Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005595

Physical Location & Seq

Genomic Coordinates Forward (+)

31480500 .. 31495527

Gene Structure 15028 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1312300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGGTTCTTGCTGGTTCATTGCAATTGTCCCATGGATTGGGACTTTGCAAGAATCAGAGGTACAGAGAACAATTTAAGTGTGGAATAGAAAGGGACAAGTTACATTCCTTAAACACCGGCCTTACATCAAGATTTGTGTTTCAGAAGCAGGATTCTTGGAGTATGAGTTTGTCAGACAATGTATACAGGCCCTTACATACTGTATCTCACAGATATCAAGCATTTAAGTGCCATTCCGTGCTAACATCAGGCCAACATGGCTTGAAGACAGCTGCGATGGCGTTGACAAGATCATATAATGCTTTGCAAGGTTGTCCTCTTGTATTCAAATTGGTTCCATCAGTTGGCCTCATTATTTTTTCCGTATGGGGTCTTCCACCACTTATGCGCCTGATCAGAAATCTGGTCCTTCAAAAGAGTGATAGTAGTTGGAAAAAGAGCAATACGCATTACGTGACAACCTCATATATTCAACCTTTGCTGCTTTGGACAGGAGCAATTCTCATTTGCAGGGCATTGGATCCAGTCGTTCTATCTACAGAAGCTAGCCAGGCTGTTAAGAAACGGCTTTTGGATTTTATAAGATCATTGTCAACTGTTCTGGCATTTGCCTATTGTTTATCAAGTGCGATTCAACAAGCACAGAAATTTTTTATGGAGAGTAGTGACTCCAGTACTACAAGAAATATGGGTTTTCAATTTGCCGGGAAGGCTGTTTACTCTGCAGTATGGGTTGCTGCTGTTTCGTTATTCATGGAGTTGCTAGGTTTCTCTACCCAAAGGTGGCTTACTGCAGGAGGTCTTGGGACAGTACTATTGACTCTGGCTGGTCGTGAGATCTTCACCAATTTCCTGTCAAGTGTAATGATTCATGCGACTCGCCCTTTTGTTGCAAATGAATGGATTCAAACAAAAATTGAAGGCTATGATGTATCTGGAACTGTTGAGCATGTGGGTTGGTGGTCACCGACAATTATAAGAGGTGAAGATCGTGAAGCAGTTCACATTCCAAACCACCAGTTCACAGTGAATGTTGTGAGAAATCTCAGTCAAAAAACTCATTGGCGTATCAAAACTCACCTTGCCATCAGCCATTTGGATGTCCATAAGATAAATAATATTGTTGCAGACATGCGCAAAGTCTTGTCCAAGAACCCTCAAGTTGAGCAGCAGAGGTTGCATAGAAGAGTATTTCTGGATAATGTTACCCCTGAAAACCAAGCTCTTTTGATCTTGATTTCCTGTTTTGTGAAGACGTCACATTATGAAGAGTATCTGTGCGTTAAGGAAGCTATAATGTTGGATCTTCTTAGAGTCATTAGCCATCACAAGGCCCGACTTGCCACACCAATTCGTACGGTTCAGAAAATGTATAGTGATCCCGATTTGGACAGTGTGCCATTTGCAGATTCAATGTATAACCATGCTGGAGCAAGATCCAGACGCCCATTCATACTGATTGAACCCTCATACAAAATCAATGGAGAAGATAAAAAAAGGAGTCGTACGCAAACCAGTGGAGAGCGAGATGGGAAGGCCACAATGCGGCCATCTCCTGACTCCAAGGCCAGAGAAACACCAACTTCTGACACCAAGTCAGATTCTAAAGCCGGAGCAACATCAGTATCTGACTCCAAGACCAGAGAAACACCAACATCTGATACCACAGCAGGTGCCAAGAGTGGAGAAACGCCAAATTCAAACACAAAGGAGGACCCGAAGGGTGCAAAATCATCTACATCTAATCCCAAAGTTAGTGACAAAGAAACAGTGAAGTCAACACTTAATTCGGTCTCCAAGTCAAATTCTAGAGACACTGAAAAGTCTGATTCTGACTCCAGAGCAACGGGCATAGCATCTAATAATTCGGTGCAGAACTCTGACAGAAAGCAACAAAAGACTGCAAGTGGATCAACGACTTCTCCAGTGAAACAGGAAGGTGAAAGGACGCCTGTTCCCGAGCCACCAATATCAAGGCCTGCTATAGAAGAAAACATAGTGCTTGGTGTTGCTTTAGAGGGCTCAAAGAGAACCCTTCCGATTGAGGAGGCGATGGCTTCCCCTTCGGCTCATGCAGAGGCAAACGATTTGGCTCCTTCCCACAATGGAACTGCATCTCCTATTAAGGGTAAGAAAAATGATCAGGTTCCGGCTGCACCAGGGTCAACATCTGGAAATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010020 GO:0016043 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043572 GO:0044422 GO:0044424 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

728

Amino Acids

80.12

Weight (kDa)

9.53

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL2_3	PF25237	149 - 278	2.9e-70	Mechanosensitive channel protein 2/3 domain
MS_channel_2nd	PF00924	280 - 350	2e-11	Mechanosensitive ion channel, beta-domain
Msl2-3_C	PF24956	355 - 441	1.9e-45	Mechanosensitive ion channel protein 2/3 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10490 AT5G10490 AT5G10490
fragaria_vesca	FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670 FvH4_1g19670
malus_domestica	MD15G1312300.v1.1
prunus_persica	Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1 Prupe.6G201500_v2.0.a1
pyrus_communis	pycom15g27600
rosa_chinensis	RchiOBHm_Chr2g0111221
rosa_laevigata	RLG00000017875
rosa_multiflora	Rmu_sc0003055.1_g000004
rosa_roxburghii	Rroxscaffold_2G00132610
rosa_rugosa	Rorug02G0171700 Rorug02G0171700 Rorug02G0171800
rosa_samantha	Rh2AG224800 Rh2BG236600 Rh2CG227800 Rh2DG232400
rosa_wichuraiana	Rw2G017360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	581, 977
AarI	CACCTGC	1 cut(s)	1661
AatII	GACGTC	1 cut(s)	1259
Acc16I	TGCGCA	1 cut(s)	1136
Acc36I	ACCTGC	1 cut(s)	1661
AccB1I	GGYRCC	1 cut(s)	1673
AccB7I	CCANNNNNTGG	2 cut(s)	37, 344
AccI	GTMKAC	1 cut(s)	183
AciI	CCGC	1 cut(s)	1546
AclWI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
AcoI	YGGCCR	1 cut(s)	1547
AcsI	RAATTY	3 cut(s)	647, 1696, 1804
AcyI	GRCGYC	3 cut(s)	1256, 1444, 1949
AfaI	GTAC	5 cut(s)	62, 676, 813, 1357, 1507
AfiI	CCNNNNNNNGG	6 cut(s)	37, 344, 783, 1330, 1545, 2126
AhdI	GACNNNNNGTC	1 cut(s)	1594
AjnI	CCWGG	2 cut(s)	549, 2158
AjuI	GAANNNNNNNTTGG	2 cut(s)	554, 586
AloI	GAACNNNNNNTCC	2 cut(s)	2101, 2133
AluBI	AGCT	4 cut(s)	272, 545, 1223, 1292
AluI	AGCT	4 cut(s)	272, 545, 1223, 1292
Alw26I	GTCTC	2 cut(s)	1798, 1806
AlwI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
AlwNI	CAGNNNCTG	1 cut(s)	1628
