Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Forward (+)

32726180 .. 32730764

Gene Structure 4585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1317200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGAGATGGTTCAAAACCCCTAGCATTTTCAGTTCAAATAATCACAACCTTCCTTACTACAACCCGATTCGCGAAAACCTCGTAAACAACTCTGAAACGCCGAAAGATGGTGTCCCCAACAAGCTCTTTCGACTACTTCGCAGGCTTATGTCTGGAGGAAAAATTGATGGGGGATCAAGGACTGAGGCAGAGGAGAAGCTCTACTCTTGGTTATACGCCTTGGCGCAGTCCGACAAGGATTTGGTTTTCGAGTATGTTCGATCCACTGAAAGGGGATTGAGCTTTACTGAAGCTGAGCGGAGATTGAAGGAAAATGGCCCGAATGTTCCTGTGGATTATTCTTTTCTGAGCTGGTGGCGTATTTTGTGGAATGCTTTCTTTCATCCTTTTAATATCATATTGATCGTCTTGTCTGTAATCTCGTACATAACCAGTGATAGCCCGAATGGATGTATCATGCTTGTTTTGGTCTTCATAAGTGTCTCCCTTCGATTCTATCAGGAATATGGCAGTTCAAAAGCGGCCATGAAACTCTCAGAATTCGTAAGGTGCCCGGTCAAAGTTCAAAGATGTGCAGGTAGAGTTGTTCAGACCGAATTAGTTGTACAAATTGATCAAAGAGATGTCGTTCCTGGCGACATTGTCATTTTCGAACCTGGAGACCTCTTTCCTGGTGATGTGAGGCTTCTGTCTACGAAACACCTTGTTGTAAGTCAGTCCTCATTAACAGGAGAGTCCTGGACAACTGAGAAAACAGCTGATATCAGAGAAGATTCGAGCACTCCATTGCTAGATTTACGGAATATTTGCTTCATGGGAACAAATGTAGTATCGGGCGGTGGAGCTGGTCTAGTGGTGTCCACCGGATCCAAGACGTACATGAGCACCATGTTTTCAAACATAGGAAAGAAGAAACCACCAAATGAATTTGAGGATGGTGTCCGCAAAATATCTTACGTTCTGGTTGCTGTTATGCTTGTAGTAGTCACCATCATAGTTGTAACTGACTACACTACATCTCATGATCTGACTGAGAGCATCCTTTTCGGAGTCTCAGTGGCAAGTGCACTCACTCCTCAAATGCTTCCTCTCATTGTTAACACAAGTCTCGCGAAAGGAGCACTTGCTATGGCCAAAGACAGATGCATAATCAAAAGCTTAACTGCGATACGGGACATGGGATCTATGGATATCTTATGCATTGACAAGACCGGTACGCTCACCATGAACCGTGCAATAATGGTTAATCATCTGGACAGCTGGGGGTTACAGAAGGAAAAGGTTTTACAGTTTGCTTTCCTCAACTCCTATTTCAAGACCGACCAGAAGTATCCCCTTGACGATGCAATTTTGGCACATGTATATACCAATGGATACAAGTTCCAGCCTTCGAAATGGAAGAAAATAGATGAGATTCCTTTTGATTTCATAAGAAGAAGAGTAGCTATTATCATGGAGAGAGAGGCAGAAGACAGAAGCCACCACATTTCCGACAGAATTGTGGTGACAAAAGGAGCTCTGGAAGAAGTGATGAAAGTTTGTTCTTTTATAGAGGATGTTGACGGGGAAACGAATATAACTTTCTCTACAGAACACTATCAGAGGATTTTAAATCTGACAGAAGAAATTAGCTATGAGGGACTCAGAGTTATTGGGGTGGCAATAAAGAGGCTGGGAACAGAAACGAATTATCAATGCAGAGATGATGATGAAACTTTTGAATCAGACATGGTTTTCCTCGGCCTCATAACATTCTTTGACCCACCCAAGGACTCAGCAAAGCAAGCTCTGTGGCGGTTGGCTGAGAAGGGAGTGAAAGCAAAGGTCTTGACAGGTGACTCGCTCGCCCTATCAATTAGAGTATGCAAAGAAGTTGGTATCAGAACCACCCATGTAGTTACAGGGCCAGAGCTTGAGTTACTCGACCAAGATTCCTTTCATGAGACGGTTAAAAGAGCAACTGTACTAGCTCGACTCACCCCAACACAGAAACTCCGAGTTGTGCAGTCCCTGCAGACAGTTGGAAACCACACTGTTGGCTTCTTAGGAGATGGAGTCAATGACTCGCTTGCACTGGATGCAGCCAATGTTGGTATATCAATTGATTCAGGAGTATCAGTTGCAAAAGACTTTGCTGACATTATATTACTGGAGAAGGACCTCAATGTCCTCACCGCTGGAGTTGAGCACGGTCGACTTACTTTTGGGAACACAATGAAGTACATAAAAATGTCAGTAATTGCCAATCTAGGAAGCGTTCTCTCAATTCTCATAGCAACCTTGTGTCTCAAGTATGAACCATTGACTCCACGACAGCTTCTTACGCAGAACTTCTTGTATAGTGTTGGGCAGATCGCAATTCCATGGGACAAAATGGAAGAAGATTATGTAAATGTCCCACAAAAATGGTCGAAGAAAGGCTTACCGATGTTCATTCTGTGGAATGGTCCTGTGTGTACTCTCTTTGACGTAAGCACACTTATGTTCCTTTGGTTCTATTATAAGGCTGACAGTCTGGATGACATTGTGTTCTTCCATTCTGCTTGGTTCATCGAAGGGCTTCTGATGCAGACTCTGATCATCCACTTGATCCGGACAGAGAAAATTCCATTCATTCAGGAGATTGCCTCATGGCCTGTGCTTTGTTCCACGGTTCTGATTTCCGCCATCGGAATTGCTATTCCTTTCACACCAATTGGGGATGTGATGGGATTCGTTGAGCTTCCGCTATCATACTTTGGATTTTTGGTGGTGCTTTTTGTAGGATATATTTTCGTCGGCCAGGTGGTCAAGAGACTATATATTATGGTTTACAAAAGCTGGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

943

Amino Acids

105.89

Weight (kDa)

6.09

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	79 - 141	7.2e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	194 - 298	3.7e-17	P-type ATPase actuator domain
Hydrolase	PF00702	398 - 695	5.9e-08	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	449 - 591	1.1e-20	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	767 - 933	2e-17	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2502
AasI	GACNNNNNNGTC	1 cut(s)	2165
Acc36I	ACCTGC	1 cut(s)	566
AccB1I	GGYRCC	1 cut(s)	549
AccBSI	CCGCTC	1 cut(s)	298
AccI	GTMKAC	2 cut(s)	692, 2194
AccII	CGCG	2 cut(s)	72, 1112
AccIII	TCCGGA	1 cut(s)	2592
AciI	CCGC	8 cut(s)	298, 521, 837, 943, 1795, 2175, 2664, 2726
AclWI	GGATC	6 cut(s)	181, 255, 861, 874, 1189, 2584
AcoI	YGGCCR	3 cut(s)	522, 1131, 2779
AcsI	RAATTY	3 cut(s)	539, 926, 2604
AcuI	CTGAAG	1 cut(s)	309
AfaI	GTAC	7 cut(s)	425, 606, 878, 1216, 1965, 2222, 2458
AfiI	CCNNNNNNNGG	3 cut(s)	107, 270, 1768
AflIII	ACRYGT	1 cut(s)	1357
AgeI	ACCGGT	1 cut(s)	1211
AgsI	TTSAA	8 cut(s)	13, 36, 307, 516, 566, 897, 1315, 1721
