RLG00000018379
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012455
Physical Location & Seq
Reverse (-)
28981878 .. 28985429
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018379

Sequence Viewer

Length: 3552 bp
ATGAAACCCCACAAACCCATTTCACACCACTTCTTTCTTCTTCTTCTTCTCTTCTTGCTCTCTGCAACTCCCTCTTCTTCTTATTCAGCTTACAAAGACTCCCAGCAACTCCTGAGCTTCAAAAACTCTCTTCCGAAACCTACCCTTCTCTCCGATTGGCTCCCTGACCACAACCCATGTCTGTTTTCCGGCGTTTTCTGCAAACAAACCCGGGTTTCCTCCATAGACCTGAGCTTCGTCACCTTGTCCACAAACCTCACAATTATCTCCACTTTCTTGATGACCATTGATTCTCTACAATCACTCACTCTGAAATCTACGGGCCTCACCGGCCCCATTTCTTTTCCGGCTAAATCCAAGTGCAGCCCTCTCCTCACTTACATAGATCTAGCTGAGAACAGCTTGTCCGGCCCAATTTCCGGTATTCCCAACTTGGCTTCCTGCTCGAGCTTGAACTCTCTTAACCTCTCCGCCAACCTCCTCGAGTTCAATGCCAAGGACTCGGCCACGTTCGGACTCTCCCTCCGGGTCCTCGACCTCTCCTTCAACAAGATTGCCGGCCCGGCCGTGCCGTGGATTGTATCGGACGGCTGCAGCGAGCTTGTAGAACTAGTTCTCAAGGGGAACAAGATCACCGGCGACATGAGTGTCTCTGCGTGCAAGAAGCTCAAGACTCTCGACTTCTCCTCCAACAATTTCACCGTGGAGATTCCGTCTTTCGGCGACTGTATGGTTCTGGACCATCTCGACATCTCCGGCAACAAATTCTCGGGCGACGTCGCGCATGCTCTCTCTTCCTGCAAGCAACTGACGTTTCTGAACCTCTCCAGAAACCATTTCTCCGGTCCTATTCCGGCCATTCCCACCCAGAATCTTCAGTTTCTTTCCCTCTCCGGCAATGAGTTCCAAGGTACAATTCCACCCAGCTTACTCAGTTCGTGTGAGTCGCTAGTAGAGCTGGATCTTTCCATGAATAATCTCTCGGGCACCGTTCCCGATGCGTTGAGCTCTTGCGCTTCCTTGGAGTCTCTTGACATATCCAACAACTTCTTCACCGGTGAGTTGCCCATCGAAACTCTGTTGAAGCTCAGCAACTTGAAGACTCTGTCTCTTTCTCTCAACGGTTTCTTTGGTACTCTGCCTCGCTCTCTCTCCAACCTTTCATATTTGGACAGCTTGGATCTGAGCTCGAACAATTTCACTGGCTCCATTCCGAGTTGGCTTTGCGAAGATCCCAGGAACAGCTGGAAGGAGCTGTACCTCCAGAACAATAAGTTCGGTGGTACTATTCCTCCAGCTCTCAGCAACTGTACTCAGCTCGTTGCTCTTGATTTGAGCTTCAATTACCTCACTGGTACCATCCCTGCTAGCTTGGGGTCTTTGTCTAAGCTTCGCGATTTGATCATTTGGTTGAATCAGCTTAGTGGGGAAATTCCCCAGGAGCTCATGAATCTTGGGTCGCTGGAGAATCTTATTCTGGACTTCAATGAGCTGACCGGGACGATTCCCGTGGGGCTGAGTAACTGCACCAATTTGAGCTGGATTTCGTTGGCGAATAACAAGTTGAGTGGTGAGATTCCGGCGTGGATTGGCAAGCTTCCGAAGCTGGCAATACTGAAGCTGAGTAACAACTCATTCTATGGGAACATTCCCCCGGAGCTGGGGGACTGTAAGAGCTTGATATGGTTGGATCTCAATACTAACTTGTTGAACGGTACAATCCCTCCTGGGCTTTTCAAACAGTCTGGCAACATTGCGGTGAATTTTGTTGCTTCCAAGACGTATGTGTATATCAAGAATGATGGAAGCAAGGAGTGCCATGGAGCAGGGAATTTGCTGGAGTTTGCGGGGATAAGACAAGAGCAATTGACCAGGATTTCGACGAGGAACCCCTGCAACTTCACTAGAGTGTATAGAGGTATTCTCCAACCAACATTTAATCACAATGGGACGATGATTTTTCTTGATATGTCACACAATAGGTTATCGGGTAGCATTCCGAAAGAGATTGGGAGTATGTACTATCTCTATATTCTGAATCTGGGCCATAATAATATATCTGGAGCAATCCCACTAGAGCTTGGGAAATTGAAGGACCTTAACATTCTTGATCTCTCTAGCAACAGCCTTGATGGAAGTATTCCTCAGTCTTTGGTCAGCCTTTCCATGCTGATGGAGATCGATTTGTCAAACAATCATCTGTCTGGAATGATTCCAGATTCAGGCCAGTTTGAGACATTCCCTGCTTACAGATTTATGAACAATTCAGGCCTTTGTGGCTACCCTTTAAATTCCTGTGGAGCTGCTTCTGGGGCAAATGGAAATGGGCACCAGAAATCTCATCGACAAGCGTCCCTGGCAGGCAGTGTGGCAATGGGGCTACTGTTTTCCCTTTTCTGCATCTTTGGTTTGATTATTGTTGCAATCGAAACCAAGAAAAGGCGAAAAAAGAAGGATTCAGGTCTGGACGTTTATGTGGACAGTCGTTCCCACTCCGGCACTGCCTGGAAGCTAACGGGTGCCCGAGAAGCATTAAGCATTAACCTCTCCACATTTGAGAAGCCACTTCAAAAGCTCACTTTTGCAGATCTTCTTGAGGCTACCCATGGTTTCCACAATGACAGTCTTATAGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAGGATGGCAGCATTGTTGCCATCAAGAAGTTGATACATATCAGCGGACAAGGTGATCGTGAATTCACTGCTGAAATGGAAACTATCGGGAAGATCAAGCACCGAAACCTTGTTCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTAGTTTACGAGTACATGAAGTATGGAAGCTTAGATGATGTTTTACATGACCAGAAGAAAGGTATCAAGCTGAGTTGGTCTGCAAGGAGGAAGATTGCGATTGGATCTGCGAGAGGACTGGCCTTTCTTCACCACAATTGCTTTCCTCACATCATTCATAGGGACATGAAATCGAGCAACGTCCTGCTCGATGAGAATCTAGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTATGAGTGCAATGGACACTCATTTGAGTGTGAGCACCCTGGCAGGCACACCTGGTTATGTTCCTCCAGAATACTACCAGAGTTTCAGATGTTCTACAAAAGGCGATGTTTATAGTTATGGTGTAGTATTGCTTGAGCTGCTAACAGGAAGACGTCCTACAGATTCAGCTGATTTTGGTGACAATAATCTTGTTGGTTGGGTGAAGCAACATGCTAAATTAAAAATAAGTGATGTTTTTGATCCAGAGCTCATGAAAGAGGACCCAACCCTTGAGATTGAGCTGTTACAGCACTTGAAGGTAGCTTGTGCTTGTTTGGATGATAGGCCATGGCGCCGCCCTACGATGATTCAAGTCATGGCGCAGTTCAAGGAAATCCAAGCAGGACCCGGGATGGACTCCCAATCAACCATAGCCACCGACGATGGAGGGTTTGGTGCGGTTGAAATGGTTGAGATGAGCATAAAAGAAGACCCTGAACCGGGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1184

Amino Acids

129.15

Weight (kDa)

6.21

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 67 1e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 222 - 280 6.6e-06 Leucine rich repeat
LRR_14 PF23598 236 - 472 1.1e-10 Leucine-rich repeat region
LRR_8 PF13855 291 - 349 6.4e-08 Leucine rich repeat
LRR_14 PF23598 432 - 566 6.8e-06 Leucine-rich repeat region
LRR_8 PF13855 487 - 545 1.7e-07 Leucine rich repeat
Island PF20141 583 - 647 8.7e-32 Brassinosteroid receptor island domain
LRR_14 PF23598 656 - 731 8.9e-06 Leucine-rich repeat region
LRR_8 PF13855 676 - 734 2.4e-06 Leucine rich repeat
Pkinase PF00069 871 - 1140 7.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 874 - 1140 9.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
AatII GACGTC 2 cut(s) 780, 3214
Acc65I GGTACC 1 cut(s) 1355
AccB1I GGYRCC 5 cut(s) 986, 1355, 2328, 2519, 3390
AccI GTMKAC 1 cut(s) 2655
AccII CGCG 2 cut(s) 782, 1395
