Rw2G021400
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012455
Physical Location & Seq
Reverse (-)
29701298 .. 29704842
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021400.1

Sequence Viewer

Length: 3513 bp
ATGAAACCCCACAAAACCATTTCACACCACTTCTTTCTCCTCCTCCTCTTCTTGCTCTCTGCCACTCCCTCTTCTTCCTCTTCAGCTTACAAAGACGCCCAGCAACTCCTGAGCTTCAAAAACTCTCTTCCGAAACCTACCCTTCTCTCCGATTGGCTCCCTGACCAAAACCCATGTCTGTTTTCCGGCGTTTTCTGCAAACAAACCCGGGTTTCCTCCATAGACCTGAGCTTCGTCACCTTGTCCACAAACCTCACAATTATCTCCACTTTCTTGATGACAATTGATTCTCTACAATCACTCACTCTGAAATCTACCGGCCTCACCGGCCCCATTTCTTTTCCGGCTAAATCCAAGTGCAGCCCTCTCCTCACTTACATAGATCTAGCTGAGAACAGCTTGTCCGGCCCAATTTCCGGTATTCCCAGCTTGGCTTCCTGCTCGAGCTTGAACTCTCTTAACCTCTCCGCCAACCTCCTCGAGTTCAATGCCAAGGACTCGGCCACGTTCGTACTCTCCCTCCAGGTCCTCGACCTCTCCTTCAACAAGATTGCCGGCCCCGCCGTGCCGTGGATCGTATCCGACGGCTGCAGCGAGCTTGTAAAAGTTCTCAAGGGGAACAAGATCACCGGCGACATGAGTGTCTCTGGGTGCAAGAAGCTCGAGACTCTCGACCTCTCCTCCAACAATTTCACGGTGGAGATTCCGTCTTTCGGCGACTGTATGGTTCTGGACCATCTCGACATCTCCGGCAACAAATTCTCCGGCGACGTCGCGCACGCTCTCTCTTCCTGCAAGCAACTGACGTTTCTGAACCTCTCGAGAAACCATTTCTCCGGTCCCATTCCGGCCATTCCCACCCAGAATCTTGAGTTTCTTTCCCTCTCCGGCAATGGGTTCCAAGATACAATTCCACCCAGCTTGCTCAGTTCGTGTGAGTCGCTAGTGGAGCTGGATCTTTCCATAAATAACCTCTCGGGCATCGTTCCCGTTGCATTGAGCTCTTGCGCTTCCTTGGAGTCTCTCGACATATCCAACAACTTGTTCACCGGTGAGTTGCCCATCGAAACTCTGTTGAAGCTCAGCAGCCTGAAGAGCGTGTCTCTTTCTCTCAACGGTTTCTCTGGTACTCTGCCTCGCTCTCTCTCCAACCTGTCATATATAGACAGCTTGGATCTGAGCTCGAACAATTTCACTGGCTCCATTCCGAGTTGGCTTTGCGAAGATCCCAGGAACAGCTGGAAGGAGCTGTACCTCCAGAACAATAAGTTCGGTGGTACCATTCCTCCAGCTCTCAGCAACTGCACTCAGCTCGTTGCTCTTGATTTGAGCTTCAACTACCTCACTGGTACCATCCCCCTAGCTTGGGGTACTTTGTCCAAGCTTCGCGACTTGATCCTTTGGTTGAATCAGCTTAGCGGGGAAATTCCCCAGGAGCTCACGAATCTTGGGTTGCTGGAGAATCTTATCCTGGACTTCAATGAGCTGACGGGGACGATTCCTGTGGGGCTGAGTAACTGCACCAATTTGAGCTGGATTTCGTTGGCGAATAACAAGTTGAGTGGTGAGATTCCGGCGTGGATTGGCAAGCTTCCGAAGCTGGCAATACTGAAGCTGAGTAACAACTCATTCTATGGGAACATTCCCCCGGAGCTGGGGGACTGTAAGAGCTTGATATGGTTGGATCTCAATACTAACTTGCTGAATGGTACAATCCCTCCTGGGCTTTTCAAACAGTCTGGCAACATTGCGGTGAATTTTGTTGCTTCCAAGACGTATGTGTATATCAAGAATGATGGAAGCAAGGAGTGCCATGGAGCAGGGAATTTGCTCGAGTTTGCGGGGATAAGACAAGAGCAATTGACCAGGATTTCGACCAGGAACCCCTGCAACTTCACTAGAGTGTATAGAGGTATTCTCCAACCAACATTTAATCACAATGGGACCATGATTTTTCTTGATATGTCACACAATAGGTTATCGGGTAGCATTCCGAAAGAGATTGGGAGTATGTACTATCTCTATATTCTGAATCTGGGCCATAATAATATATCTGGAGCAATCCCGCTAGAGCTTGGGAAATTGAAGGACCTTAACATTCTTGATCTCTCTAGCAACAGCCTTGATGGAAGTATTCCTCAGTCTTTGGTCAGCCTTTCCATGCTGATGGAGATCGATTTGTCAAACAATCATCTATCTGGAATGATTCCAGATTCAGGCCAGTTTGAGACATTCCCTGCTTACAGATTTATGAACAATTCAGGCCTTTGTGGCTACCCTCTAAATTCCTGTGGAGCTGCTTCTGGGGCAAATGGAAATGGGCACCAGAAATCTCATCGACCAGCGTCCCTGGCAGGCAGTGTGGCAATGGGGCTACTGTTTTCCCTTTTCTGCATCTTTGGTTTGCTTATTAAGGATTCAGGTCTGGACGTTTATGTGGACAGTCGTTCCCACTCCGGTACTGCCTGGAAGCTAACGGGTGCCCGAGAAGCATTAAGCATTAACCTCTCCACATTTGAGAAGCCACTTCAAAAGCTCACTTTTGCAGATCTTCTTGAGGCTACCAATGGTTTCCACAATGACAGTCTTATAGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAGGATGGCAGCATTGTTGCCATCAAGAAGTTGATACATATCAGCGGACAAGGTGATCGTGAATTCACTGCTGAAATGGAAACTATCGGGAAGATCAAGCACCGAAACCTTGTTCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTAGTTTACGAGTACATGAAGTATGGAAGCTTAGATGATGTTTTACATGACCAGAAGAAAGGTATCAAGCTGAGTTGGTCTGCAAGGAGGAAGATTGCGATTGGATCTGCGAGAGGACTGGCCTTTCTTCACCACAATTGCATTCCTCACATCATTCATAGGGACATGAAATCGAGCAACGTCCTGCTCGATGAGAATCTAGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTGTGAGTGCAATGGATACTCATTTGAGTGTGAGCACCCTGGCAGGCACACCTGGTTATGTTCCTCCAGAATACTACCAGAGTTTCAGATGTTCTACAAAAGGCGATGTTTATAGTTATGGTGTAGTATTGCTGGAGCTGCTAACAGGAAGACGTCCTACAGATTCAGCTGATTTTGGTGACAATAATCTTGTTGGTTGGGTGAAGCAACATGCTAAATTAAAAATAAGTGATGTTTTTGATCCAGAGCTCATGAAAGAGGACCCAACCCTTGAGATTGAGCTGTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGATGATAGGCCATGGCGCCGCCCTACGATGATTCAAGTCATGGCGCAGTTCAAGGAAATCCAAGCAGGACCCGGGATGGACTCCCAATCAACCATAGCCACCGACGATGGAGGTTTTGGTGCAGTTGAAATGGTTGAGATGAGCATAAAAGAAGACCCTGAACCGGGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1170

Amino Acids

127.23

Weight (kDa)

5.85

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 66 3e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 220 - 278 2.8e-07 Leucine rich repeat
LRR_14 PF23598 234 - 469 4.6e-07 Leucine-rich repeat region
LRR_8 PF13855 289 - 348 2e-08 Leucine rich repeat
LRR_8 PF13855 486 - 543 4.8e-07 Leucine rich repeat
Island PF20141 581 - 645 8.6e-32 Brassinosteroid receptor island domain
LRR_14 PF23598 654 - 729 8.8e-06 Leucine-rich repeat region
LRR_8 PF13855 674 - 732 2.3e-06 Leucine rich repeat
Pkinase PF00069 858 - 1127 1.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 861 - 1127 8.7e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AatII GACGTC 2 cut(s) 774, 3175
