Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Reverse (-)

69622546 .. 69629976

Gene Structure 7431 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00132610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2025 bp

ATGGTCCTCACTGGTTCGTTGCAATTATCCCATGGATTGGGACTTTGCAAGAATCAGGGGTACCAGAAACAGTTTAAGAGGGGAATAGGATGGGACAAATTACATCTATCAAGCACTGCCCTTACATCTCGAGTCTCGTTTCAGAAACTGGATTCTTGGAGTATTGGTTTATCAGAGAATGTATACAGGCCCATACATACTGTACCTCACAGATATCATGTGCTTAAGTGCCATTCATCTGTAACATCAGGCCAATCTTTTGGCCAACATGGCTTGAAGACAGCTACCATGGTGTTGAAAAGGTCATATGATGTTTTGCAAGGTTATCCTCTTATATTCAAATTGGTTCCATCAGTTGGCGTCATTATATTTGCCATATGGGGTCTCGCACCACTTATGCGTCTGAGCAGAAACCTAATCCTTAGTAAGAATGATAGCAGCTGGAAAAAGAGTAACACACACTATGTTACGAACTCATATGTTCAACCTTTGCTCCTATGGACGGGAGCAATGCTCATTTGCAGGGCATTGGATCCAGTTGTTCTACCTACAGAAGCTAGTCAGGTCGTTAAGCAACGGCTTTTGGACTTTATAAGATCGCTGTCAACTGTTCTGTCTTTTGCCTATTGTTTATCAAGTACTCTTCAATGCAATGAAATTAACACTTCTCTAATTCTTTTACGCAGTTTGATCCAACAAACACAGAAATTCTTTATGGAGAGTAACGAGTCCAGTGATACAAGAAATATGGGTTTCCAATTTGCTGGGAAAGCTGTGTACTCTGCTGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAAATGGCTTACTGCAGGAGGTCTTGGGACAGTCTTATTGACTCTGGCTGGCCGTGAGATATTTACAAATTTCCTTTCAAGTGCAATGATTCATGCCACCCGCCCCTTTGTTGTGAATGAATGGATTCAAACTAAAATAGAAGGCTATGAAGTATCTGGCACAGTTGAGAGTATTGTTGCCGACATGCGCAAAGTCTTGTCCAAGAATCCTCAAGTGGAACAGCAGAGGTTGCATAGAAGAGTATTTCTGGAAAACGTTACTCCCGATAATCAAGCTCTCATGATCTTGATTTCCTGTTTTGTGAAGACATCACATCATGAAGAGTATCTCTGTGTCAAGGAAGCTATACTCATGGATCTTCTTAGAGTCATCAGCCATCACAAGGCCCGACTTGCCACACCAATCCGTACAGTGCAGAAAATGTATAGCGATACCGACTTGGACAGTATGCCATTTGCGGACTCAATGTATAGTCGTGGTGGAACCAGTGGAGAGCGAGATGGGAAGGCCACAGTGCGGCCACAACCTGACTCCAAGGTGGATGCTAAAGTTGCTGCAACATCAGTATCTGACTCCAAGACAACACCAACATCTGAAACCAAGGGAGATGCCAGGACGCCAAATTCAATCCCCAAGGACGACCCCACTGCTTCAAAGTCATCTCCTTCTGATCCCAAAGTAGTTGATAAAGCAACAATCAAGTCAAGGTCGAACTCCAATACAAATTCTAAAGATGCAGAAAAGTCTGATTCTGACTCCAAGGCTGCGGACCCGATATCTAATCATTTGACGGAGAAGATGTCTGGCAGAAAGCAACTGAATAATGCAAGTGGAAATGTAGTTCAGAGCACTTCTAGTAATCCTGCATTTTCCTCATCTGCAAGTGGTGCTGACAAAACGGGTGGATCAATGACATCCCCAGTGAAACAAGAAGGTGAAAAGATGCCAGTTGCTGAGCCACCAACAACAAGACCTGCTCTAGAAGAAAATATAGTCCTTGGTGTTGCCTTGGAGGGCTCAAAGAGAACCCTTCCTATTGAGGAAGGAATGGCTTCCCCTTCATCTCACGCTGATGCAAACGATTTGCCTTCTTTCCGGAAAGGAAATTCGTCTTCAAATACTGATAAGGGTAAGAAAAATGATCAAGTTCCAGCTGCACCAGGCACAAGTTCTGATAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

674

Amino Acids

73.61

Weight (kDa)

9.23

Isoelectric Point (pI)

42.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	153 - 298	1.5e-63	Mechanosensitive channel protein 2/3 domain
