FvH4_1g20610

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015316
Physical Location & Seq
Reverse (-)
12789860 .. 12793280
3421 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20610.t1

Sequence Viewer

Length: 951 bp
ATGCCGATGTGCTTCAGCTTGATCGCCTCGCGCGACTCCTGCTTCCGCTCCTCCTTCTCCTCCGCCGGCCTCAAATCCACCACCACCGATCTCGGCGAGGGCACCATCATCCACAGCTGGGTCCCCAAACACCCCTCCCCATCCAAACCTACCCTCCTCCTCATCCACGGCATCGGCGCCAACGCAATGTGGCAGTGGCACGAGTTCATCACCCCTCTCACTTCCCGCTTCAACCTCTACGTCCCCGACCTCATCTTCTTCGGGGACTCCTACTCATCCCGGAGCGAACGCTCCGAGACGTTCCAGGCCCAGTGCATCATGGGACTCATGGAGGCCCATGACGTCAAGGGGAAAATGCACGTGGCCGGGATCAGCTACGGCGGGTTCGTGGCTTACAGCATGGCTGCGCAGTATGGGGAGAGGATTGAGAGGTTGGTGTTGTGCTGCGCCGGAATCTGCGCCGAGGAGAAGGACATGGAGAAGGGGATGTTTCAGGTGAAGAGTGTGGAGGAGGCGGTGAGTATATTGCTGCCGCAGAGGGCGGCGCAGGTGAGGCAGTTGATGAAGATCTCGTTTTACAAGCCGGCGACCAATGTGCCCTCTTGTTTTCTCAACGATTTTATTGAGGTAATGTGCACAGATCACGTTCAAGAGAGGAGGGAACTTATTGAAGCATTACATAAGGACAGAAAGATGTCTGATCTTCCAAAGATAACTCATCCTACACTACTAATCTGGGGGGAGCATGACCTGGTATTCCCAATGGAATTGGCACATCGATTGAAAAGGCAAATAGGCGAGAGTGCAGAACTAGTGACCATAAAGAATGCAGGACATGCTATAAATGTAGAGAAACCGAAAGAGATGCAGAAGTACATGAAGTCATTCCTCCTTGATCCACTCCCTCAACCTAAACATGAAAAACATAGCAATGGCCACAAGGTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

317

Amino Acids

35.72

Weight (kDa)

