MD15G1336800.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012455
Physical Location & Seq
Forward (+)
37780741 .. 37784753
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1336800.v1.1.491

Sequence Viewer

Length: 3540 bp
ATGATATCCCACAAGTCCATTTCTCACTTCCCCTTCCCCTTCTGCCTCCTCCTCCTCCTCCTCTTCGTCCTCCCTCTGCCACCCACCTCTGCAACTCCGCCCACCTCCTCCTTTTACAGAGACACCCAGCAACTCCTCAGCTTCAAATCCTCCCTTCCAACCCCAACCCTCCTCTCCAACTGGCTCCCCAACCAAAACCCATGTTCCTTTTCTGGTATATCCTGCAAAGCAACCAGGGTTTCGTCCATAGACCTCTCCTCCTTCTCCCTCGCCACAAATCTGACTGTTGTTTCCACGTTCCTCATGACCCTCGACTCCCTTGAATCTCTCTCTCTCAATTCAGCCTCTCTCTCCGGCTCTATCTCCCTCCACTTCCCTCCCAGAACCAAGTGCAGCCCTCACCTCACCTCCTTAGATCTGGCTCACAACTCCCTTTCCGGCCCTCTTTCAGACGTCCCCGACTTCGCCGCCGCTTGCTCCGCCTTGACCTTCCTCAACCTCTCCTCAAACTCTCTGGTTTTACCCACCAAACCATCCTCCTCCGCCTTCCCCCTCCGCACCCTCCAAGTTCTCGATCTTTCTTACAACAAGATTACAGGCCCCAACGTCGTCCGTTGGATCTTATCCGACGGTTGCGGTGACTTGCAGAGGTTGGTTTTGAAAGGGAACAAAATCTCCGGCGAGATGAGCGTTGTGTCCACCTGCAGCAAGCTGGAGCACTTGGACTTGTCCTCCAACAACTTCTCCATTTCTCTTCCGTCTTTCGGGGATTGCTCGGCTTTGGACCACCTTGACATATCTGGCAACAAGTTTTCTGGCGACATTGGTCGAGCTATCTCCTCCTGCAAGCAGCTCAGTTTCTTGAACCTCTCCATGAACCACTTCGACGGTCCGATTCCGGCCATGCCCACCAACAGCTTGAAGTTCCTGTCCCTTGGAGGTAATAGGTTTCAAGGTATAATTCCTGTGAGCTTAATGGATTCGTGTGCAGAGCTTGTGGAGCTCGATCTGTCGGCTAATAGCCTTTCGGGTTCGGTTCCTGATGCACTGAGCTCTTGTTCTTCGTTGGAATCGCTGGACATATCCACCAATAACTTTTCTGGTGAGTTGCCCGTTGAGATTTTGATGAAGCTCACCAACTTAAAGGCTGTGTCGCTTTCTTTCAACAACTTCTTTGGTCCTCTGCCCAATTCTCTGTCTAAGCTCGCGACATTGGAGAGCTTGGATCTCAGCTCCAACAATTTGTCTGGGTCAATCCCAGCTGGGCTCTGCGGGGATCCTAGCAACAGCTGGAAAGAGCTGTACCTTCAGAACAATCTGTTTTCCGGCACGATCCCTCCGTCTTTGAGCAACTGTTCTCAGCTTGTTTCTCTTGATTTGAGCTTCAATTACCTAAAGGGCACCATCCCTTCGAGCTTGGGGTCGTTGTCGAAGCTTCGCGATTTGAACATTTGGTTGAACCAGCTGAGTGGGGAAATCCCACAAGAGCTGATGTACCTTGGGTCGCTTCAGAATCTCATTCTGGACTTCAACGACCTTACGGGGTCGATTCCTATCGGTTTAAGCAACTGCACCAATTTGAATTGGATTTCATTGGCTAACAACAAGTTGAGTGGTGAGGTTCCCGGGTGGATTGGGAAGCTTCCAAAGCTAGCAATACTCAAGCTCAGTAACAACTCATTCTCTGGTGATATTCCCCCGGAGCTCGGTGACTGTAAGAGCTTGATATGGTTGGATCTCAATTCCAACTTGTTGAATGGCACAATCCCTCCTTCACTTTTCAACAAGGAGTGCCATGGAGCAGGAAATTTGCTCGAGTTTGCGGGTATCAGAGCCGAGCAACTGAACAGGATTTCGACAAGAAATCCCTGCAACTTCACTAGAGTTTACAGAGGTAATCTCCAACCAACGTTTAACCATAATGGTTCTATGATTTTTCTTGATCTTTCGCATAACTCCTTGTCCGGTAGCATTCCCAAAGAGATTGGGAGCATGTACTATCTCTATATTTTGAATTTGGGCCATAACAATATATCTGGTTCAATCCCACAAGAGCTTGGAAAAATGACTAGCCTCAACATTCTTGATCTCTCTAGCAATAGCCTTGCGGGGACTATTCCACCGGCTCTGAGTGGCCTTACCTTGCTAACGGAGATTGATCTATCAAACAACCTTTTGTCTGGAATGATTCCAGAGTTGGGTCAATTTGAGACGTTCCCTGCCTACAGATTTGCCAACAATTCTGACCTATGTGGCTATCCTTTGGCTTCATGTGGGGGAGCTTTGGGGCCAAATGCAACTGCACATCAAAAGTCTCATCGACGAGAAGCATCCCTGGCGGGGAGTGTGGCAATGGGGTTACTCATCTCACTTTTCTGCATCTTTGGTTTGTTCATTGTTGCCATTGAAACCAAGAAAAGGCGGAAAAAAAAGGAATTGGCCCTTGATGTTCATATCGACAGTCTTAACCAATCAGGAACTGCCAATGGCTGGAAGCTAACAGGTGCCCGGGAAGCATTAAGCATCAACCTTGCGACATTTGAGAAGCCCCTTCAAAAGCTCACTTTTGCAGATCTTCTCGAGGCCACCAATGGTTTCCACGATAATAGCCTTATTGGCAAAGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAAGATGGCAGTGTCGTGGCCATAAAGAAACTAATACATATCAGCGGACAGGGTGATCGCGAATTCACTGCAGAAATGGAAACAATAGGGAAGATCAAGCACCGGAACCTTGTCCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTGGTTTATGAGTACATGAAATATGGAAGCTTAGATGATGTTTTACATGAACCGAAGAAAGCTGGCATCAAGTTGAACTGGGTTGCAAGGAGGAAGATTGCCATTGGGTCCGCGAGGGGACTGGCCTTTCTTCACCACAATTGCATCCCACACATCATTCACAGGGATATGAAATCGAGCAACGTGCTGGTGGATGAAAATTTGGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACCCATTTGAGTGTGAGCACTCTAGCAGGCACCCCTGGTTATGTCCCTCCTGAATACTACCAGAGCTTTAGATGTTCCACGAAAGGTGATGTTTATAGTTATGGAGTAGTATTGCTTGAGCTGCTAACGGGAAGACGTCCTACAGATTCAGCAGATTTTGGCGACAATAATCTCGTGGGTTGGGTAAAACAACACGCTAAATTGAAAATAAGTGATGTTTTTGATCCGGAGCTCATGAAAGAGGACGCAAGCCTTGAGATTGAGCTTTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGACGACAGGCCATGGCTGCGTCCGACAATGATCCAAGTGATGTTAAAGTTAAAGGAAATCCAAGCCGGGTCTGGGATGGACTCCCAATCAACGATAGCCACGGACGACGACGGAGTTTTTGGCGGAGTTGAAATGGTAGAGATGAGCATAAAAGAAGTCCCGGAAAGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1180

Amino Acids

127.74

Weight (kDa)

6.08

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 41 - 75 1.6e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 215 - 271 5.7e-06 Leucine rich repeat
LRR_8 PF13855 237 - 295 2.2e-06 Leucine rich repeat
LRR_14 PF23598 252 - 488 5e-09 Leucine-rich repeat region
LRR_8 PF13855 306 - 366 4e-07 Leucine rich repeat
LRR_14 PF23598 447 - 581 1.7e-06 Leucine-rich repeat region
LRR_8 PF13855 454 - 513 7.2e-06 Leucine rich repeat