AciI CCGC 6 cut(s) 471, 1757, 1847, 2715, 3394, 3497
AclWI GGATC 6 cut(s) 969, 1188, 1226, 1698, 2935, 3293
AcoI YGGCCR 3 cut(s) 504, 564, 855
AcsI RAATTY 6 cut(s) 764, 1431, 1762, 1831, 2290, 2732
AcuI CTGAAG 2 cut(s) 860, 1637
AcyI GRCGYC 3 cut(s) 777, 3211, 3391
AfaI GTAC 9 cut(s) 913, 1135, 1259, 1285, 1312, 1357, 1717, 2021, 2837
AgeI ACCGGT 1 cut(s) 1055
AhlI ACTAGT 1 cut(s) 610
AjnI CCWGG 8 cut(s) 1235, 1437, 1726, 1871, 2355, 2504, 3096, 3109
AjuI GAANNNNNNNTTGG 4 cut(s) 900, 932, 1034, 1066
AleI CACNNNNGTG 1 cut(s) 1907
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
Alw21I GWGCWC 5 cut(s) 1010, 1190, 1446, 3095, 3309
Alw26I GTCTC 5 cut(s) 655, 1032, 1113, 2228, 3040
AlwI GGATC 6 cut(s) 969, 1188, 1226, 1698, 2935, 3293
AlwNI CAGNNNCTG 1 cut(s) 1308
Ama87I CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
ApeKI GCWGC 6 cut(s) 363, 591, 594, 2303, 2679, 3196
ApoI RAATTY 6 cut(s) 764, 1431, 1762, 1831, 2290, 2732
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 1861, 1893
AsiGI ACCGGT 1 cut(s) 1055
Asp700I GAANNNNTTC 2 cut(s) 612, 1196
Asp718I GGTACC 1 cut(s) 1355
AspLEI GCGC 4 cut(s) 784, 1016, 3393, 3421
AsuC2I CCSGG 9 cut(s) 211, 212, 527, 563, 1498, 1655, 3447, 3448, 3540
AsuNHI GCTAGC 1 cut(s) 1367
AvaI CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
AvaII GGWCC 6 cut(s) 529, 739, 845, 2095, 3319, 3443
BaeGI GKGCMC 3 cut(s) 989, 2331, 2524
BanI GGYRCC 5 cut(s) 986, 1355, 2328, 2519, 3390
BanII GRGCYC 4 cut(s) 1010, 1190, 1446, 3309
BarI GAAGNNNNNNTAC 4 cut(s) 1241, 1273, 3199, 3231
BbsI GAAGAC 3 cut(s) 1106, 3214, 3534
Bbv12I GWGCWC 5 cut(s) 1010, 1190, 1446, 3095, 3309
BbvI GCAGC 6 cut(s) 375, 578, 606, 2290, 2691, 3183
BceAI ACGGC 4 cut(s) 551, 556, 604, 2834
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 8 cut(s) 1237, 1439, 1728, 1873, 2357, 2506, 3098, 3111
BciVI GTATCC 1 cut(s) 2789
BclI TGATCA 1 cut(s) 1401
BcnI CCSGG 9 cut(s) 211, 212, 527, 563, 1498, 1655, 3447, 3448, 3540
BcoDI GTCTC 5 cut(s) 655, 1032, 1113, 2228, 3040
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 8 cut(s) 389, 611, 950, 1368, 1905, 2075, 2118, 3023
BfmI CTRYAG 2 cut(s) 592, 3216
BfoI RGCGCY 1 cut(s) 3394
BfuI GTATCC 1 cut(s) 2789
BglI GCCNNNNNGGC 3 cut(s) 330, 563, 2277
BglII AGATCT 2 cut(s) 385, 2587
BisI GCNGC 7 cut(s) 364, 592, 595, 2304, 2680, 3197, 3394
BlpI GCTNAGC 1 cut(s) 1088
BlsI GCNGC 7 cut(s) 365, 593, 596, 2305, 2681, 3198, 3395
