FvH4_1g20260

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009505
Physical Location & Seq
Forward (+)
12501904 .. 12507538
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20260.t3

Sequence Viewer

Length: 1809 bp
ATGTCAGACACCGACGTTGTTTCCGGCACGTTCCGGGCGCTGGTGGAGAGCGCGGACCGGAAGTTCGCGCGTGTCCGCGACGTGCCGGCGTACGGGCGCGTGCACAACCAGCACTACTTCCACAAGGTGTTCAAGGCGTACATGCGTCTCTGGAAGTACCAGCAGGAGAACCGGGCGAAGCTGGTGGAGGCCGGTCTGAACCGGTGGGAAATCGGCGAGATCGCGAGCCGGATCGGCCAGCTGTACTTCGGTCAGTACATGAGGACGAGCGAGGCGAGGTTTCTGGCGGAGGCGTACGTGTTCTACGAGGCGATTCTGAGCCGGAGGTACTTCGAGGGAGGAGCCAAGGGTTTGGGGAAGAAGGAGATTGGAGTCCGGTTCAAGGAGCTGAGATTTTACGCGAGGTTTTTGCTGGTGGCGTTGATTTTGAACCGGACCGAGACGGTTCAGCTGCTCGCCGAGCGGTTCAAGGCTCTGGTTGATGACAGCAGAGCCAATTACCGGGAAACTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGTGCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCATTGCGATATTCTGCCATGTTTGATTCACATCCGGCATCTCTCCCATATGTGGCTCGTTTTCATGCAAAGAGGGTTCTTAAATTTCAAGACGCACTGCTGACAAGCTATCACCGGAACGAGGCCAAGTTTGCTGAACTTACATTGGACACATATCGAATGCTGCAATGTTTGGAATGGGAGCCTAGTGGGTCTTTCTACCATAAGCGCCCTGTTGAATCAAAAGAGAATGGCTCTTTTATTGAGCACTCTGGGGCTTCTGCGGCTTCTGGGGTCATTGATATGAACTTGGCTGCAGATATGACTGATCCAAGTTTACCTCCAAATCCAAGGAAAGCAGTTATTTATCGTCCCTCGGTGACACATTTGATAGCGGTGATGGCTACAATATGTGAGGAACTCCCTGTGGACAGTGTCATGCTCCTGTACCTGTCAGCCTCAGGGACGGCTGGTCGTAGTAATGTTAATCAGCTTTACACCTCTGGAGGATCACAGAAATCGTCAAAAAATAAAGTTCCTCTCCCAAATCAGAATACGTATCGGGCTGAATCTTGTAATGGCAAGGCAGAATCAAGTGGCTATTATGATAAGTATCTGTGGTTTGGTCCTAGAGGGAATGGCGGTGAGAATAACCTATACCCTGGAGACATAATTCCTTTTACTCGCAGACCTCTTTTCTTGATAGTTGATAGTGACAACAGCCATGCATTCAAGGTTCTACATGGTGCGGAAAGGGGAGAGACAGCTGCTCTGTTTCTTTCACCTTTGAGACCAGCATTCAAGAATCCAGCTGATGCTGATTTAACGCAAAACGGAAGTCAGTTCACCTTTTTCTTGACTGCCCCTCTCTCAGCATTCTGCCAATTAGTTGGTCTCTCCTCTTCTGATACTGAAACAGACGTCTATAACGGTGCTGAGGGCATACTTTCTGATGCCTTCTCTAAGTGGGAAATAATACTCTGTACATCAACAAAGATGGATCTGGTGTGGGCCCAAGTTATGTCTGATCCATTTCTACGGCGACTTATTCTAAGATTTATATTCTGCAGAGCTGTGCTTTCGCTCTTCTGTCCTCCAGAAGATAGTGAACAATATTTGCCAGTTTGCCTACCCTTTCTTCCCAGTTCTGTCTCTCCAGATTCTGAAGTAGTACAGTCTTGTATCTCCAGACTTGCAAAGCACCTCAGAGTAGATGACTGTTTTAACTTTGATGACACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

603

Amino Acids

68.27

Weight (kDa)

8.5

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 11 - 396 2.2e-122 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1491
AccBSI CCGCTC 1 cut(s) 463
AccII CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
AciI CCGC 8 cut(s) 53, 76, 287, 463, 851, 962, 1209, 1316
AclWI GGATC 5 cut(s) 239, 890, 1084, 1575, 1589
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 1 cut(s) 1752
AcvI CACGTG 1 cut(s) 1806
AcyI GRCGYC 1 cut(s) 1488
