Rh2AG238600

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015316
Physical Location & Seq
Reverse (-)
26039697 .. 26042140
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG238600.1

Sequence Viewer

Length: 732 bp
ATGCCGATGTGCTTCAGCTTGATCGCCTCGCGCGACTCCTGCTTCCGCTACTCTTTCTCCTCCGCCGGCCTCAAGTCCACCACCACCGATCTCGGCGAAGGCACCATCATCCACAGTTGGGTCCCCAAACACCCCTCCCCATCCAAACCCACCCTGCTCCTCATACATGGCATCGGCGCCAACGCAATGTGGCAGTGGCACGAGTTCATCACCCCTCTCATTTCCCGCTTCAACCTCTACGTCCCCGACCTCATTTTCTTCGGGGACTCCTATTCGTCCAGGAGTGAACGCTCCGAGACTTTTCAGGCCCAGTGCGTCATGGGCCTCATGGAGGCCCATGGCGTCAGGGGAAAAATGCACGTAGCCGGAATCAGCTACGGGGGGTTCGTGGCCTATAGCATGGCGGCTCAGTATGGGGAGAGGATTGAGAGGTTGGTGTTGTGTTGCGCTGGAGTTTGTGCCGAGGAGAAGGATATGGAGAAGGGGATGTTTCAGGTGAAGAGTGTGGAGGAGGCAGTGAGTATATTGCTGCCGCAGAGGGCGGCACAGGTGAGGCAGTTGATGAAGATCTCGTTTTACAAGCCGGCGACCAATGTGCCCTCTTGTTTTCTCAACGATTTTATTGAGGTAATGTGCACAGATCACGTTCAAGAGAGGAGGGAACTTATTCAAGCATTACATAAGGACAGAAAAATGTCTGATCTTCCCAAGATAACTCATGTAATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

243

Amino Acids

27.38

Weight (kDa)

