Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Reverse (-)

31993980 .. 31998293

Gene Structure 4314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1145700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2832 bp

ATGGGCTGGTTCAAAACCCCTAGCATTTTCACTTCAAACAATCACAGAAACCTTCCTTACTACAACCCGATTCGCCAAAACCTTGTAAACAACTCTGAAACTCCGAAAGATGGTGTCCCTAACAAGCTCTTTGGACTACTACGCAGGCTTCTGTCTGGAGGAAAAATGGATGGGGGGTCAAGGACTGAGGCAGAGGAGAAGCTCTACTCTTGGTTATACGCCTTGGCGCAGTCGGACAAGGATTTGGTTTTCGAGTATGTTCGTTCCACTGAAAGGGGATTGAGCTTTACTGAAGCTGAGAGGAGACTGAAGGAAAATGGCCCGAATGTTCCTGTGGATTATTCATTTCCGAGCTGGTGGCATATTTTGTGGAATGCTTTCTTTCATCCTTTCAATATCATATTGATCGTCTTGTCTGTAATCTCGTACATAACCAGTGACAGCCCAAATGGTTGTATCATGCTTGTTTTGGTTTTCATAAGCGTCTCCCTTCGATTCTATCAGGAATATGCCAGTTCAAAAGCGGCCATGAAACTCTCAGAATTCGTAAGGTGCCCAGTCAAAGTGCAAAGATGTGCAGGTAGAGTTGTTCAGACCGAACTAGTAGTACAAATTGATCAAAGAGATGTCGTTCCTGGCGACATTGTCATTTTCGAACCTGGAGACCTCTTTCCTGGTGATGTGAGGCTATTGTCTACGAAACACCTTGTTGTAAGTCAGTCCTCGTTAACAGGAGAGTCCTGGACAACTGAGAAAACAGCTGATGTCAGAGAAGATTCGAGCACTCCATTGCTAGATTTACGGAATATTTGCTTCATGGGAACAAATGTAGTATCAGGTGGCGGAGTTGGTCTAGTGGTGTCCACTGGATCTAAGACTTACATGAGCACCATGTTTTCAAACATAGGAAAGAAGAAACCACCAAATGAATTTGAGGATGGTGTTCGCCGCATATCTTACGTGCTGATTGCGGTTATGCTTGTAGTAGTCACCATCATAGTTGCAACTGACTACAGTACATCTCATGATCTATCTGAGAGCATCCTTTTCGGAATCTCGGTGGCAAGTGCACTCACTCCTCAAATGCTTCCCCTCATTGTTAACACAAGTCTTGCAAAAGGAGCACTTGCTATGGCCAAAGACAGATGCATTATCAAAAGTTTAACTGCAATACGAGACATGGGATCTATGGATATCTTATGCATTGACAAGACGGGTACGCTCACCATGAACCGTGCAATAATGGTTAATCATCTGGACAGCTGGGGATTACAGAAAGAAAAGGTTTTACAGTTTGCTTTCCTCAACTCCTATTTCAAGACCGACCAGAAGTATCCCCTTGATGATGCAATTTTGGCACATGTATATACCAATGGATACAAGTTCCAGCCTTCGAAATGGAAGAAAATAGATGAGATTCCTTTCGATTTTATACGAAGAAGAGTAGCTATTATCATGGAGAGAGAAGCAGAAGACAGAAGCCACCACATTTCCGATAGAATCGTGGTGACAAAAGGAGCTCTGGAAGAAGTGATGAAAGTTTGTTCTTTTATAGAGGATGTTGACGGGGAAACGAATATAACTTTCTCTACAGGACACTATCAAAGGATTTTAAATCTGACTGAGGAAATTAGCAATGATGGACTCAGAGCTATTGGAGTGGCAATAAGGAGGCTGGAAACGGAAACGAGCTATCAACACAGAGATGATGATGAAACCTTTGAATCAGACATGGTTTTCCTCGGCCTCATAACATTCTTTGACCCGCCCAAGGACTCAGCAAAGCAAGCTCTGTGGCGGTTGGCTGAGAAGGGAGTGAAAGCAAAGGTCTTGACAGGTGACTCGCTCACGCTATCAATAAGAGTTTGCAAGGAAGTTGGTATAAGAACCACCCATGTTGTTACAGGGCCAGAGCTTGAGTTACTTGACCAGGATTCCTTTCATGAGACGGTTAAAAGAGCAACTGTACTAGCTCGACTCACCCCAACACAGAAACTCCGAGTTGTTCAGTCCCTGCAGGCAGTTGGAAACCACACTGTTGGCTTCTTAGGAGATGGAGTAAATGACTCGCTTGCGCTGGATGCAGCCAATGTTGGTATATCAGTTGACTCAGGAGTATCAGTTGCGAAAGATTTTGCTGACATTATCTTACTCGAGAAGGATCTCAATGTCCTCATCGCTGGAGTTGAGCACGGTCGACTCACTTTTGGGAACACAATGAAGTACATAAAAATGTCGGTAATTGCCAATCTAGGAAGCGTTCTCTCAATTCTCATAGCAACCTTGGGTCTCAAGTATGAACCGTTGACTCCAAAACAGCTTCTTACGCAGAACTTCTTGTATAGTGTTGGCCAGATCGCAATCCCATGGGACAAAATGGAAGAAGAATATGTAAAAGTACCACAAAAATGGTCGAAGAAAGGCTTACCGATGTTTATTCTGTGGAATGGTCCTGTGTGCACTCTCTTTGACGTAAGCACACTTATGTTCCTTTGGTTCTATTATAAGGCTGACAGTCTGGATGACATTGTGTTCTTCCGTTCTGCTTGGTTCATCGAAGGGCTTCTGATGCAGACTCTGATCATCCACTTGATCCGGACAGAGAAAATTCCCTTCGTTCAGGAGTTTGCCTCATGGCCCGTGCTTTGTTCTACAGTTCTGATTTCTGCCATCGGAATTGCAATTCCATTCACACCAATTGGGGATGTGATGGGATTCGTTGAGCTTCCACTGTCGTACTTTGGATTTTTGGTGGTGCTTTTTGTAGGATATATTTTCGTCGGCCAGGTTGTCAAGAGACTATATATCATGGTTTACAAAAGTTGGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

944

Amino Acids

105.79

Weight (kDa)

6.21

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	80 - 142	9.7e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	195 - 299	2.5e-17	P-type ATPase actuator domain
Hydrolase	PF00702	398 - 696	1.8e-08	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	450 - 592	1.8e-20	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	768 - 934	1.8e-17	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2505
Acc36I	ACCTGC	1 cut(s)	569
AccB1I	GGYRCC	1 cut(s)	552
AccI	GTMKAC	2 cut(s)	695, 2197
AccIII	TCCGGA	1 cut(s)	2595
AciI	CCGC	6 cut(s)	524, 843, 949, 971, 1766, 1798
AclWI	GGATC	4 cut(s)	877, 1192, 2169, 2587
AcoI	YGGCCR	4 cut(s)	525, 1134, 2350, 2782
AcsI	RAATTY	3 cut(s)	542, 929, 2607
AcuI	CTGAAG	2 cut(s)	312, 329
AfaI	GTAC	8 cut(s)	428, 609, 1018, 1219, 1968, 2225, 2400, 2738
AfiI	CCNNNNNNNGG	3 cut(s)	110, 273, 1771
AflIII	ACRYGT	1 cut(s)	1360
AgsI	TTSAA	7 cut(s)	13, 36, 394, 519, 900, 1318, 1724
AhlI	ACTAGT	1 cut(s)	601
AjnI	CCWGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
Alw21I	GWGCWC	7 cut(s)	785, 890, 1072, 1126, 1522, 2193, 2462
Alw26I	GTCTC	7 cut(s)	298, 490, 657, 1170, 1940, 2294, 2791