Ama87I	CYCGRG	1 cut(s)	1958
AoxI	GGCC	9 cut(s)	118, 188, 250, 346, 1332, 1536, 1547, 1566, 1976
ApeKI	GCWGC	5 cut(s)	272, 481, 737, 1168, 2153
ApoI	RAATTY	3 cut(s)	647, 1696, 1804
Asp700I	GAANNNNTTC	2 cut(s)	903, 999
AspLEI	GCGC	2 cut(s)	390, 1137
AspS9I	GGNCC	4 cut(s)	189, 406, 1333, 1714
AsuC2I	CCSGG	1 cut(s)	706
AsuHPI	GGTGA	5 cut(s)	835, 957, 995, 1070, 1952
AsuNHI	GCTAGC	1 cut(s)	545
AvaI	CYCGRG	1 cut(s)	1958
AvaII	GGWCC	2 cut(s)	406, 1714
BaeI	ACNNNNGTAYC	2 cut(s)	1608, 1641
BamHI	GGATCC	1 cut(s)	520
BanI	GGYRCC	1 cut(s)	1673
BanII	GRGCYC	1 cut(s)	2024
BarI	GAAGNNNNNNTAC	4 cut(s)	357, 389, 701, 733
BbsI	GAAGAC	3 cut(s)	272, 365, 1259
BbvI	GCAGC	5 cut(s)	259, 468, 724, 1180, 2140
BccI	CCATC	7 cut(s)	271, 346, 1094, 1332, 1523, 1558, 2047
BceAI	ACGGC	1 cut(s)	581
BciT130I	CCWGG	2 cut(s)	551, 2160
BclI	TGATCA	2 cut(s)	393, 2140
BcnI	CCSGG	1 cut(s)	706
BcoDI	GTCTC	2 cut(s)	1798, 1806
BfaI	CTAG	3 cut(s)	546, 764, 1809
BfmI	CTRYAG	4 cut(s)	537, 723, 792, 1983
BfuAI	ACCTGC	1 cut(s)	1661
BglI	GCCNNNNNGGC	2 cut(s)	258, 710
BglII	AGATCT	1 cut(s)	837
BisI	GCNGC	6 cut(s)	273, 482, 738, 1169, 1547, 2154
BlsI	GCNGC	6 cut(s)	274, 483, 739, 1170, 1548, 2155
BmcAI	AGTACT	2 cut(s)	676, 813
Bme1390I	CCNGG	3 cut(s)	551, 706, 2160
Bme18I	GGWCC	2 cut(s)	406, 1714
BmeRI	GACNNNNNGTC	1 cut(s)	1594
BmeT110I	CYCGRG	1 cut(s)	1958
BmgT120I	GGNCC	4 cut(s)	189, 406, 1333, 1714
BmiI	GGNNCC	6 cut(s)	336, 522, 1675, 1716, 2094, 2148
BmrFI	CCNGG	3 cut(s)	551, 706, 2160
BmsI	GCATC	2 cut(s)	1865, 2123
BmtI	GCTAGC	1 cut(s)	549
BpiI	GAAGAC	3 cut(s)	272, 365, 1259
BplI	GAGNNNNNCTC	2 cut(s)	486, 518
BpmI	CTGGAG	4 cut(s)	655, 1449, 1822, 1908
BpuEI	CTTGAG	1 cut(s)	1143
BpuMI	CCSGG	1 cut(s)	706
BsaAI	YACGTR	1 cut(s)	454
BsaHI	GRCGYC	3 cut(s)	1256, 1444, 1949
BsaI	GGTCTC	1 cut(s)	1798
BsaJI	CCNNGG	3 cut(s)	31, 1563, 2159
BsaXI	ACNNNNNCTCC	6 cut(s)	151, 181, 1906, 1936, 2101, 2131
Bsc4I	CCNNNNNNNGG	6 cut(s)	37, 344, 783, 1330, 1545, 2126
Bse118I	RCCGGY	1 cut(s)	116
Bse1I	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
Bse3DI	GCAATG	1 cut(s)	17
BseBI	CCWGG	2 cut(s)	551, 2160
BseDI	CCNNGG	3 cut(s)	31, 1563, 2159
BseGI	GGATG	1 cut(s)	1105
BseLI	CCNNNNNNNGG	6 cut(s)	37, 344, 783, 1330, 1545, 2126
BseMI	GCAATG	1 cut(s)	17
BseMII	CTCAG	1 cut(s)	1060