AdeI CACNNNGTG 1 cut(s) 127
AfiI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
AflIII ACRYGT 2 cut(s) 297, 1803
AgeI ACCGGT 1 cut(s) 201
AgsI TTSAA 8 cut(s) 133, 382, 430, 469, 677, 806, 1300, 1369
AhdI GACNNNNNGTC 1 cut(s) 1038
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 1228
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 9 cut(s) 181, 241, 388, 451, 696, 1060, 1334, 1379, 1640
AluI AGCT 9 cut(s) 181, 241, 388, 451, 696, 1060, 1334, 1379, 1640
Alw21I GWGCWC 2 cut(s) 105, 837
Alw26I GTCTC 7 cut(s) 152, 434, 1227, 1322, 1351, 1466, 1723
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 5 cut(s) 239, 890, 1084, 1575, 1589
AlwNI CAGNNNCTG 1 cut(s) 1730
AoxI GGCC 6 cut(s) 189, 235, 570, 587, 711, 1578
ApaI GGGCCC 1 cut(s) 1582
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 4 cut(s) 451, 751, 881, 1334
ApoI RAATTY 1 cut(s) 671
ArsI GACNNNNNNTTYG 4 cut(s) 47, 79, 1390, 1422
AsiGI ACCGGT 1 cut(s) 201
AspLEI GCGC 5 cut(s) 40, 53, 70, 99, 798
AspS9I GGNCC 5 cut(s) 55, 435, 1193, 1578, 1579
AsuC2I CCSGG 3 cut(s) 35, 173, 503
AsuHPI GGTGA 6 cut(s) 692, 958, 976, 1223, 1341, 1405
AvaII GGWCC 3 cut(s) 55, 435, 1193
AxyI CCTNAGG 1 cut(s) 1027
BaeGI GKGCMC 2 cut(s) 105, 1582
BanII GRGCYC 1 cut(s) 1582
BbrPI CACGTG 1 cut(s) 1806
Bbv12I GWGCWC 2 cut(s) 105, 837
BbvCI CCTCAGC 1 cut(s) 1503
BbvI GCAGC 4 cut(s) 438, 738, 868, 1321
BccI CCATC 2 cut(s) 961, 1558
BceAI ACGGC 3 cut(s) 585, 1050, 1622
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciT130I CCWGG 1 cut(s) 1230
BcnI CCSGG 3 cut(s) 35, 173, 503
BcoDI GTCTC 7 cut(s) 152, 434, 1227, 1322, 1351, 1466, 1723
BfaI CTAG 3 cut(s) 530, 774, 1197
BfmI CTRYAG 2 cut(s) 882, 1633
BfoI RGCGCY 2 cut(s) 41, 799
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 452, 752, 852, 882, 1335
BlsI GCNGC 5 cut(s) 453, 753, 853, 883, 1336
Bme1390I CCNGG 4 cut(s) 35, 173, 503, 1230
Bme18I GGWCC 3 cut(s) 55, 435, 1193
BmeRI GACNNNNNGTC 1 cut(s) 1038
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 5 cut(s) 55, 435, 1193, 1578, 1579
BmiI GGNNCC 3 cut(s) 343, 771, 1580
BmrFI CCNGG 4 cut(s) 35, 173, 503, 1230
BmrI ACTGGG 1 cut(s) 1704
BmsI GCATC 3 cut(s) 635, 1372, 1510
BmuI ACTGGG 1 cut(s) 1704
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpmI CTGGAG 5 cut(s) 1092, 1251, 1647, 1707, 1738
Bpu10I CCTNAGC 1 cut(s) 1503
BpuMI CCSGG 3 cut(s) 35, 173, 503
BsaAI YACGTR 3 cut(s) 298, 1125, 1806
BsaBI GATNNNNATC 2 cut(s) 618, 1179
BsaHI GRCGYC 1 cut(s) 1488
BsaI GGTCTC 2 cut(s) 1351, 1466
BsaJI CCNNGG 4 cut(s) 345, 917, 942, 1228
BsaWI WCCGGW 5 cut(s) 57, 201, 375, 432, 702
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