Alw44I	GTGCAC	2 cut(s)	1068, 2458
AlwI	GGATC	4 cut(s)	877, 1192, 2169, 2587
AlwNI	CAGNNNCTG	2 cut(s)	2014, 2578
Ama87I	CYCGRG	1 cut(s)	2153
Aor13HI	TCCGGA	1 cut(s)	2595
AoxI	GGCC	8 cut(s)	319, 525, 1134, 1744, 1907, 2350, 2636, 2782
ApaLI	GTGCAC	2 cut(s)	1068, 2458
ApeKI	GCWGC	1 cut(s)	2084
ApoI	RAATTY	3 cut(s)	542, 929, 2607
Asp700I	GAANNNNTTC	2 cut(s)	377, 2562
AspLEI	GCGC	2 cut(s)	229, 2077
AspS9I	GGNCC	4 cut(s)	320, 1907, 2450, 2637
AsuHPI	GGTGA	6 cut(s)	689, 982, 1216, 1519, 1850, 1972
AsuII	TTCGAA	2 cut(s)	654, 1394
AvaI	CYCGRG	1 cut(s)	2153
AvaII	GGWCC	1 cut(s)	2450
BaeGI	GKGCMC	3 cut(s)	557, 1072, 2462
BalI	TGGCCA	2 cut(s)	1136, 2352
BanI	GGYRCC	1 cut(s)	552
BanII	GRGCYC	1 cut(s)	1522
BbsI	GAAGAC	1 cut(s)	1479
Bbv12I	GWGCWC	7 cut(s)	785, 890, 1072, 1126, 1522, 2193, 2462
BbvI	GCAGC	1 cut(s)	2096
BccI	CCATC	8 cut(s)	104, 164, 932, 1001, 1634, 2048, 2678, 2704
BcgI	CGANNNNNNTGC	2 cut(s)	1030, 1064
BciT130I	CCWGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
BciVI	GTATCC	2 cut(s)	1344, 1370
BclI	TGATCA	2 cut(s)	616, 2580
BcoDI	GTCTC	7 cut(s)	298, 490, 657, 1170, 1940, 2294, 2791
BcuI	ACTAGT	1 cut(s)	601
BfaI	CTAG	6 cut(s)	21, 602, 794, 854, 1970, 2252
BfmI	CTRYAG	4 cut(s)	1012, 1590, 2015, 2652
BfuAI	ACCTGC	1 cut(s)	569
BfuI	GTATCC	2 cut(s)	1344, 1370
BisI	GCNGC	3 cut(s)	525, 949, 2085
BlsI	GCNGC	3 cut(s)	526, 950, 2086
Bme1390I	CCNGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
Bme18I	GGWCC	1 cut(s)	2450
BmeT110I	CYCGRG	1 cut(s)	2153
BmgT120I	GGNCC	4 cut(s)	320, 1907, 2450, 2637
BmiI	GGNNCC	1 cut(s)	554
BmrFI	CCNGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
BmrI	ACTGGG	1 cut(s)	551
BmsI	GCATC	5 cut(s)	1050, 1136, 1336, 2071, 2559
BmuI	ACTGGG	1 cut(s)	551
BpiI	GAAGAC	1 cut(s)	1479
BplI	GAGNNNNNCTC	2 cut(s)	2615, 2647
BpmI	CTGGAG	3 cut(s)	177, 681, 2202
Bpu14I	TTCGAA	2 cut(s)	654, 1394
BpuEI	CTTGAG	2 cut(s)	1937, 2276
BsaAI	YACGTR	1 cut(s)	961
BsaBI	GATNNNNATC	1 cut(s)	2360
BsaI	GGTCTC	2 cut(s)	657, 2294
BsaJI	CCNNGG	5 cut(s)	222, 1741, 1770, 2283, 2366
BsaWI	WCCGGW	1 cut(s)	2595
BsaXI	ACNNNNNCTCC	6 cut(s)	1980, 2010, 2043, 2073, 2106, 2136
