MD01G1014200.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012455
Physical Location & Seq
Reverse (-)
6677751 .. 6681397
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1014200.v1.1.491

Sequence Viewer

Length: 3600 bp
ATGAAACCCCACAAGTCCATTTCTCACTTCCCCTTCCCCTTCTTCCTCCTCCTCCTCCTTCCTCCGCCACCCACCTCTGCAACTCCGCCACCCACATCCTCCTCTTCTTACACAGACGCCCAGCAACTCCTCAGCTTCAAATCCTCCCTTCCAATCCCGACCCTCCTCCCCAACTGGCTCCCCAACCAAAACCCATGTTCCTTTTCTGGTATTTTCTGCAATGGAACAAGGGTTTCCTCCATACACCTCTCCTCCGTCTCCCTCGCCACAAATCTGACCGTTGTTTCCAACTTCCTCATGGCCCTTGACTCCCTTGAATCTCTCTCTCTCAAATCTGTTTCTCTCTCCGGCTCCATCTCCCTCCACTTCCCTCCCGGATCCAAGTGCAGCCCTCTCCTCACCTCCTTAGATCTGGCTCACAACTCCCTCTCCGGCCCTCTTTCCGACGTCCCCAACTTCGCCGCCGCTTGCTCCGCCTTGACCTTCCTCAACCTCTCCTCAAACTCTCTCGATTTCTCCACCATACCCTTCTCATCTGCCTTCCCCCTCCGCACCCTCCAGGTTCTCGATCTTTCTTACAACAAGATTACAGGCCCCAACGTCGTCCCTTGGATCTTATCCGACGGTTGCGGTAACTTGCAGAGCTTGGTTTTAAAAGGGAACAAAATCTCCGGCGAGATGAGCGTTGTCTCCACCTGCAAAAAGCTGGAGCACTTGGACTTGTCCTCCAACAACTTCTCCATTTCTCTTCCTTCTTTCGGCGATTGCTCGGCTTTGGACCACCTTGACATCTCTGGCAACAAGTTTTCTGGCGACGTTGGCCGCGCTATCTCCTCCTGCAAGCAGCTCACTTTCTTGAACCTCTCTATGAACCACTTCAACGGTCCGATTCCAGTCATGCCCACCAACAGTTTGAAGTTCCTGTCCCTTGGAGGTAATGGGTTTCAGGGTATAATTCCTATGAGCTTAATGGATTCGTGTGCAGAGCTTGTGGAGCTCGATCTATCGGCAAATAGCCTTTCGGGTTCGGTTCCTGATGCATTGAGCTCTTGTTCTTTGTTGGAGTCGCTGGACATATCCGGCAATAACTTTTCCAGTGAGTTGCCCGTTGAGATTTTGATGAAGCTCGCCAACTTAAAGGCTGTGTCGCTTTCTTTCAACAATTTCTATGGTACTCTGCCCGATTCTCTGTCTAAGCTCGCCACATTGGAGAGTTTGGATCTCAGCTCCAACAACTTTTCTGGGTCAATCCCAGCTGGGCTCTGCGGGGATCCTGGCAACATCTGGAAGGAGCTGTACCTTCAGAACAATCTGTTTACCGGCACGATTCCTCCGTCTCTGAGTAACTGTTCTCAGCTTGTTTCTCTTGATTTGAGCTTCAATTACCTCAAGGGCACCATCCCTTCGAGCTTGGGGTCATTGTCCAAGCTTCGCGATTTGATCATTTGGTTGAACCAGCTGAGTGGGGAAATCCCACAGGAGCTGATGTACCTTGGGTCACTTGAGAATCTCATTCTGGACTTCAACGAGCTTACAGGGTCGATTCCCATCGGTCTAAGCAACTGCACCAATTTGAATTGGATTTCATTGGCAAACAACAAGTTGAGTGGTGAGGTTCCCGGGTGGATTGGGAAGCTTCCAAATCTAGCAATACTCAAGCTCAGTAACAACTCATTCTTTGGTAGTATTCCCCCAGAGCTCGGTGACTGTAAGAGCTTGATATGGTTGGATCTCAATACCAACATGTTGAATGGCACAATCCCTCCTGCGCTTTTCAAGCAATCCGGAAACATTGCAGTGAATTTTGTTGCTTCCAAAACGTATGTTTATATCAAGAATGATGGTAGCAAGGAGTGCCATGGAGCAGGAAATTTGCTCGAGTTTGCGGGGATCAGAACTGAGCAGCTGAACAGGATTTCGACAAGAAATCCCTGCAACTTCACTAGAGTTTACAGAGGTATTCTCCAACCAACGTTTAACCATAACGGTTCTATGATTTTCCTTGACCTTTCGCATAACTCCTTGTCCGGTAGCATTCCCAAAGAGATTGGGAGCATGTACTATCTCTATATTTTGAATTTGGGCGATAACAATATATCTGGGTCAATCCCTCAAGAGCTTGGAAAAATGACCAGCCTTAACATTCTTGATCTCTCTAGCAATAGCCTCGCGGGGACTATTCCACCGGCTCTCAGTGGCCTTACCTTGCTAACGGAGATTGATCTTTCAAACAACCTTTTGTCTGGAACGATTCCAGAGTCAGGTCAGTTTGAGACATTCCCTGCCAACAGATTCGCGAACAATTCTGGCCTCTGTGGCTATCCGTTGGCTTCGTGTGGGGGGGCCTTGGGGCCAAGTGCAAATACACATCAAAAGTCCCATCGACGACAAGCATCCCTGGCAGGCAGTGTGGCAATGGGGTTACTCATCGCACTTTTCTGCATCTTTGGTTTGCTTATTGTTGCCATTGAAACCAAGAAAAGGCGAAAAAAGAAGGAAACAGCCCTTGATGTTTATATTGACAGTCGTAACCAATCAGGAACTGCCAATGGCTGGAAGCTAACTGGTGCCCGGGAAGCATTAAGCATCAACCTTTCGACATTTGAGAAGCCCCTTCAAAAGCTCACATTTGCAGATCTTCTCAAGGCCACCAATGGTTTCCACGATGACAGCCTTATTGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAGTTGAGAGATGGCAGCACTGTAGCCATAAAGAAACTAATACATATCAGCGGACAGGGTGATCGTGAATTCACTGCCGAAATGGAAACAATAGGGAAGATCAAGCACGGGAACCTTGTACCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTGGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACCGAAGAAGGCTGGCATCAAGTTAAACTGGGCTGCAAGGAGGAAGATTGCCATTGGGTCGGCGAGGGGACTGGCCTTTCTTCACCACAATTGTGTCCCACACATCATTCACAGGGATATGAAATCGAGCAACGTTCTGGTAGATGAAAATTTGGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTACAATGGACACCCATTTGAGTGTGAGCACTCTTGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGCTCCACGAAAGGCGATGTTTATAGTTATGGAGTAGTATTGCTTGAGCTGCTAACAGGAAAACGTCCTACAGATTCAGCTGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCCAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGTGAGGCTTGAAATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTCTGGACGATAGGCCATGGCGGCGTCCAACAATGATCCAAGTGATGGCAATGTTCAAGGAAATTCAAGCAGGGTCTGGGATGGACTCCCAATCAACCATAGCCACCGAGGACGGAGGTTTTGGCGTAGTTGAAATGGTAGAGATGAGCATAAAAGAAGTCGCCGAAAGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1200

Amino Acids

129.53

Weight (kDa)

6.14

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 38 - 74 1e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 212 - 269 1.7e-06 Leucine rich repeat
LRR_8 PF13855 235 - 293 5.5e-07 Leucine rich repeat
LRR_8 PF13855 304 - 364 3.4e-06 Leucine rich repeat
LRR_14 PF23598 346 - 580 7.3e-11 Leucine-rich repeat region
LRR_8 PF13855 500 - 558 4.5e-07 Leucine rich repeat
Island PF20141 596 - 660 1.3e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 689 - 747 1.2e-07 Leucine rich repeat
