Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006908

Physical Location & Seq

Genomic Coordinates Reverse (-)

68437809 .. 68442651

Gene Structure 4843 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00131700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGCTAACTTTCTCTTCATCTTACTTGTCATTCGATACATATAATGTCTCTCTTTCATTCTCTACGGCCACTGCTATTTCTGGTTCTCTGCGAATGAGAACTTTCAAAATCCCCACCATTTTTACTTCTAAAAGCCACCCGAGGCTTCCTTACCAAAACCCCATTCGCCAAAATCTAGTAGACAAACCTGAAAGCCAGAATGGCTCAAACAGGGTGTTTAGATTCTTCCGCGGGCTTATGTCCGGAGGGAAAATTGATGGGGGGTCGAGGACAGAGGCAGAGGAGAAGCTTTACTCTTGGTTATACGCCTTGGCGCAATCCGATAAGGATTTGGTTTTCGAGTATGTTCGATCCACCGAAAGAGCCAAGATACAGGAAATGGCTCGGTTCGATTTGATAACTAACTTGCATTGCCTTGCCACATTTTCAGTCTTACATGAAATTCTAGGATTGAGCTTTACTGAAGCTGAAAGGAGATTGAAGGAAAATGGCCCGAATGTTCCTGTTGATTTCTCTTTTCCTAGCTGCGACAGCCCAAATGGATGCATCATGCTTGTTTTGGTTTTGATAAGTGTTTGCCTCCGGTTCTATCAGGAATACGGAAGTTCAAAAGCAGCCATGGAACTTTCAGAATTTGTAAGGTGCCCAGTCAAAGTTCAAAGATGTGCAGGTAGAGTTGTTCAGACTGAATTAGTAGTACAAATTGATCAAAGAGATATTGTTCCTGGTGATATTATCATATTTGAACCTGGAGACCTTTTTCCTGGAGATGTGAGACTATTGTCTTCGAAACACCTTGTTGTAAGTCAGGCCTCATTAACAGGAGAGTCCTGGACAACCGAAAAAACAGCTGATATCAGAGAGAATCAAAGTACTCCATTGCTAGATTTAAGGAATATTTGCTTCATGGGAACTAATGTAGTATCAGGCAGTGGATCTGGTCTAGTGGTTTCTACTGGATCTAAGACATACATGAGCACCATGTTTTCAAACATAGGGAAGAAGAAACCACCAAATGAATTTGAGGACGGTGTTCGTCGCATATCTTATGTGCTGGTTGCTGTTATGCTAGTAGTAGTCACCATCATAGTTATAACTGACTACTCTACATCTCGTGATCTGTCTGAGAGCATCCTTTTTGGAGTGTCAGTTGCAAGTGCACTTACTCCTCAAATGCTTCCCCTGGTCGTTAACACAAGTCTTGCAAAAGGAGCACTTGCTATGGCCAGAGACAGATGCATAATCAAAAGCCTATCTGCAATACGAAACATGGGTTCTATGGATATCTTATGCATTGACAAGACTGGTACACTCACCATGAATCGTGCGATAATGGTTAATTATCTGGACAGCTGGGGGTTAGACAAAGAAAAGGTTTTACAGTTTGCTTTCCTCAACTCATATTTCAAGACCGATCAGAAATATCCTTTGGATGATGCAATTTTGGCACATGTATATACCAATGGATTCAGGTTCCAACCGTCAAAATGGAAGAAACTAGATGAGATTCCTTTTGATTTCATAAGACGAAGGGTATCTATTATCATGGAAAGAGAAGAAGACACAGACCCTCACAGTTTTGTGAGAGTCATGGTGACAAAAGGAGCTCTGGAAGAAGTAATGAAAGTTTGTTCTTTTATGGAGAATGTTGATAGTGGCACGATTTCACCTCTCTCTCCAGAACAGTATCAAAGGATTATAAATATGACTGAGGAAATAAGCAATGAGGGACTAAGAGTTATAGGAGTAGCAACAAAGAAGCTGGAAAAGCAGATAAGGTATGAGCGCAAAGATAATGATGATACTTCCGAATCAGACATGGTTTTCCTCGGCCTCATAACATTCTTTGACCCACCCAAGGACTCAGCAAAGCAAGCTCTGTGGCAGTTGGCTGAGAAGGGAGTGAAAGCAAAAGTATTAACAGGTGACTCACTGTCTCTATCTATAAGAGTTTGCAAGGAAGTTGGTATCAGAACAACTCATGTAGTTACGGGGCCAGAGCTTGAGCTACTCGACCAGGATGCCTTTCATGAGACTGTTAAAACAGCAACGGTCTTAGCTCGACTCACCCCAACGCAGAAACTCCGAGTTGTGCAGTCCTTGCAAACAATTGGTAACCACATTGTTGGATTTTTGGGAGATGGAGTAAATGACTCGCTTGCACTGGATGCAGCCCATGTTGGTATATCAGTTGATTCAGGAGCATCAGTTGCAAAAGACTTTGCTGACATTATCTTACTGGAGAAAGACCTGAATGTACTCATTGCCGGAGTTGAACATGGCCGACTCACTTTTGGGAACACAATGAAGTACATAAAAATGTCAGTTATTGCCAATCTGGGAAGCGTTCTCTCAATTCTCATAGCAACTCTGGTGCTCAAGTATGAGCCATTGACGGCAAGACAGCTTCTTACACAGAACTTCTTGTATAGTGTGGGCCAGATTGCAATCCCATGGGATAAAATGGAAGATGATTATGTAAAAGTCCCACAAAGATGGTCAAAGAAAGGTTTGCCGATGTTCATTTTGTGGAATGGACCTGTCTGCACTCTTTTTGATGTTACTACACTTCTGTTCCTTTGGTTCTATTATAAGGCTGATAGTTTGGAGGATCTTGTTTTCTTCCACACTGCTTGGTTCATCGAAGGGCTTCTCATGCAGACCCTAATCATCCACTTGATTCGTACAGAGAAAATTCCTTTCATTCAGGAGTTTGCCTCATGGCCTGTGCTTTGTTCTACAGTTCTGGTTTCTGCAATTGGAATCGCAATTACATTCACCCCGATTGGGGCAGTGATGGGATTTATCAGGCTTCCACTGTCATACTTTGGGTTTTTGGTAGTACTGTTTATAGGATATTTTGTTGTTGGCCAGGTGGTCAAGAGACTCTACATTTTGGTTTATAAAACCTGGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0090662 GO:0098655 GO:0098660 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

972

Amino Acids

108.85

Weight (kDa)

6.71

Isoelectric Point (pI)

34.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1-E2_ATPase	PF00122	225 - 329	3.4e-17	P-type ATPase actuator domain
Hydrolase	PF00702	429 - 725	4e-08	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	494 - 621	3.7e-23	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	797 - 963	3.1e-18	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006908)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140 FvH4_1g20140
malus_domestica	MD00G1145700.v1.1 MD15G1317200.v1.1
prunus_persica	Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1 Prupe.6G194600_v2.0.a1
pyrus_communis	pycom01g06410
rosa_chinensis	RchiOBHm_Chr2g0112191
rosa_laevigata	RLG00000017957
rosa_multiflora	Rmu_sc0001386.1_g000001 Rmu_sc0026641.1_g000001
rosa_roxburghii	Rroxscaffold_2G00131700
rosa_rugosa	Rorug02G0179000 Rorug02G0179000 Rorug02G0179100
rosa_samantha	Rh2AG231200 Rh2BG244200 Rh2CG235600 Rh2DG239100
rosa_wichuraiana	Rw2G017920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1094, 1700, 2592, 2904