Bsc4I	CCNNNNNNNGG	3 cut(s)	110, 273, 1771
Bse1I	ACTGG	4 cut(s)	435, 513, 557, 871
Bse3DI	GCAATG	2 cut(s)	788, 1642
Bse8I	GATNNNNATC	1 cut(s)	2360
BseAI	TCCGGA	1 cut(s)	2595
BseBI	CCWGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
BseDI	CCNNGG	5 cut(s)	222, 1741, 1770, 2283, 2366
BseGI	GGATG	9 cut(s)	175, 385, 943, 1041, 1564, 2086, 2527, 2583, 2710
BseJI	GATNNNNATC	1 cut(s)	2360
BseLI	CCNNNNNNNGG	3 cut(s)	110, 273, 1771
BseMI	GCAATG	2 cut(s)	788, 1642
BseNI	ACTGG	4 cut(s)	435, 513, 557, 871
BseRI	GAGGAG	3 cut(s)	209, 316, 1068
BseSI	GKGCMC	3 cut(s)	557, 1072, 2462
BseXI	GCAGC	1 cut(s)	2096
BseYI	CCCAGC	1 cut(s)	1263
BsgI	GTGCAG	1 cut(s)	597
Bsh1285I	CGRYCG	1 cut(s)	2197
BshFI	GGCC	8 cut(s)	321, 527, 1136, 1746, 1909, 2352, 2638, 2784
BshNI	GGYRCC	1 cut(s)	552
BsiEI	CGRYCG	1 cut(s)	2197
BsiHKAI	GWGCWC	7 cut(s)	785, 890, 1072, 1126, 1522, 2193, 2462
BsiHKCI	CYCGRG	1 cut(s)	2153
BsiSI	CCGG	1 cut(s)	2596
BslFI	GGGAC	3 cut(s)	101, 1996, 2384
BslI	CCNNNNNNNGG	3 cut(s)	110, 273, 1771
BsmAI	GTCTC	7 cut(s)	298, 490, 657, 1170, 1940, 2294, 2791
BsmBI	CGTCTC	2 cut(s)	490, 1940
BsmFI	GGGAC	3 cut(s)	101, 1996, 2384
BsmI	GAATGC	1 cut(s)	379
BsnI	GGCC	8 cut(s)	321, 527, 1136, 1746, 1909, 2352, 2638, 2784
Bso31I	GGTCTC	2 cut(s)	657, 2294
BsoBI	CYCGRG	1 cut(s)	2153
Bsp119I	TTCGAA	2 cut(s)	654, 1394
Bsp1286I	GDGCHC	8 cut(s)	557, 785, 890, 1072, 1126, 1522, 2193, 2462
Bsp13I	TCCGGA	1 cut(s)	2595
Bsp143I	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
Bsp19I	CCATGG	1 cut(s)	2366
BspACI	CCGC	6 cut(s)	524, 843, 949, 971, 1766, 1798
BspANI	GGCC	8 cut(s)	321, 527, 1136, 1746, 1909, 2352, 2638, 2784
BspEI	TCCGGA	1 cut(s)	2595
BspHI	TCATGA	2 cut(s)	1024, 1942
BspLI	GGNNCC	1 cut(s)	554
BspMAI	CTGCAG	1 cut(s)	2019
BspMI	ACCTGC	1 cut(s)	569
BspPI	GGATC	4 cut(s)	877, 1192, 2169, 2587
BspT104I	TTCGAA	2 cut(s)	654, 1394
BspT107I	GGYRCC	1 cut(s)	552
BspTNI	GGTCTC	2 cut(s)	657, 2294
BsrDI	GCAATG	2 cut(s)	788, 1642
BsrI	ACTGG	4 cut(s)	435, 513, 557, 871
BssECI	CCNNGG	5 cut(s)	222, 1741, 1770, 2283, 2366
BssMI	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
BssT1I	CCWWGG	4 cut(s)	222, 1770, 2283, 2366
