Rroxscaffold_2G00130920

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015316
Physical Location & Seq
Forward (+)
67427219 .. 67429604
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130920.1

Sequence Viewer

Length: 876 bp
ATGCCGATGTGTTTCAGCTTGATCGCCTCGCGCGACTCCTGCTTCCGCTACTCCTTCTCCTCCGCCGGCCTCAAGTCCACCACCACCGATCTCGGCGAAGGCACCATCATCCACAGCTGGTGGCACGAGTTCATCACCCCTCTCACTTCCCGCTTCAACCTCTACGTCCCCGACCTCATTTTCTTTGGGGACTCATACTCGTCCCGGAGCGAACGCTCCGAGACTTTCCAGGCCCAGTGCGTCATGGGCCTTATGGAGGCCCATGGCGTCAAGGGAAAAATGCACGTAGCCGGGATCAGCTACGGCGGGTTCGTGGCCTATAGCATGGTGGCTCAGTATGGGGAGAGGATTGAGAGGTTGGTGTTGTGTTGCGCTGGAGTTTGTGTCGAGGAGAAGGATATGGAGAAGGGGATGTTTCAGGTGAAGAGTGTGGAGGAGGCGGTGAGTATATTGCTGCCGCAGAGGGCGGCACAGGTGAGGCAGTTGATGAAGATCTCGTTTTACAAGCCGGCGACCAATGTGCCCTCTTGTTTTCTCAACGATTTTATTGAGGTAATGTGCACAGATCACGTTCAAGAGAGGAGGGAACTTATTCAAGCATTACATAAGGACAGAAAAATGTCTGATCTTCCCAAGATAACTCATCCTACACTAATAATCTGGGGGGAGCATGACCTGGTATTTCCAATGGAATTGGCACATCGATTAAAAAGGCAAATAGGTGAGAGTGCAGAACTAGTGACCATAAAGGATGCAGGACATGCCATAAATGTAGAGAAACCGAAAGAGATGCAGAAGTACATGAAGTCGTTCCTCCTTGATCCACTCCCTCAGCCTAAACAAGAAAAGCAAAGCAATGGCCACAAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

291

Amino Acids

33.08

Weight (kDa)

