MD15G1320400.v1.1

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009505
Physical Location & Seq
Forward (+)
33305709 .. 33312035
6327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1320400.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGTCCGAAAACGACGTCGTGACGCGGACGTTCCGGGCCCTGGTGGAGGGCGCCGACCGGAAGTTTGCTAGGGTTCGCGACGTGCCGGCTTACGGACGCCTCCACAACCAGCACTACTTCCACAAGGTCTTCAAGGCCTACATGCGCCTGTGGAAGTACCAGCAGGAGAACCGCTCTAAGCTCGTCGCCGACGGCCTCAACCGCTGGGAAATCGGCGAGATCGCCAGCCGGATCGGCCAGCTCTACTTCGGCCAGTACATGCGCACCAGCGAGGCTCGATTCCTCCTCGAGGCATACGTCTTCTACGAGGCCATTCTCAGCCGCAGCTACTTCGACGGCTCCACCGGTTCCAGAGGTTTCGGGAAGAAGGATATCGGAGTCCGCTTCAAGGAGTTGAGATTCTACGCCCGGTTTTTGCTCGTTTCCTTGATTTTGAACCGGACCGAGACGGTTCATCTTCTCGCCGAGCGGTTCAAATCGCTTGTTGATGACTGCAAAGCCAATTTTCGGGACACTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGCTCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCATTGCGATACTCTACCTTGTTTGATTCACATCCAGCATCTCTTCCATATGTTGCTCGTTTCCATGCAAAGAGAGTCTTAAAATTTCAAGATGCATTGTTAACAAGCTATCACCATAATGAGGTCAAATTTGCTGAACTTACAATGGACACCTACCGAATGCTGCAATGTTTGGAATGGGAGCCAAGTGGGTCTTTCTACCAAAAGCGTCCAGTTGAACCAAAGGAGAATGGTGTTTTTATTGAACACTCTGGGGCTTCTGCGGCATCTGGAGTAATTGATATGAACTTGGCTGCGGACATGACTGATCCAAGTTTACCGCCAAATCCAAGGAAAGCTGTTATCTATCGGCCATCTGTGACACATTTGATAGCGGTGATGGCTACAATATGTGAGGAGCTTCCTCTGGACAGTATATTGCTAGTTTATCTGTCAGCCTCAGGGAAGGTTGGTCGCAGTAATGCTAGTCAAGTGTACAGCTCTGGAGGATCACAGAAGTCCTCAAAAAACAAAGCTCCTCTCCAAGATCAGAATAAGTTTCTGCCTGAATCATGTATAAATAACAAGGGAGAGTCAAGTGGCTATTATGATCAATATCTGTGGTTTGGTCCTAGAGGAAATGGTGGTTTGAATAACCTTTACCCTGGCGATATAATTCCTTTTACTCGTAGACCTCTTTTCTTGGTAATAGATAGCGACAACAGCCATGCATTCAAGGTTTTACATGGAGCAGAAAGAGGAGAGACGGCTGCTCTGCTTCTTTCACCTTTGAGACCAGCATTCAAGAATCCAGCTGATGCTGATTTAACGCAAAATGGAAGTCAGTTTACCTTTTTCTTGACTGCTCCCTTGGCAGCATTCTGCCAATTAGTTGGATTCTCCTCTTCTGATACCGAAGCAGAGGTTTATAGCAATGCTGAAAGCATACTCTCGGCTGCCTTCTCTGAGTGGGAAGTTATTCTTTGTACAACAAGAAGCCTCGATCTGGTATGGGCACAAGTTCTATCAGATCCTTTTTTGCGGCGACTTATTCTGAGATTTATATTCTGCAGAGCTGTGCTTTCTTTCTTCTGCCCTCCTGAAGATAGGGAGCAGCATTTGCCGGTTTGCCTACCTCTTCTTCCCAATCCCGTTTCTCCAGATTCTGAAGTTGTGCGGTCTGCTGTCCACAGGCTTGCAAAGCACCTCAGCGTCTCTGACTATTTTCACTTTGTTGACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

606

Amino Acids

68.78

Weight (kDa)

8.66

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 14 - 556 2.4e-201 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AbsI CCTCGAGG 1 cut(s) 287
Acc16I TGCGCA 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 50
AccBSI CCGCTC 2 cut(s) 174, 469
AccI GTMKAC 1 cut(s) 1264
AccII CGCG 2 cut(s) 25, 78
AclWI GGATC 4 cut(s) 239, 896, 1090, 1598
AcoI YGGCCR 3 cut(s) 235, 250, 944
AcsI RAATTY 2 cut(s) 677, 722
AcuI CTGAAG 2 cut(s) 1695, 1761
AcyI GRCGYC 3 cut(s) 15, 51, 97
AfaI GTAC 4 cut(s) 158, 257, 1070, 1561
AfiI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
AgeI ACCGGT 1 cut(s) 344
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 2 cut(s) 39, 1237
Alw26I GTCTC 4 cut(s) 440, 1331, 1360, 1792
AlwI GGATC 4 cut(s) 239, 896, 1090, 1598
AlwNI CAGNNNCTG 1 cut(s) 1739
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 9 cut(s) 36, 135, 193, 235, 250, 309, 576, 593, 944
ApaI GGGCCC 1 cut(s) 40