AjnI	CCWGG	5 cut(s)	631, 655, 670, 737, 2781
AjuI	GAANNNNNNNTTGG	2 cut(s)	1920, 1952
Alw21I	GWGCWC	6 cut(s)	782, 887, 1069, 1123, 1519, 2190
Alw26I	GTCTC	7 cut(s)	487, 654, 1057, 1112, 1937, 2291, 2788
Alw44I	GTGCAC	1 cut(s)	1065
AlwI	GGATC	6 cut(s)	181, 255, 861, 874, 1189, 2584
AlwNI	CAGNNNCTG	2 cut(s)	2011, 2575
Aor13HI	TCCGGA	1 cut(s)	2592
AoxI	GGCC	7 cut(s)	316, 522, 1131, 1741, 1904, 2633, 2779
ApaLI	GTGCAC	1 cut(s)	1065
ApeKI	GCWGC	1 cut(s)	2081
ApoI	RAATTY	3 cut(s)	539, 926, 2604
AsiGI	ACCGGT	1 cut(s)	1211
Asp700I	GAANNNNTTC	2 cut(s)	374, 2559
AspLEI	GCGC	1 cut(s)	226
AspS9I	GGNCC	4 cut(s)	317, 1904, 2158, 2447
AsuC2I	CCSGG	1 cut(s)	554
AsuHPI	GGTGA	7 cut(s)	686, 979, 1213, 1516, 1847, 1969, 2164
AsuII	TTCGAA	2 cut(s)	651, 1391
AvaII	GGWCC	2 cut(s)	2158, 2447
BaeGI	GKGCMC	2 cut(s)	554, 1069
BalI	TGGCCA	1 cut(s)	1133
BamHI	GGATCC	1 cut(s)	866
BanI	GGYRCC	1 cut(s)	549
BanII	GRGCYC	1 cut(s)	1519
BbsI	GAAGAC	2 cut(s)	463, 1476
Bbv12I	GWGCWC	6 cut(s)	782, 887, 1069, 1123, 1519, 2190
BbvI	GCAGC	1 cut(s)	2093
BccI	CCATC	7 cut(s)	101, 161, 929, 998, 2045, 2675, 2701
BcgI	CGANNNNNNTGC	2 cut(s)	1027, 1061
BciT130I	CCWGG	5 cut(s)	633, 657, 672, 739, 2783
BciVI	GTATCC	2 cut(s)	1341, 1367
BclI	TGATCA	2 cut(s)	613, 2577
BcnI	CCSGG	1 cut(s)	554
BcoDI	GTCTC	7 cut(s)	487, 654, 1057, 1112, 1937, 2291, 2788
BfaI	CTAG	5 cut(s)	21, 791, 851, 1967, 2249
BfmI	CTRYAG	2 cut(s)	1587, 2012
BfuAI	ACCTGC	1 cut(s)	566
BfuI	GTATCC	2 cut(s)	1341, 1367
BisI	GCNGC	2 cut(s)	522, 2082
BlpI	GCTNAGC	1 cut(s)	294
BlsI	GCNGC	2 cut(s)	523, 2083
Bme1390I	CCNGG	6 cut(s)	554, 633, 657, 672, 739, 2783
Bme18I	GGWCC	2 cut(s)	2158, 2447
BmgT120I	GGNCC	4 cut(s)	317, 1904, 2158, 2447
BmiI	GGNNCC	2 cut(s)	551, 868
BmrFI	CCNGG	6 cut(s)	554, 633, 657, 672, 739, 2783
BmsI	GCATC	5 cut(s)	1047, 1133, 1333, 2068, 2556
BpiI	GAAGAC	2 cut(s)	463, 1476
BplI	GAGNNNNNCTC	2 cut(s)	2612, 2644
BpmI	CTGGAG	4 cut(s)	174, 678, 2171, 2199
Bpu1102I	GCTNAGC	1 cut(s)	294
Bpu14I	TTCGAA	2 cut(s)	651, 1391
BpuEI	CTTGAG	2 cut(s)	1934, 2273
BpuMI	CCSGG	1 cut(s)	554
BsaI	GGTCTC	1 cut(s)	654
BsaJI	CCNNGG	5 cut(s)	219, 1738, 1767, 2363, 2649
