FvH4_1g23130
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012455
Physical Location & Seq
Reverse (-)
14981276 .. 14984830
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23130.t1

Sequence Viewer

Length: 3555 bp
ATGAAACCCCACAGACCCGTTTCACCACCCTTCTTTCTTCTTCTGCTTCTTCTTCTCTTCTTCCTCTCTGCAACTCCTTCTTCTTCTGCAGCTTACAAAGACTCCCAGAACCTCTTGAGCTTCAAATACTCTCTTCCCAAACCTACCCTTCTCTCCAATTGGCTCCCTGACCAAAACCCATGTCTGTTCTCCGGCGTCTTCTGCAAACAAACCCGGGTCTCCTCCATCGACCTCAGCTTAATCCCCTTGTCCACCAACCTCACTGTCGTCTCCACTTTCTTAATGACCATTGATTCTCTTCAATCCCTCACTCTCAAAACCACCGCCCTCTCCGGCCCGGTTTCTTTTCCGGCGAAATCCAAGTGCAGCCCTCTCCTCACCTCCATAGATCTTGCTCAGAACACCTTGTCCGGCCCAATCTCTACTCTCTCCAACTTGGGCTCCTGCTCCGGTTTAAAGTCTCTCAACCTCTCCTCTAATCTACTCGATTTCAATGTCAAAGACTCCACCCCGTTCGGCCTCTCGCTCCATGTCCTCGACCTCTCCTTCAACAAGATTTCCGGCCCCGCCGTGCCGTGGATCTTATCCAACGGCTGTGCTGAGCTCGTGCAACTAGTCCTCAAGGGGAACAAGATCACCGGGGACATGAGTGTCTCCGGGTGCAAGAAGCTCGAGATTCTTGATTTCTCCTCGAACAATTTCACCCTCGAGATTCCATCGTTCGGCGACTGCTTGGTTCTCGACCGCCTCGACATCTCCGGGAACAAATTATCCGGCGACGTCGCAAATGCTCTCTCTTCCTGCAGCCACCTCACGTTTCTCAACCTCTCCATCAACCATTTCTCAGGCCAGATTCCGGCCGTTCCCGCCGAGAAGCTTAAGTTCCTCTCCCTCTCCGGGAACGAGTTCCAAGGTACAATTCCACCCAGCTTGCTGGGTTCATGCGAGTCGCTACTGGAGCTTGATCTTTCGATGAACAATCTCTCCGGTACTGTTCCCGACGCATTAAGCTCGTGTGCTTCGCTGGAGACTCTCGACATTTCCGGCAACTTCTTCACCGGCGAGCTGCCCGTCGAGACTTTATTGAAGCTCAGCAAGTTGAAGTCTGTGTCTCTTTCTCTCAACGATTTCGTGGGCACTCTGCCGCGTTCTCTCTCCAAGCTTGCGCATTTGGAGAGCTTGGATCTCAGCTCCAACAATTTCACCGGTTCTGTTCCGAGCTGGCTCTGTGAAGGTCCCGGGAACAGCTGGAAGGAGCTGTATCTTCAGAACAATAAGTTTGGCGGGACTATTCCTCCATCTATTAGCAACTGTACTCAGCTTGTGGCGCTAGATTTGAGCTTCAATTATCTCACTGGCACCATCCCTTCGAGCTTGGGGTCATTGTCGAAGCTGAGAGACTTGATCCTTTGGTTGAATCAGCTCAGCGGGGAGATTCCGCAGGAGCTGATGTATCTCGGGTCACTGGAGAATCTTATTCTCGACTTCAATGAGCTGACCGGGACGATTCCTGTTGGGCTGAGCAACTGCACCAACTTGAGCTGGATTTCGCTGGCCAACAACAAGTTGAGTGGCGAGATTCCTGCCTGGATTGGGAAGCTTCCGAAGCTGGCGATACTTAAGCTCAGTAACAACTCGTTCTATGGGAACATCCCACCGGAGCTGGGTGACTGTAAGAGCTTGATATGGTTGGATCTCAATACAAACTTGTTGAATGGTTCAATCCCTCCTGGGCTTTTCAAACAATCTGGCAACATTGCGGTGAATTTTGTTGCTTCCAAGACGTATGTGTATATCAAGAATGATGGAAGTAAGGAGTGCCATGGAGCAGGGAACTTGCTCGAGTTTGCTGGGATAAGGCAAGAGCAATTGACCAGGCTATCGACCAGAAACCCCTGCAACTTCACTAGAGTTTATAGAGGTATTCTCCAACCAACATTTAATCACAATGGGACGATGATTTTTCTTGATATTTCACATAATAGGTTATCCGGTAGCATTCCAAAAGAGATTGGGAGTATGTACTATCTCTATATTCTGAATTTGGGCCATAATAATATATCTGGAGCGATTCCAGAAGAGCTTGGGAAATTGAAGGACCTGAACATTCTTGATCTCTCGAGCAACAGCCTTGATGGAAGCATTCCTCAGACTTTGGTTGGCCTTTCCATGCTGATGGAGATTGATTTGTCAAACAATCATCTGTCTGGAATGATTCCAGATTCAGGGCAGTTTGAGACATTCCCTGCTTACAGATTCATGAACAATTCAGACCTTTGTGGCTACCCATTAAATCCCTGTGGAGCTGCTTCTGGGGCAAATGGAAATGGGCACCAGAAGTCTCATCGACAAGCGTCCTTGGCAGGCAGTGTGGCAATGGGGCTACTGTTCTCCCTTTTCTGCATCTTTGGTTTGCTTATTGTTTTAATCGAAACCAGGAAAAGGCGGAAAAAGAAGGATTCAAGTCTTGATGTTTATGTGGACAGTCGTTCCCACTCGGGTACTGCCTGGAAGCTAACAGGTGCCCGAGAAGCATTGAGCATTAACCTTTCCACATTTGAGAAGCCACTTCAAAAGCTCACATTTGCAGATCTTCTTGAGGCTACCAATGGTTTCCACAATGACAGTCTTATTGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAGGATGGCAGCATTGTAGCCATCAAGAAATTGATCCATATCAGTGGACAAGGTGATCGTGAATTCACTGCTGAAATGGAAACTATCGGGAAGATCAAGCACCGAAACCTTGTTCCGCTCCTTGGATACTGTAAAGTAGGAGAAGAACGGCTTTTAGTTTACGAGTACATGAAGTATGGGAGTTTAGATGATGTTTTACATGACCAGAAGAAAGGTATCAAGCTGAGTTGGTCTGCAAGGAGGAAGATTGCCATTGGGTCTGCAAGGGGACTGGCCTTTCTTCACCACAATTGCATTCCTCACATCATTCATAGGGACATGAAATCGAGCAACGTCCTGGTCGATGAGAACTTGGAAGCCAGGGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACTCATTTGAGTGTGAGCACTCTGGCAGGCACACCTGGTTATGTCCCTCCAGAATACTACCAGAGCTTCAGATGTTCTACAAAAGGCGATGTTTATAGTTATGGTGTAGTATTGCTCGAGCTGCTAACAGGAAGACGTCCTACAGATTCAGCTGATTTTGGTGACAATAATCTTGTTGGTTGGGTGAAACAGCATGCTAAATTAAAAATAAGTGATGTTTTTGATCCAGAGCTCATGAAAGAGGACCCGACCCTAGAGATTGAGCTGTTGCAGCACTTAAAGGTAGCTTGCGCCTGTTTGGATGATAGGCCGTGGCGCCGCCCTACGATGATTCAAGTCATGGCAATGTTCAAGGAGATCCAAGCAGGATCCGGAATGGATTCCCAGTCAACCATAGGCACTGACGATGGAGGTTTTGGTGCAGTTGAGATGGTTGAGATGAGCATAAAAGAAGACCCTGAACCGGGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1185

Amino Acids

128.87

Weight (kDa)

6.21

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 68 2.3e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 238 - 475 2.2e-10 Leucine-rich repeat region
LRR_8 PF13855 292 - 350 4.7e-08 Leucine rich repeat
LRR_14 PF23598 433 - 569 5.4e-06 Leucine-rich repeat region
LRR_8 PF13855 488 - 546 1.7e-07 Leucine rich repeat
Island PF20141 584 - 648 1.7e-31 Brassinosteroid receptor island domain
LRR_14 PF23598 662 - 732 2.3e-06 Leucine-rich repeat region
LRR_8 PF13855 677 - 735 7.1e-09 Leucine rich repeat