Bme18I GGWCC 6 cut(s) 529, 739, 845, 2095, 3319, 3443
BmeT110I CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
BmsI GCATC 2 cut(s) 988, 2409
BmtI GCTAGC 1 cut(s) 1371
BoxI GACNNNNGTC 1 cut(s) 2350
BpiI GAAGAC 3 cut(s) 1106, 3214, 3534
BplI GAGNNNNNCTC 2 cut(s) 1908, 1940
BpmI CTGGAG 7 cut(s) 811, 1247, 1278, 1484, 1859, 2082, 3108
Bpu10I CCTNAGC 2 cut(s) 113, 230
Bpu1102I GCTNAGC 1 cut(s) 1088
BpuEI CTTGAG 5 cut(s) 602, 653, 2615, 3212, 3350
BpuMI CCSGG 9 cut(s) 211, 212, 527, 563, 1498, 1655, 3447, 3448, 3540
Bsa29I ATCGAT 1 cut(s) 2181
BsaBI GATNNNNATC 3 cut(s) 2177, 2708, 3018
BsaHI GRCGYC 3 cut(s) 777, 3211, 3391
BsaWI WCCGGW 3 cut(s) 419, 842, 1055
Bse118I RCCGGY 4 cut(s) 329, 557, 635, 1055
Bse1I ACTGG 4 cut(s) 1207, 1357, 2227, 2946
Bse3DI GCAATG 4 cut(s) 904, 1752, 2379, 3075
Bse8I GATNNNNATC 3 cut(s) 2177, 2708, 3018
BseBI CCWGG 8 cut(s) 1237, 1439, 1728, 1873, 2357, 2506, 3098, 3111
BseCI ATCGAT 1 cut(s) 2181
BseGI GGATG 4 cut(s) 1359, 2680, 3382, 3456
BseJI GATNNNNATC 3 cut(s) 2177, 2708, 3018
BseMI GCAATG 4 cut(s) 904, 1752, 2379, 3075
BseNI ACTGG 4 cut(s) 1207, 1357, 2227, 2946
BseRI GAGGAG 3 cut(s) 362, 470, 676
BseSI GKGCMC 3 cut(s) 989, 2331, 2524
BseX3I CGGCCG 1 cut(s) 564
BseXI GCAGC 6 cut(s) 375, 578, 606, 2290, 2691, 3183
BseYI CCCAGC 3 cut(s) 102, 923, 1660
BsgI GTGCAG 2 cut(s) 382, 1510
Bsh1236I CGCG 2 cut(s) 782, 1395
Bsh1285I CGRYCG 1 cut(s) 567
BshNI GGYRCC 5 cut(s) 986, 1355, 2328, 2519, 3390
BshTI ACCGGT 1 cut(s) 1055
BshVI ATCGAT 1 cut(s) 2181
BsiEI CGRYCG 1 cut(s) 567
BsiHKAI GWGCWC 5 cut(s) 1010, 1190, 1446, 3095, 3309
BsiHKCI CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
BslFI GGGAC 5 cut(s) 1513, 1679, 1963, 2338, 2999
BsmAI GTCTC 5 cut(s) 655, 1032, 1113, 2228, 3040
BsmFI GGGAC 5 cut(s) 1513, 1679, 1963, 2338, 2999
BsmI GAATGC 1 cut(s) 1995
BsoBI CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
Bsp1286I GDGCHC 8 cut(s) 989, 1010, 1190, 1446, 2331, 2524, 3095, 3309
Bsp1720I GCTNAGC 1 cut(s) 1088
Bsp19I CCATGG 3 cut(s) 1819, 2605, 3386
Bsp68I TCGCGA 1 cut(s) 1395
BspACI CCGC 6 cut(s) 471, 1757, 1847, 2715, 3394, 3497
BspDI ATCGAT 1 cut(s) 2181
BspFNI CGCG 2 cut(s) 782, 1395
BspHI TCATGA 2 cut(s) 1446, 3309
BspMAI CTGCAG 1 cut(s) 596
BspOI GCTAGC 1 cut(s) 1371
BspPI GGATC 6 cut(s) 969, 1188, 1226, 1698, 2935, 3293
BspT107I GGYRCC 5 cut(s) 986, 1355, 2328, 2519, 3390
BsrDI GCAATG 4 cut(s) 904, 1752, 2379, 3075
