Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Forward (+)

24862184 .. 24867038

Gene Structure 4855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG231200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

ATGCTAACTTTCTCTTCATCTTACTTGTCATTCGATACATATTATGTCTCTCTTTCATTCTCTACGGCCATTGCTATTTCTGGTTCTCTGCGAATGAGAATTTTCAAAATCCCCACCATTTTTACTTCTAAAAGCCACCCGAGGCTTCCTTACCAAAACCCCATTCGCCAAAATCTAGTAGACAAACCTGAAAGCCAGAATGGCTCCAACAGGGTGTTTAGATTCTTGCGCGGGCTTATGTCCGGAGGGAAAATTGATGGGGGGTCGAGGACAGAGGCAGAGGAGAAGCTTTACTCTTGGTTATACGCCTTGGCGCAATCCGATAAGGATTTGGTTTTCGAGTATGTTCGATCCACCGAAAGAGGATTGAGCTTTACTGAAGCTGAAAGGAGATTGAAGGAAAATGGCCCGAATGTTCCTGTTGATTTCTCTTTTCCTAGCTGGTGGCATTTTTTATGGAGTGCTTTCTTTCATCCTTTTAATATCATATTGATCGTCCTGTCTGTAATCTCGTACATAACCAGCGACAGCCCAAATGGATGCATCATGCTTGTTTTGGTTTTGATAAGTGTTTGCCTCCGGTTCTATCAGGAATACGGAAGTTCAAAAGCAGCCATGGAACTTTCAGAATTTGTAAGGTGCCCAGTCAAAGTTCAAAGATGTGCAGGTAGAGTTGTTCAGACTGAATTAGTAGTACAAATTGATCAAAGAGATATTGTTCCTGGTGATATTATCATATTTGAACCTGGAGACCTTTTTCCTGGAGATGTGAGACTATTGTCTTCGAAACACCTTGTTGTAAGTCAGGCCTCATTAACAGGAGAGTCCTGGACAACCGAAAAAACAGCTGATATCAGAGAGAATCAAAGCACTCCATTGCTAGATTTAAGGAATATTTGCTTCATGGGAACAAATGTAGTATCAGGCAGTGGATCTGGTCTAGTGGTTTCCACTGGATCTAAGACATACATGAGCACCATGTTTTCAAACATAGGGAAGAAGAAACCACCAAATGAATTTGAGGACGGTGTTCGTCGCATATCTTATGTGCTGGTTGCTGTTATGCTAGTAGTAGTCACCATCATAGTTATAACTGACTACTCTACATCTCTTGATCTGTCTGAGAGCATCCTTTTTGGAGTGTCAGTTGCAAGTGCACTTACTCCTCAAATGCTTCCCCTGGTCGTTAACACAAGTCTTGCAAAAGGAGCACTTGCTATGGCCAGAGACAGATGCATAATCAAAAGCTTATCTGCAATACGAAACATGGGTTCTATGGATATCTTATGCATTGACAAGACTGGTACACTCACCATGAATCGTGCGATAATGGTTAATTATCTGGACAGCTGGGGGTTAGACAAAGAAAAGGTTTTACAGTTTGCTTTCCTCAACTCATATTTCAAGACCGATCAGAAATATCCTTTGGATGATGCAATTTTGGCACATGTATATACCAATGGATTCAGGTTCCAACCGTCAAAATGGAAGAAACTAGATGAGATTCCTTTTGATTTCATAAGAAGAAGGGTATCTATTATCATGGAAAGAGAAGAAGACACAGACCCTCACAGTTTTGTGAGAGTCATGGTGACAAAAGGAGCTCTGGAAGAAGTAATGAAAGTTTGTTCTTGTATGGAAAATGTTGACAGTGGCACAATTTCACCTCTCTCTCCAGAACAGTATCAAAGGATTATAAATATGACCGAAGAAATAAGCAATGAGGGACTAAGAGTTATAGGAGTAGCAACAAAGAAGCTGGGAAAGCAGATAAGGTATGAGCGCAAAGATAATGATGATACTTCTGAATCAGACATGGTTTTCCTCGGCCTCATTACATTCTTTGACCCACCCAAGGACTCAGCAAAGCAAGCTCTGTGGCGGTTGGCTGAGAAGGGAGTGAAAGCAAAAGTATTAACAGGTGACTCACTGTCTCTATCTATAAGAGTTTGCAAGGAAGTTGGTATCAGAACAACTCATGTAGTTACGGGGCCAGAGCTTGAGCTACTTGACCAGGATGCCTTTCATGAGACTGTTAAAACAGCAACGGTCTTAGCTCGACTCACCCCAACGCAGAAACTCCGAGTTGTGCAATCCTTGCAAACAATTGGTAACCACATTGTTGGATTTTTGGGAGATGGAGTAAATGACTCGCTTGCACTCGATGCAGCCCATGTTGGTATATCAGTTGATTCAGGAGCATCAGTTGCAAAAGACTTTGCTGACATTATCTTACTGGAGAAAGACCTGAATGTACTCATTGCCGGAGTTGAACATGGCCGACTCACTTTTGGGAACACAATGAAGTACATAAAAATGTCAGTTATTGCCAATCTGGGAAGCGTTCTCTCAATTCTCATAGCAACCCTGGTGCTCAAGTATGAGCCATTGACGGCAAGGCAGCTTCTTACACAGAACTTCTTGTATAGTGTGGGCCAGATTGCAATCCCATGGGATAAAATGGAAGATGATTATGTAAAAGTCCCACAAAGATGGTCAAAGAAAGGTTTGCCGACGTTCATTTTGTGGAATGGACCTGTCTGCACTCTTTTTGATGTTACTACACTTCTGTTCCTTTGGTTCTATTATAAGGCTGATAATCTGGAGGATCTTGTTTTCTTCCACACTGCTTGGTTCATCGAAGGGCTTCTCATGCAGACCCTAATCATCCACTTGATCCGTACAGAGAAAATTCCTTTCATTCAGGAGTTTGCCTCATGGCCTGTGCTTTGTTCTACAGTTCTGGTTTCTGCAATTGGAATCGCAATTACATTCACCCCGATTGGGAAAGTGATGGGATTTATCAGGCTTCCACTGTCATACTTTGGGTTTTTGGTAGTACTGTTTATAGGATATTTTGTTGTTGGCCAGGTGGTCAAGAGACTCTACATTTTGGTTTATAAAACCTGGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

971

Amino Acids

108.92

Weight (kDa)

7.52

Isoelectric Point (pI)

34.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	109 - 171	8.8e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	224 - 328	3.4e-17	P-type ATPase actuator domain
Cation_ATPase	PF13246	493 - 620	5.3e-22	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	508 - 724	1.1e-07	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	796 - 962	7.7e-19	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1091, 1697, 2589, 2901
Acc36I	ACCTGC	1 cut(s)	656
AccB1I	GGYRCC	1 cut(s)	639
AccI	GTMKAC	1 cut(s)	180
AccII	CGCG	1 cut(s)	231
AccIII	TCCGGA	1 cut(s)	242
AciI	CCGC	2 cut(s)	231, 1882
AclWI	GGATC	5 cut(s)	345, 940, 964, 2616, 2671
AcoI	YGGCCR	4 cut(s)	66, 1221, 2278, 2866
AcsI	RAATTY	4 cut(s)	99, 629, 1016, 2691
AcuI	CTGAAG	1 cut(s)	399
AfaI	GTAC	7 cut(s)	515, 696, 1306, 2256, 2309, 2683, 2841
AfiI	CCNNNNNNNGG	2 cut(s)	1855, 2784
AflIII	ACRYGT	1 cut(s)	1447
AgsI	TTSAA	8 cut(s)	106, 397, 606, 656, 743, 987, 1405, 2273
AhdI	GACNNNNNGTC	1 cut(s)	1930
AjnI	CCWGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