LRR_8 PF13855 502 - 560 4.8e-07 Leucine rich repeat
LRR_8 PF13855 526 - 585 2.8e-07 Leucine rich repeat
Island PF20141 596 - 639 2.4e-20 Brassinosteroid receptor island domain
LRR_8 PF13855 668 - 726 9.7e-08 Leucine rich repeat
Pkinase PF00069 866 - 1135 7.7e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 870 - 1099 3.4e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
AatII GACGTC 2 cut(s) 456, 3202
Acc36I ACCTGC 1 cut(s) 710
AccB1I GGYRCC 3 cut(s) 1400, 2504, 3092
AccB7I CCANNNNNTGG 1 cut(s) 2490
AccI GTMKAC 1 cut(s) 2640
AccII CGCG 4 cut(s) 1208, 1440, 2715, 2921
AccIII TCCGGA 1 cut(s) 3289
AclI AACGTT 1 cut(s) 1910
AclWI GGATC 8 cut(s) 626, 1233, 1271, 1284, 1327, 1743, 3281, 3388
AcoI YGGCCR 2 cut(s) 900, 2673
AcsI RAATTY 4 cut(s) 1807, 2014, 2717, 3007
AcuI CTGAAG 2 cut(s) 1292, 1493
AcyI GRCGYC 2 cut(s) 453, 3199
AfaI GTAC 4 cut(s) 1304, 1496, 1997, 2822
AfiI CCNNNNNNNGG 8 cut(s) 764, 1706, 2198, 2340, 2418, 2490, 2777, 3435
AjnI CCWGG 3 cut(s) 233, 2334, 3097
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
Alw21I GWGCWC 6 cut(s) 720, 1005, 1055, 1707, 3083, 3297
Alw26I GTCTC 4 cut(s) 114, 2204, 2319, 3028
AlwI GGATC 8 cut(s) 626, 1233, 1271, 1284, 1327, 1743, 3281, 3388
AlwNI CAGNNNCTG 1 cut(s) 2480
Ama87I CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
Aor13HI TCCGGA 1 cut(s) 3289
ApeKI GCWGC 5 cut(s) 393, 705, 850, 3184, 3379
ApoI RAATTY 4 cut(s) 1807, 2014, 2717, 3007
ArsI GACNNNNNNTTYG 2 cut(s) 3464, 3496
Asp700I GAANNNNTTC 1 cut(s) 881
AsuC2I CCSGG 7 cut(s) 1626, 1627, 1700, 2509, 2510, 3430, 3524
AsuNHI GCTAGC 1 cut(s) 1651
AvaI CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
AvaII GGWCC 4 cut(s) 784, 890, 1178, 2916
BaeGI GKGCMC 2 cut(s) 1403, 2509
BalI TGGCCA 1 cut(s) 2675
BamHI GGATCC 1 cut(s) 1276
BanI GGYRCC 3 cut(s) 1400, 2504, 3092
BanII GRGCYC 5 cut(s) 1005, 1055, 1269, 1707, 3297
BarI GAAGNNNNNNTAC 2 cut(s) 3187, 3219
BauI CACGAG 1 cut(s) 3236
BbsI GAAGAC 1 cut(s) 3202
Bbv12I GWGCWC 6 cut(s) 720, 1005, 1055, 1707, 3083, 3297
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 405, 717, 862, 3171, 3366
BccI CCATC 4 cut(s) 541, 1412, 2654, 3433
BceAI ACGGC 1 cut(s) 2819