Acc36I	ACCTGC	1 cut(s)	659
AccB1I	GGYRCC	1 cut(s)	642
AccI	GTMKAC	1 cut(s)	180
AccII	CGCG	1 cut(s)	231
AccIII	TCCGGA	1 cut(s)	242
AciI	CCGC	2 cut(s)	229, 231
AclWI	GGATC	4 cut(s)	345, 943, 967, 2619
AcoI	YGGCCR	4 cut(s)	66, 1224, 2281, 2869
AcsI	RAATTY	4 cut(s)	441, 632, 1019, 2694
AcuI	CTGAAG	1 cut(s)	483
AfaI	GTAC	7 cut(s)	699, 874, 1309, 2259, 2312, 2686, 2844
AfiI	CCNNNNNNNGG	3 cut(s)	1858, 2787, 2788
AflIII	ACRYGT	1 cut(s)	1450
AgsI	TTSAA	8 cut(s)	106, 481, 609, 659, 746, 990, 1408, 2276
AhdI	GACNNNNNGTC	1 cut(s)	1933
AjnI	CCWGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
AjuI	GAANNNNNNNTTGG	4 cut(s)	147, 179, 1412, 1444
Alw21I	GWGCWC	5 cut(s)	980, 1162, 1216, 1609, 2379
Alw26I	GTCTC	7 cut(s)	52, 747, 769, 1224, 1941, 2027, 2878
Alw44I	GTGCAC	1 cut(s)	1158
AlwI	GGATC	4 cut(s)	345, 943, 967, 2619
Ama87I	CYCGRG	1 cut(s)	139
Aor13HI	TCCGGA	1 cut(s)	242
ApaLI	GTGCAC	1 cut(s)	1158
ApeKI	GCWGC	3 cut(s)	525, 614, 2171
ApoI	RAATTY	4 cut(s)	441, 632, 1019, 2694
Asp700I	GAANNNNTTC	1 cut(s)	2649
AspLEI	GCGC	2 cut(s)	316, 1788
AspS9I	GGNCC	4 cut(s)	491, 1994, 2437, 2537
AsuHPI	GGTGA	8 cut(s)	740, 1072, 1306, 1606, 1659, 1937, 2059, 2770
AsuII	TTCGAA	1 cut(s)	788
AvaI	CYCGRG	1 cut(s)	139
AvaII	GGWCC	1 cut(s)	2537
BaeGI	GKGCMC	2 cut(s)	647, 1162
BalI	TGGCCA	2 cut(s)	1226, 2871
BanI	GGYRCC	1 cut(s)	642
BanII	GRGCYC	1 cut(s)	1609
BauI	CACGAG	1 cut(s)	1113
BbsI	GAAGAC	2 cut(s)	777, 1566
Bbv12I	GWGCWC	5 cut(s)	980, 1162, 1216, 1609, 2379
BbvI	GCAGC	3 cut(s)	512, 626, 2183
BccI	CCATC	5 cut(s)	251, 1091, 2135, 2490, 2791
BceAI	ACGGC	2 cut(s)	81, 2412
BcgI	CGANNNNNNTGC	2 cut(s)	2003, 2037
BciT130I	CCWGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
BclI	TGATCA	1 cut(s)	706
BcoDI	GTCTC	7 cut(s)	52, 747, 769, 1224, 1941, 2027, 2878
BfaI	CTAG	7 cut(s)	176, 446, 522, 884, 944, 1070, 1499
BfmI	CTRYAG	1 cut(s)	2739
BfuAI	ACCTGC	1 cut(s)	659
BglI	GCCNNNNNGGC	1 cut(s)	201
BisI	GCNGC	3 cut(s)	526, 615, 2172
BlsI	GCNGC	3 cut(s)	527, 616, 2173
BmcAI	AGTACT	2 cut(s)	874, 2844
Bme1390I	CCNGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
Bme18I	GGWCC	1 cut(s)	2537
BmeRI	GACNNNNNGTC	1 cut(s)	1933
BmeT110I	CYCGRG	1 cut(s)	139
BmgT120I	GGNCC	4 cut(s)	491, 1994, 2437, 2537
