RLG00000017975

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009505
Physical Location & Seq
Forward (+)
23963575 .. 23968301
4727 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017975

Sequence Viewer

Length: 1809 bp
ATGTCAGATAACGACGTTGTTTCCGGCACGTTCCGGGCGCTGGTGGAGAGCGCGGACCGGAAGTTCGCGAGGGTCCGGGACGTGCCGGCGTACGGGCGCGTGCACAACCAGCACTACTTTCACAAGGTGTTCAAGGCGTACATGCGGCTGTGGAAGTACCAGCAGGAGAACCGGGCCAAGCTAGTCGAGGCCGGTCTGAACCGGTGGGAAATCGGCGAGATCGCCAGCCGGATCGGCCAGCTGTACTTCGGGCAGTACATGAGGACGAGCGAGGCGAGGTTTCTGGCCGAGGCGTACGTCTTCTACGAGGCCATACTGAGCCGGCGGTACTTCGAGGGAGGGTCCAAGAGTTTGGGGAAGAAGGATCTTGGAGTCCGGTTCAAGGAGTTGAGATTCTACGCTAGGTTCTTGCTGGTTTCGTTGATTTTGAACCGGACCGAGATGGTTCAGCTGCTCGCCGAGCGGTTCAAGGCTCTGGTTGATGACAGCAGAGCCAATTACCGGGAAACTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGTGCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCGCTGCGATACTCTGCCATGTTTGATTCACATCCAGCGTCTCTCCCATGTGTGGCTCGTTTCCATGCAAAGAGAGTTCTAAAATTTCAAGATGCACTGCTAACAAGCTATCACCGGAATGAGGCCAAATTTGCTGAACTTACATTGGACTCATACCGAATGCTGCAATGTTTGGAATGGGAGCCAAGCGGATCTTTCTACCAAAAGCGCCCTGTTGAATCAAAAGAGAACGGCTCTTTTATTGAGCACTCTGGGGCTTCTGCGGCTTCTGGAGTCATTGATATGAACTTGGCTGCAGATATGACTGATCCAAGTTTACCTCCAAATCCAAGGAAAGCGGTTATCTATCGTCCATCTGTGACACATTTGATAGCGGTTATGGCAACAATATGTGAAGAACTCCCTGTGGACAGTGTTATGCTACTGTACCTGTCAGCCTCAGGGACAGCTGGTCGTAGTAATGTAAATCAGCTATACCACTCTGGAGGATCACAAAAATCGTCAAAAAATAAAGTTCCTCTCCCAGATCAGAATACTTCTCGGCCTGAATCTTGTAATGGCAAGGCAGAGTCAAGTGGCTATTATGATAAGTATCTGTGGTTTGGTCCTAGAGGAAATGGCGGTGAGAATAACCTTTACCCTGGAGACATAATTCCTTTTACTCGCAGACCTCTTTTCTTGATAGTTGATAGTGACAACAGCCATGCATTCAAGGTTTTACATGGTGCGGAAAGGGGAGAGACAGCTGCTCTGCTTCTTTCACCTTTGAGACCAGCATTCAAGAATCCAGGTGATGCTGATTTAACACAAAACGGAAGCCAGTTCACCTTTTTCTTGACCACTCCTCTCTCAGCATTCTGCCAATTAGTTGGTCTCTCCTCTTCTGACACTGAAACAGACGTTTATAAGGGTGCTGAGAGCATACTTTCTGATGCCTTCTCTAAGTGGGAAGTAATACTGTGTACATCAACAGACATGAATCTGGTATGGGCACAAGTTATACCTGATCCCTTTCTGCGGCGACTTATTATGAGATTTATATTCTGCAGAGCTGTGCTTGTGCTCTTCTGTCCTCCAGAAGATAGTGAACAATATTTGCCGGTTTGTCTACCCTTTCTTCCCAATTCTGTCTCTCCAGATTCTGAAGTAGTGCAATCTACTGTCCTCCGGCTTGCAAAGCACCTCAGAGTTGATGACTGTTTTAACTTTGACAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

603

Amino Acids

68.32

Weight (kDa)

8.51

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 13 - 553 9.1e-201 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1494
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 1696
AccII CGCG 3 cut(s) 53, 68, 99
AclWI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1589
AcoI YGGCCR 2 cut(s) 235, 285
AcsI RAATTY 2 cut(s) 671, 716
AcuI CTGAAG 1 cut(s) 1752
