pycom01g05960

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015316
Physical Location & Seq
Forward (+)
5969639 .. 5972117
2479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05960.1

Sequence Viewer

Length: 948 bp
ATGCCGGGGTGCTTCAGCATAGTAGCGTCACGCGACCGCTGCTTCCGCTACTCCTTCTCCTCCTCCGGCCTAAAGTCCACCACCACCGATTTGGGCGATGGCACCGTCATGCACTGCTGGGCTCCCAAAAACCACTCCCCATCCAAACCCACCCTCCTCCTCATCCACGGCATCGGCGCCAACGCAATGTGGCAGTGGCACGACTTCGTCTCCCCTCTCGTCTCTCGCTTCAACCTCTACATCCCCGACCTCGTCTTCTTCGGCGACTCCTACTCCTCCCGCCCTGAACGTTCTGAGATTTTCCAGGCACGCTGCGTCATGGCCCTCATGGACGCCCACGGAGTCAAGAAAATGATGGTCACCGGCATCAGCTACGGCGGCTTCGTGGCCTACCGCATGGCGGCGCAGTTTGGGGAAAGAATAGACAGGCTGGTGCTGTGCTGCGCCGGGGTGTGCACGGAGGAAAAGGATATGGAGAATGGGATGTTTCAGGTGAAGAGCGTGGAGGAGGCCATGAATGTTTTGTTGCCCCAGAAGCCTCAGCATTTTAGGGACTTGATGAAGATCTCTTTTTACAAGCCTGTTAAGAATGTCCCCTCTTGTTTTCTCAATGATTTCATTGAGGTGATGTGTTCAGAACATCTTCAAGAGAGGAAAGAATTGATTCAAGCACTACACAAGGACAGGAAGTTGTCTGATCTTCCGAAGATAAACCATCCTACACTAATCATCTGGGGAGAGCATGACCTGGTATTCCCACTGGAATTGGCGCACCGATTGAAAAGGCAAATAGGTGAGAGTGCAGACCTAGTGATCATAAAGAACGCAGGGCATGCTATAAATGCAGAGAAACCGAAAGAGATGTGCAAGCACATGAAGTCTTTCCTCATGGATCCTCTACCTACAGCTAAACATAAAAACCACAGCAACGGCCGCAAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

316

Amino Acids

35.78

Weight (kDa)