AjuI	GAANNNNNNNTTGG	4 cut(s)	147, 179, 1409, 1441
Alw21I	GWGCWC	5 cut(s)	977, 1159, 1213, 1606, 2376
Alw26I	GTCTC	7 cut(s)	52, 744, 766, 1221, 1938, 2024, 2875
Alw44I	GTGCAC	1 cut(s)	1155
AlwI	GGATC	5 cut(s)	345, 940, 964, 2616, 2671
Ama87I	CYCGRG	1 cut(s)	139
Aor13HI	TCCGGA	1 cut(s)	242
ApaLI	GTGCAC	1 cut(s)	1155
ApeKI	GCWGC	3 cut(s)	611, 2168, 2401
ApoI	RAATTY	4 cut(s)	99, 629, 1016, 2691
ArsI	GACNNNNNNTTYG	2 cut(s)	2506, 2538
Asp700I	GAANNNNTTC	1 cut(s)	2646
AspLEI	GCGC	3 cut(s)	231, 316, 1785
AspS9I	GGNCC	4 cut(s)	407, 1991, 2434, 2534
AsuHPI	GGTGA	8 cut(s)	737, 1069, 1303, 1603, 1656, 1934, 2056, 2767
AsuII	TTCGAA	1 cut(s)	785
AvaI	CYCGRG	1 cut(s)	139
AvaII	GGWCC	1 cut(s)	2534
BaeGI	GKGCMC	2 cut(s)	644, 1159
BalI	TGGCCA	2 cut(s)	1223, 2868
BanI	GGYRCC	1 cut(s)	639
BanII	GRGCYC	1 cut(s)	1606
BbsI	GAAGAC	2 cut(s)	774, 1563
Bbv12I	GWGCWC	5 cut(s)	977, 1159, 1213, 1606, 2376
BbvI	GCAGC	3 cut(s)	623, 2180, 2413
BccI	CCATC	5 cut(s)	251, 1088, 2132, 2487, 2788
BceAI	ACGGC	2 cut(s)	81, 2409
BciT130I	CCWGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
BclI	TGATCA	1 cut(s)	703
BcoDI	GTCTC	7 cut(s)	52, 744, 766, 1221, 1938, 2024, 2875
BfaI	CTAG	6 cut(s)	176, 438, 881, 941, 1067, 1496
BfmI	CTRYAG	1 cut(s)	2736
BfuAI	ACCTGC	1 cut(s)	656
BglI	GCCNNNNNGGC	1 cut(s)	201
BisI	GCNGC	3 cut(s)	612, 2169, 2402
BlsI	GCNGC	3 cut(s)	613, 2170, 2403
BmcAI	AGTACT	1 cut(s)	2841
Bme1390I	CCNGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
Bme18I	GGWCC	1 cut(s)	2534
BmeRI	GACNNNNNGTC	1 cut(s)	1930
BmeT110I	CYCGRG	1 cut(s)	139
BmgT120I	GGNCC	4 cut(s)	407, 1991, 2434, 2534
BmiI	GGNNCC	4 cut(s)	205, 641, 1472, 1992
BmrFI	CCNGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
BmrI	ACTGGG	1 cut(s)	638
BmsI	GCATC	8 cut(s)	530, 552, 1137, 1223, 1423, 2008, 2155, 2210
BmuI	ACTGGG	1 cut(s)	638
BoxI	GACNNNNGTC	1 cut(s)	778
BpiI	GAAGAC	2 cut(s)	774, 1563
BplI	GAGNNNNNCTC	2 cut(s)	2699, 2731
BpmI	CTGGAG	5 cut(s)	768, 783, 1659, 2258, 2624
Bpu14I	TTCGAA	1 cut(s)	785
BpuEI	CTTGAG	2 cut(s)	2021, 2360
BsaBI	GATNNNNATC	1 cut(s)	2444
BsaI	GGTCTC	1 cut(s)	744
BsaJI	CCNNGG	8 cut(s)	140, 309, 615, 1179, 1825, 1854, 2367, 2450