Msl2-3_C	PF24956	333 - 405	1.3e-35	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	593
Acc16I	TGCGCA	1 cut(s)	1028
Acc36I	ACCTGC	1 cut(s)	1822
Acc65I	GGTACC	1 cut(s)	60
AccB1I	GGYRCC	1 cut(s)	60
AccB7I	CCANNNNNTGG	2 cut(s)	37, 356
AccI	GTMKAC	1 cut(s)	183
AccIII	TCCGGA	1 cut(s)	1935
AciI	CCGC	4 cut(s)	940, 1298, 1357, 1607
AclI	AACGTT	1 cut(s)	1095
AclWI	GGATC	6 cut(s)	527, 540, 685, 1203, 1505, 1753
AcoI	YGGCCR	3 cut(s)	262, 889, 1358
AcsI	RAATTY	5 cut(s)	707, 907, 1462, 1564, 1945
AcyI	GRCGYC	2 cut(s)	360, 1457
AfaI	GTAC	5 cut(s)	62, 204, 640, 779, 1249
AfiI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 843, 1222, 1356
AflII	CTTAAG	1 cut(s)	224
AjnI	CCWGG	2 cut(s)	1451, 1999
AloI	GAACNNNNNNTCC	2 cut(s)	1665, 1697
AluBI	AGCT	7 cut(s)	284, 441, 557, 773, 1115, 1184, 1994
AluI	AGCT	7 cut(s)	284, 441, 557, 773, 1115, 1184, 1994
Alw21I	GWGCWC	1 cut(s)	1691
Alw26I	GTCTC	2 cut(s)	139, 389
AlwI	GGATC	6 cut(s)	527, 540, 685, 1203, 1505, 1753
AlwNI	CAGNNNCTG	3 cut(s)	148, 1409, 1793
Ama87I	CYCGRG	1 cut(s)	129
Aor13HI	TCCGGA	1 cut(s)	1935
AoxI	GGCC	7 cut(s)	188, 250, 262, 889, 1224, 1347, 1358
ApeKI	GCWGC	5 cut(s)	438, 797, 1394, 1604, 1994
ApoI	RAATTY	5 cut(s)	707, 907, 1462, 1564, 1945
Asp700I	GAANNNNTTC	2 cut(s)	963, 1891
Asp718I	GGTACC	1 cut(s)	60
AspLEI	GCGC	1 cut(s)	1029
AspS9I	GGNCC	4 cut(s)	4, 189, 1225, 1609
AsuHPI	GGTGA	1 cut(s)	1787
AvaI	CYCGRG	1 cut(s)	129
AvaII	GGWCC	2 cut(s)	4, 1609
BaeI	ACNNNNGTAYC	2 cut(s)	1389, 1422
BalI	TGGCCA	1 cut(s)	264
BamHI	GGATCC	1 cut(s)	532
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	1859
BbsI	GAAGAC	3 cut(s)	284, 1151, 1944
Bbv12I	GWGCWC	1 cut(s)	1691
BbvI	GCAGC	5 cut(s)	450, 784, 1381, 1591, 1981
BccI	CCATC	4 cut(s)	84, 358, 1224, 1334
BceAI	ACGGC	2 cut(s)	593, 876
BcgI	CGANNNNNNTGC	2 cut(s)	1469, 1503
BciT130I	CCWGG	2 cut(s)	1453, 2001
BclI	TGATCA	1 cut(s)	1981
BcoDI	GTCTC	2 cut(s)	139, 389
BfaI	CTAG	4 cut(s)	558, 824, 1695, 1820
BfmI	CTRYAG	2 cut(s)	549, 852
BfrI	CTTAAG	1 cut(s)	224
BfuAI	ACCTGC	1 cut(s)	1822
BglI	GCCNNNNNGGC	1 cut(s)	270
BisI	GCNGC	6 cut(s)	439, 798, 1358, 1395, 1605, 1995
BlpI	GCTNAGC	1 cut(s)	1794
BlsI	GCNGC	6 cut(s)	440, 799, 1359, 1396, 1606, 1996
BmcAI	AGTACT	1 cut(s)	640
Bme1390I	CCNGG	2 cut(s)	1453, 2001
Bme18I	GGWCC	2 cut(s)	4, 1609
BmeT110I	CYCGRG	1 cut(s)	129
BmgT120I	GGNCC	4 cut(s)	4, 189, 1225, 1609
BmiI	GGNNCC	5 cut(s)	62, 348, 534, 1324, 1611
BmrFI	CCNGG	2 cut(s)	1453, 2001
BmrI	ACTGGG	1 cut(s)	1754
BmsI	GCATC	5 cut(s)	1372, 1438, 1564, 1773, 1903
BmuI	ACTGGG	1 cut(s)	1754