Pkinase PF00069 887 - 1157 3.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 891 - 1121 1.4e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 704
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 704
AccB1I GGYRCC 3 cut(s) 1394, 2567, 3155
AccB7I CCANNNNNTGG 2 cut(s) 2553, 3466
AccI GTMKAC 1 cut(s) 2703
AccII CGCG 4 cut(s) 825, 1434, 2171, 2297
AccIII TCCGGA 2 cut(s) 1784, 3352
AclI AACGTT 2 cut(s) 1973, 3054
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 6 cut(s) 1801, 1870, 2077, 2780, 3070, 3483
AcuI CTGAAG 1 cut(s) 1286
AcyI GRCGYC 3 cut(s) 117, 447, 3445
AfaI GTAC 7 cut(s) 1174, 1298, 1490, 2060, 2832, 2885, 3118
AfiI CCNNNNNNNGG 7 cut(s) 758, 1700, 2261, 2481, 2553, 2840, 3466
AflIII ACRYGT 1 cut(s) 1743
AjiI CACGTC 1 cut(s) 3373
AjnI CCWGG 4 cut(s) 558, 1273, 2397, 3160
AleI CACNNNNGTG 1 cut(s) 3012
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
Alw21I GWGCWC 6 cut(s) 714, 999, 1049, 1701, 3146, 3360
Alw26I GTCTC 5 cut(s) 262, 694, 1341, 2267, 3091
AlwNI CAGNNNCTG 3 cut(s) 1483, 2543, 3497
Ama87I CYCGRG 3 cut(s) 1619, 1877, 2571
Aor13HI TCCGGA 2 cut(s) 1784, 3352
ApeKI GCWGC 6 cut(s) 387, 844, 1903, 2727, 2954, 3247
ApoI RAATTY 6 cut(s) 1801, 1870, 2077, 2780, 3070, 3483
ArsI GACNNNNNNTTYG 2 cut(s) 3524, 3556
Asp700I GAANNNNTTC 1 cut(s) 875
AspLEI GCGC 2 cut(s) 827, 1771
AspS9I GGNCC 8 cut(s) 301, 434, 593, 778, 884, 2343, 2351, 2710
AsuC2I CCSGG 5 cut(s) 375, 1620, 1621, 2572, 2573
AsuHPI GGTGA 6 cut(s) 391, 1622, 1715, 2708, 2783, 2996
AvaI CYCGRG 3 cut(s) 1619, 1877, 2571
AvaII GGWCC 2 cut(s) 778, 884
BaeGI GKGCMC 2 cut(s) 1397, 2572
BamHI GGATCC 2 cut(s) 377, 1270
BanI GGYRCC 3 cut(s) 1394, 2567, 3155
BanII GRGCYC 5 cut(s) 999, 1049, 1263, 1701, 3360
BarI GAAGNNNNNNTAC 2 cut(s) 1280, 1312
BauI CACGAG 1 cut(s) 3299
Bbv12I GWGCWC 6 cut(s) 714, 999, 1049, 1701, 3146, 3360
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 6 cut(s) 399, 856, 1915, 2739, 2941, 3234
BccI CCATC 8 cut(s) 362, 1406, 1556, 1835, 2388, 2717, 3460, 3496
BceAI ACGGC 1 cut(s) 2882
BcgI CGANNNNNNTGC 2 cut(s) 2149, 2183
BciT130I CCWGG 4 cut(s) 560, 1275, 2399, 3162
BciVI GTATCC 1 cut(s) 2837
BclI TGATCA 1 cut(s) 1440
BcnI CCSGG 5 cut(s) 375, 1620, 1621, 2572, 2573
BcoDI GTCTC 5 cut(s) 262, 694, 1341, 2267, 3091
BfaI CTAG 3 cut(s) 1646, 1944, 2157
BfmI CTRYAG 2 cut(s) 2733, 3267