BsaWI	WCCGGW	2 cut(s)	242, 579
BsaXI	ACNNNNNCTCC	6 cut(s)	1131, 1161, 2127, 2157, 2190, 2220
Bsc4I	CCNNNNNNNGG	2 cut(s)	1855, 2784
Bse1I	ACTGG	4 cut(s)	644, 958, 1306, 2241
Bse3DI	GCAATG	4 cut(s)	69, 875, 1726, 2259
Bse8I	GATNNNNATC	1 cut(s)	2444
BseAI	TCCGGA	1 cut(s)	242
BseBI	CCWGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
BseDI	CCNNGG	8 cut(s)	140, 309, 615, 1179, 1825, 1854, 2367, 2450
BseGI	GGATG	6 cut(s)	472, 545, 1128, 1435, 2023, 2667
BseJI	GATNNNNATC	1 cut(s)	2444
BseLI	CCNNNNNNNGG	2 cut(s)	1855, 2784
BseMI	GCAATG	4 cut(s)	69, 875, 1726, 2259
BseMII	CTCAG	3 cut(s)	1113, 1875, 1881
BseNI	ACTGG	4 cut(s)	644, 958, 1306, 2241
BseRI	GAGGAG	2 cut(s)	296, 1155
BseSI	GKGCMC	2 cut(s)	644, 1159
BseXI	GCAGC	3 cut(s)	623, 2180, 2413
BseYI	CCCAGC	2 cut(s)	1350, 1759
BsgI	GTGCAG	2 cut(s)	684, 2527
Bsh1236I	CGCG	1 cut(s)	231
BshNI	GGYRCC	1 cut(s)	639
BsiHKAI	GWGCWC	5 cut(s)	977, 1159, 1213, 1606, 2376
BsiHKCI	CYCGRG	1 cut(s)	139
BsiSI	CCGG	3 cut(s)	243, 580, 2265
BslFI	GGGAC	2 cut(s)	1740, 2468
BslI	CCNNNNNNNGG	2 cut(s)	1855, 2784
BsmAI	GTCTC	7 cut(s)	52, 744, 766, 1221, 1938, 2024, 2875
BsmFI	GGGAC	2 cut(s)	1740, 2468
Bso31I	GGTCTC	1 cut(s)	744
BsoBI	CYCGRG	1 cut(s)	139
Bsp119I	TTCGAA	1 cut(s)	785
Bsp1286I	GDGCHC	6 cut(s)	644, 977, 1159, 1213, 1606, 2376
Bsp13I	TCCGGA	1 cut(s)	242
Bsp143I	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
Bsp19I	CCATGG	2 cut(s)	615, 2450
BspACI	CCGC	2 cut(s)	231, 1882
BspCNI	CTCAG	3 cut(s)	1114, 1874, 1882
BspEI	TCCGGA	1 cut(s)	242
BspFNI	CGCG	1 cut(s)	231
BspHI	TCATGA	1 cut(s)	2026
BspLI	GGNNCC	4 cut(s)	205, 641, 1472, 1992
BspMI	ACCTGC	1 cut(s)	656
BspPI	GGATC	5 cut(s)	345, 940, 964, 2616, 2671
BspT104I	TTCGAA	1 cut(s)	785
BspT107I	GGYRCC	1 cut(s)	639
BspTNI	GGTCTC	1 cut(s)	744
BsrDI	GCAATG	4 cut(s)	69, 875, 1726, 2259
BsrI	ACTGG	4 cut(s)	644, 958, 1306, 2241
BssECI	CCNNGG	8 cut(s)	140, 309, 615, 1179, 1825, 1854, 2367, 2450
BssMI	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
BssT1I	CCWWGG	4 cut(s)	309, 615, 1854, 2450
Bst2UI	CCWGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
Bst6I	CTCTTC	1 cut(s)	19