Bse118I RCCGGY 3 cut(s) 85, 191, 201
Bse1I ACTGG 2 cut(s) 1688, 1710
Bse21I CCTNAGG 1 cut(s) 1027
Bse3DI GCAATG 2 cut(s) 590, 761
Bse8I GATNNNNATC 2 cut(s) 618, 1179
BseBI CCWGG 1 cut(s) 1230
BseDI CCNNGG 4 cut(s) 345, 917, 942, 1228
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 2 cut(s) 618, 1179
BseLI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
BseMI GCAATG 2 cut(s) 590, 761
BseMII CTCAG 6 cut(s) 308, 380, 1041, 1452, 1494, 1786
BseNI ACTGG 2 cut(s) 1688, 1710
BseRI GAGGAG 2 cut(s) 354, 1456
BseSI GKGCMC 2 cut(s) 105, 1582
BseXI GCAGC 4 cut(s) 438, 738, 868, 1321
Bsh1236I CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
BshFI GGCC 6 cut(s) 191, 237, 572, 589, 713, 1580
BshTI ACCGGT 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 105, 837
BsiWI CGTACG 2 cut(s) 90, 294
BslFI GGGAC 2 cut(s) 924, 1045
BslI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
BsmAI GTCTC 7 cut(s) 152, 434, 1227, 1322, 1351, 1466, 1723
BsmBI CGTCTC 2 cut(s) 152, 434
BsmFI GGGAC 2 cut(s) 924, 1045
BsmI GAATGC 4 cut(s) 753, 1295, 1364, 1442
BsnI GGCC 6 cut(s) 191, 237, 572, 589, 713, 1580
Bso31I GGTCTC 2 cut(s) 1351, 1466
Bsp120I GGGCCC 1 cut(s) 1578
Bsp1286I GDGCHC 3 cut(s) 105, 837, 1582
Bsp1407I TGTACA 1 cut(s) 1550
Bsp143I GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
Bsp68I TCGCGA 1 cut(s) 224
BspACI CCGC 8 cut(s) 53, 76, 287, 463, 851, 962, 1209, 1316
BspANI GGCC 6 cut(s) 191, 237, 572, 589, 713, 1580
BspCNI CTCAG 6 cut(s) 309, 381, 1040, 1451, 1495, 1785
BspFNI CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
BspLI GGNNCC 3 cut(s) 343, 771, 1580
BspMAI CTGCAG 2 cut(s) 886, 1637
BspPI GGATC 5 cut(s) 239, 890, 1084, 1575, 1589
BspQI GCTCTTC 1 cut(s) 1658
BspTNI GGTCTC 2 cut(s) 1351, 1466
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 2 cut(s) 590, 761
BsrFI RCCGGY 3 cut(s) 85, 191, 201
BsrGI TGTACA 1 cut(s) 1550
BsrI ACTGG 2 cut(s) 1688, 1710
BssAI RCCGGY 3 cut(s) 85, 191, 201
BssECI CCNNGG 4 cut(s) 345, 917, 942, 1228
BssMI GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
BssNI GRCGYC 1 cut(s) 1488
BssT1I CCWWGG 2 cut(s) 345, 917
Bst2UI CCWGG 1 cut(s) 1230
Bst4CI ACNGT 5 cut(s) 445, 1001, 1499, 1743, 1787
Bst6I CTCTTC 2 cut(s) 1474, 1658
BstACI GRCGYC 1 cut(s) 1488
BstAUI TGTACA 1 cut(s) 1550
BstBAI YACGTR 3 cut(s) 298, 1125, 1806
BstC8I GCNNGC 5 cut(s) 87, 101, 226, 239, 456
BstDEI CTNAG 8 cut(s) 317, 389, 1027, 1438, 1503, 1530, 1619, 1772
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
BstH2I RGCGCY 2 cut(s) 41, 799
BstHHI GCGC 5 cut(s) 40, 53, 70, 99, 798
BstKTI GATC 6 cut(s) 222, 234, 898, 1079, 1570, 1597