BfuAI ACCTGC 1 cut(s) 704
BfuI GTATCC 1 cut(s) 2837
BglI GCCNNNNNGGC 3 cut(s) 2316, 2679, 3442
BglII AGATCT 2 cut(s) 409, 2635
Bme1390I CCNGG 9 cut(s) 375, 560, 1275, 1620, 1621, 2399, 2572, 2573, 3162
Bme18I GGWCC 2 cut(s) 778, 884
BmeT110I CYCGRG 3 cut(s) 1619, 1877, 2571
BmgBI CACGTC 1 cut(s) 3373
BmgT120I GGNCC 8 cut(s) 301, 434, 593, 778, 884, 2343, 2351, 2710
BmrFI CCNGG 9 cut(s) 375, 560, 1275, 1620, 1621, 2399, 2572, 2573, 3162
BmrI ACTGGG 2 cut(s) 2707, 2959
BmsI GCATC 6 cut(s) 1027, 2402, 2451, 2595, 2946, 3188
BmuI ACTGGG 2 cut(s) 2707, 2959
BplI GAGNNNNNCTC 2 cut(s) 1947, 1979
BpmI CTGGAG 2 cut(s) 542, 728
Bpu10I CCTNAGC 1 cut(s) 131
BpuEI CTTGAG 6 cut(s) 1373, 1523, 1640, 2097, 2627, 3263
BpuMI CCSGG 5 cut(s) 375, 1620, 1621, 2572, 2573
BsaBI GATNNNNATC 1 cut(s) 1547
BsaHI GRCGYC 3 cut(s) 117, 447, 3445
BsaWI WCCGGW 3 cut(s) 1784, 2027, 3352
Bsc4I CCNNNNNNNGG 7 cut(s) 758, 1700, 2261, 2481, 2553, 2840, 3466
Bse118I RCCGGY 2 cut(s) 1319, 2185
Bse1I ACTGG 7 cut(s) 179, 893, 1095, 2569, 2713, 2954, 2997
Bse3DI GCAATG 4 cut(s) 226, 1791, 2421, 3477
Bse8I GATNNNNATC 1 cut(s) 1547
BseAI TCCGGA 2 cut(s) 1784, 3352
BseBI CCWGG 4 cut(s) 560, 1275, 2399, 3162
BseGI GGATG 4 cut(s) 95, 1398, 2393, 3507
BseJI GATNNNNATC 1 cut(s) 1547
BseLI CCNNNNNNNGG 7 cut(s) 758, 1700, 2261, 2481, 2553, 2840, 3466
BseMI GCAATG 4 cut(s) 226, 1791, 2421, 3477
BseMII CTCAG 8 cut(s) 145, 1237, 1331, 1367, 1451, 1675, 1890, 2206
BseNI ACTGG 7 cut(s) 179, 893, 1095, 2569, 2713, 2954, 2997
BseSI GKGCMC 2 cut(s) 1397, 2572
BseXI GCAGC 6 cut(s) 399, 856, 1915, 2739, 2941, 3234
BseYI CCCAGC 3 cut(s) 120, 1252, 1256
BsgI GTGCAG 3 cut(s) 406, 1002, 1549
Bsh1236I CGCG 4 cut(s) 825, 1434, 2171, 2297
BshNI GGYRCC 3 cut(s) 1394, 2567, 3155
BsiHKAI GWGCWC 6 cut(s) 714, 999, 1049, 1701, 3146, 3360
BsiHKCI CYCGRG 3 cut(s) 1619, 1877, 2571
BslFI GGGAC 8 cut(s) 434, 590, 910, 2188, 2362, 3002, 3003, 3155
BslI CCNNNNNNNGG 7 cut(s) 758, 1700, 2261, 2481, 2553, 2840, 3466
BsmAI GTCTC 5 cut(s) 262, 694, 1341, 2267, 3091
BsmBI CGTCTC 2 cut(s) 262, 1341
BsmFI GGGAC 8 cut(s) 434, 590, 910, 2188, 2362, 3002, 3003, 3155
BsmI GAATGC 1 cut(s) 2034
BsoBI CYCGRG 3 cut(s) 1619, 1877, 2571
Bsp1286I GDGCHC 9 cut(s) 714, 999, 1049, 1263, 1397, 1701, 2572, 3146, 3360
Bsp13I TCCGGA 2 cut(s) 1784, 3352