BstAPI	GCANNNNNTGC	3 cut(s)	2098, 2165, 2207
BstBI	TTCGAA	1 cut(s)	785
BstC8I	GCNNGC	3 cut(s)	233, 1872, 2157
BstDEI	CTNAG	6 cut(s)	960, 1122, 1730, 1861, 1890, 2053
BstDSI	CCRYGG	2 cut(s)	615, 2450
BstEII	GGTNACC	1 cut(s)	2111
BstF5I	GGATG	6 cut(s)	472, 545, 1128, 1435, 2023, 2667
BstFNI	CGCG	1 cut(s)	231
BstHHI	GCGC	3 cut(s)	231, 316, 1785
BstKTI	GATC	9 cut(s)	353, 495, 706, 935, 959, 1117, 1414, 2611, 2679
BstMAI	GTCTC	7 cut(s)	52, 744, 766, 1221, 1938, 2024, 2875
BstMBI	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
BstMWI	GCNNNNNNNGC	9 cut(s)	201, 1208, 1442, 1765, 1871, 2098, 2165, 2207, 2653
BstNI	CCWGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
BstNSI	RCATGY	1 cut(s)	1451
BstPAI	GACNNNNGTC	1 cut(s)	778
BstPI	GGTNACC	1 cut(s)	2111
BstSCI	CCNGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
BstSFI	CTRYAG	1 cut(s)	2736
BstSLI	GKGCMC	2 cut(s)	644, 1159
BstUI	CGCG	1 cut(s)	231
BstV1I	GCAGC	3 cut(s)	623, 2180, 2413
BstV2I	GAAGAC	2 cut(s)	774, 1563
BstX2I	RGATCY	3 cut(s)	932, 956, 2608
BstXI	CCANNNNNNTGG	2 cut(s)	2123, 2493
BstYI	RGATCY	3 cut(s)	932, 956, 2608
BtgI	CCRYGG	2 cut(s)	615, 2450
BtsCI	GGATG	6 cut(s)	472, 545, 1128, 1435, 2023, 2667
BtsI	GCAGTG	2 cut(s)	934, 2625
BtsIMutI	CAGTG	6 cut(s)	934, 951, 1657, 1928, 2625, 2813
BveI	ACCTGC	1 cut(s)	656
Cac8I	GCNNGC	3 cut(s)	233, 1872, 2157
CciI	TCATGA	1 cut(s)	2026
CfoI	GCGC	3 cut(s)	231, 316, 1785
Cfr13I	GGNCC	4 cut(s)	407, 1991, 2434, 2534
Csp6I	GTAC	7 cut(s)	514, 695, 1305, 2255, 2308, 2682, 2840
CspCI	CAANNNNNGTGG	4 cut(s)	1859, 1894, 2612, 2647
CviQI	GTAC	7 cut(s)	514, 695, 1305, 2255, 2308, 2682, 2840
DdeI	CTNAG	6 cut(s)	960, 1122, 1730, 1861, 1890, 2053
DpnI	GATC	9 cut(s)	352, 494, 705, 934, 958, 1116, 1413, 2610, 2678
DpnII	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
DriI	GACNNNNNGTC	1 cut(s)	1930
EaeI	YGGCCR	4 cut(s)	66, 1221, 2278, 2866
Eam1104I	CTCTTC	1 cut(s)	19
Eam1105I	GACNNNNNGTC	1 cut(s)	1930
EarI	CTCTTC	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	1604
Eco130I	CCWWGG	4 cut(s)	309, 615, 1854, 2450
Eco147I	AGGCCT	1 cut(s)	809
Eco24I	GRGCYC	1 cut(s)	1606
Eco31I	GGTCTC	1 cut(s)	744
Eco32I	GATATC	2 cut(s)	853, 1282