BpiI	GAAGAC	3 cut(s)	284, 1151, 1944
BplI	GAGNNNNNCTC	2 cut(s)	498, 530
Bpu1102I	GCTNAGC	1 cut(s)	1794
BpuEI	CTTGAG	1 cut(s)	1035
BsaHI	GRCGYC	2 cut(s)	360, 1457
BsaI	GGTCTC	1 cut(s)	389
BsaJI	CCNNGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
BsaWI	WCCGGW	1 cut(s)	1935
BsaXI	ACNNNNNCTCC	6 cut(s)	151, 181, 477, 507, 1625, 1655
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 843, 1222, 1356
Bse1I	ACTGG	7 cut(s)	16, 153, 536, 732, 1326, 1760, 1787
Bse3DI	GCAATG	3 cut(s)	516, 658, 930
BseAI	TCCGGA	1 cut(s)	1935
BseBI	CCWGG	2 cut(s)	1453, 2001
BseDI	CCNNGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
BseGI	GGATG	3 cut(s)	95, 1387, 1754
BseLI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 843, 1222, 1356
BseMI	GCAATG	3 cut(s)	516, 658, 930
BseMII	CTCAG	2 cut(s)	395, 1785
BseNI	ACTGG	7 cut(s)	16, 153, 536, 732, 1326, 1760, 1787
BseXI	GCAGC	5 cut(s)	450, 784, 1381, 1591, 1981
BseYI	CCCAGC	1 cut(s)	764
BsgI	GTGCAG	2 cut(s)	1274, 1980
BshFI	GGCC	7 cut(s)	190, 252, 264, 891, 1226, 1349, 1360
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1691
BsiHKCI	CYCGRG	1 cut(s)	129
BsiSI	CCGG	1 cut(s)	1936
BslFI	GGGAC	3 cut(s)	54, 107, 880
BslI	CCNNNNNNNGG	6 cut(s)	37, 356, 502, 843, 1222, 1356
BsmAI	GTCTC	2 cut(s)	139, 389
BsmFI	GGGAC	3 cut(s)	54, 107, 880
BsnI	GGCC	7 cut(s)	190, 252, 264, 891, 1226, 1349, 1360
Bso31I	GGTCTC	1 cut(s)	389
BsoBI	CYCGRG	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	1691, 1859
Bsp13I	TCCGGA	1 cut(s)	1935
Bsp143I	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
Bsp1720I	GCTNAGC	1 cut(s)	1794
Bsp19I	CCATGG	2 cut(s)	31, 288
BspACI	CCGC	4 cut(s)	940, 1298, 1357, 1607
BspANI	GGCC	7 cut(s)	190, 252, 264, 891, 1226, 1349, 1360
BspCNI	CTCAG	2 cut(s)	396, 1786
BspEI	TCCGGA	1 cut(s)	1935
BspHI	TCATGA	2 cut(s)	1119, 1156
BspLI	GGNNCC	5 cut(s)	62, 348, 534, 1324, 1611
BspMAI	CTGCAG	1 cut(s)	856
BspMI	ACCTGC	1 cut(s)	1822
BspPI	GGATC	6 cut(s)	527, 540, 685, 1203, 1505, 1753
BspT107I	GGYRCC	1 cut(s)	60
BspTI	CTTAAG	1 cut(s)	224
BspTNI	GGTCTC	1 cut(s)	389
BsrDI	GCAATG	3 cut(s)	516, 658, 930
BsrI	ACTGG	7 cut(s)	16, 153, 536, 732, 1326, 1760, 1787
BssECI	CCNNGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
BssMI	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	2 cut(s)	360, 1457
BssT1I	CCWWGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
Bst1107I	GTATAC	1 cut(s)	184
Bst2UI	CCWGG	2 cut(s)	1453, 2001
Bst4CI	ACNGT	8 cut(s)	72, 202, 610, 871, 1003, 1252, 1286, 1354
Bst6I	CTCTTC	3 cut(s)	648, 1072, 1155