BseNI	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
BseRI	GAGGAG	1 cut(s)	2060
BseXI	GCAGC	5 cut(s)	259, 468, 724, 1180, 2140
BsgI	GTGCAG	2 cut(s)	1892, 2139
BshFI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BshNI	GGYRCC	1 cut(s)	1673
BsiHKCI	CYCGRG	1 cut(s)	1958
BsiSI	CCGG	4 cut(s)	117, 705, 1611, 2150
BsiWI	CGTACG	2 cut(s)	1355, 1505
BslFI	GGGAC	4 cut(s)	13, 54, 107, 820
BslI	CCNNNNNNNGG	6 cut(s)	37, 344, 783, 1330, 1545, 2126
BsmAI	GTCTC	2 cut(s)	1798, 1806
BsmFI	GGGAC	4 cut(s)	13, 54, 107, 820
BsnI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
Bso31I	GGTCTC	1 cut(s)	1798
BsoBI	CYCGRG	1 cut(s)	1958
Bsp1286I	GDGCHC	1 cut(s)	2024
Bsp19I	CCATGG	1 cut(s)	31
BspACI	CCGC	1 cut(s)	1546
BspANI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BspCNI	CTCAG	1 cut(s)	1059
BspLI	GGNNCC	6 cut(s)	336, 522, 1675, 1716, 2094, 2148
BspMAI	CTGCAG	2 cut(s)	727, 796
BspMI	ACCTGC	1 cut(s)	1661
BspOI	GCTAGC	1 cut(s)	549
BspPI	GGATC	6 cut(s)	515, 528, 1311, 1373, 1431, 1918
BspT107I	GGYRCC	1 cut(s)	1673
BspTNI	GGTCTC	1 cut(s)	1798
BsrDI	GCAATG	1 cut(s)	17
BsrFI	RCCGGY	1 cut(s)	116
BsrI	ACTGG	5 cut(s)	524, 672, 1018, 1515, 1925
BssAI	RCCGGY	1 cut(s)	116
BssECI	CCNNGG	3 cut(s)	31, 1563, 2159
BssNAI	GTATAC	1 cut(s)	184
BssNI	GRCGYC	3 cut(s)	1256, 1444, 1949
BssT1I	CCWWGG	2 cut(s)	31, 1563
Bst1107I	GTATAC	1 cut(s)	184
Bst2UI	CCWGG	2 cut(s)	551, 2160
Bst4CI	ACNGT	8 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771
Bst6I	CTCTTC	2 cut(s)	1180, 1263
BstACI	GRCGYC	3 cut(s)	1256, 1444, 1949
BstAPI	GCANNNNNTGC	1 cut(s)	1177
BstBAI	YACGTR	1 cut(s)	454
BstC8I	GCNNGC	2 cut(s)	547, 1980
BstDEI	CTNAG	2 cut(s)	1046, 1310
BstDSI	CCRYGG	1 cut(s)	31
BstEII	GGTNACC	1 cut(s)	963
BstF5I	GGATG	1 cut(s)	1105
BstHHI	GCGC	2 cut(s)	390, 1137
BstMAI	GTCTC	2 cut(s)	1798, 1806
BstMWI	GCNNNNNNNGC	6 cut(s)	258, 278, 551, 710, 1177, 1340
BstNI	CCWGG	2 cut(s)	551, 2160
BstNSI	RCATGY	2 cut(s)	953, 1135
BstPI	GGTNACC	1 cut(s)	963
BstSCI	CCNGG	3 cut(s)	549, 704, 2158
BstSFI	CTRYAG	4 cut(s)	537, 723, 792, 1983
BstV1I	GCAGC	5 cut(s)	259, 468, 724, 1180, 2140
BstV2I	GAAGAC	3 cut(s)	272, 365, 1259
BstX2I	RGATCY	4 cut(s)	520, 837, 1303, 1436
BstYI	RGATCY	4 cut(s)	520, 837, 1303, 1436
BstZ17I	GTATAC	1 cut(s)	184
