Rh2AG233700

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009505
Physical Location & Seq
Forward (+)
25195570 .. 25201722
6153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG233700.1

Sequence Viewer

Length: 1410 bp
ATGTCAGATAACGACGTTGTTTCCGGCACGTTCCGGGCGCTGGTGGAGGGCGCGGACCGGAAGTTCGCGAGGGTCCGGGACGTGCCGGCGTACGGGCGCGTGCACAACCAGCACTACTTTCACAAGGTGTTCAAGGCTTACATGCGGCTGTGGAAGTACCAGCAGGAGAACCGGGCCAAGCTTGTCGAGGCCGGTCTGAACCGGTGGGAAATCGGCGAGATCGCCAGCCGGATCGGCCAGTTGTACTTCGGCCAGTACATGAGGACGAGCGAGGCGAGGTTTCTGGCCGAGGCGTACGTCTTCTACGAGGCCATTCTGAGCCGGCGGTACTTCGAGGGAGGGTCCAAGAGTTTGGGGAAGAAGGATCTTGGAGTCCGGTTCAAGGAGTTGAGATTCTACGCTAGGTTCTTGCTGGTTTCGTTGATTTTGAACCGGACCGTGATGGTTCAGCTGCTCGCCGAGCGGTTCAAGGCTCTGGTTGATGACAGCAGAGCCAATTACCGGGAAAGTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGTGCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCACTGCGATACTCTGCCATGTTTGATTCACATCCATCGTCTCTCCCATATGTGGCTCGTTTCCATGCAAAGAGAGTTCTAAAATTTCATGATGCACTGCTAACAAGCTATCACCGGAATGAGGCCAAATTTGCTGAACTTACGTTGGACACATACCGAATGCTGCAATGTTTGGAATGGGAGCCAAGCGGATCTTTCTACCAAAAGCGCCCTGTTGAATCAAAAGAGAACGGCTCTTTTATTGAGCACTCTGGGGCTTCTGCGGCTTCTGGAGTCATTGATATGAACTTGGCTGCAGATATGACTGATCCAAGTTTACCTCCAAATCCAAGGAAAGCGGTTATCTATCGTCCATCTGTGACACATTTGATAGCGGTGATGGCAACAATATGTGAAGAACTCCCTGTGGACAGTGTTATGCTACTGTACCTGTCAGCCTCAGGGACGGCTGGTCGTAGTAATGTAAATCAGCTATACAACTCTGGAGGATCACAAAAATCGTCAAAAAATAAAGTTCCTCTCCCAGATCAGAATACTTCTCGGCCTGAATCTTGTAATGGCAAGGCAGAGTCAAGTGGCTATTATGATAAGTATCTGTGGTTTGGTCCTAGAGGAAATGGCGGTGAGAATAACCTTTACCCTGGAGACATAATTCCTTTTACTCGCAGACCTCTTTTCTTGATAGTTGATAGTGACAACAGCCATGCATTCAAGGCAGGTTTGTCAAAACTTTTGCATGACTTTAGACATACTTGGTTTCTGAAGCAGATATGGTGTCTGTATGAAAAATCACATTTTCATACTATATGCTTGATCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

469

Amino Acids

53.95

Weight (kDa)