BmiI	GGNNCC	3 cut(s)	644, 1475, 1995
BmrFI	CCNGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
BmrI	ACTGGG	1 cut(s)	641
BmsI	GCATC	8 cut(s)	533, 555, 1140, 1226, 1426, 2011, 2158, 2213
BmuI	ACTGGG	1 cut(s)	641
BoxI	GACNNNNGTC	1 cut(s)	781
BpiI	GAAGAC	2 cut(s)	777, 1566
BplI	GAGNNNNNCTC	2 cut(s)	2702, 2734
BpmI	CTGGAG	4 cut(s)	771, 786, 1662, 2261
Bpu14I	TTCGAA	1 cut(s)	788
BpuEI	CTTGAG	2 cut(s)	2024, 2363
BsaBI	GATNNNNATC	1 cut(s)	2447
BsaI	GGTCTC	1 cut(s)	747
BsaJI	CCNNGG	8 cut(s)	140, 229, 309, 618, 1182, 1828, 1857, 2453
BsaWI	WCCGGW	2 cut(s)	242, 582
BsaXI	ACNNNNNCTCC	6 cut(s)	1134, 1164, 2130, 2160, 2193, 2223
Bsc4I	CCNNNNNNNGG	3 cut(s)	1858, 2787, 2788
Bse1I	ACTGG	5 cut(s)	647, 961, 1309, 2169, 2244
Bse3DI	GCAATG	4 cut(s)	409, 878, 1729, 2262
Bse8I	GATNNNNATC	1 cut(s)	2447
BseAI	TCCGGA	1 cut(s)	242
BseBI	CCWGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
BseDI	CCNNGG	8 cut(s)	140, 229, 309, 618, 1182, 1828, 1857, 2453
BseGI	GGATG	6 cut(s)	548, 1131, 1438, 2026, 2173, 2670
BseJI	GATNNNNATC	1 cut(s)	2447
BseLI	CCNNNNNNNGG	3 cut(s)	1858, 2787, 2788
BseMI	GCAATG	4 cut(s)	409, 878, 1729, 2262
BseMII	CTCAG	4 cut(s)	1116, 1701, 1878, 1884
BseNI	ACTGG	5 cut(s)	647, 961, 1309, 2169, 2244
BseRI	GAGGAG	2 cut(s)	296, 1158
BseSI	GKGCMC	2 cut(s)	647, 1162
BseXI	GCAGC	3 cut(s)	512, 626, 2183
BseYI	CCCAGC	1 cut(s)	1353
BsgI	GTGCAG	3 cut(s)	687, 2114, 2530
Bsh1236I	CGCG	1 cut(s)	231
BshNI	GGYRCC	1 cut(s)	642
BsiHKAI	GWGCWC	5 cut(s)	980, 1162, 1216, 1609, 2379
BsiHKCI	CYCGRG	1 cut(s)	139
BsiSI	CCGG	3 cut(s)	243, 583, 2268
BslFI	GGGAC	2 cut(s)	1743, 2471
BslI	CCNNNNNNNGG	3 cut(s)	1858, 2787, 2788
BsmAI	GTCTC	7 cut(s)	52, 747, 769, 1224, 1941, 2027, 2878
BsmFI	GGGAC	2 cut(s)	1743, 2471
Bso31I	GGTCTC	1 cut(s)	747
BsoBI	CYCGRG	1 cut(s)	139
Bsp119I	TTCGAA	1 cut(s)	788
Bsp1286I	GDGCHC	6 cut(s)	647, 980, 1162, 1216, 1609, 2379
Bsp13I	TCCGGA	1 cut(s)	242
Bsp143I	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
Bsp19I	CCATGG	2 cut(s)	618, 2453
BspACI	CCGC	2 cut(s)	229, 231
BspCNI	CTCAG	4 cut(s)	1117, 1702, 1877, 1885
BspEI	TCCGGA	1 cut(s)	242
BspFNI	CGCG	1 cut(s)	231
BspHI	TCATGA	1 cut(s)	2029
BspLI	GGNNCC	3 cut(s)	644, 1475, 1995
