MD01G1035400.v1.1

Protein SCAI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009505
Physical Location & Seq
Reverse (-)
11990053 .. 11996265
6213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1035400.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGTCCGAAAACGACGTCGTGAAGCGGACGTTCCGGGCGCTGGTGGAGGGCGCCGACCGGAAGTTCGCTAGGGTTCGCGATGTGCCGGCGTACGGACGCCTCCCCAACCAGCACTACTTCCACAAGGTCTTCAAGGCCTACATGCGCCTCTGGAAGTACCAGCAGGAGAACCGCTCCAAGCTCGTCGGCGCCGGTCTGAACCGCTGGGAAATCGGCGAGATCGCCAGCCGGATCGGCCAGCTCTACTTCGGCCAGTATATGCGGACCAGCGAGGCCCGATTCCTCATCGAGGCCTACGTCTTCTACGAGGCCATTCTCAGCCGCAACTACTTCGACGGCTCCGCCGGCTCCAGAGGTTTTGGGAAGAAGGATATCGGAGTCCGGTTCAAGGAGCTGAGGTTCTACGCTCGGTTTTTGCTCGTTTCGTTGATTTTGAACCGGACCGAGACAGTTCAGCTTCTTGCTGAGCGGTTCAAATCGCTTGTTGACAACTGCAAAGCCAATTTTCGGGACACTAATTTTAAAGAATGGAGGCTAGTGGTGCAAGAAATTGCTCGATTTATGAAAGTTGATACGGCCTTTATGAATGTCAGGCCATTGCGATACTCCACCTTGTTTGATTCACATCCAGCATCTCTTCAATATGTTACTCGTTTCCATGCAAAGAGAGTTTTAAAATTTCAAGATGCATTGTTGACAAGCTATCACCATAATGAGGTCAAATTTGCTGAACTTACATTGGACACTTTCCGAATGCTGCAATGTTTGGAATGGGAGCCAAGTGGGTCTTTCTACCAAAAGCGCCCAGTTGAACCAAAGGAGAATGGCGTTTTTATTGAACACTCTGGGGCTTCTGCAGCATCTGGTGTAATTGATTTGAACTTGGCTGCGGACATGACTGATCCAAGTTTACCACCAAATCCCAGGAAAGCTGTTATCTATCGTCCATCTGTGACACATTTGATAGCGGTGATGGCTACAATTTGTGAGGAACTCCCTCTGGACAGTATTATGCTAGTGTATCTGTCAGCCTCAGGGAAGGTTGATCACAGTAATACTAGTCAGGTGTACAACTCTGGGGGATCACAGAAGTCCTTAAAAAACAAAGTTCCTCTCCAAGATCAGAACAAGTTTCAGCCTGAATCACGTATAAATAACAAGGGAGAGTCAAGTGGCTATTATGATCAATATCTATGGTTTGGCCCTAGAGGAAATGGTGGTTTGAATAACCTTTACCCCGGTGATATAATTCCTTTTACTCGTAGACCACTTTTCTTGGTAATAGATAGCGACAACAGCCATGCATTCAAGGTTTTACATGGAGCGGAAAGAGGAGAGACGGCTGCTCTGCTTCTTTCACCTATGAGACCAGCATTCAAGAATTCAGCTGATGCTGATTTAACGCAAAATGGAAGTCAGTTTACCTTTTTCTTGACTGCTCCCCTGGCAGCTTTCAGCCAATTAGTTGGCTTCTCCTCTTCTGATACTGAAGCAGAGGTTTATAGCAATGCTGAAAGCCTACTCTCTGCTGCCTTCTCTGAGTGGGAAGTTATTCTTTGTACAACGACAAGCCTGGATCTGGTATGGGCACAAGTTATATCCGATCCTTTTTTGCGGCGACTTATTCTGAGATTTATATTCTGCAGAGCAGTGCTTTATTTCTTCTGTCCTCCTGAAGACAGCGAGCAGCATTTGCCGGTTTGCCTTCCTCTTCTCCCCAATTCAGTTTCTGCAGATTCTGAAGTTGTGCGGTCTGCTGTCCAGAGGCTTGCGAAGCACCTCAGCGTCTCCGACTTATTTCACCTTGTTGAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SCAI

Protein Analysis

606

Amino Acids

68.77

Weight (kDa)

8.73

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAI PF12070 14 - 556 3.9e-199 Protein SCAI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AccB1I GGYRCC 2 cut(s) 50, 188
AccBSI CCGCTC 3 cut(s) 174, 469, 1325
AccI GTMKAC 1 cut(s) 1264
AccII CGCG 1 cut(s) 78
AclWI GGATC 5 cut(s) 239, 896, 1090, 1584, 1598
AcoI YGGCCR 2 cut(s) 235, 250
AcsI RAATTY 3 cut(s) 677, 722, 1381
AcuI CTGAAG 3 cut(s) 1509, 1695, 1761
AcyI GRCGYC 4 cut(s) 15, 51, 97, 189
AfaI GTAC 4 cut(s) 92, 158, 1070, 1561