Acc65I GGTACC 2 cut(s) 1277, 1349
AccB1I GGYRCC 5 cut(s) 1277, 1349, 2322, 2480, 3351
AccI GTMKAC 1 cut(s) 2616
AccII CGCG 2 cut(s) 776, 1389
AciI CCGC 8 cut(s) 468, 561, 1419, 1751, 1841, 2066, 2676, 3355
AclWI GGATC 8 cut(s) 581, 963, 1182, 1220, 1390, 1692, 2896, 3254
AcoI YGGCCR 2 cut(s) 501, 849
AcsI RAATTY 6 cut(s) 758, 1425, 1756, 1825, 2284, 2693
AcuI CTGAAG 3 cut(s) 66, 1112, 1631
AcyI GRCGYC 4 cut(s) 96, 771, 3172, 3352
AgeI ACCGGT 1 cut(s) 1049
AjuI GAANNNNNNNTTGG 4 cut(s) 894, 926, 1028, 1060
AleI CACNNNNGTG 1 cut(s) 1901
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
Alw21I GWGCWC 5 cut(s) 1004, 1184, 1440, 3056, 3270
Alw26I GTCTC 6 cut(s) 649, 659, 1026, 1107, 2222, 3001
AlwI GGATC 8 cut(s) 581, 963, 1182, 1220, 1390, 1692, 2896, 3254
AlwNI CAGNNNCTG 1 cut(s) 1302
Ama87I CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
ApeKI GCWGC 7 cut(s) 360, 588, 591, 1086, 2297, 2640, 3157
ApoI RAATTY 6 cut(s) 758, 1425, 1756, 1825, 2284, 2693
ArsI GACNNNNNNTTYG 6 cut(s) 160, 192, 215, 247, 1855, 1887
AsiGI ACCGGT 1 cut(s) 1049
Asp700I GAANNNNTTC 1 cut(s) 1190
Asp718I GGTACC 2 cut(s) 1277, 1349
AspLEI GCGC 4 cut(s) 778, 1010, 3354, 3382
AsuC2I CCSGG 6 cut(s) 208, 209, 1649, 3408, 3409, 3501
AvaI CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
AvaII GGWCC 7 cut(s) 526, 733, 839, 1944, 2089, 3280, 3404
BaeGI GKGCMC 2 cut(s) 2325, 2485
BanI GGYRCC 5 cut(s) 1277, 1349, 2322, 2480, 3351
BanII GRGCYC 4 cut(s) 1004, 1184, 1440, 3270
BarI GAAGNNNNNNTAC 4 cut(s) 1235, 1267, 3160, 3192
BbsI GAAGAC 2 cut(s) 3175, 3495
Bbv12I GWGCWC 5 cut(s) 1004, 1184, 1440, 3056, 3270
BbvI GCAGC 7 cut(s) 372, 575, 603, 1098, 2284, 2652, 3144
BceAI ACGGC 4 cut(s) 548, 553, 601, 2795
BcgI CGANNNNNNTGC 6 cut(s) 470, 504, 643, 677, 1294, 1328
BciVI GTATCC 3 cut(s) 589, 2750, 3028
BcnI CCSGG 6 cut(s) 208, 209, 1649, 3408, 3409, 3501
BcoDI GTCTC 6 cut(s) 649, 659, 1026, 1107, 2222, 3001
BfaI CTAG 7 cut(s) 386, 944, 1361, 1899, 2069, 2112, 2984
BfmI CTRYAG 2 cut(s) 589, 3177
BfoI RGCGCY 1 cut(s) 3355
BfuI GTATCC 3 cut(s) 589, 2750, 3028
BglI GCCNNNNNGGC 2 cut(s) 327, 2271
BglII AGATCT 2 cut(s) 382, 2548