BstACI	GRCGYC	2 cut(s)	360, 1457
BstAFI	CTTAAG	1 cut(s)	224
BstAPI	GCANNNNNTGC	2 cut(s)	1069, 1727
BstC8I	GCNNGC	1 cut(s)	889
BstDEI	CTNAG	4 cut(s)	404, 422, 1202, 1794
BstDSI	CCRYGG	2 cut(s)	31, 288
BstF5I	GGATG	3 cut(s)	95, 1387, 1754
BstHHI	GCGC	1 cut(s)	1029
BstKTI	GATC	8 cut(s)	535, 599, 693, 1125, 1198, 1513, 1748, 1984
BstMAI	GTCTC	2 cut(s)	139, 389
BstMBI	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
BstMWI	GCNNNNNNNGC	6 cut(s)	270, 770, 1069, 1232, 1391, 1727
BstNI	CCWGG	2 cut(s)	1453, 2001
BstNSI	RCATGY	1 cut(s)	1027
BstSCI	CCNGG	2 cut(s)	1451, 1999
BstSFI	CTRYAG	2 cut(s)	549, 852
BstV1I	GCAGC	5 cut(s)	450, 784, 1381, 1591, 1981
BstV2I	GAAGAC	3 cut(s)	284, 1151, 1944
BstX2I	RGATCY	2 cut(s)	532, 1195
BstXI	CCANNNNNNTGG	2 cut(s)	260, 764
BstYI	RGATCY	2 cut(s)	532, 1195
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	7 cut(s)	190, 252, 264, 891, 1226, 1349, 1360
BtgI	CCRYGG	2 cut(s)	31, 288
BtsCI	GGATG	3 cut(s)	95, 1387, 1754
BtsI	GCAGTG	2 cut(s)	114, 1485
BtsIMutI	CAGTG	8 cut(s)	9, 114, 739, 1257, 1333, 1359, 1485, 1767
BveI	ACCTGC	1 cut(s)	1822
Cac8I	GCNNGC	1 cut(s)	889
CaiI	CAGNNNCTG	3 cut(s)	148, 1409, 1793
CciI	TCATGA	2 cut(s)	1119, 1156
CfoI	GCGC	1 cut(s)	1029
Cfr13I	GGNCC	4 cut(s)	4, 189, 1225, 1609
CseI	GACGC	3 cut(s)	349, 389, 1465
Csp6I	GTAC	5 cut(s)	61, 203, 639, 778, 1248
CspCI	CAANNNNNGTGG	2 cut(s)	1723, 1758
CviQI	GTAC	5 cut(s)	61, 203, 639, 778, 1248
DdeI	CTNAG	4 cut(s)	404, 422, 1202, 1794
DpnI	GATC	8 cut(s)	534, 598, 692, 1124, 1197, 1512, 1747, 1983
DpnII	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
EaeI	YGGCCR	3 cut(s)	262, 889, 1358
Eam1104I	CTCTTC	3 cut(s)	648, 1072, 1155
EarI	CTCTTC	3 cut(s)	648, 1072, 1155
Eco130I	CCWWGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
Eco24I	GRGCYC	1 cut(s)	1859
Eco31I	GGTCTC	1 cut(s)	389
Eco32I	GATATC	2 cut(s)	215, 1617
Eco47I	GGWCC	2 cut(s)	4, 1609
Eco88I	CYCGRG	1 cut(s)	129
EcoRII	CCWGG	2 cut(s)	1451, 1999
EcoRV	GATATC	2 cut(s)	215, 1617
EcoT14I	CCWWGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
EcoT38I	GRGCYC	1 cut(s)	1859
ErhI	CCWWGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
FaqI	GGGAC	3 cut(s)	54, 107, 880
FauI	CCCGC	1 cut(s)	947
FauNDI	CATATG	3 cut(s)	307, 377, 478
FbaI	TGATCA	1 cut(s)	1981
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	6 cut(s)	439, 798, 1358, 1395, 1605, 1995
FokI	GGATG	3 cut(s)	102, 1394, 1741
FriOI	GRGCYC	1 cut(s)	1859
Fsp4HI	GCNGC	6 cut(s)	439, 798, 1358, 1395, 1605, 1995
FspBI	CTAG	4 cut(s)	558, 824, 1695, 1820