BcgI CGANNNNNNTGC 4 cut(s) 2705, 2739, 2974, 3008
BciT130I CCWGG 3 cut(s) 235, 2336, 3099
BciVI GTATCC 1 cut(s) 2774
BcnI CCSGG 7 cut(s) 1626, 1627, 1700, 2509, 2510, 3430, 3524
BcoDI GTCTC 4 cut(s) 114, 2204, 2319, 3028
BfaI CTAG 6 cut(s) 1281, 1652, 1881, 2070, 2094, 3086
BfmI CTRYAG 4 cut(s) 703, 2224, 2724, 3204
BfuAI ACCTGC 1 cut(s) 710
BfuI GTATCC 1 cut(s) 2774
BglI GCCNNNNNGGC 1 cut(s) 2616
BglII AGATCT 2 cut(s) 415, 2572
BisI GCNGC 7 cut(s) 394, 468, 471, 706, 851, 3185, 3380
BlsI GCNGC 7 cut(s) 395, 469, 472, 707, 852, 3186, 3381
Bme18I GGWCC 4 cut(s) 784, 890, 1178, 2916
BmeT110I CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
BmrI ACTGGG 1 cut(s) 2896
BmsI GCATC 6 cut(s) 1033, 2339, 2388, 2532, 2883, 2961
BmtI GCTAGC 1 cut(s) 1655
BmuI ACTGGG 1 cut(s) 2896
BoxI GACNNNNGTC 1 cut(s) 825
BpiI GAAGAC 1 cut(s) 3202
BplI GAGNNNNNCTC 2 cut(s) 1884, 1916
BpmI CTGGAG 1 cut(s) 734
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 3 cut(s) 1646, 3200, 3338
BpuMI CCSGG 7 cut(s) 1626, 1627, 1700, 2509, 2510, 3430, 3524
BsaBI GATNNNNATC 1 cut(s) 1553
BsaHI GRCGYC 2 cut(s) 453, 3199
BsaWI WCCGGW 3 cut(s) 1964, 2757, 3289
Bsc4I CCNNNNNNNGG 8 cut(s) 764, 1706, 2198, 2340, 2418, 2490, 2777, 3435
Bse118I RCCGGY 1 cut(s) 2122
Bse1I ACTGG 3 cut(s) 185, 2891, 2934
Bse3DI GCAATG 2 cut(s) 2358, 3063
Bse8I GATNNNNATC 1 cut(s) 1553
BseAI TCCGGA 1 cut(s) 3289
BseBI CCWGG 3 cut(s) 235, 2336, 3099
BseGI GGATG 6 cut(s) 533, 1404, 2330, 2952, 3007, 3444
BseJI GATNNNNATC 1 cut(s) 1553
BseLI CCNNNNNNNGG 8 cut(s) 764, 1706, 2198, 2340, 2418, 2490, 2777, 3435
BseMI GCAATG 2 cut(s) 2358, 3063
BseMII CTCAG 8 cut(s) 151, 868, 1040, 1243, 1373, 1457, 1681, 2120
BseNI ACTGG 3 cut(s) 185, 2891, 2934
BseSI GKGCMC 2 cut(s) 1403, 2509
BseXI GCAGC 5 cut(s) 405, 717, 862, 3171, 3366
BseYI CCCAGC 3 cut(s) 126, 1258, 1262
BsgI GTGCAG 4 cut(s) 412, 1008, 1555, 2286
Bsh1236I CGCG 4 cut(s) 1208, 1440, 2715, 2921
BshNI GGYRCC 3 cut(s) 1400, 2504, 3092
BsiHKAI GWGCWC 6 cut(s) 720, 1005, 1055, 1707, 3083, 3297
BsiHKCI CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
BslFI GGGAC 7 cut(s) 440, 916, 2125, 2753, 2940, 3092, 3506
BslI CCNNNNNNNGG 8 cut(s) 764, 1706, 2198, 2340, 2418, 2490, 2777, 3435