Bsp19I CCATGG 2 cut(s) 1858, 3437
Bsp68I TCGCGA 2 cut(s) 1434, 2297
BspCNI CTCAG 8 cut(s) 144, 1236, 1332, 1366, 1452, 1674, 1891, 2205
BspEI TCCGGA 2 cut(s) 1784, 3352
BspFNI CGCG 4 cut(s) 825, 1434, 2171, 2297
BspHI TCATGA 1 cut(s) 3360
BspMI ACCTGC 1 cut(s) 704
BspT107I GGYRCC 3 cut(s) 1394, 2567, 3155
BsrDI GCAATG 4 cut(s) 226, 1791, 2421, 3477
BsrFI RCCGGY 2 cut(s) 1319, 2185
BsrI ACTGG 7 cut(s) 179, 893, 1095, 2569, 2713, 2954, 2997
BssAI RCCGGY 2 cut(s) 1319, 2185
BssNAI GTATAC 1 cut(s) 2704
BssNI GRCGYC 3 cut(s) 117, 447, 3445
BssSI CACGAG 1 cut(s) 3299
BssT1I CCWWGG 7 cut(s) 608, 928, 1492, 1858, 2346, 2839, 3437
Bst1107I GTATAC 1 cut(s) 2704
Bst2BI CACGAG 1 cut(s) 3299
Bst2UI CCWGG 4 cut(s) 560, 1275, 2399, 3162
Bst4CI ACNGT 9 cut(s) 280, 626, 884, 911, 1349, 1709, 1988, 2525, 2734
Bst6I CTCTTC 2 cut(s) 109, 753
BstACI GRCGYC 3 cut(s) 117, 447, 3445
BstAPI GCANNNNNTGC 1 cut(s) 1854
BstC8I GCNNGC 8 cut(s) 469, 842, 1128, 1200, 2404, 2935, 3154, 3593
BstDSI CCRYGG 2 cut(s) 1858, 3437
BstF5I GGATG 4 cut(s) 95, 1398, 2393, 3507
BstFNI CGCG 4 cut(s) 825, 1434, 2171, 2297
BstHHI GCGC 2 cut(s) 827, 1771
BstMAI GTCTC 5 cut(s) 262, 694, 1341, 2267, 3091
BstNI CCWGG 4 cut(s) 560, 1275, 2399, 3162
BstNSI RCATGY 2 cut(s) 1747, 2059
BstSCI CCNGG 9 cut(s) 373, 558, 1273, 1618, 1619, 2397, 2570, 2571, 3160
BstSFI CTRYAG 2 cut(s) 2733, 3267
BstSLI GKGCMC 2 cut(s) 1397, 2572
BstUI CGCG 4 cut(s) 825, 1434, 2171, 2297
BstV1I GCAGC 6 cut(s) 399, 856, 1915, 2739, 2941, 3234
BstX2I RGATCY 7 cut(s) 377, 409, 612, 1219, 1270, 1729, 2635
BstXI CCANNNNNNTGG 1 cut(s) 1463
BstYI RGATCY 7 cut(s) 377, 409, 612, 1219, 1270, 1729, 2635
BstZ17I GTATAC 1 cut(s) 2704
BsuI GTATCC 1 cut(s) 2837
BtgI CCRYGG 2 cut(s) 1858, 3437
BtgZI GCGATG 2 cut(s) 2413, 3228
BtrI CACGTC 1 cut(s) 3373
BtsCI GGATG 4 cut(s) 95, 1398, 2393, 3507
BtsI GCAGTG 3 cut(s) 1803, 2413, 2784
BtsIMutI CAGTG 6 cut(s) 1102, 1803, 2200, 2413, 2730, 2784
BtuMI TCGCGA 2 cut(s) 1434, 2297
BveI ACCTGC 1 cut(s) 704
Cac8I GCNNGC 8 cut(s) 469, 842, 1128, 1200, 2404, 2935, 3154, 3593
CaiI CAGNNNCTG 3 cut(s) 1483, 2543, 3497
CciI TCATGA 1 cut(s) 3360
CfoI GCGC 2 cut(s) 827, 1771
Cfr10I RCCGGY 2 cut(s) 1319, 2185
Cfr13I GGNCC 8 cut(s) 301, 434, 593, 778, 884, 2343, 2351, 2710
Cfr9I CCCGGG 2 cut(s) 1619, 2571
