Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Forward (+)

23643982 .. 23648385

Gene Structure 4404 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017957

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGGGAACTTTCAAAATCCCCACCATTTTTACTTCTAAAAGCCACCCGAGGCTTCCTTACCAAAACCCCATTCGCCAAAATCTAGTAGACAAACCTGAAAGCCAGAATGGCTCCAACAGGGTGGTTAGATTCTTGCGCGGGCTTATGTCCGGAGGGAAAATTGATGGGGGGTCGAGGACAGAGGCAGAGGAGAAGCTTTACTCTTGGTTATACGCCTTGGCGCAATCCGATAAGGATTTGGTTTTCGAGTATGTTCGATCCACCGAAAGAGGATTGAGCTTTACTGAAGCTGAAAGGAGATTGAAGGAAAATGGCCCGAATGTTCCTGTTGATTTCTCTTTTCCTAGCTGGTGGCATTTTTTATGGAGTGCTTTCTTTCATCCTTTTAATATCATATTGATCGTCCTGTCTGTAATCTCGTACATAACCAGCGACAGCCCAAATGGATGCATCATGCTTGTTTTGGTTTTGATAAGTGTTTGCCTCCGATTCTATCAGGAATACGGAAGTTCAAAAGCAGCCATGGAACTTTCAGAATTTGTAAGGTGCCCAGTCAAAGTTCAAAGATGTGCAGGTAGAGTTGTTCAGACTGAATTAGTGGTACAAATTGATCAAAGAGATATTGTTCCTGGTGATATTATCATATTTGAACCTGGAGACCTTTTTCCTGGAGATGTGAGACTATTGTCTTCGAAACACCTTGTTGTAAGTCAGGCCTCATTAACTGGAGAGTCCTGGACAACCGAAAAAACAGCTGATATCAGAGAGAATCAAAGCACTCCATTGCTAGATTTAAGGAATATTTGCTTCATGGGAACAAATGTAGTATCAGGCAGTGGATCTGGTCTAGTGGTTTCCACTGGATCTAAGACATACATGAGCACCATGTTTTCAAACATAGGGAAGAAGAAACCACCAAATGAATTTGAGGATGGTGTTCGTCGCATATCTTATGTGCTGGTTGCTGTTATGCTAGTAGTAGTCACCATCATAGTTATAACTGACTACTCTACATCTCGTGATCTGTCTGAGAGCATCCTTTTTGGAGTGTCAGTGGCAAGTGCACTTACTCCTCAAATGCTTCCCCTGATCGTTAACACAAGTCTTGCAAAAGGAGCACTTGCTATGGCCAGAGACAGATGCATAATCAAAAGCTTATCTGCAATACGAAACATGGGTTCTATGGATATCTTATGCATTGACAAGACTGGTACACTCACCATGAATCGTGCGATAATGGTTAATTATCTGGACAGCTGGGGGTTAGACAAAGAAAAGGTTTTACAGTTTGCTTTCCTCAACTCATATTTCAAGACCGATCAGAAATATCCTTTGGATGATGCAATTTTGGCACATGTATATACCAATGGATTCAGGTTCCAACCGTCAAAATGGAAGAAACTAGATGAGATTCCTTTTGATTTCATAAGAAGAAGGGTATCTATTATCATGGAAAGAGAAGAAGACACTGACCCTCACAGTGTTGTGAGAGTCATGGTGACAAAAGGAGCTCTGGAAGAAGTAATGAAAGTTTGTTCTTTTATGGAGAATGTTGACAGTGGCACGATTTCACCTCTCTCTCCAGAACAGTATCAAAGGATTATAAATATGACCGAGGAAATAAGCAATGAGGGACTGAGAGTTATAGGAGTAGCAACAAAGAAGCTGGAAAAGCAGATAAGGTATGAGCGCAAAGATAATGATGATACTTCTGAATCAGACATGGTTTTCCTCGGCCTCATAACATTCTTTGACCCACCCAAGGACTCAGCAAAGCAAGCTCTGTGGCGGTTGGCTGAGAAGGGAGTGAAAGCAAAAGTATTAACAGGTGACTCACTGTCTCTATCTATAAGAGTTTGCAAGGAAGTTGGTATCAGAACAACTCATGTAGTTACGGGGCCAGAGCTTGAGCTACTCGACCAGGATGCCTTTCATGAGACTGTTAAAACAGCAACGGTCTTAGCTCGACTCACCCCAACGCAGAAACTCCGAGTTGTGCAGTCCTTGCAAACAATTGGTAACCACATTGTTGGATTTTTGGGAGATGGAGTAAATGACTCGCTTGCACTGGATGCAGCCCATGTTGGTATATCAGTTGATTCAGGAGCATCAGTTGCAAAAGACTTTGCTGACATTATCTTACTGGAGAAAGACCTGAATGTACTCATTGCCGGAGTTGAACATGGCCGACTCACTTTCGGGAACACAATGAAGTACATAAAAATGTCAGTTATTGCCAATCTGGGAAGCGTTCTCTCAATTCTCATAGCAACTCTGGTGCTCAAGTATGAGCCATTGACGGCAAGGCAGCTTCTTACACAGAACTTCTTGTATAGTGTGGGCCAGATTGCAATCCCATGGGATAAAATGGAAGATGATTATGTAAAAGTCCCACAAAGATGGTCAAAGAAAGGTTTGCCGATGTTCATTTTGTGGAATGGACCTGTCTGCACTCTTTTTGATGTTACTACACTTCTGTTCCTTTGGTTCTATTATAAAGCTGATAGTTTGGAGGATCTTGCTTTCTTCCACACTGCTTGGTTCATCGAAGGGCTTCTCATGCAGACCCTAATCATCCACTTGATCCGTACAGAGAAAATTCCTTTCATTCAGGAGTTTGCCTCATGGCCTGTGCTTTGTTCTACAGTTCTGGTTTCTGCAATTGGAATCGCAATTACATTCACCCCGATTGGGAAAGTGATGGGATTTATCAGGCTTCCACTGTCATACTTTGGGTTTTTGGTAGTACTGTTTATAGGATATTTTGTTGTTGGCCAGGTGGTCAAGAGACTCTACATTTTGGCTTATAAAACCTGGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

941

Amino Acids

105.43

Weight (kDa)

7.18

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	78 - 140	8.4e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	193 - 297	3.2e-17	P-type ATPase actuator domain
Hydrolase	PF00702	397 - 693	4.9e-08	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	462 - 589	2.5e-23	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	765 - 931	1.2e-18	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	998, 1604, 2496, 2808
Acc36I	ACCTGC	1 cut(s)	563
AccB1I	GGYRCC	1 cut(s)	546
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	1 cut(s)	138
AccIII	TCCGGA	1 cut(s)	149
AciI	CCGC	2 cut(s)	138, 1789
AclWI	GGATC	5 cut(s)	252, 847, 871, 2523, 2578
AcoI	YGGCCR	3 cut(s)	1128, 2185, 2773
AcsI	RAATTY	3 cut(s)	536, 923, 2598
AcuI	CTGAAG	1 cut(s)	306
AfaI	GTAC	7 cut(s)	422, 603, 1213, 2163, 2216, 2590, 2748
AfiI	CCNNNNNNNGG	2 cut(s)	1762, 2691
AflIII	ACRYGT	1 cut(s)	1354
AgsI	TTSAA	8 cut(s)	13, 304, 513, 563, 650, 894, 1312, 2180
AhdI	GACNNNNNGTC	1 cut(s)	1837
AjnI	CCWGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
AjuI	GAANNNNNNNTTGG	4 cut(s)	54, 86, 1316, 1348
Alw21I	GWGCWC	5 cut(s)	884, 1066, 1120, 1513, 2283
Alw26I	GTCTC	6 cut(s)	651, 673, 1128, 1845, 1931, 2782
Alw44I	GTGCAC	1 cut(s)	1062
AlwI	GGATC	5 cut(s)	252, 847, 871, 2523, 2578
Ama87I	CYCGRG	1 cut(s)	46
Aor13HI	TCCGGA	1 cut(s)	149
AoxI	GGCC	9 cut(s)	313, 714, 1128, 1735, 1898, 2185, 2341, 2627, 2773
ApaLI	GTGCAC	1 cut(s)	1062
ApeKI	GCWGC	3 cut(s)	518, 2075, 2308
ApoI	RAATTY	3 cut(s)	536, 923, 2598
Asp700I	GAANNNNTTC	1 cut(s)	2553
AspLEI	GCGC	3 cut(s)	138, 223, 1692
