MD01G1032400.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015316
Physical Location & Seq
Forward (+)
11301276 .. 11304214
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1032400.v1.1.491

Sequence Viewer

Length: 948 bp
ATGCCGGGGTGCTTCAGCATAATAGCGTCACGCGACCGCTGCTTGCGCTACTCCTTCTCCTCCTCCGGCCTAAAGTCCACCACCACCGATTTGGGCGATGGCACCGTCATGCACTGCTGGGCTCCCAAAAACCACTCCCCATCCAAACCCACCCTCCTCCTCATCCACGGCATCGGCGCCAACGCAATGTGGCAGTGGCACGACTTCGTCTCCCCTCTCGCCTCCCGCTTCAACCTCTACATCCCCGACCTCGTCTTCTTCGGCGACTCCTACTCCTCCCGCCCTGAACGTTCTGAGATTTTCCAGGCACGCTGCGTCATGGCCCTCATGGACGCCCACGGAGTGAAGAAAATGATGGTCACCGGCATCAGCTACGGCGGCTTCGTGGCCTACCGCATGGCGGCGCAGTTTGGGGAGAGGATAGACAGGCTGGTGCTGTGCTGCGCCGGAGTGTGCACGGAGGAAAAGGATATGGAGAATGGGATGTTTCAGGTGAAGAGCGTGGAGGAGGCCATGAATGTTTTGTTGCCCCAGAAGCCTCAGCATGTTAGGGACTTGATGAAGATCTCTTTTTACAAGCCTGTTAAGAATGTCCCCTCTTGTTTTCTCAATGATTTTATTGAGGTGATGTGTTCAGAACATCTTCAAGAGAGGAAAGAATTGATTCAAGCACTGCACAAGGACAGGAAGTTGTCTGATCTTCCGAAGATAAACCATCCAACACTAATCATCTGGGGAGAGCATGACCTGGTATTCCCACTGGAATTGGCGCACCGATTAAAAAGGCAAATAGGTGAGAGTGCAAACCTAGTGATCATAAAGAACGCAGGGCATGCTATAAATGCAGAGAAACCGAAAGAGATGTGCAAGCACATGAAGTCTTTCCTCATCGATCCTCTCCCTACAGCTAAACATGAAAACCACAGCAACGGCCGCAAGGTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_4 Abhydrolase_6

Protein Analysis

316

Amino Acids

35.67

Weight (kDa)