Pkinase PF00069 872 - 1141 1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 875 - 1140 7.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AatII GACGTC 2 cut(s) 783, 3217
Acc16I TGCGCA 1 cut(s) 1167
AccB1I GGYRCC 4 cut(s) 1358, 2331, 2522, 3393
AccBSI CCGCTC 1 cut(s) 2791
AccI GTMKAC 1 cut(s) 2658
AccII CGCG 1 cut(s) 1147
AccIII TCCGGA 1 cut(s) 3449
AclWI GGATC 9 cut(s) 587, 1191, 1399, 1701, 2701, 3296, 3430, 3441, 3454
AcoI YGGCCR 2 cut(s) 858, 1554
AcsI RAATTY 3 cut(s) 1765, 2041, 2735
AcuI CTGAAG 2 cut(s) 1250, 3130
AcyI GRCGYC 4 cut(s) 195, 780, 3214, 3394
AfaI GTAC 6 cut(s) 916, 991, 1315, 2024, 2503, 2840
AflII CTTAAG 2 cut(s) 878, 1619
AgeI ACCGGT 1 cut(s) 1205
AhlI ACTAGT 1 cut(s) 613
AjnI CCWGG 8 cut(s) 1586, 1729, 1874, 2435, 2507, 3009, 3032, 3112
AjuI GAANNNNNNNTTGG 2 cut(s) 903, 935
AloI GAACNNNNNNTCC 6 cut(s) 392, 424, 755, 787, 968, 1000
Alw21I GWGCWC 3 cut(s) 606, 3098, 3312
AlwI GGATC 9 cut(s) 587, 1191, 1399, 1701, 2701, 3296, 3430, 3441, 3454
AlwNI CAGNNNCTG 1 cut(s) 1447
Aor13HI TCCGGA 1 cut(s) 3449
ApeKI GCWGC 8 cut(s) 89, 366, 804, 1066, 2306, 2682, 3199, 3349
ApoI RAATTY 3 cut(s) 1765, 2041, 2735
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AsiGI ACCGGT 1 cut(s) 1205
Asp700I GAANNNNTTC 3 cut(s) 698, 905, 3457
AspLEI GCGC 4 cut(s) 1168, 1330, 3371, 3396
AspS9I GGNCC 8 cut(s) 335, 413, 563, 1235, 2047, 2098, 2665, 3322
AvaII GGWCC 3 cut(s) 1235, 2098, 3322
BaeGI GKGCMC 3 cut(s) 1139, 2334, 2527
BalI TGGCCA 1 cut(s) 1556
BamHI GGATCC 1 cut(s) 3446
BanI GGYRCC 4 cut(s) 1358, 2331, 2522, 3393
BanII GRGCYC 3 cut(s) 443, 606, 3312
BarI GAAGNNNNNNTAC 2 cut(s) 3202, 3234
BauI CACGAG 2 cut(s) 605, 1012
BbsI GAAGAC 3 cut(s) 190, 3217, 3537
Bbv12I GWGCWC 3 cut(s) 606, 3098, 3312
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 8 cut(s) 101, 378, 816, 1053, 2293, 2694, 3186, 3361
BceAI ACGGC 6 cut(s) 554, 559, 607, 845, 2837, 3373
BcgI CGANNNNNNTGC 6 cut(s) 652, 686, 993, 1027, 1565, 1599
BciT130I CCWGG 8 cut(s) 1588, 1731, 1876, 2437, 2509, 3011, 3034, 3114
BciVI GTATCC 1 cut(s) 2792
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 4 cut(s) 614, 1331, 1908, 3332
BfmI CTRYAG 3 cut(s) 87, 802, 3219
BfoI RGCGCY 2 cut(s) 1331, 3397