AspS9I	GGNCC	4 cut(s)	314, 1898, 2341, 2441
AsuHPI	GGTGA	8 cut(s)	644, 976, 1210, 1510, 1563, 1841, 1963, 2674
AsuII	TTCGAA	1 cut(s)	692
AvaI	CYCGRG	1 cut(s)	46
AvaII	GGWCC	1 cut(s)	2441
BaeGI	GKGCMC	2 cut(s)	551, 1066
BalI	TGGCCA	2 cut(s)	1130, 2775
BanI	GGYRCC	1 cut(s)	546
BanII	GRGCYC	1 cut(s)	1513
BauI	CACGAG	1 cut(s)	1017
BbsI	GAAGAC	2 cut(s)	681, 1470
Bbv12I	GWGCWC	5 cut(s)	884, 1066, 1120, 1513, 2283
BbvI	GCAGC	3 cut(s)	530, 2087, 2320
BccI	CCATC	6 cut(s)	158, 926, 995, 2039, 2394, 2695
BceAI	ACGGC	1 cut(s)	2316
BcgI	CGANNNNNNTGC	2 cut(s)	1907, 1941
BciT130I	CCWGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
BclI	TGATCA	1 cut(s)	610
BcoDI	GTCTC	6 cut(s)	651, 673, 1128, 1845, 1931, 2782
BfaI	CTAG	6 cut(s)	83, 345, 788, 848, 974, 1403
BfmI	CTRYAG	1 cut(s)	2643
BfuAI	ACCTGC	1 cut(s)	563
BglI	GCCNNNNNGGC	1 cut(s)	108
BisI	GCNGC	3 cut(s)	519, 2076, 2309
BlsI	GCNGC	3 cut(s)	520, 2077, 2310
BmcAI	AGTACT	1 cut(s)	2748
Bme1390I	CCNGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
Bme18I	GGWCC	1 cut(s)	2441
BmeRI	GACNNNNNGTC	1 cut(s)	1837
BmeT110I	CYCGRG	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	314, 1898, 2341, 2441
BmiI	GGNNCC	4 cut(s)	112, 548, 1379, 1899
BmrFI	CCNGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
BmrI	ACTGGG	1 cut(s)	545
BmsI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1915, 2062, 2117
BmuI	ACTGGG	1 cut(s)	545
BoxI	GACNNNNGTC	1 cut(s)	685
BpiI	GAAGAC	2 cut(s)	681, 1470
BplI	GAGNNNNNCTC	2 cut(s)	2606, 2638
BpmI	CTGGAG	5 cut(s)	675, 690, 747, 1566, 2165
Bpu14I	TTCGAA	1 cut(s)	692
BpuEI	CTTGAG	2 cut(s)	1928, 2267
BsaBI	GATNNNNATC	1 cut(s)	2351
BsaI	GGTCTC	1 cut(s)	651
BsaJI	CCNNGG	7 cut(s)	47, 216, 522, 1614, 1732, 1761, 2357
BsaWI	WCCGGW	1 cut(s)	149
BsaXI	ACNNNNNCTCC	6 cut(s)	1038, 1068, 2034, 2064, 2097, 2127
Bsc4I	CCNNNNNNNGG	2 cut(s)	1762, 2691
Bse1I	ACTGG	6 cut(s)	551, 730, 865, 1213, 2073, 2148
Bse3DI	GCAATG	3 cut(s)	782, 1633, 2166
Bse8I	GATNNNNATC	1 cut(s)	2351
BseAI	TCCGGA	1 cut(s)	149
BseBI	CCWGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
BseDI	CCNNGG	7 cut(s)	47, 216, 522, 1614, 1732, 1761, 2357
BseGI	GGATG	8 cut(s)	379, 452, 937, 1035, 1342, 1930, 2077, 2574
BseJI	GATNNNNATC	1 cut(s)	2351
BseLI	CCNNNNNNNGG	2 cut(s)	1762, 2691
BseMI	GCAATG	3 cut(s)	782, 1633, 2166
BseMII	CTCAG	4 cut(s)	1020, 1628, 1782, 1788