BsmAI GTCTC 4 cut(s) 114, 2204, 2319, 3028
BsmBI CGTCTC 1 cut(s) 2204
BsmFI GGGAC 7 cut(s) 440, 916, 2125, 2753, 2940, 3092, 3506
BsmI GAATGC 1 cut(s) 1971
BsoBI CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
Bsp1286I GDGCHC 9 cut(s) 720, 1005, 1055, 1269, 1403, 1707, 2509, 3083, 3297
Bsp13I TCCGGA 1 cut(s) 3289
Bsp19I CCATGG 2 cut(s) 1795, 3374
Bsp68I TCGCGA 3 cut(s) 1208, 1440, 2715
BspCNI CTCAG 8 cut(s) 150, 867, 1041, 1242, 1372, 1458, 1680, 2121
BspEI TCCGGA 1 cut(s) 3289
BspFNI CGCG 4 cut(s) 1208, 1440, 2715, 2921
BspHI TCATGA 2 cut(s) 303, 3297
BspMAI CTGCAG 2 cut(s) 707, 2728
BspMI ACCTGC 1 cut(s) 710
BspOI GCTAGC 1 cut(s) 1655
BspPI GGATC 8 cut(s) 626, 1233, 1271, 1284, 1327, 1743, 3281, 3388
BspT107I GGYRCC 3 cut(s) 1400, 2504, 3092
BsrDI GCAATG 2 cut(s) 2358, 3063
BsrFI RCCGGY 1 cut(s) 2122
BsrI ACTGG 3 cut(s) 185, 2891, 2934
BssAI RCCGGY 1 cut(s) 2122
BssNAI GTATAC 1 cut(s) 2641
BssNI GRCGYC 2 cut(s) 453, 3199
BssSI CACGAG 1 cut(s) 3236
BssT1I CCWWGG 5 cut(s) 934, 1498, 1795, 2776, 3374
Bst1107I GTATAC 1 cut(s) 2641
Bst2BI CACGAG 1 cut(s) 3236
Bst2UI CCWGG 3 cut(s) 235, 2336, 3099
Bst4CI ACNGT 6 cut(s) 286, 632, 890, 1355, 1715, 2462
Bst6I CTCTTC 2 cut(s) 68, 759
BstACI GRCGYC 2 cut(s) 453, 3199
BstC8I GCNNGC 9 cut(s) 475, 710, 848, 1206, 1653, 2872, 3091, 3313, 3533
BstDSI CCRYGG 3 cut(s) 1795, 3374, 3462
BstF5I GGATG 6 cut(s) 533, 1404, 2330, 2952, 3007, 3444
BstFNI CGCG 4 cut(s) 1208, 1440, 2715, 2921
BstMAI GTCTC 4 cut(s) 114, 2204, 2319, 3028
BstNI CCWGG 3 cut(s) 235, 2336, 3099
BstNSI RCATGY 1 cut(s) 1996
BstPAI GACNNNNGTC 1 cut(s) 825
BstSFI CTRYAG 4 cut(s) 703, 2224, 2724, 3204
BstSLI GKGCMC 2 cut(s) 1403, 2509
BstUI CGCG 4 cut(s) 1208, 1440, 2715, 2921
BstV1I GCAGC 5 cut(s) 405, 717, 862, 3171, 3366
BstV2I GAAGAC 1 cut(s) 3202
BstX2I RGATCY 6 cut(s) 415, 618, 1225, 1276, 1735, 2572
BstXI CCANNNNNNTGG 1 cut(s) 1469
BstYI RGATCY 6 cut(s) 415, 618, 1225, 1276, 1735, 2572
BstZ17I GTATAC 1 cut(s) 2641
BsuI GTATCC 1 cut(s) 2774
BtgI CCRYGG 3 cut(s) 1795, 3374, 3462
BtsCI GGATG 6 cut(s) 533, 1404, 2330, 2952, 3007, 3444
BtsI GCAGTG 2 cut(s) 2671, 2721
BtsIMutI CAGTG 3 cut(s) 1046, 2671, 2721