Eco47I	GGWCC	1 cut(s)	2534
Eco53kI	GAGCTC	1 cut(s)	1604
Eco57I	CTGAAG	1 cut(s)	399
Eco88I	CYCGRG	1 cut(s)	139
Eco91I	GGTNACC	1 cut(s)	2111
EcoICRI	GAGCTC	1 cut(s)	1604
EcoO65I	GGTNACC	1 cut(s)	2111
EcoRII	CCWGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
EcoRV	GATATC	2 cut(s)	853, 1282
EcoT14I	CCWWGG	4 cut(s)	309, 615, 1854, 2450
EcoT22I	ATGCAT	3 cut(s)	545, 1238, 1292
EcoT38I	GRGCYC	1 cut(s)	1606
ErhI	CCWWGG	4 cut(s)	309, 615, 1854, 2450
FalI	AAGNNNNNCTT	2 cut(s)	1197, 1229
FaqI	GGGAC	2 cut(s)	1740, 2468
FauI	CCCGC	1 cut(s)	224
FbaI	TGATCA	1 cut(s)	703
FblI	GTMKAC	1 cut(s)	180
Fnu4HI	GCNGC	3 cut(s)	612, 2169, 2402
FokI	GGATG	6 cut(s)	459, 552, 1115, 1442, 2030, 2654
FriOI	GRGCYC	1 cut(s)	1606
Fsp4HI	GCNGC	3 cut(s)	612, 2169, 2402
FspBI	CTAG	6 cut(s)	176, 438, 881, 941, 1067, 1496
GlaI	GCGC	3 cut(s)	230, 315, 1784
GluI	GCNGC	3 cut(s)	612, 2169, 2402
GsaI	CCCAGC	2 cut(s)	1354, 1763
GsuI	CTGGAG	5 cut(s)	768, 783, 1659, 2258, 2624
HapII	CCGG	3 cut(s)	243, 580, 2265
HhaI	GCGC	3 cut(s)	231, 316, 1785
Hin6I	GCGC	3 cut(s)	229, 314, 1783
HinP1I	GCGC	3 cut(s)	229, 314, 1783
HincII	GTYRAC	2 cut(s)	1189, 1648
HindII	GTYRAC	2 cut(s)	1189, 1648
HindIII	AAGCTT	2 cut(s)	287, 1246
HpaI	GTTAAC	1 cut(s)	1189
HpaII	CCGG	3 cut(s)	243, 580, 2265
HphI	GGTGA	8 cut(s)	737, 1069, 1303, 1603, 1656, 1934, 2056, 2767
Hpy166II	GTNNAC	5 cut(s)	181, 1157, 1189, 1307, 1648
Hpy8I	GTNNAC	5 cut(s)	181, 1157, 1189, 1307, 1648
Hpy99I	CGWCG	2 cut(s)	1038, 2518
HpyAV	CCTTC	4 cut(s)	391, 1521, 1888, 2636
HpyCH4IV	ACGT	1 cut(s)	2516
HpyF10VI	GCNNNNNNNGC	9 cut(s)	201, 1208, 1442, 1765, 1871, 2098, 2165, 2207, 2653
HpyF3I	CTNAG	6 cut(s)	960, 1122, 1730, 1861, 1890, 2053
HpySE526I	ACGT	1 cut(s)	2516
HspAI	GCGC	3 cut(s)	229, 314, 1783
Kpn2I	TCCGGA	1 cut(s)	242
Ksp22I	TGATCA	1 cut(s)	703
KspAI	GTTAAC	1 cut(s)	1189
Kzo9I	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
LmnI	GCTCC	4 cut(s)	209, 1208, 1601, 2198
Lsp1109I	GCAGC	3 cut(s)	623, 2180, 2413
LweI	GCATC	8 cut(s)	530, 552, 1137, 1223, 1423, 2008, 2155, 2210
MaeI	CTAG	6 cut(s)	176, 438, 881, 941, 1067, 1496
MaeII	ACGT	1 cut(s)	2516
MaeIII	GTNAC	6 cut(s)	1075, 1591, 1922, 1984, 2111, 2557