6.83

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 35 - 261 4.2e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 38 - 263 1.4e-12 Alpha/beta hydrolase family
Hydrolase_4 PF12146 87 - 260 7.8e-10 Serine aminopeptidase, S33
Abhydrolase_4 PF08386 212 - 275 6.6e-06 TAP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 868
AccII CGCG 2 cut(s) 31, 33
AciI CCGC 7 cut(s) 46, 63, 151, 306, 440, 458, 467
AclWI GGATC 2 cut(s) 302, 815
AcoI YGGCCR 1 cut(s) 859
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 868
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 2 cut(s) 256, 868
AgsI TTSAA 3 cut(s) 157, 575, 596
AhlI ACTAGT 1 cut(s) 736
AjnI CCWGG 2 cut(s) 228, 675
AluBI AGCT 3 cut(s) 18, 117, 300
AluI AGCT 3 cut(s) 18, 117, 300
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 1 cut(s) 215
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 2 cut(s) 302, 815
AoxI GGCC 6 cut(s) 67, 231, 247, 258, 315, 859
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 1 cut(s) 454
Asp700I GAANNNNTTC 2 cut(s) 591, 809
AspLEI GCGC 2 cut(s) 33, 374
AspS9I GGNCC 3 cut(s) 232, 247, 259
AsuC2I CCSGG 2 cut(s) 205, 292
AsuHPI GGTGA 5 cut(s) 127, 433, 454, 487, 734
BaeGI GKGCMC 2 cut(s) 525, 563
BalI TGGCCA 1 cut(s) 861
BanI GGYRCC 1 cut(s) 101
BauI CACGAG 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 563
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 1 cut(s) 441
BccI CCATC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 319
BcgI CGANNNNNNTGC 4 cut(s) 502, 536, 772, 806
BciT130I CCWGG 2 cut(s) 230, 677
BcnI CCSGG 2 cut(s) 205, 292
BcoDI GTCTC 1 cut(s) 215
BcuI ACTAGT 1 cut(s) 736
BfaI CTAG 1 cut(s) 737
BfmI CTRYAG 1 cut(s) 319
BglII AGATCT 1 cut(s) 492
BisI GCNGC 3 cut(s) 455, 458, 468
BlsI GCNGC 3 cut(s) 456, 459, 469
Bme1390I CCNGG 4 cut(s) 205, 230, 292, 677
BmgT120I GGNCC 3 cut(s) 232, 247, 259
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 4 cut(s) 205, 230, 292, 677
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 742, 780
BmuI ACTGGG 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 396
Bpu10I CCTNAGC 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 205, 292
Bsa29I ATCGAT 1 cut(s) 703
BsaAI YACGTR 1 cut(s) 286
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 262
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 369, 399
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 868
Bse118I RCCGGY 2 cut(s) 65, 508
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 862
Bse8I GATNNNNATC 1 cut(s) 491
BseBI CCWGG 2 cut(s) 230, 677
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 4 cut(s) 108, 417, 643, 757
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 2 cut(s) 256, 868
BseMI GCAATG 1 cut(s) 862
BseMII CTCAG 2 cut(s) 347, 845
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 4 cut(s) 49, 404, 449, 595
BseSI GKGCMC 2 cut(s) 525, 563
BseXI GCAGC 1 cut(s) 441
BsgI GTGCAG 1 cut(s) 750
Bsh1236I CGCG 2 cut(s) 31, 33
BshFI GGCC 6 cut(s) 69, 233, 249, 260, 317, 861
BshNI GGYRCC 1 cut(s) 101
BshVI ATCGAT 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 563
BsiSI CCGG 4 cut(s) 66, 205, 291, 509
BslFI GGGAC 3 cut(s) 152, 187, 203
BslI CCNNNNNNNGG 2 cut(s) 256, 868
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 3 cut(s) 152, 187, 203
BsnI GGCC 6 cut(s) 69, 233, 249, 260, 317, 861
Bsp1286I GDGCHC 2 cut(s) 525, 563
Bsp143I GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 7 cut(s) 46, 63, 151, 306, 440, 458, 467
BspANI GGCC 6 cut(s) 69, 233, 249, 260, 317, 861
BspCNI CTCAG 2 cut(s) 346, 844
BspDI ATCGAT 1 cut(s) 703
BspFNI CGCG 2 cut(s) 31, 33
BspLI GGNNCC 1 cut(s) 103
BspPI GGATC 2 cut(s) 302, 815
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 862
BsrFI RCCGGY 2 cut(s) 65, 508
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 2 cut(s) 65, 508
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 2 cut(s) 230, 677
Bst6I CTCTTC 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 2 cut(s) 67, 510
BstDEI CTNAG 2 cut(s) 333, 831
BstDSI CCRYGG 1 cut(s) 262
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 4 cut(s) 108, 417, 643, 757
BstFNI CGCG 2 cut(s) 31, 33
BstHHI GCGC 2 cut(s) 33, 374
BstKTI GATC 7 cut(s) 24, 91, 297, 495, 568, 628, 823
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
BstMWI GCNNNNNNNGC 3 cut(s) 39, 246, 761
BstNI CCWGG 2 cut(s) 230, 677
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 4 cut(s) 203, 228, 290, 675
BstSFI CTRYAG 1 cut(s) 319
BstSLI GKGCMC 2 cut(s) 525, 563
BstUI CGCG 2 cut(s) 31, 33
BstV1I GCAGC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 6 cut(s) 69, 233, 249, 260, 317, 861
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 4 cut(s) 108, 417, 643, 757
BtsIMutI CAGTG 1 cut(s) 242
Cac8I GCNNGC 2 cut(s) 67, 510
CfoI GCGC 2 cut(s) 33, 374
Cfr10I RCCGGY 2 cut(s) 65, 508
Cfr13I GGNCC 3 cut(s) 232, 247, 259