9.45

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 14 - 435 7.6e-160 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1295
AccBSI CCGCTC 1 cut(s) 463
AccII CGCG 3 cut(s) 53, 68, 99
AciI CCGC 9 cut(s) 53, 145, 325, 463, 777, 851, 926, 962, 1209
AclWI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1396
AcoI YGGCCR 3 cut(s) 235, 250, 285
AcsI RAATTY 2 cut(s) 671, 716
AcuI CTGAAG 1 cut(s) 1370
AdeI CACNNNGTG 1 cut(s) 127
AfaI GTAC 7 cut(s) 92, 158, 245, 257, 296, 329, 1016
AfiI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
AgeI ACCGGT 1 cut(s) 201
AgsI TTSAA 6 cut(s) 133, 382, 430, 469, 806, 1300
AhdI GACNNNNNGTC 1 cut(s) 1038
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 1228
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 4 cut(s) 181, 451, 696, 1060
AluI AGCT 4 cut(s) 181, 451, 696, 1060
Alw21I GWGCWC 2 cut(s) 105, 837
Alw26I GTCTC 2 cut(s) 633, 1227
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1396
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 3 cut(s) 451, 751, 881
ApoI RAATTY 2 cut(s) 671, 716
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AsiGI ACCGGT 1 cut(s) 201
AspLEI GCGC 4 cut(s) 40, 53, 99, 798
AspS9I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
AsuC2I CCSGG 4 cut(s) 35, 77, 173, 503
AsuHPI GGTGA 3 cut(s) 692, 976, 1223
AvaII GGWCC 5 cut(s) 55, 73, 342, 435, 1193
AxyI CCTNAGG 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 105, 837
BbvI GCAGC 3 cut(s) 438, 738, 868
BccI CCATC 4 cut(s) 436, 631, 949, 961
BceAI ACGGC 3 cut(s) 585, 835, 1050
BciT130I CCWGG 1 cut(s) 1230
BcnI CCSGG 4 cut(s) 35, 77, 173, 503
BcoDI GTCTC 2 cut(s) 633, 1227
BfaI CTAG 3 cut(s) 402, 530, 1197
BfmI CTRYAG 2 cut(s) 882, 1406
BfoI RGCGCY 2 cut(s) 41, 799
BfuAI ACCTGC 1 cut(s) 1295
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 5 cut(s) 146, 452, 752, 852, 882
BlsI GCNGC 5 cut(s) 147, 453, 753, 853, 883
Bme1390I CCNGG 5 cut(s) 35, 77, 173, 503, 1230
Bme18I GGWCC 5 cut(s) 55, 73, 342, 435, 1193
BmeRI GACNNNNNGTC 1 cut(s) 1038
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
BmiI GGNNCC 3 cut(s) 74, 343, 771
BmrFI CCNGG 5 cut(s) 35, 77, 173, 503, 1230
BmsI GCATC 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpmI CTGGAG 3 cut(s) 879, 1092, 1251
BpuMI CCSGG 4 cut(s) 35, 77, 173, 503
BsaBI GATNNNNATC 2 cut(s) 618, 1179
BsaJI CCNNGG 3 cut(s) 288, 917, 1228
BsaWI WCCGGW 5 cut(s) 57, 201, 375, 432, 702
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
Bse118I RCCGGY 4 cut(s) 85, 191, 201, 321
Bse1I ACTGG 2 cut(s) 238, 253
Bse21I CCTNAGG 1 cut(s) 1027
Bse3DI GCAATG 1 cut(s) 761
Bse8I GATNNNNATC 2 cut(s) 618, 1179
BseBI CCWGG 1 cut(s) 1230
BseDI CCNNGG 3 cut(s) 288, 917, 1228
BseGI GGATG 1 cut(s) 619
BseJI GATNNNNATC 2 cut(s) 618, 1179
BseLI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
BseMI GCAATG 1 cut(s) 761