7.62

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 50 - 158 5.8e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 181 8.9e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 101, 176
AccII CGCG 2 cut(s) 31, 33
AciI CCGC 6 cut(s) 46, 63, 226, 404, 533, 542
AcyI GRCGYC 2 cut(s) 177, 342
AfiI CCNNNNNNNGG 2 cut(s) 118, 331
AgsI TTSAA 3 cut(s) 232, 650, 671
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 2 cut(s) 18, 375
AluI AGCT 2 cut(s) 18, 375
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 1 cut(s) 290
Alw44I GTGCAC 1 cut(s) 634
AoxI GGCC 5 cut(s) 67, 306, 322, 333, 390
ApaLI GTGCAC 1 cut(s) 634
ApeKI GCWGC 1 cut(s) 529
Asp700I GAANNNNTTC 1 cut(s) 666
AspLEI GCGC 3 cut(s) 33, 179, 449
AspS9I GGNCC 4 cut(s) 121, 307, 322, 334
AsuHPI GGTGA 3 cut(s) 202, 508, 562
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 2 cut(s) 600, 638
BanI GGYRCC 2 cut(s) 101, 176
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 516
BccI CCATC 2 cut(s) 113, 148
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 1 cut(s) 180
BglII AGATCT 1 cut(s) 567
BisI GCNGC 4 cut(s) 405, 530, 533, 543
BlsI GCNGC 4 cut(s) 406, 531, 534, 544
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 4 cut(s) 121, 307, 322, 334
BmiI GGNNCC 4 cut(s) 103, 122, 123, 178
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 2 cut(s) 177, 342
BsaJI CCNNGG 2 cut(s) 337, 462
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 331
Bse118I RCCGGY 2 cut(s) 65, 583
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 337, 462
BseGI GGATG 3 cut(s) 108, 140, 492
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 2 cut(s) 118, 331
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 5 cut(s) 49, 149, 479, 524, 670
BseSI GKGCMC 2 cut(s) 600, 638
BseXI GCAGC 1 cut(s) 516
Bsh1236I CGCG 2 cut(s) 31, 33
BshFI GGCC 5 cut(s) 69, 308, 324, 335, 392
BshNI GGYRCC 2 cut(s) 101, 176
BsiHKAI GWGCWC 1 cut(s) 638
BsiSI CCGG 3 cut(s) 66, 366, 584
BslFI GGGAC 3 cut(s) 107, 227, 278
BslI CCNNNNNNNGG 2 cut(s) 118, 331
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 3 cut(s) 107, 227, 278
BsnI GGCC 5 cut(s) 69, 308, 324, 335, 392
Bsp1286I GDGCHC 2 cut(s) 600, 638
Bsp143I GATC 5 cut(s) 21, 88, 567, 640, 700
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 6 cut(s) 46, 63, 226, 404, 533, 542
BspANI GGCC 5 cut(s) 69, 308, 324, 335, 392
BspCNI CTCAG 1 cut(s) 421
BspFNI CGCG 2 cut(s) 31, 33
BspLI GGNNCC 4 cut(s) 103, 122, 123, 178
BspT107I GGYRCC 2 cut(s) 101, 176
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 65, 583
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 65, 583
BssECI CCNNGG 2 cut(s) 337, 462
BssMI GATC 5 cut(s) 21, 88, 567, 640, 700
BssNI GRCGYC 2 cut(s) 177, 342
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 2 cut(s) 177, 342
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 67, 585
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 3 cut(s) 108, 140, 492
BstFNI CGCG 2 cut(s) 31, 33
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 3 cut(s) 33, 179, 449
BstKTI GATC 5 cut(s) 24, 91, 570, 643, 703
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 5 cut(s) 21, 88, 567, 640, 700
BstMWI GCNNNNNNNGC 2 cut(s) 39, 321
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 600, 638
BstUI CGCG 2 cut(s) 31, 33
BstV1I GCAGC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BsuRI GGCC 5 cut(s) 69, 308, 324, 335, 392
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 3 cut(s) 108, 140, 492
BtsI GCAGTG 2 cut(s) 200, 522
BtsIMutI CAGTG 3 cut(s) 200, 317, 522
Cac8I GCNNGC 2 cut(s) 67, 585
CfoI GCGC 3 cut(s) 33, 179, 449
Cfr10I RCCGGY 2 cut(s) 65, 583
Cfr13I GGNCC 4 cut(s) 121, 307, 322, 334
CseI GACGC 2 cut(s) 304, 331
CviAII CATG 6 cut(s) 167, 319, 328, 338, 400, 719
DdeI CTNAG 1 cut(s) 408
DinI GGCGCC 1 cut(s) 178
DpnI GATC 5 cut(s) 23, 90, 569, 642, 702
DpnII GATC 5 cut(s) 21, 88, 567, 640, 700
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 6 cut(s) 170, 322, 331, 341, 403, 722
FaqI GGGAC 3 cut(s) 107, 227, 278
FatI CATG 6 cut(s) 166, 318, 327, 337, 399, 718
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 4 cut(s) 405, 530, 533, 543
FokI GGATG 3 cut(s) 95, 127, 499
Fsp4HI GCNGC 4 cut(s) 405, 530, 533, 543
GlaI GCGC 3 cut(s) 32, 178, 448
GluI GCNGC 4 cut(s) 405, 530, 533, 543
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 5 cut(s) 69, 308, 324, 335, 392
HapII CCGG 3 cut(s) 66, 366, 584
HgaI GACGC 2 cut(s) 304, 331
HhaI GCGC 3 cut(s) 33, 179, 449
Hin1I GRCGYC 2 cut(s) 177, 342
Hin1II CATG 6 cut(s) 170, 322, 331, 341, 403, 722
Hin6I GCGC 3 cut(s) 31, 177, 447
HinP1I GCGC 3 cut(s) 31, 177, 447
HinfI GANTC 3 cut(s) 35, 266, 369
HpaII CCGG 3 cut(s) 66, 366, 584
HphI GGTGA 3 cut(s) 202, 508, 562
Hpy166II GTNNAC 3 cut(s) 78, 287, 636
Hpy188I TCNGA 2 cut(s) 295, 700
Hpy188III TCNNGA 1 cut(s) 650
Hpy8I GTNNAC 3 cut(s) 78, 287, 636
HpyAV CCTTC 3 cut(s) 92, 463, 475
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 3 cut(s) 240, 360, 645
HpyCH4V TGCA 2 cut(s) 358, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 321
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 3 cut(s) 240, 360, 645
Hsp92I GRCGYC 2 cut(s) 177, 342
Hsp92II CATG 6 cut(s) 170, 322, 331, 341, 403, 722
HspAI GCGC 3 cut(s) 31, 177, 447
KasI GGCGCC 1 cut(s) 176
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 2 cut(s) 65, 583
KroNI GCCGGC 2 cut(s) 67, 585
Kzo9I GATC 5 cut(s) 21, 88, 567, 640, 700
LmnI GCTCC 2 cut(s) 162, 296
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 1 cut(s) 180
MaeII ACGT 3 cut(s) 240, 360, 645
MalI GATC 5 cut(s) 23, 90, 569, 642, 702
MboI GATC 5 cut(s) 21, 88, 567, 640, 700
MboII GAAGA 4 cut(s) 250, 511, 577, 695
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 600, 638
MluCI AATT 1 cut(s) 723
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 2 cut(s) 29, 260
MroNI GCCGGC 2 cut(s) 65, 583
MroXI GAANNNNTTC 1 cut(s) 666
MspI CCGG 3 cut(s) 66, 366, 584
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 2 cut(s) 31, 33
MwoI GCNNNNNNNGC 2 cut(s) 39, 321
NaeI GCCGGC 2 cut(s) 67, 585
NarI GGCGCC 1 cut(s) 177
NcoI CCATGG 1 cut(s) 337
NdeII GATC 5 cut(s) 21, 88, 567, 640, 700
NgoMIV GCCGGC 2 cut(s) 65, 583
NlaIII CATG 6 cut(s) 170, 322, 331, 341, 403, 722
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 178
NmeAIII GCCGAG 2 cut(s) 72, 487
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 384
PdiI GCCGGC 2 cut(s) 67, 585
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 1 cut(s) 369
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 4 cut(s) 406, 531, 534, 544
PleI GAGTC 2 cut(s) 29, 260
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 2 cut(s) 29, 260
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 178
PspPI GGNCC 4 cut(s) 121, 307, 322, 334
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 567
SatI GCNGC 4 cut(s) 405, 530, 533, 543
Sau3AI GATC 5 cut(s) 21, 88, 567, 640, 700
Sau96I GGNCC 4 cut(s) 121, 307, 322, 334
SchI GAGTC 2 cut(s) 29, 260
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 600, 638
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 1 cut(s) 394
SfoI GGCGCC 1 cut(s) 178
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 1 cut(s) 723
SsiI CCGC 6 cut(s) 46, 63, 226, 404, 533, 542
SspDI GGCGCC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 3 cut(s) 243, 363, 648
TasI AATT 1 cut(s) 723
TauI GCSGC 3 cut(s) 407, 535, 545
TfiI GAWTC 1 cut(s) 369
TscAI CASTG 3 cut(s) 200, 317, 522
TseI GCWGC 1 cut(s) 529
TspDTI ATGAA 2 cut(s) 196, 578
TspRI CASTG 3 cut(s) 200, 317, 522
VneI GTGCAC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.