BsuRI	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
BtgI	CCRYGG	1 cut(s)	31
BtgZI	GCGATG	2 cut(s)	290, 2066
BtsCI	GGATG	1 cut(s)	1105
BtsIMutI	CAGTG	6 cut(s)	1032, 1399, 1522, 1776, 1815, 1932
BveI	ACCTGC	1 cut(s)	1661
Cac8I	GCNNGC	2 cut(s)	547, 1980
CaiI	CAGNNNCTG	1 cut(s)	1628
CfoI	GCGC	2 cut(s)	390, 1137
Cfr10I	RCCGGY	1 cut(s)	116
Cfr13I	GGNCC	4 cut(s)	189, 406, 1333, 1714
CseI	GACGC	2 cut(s)	1452, 1957
Csp6I	GTAC	5 cut(s)	61, 675, 812, 1356, 1506
CviAII	CATG	8 cut(s)	32, 257, 754, 872, 950, 1132, 1424, 2072
CviQI	GTAC	5 cut(s)	61, 675, 812, 1356, 1506
DdeI	CTNAG	2 cut(s)	1046, 1310
DriI	GACNNNNNGTC	1 cut(s)	1594
EaeI	YGGCCR	1 cut(s)	1547
Eam1104I	CTCTTC	2 cut(s)	1180, 1263
Eam1105I	GACNNNNNGTC	1 cut(s)	1594
EarI	CTCTTC	2 cut(s)	1180, 1263
Eco130I	CCWWGG	2 cut(s)	31, 1563
Eco147I	AGGCCT	1 cut(s)	1978
Eco24I	GRGCYC	1 cut(s)	2024
Eco31I	GGTCTC	1 cut(s)	1798
Eco32I	GATATC	1 cut(s)	215
Eco47I	GGWCC	2 cut(s)	406, 1714
Eco88I	CYCGRG	1 cut(s)	1958
Eco91I	GGTNACC	1 cut(s)	963
EcoO109I	RGGNCCY	2 cut(s)	189, 1714
EcoO65I	GGTNACC	1 cut(s)	963
EcoRII	CCWGG	2 cut(s)	549, 2158
EcoRV	GATATC	1 cut(s)	215
EcoT14I	CCWWGG	2 cut(s)	31, 1563
EcoT38I	GRGCYC	1 cut(s)	2024
ErhI	CCWWGG	2 cut(s)	31, 1563
FaeI	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
FaqI	GGGAC	4 cut(s)	13, 54, 107, 820
FatI	CATG	8 cut(s)	31, 256, 753, 871, 949, 1131, 1423, 2071
FbaI	TGATCA	2 cut(s)	393, 2140
FblI	GTMKAC	1 cut(s)	183
Fnu4HI	GCNGC	6 cut(s)	273, 482, 738, 1169, 1547, 2154
FokI	GGATG	1 cut(s)	1112
FriOI	GRGCYC	1 cut(s)	2024
Fsp4HI	GCNGC	6 cut(s)	273, 482, 738, 1169, 1547, 2154
FspBI	CTAG	3 cut(s)	546, 764, 1809
FspI	TGCGCA	1 cut(s)	1136
GlaI	GCGC	2 cut(s)	389, 1136
GluI	GCNGC	6 cut(s)	273, 482, 738, 1169, 1547, 2154
GsuI	CTGGAG	4 cut(s)	655, 1449, 1822, 1908
HaeIII	GGCC	9 cut(s)	120, 190, 252, 348, 1334, 1538, 1549, 1568, 1978
HapII	CCGG	4 cut(s)	117, 705, 1611, 2150
HgaI	GACGC	2 cut(s)	1452, 1957
HhaI	GCGC	2 cut(s)	390, 1137
Hin1I	GRCGYC	3 cut(s)	1256, 1444, 1949
Hin1II	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
Hin6I	GCGC	2 cut(s)	388, 1135
HinP1I	GCGC	2 cut(s)	388, 1135
HincII	GTYRAC	4 cut(s)	285, 594, 1779, 2166
HindII	GTYRAC	4 cut(s)	285, 594, 1779, 2166
HpaII	CCGG	4 cut(s)	117, 705, 1611, 2150
HphI	GGTGA	5 cut(s)	835, 957, 995, 1070, 1952