ApeKI GCWGC 7 cut(s) 324, 757, 887, 1343, 1448, 1529, 1687
ApoI RAATTY 2 cut(s) 677, 722
ArsI GACNNNNNNTTYG 5 cut(s) 32, 47, 79, 1399, 1431
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 1551
AspLEI GCGC 3 cut(s) 53, 147, 264
AspS9I GGNCC 4 cut(s) 36, 37, 441, 1202
AsuC2I CCSGG 2 cut(s) 35, 409
AsuHPI GGTGA 3 cut(s) 698, 982, 1350
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 2 cut(s) 441, 1202
AxyI CCTNAGG 1 cut(s) 1033
BaeGI GKGCMC 2 cut(s) 40, 1591
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 40
BarI GAAGNNNNNNTAC 2 cut(s) 1405, 1437
BbsI GAAGAC 2 cut(s) 121, 292
BbvCI CCTCAGC 1 cut(s) 1781
BbvI GCAGC 7 cut(s) 336, 744, 874, 1330, 1460, 1516, 1699
BccI CCATC 2 cut(s) 955, 967
BceAI ACGGC 4 cut(s) 208, 352, 591, 1356
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 41, 1239
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 2 cut(s) 35, 409
BcoDI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BfaI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
BfmI CTRYAG 1 cut(s) 1642
BfoI RGCGCY 1 cut(s) 54
BglI GCCNNNNNGGC 1 cut(s) 234
Bme1390I CCNGG 4 cut(s) 35, 41, 409, 1239
Bme18I GGWCC 2 cut(s) 441, 1202
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 36, 37, 441, 1202
BmiI GGNNCC 5 cut(s) 38, 52, 340, 349, 777
BmrFI CCNGG 4 cut(s) 35, 41, 409, 1239
BmsI GCATC 4 cut(s) 641, 676, 869, 1381
BpiI GAAGAC 2 cut(s) 121, 292
BplI GAGNNNNNCTC 2 cut(s) 158, 190
BpmI CTGGAG 3 cut(s) 885, 1098, 1716
Bpu10I CCTNAGC 1 cut(s) 1781
BpuMI CCSGG 2 cut(s) 35, 409
BsaBI GATNNNNATC 2 cut(s) 624, 1188
BsaHI GRCGYC 3 cut(s) 15, 51, 97
BsaI GGTCTC 1 cut(s) 1360
BsaJI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BsaWI WCCGGW 3 cut(s) 57, 344, 438
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
Bse118I RCCGGY 3 cut(s) 85, 344, 1696
Bse1I ACTGG 2 cut(s) 253, 806
Bse21I CCTNAGG 1 cut(s) 1033
Bse3DI GCAATG 3 cut(s) 596, 767, 1513
Bse8I GATNNNNATC 2 cut(s) 624, 1188
BseBI CCWGG 2 cut(s) 41, 1239
BseDI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 2 cut(s) 624, 1188
BseLI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
BseMI GCAATG 3 cut(s) 596, 767, 1513
BseMII CTCAG 5 cut(s) 331, 1047, 1530, 1619, 1795
BseNI ACTGG 2 cut(s) 253, 806
BseRI GAGGAG 5 cut(s) 275, 1004, 1101, 1347, 1465
BseSI GKGCMC 2 cut(s) 40, 1591
BseXI GCAGC 7 cut(s) 336, 744, 874, 1330, 1460, 1516, 1699
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 25, 78
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 9 cut(s) 38, 137, 195, 237, 252, 311, 578, 595, 946
BshNI GGYRCC 1 cut(s) 50
BshTI ACCGGT 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 8 cut(s) 34, 58, 86, 229, 345, 409, 439, 1697
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 8 cut(s) 40, 46, 92, 289, 388, 507, 715, 1579
BsmAI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BsmBI CGTCTC 3 cut(s) 440, 1331, 1792
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 4 cut(s) 759, 1304, 1373, 1451
BsnI GGCC 9 cut(s) 38, 137, 195, 237, 252, 311, 578, 595, 946
Bso31I GGTCTC 1 cut(s) 1360
BsoBI CYCGRG 1 cut(s) 287
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 2 cut(s) 40, 1591
Bsp1407I TGTACA 2 cut(s) 1068, 1559