BsrFI RCCGGY 4 cut(s) 329, 557, 635, 1055
BsrI ACTGG 4 cut(s) 1207, 1357, 2227, 2946
BssAI RCCGGY 4 cut(s) 329, 557, 635, 1055
BssNAI GTATAC 1 cut(s) 2656
BssNI GRCGYC 3 cut(s) 777, 3211, 3391
BssT1I CCWWGG 7 cut(s) 495, 907, 1020, 1819, 2605, 2791, 3386
Bst1107I GTATAC 1 cut(s) 2656
Bst2UI CCWGG 8 cut(s) 1237, 1439, 1728, 1873, 2357, 2506, 3098, 3111
Bst6I CTCTTC 4 cut(s) 56, 79, 135, 799
BstACI GRCGYC 3 cut(s) 777, 3211, 3391
BstAPI GCANNNNNTGC 1 cut(s) 1815
BstDSI CCRYGG 6 cut(s) 572, 702, 1509, 1819, 2605, 3386
BstF5I GGATG 4 cut(s) 1359, 2680, 3382, 3456
BstFNI CGCG 2 cut(s) 782, 1395
BstH2I RGCGCY 1 cut(s) 3394
BstHHI GCGC 4 cut(s) 784, 1016, 3393, 3421
BstMAI GTCTC 5 cut(s) 655, 1032, 1113, 2228, 3040
BstMCI CGRYCG 1 cut(s) 567
BstNI CCWGG 8 cut(s) 1237, 1439, 1728, 1873, 2357, 2506, 3098, 3111
BstNSI RCATGY 2 cut(s) 788, 3272
BstPAI GACNNNNGTC 1 cut(s) 2350
BstSFI CTRYAG 2 cut(s) 592, 3216
BstSLI GKGCMC 3 cut(s) 989, 2331, 2524
BstUI CGCG 2 cut(s) 782, 1395
BstV1I GCAGC 6 cut(s) 375, 578, 606, 2290, 2691, 3183
BstV2I GAAGAC 3 cut(s) 1106, 3214, 3534
BstX2I RGATCY 7 cut(s) 385, 961, 1180, 1231, 1690, 2587, 2927
BstXI CCANNNNNNTGG 1 cut(s) 2173
BstYI RGATCY 7 cut(s) 385, 961, 1180, 1231, 1690, 2587, 2927
BstZ17I GTATAC 1 cut(s) 2656
BstZI CGGCCG 1 cut(s) 564
Bsu15I ATCGAT 1 cut(s) 2181
BsuI GTATCC 1 cut(s) 2789
BsuTUI ATCGAT 1 cut(s) 2181
BtgI CCRYGG 6 cut(s) 572, 702, 1509, 1819, 2605, 3386
BtgZI GCGATG 1 cut(s) 3177
BtsCI GGATG 4 cut(s) 1359, 2680, 3382, 3456
BtsI GCAGTG 3 cut(s) 2371, 2499, 2736
BtsIMutI CAGTG 5 cut(s) 1200, 1350, 2371, 2499, 2736
BtuMI TCGCGA 1 cut(s) 1395
CaiI CAGNNNCTG 1 cut(s) 1308
CciI TCATGA 2 cut(s) 1446, 3309
CfoI GCGC 4 cut(s) 784, 1016, 3393, 3421
Cfr10I RCCGGY 4 cut(s) 329, 557, 635, 1055
Cfr9I CCCGGG 2 cut(s) 210, 3446
ClaI ATCGAT 1 cut(s) 2181
CseI GACGC 1 cut(s) 2340
CsiI ACCWGGT 1 cut(s) 3109
Csp6I GTAC 9 cut(s) 912, 1134, 1258, 1284, 1311, 1356, 1716, 2020, 2836
CspAI ACCGGT 1 cut(s) 1055
CspCI CAANNNNNGTGG 2 cut(s) 1549, 1584
CviQI GTAC 9 cut(s) 912, 1134, 1258, 1284, 1311, 1356, 1716, 2020, 2836
DinI GGCGCC 1 cut(s) 3392
DraI TTTAAA 1 cut(s) 2289
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
EaeI YGGCCR 3 cut(s) 504, 564, 855
EagI CGGCCG 1 cut(s) 564
Eam1104I CTCTTC 4 cut(s) 56, 79, 135, 799
EarI CTCTTC 4 cut(s) 56, 79, 135, 799
EciI GGCGGA 1 cut(s) 460
Ecl136II GAGCTC 4 cut(s) 1008, 1188, 1444, 3307
EclXI CGGCCG 1 cut(s) 564