AfiI CCNNNNNNNGG 7 cut(s) 40, 92, 289, 388, 507, 715, 1579
AhlI ACTAGT 1 cut(s) 1058
AjnI CCWGG 3 cut(s) 923, 1443, 1572
AloI GAACNNNNNNTCC 2 cut(s) 383, 415
AluBI AGCT 8 cut(s) 181, 241, 394, 457, 702, 932, 1388, 1451
AluI AGCT 8 cut(s) 181, 241, 394, 457, 702, 932, 1388, 1451
Alw26I GTCTC 5 cut(s) 440, 1331, 1360, 1792, 1805
AlwI GGATC 5 cut(s) 239, 896, 1090, 1584, 1598
AlwNI CAGNNNCTG 3 cut(s) 863, 1730, 1739
AoxI GGCC 9 cut(s) 135, 235, 250, 273, 291, 309, 576, 593, 1201
ApeKI GCWGC 7 cut(s) 757, 857, 887, 1343, 1448, 1529, 1687
ApoI RAATTY 3 cut(s) 677, 722, 1381
ArsI GACNNNNNNTTYG 5 cut(s) 32, 47, 79, 1399, 1431
Asp700I GAANNNNTTC 1 cut(s) 1551
AspLEI GCGC 5 cut(s) 40, 53, 147, 191, 804
AspS9I GGNCC 4 cut(s) 264, 274, 441, 1202
AsuC2I CCSGG 2 cut(s) 35, 1239
AsuHPI GGTGA 5 cut(s) 698, 982, 1253, 1350, 1793
AvaII GGWCC 2 cut(s) 264, 441
AxyI CCTNAGG 1 cut(s) 1033
BaeGI GKGCMC 1 cut(s) 1591
BanI GGYRCC 2 cut(s) 50, 188
BarI GAAGNNNNNNTAC 2 cut(s) 1405, 1437
BbsI GAAGAC 3 cut(s) 121, 292, 1683
BbvCI CCTCAGC 2 cut(s) 395, 1781
BbvI GCAGC 7 cut(s) 744, 869, 874, 1330, 1460, 1516, 1699
BccI CCATC 2 cut(s) 955, 967
BceAI ACGGC 3 cut(s) 352, 591, 1356
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 3 cut(s) 925, 1445, 1574
BclI TGATCA 2 cut(s) 1045, 1183
BcnI CCSGG 2 cut(s) 35, 1239
BcoDI GTCTC 5 cut(s) 440, 1331, 1360, 1792, 1805
BcuI ACTAGT 1 cut(s) 1058
BfaI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
BfmI CTRYAG 3 cut(s) 855, 1642, 1731
BfoI RGCGCY 4 cut(s) 41, 54, 192, 805
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 9 cut(s) 322, 758, 858, 888, 1344, 1449, 1530, 1616, 1688
BlpI GCTNAGC 1 cut(s) 465
BlsI GCNGC 9 cut(s) 323, 759, 859, 889, 1345, 1450, 1531, 1617, 1689
Bme1390I CCNGG 5 cut(s) 35, 925, 1239, 1445, 1574
Bme18I GGWCC 2 cut(s) 264, 441
BmgT120I GGNCC 4 cut(s) 264, 274, 441, 1202
BmiI GGNNCC 5 cut(s) 52, 190, 340, 349, 777
BmrFI CCNGG 5 cut(s) 35, 925, 1239, 1445, 1574
BmrI ACTGGG 1 cut(s) 800
BmsI GCATC 4 cut(s) 641, 676, 869, 1381
BmuI ACTGGG 1 cut(s) 800
BpiI GAAGAC 3 cut(s) 121, 292, 1683
BplI GAGNNNNNCTC 2 cut(s) 158, 190
BpmI CTGGAG 1 cut(s) 334
Bpu10I CCTNAGC 2 cut(s) 395, 1781
Bpu1102I GCTNAGC 1 cut(s) 465
BpuMI CCSGG 2 cut(s) 35, 1239
BsaAI YACGTR 1 cut(s) 1148
BsaBI GATNNNNATC 2 cut(s) 624, 1188
BsaHI GRCGYC 4 cut(s) 15, 51, 97, 189
BsaI GGTCTC 1 cut(s) 1360
BsaJI CCNNGG 3 cut(s) 923, 1237, 1443
BsaWI WCCGGW 3 cut(s) 57, 381, 438
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 92, 289, 388, 507, 715, 1579
Bse118I RCCGGY 4 cut(s) 85, 191, 344, 1696
Bse1I ACTGG 2 cut(s) 253, 806
Bse21I CCTNAGG 1 cut(s) 1033
Bse3DI GCAATG 3 cut(s) 596, 767, 1513