Bsp143I GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
Bsp68I TCGCGA 1 cut(s) 78
BspANI GGCC 9 cut(s) 38, 137, 195, 237, 252, 311, 578, 595, 946
BspCNI CTCAG 5 cut(s) 330, 1046, 1531, 1620, 1794
BspFNI CGCG 2 cut(s) 25, 78
BspLI GGNNCC 5 cut(s) 38, 52, 340, 349, 777
BspMAI CTGCAG 1 cut(s) 1646
BspPI GGATC 4 cut(s) 239, 896, 1090, 1598
BspT107I GGYRCC 1 cut(s) 50
BspTNI GGTCTC 1 cut(s) 1360
BsrBI CCGCTC 2 cut(s) 174, 469
BsrDI GCAATG 3 cut(s) 596, 767, 1513
BsrFI RCCGGY 3 cut(s) 85, 344, 1696
BsrGI TGTACA 2 cut(s) 1068, 1559
BsrI ACTGG 2 cut(s) 253, 806
BssAI RCCGGY 3 cut(s) 85, 344, 1696
BssECI CCNNGG 4 cut(s) 39, 923, 1237, 1443
BssMI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
BssNI GRCGYC 3 cut(s) 15, 51, 97
BssT1I CCWWGG 2 cut(s) 923, 1443
Bst2UI CCWGG 2 cut(s) 41, 1239
Bst4CI ACNGT 2 cut(s) 451, 1007
Bst6I CTCTTC 3 cut(s) 642, 1483, 1716
BstACI GRCGYC 3 cut(s) 15, 51, 97
BstAPI GCANNNNNTGC 1 cut(s) 1693
BstAUI TGTACA 2 cut(s) 1068, 1559
BstC8I GCNNGC 4 cut(s) 87, 226, 239, 1770
BstDEI CTNAG 6 cut(s) 177, 317, 1033, 1539, 1628, 1781
BstENI CCTNNNNNAGG 2 cut(s) 44, 287
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 2 cut(s) 25, 78
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 147, 264
BstKTI GATC 8 cut(s) 222, 234, 904, 1085, 1123, 1186, 1579, 1606
BstMAI GTCTC 4 cut(s) 440, 1331, 1360, 1792
BstMBI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
BstNI CCWGG 2 cut(s) 41, 1239
BstNSI RCATGY 2 cut(s) 145, 262
BstSCI CCNGG 4 cut(s) 33, 39, 407, 1237
BstSFI CTRYAG 1 cut(s) 1642
BstSLI GKGCMC 2 cut(s) 40, 1591
BstUI CGCG 2 cut(s) 25, 78
BstV1I GCAGC 7 cut(s) 336, 744, 874, 1330, 1460, 1516, 1699
BstV2I GAAGAC 2 cut(s) 121, 292
BstX2I RGATCY 1 cut(s) 1603
BstXI CCANNNNNNTGG 1 cut(s) 1466
BstYI RGATCY 1 cut(s) 1603
Bsu36I CCTNAGG 1 cut(s) 1033
BsuRI GGCC 9 cut(s) 38, 137, 195, 237, 252, 311, 578, 595, 946
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 625
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 4 cut(s) 87, 226, 239, 1770
CaiI CAGNNNCTG 1 cut(s) 1739
CfoI GCGC 3 cut(s) 53, 147, 264
Cfr10I RCCGGY 3 cut(s) 85, 344, 1696
Cfr13I GGNCC 4 cut(s) 36, 37, 441, 1202
CpoI CGGWCCG 1 cut(s) 441
CseI GACGC 4 cut(s) 31, 105, 791, 1774
Csp6I GTAC 4 cut(s) 157, 256, 1069, 1560
CspAI ACCGGT 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 1175, 1210
CspI CGGWCCG 1 cut(s) 441
CviAII CATG 8 cut(s) 142, 259, 659, 895, 1146, 1301, 1319, 1815
CviQI GTAC 4 cut(s) 157, 256, 1069, 1560
DdeI CTNAG 6 cut(s) 177, 317, 1033, 1539, 1628, 1781
DinI GGCGCC 1 cut(s) 52
DpnI GATC 8 cut(s) 221, 233, 903, 1084, 1122, 1185, 1578, 1605
DpnII GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 3 cut(s) 235, 250, 944
Eam1104I CTCTTC 3 cut(s) 642, 1483, 1716
EarI CTCTTC 3 cut(s) 642, 1483, 1716
Eco130I CCWWGG 2 cut(s) 923, 1443
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 1360
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 441, 1202
Eco57I CTGAAG 2 cut(s) 1695, 1761
Eco81I CCTNAGG 1 cut(s) 1033
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 2 cut(s) 44, 287
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 39, 1237