Eco130I CCWWGG 7 cut(s) 495, 907, 1020, 1819, 2605, 2791, 3386
Eco147I AGGCCT 1 cut(s) 2271
Eco24I GRGCYC 4 cut(s) 1010, 1190, 1446, 3309
Eco47I GGWCC 6 cut(s) 529, 739, 845, 2095, 3319, 3443
Eco52I CGGCCG 1 cut(s) 564
Eco53kI GAGCTC 4 cut(s) 1008, 1188, 1444, 3307
Eco57I CTGAAG 2 cut(s) 860, 1637
Eco88I CYCGRG 7 cut(s) 210, 445, 482, 769, 982, 2523, 3446
EcoICRI GAGCTC 4 cut(s) 1008, 1188, 1444, 3307
EcoO109I RGGNCCY 4 cut(s) 529, 2095, 3319, 3443
EcoRI GAATTC 1 cut(s) 2732
EcoRII CCWGG 8 cut(s) 1235, 1437, 1726, 1871, 2355, 2504, 3096, 3109
EcoT14I CCWWGG 7 cut(s) 495, 907, 1020, 1819, 2605, 2791, 3386
EcoT38I GRGCYC 4 cut(s) 1010, 1190, 1446, 3309
EgeI GGCGCC 1 cut(s) 3392
EheI GGCGCC 1 cut(s) 3392
ErhI CCWWGG 7 cut(s) 495, 907, 1020, 1819, 2605, 2791, 3386
FalI AAGNNNNNCTT 2 cut(s) 2805, 2837
FaqI GGGAC 5 cut(s) 1513, 1679, 1963, 2338, 2999
FauI CCCGC 1 cut(s) 1840
FbaI TGATCA 1 cut(s) 1401
FblI GTMKAC 1 cut(s) 2655
Fnu4HI GCNGC 7 cut(s) 364, 592, 595, 2304, 2680, 3197, 3394
FokI GGATG 4 cut(s) 1346, 2687, 3389, 3463
FriOI GRGCYC 4 cut(s) 1010, 1190, 1446, 3309
Fsp4HI GCNGC 7 cut(s) 364, 592, 595, 2304, 2680, 3197, 3394
FspBI CTAG 8 cut(s) 389, 611, 950, 1368, 1905, 2075, 2118, 3023
GlaI GCGC 4 cut(s) 783, 1015, 3392, 3420
GluI GCNGC 7 cut(s) 364, 592, 595, 2304, 2680, 3197, 3394
GsaI CCCAGC 3 cut(s) 106, 927, 1664
GsuI CTGGAG 7 cut(s) 811, 1247, 1278, 1484, 1859, 2082, 3108
HaeII RGCGCY 1 cut(s) 3394
HgaI GACGC 1 cut(s) 2340
HhaI GCGC 4 cut(s) 784, 1016, 3393, 3421
Hin1I GRCGYC 3 cut(s) 777, 3211, 3391
Hin6I GCGC 4 cut(s) 782, 1014, 3391, 3419
HinP1I GCGC 4 cut(s) 782, 1014, 3391, 3419
HindIII AAGCTT 3 cut(s) 1388, 1595, 2851
Hpy166II GTNNAC 4 cut(s) 249, 2479, 2656, 2830
Hpy8I GTNNAC 4 cut(s) 249, 2479, 2656, 2830
Hpy99I CGWCG 4 cut(s) 779, 782, 1885, 3482
HpyAV CCTTC 7 cut(s) 155, 553, 1243, 2086, 2446, 2665, 3349
HpyCH4IV ACGT 7 cut(s) 509, 777, 812, 1781, 2469, 3003, 3211
HpySE526I ACGT 7 cut(s) 509, 777, 812, 1781, 2469, 3003, 3211
Hsp92I GRCGYC 3 cut(s) 777, 3211, 3391
HspAI GCGC 4 cut(s) 782, 1014, 3391, 3419
KasI GGCGCC 1 cut(s) 3390
KpnI GGTACC 1 cut(s) 1359
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 1401
LmnI GCTCC 8 cut(s) 165, 1211, 1252, 1441, 1657, 1823, 2063, 2300
Lsp1109I GCAGC 6 cut(s) 375, 578, 606, 2290, 2691, 3183
LweI GCATC 2 cut(s) 988, 2409
MabI ACCWGGT 1 cut(s) 3109
MaeI CTAG 8 cut(s) 389, 611, 950, 1368, 1905, 2075, 2118, 3023