FspI	TGCGCA	1 cut(s)	1028
GlaI	GCGC	1 cut(s)	1028
GluI	GCNGC	6 cut(s)	439, 798, 1358, 1395, 1605, 1995
GsaI	CCCAGC	1 cut(s)	768
HaeIII	GGCC	7 cut(s)	190, 252, 264, 891, 1226, 1349, 1360
HapII	CCGG	1 cut(s)	1936
HgaI	GACGC	3 cut(s)	349, 389, 1465
HhaI	GCGC	1 cut(s)	1029
Hin1I	GRCGYC	2 cut(s)	360, 1457
Hin6I	GCGC	1 cut(s)	1027
HinP1I	GCGC	1 cut(s)	1027
HincII	GTYRAC	1 cut(s)	606
HindII	GTYRAC	1 cut(s)	606
HpaII	CCGG	1 cut(s)	1936
HphI	GGTGA	1 cut(s)	1787
Hpy166II	GTNNAC	3 cut(s)	184, 606, 778
Hpy188III	TCNNGA	8 cut(s)	129, 1088, 1103, 1120, 1126, 1157, 1820, 1936
Hpy8I	GTNNAC	3 cut(s)	184, 606, 778
HpyAV	CCTTC	8 cut(s)	974, 1339, 1515, 1766, 1877, 1880, 1908, 1938
HpyCH4III	ACNGT	8 cut(s)	72, 202, 610, 871, 1003, 1252, 1286, 1354
HpyCH4IV	ACGT	1 cut(s)	1095
HpyF10VI	GCNNNNNNNGC	6 cut(s)	270, 770, 1069, 1232, 1391, 1727
HpyF3I	CTNAG	4 cut(s)	404, 422, 1202, 1794
HpySE526I	ACGT	1 cut(s)	1095
Hsp92I	GRCGYC	2 cut(s)	360, 1457
HspAI	GCGC	1 cut(s)	1027
Kpn2I	TCCGGA	1 cut(s)	1935
KpnI	GGTACC	1 cut(s)	64
Ksp22I	TGATCA	1 cut(s)	1981
Kzo9I	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
LmnI	GCTCC	2 cut(s)	498, 506
Lsp1109I	GCAGC	5 cut(s)	450, 784, 1381, 1591, 1981
LweI	GCATC	5 cut(s)	1372, 1438, 1564, 1773, 1903
MaeI	CTAG	4 cut(s)	558, 824, 1695, 1820
MaeII	ACGT	1 cut(s)	1095
MaeIII	GTNAC	5 cut(s)	241, 452, 466, 722, 1096
MalI	GATC	8 cut(s)	534, 598, 692, 1124, 1197, 1512, 1747, 1983
MboI	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
MboII	GAAGA	9 cut(s)	289, 635, 1089, 1156, 1172, 1190, 1648, 1835, 1944
MflI	RGATCY	2 cut(s)	532, 1195
MhlI	GDGCHC	2 cut(s)	1691, 1859
MlsI	TGGCCA	1 cut(s)	264
MluNI	TGGCCA	1 cut(s)	264
MlyI	GAGTC	8 cut(s)	141, 737, 874, 1215, 1295, 1364, 1406, 1589
MmeI	TCCRAC	1 cut(s)	718
Mox20I	TGGCCA	1 cut(s)	264
MroI	TCCGGA	1 cut(s)	1935
MroXI	GAANNNNTTC	2 cut(s)	963, 1891
MscI	TGGCCA	1 cut(s)	264
MseI	TTAA	4 cut(s)	75, 225, 570, 660
MslI	CAYNNNNRTG	1 cut(s)	1911
Msp20I	TGGCCA	1 cut(s)	264
MspA1I	CMGCKG	2 cut(s)	441, 1994
MspCI	CTTAAG	1 cut(s)	224
MspI	CCGG	1 cut(s)	1936
MspR9I	CCNGG	2 cut(s)	1453, 2001
MvaI	CCWGG	2 cut(s)	1453, 2001
MwoI	GCNNNNNNNGC	6 cut(s)	270, 770, 1069, 1232, 1391, 1727
NcoI	CCATGG	2 cut(s)	31, 288
NdeI	CATATG	3 cut(s)	307, 377, 478
NdeII	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
NlaIV	GGNNCC	5 cut(s)	62, 348, 534, 1324, 1611
NsbI	TGCGCA	1 cut(s)	1028
NspI	RCATGY	1 cut(s)	1027
PaeR7I	CTCGAG	1 cut(s)	129
PagI	TCATGA	2 cut(s)	1119, 1156
PcsI	WCGNNNNNNNCGW	1 cut(s)	1101
PdmI	GAANNNNTTC	2 cut(s)	963, 1891