8.73

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 50 - 285 1.4e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 288 1.5e-14 Alpha/beta hydrolase family
Hydrolase_4 PF12146 106 - 284 2.1e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 101, 176
AccII CGCG 1 cut(s) 33
AciI CCGC 7 cut(s) 37, 46, 280, 378, 394, 401, 934
AclI AACGTT 1 cut(s) 289
AclWI GGATC 2 cut(s) 887, 900
AcoI YGGCCR 1 cut(s) 931
AcyI GRCGYC 2 cut(s) 177, 333
AfiI CCNNNNNNNGG 2 cut(s) 400, 940
AgsI TTSAA 4 cut(s) 232, 647, 668, 781
AjnI CCWGG 2 cut(s) 303, 747
AluBI AGCT 2 cut(s) 372, 908
AluI AGCT 2 cut(s) 372, 908
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 214, 226
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 2 cut(s) 887, 900
AoxI GGCC 5 cut(s) 67, 321, 387, 510, 931
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 3 cut(s) 39, 312, 441
Asp700I GAANNNNTTC 3 cut(s) 642, 663, 881
AspLEI GCGC 4 cut(s) 179, 406, 446, 772
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 2 cut(s) 6, 448
AsuHPI GGTGA 4 cut(s) 352, 505, 637, 806
BaeGI GKGCMC 1 cut(s) 458
BamHI GGATCC 1 cut(s) 892
BanI GGYRCC 2 cut(s) 101, 176
BanII GRGCYC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 458
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 3 cut(s) 26, 299, 428
BccI CCATC 4 cut(s) 92, 148, 349, 723
BceAI ACGGC 3 cut(s) 184, 391, 946
BciT130I CCWGG 2 cut(s) 305, 749
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 2 cut(s) 6, 448
BcoDI GTCTC 2 cut(s) 214, 226
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 1 cut(s) 903
BfoI RGCGCY 1 cut(s) 180
BglII AGATCT 1 cut(s) 564
BisI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
BlsI GCNGC 6 cut(s) 41, 314, 380, 403, 443, 935
Bme1390I CCNGG 4 cut(s) 6, 305, 448, 749
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 4 cut(s) 103, 123, 178, 894
BmrFI CCNGG 4 cut(s) 6, 305, 448, 749
BmsI GCATC 2 cut(s) 180, 375
BpiI GAAGAC 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 540
BpuMI CCSGG 2 cut(s) 6, 448
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 177, 333
BsaJI CCNNGG 4 cut(s) 5, 166, 337, 447
BsaXI ACNNNNNCTCC 8 cut(s) 41, 71, 138, 168, 194, 224, 257, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 940
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 765
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 2 cut(s) 305, 749
BseDI CCNNGG 4 cut(s) 5, 166, 337, 447
BseGI GGATG 5 cut(s) 140, 162, 240, 489, 715
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 2 cut(s) 400, 940
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 285, 554
BseNI ACTGG 1 cut(s) 765
BseRI GAGGAG 6 cut(s) 49, 52, 146, 149, 265, 521
BseSI GKGCMC 1 cut(s) 458
BseX3I CGGCCG 1 cut(s) 931
BseXI GCAGC 3 cut(s) 26, 299, 428
BseYI CCCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 2 cut(s) 37, 934
BshFI GGCC 5 cut(s) 69, 323, 389, 512, 933
BshNI GGYRCC 2 cut(s) 101, 176
BsiEI CGRYCG 2 cut(s) 37, 934
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 4 cut(s) 5, 66, 363, 447
BslFI GGGAC 2 cut(s) 566, 578
BslI CCNNNNNNNGG 2 cut(s) 400, 940
BsmAI GTCTC 2 cut(s) 214, 226
BsmBI CGTCTC 2 cut(s) 214, 226
BsmFI GGGAC 2 cut(s) 566, 578
BsnI GGCC 5 cut(s) 69, 323, 389, 512, 933
Bsp1286I GDGCHC 2 cut(s) 124, 458
Bsp143I GATC 4 cut(s) 564, 697, 813, 892
BspACI CCGC 7 cut(s) 37, 46, 280, 378, 394, 401, 934
BspANI GGCC 5 cut(s) 69, 323, 389, 512, 933
BspCNI CTCAG 2 cut(s) 286, 553
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 4 cut(s) 103, 123, 178, 894
BspPI GGATC 2 cut(s) 887, 900
BspQI GCTCTTC 1 cut(s) 491
BspT107I GGYRCC 2 cut(s) 101, 176
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 765
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 4 cut(s) 5, 166, 337, 447
BssMI GATC 4 cut(s) 564, 697, 813, 892
BssNI GRCGYC 2 cut(s) 177, 333
Bst2UI CCWGG 2 cut(s) 305, 749
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 2 cut(s) 177, 333
BstAPI GCANNNNNTGC 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 310, 834, 869
BstDEI CTNAG 2 cut(s) 294, 540
BstDSI CCRYGG 2 cut(s) 166, 337
BstEII GGTNACC 1 cut(s) 358
BstF5I GGATG 5 cut(s) 140, 162, 240, 489, 715
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 4 cut(s) 179, 406, 446, 772
BstKTI GATC 4 cut(s) 567, 700, 816, 895
BstMAI GTCTC 2 cut(s) 214, 226
BstMBI GATC 4 cut(s) 564, 697, 813, 892
BstMCI CGRYCG 2 cut(s) 37, 934
BstMWI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
BstNI CCWGG 2 cut(s) 305, 749
BstNSI RCATGY 1 cut(s) 836
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 4 cut(s) 4, 303, 446, 747
BstSFI CTRYAG 1 cut(s) 903
BstSLI GKGCMC 1 cut(s) 458
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 26, 299, 428
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 564, 892
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 2 cut(s) 564, 892
BstZI CGGCCG 1 cut(s) 931
BsuRI GGCC 5 cut(s) 69, 323, 389, 512, 933
BtgI CCRYGG 2 cut(s) 166, 337
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 5 cut(s) 140, 162, 240, 489, 715
BtsI GCAGTG 2 cut(s) 112, 200
BtsIMutI CAGTG 3 cut(s) 112, 200, 758
Cac8I GCNNGC 3 cut(s) 310, 834, 869