CpoI CGGWCCG 1 cut(s) 884
CseI GACGC 2 cut(s) 125, 3434
Csp6I GTAC 7 cut(s) 1173, 1297, 1489, 2059, 2831, 2884, 3117
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CspI CGGWCCG 1 cut(s) 884
CviQI GTAC 7 cut(s) 1173, 1297, 1489, 2059, 2831, 2884, 3117
DraI TTTAAA 1 cut(s) 654
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 2 cut(s) 109, 753
EarI CTCTTC 2 cut(s) 109, 753
EciI GGCGGA 3 cut(s) 54, 75, 463
Ecl136II GAGCTC 4 cut(s) 997, 1047, 1699, 3358
Eco130I CCWWGG 7 cut(s) 608, 928, 1492, 1858, 2346, 2839, 3437
Eco24I GRGCYC 5 cut(s) 999, 1049, 1263, 1701, 3360
Eco47I GGWCC 2 cut(s) 778, 884
Eco53kI GAGCTC 4 cut(s) 997, 1047, 1699, 3358
Eco57I CTGAAG 1 cut(s) 1286
Eco88I CYCGRG 3 cut(s) 1619, 1877, 2571
EcoICRI GAGCTC 4 cut(s) 997, 1047, 1699, 3358
EcoO109I RGGNCCY 2 cut(s) 593, 2343
EcoRI GAATTC 1 cut(s) 2780
EcoRII CCWGG 4 cut(s) 558, 1273, 2397, 3160
EcoT14I CCWWGG 7 cut(s) 608, 928, 1492, 1858, 2346, 2839, 3437
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 5 cut(s) 999, 1049, 1263, 1701, 3360
ErhI CCWWGG 7 cut(s) 608, 928, 1492, 1858, 2346, 2839, 3437
Esp3I CGTCTC 2 cut(s) 262, 1341
FalI AAGNNNNNCTT 2 cut(s) 2853, 2885
FaqI GGGAC 8 cut(s) 434, 590, 910, 2188, 2362, 3002, 3003, 3155
FauI CCCGC 3 cut(s) 1259, 1879, 2164
FbaI TGATCA 1 cut(s) 1440
FblI GTMKAC 1 cut(s) 2703
FokI GGATG 4 cut(s) 82, 1385, 2380, 3514
FriOI GRGCYC 5 cut(s) 999, 1049, 1263, 1701, 3360
FspBI CTAG 3 cut(s) 1646, 1944, 2157
GlaI GCGC 2 cut(s) 826, 1770
GsaI CCCAGC 3 cut(s) 124, 1256, 1260
GsuI CTGGAG 2 cut(s) 542, 728
HgaI GACGC 2 cut(s) 125, 3434
HhaI GCGC 2 cut(s) 827, 1771
Hin1I GRCGYC 3 cut(s) 117, 447, 3445
Hin6I GCGC 2 cut(s) 825, 1769
HinP1I GCGC 2 cut(s) 825, 1769
HindIII AAGCTT 3 cut(s) 1427, 1634, 2899
HphI GGTGA 6 cut(s) 391, 1622, 1715, 2708, 2783, 2996
Hpy166II GTNNAC 3 cut(s) 1317, 1951, 2704
Hpy188I TCNGA 8 cut(s) 276, 445, 622, 888, 1305, 1341, 1895, 3091
Hpy8I GTNNAC 3 cut(s) 1317, 1951, 2704
Hpy99I CGWCG 5 cut(s) 449, 605, 626, 818, 2388
HpyCH4III ACNGT 9 cut(s) 280, 626, 884, 911, 1349, 1709, 1988, 2525, 2734
HpyCH4IV ACGT 8 cut(s) 447, 600, 816, 1820, 1973, 3054, 3262, 3372
HpySE526I ACGT 8 cut(s) 447, 600, 816, 1820, 1973, 3054, 3262, 3372
Hsp92I GRCGYC 3 cut(s) 117, 447, 3445
HspAI GCGC 2 cut(s) 825, 1769
Kpn2I TCCGGA 2 cut(s) 1784, 3352