MaeII ACGT 7 cut(s) 509, 777, 812, 1781, 2469, 3003, 3211
MaeIII GTNAC 6 cut(s) 238, 1520, 1625, 1971, 3236, 3342
MfeI CAATTG 3 cut(s) 1865, 2666, 2959
MflI RGATCY 7 cut(s) 385, 961, 1180, 1231, 1690, 2587, 2927
MhlI GDGCHC 8 cut(s) 989, 1010, 1190, 1446, 2331, 2524, 3095, 3309
Mly113I GGCGCC 1 cut(s) 3391
MlyI GAGTC 9 cut(s) 92, 494, 510, 667, 953, 1034, 1096, 3042, 3449
MmeI TCCRAC 5 cut(s) 714, 1065, 1179, 1668, 1951
MroNI GCCGGC 1 cut(s) 557
MroXI GAANNNNTTC 2 cut(s) 612, 1196
MseI TTAA 7 cut(s) 462, 1938, 2100, 2288, 2534, 2541, 3278
MslI CAYNNNNRTG 4 cut(s) 1757, 1907, 2171, 3084
MspA1I CMGCKG 3 cut(s) 1245, 2715, 3227
MunI CAATTG 3 cut(s) 1865, 2666, 2959
Mva1269I GAATGC 1 cut(s) 1995
MvaI CCWGG 8 cut(s) 1237, 1439, 1728, 1873, 2357, 2506, 3098, 3111
MvnI CGCG 2 cut(s) 782, 1395
NaeI GCCGGC 1 cut(s) 559
NarI GGCGCC 1 cut(s) 3391
NciI CCSGG 9 cut(s) 211, 212, 527, 563, 1498, 1655, 3447, 3448, 3540
NcoI CCATGG 3 cut(s) 1819, 2605, 3386
NgoMIV GCCGGC 1 cut(s) 557
NheI GCTAGC 1 cut(s) 1367
NmeAIII GCCGAG 1 cut(s) 482
NmuCI GTSAC 3 cut(s) 238, 1971, 3236
NruI TCGCGA 1 cut(s) 1395
NspI RCATGY 2 cut(s) 788, 3272
OliI CACNNNNGTG 1 cut(s) 1907
PaeI GCATGC 1 cut(s) 788
PaeR7I CTCGAG 2 cut(s) 445, 482
PagI TCATGA 2 cut(s) 1446, 3309
PceI AGGCCT 1 cut(s) 2271
PcsI WCGNNNNNNNCGW 2 cut(s) 594, 3009
PctI GAATGC 1 cut(s) 1995
PdiI GCCGGC 1 cut(s) 559
PdmI GAANNNNTTC 2 cut(s) 612, 1196
PflFI GACNNNGTC 1 cut(s) 1105
PinAI ACCGGT 1 cut(s) 1055
PkrI GCNGC 7 cut(s) 365, 593, 596, 2305, 2681, 3198, 3395
PleI GAGTC 9 cut(s) 92, 494, 510, 667, 952, 1033, 1096, 3041, 3449
PluTI GGCGCC 1 cut(s) 3394
PpsI GAGTC 9 cut(s) 92, 494, 510, 667, 952, 1033, 1096, 3041, 3449
PpuMI RGGWCCY 4 cut(s) 529, 2095, 3319, 3443
PshAI GACNNNNGTC 1 cut(s) 2350
Psp124BI GAGCTC 4 cut(s) 1010, 1190, 1446, 3309
Psp5II RGGWCCY 4 cut(s) 529, 2095, 3319, 3443
Psp6I CCWGG 8 cut(s) 1235, 1437, 1726, 1871, 2355, 2504, 3096, 3109
PspFI CCCAGC 3 cut(s) 102, 923, 1660
PspGI CCWGG 8 cut(s) 1235, 1437, 1726, 1871, 2355, 2504, 3096, 3109
PspPPI RGGWCCY 4 cut(s) 529, 2095, 3319, 3443
PspXI VCTCGAGB 2 cut(s) 445, 482
PsrI GAACNNNNNNTAC 2 cut(s) 597, 629
PstI CTGCAG 1 cut(s) 596
PstNI CAGNNNCTG 1 cut(s) 1308
PsuI RGATCY 7 cut(s) 385, 961, 1180, 1231, 1690, 2587, 2927
PsyI GACNNNGTC 1 cut(s) 1105
PvuII CAGCTG 2 cut(s) 1245, 3227
RruI TCGCGA 1 cut(s) 1395
RsaI GTAC 9 cut(s) 913, 1135, 1259, 1285, 1312, 1357, 1717, 2021, 2837
