.1e-72	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	284 - 354	1.9e-10	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	359 - 445	1.1e-43	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	593
AasI	GACNNNNNNGTC	1 cut(s)	1580
Acc16I	TGCGCA	1 cut(s)	1148
Acc36I	ACCTGC	1 cut(s)	2116
Acc65I	GGTACC	1 cut(s)	60
AccB1I	GGYRCC	1 cut(s)	60
AccB7I	CCANNNNNTGG	2 cut(s)	37, 356
AccI	GTMKAC	1 cut(s)	183
AccIII	TCCGGA	1 cut(s)	2229
AciI	CCGC	4 cut(s)	892, 1418, 1561, 1901
AclI	AACGTT	1 cut(s)	1215
AclWI	GGATC	6 cut(s)	527, 540, 637, 1323, 1799, 2047
AcoI	YGGCCR	2 cut(s)	262, 1562
AcsI	RAATTY	6 cut(s)	659, 859, 1666, 1744, 1858, 2239
AcyI	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
AfaI	GTAC	6 cut(s)	62, 204, 731, 1014, 1369, 1484
AfiI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
AhdI	GACNNNNNGTC	1 cut(s)	1272
AjiI	CACGTC	1 cut(s)	1274
AjnI	CCWGG	3 cut(s)	1655, 1733, 2293
AloI	GAACNNNNNNTCC	2 cut(s)	1959, 1991
AluBI	AGCT	8 cut(s)	284, 441, 557, 725, 1010, 1235, 1304, 2288
AluI	AGCT	8 cut(s)	284, 441, 557, 725, 1010, 1235, 1304, 2288
Alw21I	GWGCWC	1 cut(s)	1985
Alw26I	GTCTC	1 cut(s)	389
AlwI	GGATC	6 cut(s)	527, 540, 637, 1323, 1799, 2047
AlwNI	CAGNNNCTG	2 cut(s)	148, 1613
Ama87I	CYCGRG	1 cut(s)	129
Aor13HI	TCCGGA	1 cut(s)	2229
AoxI	GGCC	6 cut(s)	188, 250, 262, 1344, 1551, 1562
ApeKI	GCWGC	5 cut(s)	438, 749, 1598, 1898, 2288
ApoI	RAATTY	6 cut(s)	659, 859, 1666, 1744, 1858, 2239
ArsI	GACNNNNNNTTYG	2 cut(s)	851, 883
Asp700I	GAANNNNTTC	2 cut(s)	915, 2185
Asp718I	GGTACC	1 cut(s)	60
AspLEI	GCGC	1 cut(s)	1149
AspS9I	GGNCC	4 cut(s)	4, 189, 1345, 1903
AsuHPI	GGTGA	5 cut(s)	969, 1007, 1082, 1440, 2081
AvaI	CYCGRG	1 cut(s)	129
AvaII	GGWCC	2 cut(s)	4, 1903
BaeI	ACNNNNGTAYC	2 cut(s)	1593, 1626
BalI	TGGCCA	1 cut(s)	264
BamHI	GGATCC	1 cut(s)	532
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	2153
BbsI	GAAGAC	4 cut(s)	284, 1271, 2110, 2238
Bbv12I	GWGCWC	1 cut(s)	1985
BbvI	GCAGC	5 cut(s)	450, 736, 1585, 1885, 2275
BccI	CCATC	4 cut(s)	84, 358, 1344, 1538
BceAI	ACGGC	1 cut(s)	593
BciT130I	CCWGG	3 cut(s)	1657, 1735, 2295
BclI	TGATCA	1 cut(s)	2275
BcoDI	GTCTC	1 cut(s)	389
BfaI	CTAG	5 cut(s)	558, 776, 1452, 1989, 2114
BfmI	CTRYAG	2 cut(s)	549, 804
BfuAI	ACCTGC	1 cut(s)	2116
BglI	GCCNNNNNGGC	1 cut(s)	270
BisI	GCNGC	6 cut(s)	439, 750, 1562, 1599, 1899, 2289
BlsI	GCNGC	6 cut(s)	440, 751, 1563, 1600, 1900, 2290
Bme1390I	CCNGG	3 cut(s)	1657, 1735, 2295
Bme18I	GGWCC	2 cut(s)	4, 1903
BmeRI	GACNNNNNGTC	1 cut(s)	1272
BmeT110I	CYCGRG	1 cut(s)	129