Bse8I GATNNNNATC 2 cut(s) 624, 1188
BseBI CCWGG 3 cut(s) 925, 1445, 1574
BseDI CCNNGG 3 cut(s) 923, 1237, 1443
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 2 cut(s) 624, 1188
BseLI CCNNNNNNNGG 7 cut(s) 40, 92, 289, 388, 507, 715, 1579
BseMI GCAATG 3 cut(s) 596, 767, 1513
BseMII CTCAG 7 cut(s) 331, 386, 456, 1047, 1530, 1619, 1795
BseNI ACTGG 2 cut(s) 253, 806
BseRI GAGGAG 2 cut(s) 1347, 1465
BseSI GKGCMC 1 cut(s) 1591
BseXI GCAGC 7 cut(s) 744, 869, 874, 1330, 1460, 1516, 1699
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 9 cut(s) 137, 237, 252, 275, 293, 311, 578, 595, 1203
BshNI GGYRCC 2 cut(s) 50, 188
BsiEI CGRYCG 1 cut(s) 58
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 7 cut(s) 40, 92, 289, 388, 507, 715, 1579
BsmAI GTCTC 5 cut(s) 440, 1331, 1360, 1792, 1805
BsmBI CGTCTC 2 cut(s) 1331, 1792
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 3 cut(s) 759, 1304, 1373
BsnI GGCC 9 cut(s) 137, 237, 252, 275, 293, 311, 578, 595, 1203
Bso31I GGTCTC 1 cut(s) 1360
Bsp1286I GDGCHC 1 cut(s) 1591
Bsp1407I TGTACA 2 cut(s) 1068, 1559
Bsp143I GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
Bsp1720I GCTNAGC 1 cut(s) 465
Bsp68I TCGCGA 1 cut(s) 78
BspANI GGCC 9 cut(s) 137, 237, 252, 275, 293, 311, 578, 595, 1203
BspCNI CTCAG 7 cut(s) 330, 387, 457, 1046, 1531, 1620, 1794
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 5 cut(s) 52, 190, 340, 349, 777
BspMAI CTGCAG 3 cut(s) 859, 1646, 1735
BspPI GGATC 5 cut(s) 239, 896, 1090, 1584, 1598
BspT107I GGYRCC 2 cut(s) 50, 188
BspTNI GGTCTC 1 cut(s) 1360
BsrBI CCGCTC 3 cut(s) 174, 469, 1325
BsrDI GCAATG 3 cut(s) 596, 767, 1513
BsrFI RCCGGY 4 cut(s) 85, 191, 344, 1696
BsrGI TGTACA 2 cut(s) 1068, 1559
BsrI ACTGG 2 cut(s) 253, 806
BssAI RCCGGY 4 cut(s) 85, 191, 344, 1696
BssECI CCNNGG 3 cut(s) 923, 1237, 1443
BssMI GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
BssNI GRCGYC 4 cut(s) 15, 51, 97, 189
Bst2UI CCWGG 3 cut(s) 925, 1445, 1574
Bst4CI ACNGT 3 cut(s) 451, 1007, 1052
Bst6I CTCTTC 3 cut(s) 642, 1483, 1716
BstACI GRCGYC 4 cut(s) 15, 51, 97, 189
BstAPI GCANNNNNTGC 1 cut(s) 1693
BstAUI TGTACA 2 cut(s) 1068, 1559
BstBAI YACGTR 1 cut(s) 1148
BstC8I GCNNGC 6 cut(s) 87, 226, 239, 346, 1685, 1770
BstDEI CTNAG 7 cut(s) 317, 395, 465, 1033, 1539, 1628, 1781
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 625
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 4 cut(s) 41, 54, 192, 805
BstHHI GCGC 5 cut(s) 40, 53, 147, 191, 804
BstKTI GATC 9 cut(s) 222, 234, 904, 1048, 1085, 1123, 1186, 1579, 1606
BstMAI GTCTC 5 cut(s) 440, 1331, 1360, 1792, 1805
BstMBI GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 9 cut(s) 234, 345, 541, 857, 974, 1296, 1445, 1693, 1774