Bst2UI	CCWGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
Bst6I	CTCTTC	1 cut(s)	1435
BstBAI	YACGTR	1 cut(s)	961
BstBI	TTCGAA	2 cut(s)	654, 1394
BstC8I	GCNNGC	4 cut(s)	146, 1788, 2019, 2073
BstDSI	CCRYGG	1 cut(s)	2366
BstF5I	GGATG	9 cut(s)	175, 385, 943, 1041, 1564, 2086, 2527, 2583, 2710
BstHHI	GCGC	2 cut(s)	229, 2077
BstKTI	GATC	9 cut(s)	408, 619, 872, 1030, 1187, 2164, 2358, 2583, 2595
BstMAI	GTCTC	7 cut(s)	298, 490, 657, 1170, 1940, 2294, 2791
BstMBI	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
BstMCI	CGRYCG	1 cut(s)	2197
BstMWI	GCNNNNNNNGC	6 cut(s)	1121, 1355, 1787, 2081, 2326, 2569
BstNI	CCWGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
BstNSI	RCATGY	1 cut(s)	1364
BstSCI	CCNGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
BstSFI	CTRYAG	4 cut(s)	1012, 1590, 2015, 2652
BstSLI	GKGCMC	3 cut(s)	557, 1072, 2462
BstV1I	GCAGC	1 cut(s)	2096
BstV2I	GAAGAC	1 cut(s)	1479
BstX2I	RGATCY	3 cut(s)	869, 1184, 2161
BstXI	CCANNNNNNTGG	2 cut(s)	2039, 2409
BstYI	RGATCY	3 cut(s)	869, 1184, 2161
BsuI	GTATCC	2 cut(s)	1344, 1370
BsuRI	GGCC	8 cut(s)	321, 527, 1136, 1746, 1909, 2352, 2638, 2784
BtgI	CCRYGG	1 cut(s)	2366
BtgZI	GCGATG	1 cut(s)	2161
BtsCI	GGATG	9 cut(s)	175, 385, 943, 1041, 1564, 2086, 2527, 2583, 2710
BtsIMutI	CAGTG	5 cut(s)	267, 442, 864, 2034, 2729
BveI	ACCTGC	1 cut(s)	569
Cac8I	GCNNGC	4 cut(s)	146, 1788, 2019, 2073
CaiI	CAGNNNCTG	2 cut(s)	2014, 2578
CciI	TCATGA	2 cut(s)	1024, 1942
CfoI	GCGC	2 cut(s)	229, 2077
Cfr13I	GGNCC	4 cut(s)	320, 1907, 2450, 2637
CseI	GACGC	1 cut(s)	472
Csp6I	GTAC	8 cut(s)	427, 608, 1017, 1218, 1967, 2224, 2399, 2737
CspCI	CAANNNNNGTGG	4 cut(s)	1775, 1810, 1879, 1914
CviQI	GTAC	8 cut(s)	427, 608, 1017, 1218, 1967, 2224, 2399, 2737
DpnI	GATC	9 cut(s)	407, 618, 871, 1029, 1186, 2163, 2357, 2582, 2594
DpnII	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
DraI	TTTAAA	1 cut(s)	1614
EaeI	YGGCCR	4 cut(s)	525, 1134, 2350, 2782
Eam1104I	CTCTTC	1 cut(s)	1435
EarI	CTCTTC	1 cut(s)	1435
EciI	GGCGGA	1 cut(s)	858
Ecl136II	GAGCTC	1 cut(s)	1520
Eco130I	CCWWGG	4 cut(s)	222, 1770, 2283, 2366
Eco24I	GRGCYC	1 cut(s)	1522
Eco31I	GGTCTC	2 cut(s)	657, 2294