BisI GCNGC 8 cut(s) 361, 589, 592, 1087, 2298, 2641, 3158, 3355
BlpI GCTNAGC 2 cut(s) 1082, 1415
BlsI GCNGC 8 cut(s) 362, 590, 593, 1088, 2299, 2642, 3159, 3356
Bme18I GGWCC 7 cut(s) 526, 733, 839, 1944, 2089, 3280, 3404
BmeT110I CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
BmsI GCATC 2 cut(s) 990, 2403
BoxI GACNNNNGTC 1 cut(s) 2344
BpiI GAAGAC 2 cut(s) 3175, 3495
BplI GAGNNNNNCTC 4 cut(s) 1087, 1119, 1902, 1934
BpmI CTGGAG 7 cut(s) 506, 1241, 1272, 1478, 2076, 3069, 3173
Bpu10I CCTNAGC 2 cut(s) 110, 227
Bpu1102I GCTNAGC 2 cut(s) 1082, 1415
BpuEI CTTGAG 4 cut(s) 596, 890, 2576, 3311
BpuMI CCSGG 6 cut(s) 208, 209, 1649, 3408, 3409, 3501
Bsa29I ATCGAT 1 cut(s) 2175
BsaBI GATNNNNATC 3 cut(s) 2171, 2669, 2979
BsaHI GRCGYC 4 cut(s) 96, 771, 3172, 3352
BsaWI WCCGGW 4 cut(s) 416, 836, 1049, 2456
Bse118I RCCGGY 5 cut(s) 317, 326, 554, 629, 1049
Bse1I ACTGG 4 cut(s) 1201, 1351, 2221, 2907
Bse3DI GCAATG 4 cut(s) 898, 1746, 2373, 3036
Bse8I GATNNNNATC 3 cut(s) 2171, 2669, 2979
BseCI ATCGAT 1 cut(s) 2175
BseGI GGATG 4 cut(s) 1353, 2641, 3343, 3417
BseJI GATNNNNATC 3 cut(s) 2171, 2669, 2979
BseMI GCAATG 4 cut(s) 898, 1746, 2373, 3036
BseNI ACTGG 4 cut(s) 1201, 1351, 2221, 2907
BseRI GAGGAG 6 cut(s) 29, 32, 35, 359, 467, 670
BseSI GKGCMC 2 cut(s) 2325, 2485
BseXI GCAGC 7 cut(s) 372, 575, 603, 1098, 2284, 2652, 3144
BseYI CCCAGC 4 cut(s) 99, 425, 917, 1654
BsgI GTGCAG 4 cut(s) 379, 1288, 1504, 3477
Bsh1236I CGCG 2 cut(s) 776, 1389
BshNI GGYRCC 5 cut(s) 1277, 1349, 2322, 2480, 3351
BshTI ACCGGT 1 cut(s) 1049
BshVI ATCGAT 1 cut(s) 2175
BsiHKAI GWGCWC 5 cut(s) 1004, 1184, 1440, 3056, 3270
BsiHKCI CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
BslFI GGGAC 6 cut(s) 825, 1507, 1673, 1957, 2332, 2960
BsmAI GTCTC 6 cut(s) 649, 659, 1026, 1107, 2222, 3001
BsmFI GGGAC 6 cut(s) 825, 1507, 1673, 1957, 2332, 2960
BsmI GAATGC 2 cut(s) 1989, 2925
BsoBI CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
Bsp1286I GDGCHC 7 cut(s) 1004, 1184, 1440, 2325, 2485, 3056, 3270
Bsp1720I GCTNAGC 2 cut(s) 1082, 1415
Bsp19I CCATGG 2 cut(s) 1813, 3347
Bsp68I TCGCGA 1 cut(s) 1389
BspACI CCGC 8 cut(s) 468, 561, 1419, 1751, 1841, 2066, 2676, 3355
BspDI ATCGAT 1 cut(s) 2175
BspFNI CGCG 2 cut(s) 776, 1389
BspHI TCATGA 1 cut(s) 3270
BspMAI CTGCAG 1 cut(s) 593