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 923, 1443
EcoT22I ATGCAT 2 cut(s) 691, 1306
EcoT38I GRGCYC 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 923, 1443
Esp3I CGTCTC 3 cut(s) 440, 1331, 1792
FaeI CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
FalI AAGNNNNNCTT 6 cut(s) 656, 688, 772, 804, 1539, 1571
FaqI GGGAC 1 cut(s) 524
FatI CATG 8 cut(s) 141, 258, 658, 894, 1145, 1300, 1318, 1814
FauNDI CATATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 1183
FblI GTMKAC 1 cut(s) 1264
FokI GGATG 1 cut(s) 612
FriOI GRGCYC 1 cut(s) 40
FspAI RTGCGCAY 1 cut(s) 263
FspBI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 3 cut(s) 52, 146, 263
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 3 cut(s) 885, 1098, 1716
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 9 cut(s) 38, 137, 195, 237, 252, 311, 578, 595, 946
HapII CCGG 8 cut(s) 34, 58, 86, 229, 345, 409, 439, 1697
HgaI GACGC 4 cut(s) 31, 105, 791, 1774
HhaI GCGC 3 cut(s) 53, 147, 264
Hin1I GRCGYC 3 cut(s) 15, 51, 97
Hin1II CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
Hin6I GCGC 3 cut(s) 51, 145, 262
HinP1I GCGC 3 cut(s) 51, 145, 262
HincII GTYRAC 2 cut(s) 696, 1810
HindII GTYRAC 2 cut(s) 696, 1810
HpaI GTTAAC 1 cut(s) 696
HpaII CCGG 8 cut(s) 34, 58, 86, 229, 345, 409, 439, 1697
HphI GGTGA 3 cut(s) 698, 982, 1350
Hpy166II GTNNAC 7 cut(s) 696, 911, 1069, 1265, 1422, 1762, 1810
Hpy188I TCNGA 9 cut(s) 7, 377, 1125, 1483, 1540, 1603, 1629, 1741, 1792
Hpy8I GTNNAC 7 cut(s) 696, 911, 1069, 1265, 1422, 1762, 1810
Hpy99I CGWCG 6 cut(s) 17, 20, 83, 188, 194, 338
HpyAV CCTTC 3 cut(s) 361, 1033, 1543
HpyCH4III ACNGT 2 cut(s) 451, 1007
HpyCH4IV ACGT 4 cut(s) 15, 29, 81, 297
HpyCH4V TGCA 8 cut(s) 495, 544, 662, 689, 760, 1304, 1644, 1772
HpyF10VI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
HpyF3I CTNAG 6 cut(s) 177, 317, 1033, 1539, 1628, 1781
HpySE526I ACGT 4 cut(s) 15, 29, 81, 297
Hsp92I GRCGYC 3 cut(s) 15, 51, 97
Hsp92II CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
HspAI GCGC 3 cut(s) 51, 145, 262
KasI GGCGCC 1 cut(s) 50
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 1183
KspAI GTTAAC 1 cut(s) 696
Kzo9I GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
LmnI GCTCC 7 cut(s) 344, 775, 991, 1114, 1322, 1444, 1684
Lsp1109I GCAGC 7 cut(s) 336, 744, 874, 1330, 1460, 1516, 1699
LweI GCATC 4 cut(s) 641, 676, 869, 1381
MaeI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
MaeII ACGT 4 cut(s) 15, 29, 81, 297
MaeIII GTNAC 2 cut(s) 19, 952
MalI GATC 8 cut(s) 221, 233, 903, 1084, 1122, 1185, 1578, 1605
MbiI CCGCTC 2 cut(s) 174, 469
MboI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
MflI RGATCY 1 cut(s) 1603
MhlI GDGCHC 2 cut(s) 40, 1591
MluCI AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 3 cut(s) 387, 678, 1175
MmeI TCCRAC 1 cut(s) 1447
Mph1103I ATGCAT 2 cut(s) 691, 1306
MroNI GCCGGC 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 1551
MseI TTAA 4 cut(s) 522, 674, 695, 1400
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 2 cut(s) 204, 1388
MspI CCGG 8 cut(s) 34, 58, 86, 229, 345, 409, 439, 1697
MspR9I CCNGG 4 cut(s) 35, 41, 409, 1239