ClaI ATCGAT 1 cut(s) 703
CseI GACGC 2 cut(s) 229, 256
CsiI ACCWGGT 1 cut(s) 675
Csp6I GTAC 1 cut(s) 799
CviAII CATG 6 cut(s) 244, 263, 325, 671, 761, 802
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 2 cut(s) 333, 831
DpnI GATC 7 cut(s) 23, 90, 296, 494, 567, 627, 822
DpnII GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
DraIII CACNNNGTG 1 cut(s) 868
EaeI YGGCCR 1 cut(s) 859
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 262
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 228, 675
EcoT14I CCWWGG 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 6 cut(s) 247, 266, 328, 674, 764, 805
FaqI GGGAC 3 cut(s) 152, 187, 203
FatI CATG 6 cut(s) 243, 262, 324, 670, 760, 801
FauI CCCGC 2 cut(s) 158, 299
Fnu4HI GCNGC 3 cut(s) 455, 458, 468
FokI GGATG 4 cut(s) 95, 424, 630, 764
Fsp4HI GCNGC 3 cut(s) 455, 458, 468
FspBI CTAG 1 cut(s) 737
GlaI GCGC 2 cut(s) 32, 373
GluI GCNGC 3 cut(s) 455, 458, 468
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 6 cut(s) 69, 233, 249, 260, 317, 861
HapII CCGG 4 cut(s) 66, 205, 291, 509
HgaI GACGC 2 cut(s) 229, 256
HhaI GCGC 2 cut(s) 33, 374
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 247, 266, 328, 674, 764, 805
Hin6I GCGC 2 cut(s) 31, 372
HinP1I GCGC 2 cut(s) 31, 372
HinfI GANTC 2 cut(s) 35, 191
HpaII CCGG 4 cut(s) 66, 205, 291, 509
HphI GGTGA 5 cut(s) 127, 433, 454, 487, 734
Hpy166II GTNNAC 2 cut(s) 78, 561
Hpy188I TCNGA 2 cut(s) 220, 625
Hpy188III TCNNGA 1 cut(s) 575
Hpy8I GTNNAC 2 cut(s) 78, 561
HpyAV CCTTC 4 cut(s) 64, 92, 388, 400
HpyCH4IV ACGT 3 cut(s) 165, 285, 570
HpyCH4V TGCA 5 cut(s) 283, 561, 731, 755, 793
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 246, 761
HpyF3I CTNAG 2 cut(s) 333, 831
HpySE526I ACGT 3 cut(s) 165, 285, 570
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 247, 266, 328, 674, 764, 805
HspAI GCGC 2 cut(s) 31, 372
KroI GCCGGC 2 cut(s) 65, 508
KroNI GCCGGC 2 cut(s) 67, 510
Kzo9I GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
LmnI GCTCC 3 cut(s) 207, 221, 667
Lsp1109I GCAGC 1 cut(s) 441
LweI GCATC 2 cut(s) 742, 780
MabI ACCWGGT 1 cut(s) 675
MaeI CTAG 1 cut(s) 737
MaeII ACGT 3 cut(s) 165, 285, 570
MaeIII GTNAC 1 cut(s) 739
MalI GATC 7 cut(s) 23, 90, 296, 494, 567, 627, 822
MboI GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
MboII GAAGA 3 cut(s) 436, 502, 620
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 2 cut(s) 525, 563
MlsI TGGCCA 1 cut(s) 861
MluCI AATT 1 cut(s) 692
MluNI TGGCCA 1 cut(s) 861
MlyI GAGTC 2 cut(s) 29, 185
Mox20I TGGCCA 1 cut(s) 861
MroNI GCCGGC 2 cut(s) 65, 508
MroXI GAANNNNTTC 2 cut(s) 591, 809
MscI TGGCCA 1 cut(s) 861
MseI TTAA 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 861
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 4 cut(s) 66, 205, 291, 509
MspR9I CCNGG 4 cut(s) 205, 230, 292, 677
MvaI CCWGG 2 cut(s) 230, 677
MvnI CGCG 2 cut(s) 31, 33
MwoI GCNNNNNNNGC 3 cut(s) 39, 246, 761
NaeI GCCGGC 2 cut(s) 67, 510
NciI CCSGG 2 cut(s) 205, 292
NcoI CCATGG 1 cut(s) 262
NdeII GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
NgoMIV GCCGGC 2 cut(s) 65, 508
NlaIII CATG 6 cut(s) 247, 266, 328, 674, 764, 805
NlaIV GGNNCC 1 cut(s) 103
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 739
NspI RCATGY 1 cut(s) 764
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 309
PdiI GCCGGC 2 cut(s) 67, 510
PdmI GAANNNNTTC 2 cut(s) 591, 809
PflMI CCANNNNNTGG 1 cut(s) 868
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 3 cut(s) 456, 459, 469
PleI GAGTC 2 cut(s) 29, 185
PpsI GAGTC 2 cut(s) 29, 185
Ppu21I YACGTR 1 cut(s) 286
Psp6I CCWGG 2 cut(s) 228, 675
PspGI CCWGG 2 cut(s) 228, 675
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 3 cut(s) 232, 247, 259
PsuI RGATCY 1 cut(s) 492
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
SaqAI TTAA 1 cut(s) 707
SatI GCNGC 3 cut(s) 455, 458, 468
Sau3AI GATC 7 cut(s) 21, 88, 294, 492, 565, 625, 820
Sau96I GGNCC 3 cut(s) 232, 247, 259
SchI GAGTC 2 cut(s) 29, 185
ScrFI CCNGG 4 cut(s) 205, 230, 292, 677
SduI GDGCHC 2 cut(s) 525, 563
SexAI ACCWGGT 1 cut(s) 675
SfaNI GCATC 2 cut(s) 742, 780
SfcI CTRYAG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 736
Sse9I AATT 1 cut(s) 692
SsiI CCGC 7 cut(s) 46, 63, 151, 306, 440, 458, 467
SspMI CTAG 1 cut(s) 737
StyD4I CCNGG 4 cut(s) 203, 228, 290, 675
StyI CCWWGG 1 cut(s) 262
TaiI ACGT 3 cut(s) 168, 288, 573
TaqI TCGA 2 cut(s) 387, 703
TasI AATT 1 cut(s) 692
TatI WGTACW 1 cut(s) 798
TauI GCSGC 2 cut(s) 460, 470
Tru1I TTAA 1 cut(s) 707
Tru9I TTAA 1 cut(s) 707
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 3 cut(s) 121, 503, 818
TspRI CASTG 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 868
VneI GTGCAC 1 cut(s) 559
XagI CCTNNNNNAGG 1 cut(s) 254
XceI RCATGY 1 cut(s) 764
XmnI GAANNNNTTC 2 cut(s) 591, 809
XspI CTAG 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.