BfrI CTTAAG 2 cut(s) 878, 1619
BfuI GTATCC 1 cut(s) 2792
BglII AGATCT 2 cut(s) 388, 2590
BlpI GCTNAGC 4 cut(s) 600, 1091, 1424, 1520
Bme18I GGWCC 3 cut(s) 1235, 2098, 3322
BmgT120I GGNCC 8 cut(s) 335, 413, 563, 1235, 2047, 2098, 2665, 3322
BmrI ACTGGG 1 cut(s) 3457
BmsI GCATC 1 cut(s) 2412
BmuI ACTGGG 1 cut(s) 3457
BoxI GACNNNNGTC 1 cut(s) 2353
BpiI GAAGAC 3 cut(s) 190, 3217, 3537
BplI GAGNNNNNCTC 2 cut(s) 1911, 1943
BpmI CTGGAG 5 cut(s) 977, 1046, 1487, 2085, 3111
Bpu10I CCTNAGC 1 cut(s) 233
Bpu1102I GCTNAGC 4 cut(s) 600, 1091, 1424, 1520
BpuEI CTTGAG 4 cut(s) 136, 605, 1558, 2618
BsaBI GATNNNNATC 1 cut(s) 2711
BsaHI GRCGYC 4 cut(s) 195, 780, 3214, 3394
BsaI GGTCTC 2 cut(s) 223, 3043
BsaWI WCCGGW 6 cut(s) 449, 986, 1205, 1657, 1991, 3449
Bse118I RCCGGY 2 cut(s) 1058, 1205
Bse1I ACTGG 5 cut(s) 960, 1360, 1470, 2949, 3463
Bse3DI GCAATG 4 cut(s) 1755, 2382, 3078, 3429
Bse8I GATNNNNATC 1 cut(s) 2711
BseAI TCCGGA 1 cut(s) 3449
BseBI CCWGG 8 cut(s) 1588, 1731, 1876, 2437, 2509, 3011, 3034, 3114
BseGI GGATG 4 cut(s) 1362, 1650, 2683, 3385
BseJI GATNNNNATC 1 cut(s) 2711
BseMI GCAATG 4 cut(s) 1755, 2382, 3078, 3429
BseNI ACTGG 5 cut(s) 960, 1360, 1470, 2949, 3463
BseRI GAGGAG 4 cut(s) 211, 365, 463, 679
BseSI GKGCMC 3 cut(s) 1139, 2334, 2527
BseX3I CGGCCG 1 cut(s) 858
BseXI GCAGC 8 cut(s) 101, 378, 816, 1053, 2293, 2694, 3186, 3361
BseYI CCCAGC 4 cut(s) 926, 934, 1663, 1850
BsgI GTGCAG 3 cut(s) 385, 1513, 3519
Bsh1236I CGCG 1 cut(s) 1147
Bsh1285I CGRYCG 2 cut(s) 745, 861
BshNI GGYRCC 4 cut(s) 1358, 2331, 2522, 3393
BshTI ACCGGT 1 cut(s) 1205
BsiEI CGRYCG 2 cut(s) 745, 861
BsiHKAI GWGCWC 3 cut(s) 606, 3098, 3312
BslFI GGGAC 8 cut(s) 656, 1221, 1300, 1516, 1966, 2955, 3002, 3107
BsmBI CGTCTC 1 cut(s) 274
BsmFI GGGAC 8 cut(s) 656, 1221, 1300, 1516, 1966, 2955, 3002, 3107
BsmI GAATGC 3 cut(s) 1998, 2142, 2967
Bso31I GGTCTC 2 cut(s) 223, 3043
Bsp1286I GDGCHC 7 cut(s) 443, 606, 1139, 2334, 2527, 3098, 3312
Bsp13I TCCGGA 1 cut(s) 3449
Bsp1720I GCTNAGC 4 cut(s) 600, 1091, 1424, 1520
Bsp19I CCATGG 1 cut(s) 1822
BspEI TCCGGA 1 cut(s) 3449
BspFNI CGCG 1 cut(s) 1147
BspHI TCATGA 2 cut(s) 2259, 3312
BspMAI CTGCAG 2 cut(s) 91, 806
BspPI GGATC 9 cut(s) 587, 1191, 1399, 1701, 2701, 3296, 3430, 3441, 3454