BmgBI	CACGTC	1 cut(s)	1274
BmgT120I	GGNCC	4 cut(s)	4, 189, 1345, 1903
BmiI	GGNNCC	4 cut(s)	62, 348, 534, 1905
BmrFI	CCNGG	3 cut(s)	1657, 1735, 2295
BmrI	ACTGGG	1 cut(s)	2048
BmsI	GCATC	6 cut(s)	1576, 1642, 1720, 1858, 2067, 2197
BmuI	ACTGGG	1 cut(s)	2048
BpiI	GAAGAC	4 cut(s)	284, 1271, 2110, 2238
BplI	GAGNNNNNCTC	2 cut(s)	498, 530
BpuEI	CTTGAG	1 cut(s)	1155
BsaHI	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
BsaI	GGTCTC	1 cut(s)	389
BsaWI	WCCGGW	1 cut(s)	2229
BsaXI	ACNNNNNCTCC	8 cut(s)	151, 181, 477, 507, 1780, 1810, 1919, 1949
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
Bse1I	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 2054, 2081
Bse3DI	GCAATG	2 cut(s)	516, 882
BseAI	TCCGGA	1 cut(s)	2229
BseBI	CCWGG	3 cut(s)	1657, 1735, 2295
BseGI	GGATG	4 cut(s)	95, 1017, 1117, 2048
BseLI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
BseMI	GCAATG	2 cut(s)	516, 882
BseMII	CTCAG	1 cut(s)	395
BseNI	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 2054, 2081
BseXI	GCAGC	5 cut(s)	450, 736, 1585, 1885, 2275
BseYI	CCCAGC	1 cut(s)	716
BsgI	GTGCAG	3 cut(s)	1394, 1544, 2274
BshFI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1985
BsiHKCI	CYCGRG	1 cut(s)	129
BsiSI	CCGG	1 cut(s)	2230
BslFI	GGGAC	3 cut(s)	54, 107, 832
BslI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 795, 1342, 1560
BsmAI	GTCTC	1 cut(s)	389
BsmFI	GGGAC	3 cut(s)	54, 107, 832
BsnI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
Bso31I	GGTCTC	1 cut(s)	389
BsoBI	CYCGRG	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	1985, 2153
Bsp13I	TCCGGA	1 cut(s)	2229
Bsp1407I	TGTACA	1 cut(s)	1012
Bsp143I	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
Bsp19I	CCATGG	2 cut(s)	31, 288
BspACI	CCGC	4 cut(s)	892, 1418, 1561, 1901
BspANI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BspCNI	CTCAG	1 cut(s)	396
BspEI	TCCGGA	1 cut(s)	2229
BspHI	TCATGA	2 cut(s)	1239, 1276
BspLI	GGNNCC	4 cut(s)	62, 348, 534, 1905
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	1 cut(s)	2116
BspPI	GGATC	6 cut(s)	527, 540, 637, 1323, 1799, 2047
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	1 cut(s)	389
BsrDI	GCAATG	2 cut(s)	516, 882
BsrGI	TGTACA	1 cut(s)	1012
BsrI	ACTGG	7 cut(s)	16, 153, 536, 684, 1530, 2054, 2081
BssMI	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
Bst1107I	GTATAC	1 cut(s)	184
Bst2UI	CCWGG	3 cut(s)	1657, 1735, 2295
Bst4CI	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
Bst6I	CTCTTC	2 cut(s)	1192, 1275