8.61

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 49 - 284 3.8e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 50 - 285 3.3e-23 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 52 - 288 4.3e-15 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39955
fragaria_vesca FvH4_1g20610
malus_domestica MD01G1032400.v1.1
prunus_persica Prupe.6G189600_v2.0.a1
pyrus_communis pycom01g05960
rosa_chinensis RchiOBHm_Chr2g0113041
rosa_laevigata RLG00000018032
rosa_multiflora Rmu_co8372391.1_g000001 Rmu_sc0002351.1_g000106
rosa_roxburghii Rroxscaffold_2G00130920
rosa_rugosa Rorug02G0183400
rosa_samantha Rh2AG238600 Rh2BG252400 Rh2CG242600 Rh2DG246600
rosa_wichuraiana Rw2G018710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 101, 176
AccII CGCG 1 cut(s) 33
AciI CCGC 7 cut(s) 37, 226, 280, 378, 394, 401, 934
AclI AACGTT 1 cut(s) 289
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 931
AcyI GRCGYC 2 cut(s) 177, 333
AdeI CACNNNGTG 1 cut(s) 343
AfiI CCNNNNNNNGG 2 cut(s) 400, 940
AgsI TTSAA 3 cut(s) 232, 647, 668
AjnI CCWGG 2 cut(s) 303, 747
AluBI AGCT 2 cut(s) 372, 908
AluI AGCT 2 cut(s) 372, 908
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 214
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 1 cut(s) 887
AoxI GGCC 5 cut(s) 67, 321, 387, 510, 931
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 3 cut(s) 39, 312, 441
Asp700I GAANNNNTTC 3 cut(s) 642, 663, 881
AspLEI GCGC 5 cut(s) 48, 179, 406, 446, 772
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 4 cut(s) 352, 505, 637, 806
BaeGI GKGCMC 1 cut(s) 458
BanI GGYRCC 2 cut(s) 101, 176
BanII GRGCYC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 458
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 3 cut(s) 26, 299, 428
BccI CCATC 4 cut(s) 92, 148, 349, 723
BceAI ACGGC 3 cut(s) 184, 391, 946
BciT130I CCWGG 2 cut(s) 305, 749
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 1 cut(s) 809
BfmI CTRYAG 1 cut(s) 903
BfoI RGCGCY 1 cut(s) 180
BglII AGATCT 1 cut(s) 564
BisI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
BlsI GCNGC 6 cut(s) 41, 314, 380, 403, 443, 935
Bme1390I CCNGG 3 cut(s) 6, 305, 749
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 3 cut(s) 103, 123, 178
BmrFI CCNGG 3 cut(s) 6, 305, 749
BmsI GCATC 2 cut(s) 180, 375
BpiI GAAGAC 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 891
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 177, 333
BsaJI CCNNGG 3 cut(s) 5, 166, 337
BsaXI ACNNNNNCTCC 8 cut(s) 41, 71, 138, 168, 194, 224, 257, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 940
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 765
Bse3DI GCAATG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 2 cut(s) 305, 749
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 5, 166, 337
BseGI GGATG 5 cut(s) 140, 162, 240, 489, 715
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 2 cut(s) 400, 940
BseMI GCAATG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 285, 554
BseNI ACTGG 1 cut(s) 765
BseRI GAGGAG 6 cut(s) 49, 52, 146, 149, 265, 521
BseSI GKGCMC 1 cut(s) 458
BseX3I CGGCCG 1 cut(s) 931
BseXI GCAGC 3 cut(s) 26, 299, 428
BseYI CCCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 659
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 2 cut(s) 37, 934
BshFI GGCC 5 cut(s) 69, 323, 389, 512, 933
BshNI GGYRCC 2 cut(s) 101, 176
BshVI ATCGAT 1 cut(s) 891
BsiEI CGRYCG 2 cut(s) 37, 934
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 4 cut(s) 5, 66, 363, 447
BslFI GGGAC 2 cut(s) 566, 578
BslI CCNNNNNNNGG 2 cut(s) 400, 940
BsmAI GTCTC 1 cut(s) 214
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 2 cut(s) 566, 578
BsnI GGCC 5 cut(s) 69, 323, 389, 512, 933
Bsp1286I GDGCHC 2 cut(s) 124, 458
Bsp143I GATC 4 cut(s) 564, 697, 813, 892
BspACI CCGC 7 cut(s) 37, 226, 280, 378, 394, 401, 934
BspANI GGCC 5 cut(s) 69, 323, 389, 512, 933
BspCNI CTCAG 2 cut(s) 286, 553
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 103, 123, 178
BspPI GGATC 1 cut(s) 887
BspQI GCTCTTC 1 cut(s) 491
BspT107I GGYRCC 2 cut(s) 101, 176
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 765
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 5, 166, 337
BssMI GATC 4 cut(s) 564, 697, 813, 892
BssNI GRCGYC 2 cut(s) 177, 333
Bst2UI CCWGG 2 cut(s) 305, 749
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 2 cut(s) 177, 333
BstAPI GCANNNNNTGC 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 44, 310, 834, 869
BstDEI CTNAG 2 cut(s) 294, 540
BstDSI CCRYGG 2 cut(s) 166, 337
BstEII GGTNACC 1 cut(s) 358
BstF5I GGATG 5 cut(s) 140, 162, 240, 489, 715
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 5 cut(s) 48, 179, 406, 446, 772
BstKTI GATC 4 cut(s) 567, 700, 816, 895
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 4 cut(s) 564, 697, 813, 892
BstMCI CGRYCG 2 cut(s) 37, 934
BstMWI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
BstNI CCWGG 2 cut(s) 305, 749
BstNSI RCATGY 2 cut(s) 548, 836
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 3 cut(s) 4, 303, 747
BstSFI CTRYAG 1 cut(s) 903
BstSLI GKGCMC 1 cut(s) 458
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 26, 299, 428
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 564
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 564