BsaWI	WCCGGW	3 cut(s)	863, 1211, 2592
BsaXI	ACNNNNNCTCC	8 cut(s)	1041, 1071, 1977, 2007, 2040, 2070, 2103, 2133
Bsc4I	CCNNNNNNNGG	3 cut(s)	107, 270, 1768
Bse118I	RCCGGY	1 cut(s)	1211
Bse1I	ACTGG	3 cut(s)	432, 2079, 2154
Bse3DI	GCAATG	1 cut(s)	785
BseAI	TCCGGA	1 cut(s)	2592
BseBI	CCWGG	5 cut(s)	633, 657, 672, 739, 2783
BseDI	CCNNGG	5 cut(s)	219, 1738, 1767, 2363, 2649
BseGI	GGATG	9 cut(s)	382, 455, 940, 1038, 1561, 2083, 2524, 2580, 2707
BseLI	CCNNNNNNNGG	3 cut(s)	107, 270, 1768
BseMI	GCAATG	1 cut(s)	785
BseNI	ACTGG	3 cut(s)	432, 2079, 2154
BseRI	GAGGAG	2 cut(s)	206, 1065
BseSI	GKGCMC	2 cut(s)	554, 1069
BseXI	GCAGC	1 cut(s)	2093
BseYI	CCCAGC	2 cut(s)	1260, 1672
BsgI	GTGCAG	2 cut(s)	594, 2024
Bsh1236I	CGCG	2 cut(s)	72, 1112
Bsh1285I	CGRYCG	1 cut(s)	2194
BshFI	GGCC	7 cut(s)	318, 524, 1133, 1743, 1906, 2635, 2781
BshNI	GGYRCC	1 cut(s)	549
BshTI	ACCGGT	1 cut(s)	1211
BsiEI	CGRYCG	1 cut(s)	2194
BsiHKAI	GWGCWC	6 cut(s)	782, 887, 1069, 1123, 1519, 2190
BsiSI	CCGG	4 cut(s)	554, 864, 1212, 2593
BslFI	GGGAC	6 cut(s)	98, 1187, 1653, 1993, 2381, 2381
BslI	CCNNNNNNNGG	3 cut(s)	107, 270, 1768
BsmAI	GTCTC	7 cut(s)	487, 654, 1057, 1112, 1937, 2291, 2788
BsmBI	CGTCTC	1 cut(s)	1937
BsmFI	GGGAC	6 cut(s)	98, 1187, 1653, 1993, 2381, 2381
BsmI	GAATGC	1 cut(s)	376
BsnI	GGCC	7 cut(s)	318, 524, 1133, 1743, 1906, 2635, 2781
Bso31I	GGTCTC	1 cut(s)	654
Bsp119I	TTCGAA	2 cut(s)	651, 1391
Bsp1286I	GDGCHC	7 cut(s)	554, 782, 887, 1069, 1123, 1519, 2190
Bsp13I	TCCGGA	1 cut(s)	2592
Bsp1407I	TGTACA	1 cut(s)	604
Bsp1720I	GCTNAGC	1 cut(s)	294
Bsp19I	CCATGG	1 cut(s)	2363
Bsp68I	TCGCGA	2 cut(s)	72, 1112
BspACI	CCGC	8 cut(s)	298, 521, 837, 943, 1795, 2175, 2664, 2726
BspANI	GGCC	7 cut(s)	318, 524, 1133, 1743, 1906, 2635, 2781
BspEI	TCCGGA	1 cut(s)	2592
BspFNI	CGCG	2 cut(s)	72, 1112
BspHI	TCATGA	2 cut(s)	1021, 1939
BspLI	GGNNCC	2 cut(s)	551, 868
BspMAI	CTGCAG	1 cut(s)	2016
BspMI	ACCTGC	1 cut(s)	566
BspPI	GGATC	6 cut(s)	181, 255, 861, 874, 1189, 2584
BspT104I	TTCGAA	2 cut(s)	651, 1391
BspT107I	GGYRCC	1 cut(s)	549
BspTNI	GGTCTC	1 cut(s)	654
BsrBI	CCGCTC	1 cut(s)	298
BsrDI	GCAATG	1 cut(s)	785