BstACI	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
BstAPI	GCANNNNNTGC	2 cut(s)	1189, 2021
BstAUI	TGTACA	1 cut(s)	1012
BstDEI	CTNAG	3 cut(s)	404, 422, 1322
BstDSI	CCRYGG	2 cut(s)	31, 288
BstEII	GGTNACC	1 cut(s)	975
BstF5I	GGATG	4 cut(s)	95, 1017, 1117, 2048
BstHHI	GCGC	1 cut(s)	1149
BstKTI	GATC	9 cut(s)	535, 599, 645, 1003, 1245, 1318, 1807, 2042, 2278
BstMAI	GTCTC	1 cut(s)	389
BstMBI	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
BstMWI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 2021
BstNI	CCWGG	3 cut(s)	1657, 1735, 2295
BstNSI	RCATGY	2 cut(s)	965, 1147
BstPI	GGTNACC	1 cut(s)	975
BstSCI	CCNGG	3 cut(s)	1655, 1733, 2293
BstSFI	CTRYAG	2 cut(s)	549, 804
BstV1I	GCAGC	5 cut(s)	450, 736, 1585, 1885, 2275
BstV2I	GAAGAC	4 cut(s)	284, 1271, 2110, 2238
BstX2I	RGATCY	2 cut(s)	532, 1315
BstXI	CCANNNNNNTGG	2 cut(s)	260, 716
BstYI	RGATCY	2 cut(s)	532, 1315
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
BtgI	CCRYGG	2 cut(s)	31, 288
BtrI	CACGTC	1 cut(s)	1274
BtsCI	GGATG	4 cut(s)	95, 1017, 1117, 2048
BtsI	GCAGTG	3 cut(s)	114, 1689, 1767
BveI	ACCTGC	1 cut(s)	2116
CaiI	CAGNNNCTG	2 cut(s)	148, 1613
CciI	TCATGA	2 cut(s)	1239, 1276
CfoI	GCGC	1 cut(s)	1149
Cfr13I	GGNCC	4 cut(s)	4, 189, 1345, 1903
CseI	GACGC	5 cut(s)	349, 389, 1464, 1669, 1747
Csp6I	GTAC	6 cut(s)	61, 203, 730, 1013, 1368, 1483
CspCI	CAANNNNNGTGG	2 cut(s)	2017, 2052
CviQI	GTAC	6 cut(s)	61, 203, 730, 1013, 1368, 1483
DdeI	CTNAG	3 cut(s)	404, 422, 1322
DpnI	GATC	9 cut(s)	534, 598, 644, 1002, 1244, 1317, 1806, 2041, 2277
DpnII	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
DrdI	GACNNNNNNGTC	1 cut(s)	1580
DriI	GACNNNNNGTC	1 cut(s)	1272
DseDI	GACNNNNNNGTC	1 cut(s)	1580
EaeI	YGGCCR	2 cut(s)	262, 1562
Eam1104I	CTCTTC	2 cut(s)	1192, 1275
Eam1105I	GACNNNNNGTC	1 cut(s)	1272
EarI	CTCTTC	2 cut(s)	1192, 1275
Eco24I	GRGCYC	1 cut(s)	2153
Eco31I	GGTCTC	1 cut(s)	389
Eco32I	GATATC	2 cut(s)	215, 1911
Eco47I	GGWCC	2 cut(s)	4, 1903
Eco88I	CYCGRG	1 cut(s)	129
Eco91I	GGTNACC	1 cut(s)	975
EcoO65I	GGTNACC	1 cut(s)	975
EcoRII	CCWGG	3 cut(s)	1655, 1733, 2293
EcoRV	GATATC	2 cut(s)	215, 1911
EcoT38I	GRGCYC	1 cut(s)	2153
FaqI	GGGAC	3 cut(s)	54, 107, 832
FauI	CCCGC	1 cut(s)	899
FauNDI	CATATG	2 cut(s)	307, 377
FbaI	TGATCA	1 cut(s)	2275
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	6 cut(s)	439, 750, 1562, 1599, 1899, 2289
FokI	GGATG	4 cut(s)	102, 1004, 1124, 2035
FriOI	GRGCYC	1 cut(s)	2153
Fsp4HI	GCNGC	6 cut(s)	439, 750, 1562, 1599, 1899, 2289
FspBI	CTAG	5 cut(s)	558, 776, 1452, 1989, 2114