BstNI CCWGG 3 cut(s) 925, 1445, 1574
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 5 cut(s) 33, 923, 1237, 1443, 1572
BstSFI CTRYAG 3 cut(s) 855, 1642, 1731
BstSLI GKGCMC 1 cut(s) 1591
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 7 cut(s) 744, 869, 874, 1330, 1460, 1516, 1699
BstV2I GAAGAC 3 cut(s) 121, 292, 1683
BstX2I RGATCY 1 cut(s) 1576
BstXI CCANNNNNNTGG 1 cut(s) 1466
BstYI RGATCY 1 cut(s) 1576
Bsu36I CCTNAGG 1 cut(s) 1033
BsuRI GGCC 9 cut(s) 137, 237, 252, 275, 293, 311, 578, 595, 1203
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 1 cut(s) 1656
BtsIMutI CAGTG 1 cut(s) 1656
BtuMI TCGCGA 1 cut(s) 78
Cac8I GCNNGC 6 cut(s) 87, 226, 239, 346, 1685, 1770
CaiI CAGNNNCTG 3 cut(s) 863, 1730, 1739
CfoI GCGC 5 cut(s) 40, 53, 147, 191, 804
Cfr10I RCCGGY 4 cut(s) 85, 191, 344, 1696
Cfr13I GGNCC 4 cut(s) 264, 274, 441, 1202
CpoI CGGWCCG 1 cut(s) 441
CseI GACGC 2 cut(s) 105, 1774
Csp6I GTAC 4 cut(s) 91, 157, 1069, 1560
CspCI CAANNNNNGTGG 4 cut(s) 598, 633, 1257, 1292
CspI CGGWCCG 1 cut(s) 441
CviAII CATG 6 cut(s) 142, 659, 895, 1301, 1319, 1815
CviQI GTAC 4 cut(s) 91, 157, 1069, 1560
DdeI CTNAG 7 cut(s) 317, 395, 465, 1033, 1539, 1628, 1781
DinI GGCGCC 2 cut(s) 52, 190
DpnI GATC 9 cut(s) 221, 233, 903, 1047, 1084, 1122, 1185, 1578, 1605
DpnII GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
DraI TTTAAA 2 cut(s) 523, 675
EaeI YGGCCR 2 cut(s) 235, 250
Eam1104I CTCTTC 3 cut(s) 642, 1483, 1716
EarI CTCTTC 3 cut(s) 642, 1483, 1716
EciI GGCGGA 1 cut(s) 331
Eco147I AGGCCT 2 cut(s) 137, 293
Eco31I GGTCTC 1 cut(s) 1360
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 264, 441
Eco57I CTGAAG 3 cut(s) 1509, 1695, 1761
Eco81I CCTNAGG 1 cut(s) 1033
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 1381
EcoRII CCWGG 3 cut(s) 923, 1443, 1572
EcoRV GATATC 1 cut(s) 373
EcoT22I ATGCAT 2 cut(s) 691, 1306
EgeI GGCGCC 2 cut(s) 52, 190
EheI GGCGCC 2 cut(s) 52, 190
Esp3I CGTCTC 2 cut(s) 1331, 1792
FaeI CATG 6 cut(s) 145, 662, 898, 1304, 1322, 1818
FalI AAGNNNNNCTT 4 cut(s) 772, 804, 1539, 1571
FaqI GGGAC 1 cut(s) 524
FatI CATG 6 cut(s) 141, 658, 894, 1300, 1318, 1814
FbaI TGATCA 2 cut(s) 1045, 1183
FblI GTMKAC 1 cut(s) 1264
Fnu4HI GCNGC 9 cut(s) 322, 758, 858, 888, 1344, 1449, 1530, 1616, 1688
FokI GGATG 1 cut(s) 612
Fsp4HI GCNGC 9 cut(s) 322, 758, 858, 888, 1344, 1449, 1530, 1616, 1688
FspBI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
GlaI GCGC 5 cut(s) 39, 52, 146, 190, 803
GluI GCNGC 9 cut(s) 322, 758, 858, 888, 1344, 1449, 1530, 1616, 1688
GsaI CCCAGC 1 cut(s) 208
GsuI CTGGAG 1 cut(s) 334
HaeII RGCGCY 4 cut(s) 41, 54, 192, 805
HaeIII GGCC 9 cut(s) 137, 237, 252, 275, 293, 311, 578, 595, 1203