BtuMI TCGCGA 3 cut(s) 1208, 1440, 2715
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 9 cut(s) 475, 710, 848, 1206, 1653, 2872, 3091, 3313, 3533
CaiI CAGNNNCTG 1 cut(s) 2480
CciI TCATGA 2 cut(s) 303, 3297
Cfr10I RCCGGY 1 cut(s) 2122
Cfr9I CCCGGG 2 cut(s) 1625, 2508
CpoI CGGWCCG 1 cut(s) 890
CseI GACGC 2 cut(s) 3317, 3371
Csp6I GTAC 4 cut(s) 1303, 1495, 1996, 2821
CspCI CAANNNNNGTGG 2 cut(s) 1594, 1629
CspI CGGWCCG 1 cut(s) 890
CviQI GTAC 4 cut(s) 1303, 1495, 1996, 2821
EaeI YGGCCR 2 cut(s) 900, 2673
Eam1104I CTCTTC 2 cut(s) 68, 759
EarI CTCTTC 2 cut(s) 68, 759
EciI GGCGGA 5 cut(s) 87, 469, 532, 2437, 3501
Ecl136II GAGCTC 4 cut(s) 1003, 1053, 1705, 3295
Eco130I CCWWGG 5 cut(s) 934, 1498, 1795, 2776, 3374
Eco24I GRGCYC 5 cut(s) 1005, 1055, 1269, 1707, 3297
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 4 cut(s) 784, 890, 1178, 2916
Eco53kI GAGCTC 4 cut(s) 1003, 1053, 1705, 3295
Eco57I CTGAAG 2 cut(s) 1292, 1493
Eco88I CYCGRG 4 cut(s) 1625, 1814, 2508, 2579
EcoICRI GAGCTC 4 cut(s) 1003, 1053, 1705, 3295
EcoO109I RGGNCCY 1 cut(s) 599
EcoRI GAATTC 1 cut(s) 2717
EcoRII CCWGG 3 cut(s) 233, 2334, 3097
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 5 cut(s) 934, 1498, 1795, 2776, 3374
EcoT38I GRGCYC 5 cut(s) 1005, 1055, 1269, 1707, 3297
ErhI CCWWGG 5 cut(s) 934, 1498, 1795, 2776, 3374
Esp3I CGTCTC 1 cut(s) 2204
FalI AAGNNNNNCTT 2 cut(s) 2790, 2822
FaqI GGGAC 7 cut(s) 440, 916, 2125, 2753, 2940, 3092, 3506
FauI CCCGC 4 cut(s) 1265, 1816, 2101, 2332
FblI GTMKAC 1 cut(s) 2640
Fnu4HI GCNGC 7 cut(s) 394, 468, 471, 706, 851, 3185, 3380
FokI GGATG 6 cut(s) 520, 1391, 2317, 2939, 3014, 3451
FriOI GRGCYC 5 cut(s) 1005, 1055, 1269, 1707, 3297
Fsp4HI GCNGC 7 cut(s) 394, 468, 471, 706, 851, 3185, 3380
FspBI CTAG 6 cut(s) 1281, 1652, 1881, 2070, 2094, 3086
GluI GCNGC 7 cut(s) 394, 468, 471, 706, 851, 3185, 3380
GsaI CCCAGC 3 cut(s) 130, 1262, 1266
GsuI CTGGAG 1 cut(s) 734
HgaI GACGC 2 cut(s) 3317, 3371
Hin1I GRCGYC 2 cut(s) 453, 3199
HindIII AAGCTT 3 cut(s) 1433, 1640, 2836
Hpy166II GTNNAC 3 cut(s) 699, 1888, 2641
Hpy8I GTNNAC 3 cut(s) 699, 1888, 2641
Hpy99I CGWCG 6 cut(s) 611, 632, 890, 2325, 3473, 3476
HpyCH4III ACNGT 6 cut(s) 286, 632, 890, 1355, 1715, 2462
