pycom01g06080

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013513
Physical Location & Seq
Reverse (-)
6175559 .. 6176174
616 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g06080.1

Sequence Viewer

Length: 375 bp
ATGAATAATTACTCCCTTTATCTTCTTTTCAAAGTCCCCAGAGCCATCATGCTTCTGCATTTTCTTATCTCTTCAATTCCAGCCGCCTCTTGTTTTACCACTCATCTGCCTTCTCTTCGGGGAATGTTATTTGAGGAGAAAACCAGGCTAGGATCCAAACCACCAAGCTGCCACAACAAGTGCAACGAGTGCCACCCATGCATGGCGGTTCAAGTGCCCACAATGCCCAGCCACGACCGGCAGATCGAACCGGGCATGACCCGAACCATGTCCATGATGTTCTTTGACCCATCTCATCCAGGTACCAACAAGTACTCCAATTACAAGCCTCTGGGTTGGAAATGCCACTGTGGTGACAACTTCTTCAACCCCTAG

Protein Analysis

125

Amino Acids

14.16

Weight (kDa)

8.92

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 49 - 124 2.1e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 302
AciI CCGC 2 cut(s) 84, 206
AclWI GGATC 2 cut(s) 147, 160
AfaI GTAC 2 cut(s) 304, 314
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 4 cut(s) 31, 75, 212, 367
AjnI CCWGG 2 cut(s) 143, 298
AleI CACNNNNGTG 1 cut(s) 351
AluBI AGCT 1 cut(s) 168
AluI AGCT 1 cut(s) 168
AlwI GGATC 2 cut(s) 147, 160
ApeKI GCWGC 1 cut(s) 168
Asp718I GGTACC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 219
BamHI GGATCC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 302
BbvI GCAGC 1 cut(s) 155
BccI CCATC 2 cut(s) 53, 298
BciT130I CCWGG 2 cut(s) 145, 300
BcnI CCSGG 1 cut(s) 252
BfaI CTAG 2 cut(s) 149, 373
BisI GCNGC 2 cut(s) 84, 169
BlsI GCNGC 2 cut(s) 85, 170
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 3 cut(s) 145, 252, 300
BmiI GGNNCC 2 cut(s) 154, 304
BmrFI CCNGG 3 cut(s) 145, 252, 300
BpuMI CCSGG 1 cut(s) 252
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 237
BseBI CCWGG 2 cut(s) 145, 300
BseGI GGATG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 1 cut(s) 155
BseYI CCCAGC 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 238
BshNI GGYRCC 1 cut(s) 302
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 2 cut(s) 238, 251
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 2 cut(s) 152, 243
BspACI CCGC 2 cut(s) 84, 206
BspLI GGNNCC 2 cut(s) 154, 304
BspPI GGATC 2 cut(s) 147, 160
BspT107I GGYRCC 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 237
BssAI RCCGGY 1 cut(s) 237
BssMI GATC 2 cut(s) 152, 243
Bst2UI CCWGG 2 cut(s) 145, 300
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 2 cut(s) 76, 120
BstAPI GCANNNNNTGC 1 cut(s) 189
BstF5I GGATG 1 cut(s) 295
BstKTI GATC 2 cut(s) 155, 246
BstMBI GATC 2 cut(s) 152, 243
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 189, 198, 223
BstNI CCWGG 2 cut(s) 145, 300
BstSCI CCNGG 3 cut(s) 143, 250, 298
BstSLI GKGCMC 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BtsCI GGATG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 237
Csp6I GTAC 2 cut(s) 303, 313
CviAII CATG 6 cut(s) 49, 198, 202, 256, 268, 274
CviJI RGCY 6 cut(s) 44, 83, 148, 168, 231, 328
CviKI_1 RGCY 6 cut(s) 44, 83, 148, 168, 231, 328
CviQI GTAC 2 cut(s) 303, 313
DpnI GATC 2 cut(s) 154, 245
DpnII GATC 2 cut(s) 152, 243
Eam1104I CTCTTC 2 cut(s) 76, 120
EarI CTCTTC 2 cut(s) 76, 120
EcoRII CCWGG 2 cut(s) 143, 298
EcoT22I ATGCAT 1 cut(s) 203
FaeI CATG 6 cut(s) 52, 201, 205, 259, 271, 277
FaiI YATR 6 cut(s) 50, 199, 203, 257, 269, 275
FaqI GGGAC 1 cut(s) 20
FatI CATG 6 cut(s) 48, 197, 201, 255, 267, 273
Fnu4HI GCNGC 2 cut(s) 84, 169
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 84, 169
FspBI CTAG 2 cut(s) 149, 373
GluI GCNGC 2 cut(s) 84, 169
GsaI CCCAGC 1 cut(s) 231
HapII CCGG 2 cut(s) 238, 251
Hin1II CATG 6 cut(s) 52, 201, 205, 259, 271, 277
HpaII CCGG 2 cut(s) 238, 251
HphI GGTGA 1 cut(s) 365
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 3 cut(s) 58, 183, 201
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 198, 223
Hsp92II CATG 6 cut(s) 52, 201, 205, 259, 271, 277
KpnI GGTACC 1 cut(s) 306
Kzo9I GATC 2 cut(s) 152, 243
Lsp1109I GCAGC 1 cut(s) 155
MaeI CTAG 2 cut(s) 149, 373
MaeIII GTNAC 1 cut(s) 353
MalI GATC 2 cut(s) 154, 245
MboI GATC 2 cut(s) 152, 243
MboII GAAGA 4 cut(s) 14, 63, 107, 355
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 3 cut(s) 7, 75, 319
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 3 cut(s) 97, 127, 339
Mph1103I ATGCAT 1 cut(s) 203
MslI CAYNNNNRTG 2 cut(s) 272, 351
MspI CCGG 2 cut(s) 238, 251
MspR9I CCNGG 3 cut(s) 145, 252, 300
MvaI CCWGG 2 cut(s) 145, 300
MwoI GCNNNNNNNGC 3 cut(s) 189, 198, 223
NciI CCSGG 1 cut(s) 252
NdeII GATC 2 cut(s) 152, 243
NlaIII CATG 6 cut(s) 52, 201, 205, 259, 271, 277
NlaIV GGNNCC 2 cut(s) 154, 304
NmuCI GTSAC 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 203
OliI CACNNNNGTG 1 cut(s) 351
PkrI GCNGC 2 cut(s) 85, 170
Psp6I CCWGG 2 cut(s) 143, 298
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 143, 298
PspN4I GGNNCC 2 cut(s) 154, 304
PsuI RGATCY 1 cut(s) 152
RsaI GTAC 2 cut(s) 304, 314
RsaNI GTAC 2 cut(s) 303, 313
RseI CAYNNNNRTG 2 cut(s) 272, 351
SatI GCNGC 2 cut(s) 84, 169
Sau3AI GATC 2 cut(s) 152, 243
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 145, 252, 300
SduI GDGCHC 1 cut(s) 219
SetI ASST 2 cut(s) 170, 304
SmiMI CAYNNNNRTG 2 cut(s) 272, 351
Sse9I AATT 3 cut(s) 7, 75, 319
SsiI CCGC 2 cut(s) 84, 206
SspMI CTAG 2 cut(s) 149, 373
StyD4I CCNGG 3 cut(s) 143, 250, 298
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 246
TasI AATT 3 cut(s) 7, 75, 319
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 86
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 168
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 353
XspI CTAG 2 cut(s) 149, 373
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.