Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Forward (+)

12348522 .. 12353463

Gene Structure 4942 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g20140.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2832 bp

ATGGGAAGCTTCAAATTCCTCAGCTTTTTAACTTCTAAAACCCACCCGAGGCTTCCGTACCAAAACCCCATTCGCCAAAATCTAGTAGACAAACCCGAAAGCCAGAATGCTCCCAACAGGGTGTTTAGATTCTTGCGCAGGCTTATGTCCGGAGGAATTATTGATGGGGGGTCGAGGACAGAGGCAGAGGAGAAGGTTTACTCTTGGTTATACGCCTTGGCGCAATCCGATAAGGATCTGGTTTATGAGTATGTTCGATCCACCGAAAGAGGGTTGAGCTTTACTGAAGCTGAAAGGAGATTGAAGGAAAATGGTCCGAATGTTCCTGTTGATTTCTCCTTCCCTCGCTGGTGGAATTTTTTATGGCATGCTTTCTTTCATCCTTTTAATATCATATTGATCATCCTGTCTGTAATCTCGTACATAACCAGTGACAGCCCAAATGGATGCATCATGCTTGTTTTGGTTTTGATAAGTGTTTGCCTCCGGTTTTATCAGGAATACGGAAGTTCAAAAGCAGCCATGGAACTTTCAGAATTTGTAAGGTGCCCAGTCAAAGTTCAAAGATGTGCAGGTAGAGTTGTTCAGACTGAATTAGTTGTTCAAATTGATCAAAGAGATATTGTTCCTGGTGATATTATCATATTTGAACCTGGAGACATTTTTCCTGGAGATGTGAGACTATTGTCCTCAAAACACCTTGTTGTAAGTCAGGCCTCGTTAACCGGAGAGTCCTGGACAACTGAGAAAACAGCTGATATCAGAGAAGATCAAAGCACACCATTGCTAGATTTAAGGAATATTTGCTTCATGGGAACAAATGTGGTATCAGGCAGTGGATCTGGTCTAGTGGTTTCCACTGGATCTAAGACATACATGAGCACCATGTTTTCAAACATAGGGAAAAAGAAACCACCAAATGACTTTGAGGACGGTATTCGTCGCATATCTTATGTGCTGGTTGCTGTTATGCTAGTGGTAGTCACCATCATAGTTATAACTGACTACTCTACATCTCAAGATCTGACTGAGAGCATCCTCTTCGGAGTGTCGGTTGCAAGTGCACTTACTCCTCAAATGCTTCCCCTGATTGTTAACACAAGTCTTGCAAAAGGAGCACTTGCTATGGCCAGAGATAGATGCATAGTCAAAAGCTTATCTGCAATACGAGACATGGGTTCTATGGATATCTTATGCATTGACAAGACTGGTACACTCACCATGAATCGTGCGATAATGGTAAATTATCTTGACAGCTGGGGGTTAAACAAAGAAAAAGTTTTACAGTTCGCTTTCCTCAACTCATATTTCAAGACCGATCAGAAATATCCTTTGGATGATGCAATTTTGGCACATGTATATACCAATGGATTCAGGTTCCAACCATCAAAATGGAAGAAACTAGATGAGATTCCTTTTGATTTCATAAGAAGAAGGGTATCTATTATCATGGAAAGAGAAGAAGAAAAACAAGAAGACAAAGACCCTCACGGTTTTGAGAGAGTCATGGTGACAAAAGGAGCTCTGGAAGAAGTAATGAAAGTTTGTTCTTTTATGGAGGATGTTGATAGTGGCACAATTTCACCTTTCTCTCCAGAAGAGTATCAAAGGATTATAAATATGACTGAGGAAATAAGCAATGAGGGACTAAGAGTTATAGGAGTAGCAACAAAGAAGCTCGAAAAGATAAGGTATGAGCGCAAAGATAATGATGATACTTCTGAATCAGACATGGTTTTCCTCGGCCTCATATCATTCTTTGACCCACCTAAGGACTCAGCAAAGCAAGCTCTGTGGCGGTTGGCTGAGAAGGGAGTGAAAGCAAAAGTATTAACAGGTGATTCACTGTCTCTATCTATAAGAGTTTGCAAGGAAGTAGGTATCAGGACAACTCATGTAGTTACAGGGCCAGAGCTTGAGCTACTCGATCACGATGCATTTCATGAGACTGTTAAAACAGCAACAGTCTTAGCTCGTCTCACCCCAACACAGAAACTCCGAGTTGTGCAGTCCTTGCAAACAGTTGGTAACCACATTGTTGGATTCTTGGGAGATGGAGTAAATGACTCGCTTGCATTGGATGCAGCGCATGTTGGTATATCCGTTGATTCAGGAGCATCAGTTGCAAAAGACTTTGCTGACATTATCTTACTGGAGAAGGACCTGAACGTACTCATTGCCGGAGTTGAACATGGCCGACTGACTTTCGGGAACACAATGAAGTACATAAAAATGTCGGTTATTGCCAATCTGGGAAGCGTTCTTTCAATCCTCATAGCAACTCTAGTGCTCAAGTACGAGCCATTGACTGCAAGGCAGCTTCTTACACAGAACTTCTTGTACAGTGTGGGCCAGATCGCAATACCATGGGACAAAATGGAAGAAGATTATGTAAAAGTCCCACAAAGATGGTCAAAGCAAGGTTTGCCAATGTTCATTCTATGGAATGGACCTGTCTGCACTCTTTTTGATGTTACTACACTTCTGTTCCTTTGGTTCTATTATAAGGCTGATAGTTTGGAGGATCTTATTTTCTTCCACACTGCTTGGTTCATAGAAGGGCTTCTCATGCAGACCCTAATCATCCACTTGATCCGTACGGAGAAAATTCCCTTCATTCAGGAATTTGCTTCATGGCCTGTGCTTTGTTCTACAGTTCTGATTTCTGCAATTGGAATTGCAATTCCATTCACTCCGATTGGGGAAGTGATGGGATTTATCAAGCTTCCACTGTCATACTTCGGTTTTTTGGTAGTGCTGTTTATAGGATATTTTGTTGTTGGCCAGTTGATCAAGAGACTTTACATTTTGGTTCATAAAAGCTGGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

944

Amino Acids

106.24

Weight (kDa)

6.18

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	78 - 140	1.9e-12	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	193 - 297	1.4e-17	P-type ATPase actuator domain
Cation_ATPase	PF13246	462 - 592	2.2e-22	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	743 - 909	7.6e-18	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	998, 1616, 2505
Acc16I	TGCGCA	1 cut(s)	137
Acc36I	ACCTGC	1 cut(s)	563
AccB1I	GGYRCC	1 cut(s)	546
AccI	GTMKAC	1 cut(s)	87
AccIII	TCCGGA	1 cut(s)	149
AciI	CCGC	1 cut(s)	1798
AclWI	GGATC	6 cut(s)	243, 252, 847, 871, 2532, 2587
AcoI	YGGCCR	3 cut(s)	1128, 2194, 2782
AcsI	RAATTY	5 cut(s)	14, 355, 536, 2607, 2624
AcuI	CTGAAG	1 cut(s)	306
AfaI	GTAC	8 cut(s)	59, 422, 1213, 2172, 2225, 2297, 2342, 2599
AfiI	CCNNNNNNNGG	3 cut(s)	48, 270, 1771
AflIII	ACRYGT	1 cut(s)	1354
AjnI	CCWGG	4 cut(s)	628, 652, 667, 734
AjuI	GAANNNNNNNTTGG	4 cut(s)	54, 86, 1316, 1348
Alw21I	GWGCWC	5 cut(s)	884, 1066, 1120, 1525, 2292