PfeI	GAWTC	6 cut(s)	52, 152, 928, 964, 1045, 1589
PflMI	CCANNNNNTGG	2 cut(s)	37, 356
PkrI	GCNGC	6 cut(s)	440, 799, 1359, 1396, 1606, 1996
PleI	GAGTC	8 cut(s)	140, 736, 874, 1214, 1295, 1364, 1406, 1589
PpsI	GAGTC	8 cut(s)	140, 736, 874, 1214, 1295, 1364, 1406, 1589
PsiI	TTATAA	1 cut(s)	593
Psp1406I	AACGTT	1 cut(s)	1095
Psp6I	CCWGG	2 cut(s)	1451, 1999
PspFI	CCCAGC	1 cut(s)	764
PspGI	CCWGG	2 cut(s)	1451, 1999
PspN4I	GGNNCC	5 cut(s)	62, 348, 534, 1324, 1611
PspPI	GGNCC	4 cut(s)	4, 189, 1225, 1609
PstI	CTGCAG	1 cut(s)	856
PstNI	CAGNNNCTG	3 cut(s)	148, 1409, 1793
PsuI	RGATCY	2 cut(s)	532, 1195
PvuII	CAGCTG	2 cut(s)	441, 1994
RsaI	GTAC	5 cut(s)	62, 204, 640, 779, 1249
RsaNI	GTAC	5 cut(s)	61, 203, 639, 778, 1248
RseI	CAYNNNNRTG	1 cut(s)	1911
SaqAI	TTAA	4 cut(s)	75, 225, 570, 660
SatI	GCNGC	6 cut(s)	439, 798, 1358, 1395, 1605, 1995
Sau3AI	GATC	8 cut(s)	532, 596, 690, 1122, 1195, 1510, 1745, 1981
Sau96I	GGNCC	4 cut(s)	4, 189, 1225, 1609
ScaI	AGTACT	1 cut(s)	640
SchI	GAGTC	8 cut(s)	141, 737, 874, 1215, 1295, 1364, 1406, 1589
ScrFI	CCNGG	2 cut(s)	1453, 2001
SduI	GDGCHC	2 cut(s)	1691, 1859
SfaNI	GCATC	5 cut(s)	1372, 1438, 1564, 1773, 1903
SfcI	CTRYAG	2 cut(s)	549, 852
Sfr274I	CTCGAG	1 cut(s)	129
SinI	GGWCC	2 cut(s)	4, 1609
SlaI	CTCGAG	1 cut(s)	129
SmiMI	CAYNNNNRTG	1 cut(s)	1911
SmlI	CTYRAG	3 cut(s)	129, 224, 1050
SmoI	CTYRAG	3 cut(s)	129, 224, 1050
SsiI	CCGC	4 cut(s)	940, 1298, 1357, 1607
SspMI	CTAG	4 cut(s)	558, 824, 1695, 1820
StyD4I	CCNGG	2 cut(s)	1451, 1999
StyI	CCWWGG	8 cut(s)	31, 288, 1374, 1440, 1473, 1599, 1837, 1848
TaaI	ACNGT	8 cut(s)	72, 202, 610, 871, 1003, 1252, 1286, 1354
TaiI	ACGT	1 cut(s)	1098
TaqI	TCGA	2 cut(s)	130, 1550
TatI	WGTACW	2 cut(s)	638, 777
TauI	GCSGC	1 cut(s)	1360
TfiI	GAWTC	6 cut(s)	52, 152, 928, 964, 1045, 1589
Tru1I	TTAA	4 cut(s)	75, 225, 570, 660
Tru9I	TTAA	4 cut(s)	75, 225, 570, 660
TscAI	CASTG	8 cut(s)	16, 121, 739, 1257, 1333, 1359, 1492, 1767
TseI	GCWGC	5 cut(s)	438, 797, 1394, 1604, 1994
TspDTI	ATGAA	8 cut(s)	225, 669, 802, 920, 972, 1002, 1173, 1890
TspGWI	ACGGA	2 cut(s)	1235, 1646
TspRI	CASTG	8 cut(s)	16, 121, 739, 1257, 1333, 1359, 1492, 1767
Van91I	CCANNNNNTGG	2 cut(s)	37, 356
Vha464I	CTTAAG	1 cut(s)	224
VpaK11BI	GGWCC	2 cut(s)	4, 1609
XapI	RAATTY	5 cut(s)	707, 907, 1462, 1564, 1945
XbaI	TCTAGA	1 cut(s)	1819
XceI	RCATGY	1 cut(s)	1027
XhoI	CTCGAG	1 cut(s)	129
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	963, 1891
XspI	CTAG	4 cut(s)	558, 824, 1695, 1820
ZrmI	AGTACT	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.