BspQI GCTCTTC 1 cut(s) 2073
BspT107I GGYRCC 4 cut(s) 1358, 2331, 2522, 3393
BspTI CTTAAG 2 cut(s) 878, 1619
BspTNI GGTCTC 2 cut(s) 223, 3043
BsrBI CCGCTC 1 cut(s) 2791
BsrDI GCAATG 4 cut(s) 1755, 2382, 3078, 3429
BsrFI RCCGGY 2 cut(s) 1058, 1205
BsrI ACTGG 5 cut(s) 960, 1360, 1470, 2949, 3463
BssAI RCCGGY 2 cut(s) 1058, 1205
BssNAI GTATAC 1 cut(s) 2659
BssNI GRCGYC 4 cut(s) 195, 780, 3214, 3394
BssSI CACGAG 2 cut(s) 605, 1012
BssT1I CCWWGG 4 cut(s) 910, 1822, 2358, 2794
Bst1107I GTATAC 1 cut(s) 2659
Bst2BI CACGAG 2 cut(s) 605, 1012
Bst2UI CCWGG 8 cut(s) 1588, 1731, 1876, 2437, 2509, 3011, 3034, 3114
Bst4CI ACNGT 8 cut(s) 265, 994, 1313, 1673, 2388, 2486, 2627, 2804
Bst6I CTCTTC 5 cut(s) 62, 138, 303, 802, 2073
BstACI GRCGYC 4 cut(s) 195, 780, 3214, 3394
BstAFI CTTAAG 2 cut(s) 878, 1619
BstDSI CCRYGG 3 cut(s) 575, 1822, 3389
BstF5I GGATG 4 cut(s) 1362, 1650, 2683, 3385
BstFNI CGCG 1 cut(s) 1147
BstH2I RGCGCY 2 cut(s) 1331, 3397
BstHHI GCGC 4 cut(s) 1168, 1330, 3371, 3396
BstMCI CGRYCG 2 cut(s) 745, 861
BstNI CCWGG 8 cut(s) 1588, 1731, 1876, 2437, 2509, 3011, 3034, 3114
BstNSI RCATGY 1 cut(s) 3275
BstPAI GACNNNNGTC 1 cut(s) 2353
BstSFI CTRYAG 3 cut(s) 87, 802, 3219
BstSLI GKGCMC 3 cut(s) 1139, 2334, 2527
BstUI CGCG 1 cut(s) 1147
BstV1I GCAGC 8 cut(s) 101, 378, 816, 1053, 2293, 2694, 3186, 3361
BstV2I GAAGAC 3 cut(s) 190, 3217, 3537
BstX2I RGATCY 7 cut(s) 388, 579, 1183, 1693, 2590, 3435, 3446
BstXI CCANNNNNNTGG 3 cut(s) 934, 2176, 2717
BstYI RGATCY 7 cut(s) 388, 579, 1183, 1693, 2590, 3435, 3446
BstZ17I GTATAC 1 cut(s) 2659
BstZI CGGCCG 1 cut(s) 858
BsuI GTATCC 1 cut(s) 2792
BtgI CCRYGG 3 cut(s) 575, 1822, 3389
BtgZI GCGATG 1 cut(s) 3180
BtsCI GGATG 4 cut(s) 1362, 1650, 2683, 3385
BtsI GCAGTG 2 cut(s) 2374, 2739
BtsIMutI CAGTG 7 cut(s) 261, 1353, 1463, 2374, 2722, 2739, 3477
CaiI CAGNNNCTG 1 cut(s) 1447
CciI TCATGA 2 cut(s) 2259, 3312
CfoI GCGC 4 cut(s) 1168, 1330, 3371, 3396
Cfr10I RCCGGY 2 cut(s) 1058, 1205
Cfr13I GGNCC 8 cut(s) 335, 413, 563, 1235, 2047, 2098, 2665, 3322
Cfr9I CCCGGG 2 cut(s) 213, 1238
CseI GACGC 3 cut(s) 184, 1010, 2343
CsiI ACCWGGT 1 cut(s) 3112
Csp6I GTAC 6 cut(s) 915, 990, 1314, 2023, 2502, 2839
CspAI ACCGGT 1 cut(s) 1205
