pycom15g28930
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012455
Physical Location & Seq
Reverse (-)
25406069 .. 25409332
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g28930.1

Sequence Viewer

Length: 3264 bp
ATGACCCTCGACTCCCTTGAATCTCTCTTTCTCAATTCAGCCTCTCTCTCCGGCTCTATCTCCCTCCACTTCCCTACCAGAACCAAGTGCAGCCCTCACCTCACCTCCTTAGATCTGGCTCACAACTCCCTTTCCGGCCCTCTTTCAGACGTCCCCGACTTCGCCGCCGCTTGCTCCGCCTTGACCTTCCTCAACCTCTCTTCAAACTCTCTCGTTTTACCCACCAAACCGTCCTCCTCCGCCTTTCCCCTCCGCAACCTCCAAGTTCTTGATCTTTCTTACAACAAGATTACAGGCCCCAACGTCGTCCGTTGGATCTTATCCGACGGTTGCGGTGACTTGCAGAGGTTGGTTTTGAAGGGGAACAAAATCTCCGGCGAGATGAGCGCTGTGTCCACCTGCAGCAAGCTGGAGCACTTGGACTTGTCCTCCAACAACTTCTCCATTTCTCTTCCGTCTTTCGGGGATTGCTCGGCTTTGGACCACCTTGACATCTCTGGCAACAAGTTTTCTGGCGACGTTGGTCGCGCTATCTCCTCCTGCAAGCAGCTCAGTTTCTTGAACCTCTCCATGAACCACTTCGACGGTCCGATTCCGGCCATGCCCACCAACAGCTTGAAGTTCCTGTCACTTGGAGATAATAGGTTTCAGGGTATAATTCCTGTGAGCTTAATGGATTCGTGTGCAGAGCTTGTGGAGCTCGATCTGTCGGCTAATAGCCTTTCGGGTTCGGTTCCTGATGCACTGAGCTCTTGTTCTTCGTTGGAATCGCTGGACATATCCGCCAATAACTTTTCTGGTGAGTTGCCCGTTGAGATTTTGATGAAGCTCACCAACTTAAAGGCTGTGTCGCTTTCTTTCAACAAATTCTTTGGTCCTCTGCCCAATTCTCTGTCTAAGCTCGCGACATTGGAGAGCTTGGATCTCAGCTCCAACAGTTTGTCTGGGTCAATCCCAGCTGGGCTCTGCGGGGAGCCTAGCAACAGCTGGAAGGAGCTGTACCTTCAGAACAATCTGTTTACCGGCACGATCCCTCCGTCTTTGAGCAACTGTTCTCAGCTTGTTTCTCTTGATTTGAGCTTCAATAATCTCAAGGGCACCATCCCTTCAAGCTTGGGGTCGTTGTCAAAGCTTCGCGATTTGATCATTTGGTTGAACCAGCTGAGTGGGGAAATCCCACAAGAGCTGATGTACCTTGGGTCGCTTGAGAATCTCATTCTGGACTTCAACGACCTTACGGGGTCGATTCCCATCGGTTTAAGCAACTGCACCAATTTGAATTGGATTTCATTGGCTAACAACAAGTTGAGTGGTGAGGTTCCCAGGTGGATTGGGAAGCTTCCAAATCTAGCAATACTCAAGCTCAGTAACAACTCATTCTCTGGTGATATTCCCCCGGAGCTCGGTGACTGTAAGAGCTTGATATGGTTGGATCTCAATACCAACTTGTTGAATGGCACAATCCCTCCTTCGCTTTTCAAGCAATCCGGAAACATCGCAGTGAATTTTGTTGCTTCCAAAACGTATGTATATATCAAGAATGATGGTAGCAAGGAGTGCCATGGAGCAGGAAATTTGCTCGAGTTTGCAGGTATCAGAGACGGGCAACTGAACAGGATTTCGACAAGAAATCCCTGCAACTTCACTAGAGTTTACAGAGGTATTCTCCAACCAACGTTTAACCATAATGGTTCTATGATTTTCCTTGATCGTTCGCATAATTCCTTGTCCGGTAGCATTCCCAAAGAGATTGGGAGCATGTACTATCTCTATATTTTGAATTTGGGCCATAACAATATATCTGGTTCAATCCCACAAGAGCTTGGAAAAATGACCAGCCTTAACATTCTTGATCTCTCTAGCAATAGCCTTGCGGGGACTATTCCACCGGCTCTGAGTGGCCTTACCTTGCTAACGGAAAAGATCGATCTATCAAACAACCGTTTGTCTGGAATGATTCCAGAGTCGGGTCAATTTGAGACGTTCCCTGCCTACAGATTTGCCAACAATTCTGGCCTATGTGGCTATCCTTTGGCTTCATGTGGGGGAGCTTTGGGGCCAAATGCAAATGCACATCAAAAGTCTCATCGAAGAGAACCATCCCTGGTGGGCAGTGTGGCAATGGGGTTACTCATCTCACTTTTCTGCATCTTTGGTTTGTTCATTGTTGCCATTGAAACCAAGAAAAGGCGAAAAAAAAAGGAATCGGCCCTTGATGTTTGTATCGACAGCCGTAACCAATCAGGAACTGCCAATGGCTGGAAGCTAACAGGTGCCCGGGAAGCATTAAGCATCAACCTTGCGACATTTGAGAAGCCCCTTCAAAAGCTCACTTTTGCAGATCTTCTCGAGGCCACCAATGGTTTCCACGATAACAGCCTTATTGGCAAAGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAAGATGGCAGTGTCGTAGCCATAAAGAAACTAATACATATCAGCGGACAGGGTGATCGCGAATTCACTGCAGAAATGGAAACAATAGGGAAGATCAAGCACCGGAACCTTGTCCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTGGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACCGAAGAAAGCTGGCATCAAGTTGAACTGGGCTGCAAGGAGGAAGATTGCCATTGGGTCCGCGAGGGGACTGGCCTTTCTTCACCACAATTGCATCCCACACATCATTCACAGGGATATGAAATCAAGCAACGTGCTGGTGGATGAAAATTTGGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACCCATTTGAGTGTGAGCACTCTCGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGTTCCACGAAAGGTGATGTTTATAGTTATGGAGTAGTATTGCTTGAGCTGCTAACGGGAAGACGGCCTACAGATTCAGCAGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCTAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGCAAGCCTTGAGATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGACGACAGGCCATGGCGGCGTCCAACAATCGATCCAAGTGATGGCAATGTTCAAGGAAATCCAAGCCGGGTCTGGGATGGACTCCCAATCAACGATAGCCATGGACGACGACGGAGTTTTTGGCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1088

Amino Acids

118.04

Weight (kDa)

6.78

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 114 - 170 2.8e-06 Leucine rich repeat
LRR_8 PF13855 136 - 194 1.3e-06 Leucine rich repeat
LRR_14 PF23598 150 - 388 1.1e-09 Leucine-rich repeat region
LRR_8 PF13855 205 - 265 1.7e-07 Leucine rich repeat
LRR_8 PF13855 401 - 459 1.8e-06 Leucine rich repeat
LRR_8 PF13855 425 - 484 3.8e-06 Leucine rich repeat
Island PF20141 497 - 561 7e-31 Brassinosteroid receptor island domain
LRR_8 PF13855 590 - 649 9.7e-06 Leucine rich repeat
Pkinase PF00069 789 - 1054 5.7e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 793 - 1022 2.9e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 2 cut(s) 407, 1580
AccB1I GGYRCC 3 cut(s) 1097, 2273, 2861
AccB7I CCANNNNNTGG 2 cut(s) 2259, 3173
AccI GTMKAC 1 cut(s) 2409