RsaNI GTAC 9 cut(s) 912, 1134, 1258, 1284, 1311, 1356, 1716, 2020, 2836
RseI CAYNNNNRTG 4 cut(s) 1757, 1907, 2171, 3084
SacI GAGCTC 4 cut(s) 1010, 1190, 1446, 3309
SaqAI TTAA 7 cut(s) 462, 1938, 2100, 2288, 2534, 2541, 3278
SatI GCNGC 7 cut(s) 364, 592, 595, 2304, 2680, 3197, 3394
SchI GAGTC 9 cut(s) 92, 494, 510, 667, 953, 1034, 1096, 3042, 3449
SduI GDGCHC 8 cut(s) 989, 1010, 1190, 1446, 2331, 2524, 3095, 3309
SexAI ACCWGGT 1 cut(s) 3109
SfaNI GCATC 2 cut(s) 988, 2409
SfcI CTRYAG 2 cut(s) 592, 3216
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SfoI GGCGCC 1 cut(s) 3392
Sfr274I CTCGAG 2 cut(s) 445, 482
SgrAI CRCCGGYG 2 cut(s) 635, 1055
SinI GGWCC 6 cut(s) 529, 739, 845, 2095, 3319, 3443
SlaI CTCGAG 2 cut(s) 445, 482
SmaI CCCGGG 2 cut(s) 212, 3448
SmiMI CAYNNNNRTG 4 cut(s) 1757, 1907, 2171, 3084
SmlI CTYRAG 7 cut(s) 445, 482, 617, 668, 2594, 3191, 3329
SmoI CTYRAG 7 cut(s) 445, 482, 617, 668, 2594, 3191, 3329
SpeI ACTAGT 1 cut(s) 610
SphI GCATGC 1 cut(s) 788
SseBI AGGCCT 1 cut(s) 2271
SsiI CCGC 6 cut(s) 471, 1757, 1847, 2715, 3394, 3497
SspDI GGCGCC 1 cut(s) 3390
SspMI CTAG 8 cut(s) 389, 611, 950, 1368, 1905, 2075, 2118, 3023
SstI GAGCTC 4 cut(s) 1010, 1190, 1446, 3309
StuI AGGCCT 1 cut(s) 2271
StyI CCWWGG 7 cut(s) 495, 907, 1020, 1819, 2605, 2791, 3386
TaiI ACGT 7 cut(s) 512, 780, 815, 1784, 2472, 3006, 3214
TatI WGTACW 3 cut(s) 1310, 2019, 2835
TauI GCSGC 1 cut(s) 3396
Tru1I TTAA 7 cut(s) 462, 1938, 2100, 2288, 2534, 2541, 3278
Tru9I TTAA 7 cut(s) 462, 1938, 2100, 2288, 2534, 2541, 3278
TscAI CASTG 5 cut(s) 1207, 1357, 2371, 2506, 2743
TseFI GTSAC 3 cut(s) 238, 1971, 3236
TseI GCWGC 6 cut(s) 363, 591, 594, 2303, 2679, 3196
Tsp45I GTSAC 3 cut(s) 238, 1971, 3236
TspDTI ATGAA 9 cut(s) 17, 986, 1152, 1463, 2273, 2855, 2969, 3005, 3326
TspGWI ACGGA 1 cut(s) 702
TspMI CCCGGG 2 cut(s) 210, 3446
TspRI CASTG 5 cut(s) 1207, 1357, 2371, 2506, 2743
Tth111I GACNNNGTC 1 cut(s) 1105
VpaK11BI GGWCC 6 cut(s) 529, 739, 845, 2095, 3319, 3443
XapI RAATTY 6 cut(s) 764, 1431, 1762, 1831, 2290, 2732
XbaI TCTAGA 1 cut(s) 3022
XceI RCATGY 2 cut(s) 788, 3272
XcmI CCANNNNNNNNNTGG 1 cut(s) 2672
XhoI CTCGAG 2 cut(s) 445, 482
XmaI CCCGGG 2 cut(s) 210, 3446
XmiI GTMKAC 1 cut(s) 2655
XmnI GAANNNNTTC 2 cut(s) 612, 1196
XspI CTAG 8 cut(s) 389, 611, 950, 1368, 1905, 2075, 2118, 3023
ZraI GACGTC 2 cut(s) 778, 3212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.