CfoI GCGC 4 cut(s) 179, 406, 446, 772
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 322
CseI GACGC 3 cut(s) 15, 304, 341
CsiI ACCWGGT 1 cut(s) 747
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 9 cut(s) 109, 319, 328, 397, 514, 743, 833, 874, 889
DdeI CTNAG 2 cut(s) 294, 540
DinI GGCGCC 1 cut(s) 178
DpnI GATC 4 cut(s) 566, 699, 815, 894
DpnII GATC 4 cut(s) 564, 697, 813, 892
EaeI YGGCCR 1 cut(s) 931
EagI CGGCCG 1 cut(s) 931
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
EclXI CGGCCG 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 124
Eco52I CGGCCG 1 cut(s) 931
Eco91I GGTNACC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 303, 747
EcoT38I GRGCYC 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
Esp3I CGTCTC 2 cut(s) 214, 226
FaeI CATG 9 cut(s) 112, 322, 331, 400, 517, 746, 836, 877, 892
FaqI GGGAC 2 cut(s) 566, 578
FatI CATG 9 cut(s) 108, 318, 327, 396, 513, 742, 832, 873, 888
FauI CCCGC 1 cut(s) 287
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
FokI GGATG 5 cut(s) 127, 149, 227, 496, 702
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
FspBI CTAG 1 cut(s) 809
GlaI GCGC 4 cut(s) 178, 405, 445, 771
GluI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 5 cut(s) 69, 323, 389, 512, 933
HapII CCGG 4 cut(s) 5, 66, 363, 447
HgaI GACGC 3 cut(s) 15, 304, 341
HhaI GCGC 4 cut(s) 179, 406, 446, 772
Hin1I GRCGYC 2 cut(s) 177, 333
Hin1II CATG 9 cut(s) 112, 322, 331, 400, 517, 746, 836, 877, 892
Hin6I GCGC 4 cut(s) 177, 404, 444, 770
HinP1I GCGC 4 cut(s) 177, 404, 444, 770
HinfI GANTC 3 cut(s) 266, 342, 664
HpaII CCGG 4 cut(s) 5, 66, 363, 447
HphI GGTGA 4 cut(s) 352, 505, 637, 806
Hpy166II GTNNAC 2 cut(s) 78, 456
Hpy188I TCNGA 4 cut(s) 295, 637, 697, 705
Hpy188III TCNNGA 2 cut(s) 346, 647
Hpy8I GTNNAC 2 cut(s) 78, 456
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 5 cut(s) 112, 456, 803, 845, 867
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
HpyF3I CTNAG 2 cut(s) 294, 540
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 2 cut(s) 177, 333
Hsp92II CATG 9 cut(s) 112, 322, 331, 400, 517, 746, 836, 877, 892
HspAI GCGC 4 cut(s) 177, 404, 444, 770
KasI GGCGCC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 4 cut(s) 564, 697, 813, 892
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 3 cut(s) 26, 299, 428
LweI GCATC 2 cut(s) 180, 375
MabI ACCWGGT 1 cut(s) 747
MaeI CTAG 1 cut(s) 809
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 2 cut(s) 27, 358
MalI GATC 4 cut(s) 566, 699, 815, 894
MboI GATC 4 cut(s) 564, 697, 813, 892
MboII GAAGA 7 cut(s) 247, 250, 508, 574, 635, 692, 718
MflI RGATCY 2 cut(s) 564, 892
MhlI GDGCHC 2 cut(s) 124, 458
MluCI AATT 2 cut(s) 659, 764
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 2 cut(s) 260, 351
MroXI GAANNNNTTC 3 cut(s) 642, 663, 881
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 2 cut(s) 107, 623
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 4 cut(s) 5, 66, 363, 447
MspR9I CCNGG 4 cut(s) 6, 305, 448, 749
MvaI CCWGG 2 cut(s) 305, 749
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 6, 448
NdeII GATC 4 cut(s) 564, 697, 813, 892
NlaIII CATG 9 cut(s) 112, 322, 331, 400, 517, 746, 836, 877, 892
NlaIV GGNNCC 4 cut(s) 103, 123, 178, 894
NmuCI GTSAC 2 cut(s) 27, 358
NspI RCATGY 1 cut(s) 836
PaeI GCATGC 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 3 cut(s) 642, 663, 881
PfeI GAWTC 1 cut(s) 664
PflFI GACNNNGTC 2 cut(s) 206, 251
PkrI GCNGC 6 cut(s) 41, 314, 380, 403, 443, 935
PleI GAGTC 2 cut(s) 260, 350
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 2 cut(s) 260, 350
Psp1406I AACGTT 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 303, 747
PspEI GGTNACC 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 303, 747
PspN4I GGNNCC 4 cut(s) 103, 123, 178, 894
PspPI GGNCC 1 cut(s) 322
PsuI RGATCY 2 cut(s) 564, 892
PsyI GACNNNGTC 2 cut(s) 206, 251
RseI CAYNNNNRTG 2 cut(s) 107, 623
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
Sau3AI GATC 4 cut(s) 564, 697, 813, 892
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 2 cut(s) 260, 351
ScrFI CCNGG 4 cut(s) 6, 305, 448, 749
SduI GDGCHC 2 cut(s) 124, 458
SexAI ACCWGGT 1 cut(s) 747
SfaNI GCATC 2 cut(s) 180, 375
SfcI CTRYAG 1 cut(s) 903
SfoI GGCGCC 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 107, 623
SphI GCATGC 1 cut(s) 836
Sse9I AATT 2 cut(s) 659, 764
SsiI CCGC 7 cut(s) 37, 46, 280, 378, 394, 401, 934
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 1 cut(s) 809
StyD4I CCNGG 4 cut(s) 4, 303, 446, 747
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 292
TasI AATT 2 cut(s) 659, 764
TauI GCSGC 3 cut(s) 381, 404, 936
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 3 cut(s) 119, 200, 765
TseFI GTSAC 2 cut(s) 27, 358
TseI GCWGC 3 cut(s) 39, 312, 441
Tsp45I GTSAC 2 cut(s) 27, 358
TspDTI ATGAA 4 cut(s) 530, 575, 607, 890
TspGWI ACGGA 2 cut(s) 354, 473
TspRI CASTG 3 cut(s) 119, 200, 765
Tth111I GACNNNGTC 2 cut(s) 206, 251
VneI GTGCAC 1 cut(s) 454
XceI RCATGY 1 cut(s) 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmnI GAANNNNTTC 3 cut(s) 642, 663, 881
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.