AccII CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
AccIII TCCGGA 2 cut(s) 1487, 3058
AclI AACGTT 1 cut(s) 1676
AclWI GGATC 6 cut(s) 323, 930, 1024, 1440, 3050, 3158
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 6 cut(s) 866, 1504, 1573, 1780, 2486, 2776
AcuI CTGAAG 1 cut(s) 989
AcyI GRCGYC 2 cut(s) 150, 3151
AfaI GTAC 4 cut(s) 1001, 1193, 1763, 2591
AfeI AGCGCT 1 cut(s) 388
AfiI CCNNNNNNNGG 8 cut(s) 461, 1403, 1967, 2187, 2259, 2546, 3173, 3205
AjnI CCWGG 3 cut(s) 1322, 2103, 2866
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
Alw21I GWGCWC 6 cut(s) 417, 702, 752, 1404, 2852, 3066
Alw26I GTCTC 4 cut(s) 1593, 1973, 2088, 2797
AlwI GGATC 6 cut(s) 323, 930, 1024, 1440, 3050, 3158
AlwNI CAGNNNCTG 1 cut(s) 2249
Ama87I CYCGRG 3 cut(s) 1580, 2277, 2348
Aor13HI TCCGGA 2 cut(s) 1487, 3058
Aor51HI AGCGCT 1 cut(s) 388
ApeKI GCWGC 5 cut(s) 90, 402, 547, 2660, 2953
ApoI RAATTY 6 cut(s) 866, 1504, 1573, 1780, 2486, 2776
ArsI GACNNNNNNTTYG 1 cut(s) 3234
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 389, 530
AsuC2I CCSGG 4 cut(s) 1397, 2278, 2279, 3200
AvaI CYCGRG 3 cut(s) 1580, 2277, 2348
AvaII GGWCC 4 cut(s) 481, 587, 875, 2685
BaeGI GKGCMC 2 cut(s) 1100, 2278
BanI GGYRCC 3 cut(s) 1097, 2273, 2861
BanII GRGCYC 5 cut(s) 702, 752, 966, 1404, 3066
BarI GAAGNNNNNNTAC 4 cut(s) 983, 1015, 2956, 2988
BauI CACGAG 1 cut(s) 3005
BbsI GAAGAC 1 cut(s) 2971
Bbv12I GWGCWC 6 cut(s) 417, 702, 752, 1404, 2852, 3066
BbvI GCAGC 5 cut(s) 102, 414, 559, 2647, 2940
BccI CCATC 7 cut(s) 1109, 1259, 1538, 2107, 2423, 3167, 3203
BceAI ACGGC 3 cut(s) 2217, 2588, 2984
BcgI CGANNNNNNTGC 2 cut(s) 2474, 2508
BciT130I CCWGG 3 cut(s) 1324, 2105, 2868
BciVI GTATCC 1 cut(s) 2543
BclI TGATCA 1 cut(s) 1143
BcnI CCSGG 4 cut(s) 1397, 2278, 2279, 3200
BcoDI GTCTC 4 cut(s) 1593, 1973, 2088, 2797
BfaI CTAG 4 cut(s) 978, 1349, 1647, 1860
BfmI CTRYAG 4 cut(s) 400, 1993, 2493, 2973
BfoI RGCGCY 1 cut(s) 390
BfuAI ACCTGC 2 cut(s) 407, 1580
BfuI GTATCC 1 cut(s) 2543
BglI GCCNNNNNGGC 3 cut(s) 2022, 2385, 3148
BglII AGATCT 2 cut(s) 112, 2341
BisI GCNGC 8 cut(s) 91, 165, 168, 403, 548, 2661, 2954, 3149
BlsI GCNGC 8 cut(s) 92, 166, 169, 404, 549, 2662, 2955, 3150
Bme1390I CCNGG 7 cut(s) 1324, 1397, 2105, 2278, 2279, 2868, 3200