Alw26I	GTCTC	7 cut(s)	651, 673, 1164, 1854, 1940, 1982, 2791
Alw44I	GTGCAC	1 cut(s)	1062
AlwI	GGATC	6 cut(s)	243, 252, 847, 871, 2532, 2587
Ama87I	CYCGRG	1 cut(s)	46
Aor13HI	TCCGGA	1 cut(s)	149
AoxI	GGCC	8 cut(s)	714, 1128, 1744, 1907, 2194, 2350, 2636, 2782
ApaLI	GTGCAC	1 cut(s)	1062
ApeKI	GCWGC	3 cut(s)	518, 2084, 2317
ApoI	RAATTY	5 cut(s)	14, 355, 536, 2607, 2624
Asp700I	GAANNNNTTC	1 cut(s)	2562
AspLEI	GCGC	4 cut(s)	138, 223, 1701, 2089
AspS9I	GGNCC	5 cut(s)	314, 1907, 2161, 2350, 2450
AsuHPI	GGTGA	7 cut(s)	644, 976, 1210, 1522, 1575, 1850, 1972
AvaI	CYCGRG	1 cut(s)	46
AvaII	GGWCC	3 cut(s)	314, 2161, 2450
AxyI	CCTNAGG	1 cut(s)	1770
BaeGI	GKGCMC	2 cut(s)	551, 1066
BalI	TGGCCA	2 cut(s)	1130, 2784
BanI	GGYRCC	1 cut(s)	546
BanII	GRGCYC	1 cut(s)	1525
BbsI	GAAGAC	1 cut(s)	1482
Bbv12I	GWGCWC	5 cut(s)	884, 1066, 1120, 1525, 2292
BbvCI	CCTCAGC	1 cut(s)	20
BbvI	GCAGC	3 cut(s)	530, 2096, 2329
BccI	CCATC	6 cut(s)	158, 995, 1393, 2048, 2403, 2704
BcgI	CGANNNNNNTGC	4 cut(s)	1024, 1058, 1916, 1950
BciT130I	CCWGG	4 cut(s)	630, 654, 669, 736
BclI	TGATCA	3 cut(s)	399, 610, 2790
BcoDI	GTCTC	7 cut(s)	651, 673, 1164, 1854, 1940, 1982, 2791
BfaI	CTAG	6 cut(s)	83, 788, 848, 974, 1403, 2285
BfmI	CTRYAG	2 cut(s)	2652, 2828
BfuAI	ACCTGC	1 cut(s)	563
BglII	AGATCT	1 cut(s)	1021
BisI	GCNGC	3 cut(s)	519, 2085, 2318
BlsI	GCNGC	3 cut(s)	520, 2086, 2319
Bme1390I	CCNGG	4 cut(s)	630, 654, 669, 736
Bme18I	GGWCC	3 cut(s)	314, 2161, 2450
BmeT110I	CYCGRG	1 cut(s)	46
BmgT120I	GGNCC	5 cut(s)	314, 1907, 2161, 2350, 2450
BmiI	GGNNCC	2 cut(s)	548, 1379
BmrFI	CCNGG	4 cut(s)	630, 654, 669, 736
BmrI	ACTGGG	1 cut(s)	545
BmsI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1924, 2071, 2126
BmuI	ACTGGG	1 cut(s)	545
BoxI	GACNNNNGTC	1 cut(s)	685
BpiI	GAAGAC	1 cut(s)	1482
BpmI	CTGGAG	4 cut(s)	675, 690, 1578, 2174
Bpu10I	CCTNAGC	1 cut(s)	20
BpuEI	CTTGAG	3 cut(s)	1002, 1937, 2276
BsaBI	GATNNNNATC	1 cut(s)	234
BsaJI	CCNNGG	5 cut(s)	47, 216, 522, 1741, 2366
BsaWI	WCCGGW	3 cut(s)	149, 486, 725
BsaXI	ACNNNNNCTCC	8 cut(s)	1038, 1068, 1980, 2010, 2043, 2073, 2106, 2136