BspMI	ACCTGC	1 cut(s)	659
BspPI	GGATC	4 cut(s)	345, 943, 967, 2619
BspT104I	TTCGAA	1 cut(s)	788
BspT107I	GGYRCC	1 cut(s)	642
BspTNI	GGTCTC	1 cut(s)	747
BsrDI	GCAATG	4 cut(s)	409, 878, 1729, 2262
BsrI	ACTGG	5 cut(s)	647, 961, 1309, 2169, 2244
BssECI	CCNNGG	8 cut(s)	140, 229, 309, 618, 1182, 1828, 1857, 2453
BssMI	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
BssSI	CACGAG	1 cut(s)	1113
BssT1I	CCWWGG	4 cut(s)	309, 618, 1857, 2453
Bst2BI	CACGAG	1 cut(s)	1113
Bst2UI	CCWGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
Bst6I	CTCTTC	1 cut(s)	19
BstAPI	GCANNNNNTGC	3 cut(s)	2101, 2168, 2210
BstBI	TTCGAA	1 cut(s)	788
BstC8I	GCNNGC	3 cut(s)	233, 1875, 2160
BstDEI	CTNAG	7 cut(s)	963, 1125, 1710, 1733, 1864, 1893, 2056
BstDSI	CCRYGG	3 cut(s)	229, 618, 2453
BstEII	GGTNACC	1 cut(s)	2114
BstF5I	GGATG	6 cut(s)	548, 1131, 1438, 2026, 2173, 2670
BstFNI	CGCG	1 cut(s)	231
BstHHI	GCGC	2 cut(s)	316, 1788
BstKTI	GATC	7 cut(s)	353, 709, 938, 962, 1120, 1417, 2614
BstMAI	GTCTC	7 cut(s)	52, 747, 769, 1224, 1941, 2027, 2878
BstMBI	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
BstNI	CCWGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
BstNSI	RCATGY	1 cut(s)	1454
BstPAI	GACNNNNGTC	1 cut(s)	781
BstPI	GGTNACC	1 cut(s)	2114
BstSCI	CCNGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
BstSFI	CTRYAG	1 cut(s)	2739
BstSLI	GKGCMC	2 cut(s)	647, 1162
BstUI	CGCG	1 cut(s)	231
BstV1I	GCAGC	3 cut(s)	512, 626, 2183
BstV2I	GAAGAC	2 cut(s)	777, 1566
BstX2I	RGATCY	3 cut(s)	935, 959, 2611
BstXI	CCANNNNNNTGG	2 cut(s)	2126, 2496
BstYI	RGATCY	3 cut(s)	935, 959, 2611
BtgI	CCRYGG	3 cut(s)	229, 618, 2453
BtsCI	GGATG	6 cut(s)	548, 1131, 1438, 2026, 2173, 2670
BtsI	GCAGTG	4 cut(s)	69, 937, 2628, 2799
BtsIMutI	CAGTG	7 cut(s)	69, 937, 1931, 2162, 2628, 2799, 2816
BveI	ACCTGC	1 cut(s)	659
Cac8I	GCNNGC	3 cut(s)	233, 1875, 2160
CciI	TCATGA	1 cut(s)	2029
CfoI	GCGC	2 cut(s)	316, 1788
Cfr13I	GGNCC	4 cut(s)	491, 1994, 2437, 2537
Cfr42I	CCGCGG	1 cut(s)	232
Csp6I	GTAC	7 cut(s)	698, 873, 1308, 2258, 2311, 2685, 2843
CspCI	CAANNNNNGTGG	4 cut(s)	1862, 1897, 2615, 2650
CviQI	GTAC	7 cut(s)	698, 873, 1308, 2258, 2311, 2685, 2843
DdeI	CTNAG	7 cut(s)	963, 1125, 1710, 1733, 1864, 1893, 2056