Eco32I	GATATC	1 cut(s)	1195
Eco47I	GGWCC	1 cut(s)	2450
Eco53kI	GAGCTC	1 cut(s)	1520
Eco57I	CTGAAG	2 cut(s)	312, 329
Eco88I	CYCGRG	1 cut(s)	2153
EcoICRI	GAGCTC	1 cut(s)	1520
EcoRI	GAATTC	1 cut(s)	542
EcoRII	CCWGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
EcoRV	GATATC	1 cut(s)	1195
EcoT14I	CCWWGG	4 cut(s)	222, 1770, 2283, 2366
EcoT22I	ATGCAT	2 cut(s)	1151, 1205
EcoT38I	GRGCYC	1 cut(s)	1522
ErhI	CCWWGG	4 cut(s)	222, 1770, 2283, 2366
Esp3I	CGTCTC	2 cut(s)	490, 1940
FalI	AAGNNNNNCTT	4 cut(s)	1110, 1142, 2408, 2440
FaqI	GGGAC	3 cut(s)	101, 1996, 2384
FauI	CCCGC	1 cut(s)	1773
FbaI	TGATCA	2 cut(s)	616, 2580
FblI	GTMKAC	2 cut(s)	695, 2197
Fnu4HI	GCNGC	3 cut(s)	525, 949, 2085
FokI	GGATG	9 cut(s)	182, 372, 950, 1028, 1571, 2093, 2534, 2570, 2717
FriOI	GRGCYC	1 cut(s)	1522
Fsp4HI	GCNGC	3 cut(s)	525, 949, 2085
FspBI	CTAG	6 cut(s)	21, 602, 794, 854, 1970, 2252
GlaI	GCGC	2 cut(s)	228, 2076
GluI	GCNGC	3 cut(s)	525, 949, 2085
GsaI	CCCAGC	1 cut(s)	1267
GsuI	CTGGAG	3 cut(s)	177, 681, 2202
HaeIII	GGCC	8 cut(s)	321, 527, 1136, 1746, 1909, 2352, 2638, 2784
HapII	CCGG	1 cut(s)	2596
HgaI	GACGC	1 cut(s)	472
HhaI	GCGC	2 cut(s)	229, 2077
Hin6I	GCGC	2 cut(s)	227, 2075
HinP1I	GCGC	2 cut(s)	227, 2075
HincII	GTYRAC	6 cut(s)	729, 1102, 1564, 2107, 2198, 2307
HindII	GTYRAC	6 cut(s)	729, 1102, 1564, 2107, 2198, 2307
HpaI	GTTAAC	2 cut(s)	729, 1102
HpaII	CCGG	1 cut(s)	2596
HphI	GGTGA	6 cut(s)	689, 982, 1216, 1519, 1850, 1972
Hpy99I	CGWCG	1 cut(s)	2783
HpyAV	CCTTC	8 cut(s)	62, 304, 500, 1401, 1804, 2152, 2552, 2623
HpyCH4IV	ACGT	2 cut(s)	960, 2472
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1121, 1355, 1787, 2081, 2326, 2569
HpySE526I	ACGT	2 cut(s)	960, 2472
HspAI	GCGC	2 cut(s)	227, 2075
Kpn2I	TCCGGA	1 cut(s)	2595
Ksp22I	TGATCA	2 cut(s)	616, 2580
KspAI	GTTAAC	2 cut(s)	729, 1102
Kzo9I	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
LmnI	GCTCC	2 cut(s)	1121, 1517
Lsp1109I	GCAGC	1 cut(s)	2096
LweI	GCATC	5 cut(s)	1050, 1136, 1336, 2071, 2559
MaeI	CTAG	6 cut(s)	21, 602, 794, 854, 1970, 2252
MaeII	ACGT	2 cut(s)	960, 2472
MaeIII	GTNAC	6 cut(s)	437, 988, 1507, 1838, 1900, 1920
MalI	GATC	9 cut(s)	407, 618, 871, 1029, 1186, 2163, 2357, 2582, 2594