Bme18I GGWCC 4 cut(s) 481, 587, 875, 2685
BmeT110I CYCGRG 3 cut(s) 1580, 2277, 2348
BmrFI CCNGG 7 cut(s) 1324, 1397, 2105, 2278, 2279, 2868, 3200
BmrI ACTGGG 1 cut(s) 2665
BmsI GCATC 5 cut(s) 730, 2157, 2301, 2652, 2730
BmuI ACTGGG 1 cut(s) 2665
BoxI GACNNNNGTC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 2971
BplI GAGNNNNNCTC 2 cut(s) 1650, 1682
BpmI CTGGAG 1 cut(s) 431
BpuEI CTTGAG 5 cut(s) 1076, 1226, 1343, 2969, 3107
BpuMI CCSGG 4 cut(s) 1397, 2278, 2279, 3200
Bsa29I ATCGAT 2 cut(s) 1926, 3162
BsaBI GATNNNNATC 1 cut(s) 1250
BsaHI GRCGYC 2 cut(s) 150, 3151
BsaWI WCCGGW 4 cut(s) 1487, 1730, 2526, 3058
Bsc4I CCNNNNNNNGG 8 cut(s) 461, 1403, 1967, 2187, 2259, 2546, 3173, 3205
Bse118I RCCGGY 2 cut(s) 1022, 1888
Bse1I ACTGG 2 cut(s) 2660, 2703
Bse3DI GCAATG 3 cut(s) 2127, 2832, 3184
Bse8I GATNNNNATC 1 cut(s) 1250
BseAI TCCGGA 2 cut(s) 1487, 3058
BseBI CCWGG 3 cut(s) 1324, 2105, 2868
BseCI ATCGAT 2 cut(s) 1926, 3162
BseGI GGATG 5 cut(s) 1101, 2099, 2721, 2776, 3214
BseJI GATNNNNATC 1 cut(s) 1250
BseLI CCNNNNNNNGG 8 cut(s) 461, 1403, 1967, 2187, 2259, 2546, 3173, 3205
BseMI GCAATG 3 cut(s) 2127, 2832, 3184
BseMII CTCAG 7 cut(s) 565, 737, 940, 1070, 1154, 1378, 1886
BseNI ACTGG 2 cut(s) 2660, 2703
BseRI GAGGAG 2 cut(s) 226, 526
BseSI GKGCMC 2 cut(s) 1100, 2278
BseXI GCAGC 5 cut(s) 102, 414, 559, 2647, 2940
BseYI CCCAGC 2 cut(s) 955, 959
BsgI GTGCAG 3 cut(s) 109, 705, 1252
Bsh1236I CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
BshNI GGYRCC 3 cut(s) 1097, 2273, 2861
BshVI ATCGAT 2 cut(s) 1926, 3162
BsiHKAI GWGCWC 6 cut(s) 417, 702, 752, 1404, 2852, 3066
BsiHKCI CYCGRG 3 cut(s) 1580, 2277, 2348
BslFI GGGAC 5 cut(s) 137, 1891, 2522, 2709, 2861
BslI CCNNNNNNNGG 8 cut(s) 461, 1403, 1967, 2187, 2259, 2546, 3173, 3205
BsmAI GTCTC 4 cut(s) 1593, 1973, 2088, 2797
BsmBI CGTCTC 2 cut(s) 1593, 1973
BsmFI GGGAC 5 cut(s) 137, 1891, 2522, 2709, 2861
BsmI GAATGC 1 cut(s) 1737
BsoBI CYCGRG 3 cut(s) 1580, 2277, 2348
Bsp1286I GDGCHC 9 cut(s) 417, 702, 752, 966, 1100, 1404, 2278, 2852, 3066
Bsp13I TCCGGA 2 cut(s) 1487, 3058
Bsp19I CCATGG 3 cut(s) 1561, 3143, 3232
Bsp68I TCGCGA 3 cut(s) 905, 1137, 2484
BspCNI CTCAG 7 cut(s) 564, 738, 939, 1069, 1155, 1377, 1887