Ksp22I TGATCA 1 cut(s) 1440
Lsp1109I GCAGC 6 cut(s) 399, 856, 1915, 2739, 2941, 3234
LweI GCATC 6 cut(s) 1027, 2402, 2451, 2595, 2946, 3188
MaeI CTAG 3 cut(s) 1646, 1944, 2157
MaeII ACGT 8 cut(s) 447, 600, 816, 1820, 1973, 3054, 3262, 3372
MaeIII GTNAC 7 cut(s) 632, 1343, 1497, 1664, 1703, 2421, 2528
MboII GAAGA 9 cut(s) 34, 96, 740, 2630, 2821, 2873, 2938, 2977, 2993
MfeI CAATTG 1 cut(s) 3010
MflI RGATCY 7 cut(s) 377, 409, 612, 1219, 1270, 1729, 2635
MhlI GDGCHC 9 cut(s) 714, 999, 1049, 1263, 1397, 1701, 2572, 3146, 3360
MlyI GAGTC 5 cut(s) 302, 1073, 2267, 3093, 3500
MmeI TCCRAC 9 cut(s) 312, 468, 645, 753, 1041, 1254, 1707, 1990, 3473
Mph1103I ATGCAT 1 cut(s) 1042
MroI TCCGGA 2 cut(s) 1784, 3352
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 7 cut(s) 653, 968, 1136, 1977, 2139, 2582, 2945
MslI CAYNNNNRTG 3 cut(s) 1796, 3012, 3135
MspA1I CMGCKG 5 cut(s) 1256, 1459, 1906, 2763, 3278
MspR9I CCNGG 9 cut(s) 375, 560, 1275, 1620, 1621, 2399, 2572, 2573, 3162
MunI CAATTG 1 cut(s) 3010
Mva1269I GAATGC 1 cut(s) 2034
MvaI CCWGG 4 cut(s) 560, 1275, 2399, 3162
MvnI CGCG 4 cut(s) 825, 1434, 2171, 2297
NciI CCSGG 5 cut(s) 375, 1620, 1621, 2572, 2573
NcoI CCATGG 2 cut(s) 1858, 3437
NmeAIII GCCGAG 1 cut(s) 749
NmuCI GTSAC 2 cut(s) 1497, 1703
NruI TCGCGA 2 cut(s) 1434, 2297
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 1747, 2059
OliI CACNNNNGTG 1 cut(s) 3012
PaeR7I CTCGAG 1 cut(s) 1877
PagI TCATGA 1 cut(s) 3360
PaqCI CACCTGC 1 cut(s) 704
PciI ACATGT 1 cut(s) 1743
PcsI WCGNNNNNNNCGW 1 cut(s) 1331
PctI GAATGC 1 cut(s) 2034
PdmI GAANNNNTTC 1 cut(s) 875
PflMI CCANNNNNTGG 2 cut(s) 2553, 3466
PfoI TCCNGGA 1 cut(s) 373
PleI GAGTC 5 cut(s) 302, 1072, 2266, 3092, 3500
PpsI GAGTC 5 cut(s) 302, 1072, 2266, 3092, 3500
PscI ACATGT 1 cut(s) 1743
Psp124BI GAGCTC 4 cut(s) 999, 1049, 1701, 3360
Psp1406I AACGTT 2 cut(s) 1973, 3054
Psp6I CCWGG 4 cut(s) 558, 1273, 2397, 3160
PspFI CCCAGC 3 cut(s) 120, 1252, 1256
PspGI CCWGG 4 cut(s) 558, 1273, 2397, 3160
PspPI GGNCC 8 cut(s) 301, 434, 593, 778, 884, 2343, 2351, 2710
PspXI VCTCGAGB 1 cut(s) 1877
PstNI CAGNNNCTG 3 cut(s) 1483, 2543, 3497
PsuI RGATCY 7 cut(s) 377, 409, 612, 1219, 1270, 1729, 2635
PvuII CAGCTG 4 cut(s) 1256, 1459, 1906, 3278
RruI TCGCGA 2 cut(s) 1434, 2297
RsaI GTAC 7 cut(s) 1174, 1298, 1490, 2060, 2832, 2885, 3118
