RLG00000018032

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015316
Physical Location & Seq
Reverse (-)
24631435 .. 24633676
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018032

Sequence Viewer

Length: 951 bp
ATGCCGATGTGCTTCAGCTTGATCGCCTCGCGCGACTCCTGCTTCCGCTACTCCTTCTCCTCCGCCGGCCTTAAGTCCACCACCACCGATCTCGGCGAAGGCACCATCATCCACAGCTGGGTCCCCAAACACCCCTCCCCATCCAAACCCACCCTCCTCCTCATCCATGGCATCGGAGCCAACGCAATGTGGCAGTGGCACGAGTTCATCACCCCTCTCACTTCCCGCTTCAACCTCTACGTCCCCGACCTCATTTTCTTCGGGGACTCCTATTCTTCCCGGAGTGAACGCTCCGAGACTTTTCAGGCCCAGTGCGTCATGGGCCTCATGGAGGCCCATGGCGTCAGGGGAAAAATGCACGTAGCCGGGATCAGCTACGGCGGGTTTGTGGCCTATAGCATGGCGGCGCAGTATGGGGAGAGGATTGAGAGGTTGGTGTTGTGTTGCGCTGGAGTTTGTGCCGAGGAGAAGGATATGGAGAAGGGGATGTTTCAGGTGAAGAGTGTGGAGGAGGCGGTGAGTATATTGCTGCCGCAGAGGGCGGCGCAGGTGAGGCAGTTGATGAAGATCTCGTTTTACAAGCCGGCGACCAATGTGCCCTCTTGTTTTCTCAACGATTTTATTGAGGTAATGTGCACAGATCACGTTCAAGAGAGGAGGGAACTTATTCAAGCATTACATAAGGACAGAAAAATGTCTGATCTTCCCAAGATAACTCATCCTACACTAATAATCTGGGGGGAGCATGACCTGGTATTTCCAATGGAATTGGCACATAGATTAAAAAGGCAAATAGGTGAGAGTGCAGAACTAGTGACCATAAAGGATGCAGGACATGCTATAAATGTAGAGAAACCGAAAGAGATGCAGAAGTACATGAAGTCGTTCCTCCTTGATCCACTCCCTCAGCCTAAAGAAGAAAAGCAAAGCAATGGCCACAAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

317

Amino Acids

35.72

Weight (kDa)