BstMAI GTCTC 7 cut(s) 152, 434, 1227, 1322, 1351, 1466, 1723
BstMBI GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
BstMWI GCNNNNNNNGC 7 cut(s) 109, 234, 460, 535, 719, 851, 968
BstNI CCWGG 1 cut(s) 1230
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 4 cut(s) 33, 171, 501, 1228
BstSFI CTRYAG 2 cut(s) 882, 1633
BstSLI GKGCMC 2 cut(s) 105, 1582
BstSNI TACGTA 1 cut(s) 1125
BstUI CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
BstV1I GCAGC 4 cut(s) 438, 738, 868, 1321
BstX2I RGATCY 1 cut(s) 1567
BstXI CCANNNNNNTGG 2 cut(s) 352, 1457
BstYI RGATCY 1 cut(s) 1567
Bsu36I CCTNAGG 1 cut(s) 1027
BsuRI GGCC 6 cut(s) 191, 237, 572, 589, 713, 1580
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 683
BtsIMutI CAGTG 2 cut(s) 683, 1006
BtuMI TCGCGA 1 cut(s) 224
Cac8I GCNNGC 5 cut(s) 87, 101, 226, 239, 456
CaiI CAGNNNCTG 1 cut(s) 1730
CfoI GCGC 5 cut(s) 40, 53, 70, 99, 798
Cfr10I RCCGGY 3 cut(s) 85, 191, 201
Cfr13I GGNCC 5 cut(s) 55, 435, 1193, 1578, 1579
CpoI CGGWCCG 2 cut(s) 55, 435
CseI GACGC 2 cut(s) 134, 689
CspAI ACCGGT 1 cut(s) 201
CspI CGGWCCG 2 cut(s) 55, 435
CviAII CATG 7 cut(s) 142, 259, 607, 653, 1006, 1292, 1310
DdeI CTNAG 8 cut(s) 317, 389, 1027, 1438, 1503, 1530, 1619, 1772
DpnI GATC 6 cut(s) 221, 233, 897, 1078, 1569, 1596
DpnII GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
DraI TTTAAA 1 cut(s) 517
DraIII CACNNNGTG 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 1038
EaeI YGGCCR 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 1474, 1658
Eam1105I GACNNNNNGTC 1 cut(s) 1038
EarI CTCTTC 2 cut(s) 1474, 1658
EciI GGCGGA 1 cut(s) 302
Eco105I TACGTA 1 cut(s) 1125
Eco130I CCWWGG 2 cut(s) 345, 917
Eco24I GRGCYC 1 cut(s) 1582
Eco31I GGTCTC 2 cut(s) 1351, 1466
Eco47I GGWCC 3 cut(s) 55, 435, 1193
Eco57I CTGAAG 1 cut(s) 1752
Eco72I CACGTG 1 cut(s) 1806
Eco81I CCTNAGG 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 1228
EcoT14I CCWWGG 2 cut(s) 345, 917
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 1582
ErhI CCWWGG 2 cut(s) 345, 917
Esp3I CGTCTC 2 cut(s) 152, 434
FaeI CATG 7 cut(s) 145, 262, 610, 656, 1009, 1295, 1313
FaqI GGGAC 2 cut(s) 924, 1045
FatI CATG 7 cut(s) 141, 258, 606, 652, 1005, 1291, 1309
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 5 cut(s) 452, 752, 852, 882, 1335
FokI GGATG 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 1582
Fsp4HI GCNGC 5 cut(s) 452, 752, 852, 882, 1335
FspBI CTAG 3 cut(s) 530, 774, 1197
GlaI GCGC 5 cut(s) 39, 52, 69, 98, 797
GluI GCNGC 5 cut(s) 452, 752, 852, 882, 1335
GsuI CTGGAG 5 cut(s) 1092, 1251, 1647, 1707, 1738
HaeII RGCGCY 2 cut(s) 41, 799