BsrFI	RCCGGY	1 cut(s)	1211
BsrGI	TGTACA	1 cut(s)	604
BsrI	ACTGG	3 cut(s)	432, 2079, 2154
BssAI	RCCGGY	1 cut(s)	1211
BssECI	CCNNGG	5 cut(s)	219, 1738, 1767, 2363, 2649
BssT1I	CCWWGG	3 cut(s)	219, 1767, 2363
Bst2UI	CCWGG	5 cut(s)	633, 657, 672, 739, 2783
Bst4CI	ACNGT	9 cut(s)	1232, 1290, 1948, 1963, 2020, 2035, 2192, 2513, 2653
Bst6I	CTCTTC	1 cut(s)	1432
BstAPI	GCANNNNNTGC	2 cut(s)	2011, 2078
BstAUI	TGTACA	1 cut(s)	604
BstBI	TTCGAA	2 cut(s)	651, 1391
BstC8I	GCNNGC	5 cut(s)	143, 1785, 1845, 2070, 2822
BstDSI	CCRYGG	2 cut(s)	2363, 2649
BstF5I	GGATG	9 cut(s)	382, 455, 940, 1038, 1561, 2083, 2524, 2580, 2707
BstFNI	CGCG	2 cut(s)	72, 1112
BstHHI	GCGC	1 cut(s)	226
BstMAI	GTCTC	7 cut(s)	487, 654, 1057, 1112, 1937, 2291, 2788
BstMCI	CGRYCG	1 cut(s)	2194
BstMWI	GCNNNNNNNGC	7 cut(s)	1118, 1352, 1784, 2011, 2078, 2323, 2566
BstNI	CCWGG	5 cut(s)	633, 657, 672, 739, 2783
BstNSI	RCATGY	1 cut(s)	1361
BstSCI	CCNGG	6 cut(s)	552, 631, 655, 670, 737, 2781
BstSFI	CTRYAG	2 cut(s)	1587, 2012
BstSLI	GKGCMC	2 cut(s)	554, 1069
BstUI	CGCG	2 cut(s)	72, 1112
BstV1I	GCAGC	1 cut(s)	2093
BstV2I	GAAGAC	2 cut(s)	463, 1476
BstX2I	RGATCY	2 cut(s)	866, 1181
BstXI	CCANNNNNNTGG	2 cut(s)	2036, 2406
BstYI	RGATCY	2 cut(s)	866, 1181
BsuI	GTATCC	2 cut(s)	1341, 1367
BsuRI	GGCC	7 cut(s)	318, 524, 1133, 1743, 1906, 2635, 2781
BtgI	CCRYGG	2 cut(s)	2363, 2649
BtsCI	GGATG	9 cut(s)	382, 455, 940, 1038, 1561, 2083, 2524, 2580, 2707
BtsIMutI	CAGTG	5 cut(s)	264, 439, 1062, 2031, 2072
BtuMI	TCGCGA	2 cut(s)	72, 1112
BveI	ACCTGC	1 cut(s)	566
Cac8I	GCNNGC	5 cut(s)	143, 1785, 1845, 2070, 2822
CaiI	CAGNNNCTG	2 cut(s)	2011, 2575
CciI	TCATGA	2 cut(s)	1021, 1939
CfoI	GCGC	1 cut(s)	226
Cfr10I	RCCGGY	1 cut(s)	1211
Cfr13I	GGNCC	4 cut(s)	317, 1904, 2158, 2447
Csp6I	GTAC	7 cut(s)	424, 605, 877, 1215, 1964, 2221, 2457
CspAI	ACCGGT	1 cut(s)	1211
CspCI	CAANNNNNGTGG	2 cut(s)	1772, 1807
CviQI	GTAC	7 cut(s)	424, 605, 877, 1215, 1964, 2221, 2457
DraI	TTTAAA	1 cut(s)	1611
DrdI	GACNNNNNNGTC	1 cut(s)	2165
DseDI	GACNNNNNNGTC	1 cut(s)	2165
EaeI	YGGCCR	3 cut(s)	522, 1131, 2779
Eam1104I	CTCTTC	1 cut(s)	1432
EarI	CTCTTC	1 cut(s)	1432