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 9 cut(s) 92, 140, 158, 245, 257, 296, 329, 1016, 1552
AfiI CCNNNNNNNGG 7 cut(s) 40, 92, 382, 501, 640, 709, 1605
AgeI ACCGGT 1 cut(s) 201
AgsI TTSAA 8 cut(s) 133, 382, 430, 469, 677, 806, 1300, 1369
AhdI GACNNNNNGTC 1 cut(s) 1038
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 2 cut(s) 1228, 1375
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 8 cut(s) 181, 241, 451, 696, 1037, 1060, 1334, 1640
AluI AGCT 8 cut(s) 181, 241, 451, 696, 1037, 1060, 1334, 1640
Alw21I GWGCWC 3 cut(s) 105, 837, 1653
Alw26I GTCTC 6 cut(s) 633, 1227, 1322, 1351, 1466, 1723
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1589
AlwNI CAGNNNCTG 1 cut(s) 1730
AoxI GGCC 9 cut(s) 174, 189, 235, 285, 309, 570, 587, 711, 1130
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 5 cut(s) 451, 592, 751, 881, 1334
ApoI RAATTY 2 cut(s) 671, 716
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AsiGI ACCGGT 1 cut(s) 201
AspLEI GCGC 4 cut(s) 40, 53, 99, 798
AspS9I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
AsuC2I CCSGG 4 cut(s) 35, 77, 173, 503
AsuHPI GGTGA 5 cut(s) 692, 1223, 1341, 1391, 1405
AvaII GGWCC 5 cut(s) 55, 73, 342, 435, 1193
AxyI CCTNAGG 1 cut(s) 1027
BaeGI GKGCMC 2 cut(s) 105, 1582
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 3 cut(s) 105, 837, 1653
BbvI GCAGC 5 cut(s) 438, 579, 738, 868, 1321
BccI CCATC 2 cut(s) 436, 949
BceAI ACGGC 2 cut(s) 585, 835
BciT130I CCWGG 2 cut(s) 1230, 1377
BcnI CCSGG 4 cut(s) 35, 77, 173, 503
BcoDI GTCTC 6 cut(s) 633, 1227, 1322, 1351, 1466, 1723
BfaI CTAG 4 cut(s) 182, 402, 530, 1197
BfmI CTRYAG 2 cut(s) 882, 1633
BfoI RGCGCY 2 cut(s) 41, 799
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 9 cut(s) 146, 452, 590, 593, 752, 852, 882, 1335, 1607
BlsI GCNGC 9 cut(s) 147, 453, 591, 594, 753, 853, 883, 1336, 1608
Bme1390I CCNGG 6 cut(s) 35, 77, 173, 503, 1230, 1377
Bme18I GGWCC 5 cut(s) 55, 73, 342, 435, 1193
BmeRI GACNNNNNGTC 1 cut(s) 1038
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
BmiI GGNNCC 3 cut(s) 74, 343, 771
BmrFI CCNGG 6 cut(s) 35, 77, 173, 503, 1230, 1377
BmsI GCATC 3 cut(s) 670, 1372, 1510
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpmI CTGGAG 5 cut(s) 879, 1092, 1251, 1647, 1707
BpuMI CCSGG 4 cut(s) 35, 77, 173, 503
BsaBI GATNNNNATC 2 cut(s) 618, 1179
BsaI GGTCTC 2 cut(s) 1351, 1466
BsaJI CCNNGG 3 cut(s) 288, 917, 1228
BsaWI WCCGGW 5 cut(s) 57, 201, 375, 432, 702
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 92, 382, 501, 640, 709, 1605
Bse118I RCCGGY 5 cut(s) 85, 191, 201, 321, 1687
Bse1I ACTGG 1 cut(s) 1408
Bse21I CCTNAGG 1 cut(s) 1027
Bse3DI GCAATG 1 cut(s) 761
Bse8I GATNNNNATC 2 cut(s) 618, 1179
BseBI CCWGG 2 cut(s) 1230, 1377
BseDI CCNNGG 3 cut(s) 288, 917, 1228
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 2 cut(s) 618, 1179
BseLI CCNNNNNNNGG 7 cut(s) 40, 92, 382, 501, 640, 709, 1605
BseMI GCAATG 1 cut(s) 761
BseMII CTCAG 5 cut(s) 308, 1041, 1452, 1494, 1786
BseNI ACTGG 1 cut(s) 1408
BseRI GAGGAG 2 cut(s) 1422, 1456
BseSI GKGCMC 2 cut(s) 105, 1582
BseXI GCAGC 5 cut(s) 438, 579, 738, 868, 1321
Bsh1236I CGCG 3 cut(s) 53, 68, 99
BshFI GGCC 9 cut(s) 176, 191, 237, 287, 311, 572, 589, 713, 1132
BshTI ACCGGT 1 cut(s) 201
BsiHKAI GWGCWC 3 cut(s) 105, 837, 1653
BsiWI CGTACG 2 cut(s) 90, 294
BslFI GGGAC 2 cut(s) 92, 1045
BslI CCNNNNNNNGG 7 cut(s) 40, 92, 382, 501, 640, 709, 1605
BsmAI GTCTC 6 cut(s) 633, 1227, 1322, 1351, 1466, 1723
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 2 cut(s) 92, 1045
BsmI GAATGC 4 cut(s) 753, 1295, 1364, 1442
BsnI GGCC 9 cut(s) 176, 191, 237, 287, 311, 572, 589, 713, 1132
Bso31I GGTCTC 2 cut(s) 1351, 1466
Bsp1286I GDGCHC 4 cut(s) 105, 837, 1582, 1653
Bsp1407I TGTACA 1 cut(s) 1550
Bsp143I GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
Bsp68I TCGCGA 1 cut(s) 68
BspANI GGCC 9 cut(s) 176, 191, 237, 287, 311, 572, 589, 713, 1132
BspCNI CTCAG 5 cut(s) 309, 1040, 1451, 1495, 1785
BspFNI CGCG 3 cut(s) 53, 68, 99
BspLI GGNNCC 3 cut(s) 74, 343, 771
BspMAI CTGCAG 2 cut(s) 886, 1637
BspPI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1589
BspQI GCTCTTC 1 cut(s) 1658
BspTNI GGTCTC 2 cut(s) 1351, 1466
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 761
BsrFI RCCGGY 5 cut(s) 85, 191, 201, 321, 1687
BsrGI TGTACA 1 cut(s) 1550
BsrI ACTGG 1 cut(s) 1408
BssAI RCCGGY 5 cut(s) 85, 191, 201, 321, 1687
BssECI CCNNGG 3 cut(s) 288, 917, 1228
BssMI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
BssT1I CCWWGG 1 cut(s) 917
Bst2UI CCWGG 2 cut(s) 1230, 1377
Bst4CI ACNGT 5 cut(s) 1001, 1014, 1548, 1750, 1787
Bst6I CTCTTC 2 cut(s) 1474, 1658
BstAUI TGTACA 1 cut(s) 1550
BstC8I GCNNGC 7 cut(s) 87, 101, 226, 239, 323, 456, 1761
BstDEI CTNAG 6 cut(s) 317, 1027, 1438, 1503, 1530, 1772
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 3 cut(s) 53, 68, 99
BstH2I RGCGCY 2 cut(s) 41, 799
BstHHI GCGC 4 cut(s) 40, 53, 99, 798
BstKTI GATC 8 cut(s) 222, 234, 367, 782, 898, 1079, 1117, 1597
BstMAI GTCTC 6 cut(s) 633, 1227, 1322, 1351, 1466, 1723
BstMBI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
BstMWI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1765
BstNI CCWGG 2 cut(s) 1230, 1377