HaeIII GGCC 6 cut(s) 191, 237, 572, 589, 713, 1580
HgaI GACGC 2 cut(s) 134, 689
HhaI GCGC 5 cut(s) 40, 53, 70, 99, 798
Hin1I GRCGYC 1 cut(s) 1488
Hin1II CATG 7 cut(s) 145, 262, 610, 656, 1009, 1295, 1313
Hin6I GCGC 5 cut(s) 38, 51, 68, 97, 796
HinP1I GCGC 5 cut(s) 38, 51, 68, 97, 796
HinfI GANTC 8 cut(s) 313, 372, 614, 806, 1136, 1157, 1372, 1727
HphI GGTGA 6 cut(s) 692, 958, 976, 1223, 1341, 1405
Hpy166II GTNNAC 5 cut(s) 103, 905, 997, 1413, 1676
Hpy188I TCNGA 9 cut(s) 7, 198, 318, 1119, 1474, 1519, 1594, 1732, 1775
Hpy8I GTNNAC 5 cut(s) 103, 905, 997, 1413, 1676
Hpy99I CGWCG 2 cut(s) 17, 83
HpyAV CCTTC 2 cut(s) 355, 1534
HpyCH4III ACNGT 5 cut(s) 445, 1001, 1499, 1743, 1787
HpyCH4IV ACGT 7 cut(s) 15, 29, 81, 297, 1124, 1488, 1805
HpyCH4V TGCA 8 cut(s) 103, 538, 656, 754, 884, 1295, 1635, 1763
HpyF10VI GCNNNNNNNGC 7 cut(s) 109, 234, 460, 535, 719, 851, 968
HpyF3I CTNAG 8 cut(s) 317, 389, 1027, 1438, 1503, 1530, 1619, 1772
HpySE526I ACGT 7 cut(s) 15, 29, 81, 297, 1124, 1488, 1805
Hsp92I GRCGYC 1 cut(s) 1488
Hsp92II CATG 7 cut(s) 145, 262, 610, 656, 1009, 1295, 1313
HspAI GCGC 5 cut(s) 38, 51, 68, 97, 796
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Kzo9I GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
LguI GCTCTTC 1 cut(s) 1658
LmnI GCTCC 4 cut(s) 341, 385, 769, 1014
Lsp1109I GCAGC 4 cut(s) 438, 738, 868, 1321
LweI GCATC 3 cut(s) 635, 1372, 1510
MaeI CTAG 3 cut(s) 530, 774, 1197
MaeII ACGT 7 cut(s) 15, 29, 81, 297, 1124, 1488, 1805
MaeIII GTNAC 2 cut(s) 946, 1280
MalI GATC 6 cut(s) 221, 233, 897, 1078, 1569, 1596
MbiI CCGCTC 1 cut(s) 463
MboI GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
MboII GAAGA 5 cut(s) 370, 1461, 1645, 1679, 1697
MflI RGATCY 1 cut(s) 1567
MhlI GDGCHC 3 cut(s) 105, 837, 1582
MluCI AATT 6 cut(s) 496, 511, 543, 671, 1239, 1451
MlyI GAGTC 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 1297
MroNI GCCGGC 1 cut(s) 85
MseI TTAA 5 cut(s) 516, 669, 1053, 1391, 1791
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 4 cut(s) 241, 451, 1334, 1379
MspR9I CCNGG 4 cut(s) 35, 173, 503, 1230
Mva1269I GAATGC 4 cut(s) 753, 1295, 1364, 1442
MvaI CCWGG 1 cut(s) 1230
MvnI CGCG 7 cut(s) 53, 68, 70, 78, 99, 224, 401
MwoI GCNNNNNNNGC 7 cut(s) 109, 234, 460, 535, 719, 851, 968
NaeI GCCGGC 1 cut(s) 87
NciI CCSGG 3 cut(s) 35, 173, 503
NdeI CATATG 1 cut(s) 637
NdeII GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 7 cut(s) 145, 262, 610, 656, 1009, 1295, 1313
NlaIV GGNNCC 3 cut(s) 343, 771, 1580
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 946, 1280
NruI TCGCGA 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 1658