7.24

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 49 - 285 2.9e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 50 - 286 1.7e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 288 5.9e-16 Alpha/beta hydrolase family
Abhydrolase_4 PF08386 237 - 300 2e-06 TAP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 538
AatII GACGTC 1 cut(s) 345
Acc16I TGCGCA 1 cut(s) 408
Acc36I ACCTGC 1 cut(s) 538
AccB1I GGYRCC 2 cut(s) 101, 176
AccB7I CCANNNNNTGG 1 cut(s) 943
AccBSI CCGCTC 1 cut(s) 48
AccII CGCG 2 cut(s) 31, 33
AciI CCGC 7 cut(s) 46, 63, 226, 381, 515, 533, 542
AclWI GGATC 2 cut(s) 377, 890
AcoI YGGCCR 2 cut(s) 363, 934
AcvI CACGTG 1 cut(s) 361
AcyI GRCGYC 2 cut(s) 177, 342
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 2 cut(s) 118, 943
AgsI TTSAA 4 cut(s) 232, 650, 671, 784
AhlI ACTAGT 1 cut(s) 811
AjnI CCWGG 2 cut(s) 303, 750
AluBI AGCT 3 cut(s) 18, 117, 375
AluI AGCT 3 cut(s) 18, 117, 375
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 1 cut(s) 290
Alw44I GTGCAC 1 cut(s) 634
AlwI GGATC 2 cut(s) 377, 890
AoxI GGCC 5 cut(s) 67, 306, 333, 363, 934
ApaLI GTGCAC 1 cut(s) 634
ApeKI GCWGC 3 cut(s) 404, 444, 529
Asp700I GAANNNNTTC 1 cut(s) 884
AspLEI GCGC 6 cut(s) 33, 179, 409, 449, 461, 547
AspS9I GGNCC 3 cut(s) 121, 307, 334
AsuC2I CCSGG 2 cut(s) 280, 367
AsuHPI GGTGA 4 cut(s) 202, 508, 529, 562
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 3 cut(s) 104, 600, 638
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 2 cut(s) 101, 176
BauI CACGAG 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 3 cut(s) 391, 431, 516
BccI CCATC 2 cut(s) 113, 148
BceAI ACGGC 2 cut(s) 184, 394
BcgI CGANNNNNNTGC 4 cut(s) 577, 611, 847, 881
BciT130I CCWGG 2 cut(s) 305, 752
BcnI CCSGG 2 cut(s) 280, 367
BcoDI GTCTC 1 cut(s) 290
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 1 cut(s) 812
BfoI RGCGCY 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 538
BglII AGATCT 1 cut(s) 567
BisI GCNGC 5 cut(s) 405, 445, 530, 533, 543
BlsI GCNGC 5 cut(s) 406, 446, 531, 534, 544
Bme1390I CCNGG 4 cut(s) 280, 305, 367, 752
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 3 cut(s) 121, 307, 334
BmiI GGNNCC 4 cut(s) 103, 122, 123, 178
BmrFI CCNGG 4 cut(s) 280, 305, 367, 752
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 180, 324, 855
BmuI ACTGGG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 280, 367
Bsa29I ATCGAT 1 cut(s) 778
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 2 cut(s) 177, 342
BsaJI CCNNGG 2 cut(s) 166, 462
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 943
Bse118I RCCGGY 2 cut(s) 65, 583
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 2 cut(s) 192, 937
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 2 cut(s) 305, 752
BseCI ATCGAT 1 cut(s) 778
BseDI CCNNGG 2 cut(s) 166, 462
BseGI GGATG 6 cut(s) 108, 140, 162, 275, 492, 718
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 2 cut(s) 118, 943
BseMI GCAATG 2 cut(s) 192, 937
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 7 cut(s) 40, 49, 146, 149, 479, 524, 670
BseSI GKGCMC 3 cut(s) 104, 600, 638
BseXI GCAGC 3 cut(s) 391, 431, 516
BseYI CCCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 31, 33
BshFI GGCC 5 cut(s) 69, 308, 335, 365, 936
BshNI GGYRCC 2 cut(s) 101, 176
BshVI ATCGAT 1 cut(s) 778
BsiHKAI GWGCWC 1 cut(s) 638
BsiSI CCGG 5 cut(s) 66, 280, 366, 450, 584
BslFI GGGAC 4 cut(s) 107, 227, 278, 336
BslI CCNNNNNNNGG 2 cut(s) 118, 943
BsmAI GTCTC 1 cut(s) 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 4 cut(s) 107, 227, 278, 336
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 5 cut(s) 69, 308, 335, 365, 936
Bsp1286I GDGCHC 3 cut(s) 104, 600, 638
Bsp143I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BspACI CCGC 7 cut(s) 46, 63, 226, 381, 515, 533, 542