BspDI ATCGAT 2 cut(s) 1926, 3162
BspEI TCCGGA 2 cut(s) 1487, 3058
BspFNI CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
BspHI TCATGA 1 cut(s) 3066
BspMAI CTGCAG 2 cut(s) 404, 2497
BspMI ACCTGC 2 cut(s) 407, 1580
BspPI GGATC 6 cut(s) 323, 930, 1024, 1440, 3050, 3158
BspT107I GGYRCC 3 cut(s) 1097, 2273, 2861
BsrDI GCAATG 3 cut(s) 2127, 2832, 3184
BsrFI RCCGGY 2 cut(s) 1022, 1888
BsrI ACTGG 2 cut(s) 2660, 2703
BssAI RCCGGY 2 cut(s) 1022, 1888
BssNAI GTATAC 1 cut(s) 2410
BssNI GRCGYC 2 cut(s) 150, 3151
BssSI CACGAG 1 cut(s) 3005
BssT1I CCWWGG 5 cut(s) 1195, 1561, 2545, 3143, 3232
Bst1107I GTATAC 1 cut(s) 2410
Bst2BI CACGAG 1 cut(s) 3005
Bst2UI CCWGG 3 cut(s) 1324, 2105, 2868
Bst4CI ACNGT 7 cut(s) 231, 329, 587, 938, 1052, 1412, 1943
Bst6I CTCTTC 3 cut(s) 205, 456, 2086
BstACI GRCGYC 2 cut(s) 150, 3151
BstAPI GCANNNNNTGC 1 cut(s) 1557
BstC8I GCNNGC 7 cut(s) 172, 407, 545, 903, 2641, 2860, 3082
BstDSI CCRYGG 3 cut(s) 1561, 3143, 3232
BstF5I GGATG 5 cut(s) 1101, 2099, 2721, 2776, 3214
BstFNI CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 2 cut(s) 389, 530
BstMAI GTCTC 4 cut(s) 1593, 1973, 2088, 2797
BstNI CCWGG 3 cut(s) 1324, 2105, 2868
BstNSI RCATGY 1 cut(s) 1762
BstPAI GACNNNNGTC 1 cut(s) 522
BstSCI CCNGG 7 cut(s) 1322, 1395, 2103, 2276, 2277, 2866, 3198
BstSFI CTRYAG 4 cut(s) 400, 1993, 2493, 2973
BstSLI GKGCMC 2 cut(s) 1100, 2278
BstUI CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
BstV1I GCAGC 5 cut(s) 102, 414, 559, 2647, 2940
BstV2I GAAGAC 1 cut(s) 2971
BstX2I RGATCY 5 cut(s) 112, 315, 922, 1432, 2341
BstXI CCANNNNNNTGG 1 cut(s) 1166
BstYI RGATCY 5 cut(s) 112, 315, 922, 1432, 2341
BstZ17I GTATAC 1 cut(s) 2410
Bsu15I ATCGAT 2 cut(s) 1926, 3162
BsuI GTATCC 1 cut(s) 2543
BsuTUI ATCGAT 2 cut(s) 1926, 3162
BtgI CCRYGG 3 cut(s) 1561, 3143, 3232
BtgZI GCGATG 1 cut(s) 1480
BtsCI GGATG 5 cut(s) 1101, 2099, 2721, 2776, 3214
BtsI GCAGTG 4 cut(s) 1506, 2119, 2440, 2490
BtsIMutI CAGTG 5 cut(s) 743, 1506, 2119, 2440, 2490
BtuMI TCGCGA 3 cut(s) 905, 1137, 2484
BveI ACCTGC 2 cut(s) 407, 1580
Cac8I GCNNGC 7 cut(s) 172, 407, 545, 903, 2641, 2860, 3082
CaiI CAGNNNCTG 1 cut(s) 2249
CciI TCATGA 1 cut(s) 3066
CfoI GCGC 2 cut(s) 389, 530
Cfr10I RCCGGY 2 cut(s) 1022, 1888