HgaI GACGC 2 cut(s) 105, 1774
HhaI GCGC 5 cut(s) 40, 53, 147, 191, 804
Hin1I GRCGYC 4 cut(s) 15, 51, 97, 189
Hin1II CATG 6 cut(s) 145, 662, 898, 1304, 1322, 1818
Hin6I GCGC 5 cut(s) 38, 51, 145, 189, 802
HinP1I GCGC 5 cut(s) 38, 51, 145, 189, 802
HincII GTYRAC 2 cut(s) 487, 696
HindII GTYRAC 2 cut(s) 487, 696
HinfI GANTC 6 cut(s) 279, 378, 620, 1142, 1166, 1736
HphI GGTGA 5 cut(s) 698, 982, 1253, 1350, 1793
Hpy166II GTNNAC 6 cut(s) 487, 696, 911, 1069, 1265, 1422
Hpy8I GTNNAC 6 cut(s) 487, 696, 911, 1069, 1265, 1422
Hpy99I CGWCG 4 cut(s) 17, 20, 188, 338
HpyAV CCTTC 4 cut(s) 361, 1033, 1543, 1715
HpyCH4III ACNGT 3 cut(s) 451, 1007, 1052
HpyCH4IV ACGT 4 cut(s) 15, 29, 297, 1147
HpyCH4V TGCA 9 cut(s) 495, 544, 662, 689, 760, 857, 1304, 1644, 1733
HpyF10VI GCNNNNNNNGC 9 cut(s) 234, 345, 541, 857, 974, 1296, 1445, 1693, 1774
HpyF3I CTNAG 7 cut(s) 317, 395, 465, 1033, 1539, 1628, 1781
HpySE526I ACGT 4 cut(s) 15, 29, 297, 1147
Hsp92I GRCGYC 4 cut(s) 15, 51, 97, 189
Hsp92II CATG 6 cut(s) 145, 662, 898, 1304, 1322, 1818
HspAI GCGC 5 cut(s) 38, 51, 145, 189, 802
KasI GGCGCC 2 cut(s) 50, 188
KroI GCCGGC 2 cut(s) 85, 344
KroNI GCCGGC 2 cut(s) 87, 346
Ksp22I TGATCA 2 cut(s) 1045, 1183
Kzo9I GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
LmnI GCTCC 7 cut(s) 179, 344, 353, 391, 775, 1322, 1444
Lsp1109I GCAGC 7 cut(s) 744, 869, 874, 1330, 1460, 1516, 1699
LweI GCATC 4 cut(s) 641, 676, 869, 1381
MaeI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
MaeII ACGT 4 cut(s) 15, 29, 297, 1147
MaeIII GTNAC 2 cut(s) 646, 952
MalI GATC 9 cut(s) 221, 233, 903, 1047, 1084, 1122, 1185, 1578, 1605
MbiI CCGCTC 3 cut(s) 174, 469, 1325
MboI GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
MboII GAAGA 8 cut(s) 121, 292, 376, 629, 1470, 1654, 1688, 1703
MflI RGATCY 1 cut(s) 1576
MhlI GDGCHC 1 cut(s) 1591
Mly113I GGCGCC 2 cut(s) 51, 189
MlyI GAGTC 2 cut(s) 387, 1175
MmeI TCCRAC 1 cut(s) 1815
Mph1103I ATGCAT 2 cut(s) 691, 1306
MroNI GCCGGC 2 cut(s) 85, 344
MroXI GAANNNNTTC 1 cut(s) 1551
MseI TTAA 4 cut(s) 522, 674, 1097, 1400
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 2 cut(s) 204, 1388
MspR9I CCNGG 5 cut(s) 35, 925, 1239, 1445, 1574
Mva1269I GAATGC 3 cut(s) 759, 1304, 1373
MvaI CCWGG 3 cut(s) 925, 1445, 1574
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 9 cut(s) 234, 345, 541, 857, 974, 1296, 1445, 1693, 1774
NaeI GCCGGC 2 cut(s) 87, 346
NarI GGCGCC 2 cut(s) 51, 189
NciI CCSGG 2 cut(s) 35, 1239
NdeII GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
NgoMIV GCCGGC 2 cut(s) 85, 344
NlaIII CATG 6 cut(s) 145, 662, 898, 1304, 1322, 1818
NlaIV GGNNCC 5 cut(s) 52, 190, 340, 349, 777
NmuCI GTSAC 1 cut(s) 952
NruI TCGCGA 1 cut(s) 78