Bsc4I	CCNNNNNNNGG	3 cut(s)	48, 270, 1771
Bse1I	ACTGG	6 cut(s)	429, 551, 865, 1213, 2157, 2785
Bse21I	CCTNAGG	1 cut(s)	1770
Bse3DI	GCAATG	3 cut(s)	782, 1645, 2175
Bse8I	GATNNNNATC	1 cut(s)	234
BseAI	TCCGGA	1 cut(s)	149
BseBI	CCWGG	4 cut(s)	630, 654, 669, 736
BseDI	CCNNGG	5 cut(s)	47, 216, 522, 1741, 2366
BseGI	GGATG	8 cut(s)	379, 402, 452, 1035, 1342, 1567, 2086, 2583
BseJI	GATNNNNATC	1 cut(s)	234
BseLI	CCNNNNNNNGG	3 cut(s)	48, 270, 1771
BseMI	GCAATG	3 cut(s)	782, 1645, 2175
BseMII	CTCAG	6 cut(s)	34, 735, 1020, 1617, 1791, 1797
BseNI	ACTGG	6 cut(s)	429, 551, 865, 1213, 2157, 2785
BseRI	GAGGAG	2 cut(s)	203, 1062
BseSI	GKGCMC	2 cut(s)	551, 1066
BseXI	GCAGC	3 cut(s)	530, 2096, 2329
BseYI	CCCAGC	1 cut(s)	1257
BsgI	GTGCAG	3 cut(s)	591, 2027, 2443
BshFI	GGCC	8 cut(s)	716, 1130, 1746, 1909, 2196, 2352, 2638, 2784
BshNI	GGYRCC	1 cut(s)	546
BsiHKAI	GWGCWC	5 cut(s)	884, 1066, 1120, 1525, 2292
BsiHKCI	CYCGRG	1 cut(s)	46
BsiSI	CCGG	4 cut(s)	150, 487, 726, 2181
BsiWI	CGTACG	1 cut(s)	2597
BslFI	GGGAC	3 cut(s)	1659, 2384, 2384
BslI	CCNNNNNNNGG	3 cut(s)	48, 270, 1771
BsmAI	GTCTC	7 cut(s)	651, 673, 1164, 1854, 1940, 1982, 2791
BsmBI	CGTCTC	1 cut(s)	1982
BsmFI	GGGAC	3 cut(s)	1659, 2384, 2384
BsmI	GAATGC	1 cut(s)	112
BsnI	GGCC	8 cut(s)	716, 1130, 1746, 1909, 2196, 2352, 2638, 2784
BsoBI	CYCGRG	1 cut(s)	46
Bsp1286I	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1525, 2292
Bsp13I	TCCGGA	1 cut(s)	149
Bsp1407I	TGTACA	1 cut(s)	2340
Bsp19I	CCATGG	2 cut(s)	522, 2366
BspACI	CCGC	1 cut(s)	1798
BspANI	GGCC	8 cut(s)	716, 1130, 1746, 1909, 2196, 2352, 2638, 2784
BspCNI	CTCAG	6 cut(s)	33, 736, 1021, 1618, 1790, 1798
BspEI	TCCGGA	1 cut(s)	149
BspHI	TCATGA	1 cut(s)	1942
BspLI	GGNNCC	2 cut(s)	548, 1379
BspMI	ACCTGC	1 cut(s)	563
BspPI	GGATC	6 cut(s)	243, 252, 847, 871, 2532, 2587
BspT107I	GGYRCC	1 cut(s)	546
BsrDI	GCAATG	3 cut(s)	782, 1645, 2175
BsrGI	TGTACA	1 cut(s)	2340
BsrI	ACTGG	6 cut(s)	429, 551, 865, 1213, 2157, 2785
BssECI	CCNNGG	5 cut(s)	47, 216, 522, 1741, 2366
BssT1I	CCWWGG	3 cut(s)	216, 522, 2366
Bst2UI	CCWGG	4 cut(s)	630, 654, 669, 736