Cfr9I CCCGGG 1 cut(s) 2277
ClaI ATCGAT 2 cut(s) 1926, 3162
CpoI CGGWCCG 1 cut(s) 587
CseI GACGC 2 cut(s) 3086, 3140
Csp6I GTAC 4 cut(s) 1000, 1192, 1762, 2590
CspCI CAANNNNNGTGG 2 cut(s) 1291, 1326
CspI CGGWCCG 1 cut(s) 587
CviQI GTAC 4 cut(s) 1000, 1192, 1762, 2590
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 3 cut(s) 205, 456, 2086
EarI CTCTTC 3 cut(s) 205, 456, 2086
EciI GGCGGA 3 cut(s) 166, 229, 772
Ecl136II GAGCTC 4 cut(s) 700, 750, 1402, 3064
Eco130I CCWWGG 5 cut(s) 1195, 1561, 2545, 3143, 3232
Eco24I GRGCYC 5 cut(s) 702, 752, 966, 1404, 3066
Eco47I GGWCC 4 cut(s) 481, 587, 875, 2685
Eco47III AGCGCT 1 cut(s) 388
Eco53kI GAGCTC 4 cut(s) 700, 750, 1402, 3064
Eco57I CTGAAG 1 cut(s) 989
Eco88I CYCGRG 3 cut(s) 1580, 2277, 2348
EcoICRI GAGCTC 4 cut(s) 700, 750, 1402, 3064
EcoO109I RGGNCCY 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 2486
EcoRII CCWGG 3 cut(s) 1322, 2103, 2866
EcoT14I CCWWGG 5 cut(s) 1195, 1561, 2545, 3143, 3232
EcoT38I GRGCYC 5 cut(s) 702, 752, 966, 1404, 3066
ErhI CCWWGG 5 cut(s) 1195, 1561, 2545, 3143, 3232
Esp3I CGTCTC 2 cut(s) 1593, 1973
FalI AAGNNNNNCTT 2 cut(s) 2559, 2591
FaqI GGGAC 5 cut(s) 137, 1891, 2522, 2709, 2861
FauI CCCGC 2 cut(s) 962, 1867
FbaI TGATCA 1 cut(s) 1143
FblI GTMKAC 1 cut(s) 2409
Fnu4HI GCNGC 8 cut(s) 91, 165, 168, 403, 548, 2661, 2954, 3149
FokI GGATG 5 cut(s) 1088, 2086, 2708, 2783, 3221
FriOI GRGCYC 5 cut(s) 702, 752, 966, 1404, 3066
Fsp4HI GCNGC 8 cut(s) 91, 165, 168, 403, 548, 2661, 2954, 3149
FspBI CTAG 4 cut(s) 978, 1349, 1647, 1860
GlaI GCGC 2 cut(s) 388, 529
GluI GCNGC 8 cut(s) 91, 165, 168, 403, 548, 2661, 2954, 3149
GsaI CCCAGC 2 cut(s) 959, 963
GsuI CTGGAG 1 cut(s) 431
HaeII RGCGCY 1 cut(s) 390
HgaI GACGC 2 cut(s) 3086, 3140
HhaI GCGC 2 cut(s) 389, 530
Hin1I GRCGYC 2 cut(s) 150, 3151
Hin6I GCGC 2 cut(s) 387, 528
HinP1I GCGC 2 cut(s) 387, 528
HindIII AAGCTT 4 cut(s) 1111, 1130, 1337, 2605
Hpy166II GTNNAC 4 cut(s) 396, 1020, 1654, 2410
Hpy188I TCNGA 7 cut(s) 148, 325, 591, 1008, 1598, 1896, 2797
Hpy8I GTNNAC 4 cut(s) 396, 1020, 1654, 2410
Hpy99I CGWCG 6 cut(s) 308, 329, 521, 587, 3243, 3246
HpyAV CCTTC 8 cut(s) 196, 352, 985, 1013, 1116, 1479, 2330, 3106
HpyCH4III ACNGT 7 cut(s) 231, 329, 587, 938, 1052, 1412, 1943