PcsI WCGNNNNNNNCGW 2 cut(s) 272, 303
PctI GAATGC 4 cut(s) 753, 1295, 1364, 1442
PdiI GCCGGC 1 cut(s) 87
PfeI GAWTC 7 cut(s) 313, 614, 806, 1136, 1157, 1372, 1727
Pfl23II CGTACG 2 cut(s) 90, 294
PflFI GACNNNGTC 1 cut(s) 1001
PinAI ACCGGT 1 cut(s) 201
PkrI GCNGC 5 cut(s) 453, 753, 853, 883, 1336
PleI GAGTC 1 cut(s) 380
PmaCI CACGTG 1 cut(s) 1806
PmlI CACGTG 1 cut(s) 1806
PpsI GAGTC 1 cut(s) 380
Ppu21I YACGTR 3 cut(s) 298, 1125, 1806
Psp6I CCWGG 1 cut(s) 1228
PspCI CACGTG 1 cut(s) 1806
PspGI CCWGG 1 cut(s) 1228
PspLI CGTACG 2 cut(s) 90, 294
PspN4I GGNNCC 3 cut(s) 343, 771, 1580
PspOMI GGGCCC 1 cut(s) 1578
PspPI GGNCC 5 cut(s) 55, 435, 1193, 1578, 1579
PstI CTGCAG 2 cut(s) 886, 1637
PstNI CAGNNNCTG 1 cut(s) 1730
PsuI RGATCY 1 cut(s) 1567
PsyI GACNNNGTC 1 cut(s) 1001
PvuII CAGCTG 4 cut(s) 241, 451, 1334, 1379
RruI TCGCGA 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 869
Rsr2I CGGWCCG 2 cut(s) 55, 435
RsrII CGGWCCG 2 cut(s) 55, 435
SapI GCTCTTC 1 cut(s) 1658
SaqAI TTAA 5 cut(s) 516, 669, 1053, 1391, 1791
SatI GCNGC 5 cut(s) 452, 752, 852, 882, 1335
Sau3AI GATC 6 cut(s) 219, 231, 895, 1076, 1567, 1594
Sau96I GGNCC 5 cut(s) 55, 435, 1193, 1578, 1579
SchI GAGTC 1 cut(s) 381
ScrFI CCNGG 4 cut(s) 35, 173, 503, 1230
SduI GDGCHC 3 cut(s) 105, 837, 1582
SfaNI GCATC 3 cut(s) 635, 1372, 1510
SfcI CTRYAG 2 cut(s) 882, 1633
SinI GGWCC 3 cut(s) 55, 435, 1193
SmiMI CAYNNNNRTG 1 cut(s) 869
SnaBI TACGTA 1 cut(s) 1125
Sse9I AATT 6 cut(s) 496, 511, 543, 671, 1239, 1451
SsiI CCGC 8 cut(s) 53, 76, 287, 463, 851, 962, 1209, 1316
SspI AATATT 1 cut(s) 1682
SspMI CTAG 3 cut(s) 530, 774, 1197
StyD4I CCNGG 4 cut(s) 33, 171, 501, 1228
StyI CCWWGG 2 cut(s) 345, 917
TaaI ACNGT 5 cut(s) 445, 1001, 1499, 1743, 1787
TaiI ACGT 7 cut(s) 18, 32, 84, 300, 1127, 1491, 1808
TaqI TCGA 2 cut(s) 333, 745
TaqII GACCGA 2 cut(s) 239, 452
TasI AATT 6 cut(s) 496, 511, 543, 671, 1239, 1451
TatI WGTACW 4 cut(s) 243, 255, 1550, 1738
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 7 cut(s) 313, 614, 806, 1136, 1157, 1372, 1727
Tru1I TTAA 5 cut(s) 516, 669, 1053, 1391, 1791
Tru9I TTAA 5 cut(s) 516, 669, 1053, 1391, 1791
TscAI CASTG 2 cut(s) 690, 1006
TseFI GTSAC 2 cut(s) 946, 1280
TseI GCWGC 4 cut(s) 451, 751, 881, 1334
Tsp45I GTSAC 2 cut(s) 946, 1280
TspDTI ATGAA 4 cut(s) 572, 593, 641, 887
TspGWI ACGGA 1 cut(s) 1416
TspRI CASTG 2 cut(s) 690, 1006
Tth111I GACNNNGTC 1 cut(s) 1001
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 3 cut(s) 55, 435, 1193
XapI RAATTY 1 cut(s) 671
XceI RCATGY 1 cut(s) 145
XspI CTAG 3 cut(s) 530, 774, 1197
ZraI GACGTC 1 cut(s) 1489
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.