Bst6I	CTCTTC	2 cut(s)	1046, 1593
BstAPI	GCANNNNNTGC	4 cut(s)	2014, 2081, 2123, 2425
BstAUI	TGTACA	1 cut(s)	2340
BstC8I	GCNNGC	5 cut(s)	140, 369, 1788, 2073, 2825
BstDSI	CCRYGG	2 cut(s)	522, 2366
BstEII	GGTNACC	1 cut(s)	2027
BstF5I	GGATG	8 cut(s)	379, 402, 452, 1035, 1342, 1567, 2086, 2583
BstHHI	GCGC	4 cut(s)	138, 223, 1701, 2089
BstMAI	GTCTC	7 cut(s)	651, 673, 1164, 1854, 1940, 1982, 2791
BstMWI	GCNNNNNNNGC	8 cut(s)	1115, 1349, 1787, 2014, 2081, 2123, 2425, 2569
BstNI	CCWGG	4 cut(s)	630, 654, 669, 736
BstNSI	RCATGY	3 cut(s)	371, 1358, 2093
BstPAI	GACNNNNGTC	1 cut(s)	685
BstPI	GGTNACC	1 cut(s)	2027
BstSCI	CCNGG	4 cut(s)	628, 652, 667, 734
BstSFI	CTRYAG	2 cut(s)	2652, 2828
BstSLI	GKGCMC	2 cut(s)	551, 1066
BstV1I	GCAGC	3 cut(s)	530, 2096, 2329
BstV2I	GAAGAC	1 cut(s)	1482
BstX2I	RGATCY	5 cut(s)	235, 839, 863, 1021, 2524
BstXI	CCANNNNNNTGG	3 cut(s)	1392, 2039, 2409
BstYI	RGATCY	5 cut(s)	235, 839, 863, 1021, 2524
Bsu36I	CCTNAGG	1 cut(s)	1770
BsuRI	GGCC	8 cut(s)	716, 1130, 1746, 1909, 2196, 2352, 2638, 2784
BtgI	CCRYGG	2 cut(s)	522, 2366
BtsCI	GGATG	8 cut(s)	379, 402, 452, 1035, 1342, 1567, 2086, 2583
BtsI	GCAGTG	2 cut(s)	841, 2541
BtsIMutI	CAGTG	7 cut(s)	436, 841, 858, 1844, 2350, 2541, 2729
BveI	ACCTGC	1 cut(s)	563
Cac8I	GCNNGC	5 cut(s)	140, 369, 1788, 2073, 2825
CciI	TCATGA	1 cut(s)	1942
CfoI	GCGC	4 cut(s)	138, 223, 1701, 2089
Cfr13I	GGNCC	5 cut(s)	314, 1907, 2161, 2350, 2450
Csp6I	GTAC	8 cut(s)	58, 421, 1212, 2171, 2224, 2296, 2341, 2598
CspCI	CAANNNNNGTGG	4 cut(s)	1775, 1810, 2528, 2563
CviQI	GTAC	8 cut(s)	58, 421, 1212, 2171, 2224, 2296, 2341, 2598
EaeI	YGGCCR	3 cut(s)	1128, 2194, 2782
Eam1104I	CTCTTC	2 cut(s)	1046, 1593
EarI	CTCTTC	2 cut(s)	1046, 1593
Ecl136II	GAGCTC	1 cut(s)	1523
Eco130I	CCWWGG	3 cut(s)	216, 522, 2366
Eco147I	AGGCCT	1 cut(s)	716
Eco24I	GRGCYC	1 cut(s)	1525
Eco32I	GATATC	2 cut(s)	760, 1189
Eco47I	GGWCC	3 cut(s)	314, 2161, 2450
Eco53kI	GAGCTC	1 cut(s)	1523
Eco57I	CTGAAG	1 cut(s)	306
Eco81I	CCTNAGG	1 cut(s)	1770
Eco88I	CYCGRG	1 cut(s)	46
Eco91I	GGTNACC	1 cut(s)	2027
EcoICRI	GAGCTC	1 cut(s)	1523