BseNI	ACTGG	6 cut(s)	551, 730, 865, 1213, 2073, 2148
BseRI	GAGGAG	2 cut(s)	203, 1062
BseSI	GKGCMC	2 cut(s)	551, 1066
BseXI	GCAGC	3 cut(s)	530, 2087, 2320
BseYI	CCCAGC	1 cut(s)	1257
BsgI	GTGCAG	3 cut(s)	591, 2018, 2434
Bsh1236I	CGCG	1 cut(s)	138
BshFI	GGCC	9 cut(s)	315, 716, 1130, 1737, 1900, 2187, 2343, 2629, 2775
BshNI	GGYRCC	1 cut(s)	546
BsiHKAI	GWGCWC	5 cut(s)	884, 1066, 1120, 1513, 2283
BsiHKCI	CYCGRG	1 cut(s)	46
BsiSI	CCGG	2 cut(s)	150, 2172
BslFI	GGGAC	2 cut(s)	1647, 2375
BslI	CCNNNNNNNGG	2 cut(s)	1762, 2691
BsmAI	GTCTC	6 cut(s)	651, 673, 1128, 1845, 1931, 2782
BsmFI	GGGAC	2 cut(s)	1647, 2375
BsnI	GGCC	9 cut(s)	315, 716, 1130, 1737, 1900, 2187, 2343, 2629, 2775
Bso31I	GGTCTC	1 cut(s)	651
BsoBI	CYCGRG	1 cut(s)	46
Bsp119I	TTCGAA	1 cut(s)	692
Bsp1286I	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1513, 2283
Bsp13I	TCCGGA	1 cut(s)	149
Bsp19I	CCATGG	2 cut(s)	522, 2357
BspACI	CCGC	2 cut(s)	138, 1789
BspANI	GGCC	9 cut(s)	315, 716, 1130, 1737, 1900, 2187, 2343, 2629, 2775
BspCNI	CTCAG	4 cut(s)	1021, 1629, 1781, 1789
BspEI	TCCGGA	1 cut(s)	149
BspFNI	CGCG	1 cut(s)	138
BspHI	TCATGA	1 cut(s)	1933
BspLI	GGNNCC	4 cut(s)	112, 548, 1379, 1899
BspMI	ACCTGC	1 cut(s)	563
BspPI	GGATC	5 cut(s)	252, 847, 871, 2523, 2578
BspT104I	TTCGAA	1 cut(s)	692
BspT107I	GGYRCC	1 cut(s)	546
BspTNI	GGTCTC	1 cut(s)	651
BsrDI	GCAATG	3 cut(s)	782, 1633, 2166
BsrI	ACTGG	6 cut(s)	551, 730, 865, 1213, 2073, 2148
BssECI	CCNNGG	7 cut(s)	47, 216, 522, 1614, 1732, 1761, 2357
BssSI	CACGAG	1 cut(s)	1017
BssT1I	CCWWGG	4 cut(s)	216, 522, 1761, 2357
Bst2BI	CACGAG	1 cut(s)	1017
Bst2UI	CCWGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
BstAPI	GCANNNNNTGC	3 cut(s)	2005, 2072, 2114
BstBI	TTCGAA	1 cut(s)	692
BstC8I	GCNNGC	3 cut(s)	140, 1779, 2064
BstDEI	CTNAG	6 cut(s)	867, 1029, 1637, 1768, 1797, 1960
BstDSI	CCRYGG	2 cut(s)	522, 2357
BstEII	GGTNACC	1 cut(s)	2018
BstF5I	GGATG	8 cut(s)	379, 452, 937, 1035, 1342, 1930, 2077, 2574
BstFNI	CGCG	1 cut(s)	138
BstHHI	GCGC	3 cut(s)	138, 223, 1692
BstMAI	GTCTC	6 cut(s)	651, 673, 1128, 1845, 1931, 2782
BstMWI	GCNNNNNNNGC	9 cut(s)	108, 1115, 1349, 1672, 1778, 2005, 2072, 2114, 2560
BstNI	CCWGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
BstNSI	RCATGY	1 cut(s)	1358
BstPAI	GACNNNNGTC	1 cut(s)	685
BstPI	GGTNACC	1 cut(s)	2018
BstSCI	CCNGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
BstSFI	CTRYAG	1 cut(s)	2643