HpyCH4IV ACGT 7 cut(s) 296, 453, 606, 1910, 2213, 2991, 3199
HpySE526I ACGT 7 cut(s) 296, 453, 606, 1910, 2213, 2991, 3199
Hsp92I GRCGYC 2 cut(s) 453, 3199
Kpn2I TCCGGA 1 cut(s) 3289
Lsp1109I GCAGC 5 cut(s) 405, 717, 862, 3171, 3366
LweI GCATC 6 cut(s) 1033, 2339, 2388, 2532, 2883, 2961
MaeI CTAG 6 cut(s) 1281, 1652, 1881, 2070, 2094, 3086
MaeII ACGT 7 cut(s) 296, 453, 606, 1910, 2213, 2991, 3199
MaeIII GTNAC 4 cut(s) 638, 1670, 1709, 2358
MfeI CAATTG 2 cut(s) 2651, 2947
MflI RGATCY 6 cut(s) 415, 618, 1225, 1276, 1735, 2572
MhlI GDGCHC 9 cut(s) 720, 1005, 1055, 1269, 1403, 1707, 2509, 3083, 3297
MlsI TGGCCA 1 cut(s) 2675
MluNI TGGCCA 1 cut(s) 2675
MlyI GAGTC 3 cut(s) 308, 3030, 3437
Mox20I TGGCCA 1 cut(s) 2675
MroI TCCGGA 1 cut(s) 3289
MroXI GAANNNNTTC 1 cut(s) 881
MscI TGGCCA 1 cut(s) 2675
MseI TTAA 8 cut(s) 974, 1142, 1562, 1914, 2466, 2519, 3407, 3413
MslI CAYNNNNRTG 1 cut(s) 3072
Msp20I TGGCCA 1 cut(s) 2675
MspA1I CMGCKG 4 cut(s) 1262, 1290, 1465, 2700
MunI CAATTG 2 cut(s) 2651, 2947
Mva1269I GAATGC 1 cut(s) 1971
MvaI CCWGG 3 cut(s) 235, 2336, 3099
MvnI CGCG 4 cut(s) 1208, 1440, 2715, 2921
NciI CCSGG 7 cut(s) 1626, 1627, 1700, 2509, 2510, 3430, 3524
NcoI CCATGG 2 cut(s) 1795, 3374
NheI GCTAGC 1 cut(s) 1651
NmeAIII GCCGAG 2 cut(s) 755, 1861
NmuCI GTSAC 2 cut(s) 638, 1709
NruI TCGCGA 3 cut(s) 1208, 1440, 2715
NspI RCATGY 1 cut(s) 1996
PaeR7I CTCGAG 2 cut(s) 1814, 2579
PagI TCATGA 2 cut(s) 303, 3297
PaqCI CACCTGC 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 1337
PctI GAATGC 1 cut(s) 1971
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 8 cut(s) 323, 895, 980, 1070, 1513, 1549, 2188, 3209
PflMI CCANNNNNTGG 1 cut(s) 2490
PfoI TCCNGGA 1 cut(s) 3522
PkrI GCNGC 7 cut(s) 395, 469, 472, 707, 852, 3186, 3381
PleI GAGTC 3 cut(s) 308, 3029, 3437
PpsI GAGTC 3 cut(s) 308, 3029, 3437
PshAI GACNNNNGTC 1 cut(s) 825
Psp124BI GAGCTC 4 cut(s) 1005, 1055, 1707, 3297
Psp1406I AACGTT 1 cut(s) 1910
Psp6I CCWGG 3 cut(s) 233, 2334, 3097
PspFI CCCAGC 3 cut(s) 126, 1258, 1262
PspGI CCWGG 3 cut(s) 233, 2334, 3097
PspXI VCTCGAGB 1 cut(s) 1814
PstI CTGCAG 2 cut(s) 707, 2728
PstNI CAGNNNCTG 1 cut(s) 2480