BspANI GGCC 5 cut(s) 69, 308, 335, 365, 936
BspDI ATCGAT 1 cut(s) 778
BspFNI CGCG 2 cut(s) 31, 33
BspLI GGNNCC 4 cut(s) 103, 122, 123, 178
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 2 cut(s) 377, 890
BspT107I GGYRCC 2 cut(s) 101, 176
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 192, 937
BsrFI RCCGGY 2 cut(s) 65, 583
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 65, 583
BssECI CCNNGG 2 cut(s) 166, 462
BssMI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BssNI GRCGYC 2 cut(s) 177, 342
BssSI CACGAG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 305, 752
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 2 cut(s) 177, 342
BstAPI GCANNNNNTGC 1 cut(s) 836
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 67, 585
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 6 cut(s) 108, 140, 162, 275, 492, 718
BstFNI CGCG 2 cut(s) 31, 33
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 6 cut(s) 33, 179, 409, 449, 461, 547
BstKTI GATC 7 cut(s) 24, 91, 372, 570, 643, 703, 898
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BstMWI GCNNNNNNNGC 3 cut(s) 39, 553, 836
BstNI CCWGG 2 cut(s) 305, 752
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 4 cut(s) 278, 303, 365, 750
BstSLI GKGCMC 3 cut(s) 104, 600, 638
BstUI CGCG 2 cut(s) 31, 33
BstV1I GCAGC 3 cut(s) 391, 431, 516
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 778
BsuRI GGCC 5 cut(s) 69, 308, 335, 365, 936
BsuTUI ATCGAT 1 cut(s) 778
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 6 cut(s) 108, 140, 162, 275, 492, 718
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 317
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 67, 585
CfoI GCGC 6 cut(s) 33, 179, 409, 449, 461, 547
Cfr10I RCCGGY 2 cut(s) 65, 583
Cfr13I GGNCC 3 cut(s) 121, 307, 334
ClaI ATCGAT 1 cut(s) 778
CsiI ACCWGGT 1 cut(s) 750
Csp6I GTAC 1 cut(s) 874
CviAII CATG 9 cut(s) 319, 328, 338, 400, 475, 746, 836, 877, 917
CviQI GTAC 1 cut(s) 874
DinI GGCGCC 1 cut(s) 178
DpnI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
DpnII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
DraIII CACNNNGTG 1 cut(s) 943
EaeI YGGCCR 2 cut(s) 363, 934
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 121
Eco72I CACGTG 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 303, 750
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 9 cut(s) 322, 331, 341, 403, 478, 749, 839, 880, 920
FaqI GGGAC 4 cut(s) 107, 227, 278, 336
FatI CATG 9 cut(s) 318, 327, 337, 399, 474, 745, 835, 876, 916
FauI CCCGC 2 cut(s) 233, 374
Fnu4HI GCNGC 5 cut(s) 405, 445, 530, 533, 543
FokI GGATG 6 cut(s) 95, 127, 149, 262, 499, 705
Fsp4HI GCNGC 5 cut(s) 405, 445, 530, 533, 543
FspBI CTAG 1 cut(s) 812
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 6 cut(s) 32, 178, 408, 448, 460, 546
GluI GCNGC 5 cut(s) 405, 445, 530, 533, 543
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 5 cut(s) 69, 308, 335, 365, 936
HapII CCGG 5 cut(s) 66, 280, 366, 450, 584
HhaI GCGC 6 cut(s) 33, 179, 409, 449, 461, 547
Hin1I GRCGYC 2 cut(s) 177, 342
Hin1II CATG 9 cut(s) 322, 331, 341, 403, 478, 749, 839, 880, 920
Hin6I GCGC 6 cut(s) 31, 177, 407, 447, 459, 545
HinP1I GCGC 6 cut(s) 31, 177, 407, 447, 459, 545
HinfI GANTC 4 cut(s) 35, 266, 324, 453
HpaII CCGG 5 cut(s) 66, 280, 366, 450, 584
HphI GGTGA 4 cut(s) 202, 508, 529, 562
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 2 cut(s) 295, 700
Hpy188III TCNNGA 1 cut(s) 650
Hpy8I GTNNAC 1 cut(s) 636
HpyAV CCTTC 3 cut(s) 64, 463, 475
HpyCH4IV ACGT 5 cut(s) 240, 299, 342, 360, 645
HpyCH4V TGCA 6 cut(s) 315, 358, 636, 806, 830, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 553, 836
HpySE526I ACGT 5 cut(s) 240, 299, 342, 360, 645