Hpy166II	GTNNAC	8 cut(s)	184, 285, 594, 718, 1003, 1023, 1779, 2166
Hpy8I	GTNNAC	8 cut(s)	184, 285, 594, 718, 1003, 1023, 1779, 2166
HpyAV	CCTTC	9 cut(s)	419, 703, 914, 1528, 1714, 1931, 2045, 2073, 2106
HpyCH4III	ACNGT	8 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771
HpyCH4IV	ACGT	2 cut(s)	453, 1256
HpyF10VI	GCNNNNNNNGC	6 cut(s)	258, 278, 551, 710, 1177, 1340
HpyF3I	CTNAG	2 cut(s)	1046, 1310
HpySE526I	ACGT	2 cut(s)	453, 1256
Hsp92I	GRCGYC	3 cut(s)	1256, 1444, 1949
Hsp92II	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
HspAI	GCGC	2 cut(s)	388, 1135
Ksp22I	TGATCA	2 cut(s)	393, 2140
LmnI	GCTCC	4 cut(s)	494, 1430, 1613, 2098
Lsp1109I	GCAGC	5 cut(s)	259, 468, 724, 1180, 2140
LweI	GCATC	2 cut(s)	1865, 2123
MaeI	CTAG	3 cut(s)	546, 764, 1809
MaeII	ACGT	2 cut(s)	453, 1256
MaeIII	GTNAC	7 cut(s)	99, 454, 665, 963, 1204, 1257, 1757
MfeI	CAATTG	1 cut(s)	23
MflI	RGATCY	4 cut(s)	520, 837, 1303, 1436
MhlI	GDGCHC	1 cut(s)	2024
MlyI	GAGTC	8 cut(s)	662, 814, 871, 1323, 1510, 1553, 1625, 1829
MmeI	TCCRAC	2 cut(s)	410, 1281
MroXI	GAANNNNTTC	2 cut(s)	903, 999
MseI	TTAA	7 cut(s)	75, 110, 225, 558, 1284, 1785, 2124
MslI	CAYNNNNRTG	2 cut(s)	226, 1098
MspA1I	CMGCKG	1 cut(s)	272
MspI	CCGG	4 cut(s)	117, 705, 1611, 2150
MspR9I	CCNGG	3 cut(s)	551, 706, 2160
MunI	CAATTG	1 cut(s)	23
MvaI	CCWGG	2 cut(s)	551, 2160
MwoI	GCNNNNNNNGC	6 cut(s)	258, 278, 551, 710, 1177, 1340
NciI	CCSGG	1 cut(s)	706
NcoI	CCATGG	1 cut(s)	31
NheI	GCTAGC	1 cut(s)	545
NlaIII	CATG	8 cut(s)	35, 260, 757, 875, 953, 1135, 1427, 2075
NlaIV	GGNNCC	6 cut(s)	336, 522, 1675, 1716, 2094, 2148
NmuCI	GTSAC	5 cut(s)	454, 665, 963, 1257, 1757
NsbI	TGCGCA	1 cut(s)	1136
NspI	RCATGY	2 cut(s)	953, 1135
PaqCI	CACCTGC	1 cut(s)	1661
PceI	AGGCCT	1 cut(s)	1978
PdmI	GAANNNNTTC	2 cut(s)	903, 999
PfeI	GAWTC	8 cut(s)	52, 152, 631, 868, 904, 1409, 1601, 1829
Pfl23II	CGTACG	2 cut(s)	1355, 1505
PflMI	CCANNNNNTGG	2 cut(s)	37, 344
PkrI	GCNGC	6 cut(s)	274, 483, 739, 1170, 1548, 2155
PleI	GAGTC	8 cut(s)	662, 814, 871, 1322, 1509, 1553, 1625, 1829
PpsI	GAGTC	8 cut(s)	662, 814, 871, 1322, 1509, 1553, 1625, 1829
Ppu21I	YACGTR	1 cut(s)	454
PpuMI	RGGWCCY	1 cut(s)	1714
PsiI	TTATAA	2 cut(s)	581, 977
Psp5II	RGGWCCY	1 cut(s)	1714
Psp6I	CCWGG	2 cut(s)	549, 2158
PspEI	GGTNACC	1 cut(s)	963