BstSLI	GKGCMC	2 cut(s)	551, 1066
BstUI	CGCG	1 cut(s)	138
BstV1I	GCAGC	3 cut(s)	530, 2087, 2320
BstV2I	GAAGAC	2 cut(s)	681, 1470
BstX2I	RGATCY	3 cut(s)	839, 863, 2515
BstXI	CCANNNNNNTGG	3 cut(s)	121, 2030, 2400
BstYI	RGATCY	3 cut(s)	839, 863, 2515
BsuRI	GGCC	9 cut(s)	315, 716, 1130, 1737, 1900, 2187, 2343, 2629, 2775
BtgI	CCRYGG	2 cut(s)	522, 2357
BtsCI	GGATG	8 cut(s)	379, 452, 937, 1035, 1342, 1930, 2077, 2574
BtsI	GCAGTG	2 cut(s)	841, 2532
BveI	ACCTGC	1 cut(s)	563
Cac8I	GCNNGC	3 cut(s)	140, 1779, 2064
CciI	TCATGA	1 cut(s)	1933
CfoI	GCGC	3 cut(s)	138, 223, 1692
Cfr13I	GGNCC	4 cut(s)	314, 1898, 2341, 2441
Csp6I	GTAC	7 cut(s)	421, 602, 1212, 2162, 2215, 2589, 2747
CspCI	CAANNNNNGTGG	4 cut(s)	1766, 1801, 2519, 2554
CviQI	GTAC	7 cut(s)	421, 602, 1212, 2162, 2215, 2589, 2747
DdeI	CTNAG	6 cut(s)	867, 1029, 1637, 1768, 1797, 1960
DriI	GACNNNNNGTC	1 cut(s)	1837
EaeI	YGGCCR	3 cut(s)	1128, 2185, 2773
Eam1105I	GACNNNNNGTC	1 cut(s)	1837
Ecl136II	GAGCTC	1 cut(s)	1511
Eco130I	CCWWGG	4 cut(s)	216, 522, 1761, 2357
Eco147I	AGGCCT	1 cut(s)	716
Eco24I	GRGCYC	1 cut(s)	1513
Eco31I	GGTCTC	1 cut(s)	651
Eco32I	GATATC	2 cut(s)	760, 1189
Eco47I	GGWCC	1 cut(s)	2441
Eco53kI	GAGCTC	1 cut(s)	1511
Eco57I	CTGAAG	1 cut(s)	306
Eco88I	CYCGRG	1 cut(s)	46
Eco91I	GGTNACC	1 cut(s)	2018
EcoICRI	GAGCTC	1 cut(s)	1511
EcoO65I	GGTNACC	1 cut(s)	2018
EcoRII	CCWGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
EcoRV	GATATC	2 cut(s)	760, 1189
EcoT14I	CCWWGG	4 cut(s)	216, 522, 1761, 2357
EcoT22I	ATGCAT	3 cut(s)	452, 1145, 1199
EcoT38I	GRGCYC	1 cut(s)	1513
ErhI	CCWWGG	4 cut(s)	216, 522, 1761, 2357
FalI	AAGNNNNNCTT	2 cut(s)	1104, 1136
FaqI	GGGAC	2 cut(s)	1647, 2375
FauI	CCCGC	1 cut(s)	131
FbaI	TGATCA	1 cut(s)	610
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	3 cut(s)	519, 2076, 2309
FokI	GGATG	8 cut(s)	366, 459, 944, 1022, 1349, 1937, 2084, 2561
FriOI	GRGCYC	1 cut(s)	1513
Fsp4HI	GCNGC	3 cut(s)	519, 2076, 2309
FspBI	CTAG	6 cut(s)	83, 345, 788, 848, 974, 1403
GlaI	GCGC	3 cut(s)	137, 222, 1691
GluI	GCNGC	3 cut(s)	519, 2076, 2309
GsaI	CCCAGC	1 cut(s)	1261
GsuI	CTGGAG	5 cut(s)	675, 690, 747, 1566, 2165
HaeIII	GGCC	9 cut(s)	315, 716, 1130, 1737, 1900, 2187, 2343, 2629, 2775
HapII	CCGG	2 cut(s)	150, 2172
HhaI	GCGC	3 cut(s)	138, 223, 1692
Hin6I	GCGC	3 cut(s)	136, 221, 1690
HinP1I	GCGC	3 cut(s)	136, 221, 1690
HincII	GTYRAC	2 cut(s)	1096, 1555