EcoO109I	RGGNCCY	1 cut(s)	2161
EcoO65I	GGTNACC	1 cut(s)	2027
EcoRII	CCWGG	4 cut(s)	628, 652, 667, 734
EcoRV	GATATC	2 cut(s)	760, 1189
EcoT14I	CCWWGG	3 cut(s)	216, 522, 2366
EcoT22I	ATGCAT	4 cut(s)	452, 1145, 1199, 1939
EcoT38I	GRGCYC	1 cut(s)	1525
ErhI	CCWWGG	3 cut(s)	216, 522, 2366
Esp3I	CGTCTC	1 cut(s)	1982
FalI	AAGNNNNNCTT	2 cut(s)	1104, 1136
FaqI	GGGAC	3 cut(s)	1659, 2384, 2384
FbaI	TGATCA	3 cut(s)	399, 610, 2790
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	3 cut(s)	519, 2085, 2318
FokI	GGATG	8 cut(s)	366, 389, 459, 1022, 1349, 1574, 2093, 2570
FriOI	GRGCYC	1 cut(s)	1525
Fsp4HI	GCNGC	3 cut(s)	519, 2085, 2318
FspBI	CTAG	6 cut(s)	83, 788, 848, 974, 1403, 2285
FspI	TGCGCA	1 cut(s)	137
GlaI	GCGC	4 cut(s)	137, 222, 1700, 2088
GluI	GCNGC	3 cut(s)	519, 2085, 2318
GsaI	CCCAGC	1 cut(s)	1261
GsuI	CTGGAG	4 cut(s)	675, 690, 1578, 2174
HaeIII	GGCC	8 cut(s)	716, 1130, 1746, 1909, 2196, 2352, 2638, 2784
HapII	CCGG	4 cut(s)	150, 487, 726, 2181
HhaI	GCGC	4 cut(s)	138, 223, 1701, 2089
Hin6I	GCGC	4 cut(s)	136, 221, 1699, 2087
HinP1I	GCGC	4 cut(s)	136, 221, 1699, 2087
HincII	GTYRAC	2 cut(s)	723, 1096
HindII	GTYRAC	2 cut(s)	723, 1096
HindIII	AAGCTT	3 cut(s)	7, 1153, 2723
HpaI	GTTAAC	2 cut(s)	723, 1096
HpaII	CCGG	4 cut(s)	150, 487, 726, 2181
HphI	GGTGA	7 cut(s)	644, 976, 1210, 1522, 1575, 1850, 1972
Hpy166II	GTNNAC	6 cut(s)	88, 199, 723, 1064, 1096, 1214
Hpy8I	GTNNAC	6 cut(s)	88, 199, 723, 1064, 1096, 1214
Hpy99I	CGWCG	1 cut(s)	945
HpyAV	CCTTC	8 cut(s)	187, 298, 349, 1428, 1804, 2152, 2552, 2623
HpyCH4IV	ACGT	1 cut(s)	2169
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1115, 1349, 1787, 2014, 2081, 2123, 2425, 2569
HpySE526I	ACGT	1 cut(s)	2169
HspAI	GCGC	4 cut(s)	136, 221, 1699, 2087
Kpn2I	TCCGGA	1 cut(s)	149
Ksp22I	TGATCA	3 cut(s)	399, 610, 2790
KspAI	GTTAAC	2 cut(s)	723, 1096
LmnI	GCTCC	4 cut(s)	115, 1115, 1520, 2114
Lsp1109I	GCAGC	3 cut(s)	530, 2096, 2329
LweI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1924, 2071, 2126
MaeI	CTAG	6 cut(s)	83, 788, 848, 974, 1403, 2285
MaeII	ACGT	1 cut(s)	2169