HpyCH4IV ACGT 7 cut(s) 150, 303, 519, 1523, 1676, 1982, 2760
HpySE526I ACGT 7 cut(s) 150, 303, 519, 1523, 1676, 1982, 2760
Hsp92I GRCGYC 2 cut(s) 150, 3151
HspAI GCGC 2 cut(s) 387, 528
Kpn2I TCCGGA 2 cut(s) 1487, 3058
Ksp22I TGATCA 1 cut(s) 1143
Lsp1109I GCAGC 5 cut(s) 102, 414, 559, 2647, 2940
LweI GCATC 5 cut(s) 730, 2157, 2301, 2652, 2730
MaeI CTAG 4 cut(s) 978, 1349, 1647, 1860
MaeII ACGT 7 cut(s) 150, 303, 519, 1523, 1676, 1982, 2760
MaeIII GTNAC 6 cut(s) 335, 627, 1367, 1406, 2127, 2234
MfeI CAATTG 2 cut(s) 2420, 2716
MflI RGATCY 5 cut(s) 112, 315, 922, 1432, 2341
MhlI GDGCHC 9 cut(s) 417, 702, 752, 966, 1100, 1404, 2278, 2852, 3066
MlyI GAGTC 4 cut(s) 5, 1973, 2799, 3207
MmeI TCCRAC 8 cut(s) 293, 348, 456, 744, 957, 1410, 1693, 3179
MroI TCCGGA 2 cut(s) 1487, 3058
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 6 cut(s) 671, 839, 1259, 1680, 1842, 2288
MslI CAYNNNNRTG 2 cut(s) 1499, 2841
MspA1I CMGCKG 4 cut(s) 959, 987, 1162, 2469
MspR9I CCNGG 7 cut(s) 1324, 1397, 2105, 2278, 2279, 2868, 3200
MunI CAATTG 2 cut(s) 2420, 2716
Mva1269I GAATGC 1 cut(s) 1737
MvaI CCWGG 3 cut(s) 1324, 2105, 2868
MvnI CGCG 5 cut(s) 528, 905, 1137, 2484, 2690
NciI CCSGG 4 cut(s) 1397, 2278, 2279, 3200
NcoI CCATGG 3 cut(s) 1561, 3143, 3232
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 3 cut(s) 335, 627, 1406
NruI TCGCGA 3 cut(s) 905, 1137, 2484
NspI RCATGY 1 cut(s) 1762
PaeR7I CTCGAG 2 cut(s) 1580, 2348
PagI TCATGA 1 cut(s) 3066
PaqCI CACCTGC 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PctI GAATGC 1 cut(s) 1737
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 9 cut(s) 20, 592, 677, 767, 1210, 1246, 1957, 2204, 2978
PflMI CCANNNNNTGG 2 cut(s) 2259, 3173
PkrI GCNGC 8 cut(s) 92, 166, 169, 404, 549, 2662, 2955, 3150
PleI GAGTC 4 cut(s) 5, 1972, 2798, 3207
PpsI GAGTC 4 cut(s) 5, 1972, 2798, 3207
PshAI GACNNNNGTC 1 cut(s) 522
Psp124BI GAGCTC 4 cut(s) 702, 752, 1404, 3066
Psp1406I AACGTT 1 cut(s) 1676
Psp6I CCWGG 3 cut(s) 1322, 2103, 2866
PspFI CCCAGC 2 cut(s) 955, 959
PspGI CCWGG 3 cut(s) 1322, 2103, 2866
PspXI VCTCGAGB 1 cut(s) 1580
PstI CTGCAG 2 cut(s) 404, 2497
PstNI CAGNNNCTG 1 cut(s) 2249
PsuI RGATCY 5 cut(s) 112, 315, 922, 1432, 2341
PvuII CAGCTG 3 cut(s) 959, 987, 1162