HindII	GTYRAC	2 cut(s)	1096, 1555
HindIII	AAGCTT	2 cut(s)	194, 1153
HpaI	GTTAAC	1 cut(s)	1096
HpaII	CCGG	2 cut(s)	150, 2172
HphI	GGTGA	8 cut(s)	644, 976, 1210, 1510, 1563, 1841, 1963, 2674
Hpy166II	GTNNAC	5 cut(s)	88, 1064, 1096, 1214, 1555
Hpy8I	GTNNAC	5 cut(s)	88, 1064, 1096, 1214, 1555
Hpy99I	CGWCG	1 cut(s)	945
HpyAV	CCTTC	4 cut(s)	298, 1428, 1795, 2543
HpyF10VI	GCNNNNNNNGC	9 cut(s)	108, 1115, 1349, 1672, 1778, 2005, 2072, 2114, 2560
HpyF3I	CTNAG	6 cut(s)	867, 1029, 1637, 1768, 1797, 1960
HspAI	GCGC	3 cut(s)	136, 221, 1690
Kpn2I	TCCGGA	1 cut(s)	149
Ksp22I	TGATCA	1 cut(s)	610
KspAI	GTTAAC	1 cut(s)	1096
LmnI	GCTCC	4 cut(s)	116, 1115, 1508, 2105
Lsp1109I	GCAGC	3 cut(s)	530, 2087, 2320
LweI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1915, 2062, 2117
MaeI	CTAG	6 cut(s)	83, 345, 788, 848, 974, 1403
MaeIII	GTNAC	6 cut(s)	982, 1498, 1829, 1891, 2018, 2464
MfeI	CAATTG	2 cut(s)	2013, 2661
MflI	RGATCY	3 cut(s)	839, 863, 2515
MhlI	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1513, 2283
MlsI	TGGCCA	2 cut(s)	1130, 2775
MluNI	TGGCCA	2 cut(s)	1130, 2775
MlyI	GAGTC	8 cut(s)	740, 1500, 1760, 1826, 1962, 2051, 2184, 2784
MmeI	TCCRAC	3 cut(s)	138, 1405, 2011
Mox20I	TGGCCA	2 cut(s)	1130, 2775
Mph1103I	ATGCAT	3 cut(s)	452, 1145, 1199
MroI	TCCGGA	1 cut(s)	149
MroXI	GAANNNNTTC	1 cut(s)	2553
MscI	TGGCCA	2 cut(s)	1130, 2775
MseI	TTAA	7 cut(s)	387, 722, 794, 1095, 1242, 1823, 1944
MslI	CAYNNNNRTG	2 cut(s)	2222, 2398
Msp20I	TGGCCA	2 cut(s)	1130, 2775
MspA1I	CMGCKG	2 cut(s)	755, 1257
MspI	CCGG	2 cut(s)	150, 2172
MspR9I	CCNGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
MunI	CAATTG	2 cut(s)	2013, 2661
MvaI	CCWGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
MvnI	CGCG	1 cut(s)	138
MwoI	GCNNNNNNNGC	9 cut(s)	108, 1115, 1349, 1672, 1778, 2005, 2072, 2114, 2560
NcoI	CCATGG	2 cut(s)	522, 2357
NlaIV	GGNNCC	4 cut(s)	112, 548, 1379, 1899
NmeAIII	GCCGAG	1 cut(s)	1713
NmuCI	GTSAC	3 cut(s)	982, 1498, 1829
NsiI	ATGCAT	3 cut(s)	452, 1145, 1199
NspI	RCATGY	1 cut(s)	1358
NspV	TTCGAA	1 cut(s)	692
PagI	TCATGA	1 cut(s)	1933
PceI	AGGCCT	1 cut(s)	716
PciI	ACATGT	1 cut(s)	1354
PdmI	GAANNNNTTC	1 cut(s)	2553
PfeI	GAWTC	9 cut(s)	129, 489, 769, 1225, 1371, 1411, 1715, 2099, 2667
PfoI	TCCNGGA	2 cut(s)	667, 734
PkrI	GCNGC	3 cut(s)	520, 2077, 2310
PleI	GAGTC	8 cut(s)	739, 1499, 1760, 1826, 1962, 2051, 2184, 2784