FspI	TGCGCA	1 cut(s)	1148
GlaI	GCGC	1 cut(s)	1148
GluI	GCNGC	6 cut(s)	439, 750, 1562, 1599, 1899, 2289
GsaI	CCCAGC	1 cut(s)	720
HaeIII	GGCC	6 cut(s)	190, 252, 264, 1346, 1553, 1564
HapII	CCGG	1 cut(s)	2230
HgaI	GACGC	5 cut(s)	349, 389, 1464, 1669, 1747
HhaI	GCGC	1 cut(s)	1149
Hin1I	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
Hin6I	GCGC	1 cut(s)	1147
HinP1I	GCGC	1 cut(s)	1147
HincII	GTYRAC	1 cut(s)	606
HindII	GTYRAC	1 cut(s)	606
HpaII	CCGG	1 cut(s)	2230
HphI	GGTGA	5 cut(s)	969, 1007, 1082, 1440, 2081
Hpy166II	GTNNAC	5 cut(s)	184, 606, 730, 1015, 1035
Hpy8I	GTNNAC	5 cut(s)	184, 606, 730, 1015, 1035
HpyAV	CCTTC	8 cut(s)	926, 1543, 1809, 2060, 2171, 2174, 2202, 2232
HpyCH4III	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
HpyCH4IV	ACGT	2 cut(s)	1215, 1273
HpyF10VI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 2021
HpyF3I	CTNAG	3 cut(s)	404, 422, 1322
HpySE526I	ACGT	2 cut(s)	1215, 1273
Hsp92I	GRCGYC	4 cut(s)	360, 1456, 1661, 1739
HspAI	GCGC	1 cut(s)	1147
Kpn2I	TCCGGA	1 cut(s)	2229
KpnI	GGTACC	1 cut(s)	64
Ksp22I	TGATCA	1 cut(s)	2275
Kzo9I	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
LmnI	GCTCC	2 cut(s)	498, 506
Lsp1109I	GCAGC	5 cut(s)	450, 736, 1585, 1885, 2275
LweI	GCATC	6 cut(s)	1576, 1642, 1720, 1858, 2067, 2197
MaeI	CTAG	5 cut(s)	558, 776, 1452, 1989, 2114
MaeII	ACGT	2 cut(s)	1215, 1273
MaeIII	GTNAC	8 cut(s)	241, 452, 466, 674, 975, 1103, 1216, 2083
MalI	GATC	9 cut(s)	534, 598, 644, 1002, 1244, 1317, 1806, 2041, 2277
MboI	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
MflI	RGATCY	2 cut(s)	532, 1315
MhlI	GDGCHC	2 cut(s)	1985, 2153
MlsI	TGGCCA	1 cut(s)	264
MluNI	TGGCCA	1 cut(s)	264
MlyI	GAGTC	8 cut(s)	141, 689, 826, 1335, 1415, 1568, 1610, 1883
MmeI	TCCRAC	1 cut(s)	670
Mox20I	TGGCCA	1 cut(s)	264
MroI	TCCGGA	1 cut(s)	2229
MroXI	GAANNNNTTC	2 cut(s)	915, 2185
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	3 cut(s)	75, 225, 570
MslI	CAYNNNNRTG	2 cut(s)	1110, 2205
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	2 cut(s)	441, 2288
MspI	CCGG	1 cut(s)	2230
MspR9I	CCNGG	3 cut(s)	1657, 1735, 2295
MvaI	CCWGG	3 cut(s)	1657, 1735, 2295
MwoI	GCNNNNNNNGC	6 cut(s)	270, 722, 1189, 1352, 1595, 2021
NcoI	CCATGG	2 cut(s)	31, 288
NdeI	CATATG	2 cut(s)	307, 377
NdeII	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
NlaIV	GGNNCC	4 cut(s)	62, 348, 534, 1905
NmuCI	GTSAC	2 cut(s)	975, 1103
NsbI	TGCGCA	1 cut(s)	1148
NspI	RCATGY	2 cut(s)	965, 1147
PaeR7I	CTCGAG	1 cut(s)	129
PagI	TCATGA	2 cut(s)	1239, 1276