MalI	GATC	9 cut(s)	352, 494, 705, 934, 958, 1116, 1413, 2610, 2678
MboI	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
MfeI	CAATTG	2 cut(s)	2106, 2754
MflI	RGATCY	3 cut(s)	932, 956, 2608
MhlI	GDGCHC	6 cut(s)	644, 977, 1159, 1213, 1606, 2376
MlsI	TGGCCA	2 cut(s)	1223, 2868
MluNI	TGGCCA	2 cut(s)	1223, 2868
MlyI	GAGTC	8 cut(s)	833, 1593, 1853, 1919, 2055, 2144, 2277, 2877
MmeI	TCCRAC	3 cut(s)	231, 1498, 2104
Mox20I	TGGCCA	2 cut(s)	1223, 2868
Mph1103I	ATGCAT	3 cut(s)	545, 1238, 1292
MroI	TCCGGA	1 cut(s)	242
MroXI	GAANNNNTTC	1 cut(s)	2646
MscI	TGGCCA	2 cut(s)	1223, 2868
MseI	TTAA	7 cut(s)	480, 815, 887, 1188, 1335, 1916, 2037
MslI	CAYNNNNRTG	2 cut(s)	2315, 2491
Msp20I	TGGCCA	2 cut(s)	1223, 2868
MspA1I	CMGCKG	2 cut(s)	848, 1350
MspI	CCGG	3 cut(s)	243, 580, 2265
MspR9I	CCNGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
MunI	CAATTG	2 cut(s)	2106, 2754
MvaI	CCWGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
MvnI	CGCG	1 cut(s)	231
MwoI	GCNNNNNNNGC	9 cut(s)	201, 1208, 1442, 1765, 1871, 2098, 2165, 2207, 2653
NcoI	CCATGG	2 cut(s)	615, 2450
NdeII	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
NlaIV	GGNNCC	4 cut(s)	205, 641, 1472, 1992
NmeAIII	GCCGAG	1 cut(s)	1806
NmuCI	GTSAC	3 cut(s)	1075, 1591, 1922
NsiI	ATGCAT	3 cut(s)	545, 1238, 1292
NspI	RCATGY	1 cut(s)	1451
NspV	TTCGAA	1 cut(s)	785
PagI	TCATGA	1 cut(s)	2026
PceI	AGGCCT	1 cut(s)	809
PciI	ACATGT	1 cut(s)	1447
PdmI	GAANNNNTTC	1 cut(s)	2646
PfeI	GAWTC	8 cut(s)	222, 862, 1318, 1464, 1504, 1808, 2192, 2760
PfoI	TCCNGGA	2 cut(s)	760, 827
PkrI	GCNGC	3 cut(s)	613, 2170, 2403
PleI	GAGTC	8 cut(s)	832, 1592, 1853, 1919, 2055, 2144, 2277, 2877
PpsI	GAGTC	8 cut(s)	832, 1592, 1853, 1919, 2055, 2144, 2277, 2877
PscI	ACATGT	1 cut(s)	1447
PshAI	GACNNNNGTC	1 cut(s)	778
PsiI	TTATAA	4 cut(s)	1091, 1697, 2589, 2901
Psp124BI	GAGCTC	1 cut(s)	1606
Psp6I	CCWGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
PspEI	GGTNACC	1 cut(s)	2111
PspFI	CCCAGC	2 cut(s)	1350, 1759
PspGI	CCWGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
PspN4I	GGNNCC	4 cut(s)	205, 641, 1472, 1992
PspPI	GGNCC	4 cut(s)	407, 1991, 2434, 2534