CspCI CAANNNNNGTGG 4 cut(s) 912, 947, 1552, 1587
CviQI GTAC 6 cut(s) 915, 990, 1314, 2023, 2502, 2839
DinI GGCGCC 1 cut(s) 3395
DraI TTTAAA 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 2 cut(s) 858, 1554
EagI CGGCCG 1 cut(s) 858
Eam1104I CTCTTC 5 cut(s) 62, 138, 303, 802, 2073
EarI CTCTTC 5 cut(s) 62, 138, 303, 802, 2073
EciI GGCGGA 1 cut(s) 2461
Ecl136II GAGCTC 2 cut(s) 604, 3310
EclXI CGGCCG 1 cut(s) 858
Eco130I CCWWGG 4 cut(s) 910, 1822, 2358, 2794
Eco24I GRGCYC 3 cut(s) 443, 606, 3312
Eco31I GGTCTC 2 cut(s) 223, 3043
Eco47I GGWCC 3 cut(s) 1235, 2098, 3322
Eco52I CGGCCG 1 cut(s) 858
Eco53kI GAGCTC 2 cut(s) 604, 3310
Eco57I CTGAAG 2 cut(s) 1250, 3130
EcoICRI GAGCTC 2 cut(s) 604, 3310
EcoO109I RGGNCCY 3 cut(s) 1235, 2098, 3322
EcoRI GAATTC 1 cut(s) 2735
EcoRII CCWGG 8 cut(s) 1586, 1729, 1874, 2435, 2507, 3009, 3032, 3112
EcoT14I CCWWGG 4 cut(s) 910, 1822, 2358, 2794
EcoT38I GRGCYC 3 cut(s) 443, 606, 3312
EgeI GGCGCC 1 cut(s) 3395
EheI GGCGCC 1 cut(s) 3395
ErhI CCWWGG 4 cut(s) 910, 1822, 2358, 2794
Esp3I CGTCTC 1 cut(s) 274
FalI AAGNNNNNCTT 2 cut(s) 2808, 2840
FaqI GGGAC 8 cut(s) 656, 1221, 1300, 1516, 1966, 2955, 3002, 3107
FauI CCCGC 4 cut(s) 574, 874, 1277, 1421
FblI GTMKAC 1 cut(s) 2658
FokI GGATG 4 cut(s) 1349, 1637, 2690, 3392
FriOI GRGCYC 3 cut(s) 443, 606, 3312
FspBI CTAG 4 cut(s) 614, 1331, 1908, 3332
FspI TGCGCA 1 cut(s) 1167
GlaI GCGC 4 cut(s) 1167, 1329, 3370, 3395
GsaI CCCAGC 4 cut(s) 930, 938, 1667, 1854
GsuI CTGGAG 5 cut(s) 977, 1046, 1487, 2085, 3111
HaeII RGCGCY 2 cut(s) 1331, 3397
HgaI GACGC 3 cut(s) 184, 1010, 2343
HhaI GCGC 4 cut(s) 1168, 1330, 3371, 3396
Hin1I GRCGYC 4 cut(s) 195, 780, 3214, 3394
Hin6I GCGC 4 cut(s) 1166, 1328, 3369, 3394
HinP1I GCGC 4 cut(s) 1166, 1328, 3369, 3394
HincII GTYRAC 1 cut(s) 3468
HindII GTYRAC 1 cut(s) 3468
HindIII AAGCTT 3 cut(s) 875, 1160, 1598
Hpy166II GTNNAC 6 cut(s) 252, 2482, 2659, 2720, 2833, 3468
Hpy188I TCNGA 9 cut(s) 399, 1218, 1269, 1605, 2040, 2151, 2272, 3043, 3149
Hpy8I GTNNAC 6 cut(s) 252, 2482, 2659, 2720, 2833, 3468
Hpy99I CGWCG 4 cut(s) 782, 785, 1004, 1076
HpyCH4III ACNGT 8 cut(s) 265, 994, 1313, 1673, 2388, 2486, 2627, 2804
HpyCH4IV ACGT 5 cut(s) 780, 815, 1784, 3006, 3214
