MD15G1322000.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013513
Physical Location & Seq
Forward (+)
33883808 .. 33884701
894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1322000.v1.1.491

Sequence Viewer

Length: 384 bp
ATGAAAAACTCATTTTATCTTCATTTGAAAGCCACCAGAGCCATTGTCCTTGTGCATTTTCTTCTCTCTTCAATTCCAGCCTTTTGTTTTACTGCTCATCTGCCTCAGCCTCTCACTCCTCCTCTTCGGGGCATGTTATTTGAGGAGAAAACCAGGCTGGGTTCAGAACCACCAAGCTGCCACAACAAGTGCAATTATTGCCACCCATGCGTGGCGGTTCAAGTGCCCACCATTTCCAGCCGCGAGCGGCGGGTCGAACCAGGCATGACCCGAACCATCCCCATGATGTTCCTTGACCCATCTCATCCAGGTACCAACAAGTACTCGAATTACAAGCCTCTGGGTTGGAAATGTCACTGTGGCGATCACTTCTTCAACCCCTAG

Protein Analysis

128

Amino Acids

14.47

Weight (kDa)

9.23

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 52 - 127 1.7e-18 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 247
AccII CGCG 1 cut(s) 243
AciI CCGC 4 cut(s) 215, 241, 247, 250
AfaI GTAC 2 cut(s) 313, 323
AfiI CCNNNNNNNGG 2 cut(s) 128, 211
AgsI TTSAA 4 cut(s) 28, 72, 221, 376
AjnI CCWGG 3 cut(s) 152, 259, 307
AluBI AGCT 1 cut(s) 177
AluI AGCT 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 177
Asp718I GGTACC 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 228
BanI GGYRCC 1 cut(s) 311
BbvCI CCTCAGC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 164
BccI CCATC 2 cut(s) 284, 307
BciT130I CCWGG 3 cut(s) 154, 261, 309
BfaI CTAG 1 cut(s) 382
BisI GCNGC 3 cut(s) 178, 241, 248
BlsI GCNGC 3 cut(s) 179, 242, 249
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 154, 261, 309
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 3 cut(s) 154, 261, 309
Bpu10I CCTNAGC 1 cut(s) 105
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 211
BseBI CCWGG 3 cut(s) 154, 261, 309
BseGI GGATG 2 cut(s) 276, 304
BseLI CCNNNNNNNGG 2 cut(s) 128, 211
BseMII CTCAG 1 cut(s) 119
BseRI GAGGAG 3 cut(s) 108, 111, 158
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 1 cut(s) 164
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 128, 211
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 4 cut(s) 215, 241, 247, 250
BspCNI CTCAG 1 cut(s) 118
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 247
BssMI GATC 1 cut(s) 364
Bst2UI CCWGG 3 cut(s) 154, 261, 309
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 2 cut(s) 73, 129
BstAPI GCANNNNNTGC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 245
BstDEI CTNAG 1 cut(s) 105
BstF5I GGATG 2 cut(s) 276, 304
BstFNI CGCG 1 cut(s) 243
BstKTI GATC 1 cut(s) 367
BstMBI GATC 1 cut(s) 364
BstMWI GCNNNNNNNGC 3 cut(s) 38, 198, 207
BstNI CCWGG 3 cut(s) 154, 261, 309
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 3 cut(s) 152, 259, 307
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 164
BtsCI GGATG 2 cut(s) 276, 304
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 245
Csp6I GTAC 2 cut(s) 312, 322
CviAII CATG 4 cut(s) 133, 207, 265, 283
CviJI RGCY 8 cut(s) 32, 41, 80, 109, 157, 177, 240, 337
CviKI_1 RGCY 8 cut(s) 32, 41, 80, 109, 157, 177, 240, 337
CviQI GTAC 2 cut(s) 312, 322
DdeI CTNAG 1 cut(s) 105
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 73, 129
EarI CTCTTC 2 cut(s) 73, 129
EcoRII CCWGG 3 cut(s) 152, 259, 307
FaeI CATG 4 cut(s) 136, 210, 268, 286
FaiI YATR 4 cut(s) 134, 208, 266, 284
FatI CATG 4 cut(s) 132, 206, 264, 282
FauI CCCGC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 178, 241, 248
FokI GGATG 2 cut(s) 263, 291
Fsp4HI GCNGC 3 cut(s) 178, 241, 248
FspBI CTAG 1 cut(s) 382
GluI GCNGC 3 cut(s) 178, 241, 248
GsaI CCCAGC 1 cut(s) 161
Hin1II CATG 4 cut(s) 136, 210, 268, 286
Hpy188I TCNGA 1 cut(s) 166
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 2 cut(s) 55, 192
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 198, 207
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 4 cut(s) 136, 210, 268, 286
KpnI GGTACC 1 cut(s) 315
Kzo9I GATC 1 cut(s) 364
Lsp1109I GCAGC 1 cut(s) 164
MaeI CTAG 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 353
MalI GATC 1 cut(s) 366
MbiI CCGCTC 1 cut(s) 247
MboI GATC 1 cut(s) 364
MboII GAAGA 5 cut(s) 11, 53, 60, 116, 364
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 3 cut(s) 72, 193, 328
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 6 cut(s) 114, 120, 129, 132, 136, 348
MslI CAYNNNNRTG 1 cut(s) 281
MspR9I CCNGG 3 cut(s) 154, 261, 309
MvaI CCWGG 3 cut(s) 154, 261, 309
MvnI CGCG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 38, 198, 207
NdeII GATC 1 cut(s) 364
NlaIII CATG 4 cut(s) 136, 210, 268, 286
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 136
PkrI GCNGC 3 cut(s) 179, 242, 249
Psp6I CCWGG 3 cut(s) 152, 259, 307
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 3 cut(s) 152, 259, 307
PspN4I GGNNCC 1 cut(s) 313
RsaI GTAC 2 cut(s) 313, 323
RsaNI GTAC 2 cut(s) 312, 322
RseI CAYNNNNRTG 1 cut(s) 281
SatI GCNGC 3 cut(s) 178, 241, 248
Sau3AI GATC 1 cut(s) 364
ScaI AGTACT 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 154, 261, 309
SduI GDGCHC 1 cut(s) 228
SetI ASST 2 cut(s) 179, 313
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 3 cut(s) 72, 193, 328
SsiI CCGC 4 cut(s) 215, 241, 247, 250
SspMI CTAG 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 152, 259, 307
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 2 cut(s) 255, 326
TasI AATT 3 cut(s) 72, 193, 328
TatI WGTACW 1 cut(s) 321
TauI GCSGC 2 cut(s) 243, 250
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 2 cut(s) 11, 17
TspRI CASTG 1 cut(s) 362
XceI RCATGY 1 cut(s) 136
XspI CTAG 1 cut(s) 382
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.