BstZI CGGCCG 1 cut(s) 931
Bsu15I ATCGAT 1 cut(s) 891
BsuRI GGCC 5 cut(s) 69, 323, 389, 512, 933
BsuTUI ATCGAT 1 cut(s) 891
BtgI CCRYGG 2 cut(s) 166, 337
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 5 cut(s) 140, 162, 240, 489, 715
BtsI GCAGTG 3 cut(s) 112, 200, 671
BtsIMutI CAGTG 4 cut(s) 112, 200, 671, 758
Cac8I GCNNGC 4 cut(s) 44, 310, 834, 869
CfoI GCGC 5 cut(s) 48, 179, 406, 446, 772
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 891
CseI GACGC 3 cut(s) 15, 304, 341
CsiI ACCWGGT 1 cut(s) 747
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
DdeI CTNAG 2 cut(s) 294, 540
DinI GGCGCC 1 cut(s) 178
DpnI GATC 4 cut(s) 566, 699, 815, 894
DpnII GATC 4 cut(s) 564, 697, 813, 892
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 931
EagI CGGCCG 1 cut(s) 931
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
EclXI CGGCCG 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 124
Eco52I CGGCCG 1 cut(s) 931
Eco91I GGTNACC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 303, 747
EcoT38I GRGCYC 1 cut(s) 124
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 214
FaqI GGGAC 2 cut(s) 566, 578
FauI CCCGC 2 cut(s) 233, 287
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
FokI GGATG 5 cut(s) 127, 149, 227, 496, 702
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
FspBI CTAG 1 cut(s) 809
GlaI GCGC 5 cut(s) 47, 178, 405, 445, 771
GluI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 5 cut(s) 69, 323, 389, 512, 933
HapII CCGG 4 cut(s) 5, 66, 363, 447
HgaI GACGC 3 cut(s) 15, 304, 341
HhaI GCGC 5 cut(s) 48, 179, 406, 446, 772
Hin1I GRCGYC 2 cut(s) 177, 333
Hin6I GCGC 5 cut(s) 46, 177, 404, 444, 770
HinP1I GCGC 5 cut(s) 46, 177, 404, 444, 770
HinfI GANTC 2 cut(s) 266, 664
HpaII CCGG 4 cut(s) 5, 66, 363, 447
HphI GGTGA 4 cut(s) 352, 505, 637, 806
Hpy166II GTNNAC 2 cut(s) 78, 456
Hpy188I TCNGA 4 cut(s) 295, 637, 697, 705
Hpy188III TCNNGA 1 cut(s) 647
Hpy8I GTNNAC 2 cut(s) 78, 456
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 6 cut(s) 112, 456, 676, 803, 845, 867
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
HpyF3I CTNAG 2 cut(s) 294, 540
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 2 cut(s) 177, 333
HspAI GCGC 5 cut(s) 46, 177, 404, 444, 770
KasI GGCGCC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 4 cut(s) 564, 697, 813, 892
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 3 cut(s) 26, 299, 428
LweI GCATC 2 cut(s) 180, 375
MabI ACCWGGT 1 cut(s) 747
MaeI CTAG 1 cut(s) 809
MaeII ACGT 1 cut(s) 289
MaeIII GTNAC 2 cut(s) 27, 358
MalI GATC 4 cut(s) 566, 699, 815, 894
MboI GATC 4 cut(s) 564, 697, 813, 892
MboII GAAGA 8 cut(s) 247, 250, 358, 508, 574, 635, 692, 718
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 2 cut(s) 124, 458
MluCI AATT 2 cut(s) 659, 764
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 743
MroXI GAANNNNTTC 3 cut(s) 642, 663, 881
MseI TTAA 2 cut(s) 585, 779
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 4 cut(s) 5, 66, 363, 447
MspR9I CCNGG 3 cut(s) 6, 305, 749
MvaI CCWGG 2 cut(s) 305, 749
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 7 cut(s) 39, 45, 378, 535, 833, 842, 933
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 1 cut(s) 6
NdeII GATC 4 cut(s) 564, 697, 813, 892
NlaIV GGNNCC 3 cut(s) 103, 123, 178
NmuCI GTSAC 2 cut(s) 27, 358
NspI RCATGY 2 cut(s) 548, 836
PaeI GCATGC 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 3 cut(s) 642, 663, 881
PfeI GAWTC 1 cut(s) 664
PflFI GACNNNGTC 2 cut(s) 206, 251
PkrI GCNGC 6 cut(s) 41, 314, 380, 403, 443, 935
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 260
Psp1406I AACGTT 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 303, 747
PspEI GGTNACC 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 303, 747
PspN4I GGNNCC 3 cut(s) 103, 123, 178
PspPI GGNCC 1 cut(s) 322
PsuI RGATCY 1 cut(s) 564
PsyI GACNNNGTC 2 cut(s) 206, 251
RseI CAYNNNNRTG 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 2 cut(s) 585, 779
SatI GCNGC 6 cut(s) 40, 313, 379, 402, 442, 934
Sau3AI GATC 4 cut(s) 564, 697, 813, 892
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 6, 305, 749
SduI GDGCHC 2 cut(s) 124, 458
SexAI ACCWGGT 1 cut(s) 747
SfaNI GCATC 2 cut(s) 180, 375
SfcI CTRYAG 1 cut(s) 903
SfoI GGCGCC 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 107
SphI GCATGC 1 cut(s) 836
Sse9I AATT 2 cut(s) 659, 764
SsiI CCGC 7 cut(s) 37, 226, 280, 378, 394, 401, 934
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 1 cut(s) 809
StyD4I CCNGG 3 cut(s) 4, 303, 747
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 292
TaqI TCGA 1 cut(s) 891
TasI AATT 2 cut(s) 659, 764
TauI GCSGC 3 cut(s) 381, 404, 936
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 2 cut(s) 585, 779
Tru9I TTAA 2 cut(s) 585, 779
TscAI CASTG 4 cut(s) 119, 200, 678, 765
TseFI GTSAC 2 cut(s) 27, 358
TseI GCWGC 3 cut(s) 39, 312, 441
Tsp45I GTSAC 2 cut(s) 27, 358
TspDTI ATGAA 4 cut(s) 530, 575, 890, 930
TspGWI ACGGA 2 cut(s) 354, 473
TspRI CASTG 4 cut(s) 119, 200, 678, 765
Tth111I GACNNNGTC 2 cut(s) 206, 251
VneI GTGCAC 1 cut(s) 454
XceI RCATGY 2 cut(s) 548, 836
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XmnI GAANNNNTTC 3 cut(s) 642, 663, 881
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.