BspPI GGATC 8 cut(s) 581, 963, 1182, 1220, 1390, 1692, 2896, 3254
BspQI GCTCTTC 1 cut(s) 1088
BspT107I GGYRCC 5 cut(s) 1277, 1349, 2322, 2480, 3351
BsrDI GCAATG 4 cut(s) 898, 1746, 2373, 3036
BsrFI RCCGGY 5 cut(s) 317, 326, 554, 629, 1049
BsrI ACTGG 4 cut(s) 1201, 1351, 2221, 2907
BssAI RCCGGY 5 cut(s) 317, 326, 554, 629, 1049
BssNAI GTATAC 1 cut(s) 2617
BssNI GRCGYC 4 cut(s) 96, 771, 3172, 3352
BssT1I CCWWGG 5 cut(s) 492, 1014, 1813, 2752, 3347
Bst1107I GTATAC 1 cut(s) 2617
Bst4CI ACNGT 8 cut(s) 697, 722, 1118, 1664, 1737, 2379, 2444, 2585
Bst6I CTCTTC 6 cut(s) 53, 76, 85, 132, 793, 1088
BstACI GRCGYC 4 cut(s) 96, 771, 3172, 3352
BstAPI GCANNNNNTGC 1 cut(s) 1809
BstDSI CCRYGG 3 cut(s) 569, 1813, 3347
BstF5I GGATG 4 cut(s) 1353, 2641, 3343, 3417
BstFNI CGCG 2 cut(s) 776, 1389
BstH2I RGCGCY 1 cut(s) 3355
BstHHI GCGC 4 cut(s) 778, 1010, 3354, 3382
BstMAI GTCTC 6 cut(s) 649, 659, 1026, 1107, 2222, 3001
BstNSI RCATGY 1 cut(s) 3233
BstPAI GACNNNNGTC 1 cut(s) 2344
BstSFI CTRYAG 2 cut(s) 589, 3177
BstSLI GKGCMC 2 cut(s) 2325, 2485
BstUI CGCG 2 cut(s) 776, 1389
BstV1I GCAGC 7 cut(s) 372, 575, 603, 1098, 2284, 2652, 3144
BstV2I GAAGAC 2 cut(s) 3175, 3495
BstX2I RGATCY 7 cut(s) 382, 955, 1174, 1225, 1684, 2548, 2888
BstXI CCANNNNNNTGG 1 cut(s) 2167
BstYI RGATCY 7 cut(s) 382, 955, 1174, 1225, 1684, 2548, 2888
BstZ17I GTATAC 1 cut(s) 2617
Bsu15I ATCGAT 1 cut(s) 2175
BsuI GTATCC 3 cut(s) 589, 2750, 3028
BsuTUI ATCGAT 1 cut(s) 2175
BtgI CCRYGG 3 cut(s) 569, 1813, 3347
BtgZI GCGATG 1 cut(s) 3138
BtsCI GGATG 4 cut(s) 1353, 2641, 3343, 3417
BtsI GCAGTG 2 cut(s) 2365, 2697
BtsIMutI CAGTG 4 cut(s) 1194, 1344, 2365, 2697
BtuMI TCGCGA 1 cut(s) 1389
CaiI CAGNNNCTG 1 cut(s) 1302
CciI TCATGA 1 cut(s) 3270
CfoI GCGC 4 cut(s) 778, 1010, 3354, 3382
Cfr10I RCCGGY 5 cut(s) 317, 326, 554, 629, 1049
Cfr9I CCCGGG 2 cut(s) 207, 3407
ClaI ATCGAT 1 cut(s) 2175
CseI GACGC 2 cut(s) 104, 2334
CsiI ACCWGGT 1 cut(s) 3070
CspAI ACCGGT 1 cut(s) 1049
CspCI CAANNNNNGTGG 4 cut(s) 903, 938, 1543, 1578
DinI GGCGCC 1 cut(s) 3353
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 2 cut(s) 501, 849
Eam1104I CTCTTC 6 cut(s) 53, 76, 85, 132, 793, 1088
EarI CTCTTC 6 cut(s) 53, 76, 85, 132, 793, 1088
EciI GGCGGA 1 cut(s) 457
Ecl136II GAGCTC 4 cut(s) 1002, 1182, 1438, 3268