NsiI ATGCAT 2 cut(s) 691, 1306
NspI RCATGY 1 cut(s) 145
PceI AGGCCT 2 cut(s) 137, 293
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 303
PctI GAATGC 3 cut(s) 759, 1304, 1373
PdiI GCCGGC 2 cut(s) 87, 346
PdmI GAANNNNTTC 1 cut(s) 1551
PfeI GAWTC 4 cut(s) 279, 620, 1142, 1736
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 9 cut(s) 323, 759, 859, 889, 1345, 1450, 1531, 1617, 1689
PleI GAGTC 2 cut(s) 386, 1174
PluTI GGCGCC 2 cut(s) 54, 192
PpsI GAGTC 2 cut(s) 386, 1174
Ppu21I YACGTR 1 cut(s) 1148
Psp6I CCWGG 3 cut(s) 923, 1443, 1572
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 3 cut(s) 923, 1443, 1572
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 52, 190, 340, 349, 777
PspPI GGNCC 4 cut(s) 264, 274, 441, 1202
PstI CTGCAG 3 cut(s) 859, 1646, 1735
PstNI CAGNNNCTG 3 cut(s) 863, 1730, 1739
PsuI RGATCY 1 cut(s) 1576
PvuII CAGCTG 1 cut(s) 1388
RruI TCGCGA 1 cut(s) 78
RsaI GTAC 4 cut(s) 92, 158, 1070, 1561
RsaNI GTAC 4 cut(s) 91, 157, 1069, 1560
RseI CAYNNNNRTG 1 cut(s) 711
Rsr2I CGGWCCG 1 cut(s) 441
RsrII CGGWCCG 1 cut(s) 441
SaqAI TTAA 4 cut(s) 522, 674, 1097, 1400
SatI GCNGC 9 cut(s) 322, 758, 858, 888, 1344, 1449, 1530, 1616, 1688
Sau3AI GATC 9 cut(s) 219, 231, 901, 1045, 1082, 1120, 1183, 1576, 1603
Sau96I GGNCC 4 cut(s) 264, 274, 441, 1202
SchI GAGTC 2 cut(s) 387, 1175
ScrFI CCNGG 5 cut(s) 35, 925, 1239, 1445, 1574
SduI GDGCHC 1 cut(s) 1591
SfaNI GCATC 4 cut(s) 641, 676, 869, 1381
SfcI CTRYAG 3 cut(s) 855, 1642, 1731
SfoI GGCGCC 2 cut(s) 52, 190
SinI GGWCC 2 cut(s) 264, 441
SmiMI CAYNNNNRTG 1 cut(s) 711
SpeI ACTAGT 1 cut(s) 1058
SseBI AGGCCT 2 cut(s) 137, 293
SspDI GGCGCC 2 cut(s) 50, 188
SspMI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
StuI AGGCCT 2 cut(s) 137, 293
StyD4I CCNGG 5 cut(s) 33, 923, 1237, 1443, 1572
TaaI ACNGT 3 cut(s) 451, 1007, 1052
TaiI ACGT 4 cut(s) 18, 32, 300, 1150
TaqI TCGA 3 cut(s) 288, 333, 556
TaqII GACCGA 1 cut(s) 458
TatI WGTACW 2 cut(s) 1068, 1559
TauI GCSGC 2 cut(s) 324, 1618
TfiI GAWTC 4 cut(s) 279, 620, 1142, 1736
Tru1I TTAA 4 cut(s) 522, 674, 1097, 1400
Tru9I TTAA 4 cut(s) 522, 674, 1097, 1400
TscAI CASTG 1 cut(s) 1656
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 7 cut(s) 757, 857, 887, 1343, 1448, 1529, 1687
Tsp45I GTSAC 1 cut(s) 952
TspDTI ATGAA 2 cut(s) 578, 599
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 1 cut(s) 1656
VpaK11BI GGWCC 2 cut(s) 264, 441
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 3 cut(s) 677, 722, 1381
XceI RCATGY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1551
XspI CTAG 5 cut(s) 69, 536, 1016, 1059, 1206
ZraI GACGTC 1 cut(s) 16
Zsp2I ATGCAT 2 cut(s) 691, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.