Rw2G018350

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013513
Physical Location & Seq
Forward (+)
23512926 .. 23513379
454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018350.1

Sequence Viewer

Length: 357 bp
ATGAATTCTTCAATAAATCTTCGGTTTTTCACCGCAACCAGAGCCATTATTGTTCTTCTTCTTCTTGTTCTCTCTCCAGCCTCTTGTTTTACTTCTCTTCGGGGACTATTGTTTGCTGAGAAAGCCAGATTGGGCTCAACACCACCGAGCTGCCACAACAAGTGCAACCAGTGCCACCCTTGCATGGCGGTCCAAGTCCCCACCATGCCCAGCCGCGACAGGGTCCGACCCGTGGGGAAGACCCGACCCGCTAAGCCCATGATGCTCTTTGACCCGTCTCATCTGGAAAACAGGTACTCCAATTACAAGCCACTGGGTTGGAAATGCCATTGTGGTGACCACTTCTTCAATCCCTAG

Protein Analysis

118

Amino Acids

13.29

Weight (kDa)

9.77

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 43 - 118 1.3e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 216
AciI CCGC 4 cut(s) 33, 188, 214, 249
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 3 cut(s) 184, 220, 232
AgsI TTSAA 2 cut(s) 12, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 282
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 4
AspS9I GGNCC 2 cut(s) 190, 223
AsuHPI GGTGA 2 cut(s) 22, 347
AvaII GGWCC 2 cut(s) 190, 223
BanII GRGCYC 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 282
BfaI CTAG 1 cut(s) 355
BisI GCNGC 2 cut(s) 151, 214
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 2 cut(s) 152, 215
Bme18I GGWCC 2 cut(s) 190, 223
BmgT120I GGNCC 2 cut(s) 190, 223
BmiI GGNNCC 1 cut(s) 224
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 220, 232
Bse1I ACTGG 2 cut(s) 169, 318
BseDI CCNNGG 1 cut(s) 231
BseLI CCNNNNNNNGG 3 cut(s) 184, 220, 232
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 2 cut(s) 169, 318
BseXI GCAGC 1 cut(s) 137
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 216
BslFI GGGAC 2 cut(s) 117, 182
BslI CCNNNNNNNGG 3 cut(s) 184, 220, 232
BsmAI GTCTC 1 cut(s) 282
BsmBI CGTCTC 1 cut(s) 282
BsmFI GGGAC 2 cut(s) 117, 182
Bsp1286I GDGCHC 1 cut(s) 137
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 4 cut(s) 33, 188, 214, 249
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 224
BsrI ACTGG 2 cut(s) 169, 318
BssECI CCNNGG 1 cut(s) 231
Bst6I CTCTTC 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 117, 252
BstDSI CCRYGG 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 335
BstFNI CGCG 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 41, 122, 171, 180, 262
BstPI GGTNACC 1 cut(s) 335
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 137
BstV2I GAAGAC 1 cut(s) 245
BstXI CCANNNNNNTGG 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 176, 311
Cfr13I GGNCC 2 cut(s) 190, 223
Csp6I GTAC 1 cut(s) 295
CviAII CATG 3 cut(s) 184, 205, 259
CviJI RGCY 8 cut(s) 44, 80, 125, 135, 150, 213, 256, 310
CviKI_1 RGCY 8 cut(s) 44, 80, 125, 135, 150, 213, 256, 310
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 2 cut(s) 117, 252
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 190, 223
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 137
Esp3I CGTCTC 1 cut(s) 282
FaeI CATG 3 cut(s) 187, 208, 262
FaiI YATR 3 cut(s) 185, 206, 260
FaqI GGGAC 2 cut(s) 117, 182
FatI CATG 3 cut(s) 183, 204, 258
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 2 cut(s) 151, 214
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 151, 214
FspBI CTAG 1 cut(s) 355
GluI GCNGC 2 cut(s) 151, 214
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 60
Hin1II CATG 3 cut(s) 187, 208, 262
HphI GGTGA 2 cut(s) 22, 347
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 1 cut(s) 284
HpyCH4V TGCA 2 cut(s) 165, 183
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 122, 171, 180, 262
HpyF3I CTNAG 2 cut(s) 117, 252
Hsp92II CATG 3 cut(s) 187, 208, 262
LpnPI CCDG 9 cut(s) 52, 90, 139, 182, 205, 223, 269, 277, 299
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 355
MaeIII GTNAC 1 cut(s) 335
MboII GAAGA 7 cut(s) 11, 47, 50, 53, 89, 250, 337
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 2 cut(s) 4, 301
MmeI TCCRAC 2 cut(s) 250, 299
MnlI CCTC 1 cut(s) 91
MslI CAYNNNNRTG 1 cut(s) 333
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 5 cut(s) 41, 122, 171, 180, 262
NlaIII CATG 3 cut(s) 187, 208, 262
NlaIV GGNNCC 1 cut(s) 224
NmuCI GTSAC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 152, 215
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 2 cut(s) 190, 223
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 333
SatI GCNGC 2 cut(s) 151, 214
Sau96I GGNCC 2 cut(s) 190, 223
SduI GDGCHC 1 cut(s) 137
SetI ASST 2 cut(s) 152, 296
SfaNI GCATC 1 cut(s) 252
SinI GGWCC 2 cut(s) 190, 223
SmiMI CAYNNNNRTG 1 cut(s) 333
Sse9I AATT 2 cut(s) 4, 301
SsiI CCGC 4 cut(s) 33, 188, 214, 249
SspMI CTAG 1 cut(s) 355
TasI AATT 2 cut(s) 4, 301
TauI GCSGC 1 cut(s) 216
TscAI CASTG 2 cut(s) 176, 318
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 176, 318
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 190, 223
XapI RAATTY 1 cut(s) 4
XspI CTAG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.