RruI TCGCGA 3 cut(s) 905, 1137, 2484
RsaI GTAC 4 cut(s) 1001, 1193, 1763, 2591
RsaNI GTAC 4 cut(s) 1000, 1192, 1762, 2590
RseI CAYNNNNRTG 2 cut(s) 1499, 2841
Rsr2I CGGWCCG 1 cut(s) 587
RsrII CGGWCCG 1 cut(s) 587
SacI GAGCTC 4 cut(s) 702, 752, 1404, 3066
SaqAI TTAA 6 cut(s) 671, 839, 1259, 1680, 1842, 2288
SatI GCNGC 8 cut(s) 91, 165, 168, 403, 548, 2661, 2954, 3149
SchI GAGTC 4 cut(s) 5, 1973, 2799, 3207
ScrFI CCNGG 7 cut(s) 1324, 1397, 2105, 2278, 2279, 2868, 3200
SduI GDGCHC 9 cut(s) 417, 702, 752, 966, 1100, 1404, 2278, 2852, 3066
SfaNI GCATC 5 cut(s) 730, 2157, 2301, 2652, 2730
SfcI CTRYAG 4 cut(s) 400, 1993, 2493, 2973
Sfr274I CTCGAG 2 cut(s) 1580, 2348
SinI GGWCC 4 cut(s) 481, 587, 875, 2685
SlaI CTCGAG 2 cut(s) 1580, 2348
SmaI CCCGGG 1 cut(s) 2279
SmiMI CAYNNNNRTG 2 cut(s) 1499, 2841
SmlI CTYRAG 7 cut(s) 1091, 1205, 1358, 1580, 2348, 2948, 3086
SmoI CTYRAG 7 cut(s) 1091, 1205, 1358, 1580, 2348, 2948, 3086
SspMI CTAG 4 cut(s) 978, 1349, 1647, 1860
SstI GAGCTC 4 cut(s) 702, 752, 1404, 3066
StyD4I CCNGG 7 cut(s) 1322, 1395, 2103, 2276, 2277, 2866, 3198
StyI CCWWGG 5 cut(s) 1195, 1561, 2545, 3143, 3232
TaaI ACNGT 7 cut(s) 231, 329, 587, 938, 1052, 1412, 1943
TaiI ACGT 7 cut(s) 153, 306, 522, 1526, 1679, 1985, 2763
TatI WGTACW 2 cut(s) 1761, 2589
TauI GCSGC 3 cut(s) 167, 170, 3151
TfiI GAWTC 9 cut(s) 20, 592, 677, 767, 1210, 1246, 1957, 2204, 2978
Tru1I TTAA 6 cut(s) 671, 839, 1259, 1680, 1842, 2288
Tru9I TTAA 6 cut(s) 671, 839, 1259, 1680, 1842, 2288
TscAI CASTG 5 cut(s) 750, 1506, 2119, 2440, 2497
TseFI GTSAC 3 cut(s) 335, 627, 1406
TseI GCWGC 5 cut(s) 90, 402, 547, 2660, 2953
Tsp45I GTSAC 3 cut(s) 335, 627, 1406
TspGWI ACGGA 5 cut(s) 299, 444, 1026, 1931, 3259
TspMI CCCGGG 1 cut(s) 2277
TspRI CASTG 5 cut(s) 750, 1506, 2119, 2440, 2497
Van91I CCANNNNNTGG 2 cut(s) 2259, 3173
VpaK11BI GGWCC 4 cut(s) 481, 587, 875, 2685
XapI RAATTY 6 cut(s) 866, 1504, 1573, 1780, 2486, 2776
XceI RCATGY 1 cut(s) 1762
XcmI CCANNNNNNNNNTGG 2 cut(s) 2426, 3201
XhoI CTCGAG 2 cut(s) 1580, 2348
XmaI CCCGGG 1 cut(s) 2277
XmiI GTMKAC 1 cut(s) 2409
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 4 cut(s) 978, 1349, 1647, 1860
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.