MaeIII	GTNAC	6 cut(s)	431, 982, 1510, 1900, 2027, 2473
MfeI	CAATTG	1 cut(s)	2670
MflI	RGATCY	5 cut(s)	235, 839, 863, 1021, 2524
MhlI	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1525, 2292
MlsI	TGGCCA	2 cut(s)	1130, 2784
MluNI	TGGCCA	2 cut(s)	1130, 2784
MlyI	GAGTC	4 cut(s)	740, 1512, 1769, 2060
MmeI	TCCRAC	2 cut(s)	1405, 2020
Mox20I	TGGCCA	2 cut(s)	1130, 2784
Mph1103I	ATGCAT	4 cut(s)	452, 1145, 1199, 1939
MroI	TCCGGA	1 cut(s)	149
MroXI	GAANNNNTTC	1 cut(s)	2562
MscI	TGGCCA	2 cut(s)	1130, 2784
MseI	TTAA	8 cut(s)	29, 387, 722, 794, 1095, 1265, 1832, 1953
MslI	CAYNNNNRTG	3 cut(s)	1390, 2231, 2407
Msp20I	TGGCCA	2 cut(s)	1130, 2784
MspA1I	CMGCKG	2 cut(s)	755, 1257
MspI	CCGG	4 cut(s)	150, 487, 726, 2181
MspR9I	CCNGG	4 cut(s)	630, 654, 669, 736
MunI	CAATTG	1 cut(s)	2670
Mva1269I	GAATGC	1 cut(s)	112
MvaI	CCWGG	4 cut(s)	630, 654, 669, 736
MwoI	GCNNNNNNNGC	8 cut(s)	1115, 1349, 1787, 2014, 2081, 2123, 2425, 2569
NcoI	CCATGG	2 cut(s)	522, 2366
NlaIV	GGNNCC	2 cut(s)	548, 1379
NmeAIII	GCCGAG	1 cut(s)	1722
NmuCI	GTSAC	3 cut(s)	431, 982, 1510
NsbI	TGCGCA	1 cut(s)	137
NsiI	ATGCAT	4 cut(s)	452, 1145, 1199, 1939
NspI	RCATGY	3 cut(s)	371, 1358, 2093
PaeI	GCATGC	1 cut(s)	371
PagI	TCATGA	1 cut(s)	1942
PceI	AGGCCT	1 cut(s)	716
PciI	ACATGT	1 cut(s)	1354
PctI	GAATGC	1 cut(s)	112
PdmI	GAANNNNTTC	1 cut(s)	2562
PfeI	GAWTC	8 cut(s)	129, 1225, 1371, 1411, 1724, 1841, 2043, 2108
Pfl23II	CGTACG	1 cut(s)	2597
PfoI	TCCNGGA	2 cut(s)	667, 734
PkrI	GCNGC	3 cut(s)	520, 2086, 2319
PleI	GAGTC	4 cut(s)	739, 1511, 1769, 2060
PpsI	GAGTC	4 cut(s)	739, 1511, 1769, 2060
PpuMI	RGGWCCY	1 cut(s)	2161
PscI	ACATGT	1 cut(s)	1354
PshAI	GACNNNNGTC	1 cut(s)	685
PsiI	TTATAA	3 cut(s)	998, 1616, 2505
Psp124BI	GAGCTC	1 cut(s)	1525
Psp5II	RGGWCCY	1 cut(s)	2161
Psp6I	CCWGG	4 cut(s)	628, 652, 667, 734
PspEI	GGTNACC	1 cut(s)	2027
PspFI	CCCAGC	1 cut(s)	1257
PspGI	CCWGG	4 cut(s)	628, 652, 667, 734
PspLI	CGTACG	1 cut(s)	2597
PspN4I	GGNNCC	2 cut(s)	548, 1379
PspPI	GGNCC	5 cut(s)	314, 1907, 2161, 2350, 2450
PspPPI	RGGWCCY	1 cut(s)	2161
PsrI	GAACNNNNNNTAC	2 cut(s)	2324, 2356