7.2

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 49 - 285 4.4e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 50 - 286 1.5e-20 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 288 2.1e-16 Alpha/beta hydrolase family
Abhydrolase_4 PF08386 237 - 300 7.6e-06 TAP-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 538
Acc36I ACCTGC 1 cut(s) 538
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 943
AccII CGCG 2 cut(s) 31, 33
AciI CCGC 8 cut(s) 46, 63, 226, 381, 404, 515, 533, 542
AclWI GGATC 2 cut(s) 377, 890
AcoI YGGCCR 1 cut(s) 934
AcyI GRCGYC 1 cut(s) 342
AdeI CACNNNGTG 1 cut(s) 943
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 3 cut(s) 118, 331, 943
AflII CTTAAG 1 cut(s) 71
AgsI TTSAA 3 cut(s) 232, 650, 671
AhlI ACTAGT 1 cut(s) 811
AjnI CCWGG 1 cut(s) 750
AluBI AGCT 3 cut(s) 18, 117, 375
AluI AGCT 3 cut(s) 18, 117, 375
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 1 cut(s) 290
Alw44I GTGCAC 1 cut(s) 634
AlwI GGATC 2 cut(s) 377, 890
AoxI GGCC 6 cut(s) 67, 306, 322, 333, 390, 934
ApaLI GTGCAC 1 cut(s) 634
ApeKI GCWGC 1 cut(s) 529
Asp700I GAANNNNTTC 2 cut(s) 666, 884
AspLEI GCGC 4 cut(s) 33, 409, 449, 547
AspS9I GGNCC 4 cut(s) 121, 307, 322, 334
AsuC2I CCSGG 2 cut(s) 280, 367
AsuHPI GGTGA 5 cut(s) 202, 508, 529, 562, 809
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 2 cut(s) 600, 638
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 1 cut(s) 101
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 638
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 1 cut(s) 516
BccI CCATC 2 cut(s) 113, 148
BceAI ACGGC 1 cut(s) 394
BcgI CGANNNNNNTGC 4 cut(s) 577, 611, 847, 881
BciT130I CCWGG 1 cut(s) 752
BcnI CCSGG 2 cut(s) 280, 367
BcoDI GTCTC 1 cut(s) 290
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 1 cut(s) 812
BfmI CTRYAG 1 cut(s) 394
BfrI CTTAAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 538
BglII AGATCT 1 cut(s) 567
BisI GCNGC 4 cut(s) 405, 530, 533, 543
BlsI GCNGC 4 cut(s) 406, 531, 534, 544
Bme1390I CCNGG 3 cut(s) 280, 367, 752
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 4 cut(s) 121, 307, 322, 334
BmiI GGNNCC 4 cut(s) 103, 122, 123, 178
BmrFI CCNGG 3 cut(s) 280, 367, 752
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 180, 817, 855
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 906
BpuMI CCSGG 2 cut(s) 280, 367
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 3 cut(s) 166, 337, 462
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 138, 168
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 331, 943
Bse118I RCCGGY 2 cut(s) 65, 583
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 2 cut(s) 192, 937
Bse8I GATNNNNATC 1 cut(s) 566
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 3 cut(s) 166, 337, 462
BseGI GGATG 6 cut(s) 108, 140, 162, 492, 718, 832
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 3 cut(s) 118, 331, 943
BseMI GCAATG 2 cut(s) 192, 937
BseMII CTCAG 1 cut(s) 920
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 6 cut(s) 49, 146, 149, 479, 524, 670
BseSI GKGCMC 2 cut(s) 600, 638
BseXI GCAGC 1 cut(s) 516
BseYI CCCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 2 cut(s) 31, 33
BshFI GGCC 6 cut(s) 69, 308, 324, 335, 392, 936
BshNI GGYRCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 638
BsiSI CCGG 4 cut(s) 66, 280, 366, 584
BslFI GGGAC 3 cut(s) 107, 227, 278
BslI CCNNNNNNNGG 3 cut(s) 118, 331, 943
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 3 cut(s) 107, 227, 278
BsnI GGCC 6 cut(s) 69, 308, 324, 335, 392, 936
Bsp1286I GDGCHC 2 cut(s) 600, 638
Bsp143I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
Bsp19I CCATGG 2 cut(s) 166, 337
BspACI CCGC 8 cut(s) 46, 63, 226, 381, 404, 515, 533, 542
BspANI GGCC 6 cut(s) 69, 308, 324, 335, 392, 936
BspCNI CTCAG 1 cut(s) 919
BspFNI CGCG 2 cut(s) 31, 33
BspLI GGNNCC 4 cut(s) 103, 122, 123, 178
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 2 cut(s) 377, 890
BspT107I GGYRCC 1 cut(s) 101
BspTI CTTAAG 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 192, 937
BsrFI RCCGGY 2 cut(s) 65, 583
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 65, 583
BssECI CCNNGG 3 cut(s) 166, 337, 462
BssMI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BssNI GRCGYC 1 cut(s) 342
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 2 cut(s) 166, 337
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 752
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 1 cut(s) 342
BstAFI CTTAAG 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 836
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 67, 585
BstDEI CTNAG 1 cut(s) 906
BstDSI CCRYGG 2 cut(s) 166, 337
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 6 cut(s) 108, 140, 162, 492, 718, 832
BstFNI CGCG 2 cut(s) 31, 33
BstHHI GCGC 4 cut(s) 33, 409, 449, 547
BstKTI GATC 7 cut(s) 24, 91, 372, 570, 643, 703, 898
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
BstMWI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 3 cut(s) 278, 365, 750
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 600, 638
BstUI CGCG 2 cut(s) 31, 33
BstV1I GCAGC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BsuRI GGCC 6 cut(s) 69, 308, 324, 335, 392, 936
BtgI CCRYGG 2 cut(s) 166, 337