EciI	GGCGGA	1 cut(s)	2653
Ecl136II	GAGCTC	1 cut(s)	1517
Eco130I	CCWWGG	3 cut(s)	219, 1767, 2363
Eco24I	GRGCYC	1 cut(s)	1519
Eco31I	GGTCTC	1 cut(s)	654
Eco32I	GATATC	2 cut(s)	763, 1192
Eco47I	GGWCC	2 cut(s)	2158, 2447
Eco53kI	GAGCTC	1 cut(s)	1517
Eco57I	CTGAAG	1 cut(s)	309
EcoICRI	GAGCTC	1 cut(s)	1517
EcoO109I	RGGNCCY	1 cut(s)	2158
EcoRI	GAATTC	1 cut(s)	539
EcoRII	CCWGG	5 cut(s)	631, 655, 670, 737, 2781
EcoRV	GATATC	2 cut(s)	763, 1192
EcoT14I	CCWWGG	3 cut(s)	219, 1767, 2363
EcoT22I	ATGCAT	2 cut(s)	1148, 1202
EcoT38I	GRGCYC	1 cut(s)	1519
ErhI	CCWWGG	3 cut(s)	219, 1767, 2363
Esp3I	CGTCTC	1 cut(s)	1937
FalI	AAGNNNNNCTT	4 cut(s)	1107, 1139, 2405, 2437
FaqI	GGGAC	6 cut(s)	98, 1187, 1653, 1993, 2381, 2381
FbaI	TGATCA	2 cut(s)	613, 2577
FblI	GTMKAC	2 cut(s)	692, 2194
Fnu4HI	GCNGC	2 cut(s)	522, 2082
FokI	GGATG	9 cut(s)	369, 462, 947, 1025, 1568, 2090, 2531, 2567, 2714
FriOI	GRGCYC	1 cut(s)	1519
Fsp4HI	GCNGC	2 cut(s)	522, 2082
FspBI	CTAG	5 cut(s)	21, 791, 851, 1967, 2249
GlaI	GCGC	1 cut(s)	225
GluI	GCNGC	2 cut(s)	522, 2082
GsaI	CCCAGC	2 cut(s)	1264, 1676
GsuI	CTGGAG	4 cut(s)	174, 678, 2171, 2199
HaeIII	GGCC	7 cut(s)	318, 524, 1133, 1743, 1906, 2635, 2781
HapII	CCGG	4 cut(s)	554, 864, 1212, 2593
HhaI	GCGC	1 cut(s)	226
Hin6I	GCGC	1 cut(s)	224
HinP1I	GCGC	1 cut(s)	224
HincII	GTYRAC	3 cut(s)	1099, 1561, 2195
HindII	GTYRAC	3 cut(s)	1099, 1561, 2195
HindIII	AAGCTT	1 cut(s)	1156
HpaI	GTTAAC	1 cut(s)	1099
HpaII	CCGG	4 cut(s)	554, 864, 1212, 2593
HphI	GGTGA	7 cut(s)	686, 979, 1213, 1516, 1847, 1969, 2164
Hpy166II	GTNNAC	9 cut(s)	85, 693, 861, 1067, 1099, 1561, 2195, 2457, 2812
Hpy8I	GTNNAC	9 cut(s)	85, 693, 861, 1067, 1099, 1561, 2195, 2457, 2812
Hpy99I	CGWCG	1 cut(s)	2780
HpyAV	CCTTC	8 cut(s)	59, 301, 497, 1267, 1398, 1801, 2149, 2549
HpyCH4III	ACNGT	9 cut(s)	1232, 1290, 1948, 1963, 2020, 2035, 2192, 2513, 2653
HpyCH4IV	ACGT	3 cut(s)	875, 957, 2469
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1118, 1352, 1784, 2011, 2078, 2323, 2566
HpySE526I	ACGT	3 cut(s)	875, 957, 2469
HspAI	GCGC	1 cut(s)	224
Kpn2I	TCCGGA	1 cut(s)	2592
Ksp22I	TGATCA	2 cut(s)	613, 2577
KspAI	GTTAAC	1 cut(s)	1099