DpnI	GATC	7 cut(s)	352, 708, 937, 961, 1119, 1416, 2613
DpnII	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
DriI	GACNNNNNGTC	1 cut(s)	1933
EaeI	YGGCCR	4 cut(s)	66, 1224, 2281, 2869
Eam1104I	CTCTTC	1 cut(s)	19
Eam1105I	GACNNNNNGTC	1 cut(s)	1933
EarI	CTCTTC	1 cut(s)	19
Ecl136II	GAGCTC	1 cut(s)	1607
Eco130I	CCWWGG	4 cut(s)	309, 618, 1857, 2453
Eco147I	AGGCCT	1 cut(s)	812
Eco24I	GRGCYC	1 cut(s)	1609
Eco31I	GGTCTC	1 cut(s)	747
Eco32I	GATATC	2 cut(s)	856, 1285
Eco47I	GGWCC	1 cut(s)	2537
Eco53kI	GAGCTC	1 cut(s)	1607
Eco57I	CTGAAG	1 cut(s)	483
Eco88I	CYCGRG	1 cut(s)	139
Eco91I	GGTNACC	1 cut(s)	2114
EcoICRI	GAGCTC	1 cut(s)	1607
EcoO65I	GGTNACC	1 cut(s)	2114
EcoRII	CCWGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
EcoRV	GATATC	2 cut(s)	856, 1285
EcoT14I	CCWWGG	4 cut(s)	309, 618, 1857, 2453
EcoT22I	ATGCAT	3 cut(s)	548, 1241, 1295
EcoT38I	GRGCYC	1 cut(s)	1609
ErhI	CCWWGG	4 cut(s)	309, 618, 1857, 2453
FalI	AAGNNNNNCTT	2 cut(s)	1200, 1232
FaqI	GGGAC	2 cut(s)	1743, 2471
FauI	CCCGC	1 cut(s)	224
FbaI	TGATCA	1 cut(s)	706
FblI	GTMKAC	1 cut(s)	180
Fnu4HI	GCNGC	3 cut(s)	526, 615, 2172
FokI	GGATG	6 cut(s)	555, 1118, 1445, 2033, 2180, 2657
FriOI	GRGCYC	1 cut(s)	1609
Fsp4HI	GCNGC	3 cut(s)	526, 615, 2172
FspBI	CTAG	7 cut(s)	176, 446, 522, 884, 944, 1070, 1499
GlaI	GCGC	2 cut(s)	315, 1787
GluI	GCNGC	3 cut(s)	526, 615, 2172
GsaI	CCCAGC	1 cut(s)	1357
GsuI	CTGGAG	4 cut(s)	771, 786, 1662, 2261
HapII	CCGG	3 cut(s)	243, 583, 2268
HhaI	GCGC	2 cut(s)	316, 1788
Hin6I	GCGC	2 cut(s)	314, 1786
HinP1I	GCGC	2 cut(s)	314, 1786
HincII	GTYRAC	1 cut(s)	1192
HindII	GTYRAC	1 cut(s)	1192
HindIII	AAGCTT	1 cut(s)	287
HpaI	GTTAAC	1 cut(s)	1192
HpaII	CCGG	3 cut(s)	243, 583, 2268
HphI	GGTGA	8 cut(s)	740, 1072, 1306, 1606, 1659, 1937, 2059, 2770
Hpy166II	GTNNAC	4 cut(s)	181, 1160, 1192, 1310
Hpy8I	GTNNAC	4 cut(s)	181, 1160, 1192, 1310
Hpy99I	CGWCG	1 cut(s)	1041
HpyAV	CCTTC	4 cut(s)	475, 1524, 1891, 2639
HpyF3I	CTNAG	7 cut(s)	963, 1125, 1710, 1733, 1864, 1893, 2056
HspAI	GCGC	2 cut(s)	314, 1786
Kpn2I	TCCGGA	1 cut(s)	242
Ksp22I	TGATCA	1 cut(s)	706
KspAI	GTTAAC	1 cut(s)	1192
KspI	CCGCGG	1 cut(s)	232
Kzo9I	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
LmnI	GCTCC	3 cut(s)	1211, 1604, 2201
Lsp1109I	GCAGC	3 cut(s)	512, 626, 2183