BtsCI GGATG 6 cut(s) 108, 140, 162, 492, 718, 832
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 317
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 2 cut(s) 67, 585
CfoI GCGC 4 cut(s) 33, 409, 449, 547
Cfr10I RCCGGY 2 cut(s) 65, 583
Cfr13I GGNCC 4 cut(s) 121, 307, 322, 334
CseI GACGC 2 cut(s) 304, 331
CsiI ACCWGGT 1 cut(s) 750
Csp6I GTAC 1 cut(s) 874
CviAII CATG 8 cut(s) 167, 319, 328, 338, 400, 746, 836, 877
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 1 cut(s) 906
DpnI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
DpnII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
DraIII CACNNNGTG 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 166, 337
Eco47I GGWCC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 166, 337
ErhI CCWWGG 2 cut(s) 166, 337
FaeI CATG 8 cut(s) 170, 322, 331, 341, 403, 749, 839, 880
FaqI GGGAC 3 cut(s) 107, 227, 278
FatI CATG 8 cut(s) 166, 318, 327, 337, 399, 745, 835, 876
FauI CCCGC 2 cut(s) 233, 374
Fnu4HI GCNGC 4 cut(s) 405, 530, 533, 543
FokI GGATG 6 cut(s) 95, 127, 149, 499, 705, 839
Fsp4HI GCNGC 4 cut(s) 405, 530, 533, 543
FspBI CTAG 1 cut(s) 812
GlaI GCGC 4 cut(s) 32, 408, 448, 546
GluI GCNGC 4 cut(s) 405, 530, 533, 543
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 6 cut(s) 69, 308, 324, 335, 392, 936
HapII CCGG 4 cut(s) 66, 280, 366, 584
HgaI GACGC 2 cut(s) 304, 331
HhaI GCGC 4 cut(s) 33, 409, 449, 547
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 8 cut(s) 170, 322, 331, 341, 403, 749, 839, 880
Hin6I GCGC 4 cut(s) 31, 407, 447, 545
HinP1I GCGC 4 cut(s) 31, 407, 447, 545
HinfI GANTC 2 cut(s) 35, 266
HpaII CCGG 4 cut(s) 66, 280, 366, 584
HphI GGTGA 5 cut(s) 202, 508, 529, 562, 809
Hpy166II GTNNAC 3 cut(s) 78, 287, 636
Hpy188I TCNGA 3 cut(s) 176, 295, 700
Hpy188III TCNNGA 1 cut(s) 650
Hpy8I GTNNAC 3 cut(s) 78, 287, 636
HpyAV CCTTC 4 cut(s) 64, 92, 463, 475
HpyCH4IV ACGT 3 cut(s) 240, 360, 645
HpyCH4V TGCA 5 cut(s) 358, 636, 806, 830, 868
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
HpyF3I CTNAG 1 cut(s) 906
HpySE526I ACGT 3 cut(s) 240, 360, 645
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 8 cut(s) 170, 322, 331, 341, 403, 749, 839, 880
HspAI GCGC 4 cut(s) 31, 407, 447, 545
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 2 cut(s) 65, 583
KroNI GCCGGC 2 cut(s) 67, 585
Kzo9I GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
LmnI GCTCC 3 cut(s) 176, 296, 742
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 3 cut(s) 180, 817, 855
MabI ACCWGGT 1 cut(s) 750
MaeI CTAG 1 cut(s) 812
MaeII ACGT 3 cut(s) 240, 360, 645
MaeIII GTNAC 1 cut(s) 814
MalI GATC 7 cut(s) 23, 90, 371, 569, 642, 702, 897
MboI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
MboII GAAGA 6 cut(s) 250, 267, 511, 577, 695, 929
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 600, 638
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 2 cut(s) 29, 260
Mox20I TGGCCA 1 cut(s) 936
MroNI GCCGGC 2 cut(s) 65, 583
MroXI GAANNNNTTC 2 cut(s) 666, 884
MscI TGGCCA 1 cut(s) 936
MseI TTAA 2 cut(s) 72, 782
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 1 cut(s) 117
MspCI CTTAAG 1 cut(s) 71
MspI CCGG 4 cut(s) 66, 280, 366, 584
MspR9I CCNGG 3 cut(s) 280, 367, 752
MvaI CCWGG 1 cut(s) 752
MvnI CGCG 2 cut(s) 31, 33
MwoI GCNNNNNNNGC 4 cut(s) 39, 321, 553, 836
NaeI GCCGGC 2 cut(s) 67, 585
NciI CCSGG 2 cut(s) 280, 367
NcoI CCATGG 2 cut(s) 166, 337
NdeII GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
NgoMIV GCCGGC 2 cut(s) 65, 583
NlaIII CATG 8 cut(s) 170, 322, 331, 341, 403, 749, 839, 880
NlaIV GGNNCC 4 cut(s) 103, 122, 123, 178
NmeAIII GCCGAG 2 cut(s) 72, 487
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 1 cut(s) 839
PaqCI CACCTGC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PdiI GCCGGC 2 cut(s) 67, 585
PdmI GAANNNNTTC 2 cut(s) 666, 884
PflMI CCANNNNNTGG 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 4 cut(s) 406, 531, 534, 544
PleI GAGTC 2 cut(s) 29, 260
PpsI GAGTC 2 cut(s) 29, 260
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 4 cut(s) 103, 122, 123, 178
PspPI GGNCC 4 cut(s) 121, 307, 322, 334
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
SaqAI TTAA 2 cut(s) 72, 782
SatI GCNGC 4 cut(s) 405, 530, 533, 543
Sau3AI GATC 7 cut(s) 21, 88, 369, 567, 640, 700, 895
Sau96I GGNCC 4 cut(s) 121, 307, 322, 334
SchI GAGTC 2 cut(s) 29, 260
ScrFI CCNGG 3 cut(s) 280, 367, 752
SduI GDGCHC 2 cut(s) 600, 638
SexAI ACCWGGT 1 cut(s) 750
SfaNI GCATC 3 cut(s) 180, 817, 855
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 811
Sse9I AATT 1 cut(s) 767
SsiI CCGC 8 cut(s) 46, 63, 226, 381, 404, 515, 533, 542
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 3 cut(s) 278, 365, 750
StyI CCWWGG 2 cut(s) 166, 337
TaiI ACGT 3 cut(s) 243, 363, 648
TasI AATT 1 cut(s) 767
TatI WGTACW 1 cut(s) 873
TauI GCSGC 3 cut(s) 407, 535, 545
Tru1I TTAA 2 cut(s) 72, 782
Tru9I TTAA 2 cut(s) 72, 782
TscAI CASTG 2 cut(s) 200, 317
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 3 cut(s) 196, 578, 893
TspRI CASTG 2 cut(s) 200, 317
Van91I CCANNNNNTGG 1 cut(s) 943
Vha464I CTTAAG 1 cut(s) 71
VneI GTGCAC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 329
XceI RCATGY 1 cut(s) 839
XmnI GAANNNNTTC 2 cut(s) 666, 884
XspI CTAG 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.