LmnI	GCTCC	3 cut(s)	842, 1118, 1514
Lsp1109I	GCAGC	1 cut(s)	2093
LweI	GCATC	5 cut(s)	1047, 1133, 1333, 2068, 2556
MaeI	CTAG	5 cut(s)	21, 791, 851, 1967, 2249
MaeII	ACGT	3 cut(s)	875, 957, 2469
MaeIII	GTNAC	7 cut(s)	985, 1000, 1266, 1504, 1835, 1897, 1917
MbiI	CCGCTC	1 cut(s)	298
MfeI	CAATTG	2 cut(s)	2100, 2694
MflI	RGATCY	2 cut(s)	866, 1181
MhlI	GDGCHC	7 cut(s)	554, 782, 887, 1069, 1123, 1519, 2190
MlsI	TGGCCA	1 cut(s)	1133
MluNI	TGGCCA	1 cut(s)	1133
MmeI	TCCRAC	3 cut(s)	255, 1515, 2002
Mox20I	TGGCCA	1 cut(s)	1133
Mph1103I	ATGCAT	2 cut(s)	1148, 1202
MroI	TCCGGA	1 cut(s)	2592
MroXI	GAANNNNTTC	2 cut(s)	374, 2559
MscI	TGGCCA	1 cut(s)	1133
MseI	TTAA	8 cut(s)	390, 725, 1098, 1160, 1245, 1610, 1950, 2827
MslI	CAYNNNNRTG	3 cut(s)	2228, 2404, 2480
Msp20I	TGGCCA	1 cut(s)	1133
MspA1I	CMGCKG	3 cut(s)	758, 1260, 2177
MspI	CCGG	4 cut(s)	554, 864, 1212, 2593
MspR9I	CCNGG	6 cut(s)	554, 633, 657, 672, 739, 2783
MunI	CAATTG	2 cut(s)	2100, 2694
Mva1269I	GAATGC	1 cut(s)	376
MvaI	CCWGG	5 cut(s)	633, 657, 672, 739, 2783
MvnI	CGCG	2 cut(s)	72, 1112
MwoI	GCNNNNNNNGC	7 cut(s)	1118, 1352, 1784, 2011, 2078, 2323, 2566
NciI	CCSGG	1 cut(s)	554
NcoI	CCATGG	1 cut(s)	2363
NlaIV	GGNNCC	2 cut(s)	551, 868
NmeAIII	GCCGAG	1 cut(s)	1719
NmuCI	GTSAC	3 cut(s)	985, 1504, 1835
NruI	TCGCGA	2 cut(s)	72, 1112
NsiI	ATGCAT	2 cut(s)	1148, 1202
NspI	RCATGY	1 cut(s)	1361
NspV	TTCGAA	2 cut(s)	651, 1391
PagI	TCATGA	2 cut(s)	1021, 1939
PciI	ACATGT	1 cut(s)	1357
PcsI	WCGNNNNNNNCGW	1 cut(s)	633
PctI	GAATGC	1 cut(s)	376
PdmI	GAANNNNTTC	2 cut(s)	374, 2559
PfeI	GAWTC	8 cut(s)	67, 492, 773, 1414, 1721, 1931, 2105, 2712
PflFI	GACNNNGTC	1 cut(s)	641
PfoI	TCCNGGA	1 cut(s)	737
PinAI	ACCGGT	1 cut(s)	1211
PkrI	GCNGC	2 cut(s)	523, 2083
PpuMI	RGGWCCY	1 cut(s)	2158
PscI	ACATGT	1 cut(s)	1357
PsiI	TTATAA	1 cut(s)	2502
Psp124BI	GAGCTC	1 cut(s)	1519
Psp5II	RGGWCCY	1 cut(s)	2158
Psp6I	CCWGG	5 cut(s)	631, 655, 670, 737, 2781
PspFI	CCCAGC	2 cut(s)	1260, 1672
PspGI	CCWGG	5 cut(s)	631, 655, 670, 737, 2781
PspN4I	GGNNCC	2 cut(s)	551, 868
PspPI	GGNCC	4 cut(s)	317, 1904, 2158, 2447
PspPPI	RGGWCCY	1 cut(s)	2158
PstI	CTGCAG	1 cut(s)	2016