PpsI	GAGTC	8 cut(s)	739, 1499, 1760, 1826, 1962, 2051, 2184, 2784
PscI	ACATGT	1 cut(s)	1354
PshAI	GACNNNNGTC	1 cut(s)	685
PsiI	TTATAA	4 cut(s)	998, 1604, 2496, 2808
Psp124BI	GAGCTC	1 cut(s)	1513
Psp6I	CCWGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
PspEI	GGTNACC	1 cut(s)	2018
PspFI	CCCAGC	1 cut(s)	1257
PspGI	CCWGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
PspN4I	GGNNCC	4 cut(s)	112, 548, 1379, 1899
PspPI	GGNCC	4 cut(s)	314, 1898, 2341, 2441
PsuI	RGATCY	3 cut(s)	839, 863, 2515
PvuII	CAGCTG	2 cut(s)	755, 1257
RsaI	GTAC	7 cut(s)	422, 603, 1213, 2163, 2216, 2590, 2748
RsaNI	GTAC	7 cut(s)	421, 602, 1212, 2162, 2215, 2589, 2747
RseI	CAYNNNNRTG	2 cut(s)	2222, 2398
SacI	GAGCTC	1 cut(s)	1513
SaqAI	TTAA	7 cut(s)	387, 722, 794, 1095, 1242, 1823, 1944
SatI	GCNGC	3 cut(s)	519, 2076, 2309
Sau96I	GGNCC	4 cut(s)	314, 1898, 2341, 2441
ScaI	AGTACT	1 cut(s)	2748
SchI	GAGTC	8 cut(s)	740, 1500, 1760, 1826, 1962, 2051, 2184, 2784
ScrFI	CCNGG	7 cut(s)	630, 654, 669, 736, 1922, 2777, 2815
SduI	GDGCHC	6 cut(s)	551, 884, 1066, 1120, 1513, 2283
SfaNI	GCATC	8 cut(s)	437, 459, 1044, 1130, 1330, 1915, 2062, 2117
SfcI	CTRYAG	1 cut(s)	2643
SfuI	TTCGAA	1 cut(s)	692
SinI	GGWCC	1 cut(s)	2441
SmiMI	CAYNNNNRTG	2 cut(s)	2222, 2398
SmlI	CTYRAG	2 cut(s)	1907, 2282
SmoI	CTYRAG	2 cut(s)	1907, 2282
SseBI	AGGCCT	1 cut(s)	716
SsiI	CCGC	2 cut(s)	138, 1789
SspI	AATATT	1 cut(s)	802
SspMI	CTAG	6 cut(s)	83, 345, 788, 848, 974, 1403
SstI	GAGCTC	1 cut(s)	1513
StuI	AGGCCT	1 cut(s)	716
StyD4I	CCNGG	7 cut(s)	628, 652, 667, 734, 1920, 2775, 2813
StyI	CCWWGG	4 cut(s)	216, 522, 1761, 2357
TaqI	TCGA	7 cut(s)	173, 246, 256, 692, 1917, 1966, 2547
TaqII	GACCGA	2 cut(s)	1331, 1628
TatI	WGTACW	3 cut(s)	2161, 2214, 2746
TfiI	GAWTC	9 cut(s)	129, 489, 769, 1225, 1371, 1411, 1715, 2099, 2667
Tru1I	TTAA	7 cut(s)	387, 722, 794, 1095, 1242, 1823, 1944
Tru9I	TTAA	7 cut(s)	387, 722, 794, 1095, 1242, 1823, 1944
TseFI	GTSAC	3 cut(s)	982, 1498, 1829
TseI	GCWGC	3 cut(s)	518, 2075, 2308
Tsp45I	GTSAC	3 cut(s)	982, 1498, 1829
TspGWI	ACGGA	2 cut(s)	519, 2576
VneI	GTGCAC	1 cut(s)	1062
VpaK11BI	GGWCC	1 cut(s)	2441
XapI	RAATTY	3 cut(s)	536, 923, 2598
XceI	RCATGY	1 cut(s)	1358
XmiI	GTMKAC	1 cut(s)	87
XmnI	GAANNNNTTC	1 cut(s)	2553
XspI	CTAG	6 cut(s)	83, 345, 788, 848, 974, 1403
ZrmI	AGTACT	1 cut(s)	2748
Zsp2I	ATGCAT	3 cut(s)	452, 1145, 1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.