PsuI	RGATCY	5 cut(s)	235, 839, 863, 1021, 2524
PvuII	CAGCTG	2 cut(s)	755, 1257
RsaI	GTAC	8 cut(s)	59, 422, 1213, 2172, 2225, 2297, 2342, 2599
RsaNI	GTAC	8 cut(s)	58, 421, 1212, 2171, 2224, 2296, 2341, 2598
RseI	CAYNNNNRTG	3 cut(s)	1390, 2231, 2407
SacI	GAGCTC	1 cut(s)	1525
SaqAI	TTAA	8 cut(s)	29, 387, 722, 794, 1095, 1265, 1832, 1953
SatI	GCNGC	3 cut(s)	519, 2085, 2318
Sau96I	GGNCC	5 cut(s)	314, 1907, 2161, 2350, 2450
SchI	GAGTC	4 cut(s)	740, 1512, 1769, 2060
ScrFI	CCNGG	4 cut(s)	630, 654, 669, 736
SduI	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1525, 2292
SfaNI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1924, 2071, 2126
SfcI	CTRYAG	2 cut(s)	2652, 2828
SinI	GGWCC	3 cut(s)	314, 2161, 2450
SmiMI	CAYNNNNRTG	3 cut(s)	1390, 2231, 2407
SmlI	CTYRAG	3 cut(s)	1017, 1916, 2291
SmoI	CTYRAG	3 cut(s)	1017, 1916, 2291
SphI	GCATGC	1 cut(s)	371
SseBI	AGGCCT	1 cut(s)	716
SsiI	CCGC	1 cut(s)	1798
SspI	AATATT	1 cut(s)	802
SspMI	CTAG	6 cut(s)	83, 788, 848, 974, 1403, 2285
SstI	GAGCTC	1 cut(s)	1525
StuI	AGGCCT	1 cut(s)	716
StyD4I	CCNGG	4 cut(s)	628, 652, 667, 734
StyI	CCWWGG	3 cut(s)	216, 522, 2366
TaiI	ACGT	1 cut(s)	2172
TaqI	TCGA	4 cut(s)	173, 256, 1680, 1926
TaqII	GACCGA	1 cut(s)	1331
TatI	WGTACW	2 cut(s)	2223, 2340
TfiI	GAWTC	8 cut(s)	129, 1225, 1371, 1411, 1724, 1841, 2043, 2108
Tru1I	TTAA	8 cut(s)	29, 387, 722, 794, 1095, 1265, 1832, 1953
Tru9I	TTAA	8 cut(s)	29, 387, 722, 794, 1095, 1265, 1832, 1953
TscAI	CASTG	7 cut(s)	436, 841, 865, 1851, 2350, 2548, 2736
TseFI	GTSAC	3 cut(s)	431, 982, 1510
TseI	GCWGC	3 cut(s)	518, 2084, 2317
Tsp45I	GTSAC	3 cut(s)	431, 982, 1510
TspGWI	ACGGA	5 cut(s)	45, 519, 2092, 2585, 2615
TspRI	CASTG	7 cut(s)	436, 841, 865, 1851, 2350, 2548, 2736
VneI	GTGCAC	1 cut(s)	1062
VpaK11BI	GGWCC	3 cut(s)	314, 2161, 2450
XapI	RAATTY	5 cut(s)	14, 355, 536, 2607, 2624
XceI	RCATGY	3 cut(s)	371, 1358, 2093
XmiI	GTMKAC	1 cut(s)	87
XmnI	GAANNNNTTC	1 cut(s)	2562
XspI	CTAG	6 cut(s)	83, 788, 848, 974, 1403, 2285
Zsp2I	ATGCAT	4 cut(s)	452, 1145, 1199, 1939

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.