PsuI	RGATCY	3 cut(s)	932, 956, 2608
PvuII	CAGCTG	2 cut(s)	848, 1350
RsaI	GTAC	7 cut(s)	515, 696, 1306, 2256, 2309, 2683, 2841
RsaNI	GTAC	7 cut(s)	514, 695, 1305, 2255, 2308, 2682, 2840
RseI	CAYNNNNRTG	2 cut(s)	2315, 2491
SacI	GAGCTC	1 cut(s)	1606
SaqAI	TTAA	7 cut(s)	480, 815, 887, 1188, 1335, 1916, 2037
SatI	GCNGC	3 cut(s)	612, 2169, 2402
Sau3AI	GATC	9 cut(s)	350, 492, 703, 932, 956, 1114, 1411, 2608, 2676
Sau96I	GGNCC	4 cut(s)	407, 1991, 2434, 2534
ScaI	AGTACT	1 cut(s)	2841
SchI	GAGTC	8 cut(s)	833, 1593, 1853, 1919, 2055, 2144, 2277, 2877
ScrFI	CCNGG	9 cut(s)	723, 747, 762, 829, 1181, 2015, 2369, 2870, 2908
SduI	GDGCHC	6 cut(s)	644, 977, 1159, 1213, 1606, 2376
SfaNI	GCATC	8 cut(s)	530, 552, 1137, 1223, 1423, 2008, 2155, 2210
SfcI	CTRYAG	1 cut(s)	2736
SfuI	TTCGAA	1 cut(s)	785
SinI	GGWCC	1 cut(s)	2534
SmiMI	CAYNNNNRTG	2 cut(s)	2315, 2491
SmlI	CTYRAG	2 cut(s)	2000, 2375
SmoI	CTYRAG	2 cut(s)	2000, 2375
SseBI	AGGCCT	1 cut(s)	809
SsiI	CCGC	2 cut(s)	231, 1882
SspI	AATATT	1 cut(s)	895
SspMI	CTAG	6 cut(s)	176, 438, 881, 941, 1067, 1496
SstI	GAGCTC	1 cut(s)	1606
StuI	AGGCCT	1 cut(s)	809
StyD4I	CCNGG	9 cut(s)	721, 745, 760, 827, 1179, 2013, 2367, 2868, 2906
StyI	CCWWGG	4 cut(s)	309, 615, 1854, 2450
TaiI	ACGT	1 cut(s)	2519
TaqI	TCGA	8 cut(s)	33, 266, 339, 349, 785, 2059, 2163, 2640
TaqII	GACCGA	2 cut(s)	1424, 1721
TatI	WGTACW	4 cut(s)	694, 2254, 2307, 2839
TfiI	GAWTC	8 cut(s)	222, 862, 1318, 1464, 1504, 1808, 2192, 2760
Tru1I	TTAA	7 cut(s)	480, 815, 887, 1188, 1335, 1916, 2037
Tru9I	TTAA	7 cut(s)	480, 815, 887, 1188, 1335, 1916, 2037
TscAI	CASTG	6 cut(s)	934, 958, 1657, 1935, 2632, 2820
TseFI	GTSAC	3 cut(s)	1075, 1591, 1922
TseI	GCWGC	3 cut(s)	611, 2168, 2401
Tsp45I	GTSAC	3 cut(s)	1075, 1591, 1922
TspGWI	ACGGA	2 cut(s)	612, 2669
TspRI	CASTG	6 cut(s)	934, 958, 1657, 1935, 2632, 2820
VneI	GTGCAC	1 cut(s)	1155
VpaK11BI	GGWCC	1 cut(s)	2534
XapI	RAATTY	4 cut(s)	99, 629, 1016, 2691
XceI	RCATGY	1 cut(s)	1451
XmiI	GTMKAC	1 cut(s)	180
XmnI	GAANNNNTTC	1 cut(s)	2646
XspI	CTAG	6 cut(s)	176, 438, 881, 941, 1067, 1496
ZrmI	AGTACT	1 cut(s)	2841
Zsp2I	ATGCAT	3 cut(s)	545, 1238, 1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.