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 6 cut(s) 33, 75, 171, 501, 1228, 1375
BstSFI CTRYAG 2 cut(s) 882, 1633
BstSLI GKGCMC 2 cut(s) 105, 1582
BstUI CGCG 3 cut(s) 53, 68, 99
BstV1I GCAGC 5 cut(s) 438, 579, 738, 868, 1321
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 2 cut(s) 364, 779
BstXI CCANNNNNNTGG 2 cut(s) 352, 1457
BstYI RGATCY 2 cut(s) 364, 779
Bsu36I CCTNAGG 1 cut(s) 1027
BsuRI GGCC 9 cut(s) 176, 191, 237, 287, 311, 572, 589, 713, 1132
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 683
BtsIMutI CAGTG 3 cut(s) 683, 1006, 1476
BtuMI TCGCGA 1 cut(s) 68
Cac8I GCNNGC 7 cut(s) 87, 101, 226, 239, 323, 456, 1761
CaiI CAGNNNCTG 1 cut(s) 1730
CfoI GCGC 4 cut(s) 40, 53, 99, 798
Cfr10I RCCGGY 5 cut(s) 85, 191, 201, 321, 1687
Cfr13I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
CpoI CGGWCCG 2 cut(s) 55, 435
CseI GACGC 1 cut(s) 615
Csp6I GTAC 9 cut(s) 91, 139, 157, 244, 256, 295, 328, 1015, 1551
CspAI ACCGGT 1 cut(s) 201
CspI CGGWCCG 2 cut(s) 55, 435
CviAII CATG 9 cut(s) 142, 259, 607, 636, 653, 1292, 1310, 1564, 1806
CviQI GTAC 9 cut(s) 91, 139, 157, 244, 256, 295, 328, 1015, 1551
DdeI CTNAG 6 cut(s) 317, 1027, 1438, 1503, 1530, 1772
DpnI GATC 8 cut(s) 221, 233, 366, 781, 897, 1078, 1116, 1596
DpnII GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
DraI TTTAAA 1 cut(s) 517
DraIII CACNNNGTG 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 1038
EaeI YGGCCR 2 cut(s) 235, 285
Eam1104I CTCTTC 2 cut(s) 1474, 1658
Eam1105I GACNNNNNGTC 1 cut(s) 1038
EarI CTCTTC 2 cut(s) 1474, 1658
Eco130I CCWWGG 1 cut(s) 917
Eco31I GGTCTC 2 cut(s) 1351, 1466
Eco47I GGWCC 5 cut(s) 55, 73, 342, 435, 1193
Eco57I CTGAAG 1 cut(s) 1752
Eco81I CCTNAGG 1 cut(s) 1027
EcoRII CCWGG 2 cut(s) 1228, 1375
EcoT14I CCWWGG 1 cut(s) 917
EcoT22I ATGCAT 1 cut(s) 1297
ErhI CCWWGG 1 cut(s) 917
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 9 cut(s) 145, 262, 610, 639, 656, 1295, 1313, 1567, 1809
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 2 cut(s) 92, 1045
FatI CATG 9 cut(s) 141, 258, 606, 635, 652, 1291, 1309, 1563, 1805
FblI GTMKAC 1 cut(s) 1696
Fnu4HI GCNGC 9 cut(s) 146, 452, 590, 593, 752, 852, 882, 1335, 1607
FokI GGATG 1 cut(s) 606
Fsp4HI GCNGC 9 cut(s) 146, 452, 590, 593, 752, 852, 882, 1335, 1607
FspBI CTAG 4 cut(s) 182, 402, 530, 1197
GlaI GCGC 4 cut(s) 39, 52, 98, 797
GluI GCNGC 9 cut(s) 146, 452, 590, 593, 752, 852, 882, 1335, 1607
GsuI CTGGAG 5 cut(s) 879, 1092, 1251, 1647, 1707
HaeII RGCGCY 2 cut(s) 41, 799
HaeIII GGCC 9 cut(s) 176, 191, 237, 287, 311, 572, 589, 713, 1132
HgaI GACGC 1 cut(s) 615
HhaI GCGC 4 cut(s) 40, 53, 99, 798
