FvH4_1g20560

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013513
Physical Location & Seq
Forward (+)
12766012 .. 12767991
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20560.t1

Sequence Viewer

Length: 372 bp
ATGCGTTTCTCATCAGTGATTTCTTTTTTCACCATCTCCATGAATTCTTCAATAAATCCCAAGTTTTTCACCGCAACCAGAGCAACTATTGTTCTTCTTCTCCTTCTTCTTGTTCTCTCTCCAGCCTCTTGTTTTACTTCTCTTCGGGGACTGTTGTTTGCTGAGAAAGCCAGATTGGGCTCTAAACCGCCGAGCTGCCACAACAAGTGCAACCAGTGCCACCCGTGCATGGCCGTTCAAGTCCCCACCATGCCCAGCCACGACCGGGTCAAACCCGTGGGGAAGACCCGACCCGCTAAGCCCATGATGCTCTTTGACCCGGCTCATCGGGATAACAGAGAAGGCTTCAGAATTCAAGGAGTGACGCTATGA

Protein Analysis

124

Amino Acids

13.66

Weight (kDa)

10.39

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 58 - 86 4e-11 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 72, 188, 294
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 43, 351
AcuI CTGAAG 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 229, 265
AgsI TTSAA 3 cut(s) 51, 239, 356
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 1 cut(s) 195
AluI AGCT 1 cut(s) 195
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 2 cut(s) 43, 351
AsuC2I CCSGG 2 cut(s) 266, 320
AsuHPI GGTGA 2 cut(s) 22, 61
BanII GRGCYC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 41
BceAI ACGGC 1 cut(s) 218
BcnI CCSGG 2 cut(s) 266, 320
BisI GCNGC 1 cut(s) 196
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 197
Bme1390I CCNGG 2 cut(s) 266, 320
BmrFI CCNGG 2 cut(s) 266, 320
BmsI GCATC 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 105
Bpu1102I GCTNAGC 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 266, 320
BsaJI CCNNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 265
Bse1I ACTGG 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 276
BseLI CCNNNNNNNGG 2 cut(s) 229, 265
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 182
BseYI CCCAGC 1 cut(s) 254
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 265
BsiSI CCGG 2 cut(s) 265, 320
BslFI GGGAC 2 cut(s) 162, 227
BslI CCNNNNNNNGG 2 cut(s) 229, 265
BsmFI GGGAC 2 cut(s) 162, 227
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 182
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 3 cut(s) 72, 188, 294
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 1 cut(s) 154
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 162, 297
BstDSI CCRYGG 1 cut(s) 276
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 5 cut(s) 80, 167, 216, 225, 307
BstSCI CCNGG 2 cut(s) 264, 318
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 290
BsuRI GGCC 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 21, 221
CviAII CATG 4 cut(s) 40, 229, 250, 304
CviJI RGCY 9 cut(s) 125, 170, 180, 195, 233, 258, 301, 323, 345
CviKI_1 RGCY 9 cut(s) 125, 170, 180, 195, 233, 258, 301, 323, 345
DdeI CTNAG 2 cut(s) 162, 297
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 331
EcoRI GAATTC 2 cut(s) 43, 351
EcoT38I GRGCYC 1 cut(s) 182
FaeI CATG 4 cut(s) 43, 232, 253, 307
FaiI YATR 5 cut(s) 41, 230, 251, 305, 370
FaqI GGGAC 2 cut(s) 162, 227
FatI CATG 4 cut(s) 39, 228, 249, 303
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 1 cut(s) 196
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 196
GluI GCNGC 1 cut(s) 196
GsaI CCCAGC 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 1 cut(s) 233
HapII CCGG 2 cut(s) 265, 320
Hin1II CATG 4 cut(s) 43, 232, 253, 307
HpaII CCGG 2 cut(s) 265, 320
HphI GGTGA 2 cut(s) 22, 61
Hpy188I TCNGA 1 cut(s) 350
Hpy188III TCNNGA 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 113, 335
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 210, 228
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 167, 216, 225, 307
HpyF3I CTNAG 2 cut(s) 162, 297
Hsp92II CATG 4 cut(s) 43, 232, 253, 307
LpnPI CCDG 7 cut(s) 91, 135, 184, 227, 268, 278, 333
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 361
MboII GAAGA 6 cut(s) 39, 86, 89, 98, 134, 295
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 2 cut(s) 43, 351
MnlI CCTC 1 cut(s) 136
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 2 cut(s) 265, 320
MspR9I CCNGG 2 cut(s) 266, 320
MwoI GCNNNNNNNGC 5 cut(s) 80, 167, 216, 225, 307
NciI CCSGG 2 cut(s) 266, 320
NlaIII CATG 4 cut(s) 43, 232, 253, 307
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 361
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 254
PsyI GACNNNGTC 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 38
SatI GCNGC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 266, 320
SduI GDGCHC 1 cut(s) 182
SetI ASST 1 cut(s) 197
SfaNI GCATC 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 38
Sse9I AATT 2 cut(s) 43, 351
SsiI CCGC 3 cut(s) 72, 188, 294
StyD4I CCNGG 2 cut(s) 264, 318
TaaI ACNGT 1 cut(s) 153
TasI AATT 2 cut(s) 43, 351
TscAI CASTG 2 cut(s) 21, 221
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 1 cut(s) 56
TspRI CASTG 2 cut(s) 21, 221
Tth111I GACNNNGTC 1 cut(s) 266
XapI RAATTY 2 cut(s) 43, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.