Hsp92I GRCGYC 2 cut(s) 177, 342
Hsp92II CATG 9 cut(s) 322, 331, 341, 403, 478, 749, 839, 880, 920
HspAI GCGC 6 cut(s) 31, 177, 407, 447, 459, 545
KasI GGCGCC 1 cut(s) 176
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 2 cut(s) 65, 583
KroNI GCCGGC 2 cut(s) 67, 585
Kzo9I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
LmnI GCTCC 4 cut(s) 53, 282, 296, 742
Lsp1109I GCAGC 3 cut(s) 391, 431, 516
LweI GCATC 3 cut(s) 180, 324, 855
MabI ACCWGGT 1 cut(s) 750
MaeI CTAG 1 cut(s) 812
MaeII ACGT 5 cut(s) 240, 299, 342, 360, 645
MaeIII GTNAC 1 cut(s) 814
MalI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
MbiI CCGCTC 1 cut(s) 48
MboI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
MboII GAAGA 5 cut(s) 247, 250, 511, 577, 695
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 3 cut(s) 104, 600, 638
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 936
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 3 cut(s) 29, 260, 318
Mox20I TGGCCA 1 cut(s) 936
MroNI GCCGGC 2 cut(s) 65, 583
MroXI GAANNNNTTC 1 cut(s) 884
MscI TGGCCA 1 cut(s) 936
MseI TTAA 1 cut(s) 949
MslI CAYNNNNRTG 1 cut(s) 930
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 5 cut(s) 66, 280, 366, 450, 584
MspR9I CCNGG 4 cut(s) 280, 305, 367, 752
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 2 cut(s) 305, 752
MvnI CGCG 2 cut(s) 31, 33
MwoI GCNNNNNNNGC 3 cut(s) 39, 553, 836
NaeI GCCGGC 2 cut(s) 67, 585
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 280, 367
NdeII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
NgoMIV GCCGGC 2 cut(s) 65, 583
NlaIII CATG 9 cut(s) 322, 331, 341, 403, 478, 749, 839, 880, 920
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 178
NmeAIII GCCGAG 2 cut(s) 72, 487
NmuCI GTSAC 1 cut(s) 814
NsbI TGCGCA 1 cut(s) 408
NspI RCATGY 1 cut(s) 839
PaqCI CACCTGC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 384
PctI GAATGC 1 cut(s) 832
PdiI GCCGGC 2 cut(s) 67, 585
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 5 cut(s) 406, 446, 531, 534, 544
PleI GAGTC 3 cut(s) 29, 260, 318
PluTI GGCGCC 1 cut(s) 180
PmaCI CACGTG 1 cut(s) 361
PmlI CACGTG 1 cut(s) 361
PpsI GAGTC 3 cut(s) 29, 260, 318
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 303, 750
PspCI CACGTG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 303, 750
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 178
PspPI GGNCC 3 cut(s) 121, 307, 334
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 1 cut(s) 930
SaqAI TTAA 1 cut(s) 949
SatI GCNGC 5 cut(s) 405, 445, 530, 533, 543
Sau3AI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
Sau96I GGNCC 3 cut(s) 121, 307, 334
SchI GAGTC 3 cut(s) 29, 260, 318
ScrFI CCNGG 4 cut(s) 280, 305, 367, 752
SduI GDGCHC 3 cut(s) 104, 600, 638
SexAI ACCWGGT 1 cut(s) 750
SfaNI GCATC 3 cut(s) 180, 324, 855
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 930
SpeI ACTAGT 1 cut(s) 811
Sse9I AATT 1 cut(s) 767
SsiI CCGC 7 cut(s) 46, 63, 226, 381, 515, 533, 542
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 4 cut(s) 278, 303, 365, 750
TaiI ACGT 5 cut(s) 243, 302, 345, 363, 648
TaqI TCGA 1 cut(s) 778
TasI AATT 1 cut(s) 767
TatI WGTACW 1 cut(s) 873
TauI GCSGC 2 cut(s) 535, 545
TfiI GAWTC 1 cut(s) 453
Tru1I TTAA 1 cut(s) 949
Tru9I TTAA 1 cut(s) 949
TscAI CASTG 2 cut(s) 200, 317
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 3 cut(s) 404, 444, 529
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 4 cut(s) 196, 578, 893, 933
TspRI CASTG 2 cut(s) 200, 317
Van91I CCANNNNNTGG 1 cut(s) 943
VneI GTGCAC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 121
XceI RCATGY 1 cut(s) 839
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 1 cut(s) 812
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.