Mva1269I GAATGC 4 cut(s) 759, 1304, 1373, 1451
MvaI CCWGG 2 cut(s) 41, 1239
MvnI CGCG 2 cut(s) 25, 78
MwoI GCNNNNNNNGC 9 cut(s) 201, 234, 541, 857, 974, 1296, 1445, 1693, 1774
NaeI GCCGGC 1 cut(s) 87
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 2 cut(s) 35, 409
NdeI CATATG 1 cut(s) 643
NdeII GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 8 cut(s) 145, 262, 662, 898, 1149, 1304, 1322, 1818
NlaIV GGNNCC 5 cut(s) 38, 52, 340, 349, 777
NmeAIII GCCGAG 2 cut(s) 490, 1505
NmuCI GTSAC 2 cut(s) 19, 952
NruI TCGCGA 1 cut(s) 78
NsbI TGCGCA 1 cut(s) 263
NsiI ATGCAT 2 cut(s) 691, 1306
NspI RCATGY 2 cut(s) 145, 262
PaeR7I CTCGAG 1 cut(s) 287
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 303
PctI GAATGC 4 cut(s) 759, 1304, 1373, 1451
PdiI GCCGGC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 1551
PfeI GAWTC 7 cut(s) 279, 399, 620, 1142, 1381, 1470, 1736
PinAI ACCGGT 1 cut(s) 344
PleI GAGTC 3 cut(s) 386, 677, 1174
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 3 cut(s) 386, 677, 1174
Psp6I CCWGG 2 cut(s) 39, 1237
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 2 cut(s) 39, 1237
PspN4I GGNNCC 5 cut(s) 38, 52, 340, 349, 777
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 4 cut(s) 36, 37, 441, 1202
PspXI VCTCGAGB 1 cut(s) 287
PstI CTGCAG 1 cut(s) 1646
PstNI CAGNNNCTG 1 cut(s) 1739
PsuI RGATCY 1 cut(s) 1603
PvuII CAGCTG 1 cut(s) 1388
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 4 cut(s) 158, 257, 1070, 1561
RsaNI GTAC 4 cut(s) 157, 256, 1069, 1560
RseI CAYNNNNRTG 1 cut(s) 711
Rsr2I CGGWCCG 1 cut(s) 441
RsrII CGGWCCG 1 cut(s) 441
SaqAI TTAA 4 cut(s) 522, 674, 695, 1400
Sau3AI GATC 8 cut(s) 219, 231, 901, 1082, 1120, 1183, 1576, 1603
Sau96I GGNCC 4 cut(s) 36, 37, 441, 1202
SchI GAGTC 3 cut(s) 387, 678, 1175
ScrFI CCNGG 4 cut(s) 35, 41, 409, 1239
SduI GDGCHC 2 cut(s) 40, 1591
SfaNI GCATC 4 cut(s) 641, 676, 869, 1381
SfcI CTRYAG 1 cut(s) 1642
SfoI GGCGCC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 2 cut(s) 441, 1202
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
SseBI AGGCCT 1 cut(s) 137
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 4 cut(s) 33, 39, 407, 1237
StyI CCWWGG 2 cut(s) 923, 1443
TaaI ACNGT 2 cut(s) 451, 1007
TaiI ACGT 4 cut(s) 18, 32, 84, 300
TaqI TCGA 5 cut(s) 277, 288, 333, 556, 1575
TaqII GACCGA 1 cut(s) 458
TasI AATT 8 cut(s) 502, 517, 549, 677, 722, 870, 1248, 1460
TatI WGTACW 3 cut(s) 255, 1068, 1559
TauI GCSGC 3 cut(s) 324, 860, 1618
TfiI GAWTC 7 cut(s) 279, 399, 620, 1142, 1381, 1470, 1736
Tru1I TTAA 4 cut(s) 522, 674, 695, 1400
Tru9I TTAA 4 cut(s) 522, 674, 695, 1400
TseFI GTSAC 2 cut(s) 19, 952
TseI GCWGC 7 cut(s) 324, 757, 887, 1343, 1448, 1529, 1687
Tsp45I GTSAC 2 cut(s) 19, 952
TspDTI ATGAA 4 cut(s) 443, 578, 599, 893
TspGWI ACGGA 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 441, 1202
XagI CCTNNNNNAGG 2 cut(s) 44, 287
XapI RAATTY 2 cut(s) 677, 722
XceI RCATGY 2 cut(s) 145, 262
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1551
XspI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
ZraI GACGTC 1 cut(s) 16
Zsp2I ATGCAT 2 cut(s) 691, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.