RLG00000018009

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013513
Physical Location & Seq
Forward (+)
24383591 .. 24384047
457 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018009

Sequence Viewer

Length: 363 bp
ATGAATTCTTCAATAAATCTTCGGTTTTTCACCGCAACCAGAGCCATTATTGTTCTTCTTCTTCTTCTTCTTCTTCTCTCTCCAGCCTCTTGTTTTACTTCTCTTCGGGGACTATTGTTTGCTGAGAAAGCCAGATTGGGCTCAACACCGCCGAGCTGCCACAACAAGTGCAACCAGTGCCACCCTTGCATGGCGGTCCAAGTCCCCACCATGCCCAGCCGCGACCGGGTCCGACCCGTGGGGAAGACCCGACCCGCTAAGCCCATGATGCTCTTTGACACATCTCATCTGGATAACAGGTACTCCAATTACAAGCCACTGGGATGGAAATGCCACTGTGGTGACCACTTCTTCAATCCCTAA

Protein Analysis

121

Amino Acids

13.52

Weight (kDa)

9.77

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 45 - 120 1.9e-22 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 222
AciI CCGC 5 cut(s) 33, 149, 194, 220, 255
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 190, 226, 238
AgsI TTSAA 2 cut(s) 12, 355
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AleI CACNNNNGTG 1 cut(s) 339
AluBI AGCT 1 cut(s) 156
AluI AGCT 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 156
ApoI RAATTY 1 cut(s) 4
AspS9I GGNCC 2 cut(s) 196, 229
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 22, 353
AvaII GGWCC 2 cut(s) 196, 229
BanII GRGCYC 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 143
BccI CCATC 1 cut(s) 318
BcnI CCSGG 1 cut(s) 227
BisI GCNGC 2 cut(s) 157, 220
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 158, 221
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 2 cut(s) 196, 229
BmgT120I GGNCC 2 cut(s) 196, 229
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 226, 238
Bse1I ACTGG 2 cut(s) 175, 324
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 329
BseLI CCNNNNNNNGG 3 cut(s) 190, 226, 238
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 175, 324
BseXI GCAGC 1 cut(s) 143
BseYI CCCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 226
BsiSI CCGG 1 cut(s) 226
BslFI GGGAC 2 cut(s) 123, 188
BslI CCNNNNNNNGG 3 cut(s) 190, 226, 238
BsmFI GGGAC 2 cut(s) 123, 188
Bsp1286I GDGCHC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 5 cut(s) 33, 149, 194, 220, 255
BspCNI CTCAG 1 cut(s) 115
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 230
BsrI ACTGG 2 cut(s) 175, 324
BssECI CCNNGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 177
BstDEI CTNAG 2 cut(s) 123, 258
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 329
BstFNI CGCG 1 cut(s) 222
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 5 cut(s) 41, 128, 177, 186, 268
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 143
BstV2I GAAGAC 1 cut(s) 251
BstXI CCANNNNNNTGG 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 329
BtsIMutI CAGTG 3 cut(s) 182, 317, 334
Cfr13I GGNCC 2 cut(s) 196, 229
Csp6I GTAC 1 cut(s) 301
CviAII CATG 3 cut(s) 190, 211, 265
CviJI RGCY 8 cut(s) 44, 86, 131, 141, 156, 219, 262, 316
CviKI_1 RGCY 8 cut(s) 44, 86, 131, 141, 156, 219, 262, 316
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 2 cut(s) 123, 258
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 196, 229
Eco91I GGTNACC 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 3 cut(s) 193, 214, 268
FaiI YATR 3 cut(s) 191, 212, 266
FaqI GGGAC 2 cut(s) 123, 188
FatI CATG 3 cut(s) 189, 210, 264
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 157, 220
FokI GGATG 1 cut(s) 336
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 2 cut(s) 157, 220
GluI GCNGC 2 cut(s) 157, 220
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 66
HapII CCGG 1 cut(s) 226
Hin1II CATG 3 cut(s) 193, 214, 268
HpaII CCGG 1 cut(s) 226
HphI GGTGA 2 cut(s) 22, 353
Hpy188I TCNGA 1 cut(s) 233
Hpy188III TCNNGA 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4V TGCA 2 cut(s) 171, 189
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 128, 177, 186, 268
HpyF3I CTNAG 2 cut(s) 123, 258
Hsp92II CATG 3 cut(s) 193, 214, 268
LpnPI CCDG 9 cut(s) 52, 96, 145, 188, 229, 239, 275, 283, 305
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 341
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 2 cut(s) 4, 307
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 1 cut(s) 97
MslI CAYNNNNRTG 2 cut(s) 322, 339
MspI CCGG 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 5 cut(s) 41, 128, 177, 186, 268
NciI CCSGG 1 cut(s) 227
NlaIII CATG 3 cut(s) 193, 214, 268
NlaIV GGNNCC 1 cut(s) 230
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 341
OliI CACNNNNGTG 1 cut(s) 339
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 158, 221
PspEI GGTNACC 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 230
PspPI GGNCC 2 cut(s) 196, 229
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 2 cut(s) 322, 339
SatI GCNGC 2 cut(s) 157, 220
Sau96I GGNCC 2 cut(s) 196, 229
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 143
SetI ASST 2 cut(s) 158, 302
SfaNI GCATC 1 cut(s) 258
SinI GGWCC 2 cut(s) 196, 229
SmiMI CAYNNNNRTG 2 cut(s) 322, 339
Sse9I AATT 2 cut(s) 4, 307
SsiI CCGC 5 cut(s) 33, 149, 194, 220, 255
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 338
TasI AATT 2 cut(s) 4, 307
TauI GCSGC 1 cut(s) 222
TscAI CASTG 3 cut(s) 182, 324, 341
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 182, 324, 341
Tth111I GACNNNGTC 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 196, 229
XapI RAATTY 1 cut(s) 4
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.