HpySE526I ACGT 5 cut(s) 780, 815, 1784, 3006, 3214
Hsp92I GRCGYC 4 cut(s) 195, 780, 3214, 3394
HspAI GCGC 4 cut(s) 1166, 1328, 3369, 3394
KasI GGCGCC 1 cut(s) 3393
Kpn2I TCCGGA 1 cut(s) 3449
LguI GCTCTTC 1 cut(s) 2073
Lsp1109I GCAGC 8 cut(s) 101, 378, 816, 1053, 2293, 2694, 3186, 3361
LweI GCATC 1 cut(s) 2412
MabI ACCWGGT 1 cut(s) 3112
MaeI CTAG 4 cut(s) 614, 1331, 1908, 3332
MaeII ACGT 5 cut(s) 780, 815, 1784, 3006, 3214
MaeIII GTNAC 4 cut(s) 1461, 1628, 1667, 3239
MbiI CCGCTC 1 cut(s) 2791
MfeI CAATTG 4 cut(s) 157, 1868, 2669, 2962
MflI RGATCY 7 cut(s) 388, 579, 1183, 1693, 2590, 3435, 3446
MhlI GDGCHC 7 cut(s) 443, 606, 1139, 2334, 2527, 3098, 3312
MlsI TGGCCA 1 cut(s) 1556
MluNI TGGCCA 1 cut(s) 1556
Mly113I GGCGCC 1 cut(s) 3394
MlyI GAGTC 4 cut(s) 95, 497, 956, 1024
MmeI TCCRAC 5 cut(s) 456, 612, 1218, 1671, 1954
Mox20I TGGCCA 1 cut(s) 1556
MroI TCCGGA 1 cut(s) 3449
MroXI GAANNNNTTC 3 cut(s) 698, 905, 3457
MscI TGGCCA 1 cut(s) 1556
MslI CAYNNNNRTG 5 cut(s) 1760, 2174, 2715, 3087, 3422
Msp20I TGGCCA 1 cut(s) 1556
MspA1I CMGCKG 3 cut(s) 1248, 1428, 3230
MspCI CTTAAG 2 cut(s) 878, 1619
MunI CAATTG 4 cut(s) 157, 1868, 2669, 2962
Mva1269I GAATGC 3 cut(s) 1998, 2142, 2967
MvaI CCWGG 8 cut(s) 1588, 1731, 1876, 2437, 2509, 3011, 3034, 3114
MvnI CGCG 1 cut(s) 1147
NarI GGCGCC 1 cut(s) 3394
NcoI CCATGG 1 cut(s) 1822
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 3 cut(s) 1461, 1667, 3239
NsbI TGCGCA 1 cut(s) 1167
NspI RCATGY 1 cut(s) 3275
PaeI GCATGC 1 cut(s) 3275
PaeR7I CTCGAG 5 cut(s) 671, 707, 1841, 2119, 3194
PagI TCATGA 2 cut(s) 2259, 3312
PciSI GCTCTTC 1 cut(s) 2073
PcsI WCGNNNNNNNCGW 2 cut(s) 747, 3012
PctI GAATGC 3 cut(s) 1998, 2142, 2967
PdmI GAANNNNTTC 3 cut(s) 698, 905, 3457
PfoI TCCNGGA 2 cut(s) 758, 896
PinAI ACCGGT 1 cut(s) 1205
PleI GAGTC 4 cut(s) 95, 497, 955, 1024
PluTI GGCGCC 1 cut(s) 3397
PpsI GAGTC 4 cut(s) 95, 497, 955, 1024
PpuMI RGGWCCY 3 cut(s) 1235, 2098, 3322
PshAI GACNNNNGTC 1 cut(s) 2353
Psp124BI GAGCTC 2 cut(s) 606, 3312
Psp5II RGGWCCY 3 cut(s) 1235, 2098, 3322
Psp6I CCWGG 8 cut(s) 1586, 1729, 1874, 2435, 2507, 3009, 3032, 3112
PspFI CCCAGC 4 cut(s) 926, 934, 1663, 1850
PspGI CCWGG 8 cut(s) 1586, 1729, 1874, 2435, 2507, 3009, 3032, 3112