PsuI RGATCY 6 cut(s) 415, 618, 1225, 1276, 1735, 2572
PvuII CAGCTG 3 cut(s) 1262, 1290, 1465
RruI TCGCGA 3 cut(s) 1208, 1440, 2715
RsaI GTAC 4 cut(s) 1304, 1496, 1997, 2822
RsaNI GTAC 4 cut(s) 1303, 1495, 1996, 2821
RseI CAYNNNNRTG 1 cut(s) 3072
Rsr2I CGGWCCG 1 cut(s) 890
RsrII CGGWCCG 1 cut(s) 890
SacI GAGCTC 4 cut(s) 1005, 1055, 1707, 3297
SaqAI TTAA 8 cut(s) 974, 1142, 1562, 1914, 2466, 2519, 3407, 3413
SatI GCNGC 7 cut(s) 394, 468, 471, 706, 851, 3185, 3380
SchI GAGTC 3 cut(s) 308, 3030, 3437
SduI GDGCHC 9 cut(s) 720, 1005, 1055, 1269, 1403, 1707, 2509, 3083, 3297
SfaNI GCATC 6 cut(s) 1033, 2339, 2388, 2532, 2883, 2961
SfcI CTRYAG 4 cut(s) 703, 2224, 2724, 3204
Sfr274I CTCGAG 2 cut(s) 1814, 2579
SinI GGWCC 4 cut(s) 784, 890, 1178, 2916
SlaI CTCGAG 2 cut(s) 1814, 2579
SmaI CCCGGG 2 cut(s) 1627, 2510
SmiMI CAYNNNNRTG 1 cut(s) 3072
SmlI CTYRAG 5 cut(s) 1661, 1814, 2579, 3179, 3317
SmoI CTYRAG 5 cut(s) 1661, 1814, 2579, 3179, 3317
SspMI CTAG 6 cut(s) 1281, 1652, 1881, 2070, 2094, 3086
SstI GAGCTC 4 cut(s) 1005, 1055, 1707, 3297
StyI CCWWGG 5 cut(s) 934, 1498, 1795, 2776, 3374
TaaI ACNGT 6 cut(s) 286, 632, 890, 1355, 1715, 2462
TaiI ACGT 7 cut(s) 299, 456, 609, 1913, 2216, 2994, 3202
TatI WGTACW 2 cut(s) 1995, 2820
TauI GCSGC 2 cut(s) 470, 473
TfiI GAWTC 8 cut(s) 323, 895, 980, 1070, 1513, 1549, 2188, 3209
Tru1I TTAA 8 cut(s) 974, 1142, 1562, 1914, 2466, 2519, 3407, 3413
Tru9I TTAA 8 cut(s) 974, 1142, 1562, 1914, 2466, 2519, 3407, 3413
TscAI CASTG 3 cut(s) 1053, 2671, 2728
TseFI GTSAC 2 cut(s) 638, 1709
TseI GCWGC 5 cut(s) 393, 705, 850, 3184, 3379
Tsp45I GTSAC 2 cut(s) 638, 1709
TspGWI ACGGA 6 cut(s) 602, 747, 1329, 2165, 3479, 3489
TspMI CCCGGG 2 cut(s) 1625, 2508
TspRI CASTG 3 cut(s) 1053, 2671, 2728
Van91I CCANNNNNTGG 1 cut(s) 2490
VpaK11BI GGWCC 4 cut(s) 784, 890, 1178, 2916
XapI RAATTY 4 cut(s) 1807, 2014, 2717, 3007
XceI RCATGY 1 cut(s) 1996
XcmI CCANNNNNNNNNTGG 2 cut(s) 2657, 3431
XhoI CTCGAG 2 cut(s) 1814, 2579
XmaI CCCGGG 2 cut(s) 1625, 2508
XmiI GTMKAC 1 cut(s) 2640
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 6 cut(s) 1281, 1652, 1881, 2070, 2094, 3086
ZraI GACGTC 2 cut(s) 454, 3200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.