Hin1II CATG 9 cut(s) 145, 262, 610, 639, 656, 1295, 1313, 1567, 1809
Hin6I GCGC 4 cut(s) 38, 51, 97, 796
HinP1I GCGC 4 cut(s) 38, 51, 97, 796
HphI GGTGA 5 cut(s) 692, 1223, 1341, 1391, 1405
Hpy166II GTNNAC 7 cut(s) 103, 905, 997, 1413, 1551, 1676, 1697
Hpy188I TCNGA 7 cut(s) 7, 198, 1119, 1474, 1519, 1732, 1775
Hpy188III TCNNGA 9 cut(s) 67, 677, 858, 1071, 1267, 1369, 1423, 1664, 1724
Hpy8I GTNNAC 7 cut(s) 103, 905, 997, 1413, 1551, 1676, 1697
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 355, 1534
HpyCH4III ACNGT 5 cut(s) 1001, 1014, 1548, 1750, 1787
HpyCH4IV ACGT 5 cut(s) 15, 29, 81, 297, 1488
HpyF10VI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1765
HpyF3I CTNAG 6 cut(s) 317, 1027, 1438, 1503, 1530, 1772
HpySE526I ACGT 5 cut(s) 15, 29, 81, 297, 1488
Hsp92II CATG 9 cut(s) 145, 262, 610, 639, 656, 1295, 1313, 1567, 1809
HspAI GCGC 4 cut(s) 38, 51, 97, 796
KroI GCCGGC 2 cut(s) 85, 321
KroNI GCCGGC 2 cut(s) 87, 323
Kzo9I GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
LguI GCTCTTC 1 cut(s) 1658
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 5 cut(s) 438, 579, 738, 868, 1321
LweI GCATC 3 cut(s) 670, 1372, 1510
MaeI CTAG 4 cut(s) 182, 402, 530, 1197
MaeII ACGT 5 cut(s) 15, 29, 81, 297, 1488
MaeIII GTNAC 2 cut(s) 946, 1280
MalI GATC 8 cut(s) 221, 233, 366, 781, 897, 1078, 1116, 1596
MbiI CCGCTC 1 cut(s) 463
MboI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
MboII GAAGA 7 cut(s) 292, 370, 995, 1461, 1645, 1679, 1697
MflI RGATCY 2 cut(s) 364, 779
MhlI GDGCHC 4 cut(s) 105, 837, 1582, 1653
MluCI AATT 8 cut(s) 496, 511, 543, 671, 716, 1239, 1451, 1711
MlyI GAGTC 4 cut(s) 381, 731, 870, 1166
Mph1103I ATGCAT 1 cut(s) 1297
MroNI GCCGGC 2 cut(s) 85, 321
MseI TTAA 3 cut(s) 516, 1391, 1791
MslI CAYNNNNRTG 2 cut(s) 705, 869
MspA1I CMGCKG 5 cut(s) 241, 451, 592, 1037, 1334
MspR9I CCNGG 6 cut(s) 35, 77, 173, 503, 1230, 1377
Mva1269I GAATGC 4 cut(s) 753, 1295, 1364, 1442
MvaI CCWGG 2 cut(s) 1230, 1377
MvnI CGCG 3 cut(s) 53, 68, 99
MwoI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1765
NaeI GCCGGC 2 cut(s) 87, 323
NciI CCSGG 4 cut(s) 35, 77, 173, 503
NdeII GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
NgoMIV GCCGGC 2 cut(s) 85, 321
NlaIII CATG 9 cut(s) 145, 262, 610, 639, 656, 1295, 1313, 1567, 1809
NlaIV GGNNCC 3 cut(s) 74, 343, 771
NmeAIII GCCGAG 3 cut(s) 313, 484, 1108
NmuCI GTSAC 2 cut(s) 946, 1280
NruI TCGCGA 1 cut(s) 68
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 1658
PcsI WCGNNNNNNNCGW 2 cut(s) 272, 303
PctI GAATGC 4 cut(s) 753, 1295, 1364, 1442
PdiI GCCGGC 2 cut(s) 87, 323