PspPI GGNCC 8 cut(s) 335, 413, 563, 1235, 2047, 2098, 2665, 3322
PspPPI RGGWCCY 3 cut(s) 1235, 2098, 3322
PspXI VCTCGAGB 2 cut(s) 1841, 3194
PstI CTGCAG 2 cut(s) 91, 806
PstNI CAGNNNCTG 1 cut(s) 1447
PsuI RGATCY 7 cut(s) 388, 579, 1183, 1693, 2590, 3435, 3446
PvuII CAGCTG 2 cut(s) 1248, 3230
RsaI GTAC 6 cut(s) 916, 991, 1315, 2024, 2503, 2840
RsaNI GTAC 6 cut(s) 915, 990, 1314, 2023, 2502, 2839
RseI CAYNNNNRTG 5 cut(s) 1760, 2174, 2715, 3087, 3422
SacI GAGCTC 2 cut(s) 606, 3312
SapI GCTCTTC 1 cut(s) 2073
Sau96I GGNCC 8 cut(s) 335, 413, 563, 1235, 2047, 2098, 2665, 3322
SchI GAGTC 4 cut(s) 95, 497, 956, 1024
SduI GDGCHC 7 cut(s) 443, 606, 1139, 2334, 2527, 3098, 3312
SexAI ACCWGGT 1 cut(s) 3112
SfaNI GCATC 1 cut(s) 2412
SfcI CTRYAG 3 cut(s) 87, 802, 3219
SfoI GGCGCC 1 cut(s) 3395
Sfr274I CTCGAG 5 cut(s) 671, 707, 1841, 2119, 3194
SgrAI CRCCGGYG 1 cut(s) 1058
SinI GGWCC 3 cut(s) 1235, 2098, 3322
SlaI CTCGAG 5 cut(s) 671, 707, 1841, 2119, 3194
SmaI CCCGGG 2 cut(s) 215, 1240
SmiMI CAYNNNNRTG 5 cut(s) 1760, 2174, 2715, 3087, 3422
SpeI ACTAGT 1 cut(s) 613
SphI GCATGC 1 cut(s) 3275
SspDI GGCGCC 1 cut(s) 3393
SspMI CTAG 4 cut(s) 614, 1331, 1908, 3332
SstI GAGCTC 2 cut(s) 606, 3312
StyI CCWWGG 4 cut(s) 910, 1822, 2358, 2794
TaaI ACNGT 8 cut(s) 265, 994, 1313, 1673, 2388, 2486, 2627, 2804
TaiI ACGT 5 cut(s) 783, 818, 1787, 3009, 3217
TatI WGTACW 3 cut(s) 1313, 2022, 2838
TauI GCSGC 2 cut(s) 1147, 3399
TscAI CASTG 7 cut(s) 268, 1360, 1470, 2374, 2722, 2746, 3484
TseFI GTSAC 3 cut(s) 1461, 1667, 3239
TseI GCWGC 8 cut(s) 89, 366, 804, 1066, 2306, 2682, 3199, 3349
Tsp45I GTSAC 3 cut(s) 1461, 1667, 3239
TspDTI ATGAA 9 cut(s) 17, 930, 989, 2248, 2276, 2858, 2972, 3008, 3329
TspMI CCCGGG 2 cut(s) 213, 1238
TspRI CASTG 7 cut(s) 268, 1360, 1470, 2374, 2722, 2746, 3484
Vha464I CTTAAG 2 cut(s) 878, 1619
VpaK11BI GGWCC 3 cut(s) 1235, 2098, 3322
XapI RAATTY 3 cut(s) 1765, 2041, 2735
XceI RCATGY 1 cut(s) 3275
XcmI CCANNNNNNNNNTGG 1 cut(s) 2675
XhoI CTCGAG 5 cut(s) 671, 707, 1841, 2119, 3194
XmaI CCCGGG 2 cut(s) 213, 1238
XmiI GTMKAC 1 cut(s) 2658
XmnI GAANNNNTTC 3 cut(s) 698, 905, 3457
XspI CTAG 4 cut(s) 614, 1331, 1908, 3332
ZraI GACGTC 2 cut(s) 781, 3215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.