BseMII CTCAG 2 cut(s) 308, 1041
BseNI ACTGG 2 cut(s) 238, 253
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 3 cut(s) 438, 738, 868
Bsh1236I CGCG 3 cut(s) 53, 68, 99
BshTI ACCGGT 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 105, 837
BsiWI CGTACG 2 cut(s) 90, 294
BslFI GGGAC 2 cut(s) 92, 1045
BslI CCNNNNNNNGG 6 cut(s) 40, 92, 382, 501, 640, 709
BsmAI GTCTC 2 cut(s) 633, 1227
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 2 cut(s) 92, 1045
BsmI GAATGC 2 cut(s) 753, 1295
Bsp1286I GDGCHC 2 cut(s) 105, 837
Bsp143I GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
Bsp68I TCGCGA 1 cut(s) 68
BspACI CCGC 9 cut(s) 53, 145, 325, 463, 777, 851, 926, 962, 1209
BspCNI CTCAG 2 cut(s) 309, 1040
BspFNI CGCG 3 cut(s) 53, 68, 99
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 74, 343, 771
BspMAI CTGCAG 1 cut(s) 886
BspMI ACCTGC 1 cut(s) 1295
BspPI GGATC 6 cut(s) 239, 372, 787, 890, 1084, 1396
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 761
BsrFI RCCGGY 4 cut(s) 85, 191, 201, 321
BsrI ACTGG 2 cut(s) 238, 253
BssAI RCCGGY 4 cut(s) 85, 191, 201, 321
BssECI CCNNGG 3 cut(s) 288, 917, 1228
BssMI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
BssT1I CCWWGG 1 cut(s) 917
Bst2UI CCWGG 1 cut(s) 1230
Bst4CI ACNGT 3 cut(s) 439, 1001, 1014
BstC8I GCNNGC 5 cut(s) 87, 101, 226, 323, 456
BstDEI CTNAG 2 cut(s) 317, 1027
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 3 cut(s) 53, 68, 99
BstH2I RGCGCY 2 cut(s) 41, 799
BstHHI GCGC 4 cut(s) 40, 53, 99, 798
BstKTI GATC 8 cut(s) 222, 234, 367, 782, 898, 1079, 1117, 1404
BstMAI GTCTC 2 cut(s) 633, 1227
BstMBI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
BstMWI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1301
BstNI CCWGG 1 cut(s) 1230
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 5 cut(s) 33, 75, 171, 501, 1228
BstSFI CTRYAG 2 cut(s) 882, 1406
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 3 cut(s) 53, 68, 99
BstV1I GCAGC 3 cut(s) 438, 738, 868
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 2 cut(s) 364, 779
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 2 cut(s) 364, 779
Bsu36I CCTNAGG 1 cut(s) 1027
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 619
BtsI GCAGTG 2 cut(s) 590, 683
BtsIMutI CAGTG 3 cut(s) 590, 683, 1006
BtuMI TCGCGA 1 cut(s) 68
BveI ACCTGC 1 cut(s) 1295
Cac8I GCNNGC 5 cut(s) 87, 101, 226, 323, 456
CciI TCATGA 1 cut(s) 676
CfoI GCGC 4 cut(s) 40, 53, 99, 798
Cfr10I RCCGGY 4 cut(s) 85, 191, 201, 321
Cfr13I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
CpoI CGGWCCG 2 cut(s) 55, 435
Csp6I GTAC 7 cut(s) 91, 157, 244, 256, 295, 328, 1015
CspAI ACCGGT 1 cut(s) 201
CspI CGGWCCG 2 cut(s) 55, 435
CviAII CATG 7 cut(s) 142, 259, 607, 653, 677, 1292, 1325
CviQI GTAC 7 cut(s) 91, 157, 244, 256, 295, 328, 1015
DdeI CTNAG 2 cut(s) 317, 1027
DpnI GATC 8 cut(s) 221, 233, 366, 781, 897, 1078, 1116, 1403