Eco130I CCWWGG 5 cut(s) 492, 1014, 1813, 2752, 3347
Eco147I AGGCCT 1 cut(s) 2265
Eco24I GRGCYC 4 cut(s) 1004, 1184, 1440, 3270
Eco47I GGWCC 7 cut(s) 526, 733, 839, 1944, 2089, 3280, 3404
Eco53kI GAGCTC 4 cut(s) 1002, 1182, 1438, 3268
Eco57I CTGAAG 3 cut(s) 66, 1112, 1631
Eco88I CYCGRG 9 cut(s) 207, 442, 479, 662, 820, 976, 1832, 2484, 3407
EcoICRI GAGCTC 4 cut(s) 1002, 1182, 1438, 3268
EcoO109I RGGNCCY 4 cut(s) 526, 2089, 3280, 3404
EcoRI GAATTC 1 cut(s) 2693
EcoT14I CCWWGG 5 cut(s) 492, 1014, 1813, 2752, 3347
EcoT38I GRGCYC 4 cut(s) 1004, 1184, 1440, 3270
EgeI GGCGCC 1 cut(s) 3353
EheI GGCGCC 1 cut(s) 3353
ErhI CCWWGG 5 cut(s) 492, 1014, 1813, 2752, 3347
FalI AAGNNNNNCTT 2 cut(s) 2766, 2798
FaqI GGGAC 6 cut(s) 825, 1507, 1673, 1957, 2332, 2960
FauI CCCGC 4 cut(s) 568, 1412, 1834, 2073
FblI GTMKAC 1 cut(s) 2616
Fnu4HI GCNGC 8 cut(s) 361, 589, 592, 1087, 2298, 2641, 3158, 3355
FokI GGATG 4 cut(s) 1340, 2648, 3350, 3424
FriOI GRGCYC 4 cut(s) 1004, 1184, 1440, 3270
Fsp4HI GCNGC 8 cut(s) 361, 589, 592, 1087, 2298, 2641, 3158, 3355
FspBI CTAG 7 cut(s) 386, 944, 1361, 1899, 2069, 2112, 2984
GlaI GCGC 4 cut(s) 777, 1009, 3353, 3381
GluI GCNGC 8 cut(s) 361, 589, 592, 1087, 2298, 2641, 3158, 3355
GsaI CCCAGC 4 cut(s) 103, 429, 921, 1658
GsuI CTGGAG 7 cut(s) 506, 1241, 1272, 1478, 2076, 3069, 3173
HaeII RGCGCY 1 cut(s) 3355
HgaI GACGC 2 cut(s) 104, 2334
HhaI GCGC 4 cut(s) 778, 1010, 3354, 3382
Hin1I GRCGYC 4 cut(s) 96, 771, 3172, 3352
Hin6I GCGC 4 cut(s) 776, 1008, 3352, 3380
HinP1I GCGC 4 cut(s) 776, 1008, 3352, 3380
HindIII AAGCTT 3 cut(s) 1382, 1589, 2812
Hpy166II GTNNAC 5 cut(s) 246, 1047, 2440, 2617, 2791
Hpy8I GTNNAC 5 cut(s) 246, 1047, 2440, 2617, 2791
Hpy99I CGWCG 4 cut(s) 587, 773, 776, 3443
HpyAV CCTTC 6 cut(s) 152, 550, 1237, 2080, 2626, 3310
HpyCH4III ACNGT 8 cut(s) 697, 722, 1118, 1664, 1737, 2379, 2444, 2585
HpyCH4IV ACGT 7 cut(s) 506, 771, 806, 1775, 2430, 2964, 3172
HpySE526I ACGT 7 cut(s) 506, 771, 806, 1775, 2430, 2964, 3172
Hsp92I GRCGYC 4 cut(s) 96, 771, 3172, 3352
HspAI GCGC 4 cut(s) 776, 1008, 3352, 3380
KasI GGCGCC 1 cut(s) 3351
KpnI GGTACC 2 cut(s) 1281, 1353
KroI GCCGGC 1 cut(s) 554
KroNI GCCGGC 1 cut(s) 556
LguI GCTCTTC 1 cut(s) 1088
Lsp1109I GCAGC 7 cut(s) 372, 575, 603, 1098, 2284, 2652, 3144