LweI	GCATC	8 cut(s)	533, 555, 1140, 1226, 1426, 2011, 2158, 2213
MaeI	CTAG	7 cut(s)	176, 446, 522, 884, 944, 1070, 1499
MaeIII	GTNAC	6 cut(s)	1078, 1594, 1925, 1987, 2114, 2560
MalI	GATC	7 cut(s)	352, 708, 937, 961, 1119, 1416, 2613
MboI	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
MfeI	CAATTG	2 cut(s)	2109, 2757
MflI	RGATCY	3 cut(s)	935, 959, 2611
MhlI	GDGCHC	6 cut(s)	647, 980, 1162, 1216, 1609, 2379
MlsI	TGGCCA	2 cut(s)	1226, 2871
MluNI	TGGCCA	2 cut(s)	1226, 2871
MlyI	GAGTC	8 cut(s)	836, 1596, 1856, 1922, 2058, 2147, 2280, 2880
MmeI	TCCRAC	2 cut(s)	1501, 2107
Mox20I	TGGCCA	2 cut(s)	1226, 2871
Mph1103I	ATGCAT	3 cut(s)	548, 1241, 1295
MroI	TCCGGA	1 cut(s)	242
MroXI	GAANNNNTTC	1 cut(s)	2649
MscI	TGGCCA	2 cut(s)	1226, 2871
MseI	TTAA	6 cut(s)	818, 890, 1191, 1338, 1919, 2040
MslI	CAYNNNNRTG	2 cut(s)	2318, 2494
Msp20I	TGGCCA	2 cut(s)	1226, 2871
MspA1I	CMGCKG	3 cut(s)	231, 851, 1353
MspI	CCGG	3 cut(s)	243, 583, 2268
MspR9I	CCNGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
MunI	CAATTG	2 cut(s)	2109, 2757
MvaI	CCWGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
MvnI	CGCG	1 cut(s)	231
NcoI	CCATGG	2 cut(s)	618, 2453
NdeII	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
NlaIV	GGNNCC	3 cut(s)	644, 1475, 1995
NmeAIII	GCCGAG	1 cut(s)	1809
NmuCI	GTSAC	3 cut(s)	1078, 1594, 1925
NsiI	ATGCAT	3 cut(s)	548, 1241, 1295
NspI	RCATGY	1 cut(s)	1454
NspV	TTCGAA	1 cut(s)	788
PagI	TCATGA	1 cut(s)	2029
PceI	AGGCCT	1 cut(s)	812
PciI	ACATGT	1 cut(s)	1450
PdmI	GAANNNNTTC	1 cut(s)	2649
PfeI	GAWTC	9 cut(s)	222, 865, 1321, 1467, 1507, 1811, 2195, 2680, 2763
PfoI	TCCNGGA	2 cut(s)	763, 830
PkrI	GCNGC	3 cut(s)	527, 616, 2173
PleI	GAGTC	8 cut(s)	835, 1595, 1856, 1922, 2058, 2147, 2280, 2880
PpsI	GAGTC	8 cut(s)	835, 1595, 1856, 1922, 2058, 2147, 2280, 2880
PscI	ACATGT	1 cut(s)	1450
PshAI	GACNNNNGTC	1 cut(s)	781
PsiI	TTATAA	4 cut(s)	1094, 1700, 2592, 2904
Psp124BI	GAGCTC	1 cut(s)	1609
Psp6I	CCWGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
PspEI	GGTNACC	1 cut(s)	2114
PspFI	CCCAGC	1 cut(s)	1353
PspGI	CCWGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
PspN4I	GGNNCC	3 cut(s)	644, 1475, 1995
PspPI	GGNCC	4 cut(s)	491, 1994, 2437, 2537