RsaNI GTAC 7 cut(s) 1173, 1297, 1489, 2059, 2831, 2884, 3117
RseI CAYNNNNRTG 3 cut(s) 1796, 3012, 3135
Rsr2I CGGWCCG 1 cut(s) 884
RsrII CGGWCCG 1 cut(s) 884
SacI GAGCTC 4 cut(s) 999, 1049, 1701, 3360
SaqAI TTAA 7 cut(s) 653, 968, 1136, 1977, 2139, 2582, 2945
Sau96I GGNCC 8 cut(s) 301, 434, 593, 778, 884, 2343, 2351, 2710
SchI GAGTC 5 cut(s) 302, 1073, 2267, 3093, 3500
ScrFI CCNGG 9 cut(s) 375, 560, 1275, 1620, 1621, 2399, 2572, 2573, 3162
SduI GDGCHC 9 cut(s) 714, 999, 1049, 1263, 1397, 1701, 2572, 3146, 3360
SfaNI GCATC 6 cut(s) 1027, 2402, 2451, 2595, 2946, 3188
SfcI CTRYAG 2 cut(s) 2733, 3267
Sfr274I CTCGAG 1 cut(s) 1877
SinI GGWCC 2 cut(s) 778, 884
SlaI CTCGAG 1 cut(s) 1877
SmaI CCCGGG 2 cut(s) 1621, 2573
SmiMI CAYNNNNRTG 3 cut(s) 1796, 3012, 3135
SmlI CTYRAG 7 cut(s) 1388, 1502, 1655, 1877, 2112, 2642, 3242
SmoI CTYRAG 7 cut(s) 1388, 1502, 1655, 1877, 2112, 2642, 3242
SspMI CTAG 3 cut(s) 1646, 1944, 2157
SstI GAGCTC 4 cut(s) 999, 1049, 1701, 3360
StyD4I CCNGG 9 cut(s) 373, 558, 1273, 1618, 1619, 2397, 2570, 2571, 3160
StyI CCWWGG 7 cut(s) 608, 928, 1492, 1858, 2346, 2839, 3437
TaaI ACNGT 9 cut(s) 280, 626, 884, 911, 1349, 1709, 1988, 2525, 2734
TaiI ACGT 8 cut(s) 450, 603, 819, 1823, 1976, 3057, 3265, 3375
TaqII GACCGA 1 cut(s) 1541
TatI WGTACW 3 cut(s) 2058, 2883, 3116
TauI GCSGC 4 cut(s) 464, 467, 825, 3445
Tru1I TTAA 7 cut(s) 653, 968, 1136, 1977, 2139, 2582, 2945
Tru9I TTAA 7 cut(s) 653, 968, 1136, 1977, 2139, 2582, 2945
TscAI CASTG 6 cut(s) 1102, 1803, 2200, 2413, 2737, 2791
TseFI GTSAC 2 cut(s) 1497, 1703
TseI GCWGC 6 cut(s) 387, 844, 1903, 2727, 2954, 3247
Tsp45I GTSAC 2 cut(s) 1497, 1703
TspDTI ATGAA 9 cut(s) 17, 884, 1136, 1575, 2903, 2934, 3056, 3081, 3377
TspGWI ACGGA 5 cut(s) 244, 1323, 2228, 2313, 3549
TspMI CCCGGG 2 cut(s) 1619, 2571
TspRI CASTG 6 cut(s) 1102, 1803, 2200, 2413, 2737, 2791
Van91I CCANNNNNTGG 2 cut(s) 2553, 3466
VpaK11BI GGWCC 2 cut(s) 778, 884
XapI RAATTY 6 cut(s) 1801, 1870, 2077, 2780, 3070, 3483
XceI RCATGY 2 cut(s) 1747, 2059
XcmI CCANNNNNNNNNTGG 2 cut(s) 295, 2720
XhoI CTCGAG 1 cut(s) 1877
XmaI CCCGGG 2 cut(s) 1619, 2571
XmiI GTMKAC 1 cut(s) 2703
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 3 cut(s) 1646, 1944, 2157
ZraI GACGTC 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.