LweI GCATC 2 cut(s) 990, 2403
MabI ACCWGGT 1 cut(s) 3070
MaeI CTAG 7 cut(s) 386, 944, 1361, 1899, 2069, 2112, 2984
MaeII ACGT 7 cut(s) 506, 771, 806, 1775, 2430, 2964, 3172
MaeIII GTNAC 6 cut(s) 235, 1514, 1619, 1965, 3197, 3303
MfeI CAATTG 4 cut(s) 282, 1859, 2627, 2920
MflI RGATCY 7 cut(s) 382, 955, 1174, 1225, 1684, 2548, 2888
MhlI GDGCHC 7 cut(s) 1004, 1184, 1440, 2325, 2485, 3056, 3270
Mly113I GGCGCC 1 cut(s) 3352
MlyI GAGTC 6 cut(s) 491, 661, 947, 1028, 3003, 3410
MmeI TCCRAC 6 cut(s) 606, 708, 1059, 1173, 1662, 1945
MroNI GCCGGC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 1190
MseI TTAA 7 cut(s) 459, 1932, 2094, 2412, 2495, 2502, 3239
MslI CAYNNNNRTG 4 cut(s) 1751, 1901, 2165, 3045
MspA1I CMGCKG 3 cut(s) 1239, 2676, 3188
MunI CAATTG 4 cut(s) 282, 1859, 2627, 2920
Mva1269I GAATGC 2 cut(s) 1989, 2925
MvnI CGCG 2 cut(s) 776, 1389
NaeI GCCGGC 1 cut(s) 556
NarI GGCGCC 1 cut(s) 3352
NciI CCSGG 6 cut(s) 208, 209, 1649, 3408, 3409, 3501
NcoI CCATGG 2 cut(s) 1813, 3347
NgoMIV GCCGGC 1 cut(s) 554
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 3 cut(s) 235, 1965, 3197
NruI TCGCGA 1 cut(s) 1389
NspI RCATGY 1 cut(s) 3233
OliI CACNNNNGTG 1 cut(s) 1901
PaeR7I CTCGAG 5 cut(s) 442, 479, 662, 820, 1832
PagI TCATGA 1 cut(s) 3270
PceI AGGCCT 1 cut(s) 2265
PciSI GCTCTTC 1 cut(s) 1088
PcsI WCGNNNNNNNCGW 3 cut(s) 591, 669, 2970
PctI GAATGC 2 cut(s) 1989, 2925
PdiI GCCGGC 1 cut(s) 556
PdmI GAANNNNTTC 1 cut(s) 1190
PfoI TCCNGGA 1 cut(s) 1470
PinAI ACCGGT 1 cut(s) 1049
PkrI GCNGC 8 cut(s) 362, 590, 593, 1088, 2299, 2642, 3159, 3356
PleI GAGTC 6 cut(s) 491, 661, 946, 1027, 3002, 3410
PluTI GGCGCC 1 cut(s) 3355
PpsI GAGTC 6 cut(s) 491, 661, 946, 1027, 3002, 3410
PpuMI RGGWCCY 4 cut(s) 526, 2089, 3280, 3404
PshAI GACNNNNGTC 1 cut(s) 2344
Psp124BI GAGCTC 4 cut(s) 1004, 1184, 1440, 3270
Psp5II RGGWCCY 4 cut(s) 526, 2089, 3280, 3404
PspFI CCCAGC 4 cut(s) 99, 425, 917, 1654
PspPPI RGGWCCY 4 cut(s) 526, 2089, 3280, 3404
PspXI VCTCGAGB 3 cut(s) 442, 479, 1832
PstI CTGCAG 1 cut(s) 593
PstNI CAGNNNCTG 1 cut(s) 1302
PsuI RGATCY 7 cut(s) 382, 955, 1174, 1225, 1684, 2548, 2888
PvuII CAGCTG 2 cut(s) 1239, 3188
RruI TCGCGA 1 cut(s) 1389
RseI CAYNNNNRTG 4 cut(s) 1751, 1901, 2165, 3045
SacI GAGCTC 4 cut(s) 1004, 1184, 1440, 3270