MboI	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
MfeI	CAATTG	1 cut(s)	2697
MflI	RGATCY	3 cut(s)	869, 1184, 2161
MhlI	GDGCHC	8 cut(s)	557, 785, 890, 1072, 1126, 1522, 2193, 2462
MlsI	TGGCCA	2 cut(s)	1136, 2352
MluNI	TGGCCA	2 cut(s)	1136, 2352
MmeI	TCCRAC	2 cut(s)	213, 2005
Mox20I	TGGCCA	2 cut(s)	1136, 2352
Mph1103I	ATGCAT	2 cut(s)	1151, 1205
MroI	TCCGGA	1 cut(s)	2595
MroXI	GAANNNNTTC	2 cut(s)	377, 2562
MscI	TGGCCA	2 cut(s)	1136, 2352
MseI	TTAA	7 cut(s)	728, 1101, 1163, 1248, 1613, 1953, 2830
MslI	CAYNNNNRTG	4 cut(s)	1704, 2231, 2407, 2483
Msp20I	TGGCCA	2 cut(s)	1136, 2352
MspA1I	CMGCKG	2 cut(s)	761, 1263
MspI	CCGG	1 cut(s)	2596
MspR9I	CCNGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
MunI	CAATTG	1 cut(s)	2697
Mva1269I	GAATGC	1 cut(s)	379
MvaI	CCWGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
MwoI	GCNNNNNNNGC	6 cut(s)	1121, 1355, 1787, 2081, 2326, 2569
NcoI	CCATGG	1 cut(s)	2366
NdeII	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
NlaIV	GGNNCC	1 cut(s)	554
NmeAIII	GCCGAG	1 cut(s)	1722
NmuCI	GTSAC	4 cut(s)	437, 988, 1507, 1838
NsiI	ATGCAT	2 cut(s)	1151, 1205
NspI	RCATGY	1 cut(s)	1364
NspV	TTCGAA	2 cut(s)	654, 1394
PaeR7I	CTCGAG	1 cut(s)	2153
PagI	TCATGA	2 cut(s)	1024, 1942
PciI	ACATGT	1 cut(s)	1360
PcsI	WCGNNNNNNNCGW	1 cut(s)	636
PctI	GAATGC	1 cut(s)	379
PdmI	GAANNNNTTC	2 cut(s)	377, 2562
PfeI	GAWTC	9 cut(s)	70, 495, 776, 1053, 1417, 1500, 1724, 1934, 2715
PflFI	GACNNNGTC	1 cut(s)	644
PfoI	TCCNGGA	1 cut(s)	740
PkrI	GCNGC	3 cut(s)	526, 950, 2086
Ppu21I	YACGTR	1 cut(s)	961
PscI	ACATGT	1 cut(s)	1360
PsiI	TTATAA	1 cut(s)	2505
Psp124BI	GAGCTC	1 cut(s)	1522
Psp6I	CCWGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
PspFI	CCCAGC	1 cut(s)	1263
PspGI	CCWGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
PspN4I	GGNNCC	1 cut(s)	554
PspPI	GGNCC	4 cut(s)	320, 1907, 2450, 2637
PsrI	GAACNNNNNNTAC	2 cut(s)	591, 623
PstI	CTGCAG	1 cut(s)	2019
PstNI	CAGNNNCTG	2 cut(s)	2014, 2578
PsuI	RGATCY	3 cut(s)	869, 1184, 2161
PsyI	GACNNNGTC	1 cut(s)	644
PvuII	CAGCTG	2 cut(s)	761, 1263
RsaI	GTAC	8 cut(s)	428, 609, 1018, 1219, 1968, 2225, 2400, 2738