PstNI	CAGNNNCTG	2 cut(s)	2011, 2575
PsuI	RGATCY	2 cut(s)	866, 1181
PsyI	GACNNNGTC	1 cut(s)	641
PvuII	CAGCTG	2 cut(s)	758, 1260
RruI	TCGCGA	2 cut(s)	72, 1112
RsaI	GTAC	7 cut(s)	425, 606, 878, 1216, 1965, 2222, 2458
RsaNI	GTAC	7 cut(s)	424, 605, 877, 1215, 1964, 2221, 2457
RseI	CAYNNNNRTG	3 cut(s)	2228, 2404, 2480
SacI	GAGCTC	1 cut(s)	1519
SalI	GTCGAC	1 cut(s)	2193
SaqAI	TTAA	8 cut(s)	390, 725, 1098, 1160, 1245, 1610, 1950, 2827
SatI	GCNGC	2 cut(s)	522, 2082
Sau96I	GGNCC	4 cut(s)	317, 1904, 2158, 2447
ScrFI	CCNGG	6 cut(s)	554, 633, 657, 672, 739, 2783
SduI	GDGCHC	7 cut(s)	554, 782, 887, 1069, 1123, 1519, 2190
SfaNI	GCATC	5 cut(s)	1047, 1133, 1333, 2068, 2556
SfcI	CTRYAG	2 cut(s)	1587, 2012
SfuI	TTCGAA	2 cut(s)	651, 1391
SinI	GGWCC	2 cut(s)	2158, 2447
SmiMI	CAYNNNNRTG	3 cut(s)	2228, 2404, 2480
SmlI	CTYRAG	2 cut(s)	1913, 2288
SmoI	CTYRAG	2 cut(s)	1913, 2288
SsiI	CCGC	8 cut(s)	298, 521, 837, 943, 1795, 2175, 2664, 2726
SspI	AATATT	1 cut(s)	805
SspMI	CTAG	5 cut(s)	21, 791, 851, 1967, 2249
SstI	GAGCTC	1 cut(s)	1519
StyD4I	CCNGG	6 cut(s)	552, 631, 655, 670, 737, 2781
StyI	CCWWGG	3 cut(s)	219, 1767, 2363
TaaI	ACNGT	9 cut(s)	1232, 1290, 1948, 1963, 2020, 2035, 2192, 2513, 2653
TaiI	ACGT	3 cut(s)	878, 960, 2472
TaqII	GACCGA	2 cut(s)	608, 1334
TatI	WGTACW	4 cut(s)	604, 1963, 2220, 2456
TauI	GCSGC	1 cut(s)	524
TfiI	GAWTC	8 cut(s)	67, 492, 773, 1414, 1721, 1931, 2105, 2712
Tru1I	TTAA	8 cut(s)	390, 725, 1098, 1160, 1245, 1610, 1950, 2827
Tru9I	TTAA	8 cut(s)	390, 725, 1098, 1160, 1245, 1610, 1950, 2827
TscAI	CASTG	5 cut(s)	271, 439, 1062, 2038, 2079
TseFI	GTSAC	3 cut(s)	985, 1504, 1835
TseI	GCWGC	1 cut(s)	2081
Tsp45I	GTSAC	3 cut(s)	985, 1504, 1835
TspGWI	ACGGA	1 cut(s)	814
TspRI	CASTG	5 cut(s)	271, 439, 1062, 2038, 2079
Tth111I	GACNNNGTC	1 cut(s)	641
VneI	GTGCAC	1 cut(s)	1065
VpaK11BI	GGWCC	2 cut(s)	2158, 2447
XapI	RAATTY	3 cut(s)	539, 926, 2604
XceI	RCATGY	1 cut(s)	1361
XmiI	GTMKAC	2 cut(s)	692, 2194
XmnI	GAANNNNTTC	2 cut(s)	374, 2559
XspI	CTAG	5 cut(s)	21, 791, 851, 1967, 2249
Zsp2I	ATGCAT	2 cut(s)	1148, 1202

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.