DpnII GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
DraI TTTAAA 1 cut(s) 517
DraIII CACNNNGTG 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 1038
EaeI YGGCCR 3 cut(s) 235, 250, 285
Eam1105I GACNNNNNGTC 1 cut(s) 1038
Eco130I CCWWGG 1 cut(s) 917
Eco47I GGWCC 5 cut(s) 55, 73, 342, 435, 1193
Eco57I CTGAAG 1 cut(s) 1370
Eco81I CCTNAGG 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 1228
EcoT14I CCWWGG 1 cut(s) 917
EcoT22I ATGCAT 1 cut(s) 1297
ErhI CCWWGG 1 cut(s) 917
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 7 cut(s) 145, 262, 610, 656, 680, 1295, 1328
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 2 cut(s) 92, 1045
FatI CATG 7 cut(s) 141, 258, 606, 652, 676, 1291, 1324
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 5 cut(s) 146, 452, 752, 852, 882
FokI GGATG 1 cut(s) 606
Fsp4HI GCNGC 5 cut(s) 146, 452, 752, 852, 882
FspBI CTAG 3 cut(s) 402, 530, 1197
GlaI GCGC 4 cut(s) 39, 52, 98, 797
GluI GCNGC 5 cut(s) 146, 452, 752, 852, 882
GsuI CTGGAG 3 cut(s) 879, 1092, 1251
HaeII RGCGCY 2 cut(s) 41, 799
HhaI GCGC 4 cut(s) 40, 53, 99, 798
Hin1II CATG 7 cut(s) 145, 262, 610, 656, 680, 1295, 1328
Hin6I GCGC 4 cut(s) 38, 51, 97, 796
HinP1I GCGC 4 cut(s) 38, 51, 97, 796
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 7 cut(s) 372, 393, 614, 806, 861, 1136, 1157
HphI GGTGA 3 cut(s) 692, 976, 1223
Hpy166II GTNNAC 3 cut(s) 103, 905, 997
Hpy188I TCNGA 5 cut(s) 7, 198, 318, 1119, 1350
Hpy188III TCNNGA 5 cut(s) 67, 677, 858, 1071, 1267
Hpy8I GTNNAC 3 cut(s) 103, 905, 997
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 3 cut(s) 439, 1001, 1014
HpyCH4IV ACGT 5 cut(s) 15, 29, 81, 297, 731
HpyCH4V TGCA 8 cut(s) 103, 538, 656, 683, 754, 884, 1295, 1324
HpyF10VI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1301
HpyF3I CTNAG 2 cut(s) 317, 1027
HpySE526I ACGT 5 cut(s) 15, 29, 81, 297, 731
Hsp92II CATG 7 cut(s) 145, 262, 610, 656, 680, 1295, 1328
HspAI GCGC 4 cut(s) 38, 51, 97, 796
KroI GCCGGC 2 cut(s) 85, 321
KroNI GCCGGC 2 cut(s) 87, 323
Kzo9I GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 3 cut(s) 438, 738, 868
LweI GCATC 1 cut(s) 670
MaeI CTAG 3 cut(s) 402, 530, 1197
MaeII ACGT 5 cut(s) 15, 29, 81, 297, 731
MaeIII GTNAC 2 cut(s) 946, 1280
MalI GATC 8 cut(s) 221, 233, 366, 781, 897, 1078, 1116, 1403
MbiI CCGCTC 1 cut(s) 463
MboI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
MboII GAAGA 3 cut(s) 292, 370, 995
MflI RGATCY 2 cut(s) 364, 779
MhlI GDGCHC 2 cut(s) 105, 837
MluCI AATT 6 cut(s) 496, 511, 543, 671, 716, 1239
MlyI GAGTC 3 cut(s) 381, 870, 1166
MmeI TCCRAC 1 cut(s) 714
Mph1103I ATGCAT 1 cut(s) 1297
MroNI GCCGGC 2 cut(s) 85, 321
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 2 cut(s) 705, 869
MspA1I CMGCKG 1 cut(s) 451
MspR9I CCNGG 5 cut(s) 35, 77, 173, 503, 1230
Mva1269I GAATGC 2 cut(s) 753, 1295