PspGI	CCWGG	2 cut(s)	549, 2158
PspLI	CGTACG	2 cut(s)	1355, 1505
PspN4I	GGNNCC	6 cut(s)	336, 522, 1675, 1716, 2094, 2148
PspPI	GGNCC	4 cut(s)	189, 406, 1333, 1714
PspPPI	RGGWCCY	1 cut(s)	1714
PstI	CTGCAG	2 cut(s)	727, 796
PstNI	CAGNNNCTG	1 cut(s)	1628
PsuI	RGATCY	4 cut(s)	520, 837, 1303, 1436
PvuII	CAGCTG	1 cut(s)	272
RsaI	GTAC	5 cut(s)	62, 676, 813, 1357, 1507
RsaNI	GTAC	5 cut(s)	61, 675, 812, 1356, 1506
RseI	CAYNNNNRTG	2 cut(s)	226, 1098
SaqAI	TTAA	7 cut(s)	75, 110, 225, 558, 1284, 1785, 2124
SatI	GCNGC	6 cut(s)	273, 482, 738, 1169, 1547, 2154
Sau96I	GGNCC	4 cut(s)	189, 406, 1333, 1714
ScaI	AGTACT	2 cut(s)	676, 813
SchI	GAGTC	8 cut(s)	662, 814, 871, 1323, 1510, 1553, 1625, 1829
ScrFI	CCNGG	3 cut(s)	551, 706, 2160
SduI	GDGCHC	1 cut(s)	2024
SfaNI	GCATC	2 cut(s)	1865, 2123
SfcI	CTRYAG	4 cut(s)	537, 723, 792, 1983
SinI	GGWCC	2 cut(s)	406, 1714
SmiMI	CAYNNNNRTG	2 cut(s)	226, 1098
SmlI	CTYRAG	1 cut(s)	1158
SmoI	CTYRAG	1 cut(s)	1158
SseBI	AGGCCT	1 cut(s)	1978
SsiI	CCGC	1 cut(s)	1546
SspI	AATATT	1 cut(s)	1120
SspMI	CTAG	3 cut(s)	546, 764, 1809
StuI	AGGCCT	1 cut(s)	1978
StyD4I	CCNGG	3 cut(s)	549, 704, 2158
StyI	CCWWGG	2 cut(s)	31, 1563
TaaI	ACNGT	8 cut(s)	202, 598, 811, 943, 1027, 1360, 1394, 1771
TaiI	ACGT	2 cut(s)	456, 1259
TaqII	GACCGA	1 cut(s)	1780
TatI	WGTACW	2 cut(s)	674, 811
TauI	GCSGC	1 cut(s)	1549
TfiI	GAWTC	8 cut(s)	52, 152, 631, 868, 904, 1409, 1601, 1829
Tru1I	TTAA	7 cut(s)	75, 110, 225, 558, 1284, 1785, 2124
Tru9I	TTAA	7 cut(s)	75, 110, 225, 558, 1284, 1785, 2124
TscAI	CASTG	6 cut(s)	1032, 1399, 1522, 1776, 1822, 1932
TseFI	GTSAC	5 cut(s)	454, 665, 963, 1257, 1757
TseI	GCWGC	5 cut(s)	272, 481, 737, 1168, 2153
Tsp45I	GTSAC	5 cut(s)	454, 665, 963, 1257, 1757
TspDTI	ATGAA	6 cut(s)	6, 742, 860, 912, 1281, 1441
TspGWI	ACGGA	2 cut(s)	226, 352
TspRI	CASTG	6 cut(s)	1032, 1399, 1522, 1776, 1822, 1932
Van91I	CCANNNNNTGG	2 cut(s)	37, 344
VpaK11BI	GGWCC	2 cut(s)	406, 1714
XapI	RAATTY	3 cut(s)	647, 1696, 1804
XbaI	TCTAGA	1 cut(s)	1808
XceI	RCATGY	2 cut(s)	953, 1135
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1093
XmiI	GTMKAC	1 cut(s)	183
XmnI	GAANNNNTTC	2 cut(s)	903, 999
XspI	CTAG	3 cut(s)	546, 764, 1809
ZraI	GACGTC	1 cut(s)	1257
ZrmI	AGTACT	2 cut(s)	676, 813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.