SapI GCTCTTC 1 cut(s) 1088
SaqAI TTAA 7 cut(s) 459, 1932, 2094, 2412, 2495, 2502, 3239
SatI GCNGC 8 cut(s) 361, 589, 592, 1087, 2298, 2641, 3158, 3355
SchI GAGTC 6 cut(s) 491, 661, 947, 1028, 3003, 3410
SduI GDGCHC 7 cut(s) 1004, 1184, 1440, 2325, 2485, 3056, 3270
SexAI ACCWGGT 1 cut(s) 3070
SfaNI GCATC 2 cut(s) 990, 2403
SfcI CTRYAG 2 cut(s) 589, 3177
SfiI GGCCNNNNNGGCC 1 cut(s) 327
SfoI GGCGCC 1 cut(s) 3353
Sfr274I CTCGAG 5 cut(s) 442, 479, 662, 820, 1832
SgrAI CRCCGGYG 2 cut(s) 629, 1049
SinI GGWCC 7 cut(s) 526, 733, 839, 1944, 2089, 3280, 3404
SlaI CTCGAG 5 cut(s) 442, 479, 662, 820, 1832
SmaI CCCGGG 2 cut(s) 209, 3409
SmiMI CAYNNNNRTG 4 cut(s) 1751, 1901, 2165, 3045
SmlI CTYRAG 9 cut(s) 442, 479, 611, 662, 820, 869, 1832, 2555, 3290
SmoI CTYRAG 9 cut(s) 442, 479, 611, 662, 820, 869, 1832, 2555, 3290
SseBI AGGCCT 1 cut(s) 2265
SsiI CCGC 8 cut(s) 468, 561, 1419, 1751, 1841, 2066, 2676, 3355
SspDI GGCGCC 1 cut(s) 3351
SspMI CTAG 7 cut(s) 386, 944, 1361, 1899, 2069, 2112, 2984
SstI GAGCTC 4 cut(s) 1004, 1184, 1440, 3270
StuI AGGCCT 1 cut(s) 2265
StyI CCWWGG 5 cut(s) 492, 1014, 1813, 2752, 3347
TaaI ACNGT 8 cut(s) 697, 722, 1118, 1664, 1737, 2379, 2444, 2585
TaiI ACGT 7 cut(s) 509, 774, 809, 1778, 2433, 2967, 3175
TatI WGTACW 2 cut(s) 2013, 2796
TauI GCSGC 1 cut(s) 3357
Tru1I TTAA 7 cut(s) 459, 1932, 2094, 2412, 2495, 2502, 3239
Tru9I TTAA 7 cut(s) 459, 1932, 2094, 2412, 2495, 2502, 3239
TscAI CASTG 4 cut(s) 1201, 1351, 2365, 2704
TseFI GTSAC 3 cut(s) 235, 1965, 3197
TseI GCWGC 7 cut(s) 360, 588, 591, 1086, 2297, 2640, 3157
Tsp45I GTSAC 3 cut(s) 235, 1965, 3197
TspDTI ATGAA 6 cut(s) 17, 2267, 2816, 2930, 2966, 3287
TspGWI ACGGA 1 cut(s) 696
TspMI CCCGGG 2 cut(s) 207, 3407
TspRI CASTG 4 cut(s) 1201, 1351, 2365, 2704
VpaK11BI GGWCC 7 cut(s) 526, 733, 839, 1944, 2089, 3280, 3404
XapI RAATTY 6 cut(s) 758, 1425, 1756, 1825, 2284, 2693
XbaI TCTAGA 1 cut(s) 2983
XceI RCATGY 1 cut(s) 3233
XcmI CCANNNNNNNNNTGG 1 cut(s) 2633
XhoI CTCGAG 5 cut(s) 442, 479, 662, 820, 1832
XmaI CCCGGG 2 cut(s) 207, 3407
XmiI GTMKAC 1 cut(s) 2616
XmnI GAANNNNTTC 1 cut(s) 1190
XspI CTAG 7 cut(s) 386, 944, 1361, 1899, 2069, 2112, 2984
ZraI GACGTC 2 cut(s) 772, 3173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.