MvaI CCWGG 1 cut(s) 1230
MvnI CGCG 3 cut(s) 53, 68, 99
MwoI GCNNNNNNNGC 8 cut(s) 109, 234, 460, 535, 719, 851, 968, 1301
NaeI GCCGGC 2 cut(s) 87, 323
NciI CCSGG 4 cut(s) 35, 77, 173, 503
NdeI CATATG 1 cut(s) 637
NdeII GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
NgoMIV GCCGGC 2 cut(s) 85, 321
NlaIII CATG 7 cut(s) 145, 262, 610, 656, 680, 1295, 1328
NlaIV GGNNCC 3 cut(s) 74, 343, 771
NmeAIII GCCGAG 3 cut(s) 313, 484, 1108
NmuCI GTSAC 2 cut(s) 946, 1280
NruI TCGCGA 1 cut(s) 68
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 145
PagI TCATGA 1 cut(s) 676
PcsI WCGNNNNNNNCGW 2 cut(s) 272, 303
PctI GAATGC 2 cut(s) 753, 1295
PdiI GCCGGC 2 cut(s) 87, 323
PfeI GAWTC 4 cut(s) 393, 614, 806, 1136
Pfl23II CGTACG 2 cut(s) 90, 294
PfoI TCCNGGA 1 cut(s) 75
PinAI ACCGGT 1 cut(s) 201
PkrI GCNGC 5 cut(s) 147, 453, 753, 853, 883
PleI GAGTC 3 cut(s) 380, 869, 1165
PpsI GAGTC 3 cut(s) 380, 869, 1165
Psp6I CCWGG 1 cut(s) 1228
PspGI CCWGG 1 cut(s) 1228
PspLI CGTACG 2 cut(s) 90, 294
PspN4I GGNNCC 3 cut(s) 74, 343, 771
PspPI GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
PstI CTGCAG 1 cut(s) 886
PsuI RGATCY 2 cut(s) 364, 779
PvuII CAGCTG 1 cut(s) 451
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 7 cut(s) 92, 158, 245, 257, 296, 329, 1016
RsaNI GTAC 7 cut(s) 91, 157, 244, 256, 295, 328, 1015
RseI CAYNNNNRTG 2 cut(s) 705, 869
Rsr2I CGGWCCG 2 cut(s) 55, 435
RsrII CGGWCCG 2 cut(s) 55, 435
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 5 cut(s) 146, 452, 752, 852, 882
Sau3AI GATC 8 cut(s) 219, 231, 364, 779, 895, 1076, 1114, 1401
Sau96I GGNCC 6 cut(s) 55, 73, 174, 342, 435, 1193
SchI GAGTC 3 cut(s) 381, 870, 1166
ScrFI CCNGG 5 cut(s) 35, 77, 173, 503, 1230
SduI GDGCHC 2 cut(s) 105, 837
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 2 cut(s) 882, 1406
SinI GGWCC 5 cut(s) 55, 73, 342, 435, 1193
SmiMI CAYNNNNRTG 2 cut(s) 705, 869
Sse9I AATT 6 cut(s) 496, 511, 543, 671, 716, 1239
SsiI CCGC 9 cut(s) 53, 145, 325, 463, 777, 851, 926, 962, 1209
SspMI CTAG 3 cut(s) 402, 530, 1197
StyD4I CCNGG 5 cut(s) 33, 75, 171, 501, 1228
StyI CCWWGG 1 cut(s) 917
TaaI ACNGT 3 cut(s) 439, 1001, 1014
TaiI ACGT 5 cut(s) 18, 32, 84, 300, 734
TaqI TCGA 2 cut(s) 186, 333
TasI AATT 6 cut(s) 496, 511, 543, 671, 716, 1239
TatI WGTACW 2 cut(s) 243, 255
TauI GCSGC 2 cut(s) 148, 854
TfiI GAWTC 4 cut(s) 393, 614, 806, 1136
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 3 cut(s) 597, 690, 1006
TseFI GTSAC 2 cut(s) 946, 1280
TseI GCWGC 3 cut(s) 451, 751, 881
Tsp45I GTSAC 2 cut(s) 946, 1280
TspDTI ATGAA 6 cut(s) 572, 593, 665, 887, 1376, 1386
TspRI CASTG 3 cut(s) 597, 690, 1006
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 5 cut(s) 55, 73, 342, 435, 1193
XapI RAATTY 2 cut(s) 671, 716
XceI RCATGY 1 cut(s) 145
XspI CTAG 3 cut(s) 402, 530, 1197
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.