PdmI	GAANNNNTTC	2 cut(s)	915, 2185
PfeI	GAWTC	6 cut(s)	52, 152, 880, 916, 1165, 1883
PflMI	CCANNNNNTGG	2 cut(s)	37, 356
PkrI	GCNGC	6 cut(s)	440, 751, 1563, 1600, 1900, 2290
PleI	GAGTC	8 cut(s)	140, 688, 826, 1334, 1415, 1568, 1610, 1883
PpsI	GAGTC	8 cut(s)	140, 688, 826, 1334, 1415, 1568, 1610, 1883
PsiI	TTATAA	1 cut(s)	593
Psp1406I	AACGTT	1 cut(s)	1215
Psp6I	CCWGG	3 cut(s)	1655, 1733, 2293
PspEI	GGTNACC	1 cut(s)	975
PspFI	CCCAGC	1 cut(s)	716
PspGI	CCWGG	3 cut(s)	1655, 1733, 2293
PspN4I	GGNNCC	4 cut(s)	62, 348, 534, 1905
PspPI	GGNCC	4 cut(s)	4, 189, 1345, 1903
PstI	CTGCAG	1 cut(s)	808
PstNI	CAGNNNCTG	2 cut(s)	148, 1613
PsuI	RGATCY	2 cut(s)	532, 1315
PvuII	CAGCTG	2 cut(s)	441, 2288
RsaI	GTAC	6 cut(s)	62, 204, 731, 1014, 1369, 1484
RsaNI	GTAC	6 cut(s)	61, 203, 730, 1013, 1368, 1483
RseI	CAYNNNNRTG	2 cut(s)	1110, 2205
SaqAI	TTAA	3 cut(s)	75, 225, 570
SatI	GCNGC	6 cut(s)	439, 750, 1562, 1599, 1899, 2289
Sau3AI	GATC	9 cut(s)	532, 596, 642, 1000, 1242, 1315, 1804, 2039, 2275
Sau96I	GGNCC	4 cut(s)	4, 189, 1345, 1903
SchI	GAGTC	8 cut(s)	141, 689, 826, 1335, 1415, 1568, 1610, 1883
ScrFI	CCNGG	3 cut(s)	1657, 1735, 2295
SduI	GDGCHC	2 cut(s)	1985, 2153
SfaNI	GCATC	6 cut(s)	1576, 1642, 1720, 1858, 2067, 2197
SfcI	CTRYAG	2 cut(s)	549, 804
Sfr274I	CTCGAG	1 cut(s)	129
SinI	GGWCC	2 cut(s)	4, 1903
SlaI	CTCGAG	1 cut(s)	129
SmiMI	CAYNNNNRTG	2 cut(s)	1110, 2205
SmlI	CTYRAG	2 cut(s)	129, 1170
SmoI	CTYRAG	2 cut(s)	129, 1170
SsiI	CCGC	4 cut(s)	892, 1418, 1561, 1901
SspMI	CTAG	5 cut(s)	558, 776, 1452, 1989, 2114
StyD4I	CCNGG	3 cut(s)	1655, 1733, 2293
TaaI	ACNGT	9 cut(s)	72, 202, 610, 823, 955, 1039, 1372, 1406, 1558
TaiI	ACGT	2 cut(s)	1218, 1276
TaqI	TCGA	3 cut(s)	130, 1584, 1844
TatI	WGTACW	2 cut(s)	729, 1012
TauI	GCSGC	1 cut(s)	1564
TfiI	GAWTC	6 cut(s)	52, 152, 880, 916, 1165, 1883
Tru1I	TTAA	3 cut(s)	75, 225, 570
Tru9I	TTAA	3 cut(s)	75, 225, 570
TseFI	GTSAC	2 cut(s)	975, 1103
TseI	GCWGC	5 cut(s)	438, 749, 1598, 1898, 2288
Tsp45I	GTSAC	2 cut(s)	975, 1103
TspDTI	ATGAA	7 cut(s)	225, 754, 872, 924, 954, 1293, 2184
TspGWI	ACGGA	2 cut(s)	1355, 1940
Van91I	CCANNNNNTGG	2 cut(s)	37, 356
VpaK11BI	GGWCC	2 cut(s)	4, 1903
XapI	RAATTY	6 cut(s)	659, 859, 1666, 1744, 1858, 2239
XbaI	TCTAGA	1 cut(s)	2113
XceI	RCATGY	2 cut(s)	965, 1147
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1105
XhoI	CTCGAG	1 cut(s)	129
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	915, 2185
XspI	CTAG	5 cut(s)	558, 776, 1452, 1989, 2114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.