RsaNI	GTAC	8 cut(s)	427, 608, 1017, 1218, 1967, 2224, 2399, 2737
RseI	CAYNNNNRTG	4 cut(s)	1704, 2231, 2407, 2483
SacI	GAGCTC	1 cut(s)	1522
SalI	GTCGAC	1 cut(s)	2196
SaqAI	TTAA	7 cut(s)	728, 1101, 1163, 1248, 1613, 1953, 2830
SatI	GCNGC	3 cut(s)	525, 949, 2085
Sau3AI	GATC	9 cut(s)	405, 616, 869, 1027, 1184, 2161, 2355, 2580, 2592
Sau96I	GGNCC	4 cut(s)	320, 1907, 2450, 2637
SbfI	CCTGCAGG	1 cut(s)	2019
ScrFI	CCNGG	6 cut(s)	636, 660, 675, 742, 1931, 2786
SdaI	CCTGCAGG	1 cut(s)	2019
SduI	GDGCHC	8 cut(s)	557, 785, 890, 1072, 1126, 1522, 2193, 2462
SfaNI	GCATC	5 cut(s)	1050, 1136, 1336, 2071, 2559
SfcI	CTRYAG	4 cut(s)	1012, 1590, 2015, 2652
Sfr274I	CTCGAG	1 cut(s)	2153
SfuI	TTCGAA	2 cut(s)	654, 1394
SinI	GGWCC	1 cut(s)	2450
SlaI	CTCGAG	1 cut(s)	2153
SmiMI	CAYNNNNRTG	4 cut(s)	1704, 2231, 2407, 2483
SmlI	CTYRAG	3 cut(s)	1916, 2153, 2291
SmoI	CTYRAG	3 cut(s)	1916, 2153, 2291
SpeI	ACTAGT	1 cut(s)	601
Sse8387I	CCTGCAGG	1 cut(s)	2019
SsiI	CCGC	6 cut(s)	524, 843, 949, 971, 1766, 1798
SspI	AATATT	1 cut(s)	808
SspMI	CTAG	6 cut(s)	21, 602, 794, 854, 1970, 2252
SstI	GAGCTC	1 cut(s)	1522
StyD4I	CCNGG	6 cut(s)	634, 658, 673, 740, 1929, 2784
StyI	CCWWGG	4 cut(s)	222, 1770, 2283, 2366
TaiI	ACGT	2 cut(s)	963, 2475
TaqII	GACCGA	2 cut(s)	611, 1337
TatI	WGTACW	4 cut(s)	607, 1016, 1966, 2223
TauI	GCSGC	2 cut(s)	527, 951
TfiI	GAWTC	9 cut(s)	70, 495, 776, 1053, 1417, 1500, 1724, 1934, 2715
Tru1I	TTAA	7 cut(s)	728, 1101, 1163, 1248, 1613, 1953, 2830
Tru9I	TTAA	7 cut(s)	728, 1101, 1163, 1248, 1613, 1953, 2830
TscAI	CASTG	5 cut(s)	274, 442, 871, 2041, 2736
TseFI	GTSAC	4 cut(s)	437, 988, 1507, 1838
TseI	GCWGC	1 cut(s)	2084
Tsp45I	GTSAC	4 cut(s)	437, 988, 1507, 1838
TspGWI	ACGGA	3 cut(s)	817, 1697, 2528
TspRI	CASTG	5 cut(s)	274, 442, 871, 2041, 2736
Tth111I	GACNNNGTC	1 cut(s)	644
VneI	GTGCAC	2 cut(s)	1068, 2458
VpaK11BI	GGWCC	1 cut(s)	2450
XapI	RAATTY	3 cut(s)	542, 929, 2607
XceI	RCATGY	1 cut(s)	1364
XhoI	CTCGAG	1 cut(s)	2153
XmiI	GTMKAC	2 cut(s)	695, 2197
XmnI	GAANNNNTTC	2 cut(s)	377, 2562
XspI	CTAG	6 cut(s)	21, 602, 794, 854, 1970, 2252
Zsp2I	ATGCAT	2 cut(s)	1151, 1205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.