PsuI	RGATCY	3 cut(s)	935, 959, 2611
PvuII	CAGCTG	2 cut(s)	851, 1353
RsaI	GTAC	7 cut(s)	699, 874, 1309, 2259, 2312, 2686, 2844
RsaNI	GTAC	7 cut(s)	698, 873, 1308, 2258, 2311, 2685, 2843
RseI	CAYNNNNRTG	2 cut(s)	2318, 2494
SacI	GAGCTC	1 cut(s)	1609
SacII	CCGCGG	1 cut(s)	232
SaqAI	TTAA	6 cut(s)	818, 890, 1191, 1338, 1919, 2040
SatI	GCNGC	3 cut(s)	526, 615, 2172
Sau3AI	GATC	7 cut(s)	350, 706, 935, 959, 1117, 1414, 2611
Sau96I	GGNCC	4 cut(s)	491, 1994, 2437, 2537
ScaI	AGTACT	2 cut(s)	874, 2844
SchI	GAGTC	8 cut(s)	836, 1596, 1856, 1922, 2058, 2147, 2280, 2880
ScrFI	CCNGG	8 cut(s)	726, 750, 765, 832, 1184, 2018, 2873, 2911
SduI	GDGCHC	6 cut(s)	647, 980, 1162, 1216, 1609, 2379
SfaNI	GCATC	8 cut(s)	533, 555, 1140, 1226, 1426, 2011, 2158, 2213
SfcI	CTRYAG	1 cut(s)	2739
Sfr303I	CCGCGG	1 cut(s)	232
SfuI	TTCGAA	1 cut(s)	788
SgrBI	CCGCGG	1 cut(s)	232
SinI	GGWCC	1 cut(s)	2537
SmiMI	CAYNNNNRTG	2 cut(s)	2318, 2494
SmlI	CTYRAG	2 cut(s)	2003, 2378
SmoI	CTYRAG	2 cut(s)	2003, 2378
SseBI	AGGCCT	1 cut(s)	812
SsiI	CCGC	2 cut(s)	229, 231
SspI	AATATT	1 cut(s)	898
SspMI	CTAG	7 cut(s)	176, 446, 522, 884, 944, 1070, 1499
SstI	GAGCTC	1 cut(s)	1609
StuI	AGGCCT	1 cut(s)	812
StyD4I	CCNGG	8 cut(s)	724, 748, 763, 830, 1182, 2016, 2871, 2909
StyI	CCWWGG	4 cut(s)	309, 618, 1857, 2453
TaqI	TCGA	9 cut(s)	33, 266, 339, 349, 390, 788, 2013, 2062, 2643
TaqII	GACCGA	1 cut(s)	1427
TatI	WGTACW	5 cut(s)	697, 872, 2257, 2310, 2842
TfiI	GAWTC	9 cut(s)	222, 865, 1321, 1467, 1507, 1811, 2195, 2680, 2763
Tru1I	TTAA	6 cut(s)	818, 890, 1191, 1338, 1919, 2040
Tru9I	TTAA	6 cut(s)	818, 890, 1191, 1338, 1919, 2040
TscAI	CASTG	7 cut(s)	76, 937, 1938, 2169, 2635, 2799, 2823
TseFI	GTSAC	3 cut(s)	1078, 1594, 1925
TseI	GCWGC	3 cut(s)	525, 614, 2171
Tsp45I	GTSAC	3 cut(s)	1078, 1594, 1925
TspGWI	ACGGA	1 cut(s)	615
TspRI	CASTG	7 cut(s)	76, 937, 1938, 2169, 2635, 2799, 2823
VneI	GTGCAC	1 cut(s)	1158
VpaK11BI	GGWCC	1 cut(s)	2537
XapI	RAATTY	4 cut(s)	441, 632, 1019, 2694
XceI	RCATGY	1 cut(s)	1454
XmiI	GTMKAC	1 cut(s)	180
XmnI	GAANNNNTTC	1 cut(s)	2649
XspI	CTAG	7 cut(s)	176, 446, 522, 884, 944, 1070, 1499
ZrmI	AGTACT	2 cut(s)	874, 2844
Zsp2I	ATGCAT	3 cut(s)	548, 1241, 1295

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.