PfeI GAWTC 7 cut(s) 393, 614, 806, 1136, 1372, 1567, 1727
Pfl23II CGTACG 2 cut(s) 90, 294
PfoI TCCNGGA 1 cut(s) 75
PinAI ACCGGT 1 cut(s) 201
PkrI GCNGC 9 cut(s) 147, 453, 591, 594, 753, 853, 883, 1336, 1608
PleI GAGTC 4 cut(s) 380, 731, 869, 1165
PpsI GAGTC 4 cut(s) 380, 731, 869, 1165
PsiI TTATAA 1 cut(s) 1494
Psp6I CCWGG 2 cut(s) 1228, 1375
PspGI CCWGG 2 cut(s) 1228, 1375
PspLI CGTACG 2 cut(s) 90, 294
PspN4I GGNNCC 3 cut(s) 74, 343, 771
PspPI GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
PstI CTGCAG 2 cut(s) 886, 1637
PstNI CAGNNNCTG 1 cut(s) 1730
PsuI RGATCY 2 cut(s) 364, 779
PvuII CAGCTG 4 cut(s) 241, 451, 1037, 1334
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 9 cut(s) 92, 140, 158, 245, 257, 296, 329, 1016, 1552
RsaNI GTAC 9 cut(s) 91, 139, 157, 244, 256, 295, 328, 1015, 1551
RseI CAYNNNNRTG 2 cut(s) 705, 869
Rsr2I CGGWCCG 2 cut(s) 55, 435
RsrII CGGWCCG 2 cut(s) 55, 435
SapI GCTCTTC 1 cut(s) 1658
SaqAI TTAA 3 cut(s) 516, 1391, 1791
SatI GCNGC 9 cut(s) 146, 452, 590, 593, 752, 852, 882, 1335, 1607
Sau3AI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1594
Sau96I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
SchI GAGTC 4 cut(s) 381, 731, 870, 1166
ScrFI CCNGG 6 cut(s) 35, 77, 173, 503, 1230, 1377
SduI GDGCHC 4 cut(s) 105, 837, 1582, 1653
SfaNI GCATC 3 cut(s) 670, 1372, 1510
SfcI CTRYAG 2 cut(s) 882, 1633
SinI GGWCC 5 cut(s) 55, 73, 342, 435, 1193
SmiMI CAYNNNNRTG 2 cut(s) 705, 869
Sse9I AATT 8 cut(s) 496, 511, 543, 671, 716, 1239, 1451, 1711
SspI AATATT 1 cut(s) 1682
SspMI CTAG 4 cut(s) 182, 402, 530, 1197
StyD4I CCNGG 6 cut(s) 33, 75, 171, 501, 1228, 1375
StyI CCWWGG 1 cut(s) 917
TaaI ACNGT 5 cut(s) 1001, 1014, 1548, 1750, 1787
TaiI ACGT 5 cut(s) 18, 32, 84, 300, 1491
TaqI TCGA 2 cut(s) 186, 333
TaqII GACCGA 1 cut(s) 452
TasI AATT 8 cut(s) 496, 511, 543, 671, 716, 1239, 1451, 1711
TatI WGTACW 3 cut(s) 243, 255, 1550
TauI GCSGC 4 cut(s) 148, 592, 854, 1609
TfiI GAWTC 7 cut(s) 393, 614, 806, 1136, 1372, 1567, 1727
Tru1I TTAA 3 cut(s) 516, 1391, 1791
Tru9I TTAA 3 cut(s) 516, 1391, 1791
TscAI CASTG 3 cut(s) 690, 1006, 1483
TseFI GTSAC 2 cut(s) 946, 1280
TseI GCWGC 5 cut(s) 451, 592, 751, 881, 1334
Tsp45I GTSAC 2 cut(s) 946, 1280
TspDTI ATGAA 4 cut(s) 572, 593, 887, 1580
TspGWI ACGGA 1 cut(s) 1416
TspRI CASTG 3 cut(s) 690, 1006, 1483
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 5 cut(s) 55, 73, 342, 435, 1193
XapI RAATTY 2 cut(s) 671, 716
XceI RCATGY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 1696
XspI CTAG 4 cut(s) 182, 402, 530, 1197
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.