Rh2DG278500
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012455
Physical Location & Seq
Reverse (-)
31556575 .. 31561161
4587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG278500.1

Sequence Viewer

Length: 3549 bp
ATGAAACCCCACAAACCCATTTCACACCACTTCTTTCTTCTCCTCCTCTTCTTGCTCTCTGCAACTCCCTCTTCTTCCTCTTCAGCTTACAAAGACGCCCAGCAACTCCTGAGCTTCAAAAACTCTCTTCCGAAACCTACCGTTCTCTCCGATTGGCTCCCTGACCAAAACCCATGTCTGTTTTACGGCGTTTTCTGCAAACAAACCCGGGTTTCCTCCATAGACCTGAGCTCCGTCAACTTGTCCACAAACCTCACAATTATCTCCACTTTCTTGATGACCATTGATTCTCTACAATCACTCACTCTGAAATCTACCGGCCTCACCGGCCCCATTTCTTTTCCGGCTAAATCCAAGTGCAGCCCTCTCCTCACTTACATAGATCTAGCTGAGAACAGCTTGTCCGGCCCAATTTCCGGTATTCCCAGCTTGGCCTCCTGCTCGAGCTTGAACTCTCTTAACCTCTCCGCCAACCTCCTCGAGTTCAATGCCAAGGACTCGGCCACGTTCGGACTCTCCCTCCAGGTCCTCGACCTCTCCTTCAACAAGATTGCCGGCCCCGCCGTGCCGTGGATCGTATCCGACGGCTGCAGCGAGCTTGTAAAACTAGTTCTCAAGGGGAACAAGATCACCGGCGACATGAGTGTCTCTGGGTGCAAGAAGCTCGAGACCCTCGACTTCTCCTCCAACAATTTCACGGTGGAGATTCCGTCTTTCGGCGACTGTATGGTTCTGGGCCATCTCGACATCTCCGGCAACAAATTCTCCGGCGACGTCGCGCATGCTCTCTCTTCCTGCACGCAACTGACGTTTCTGAACCTCTCCAGAAACCATTTCTCCGGTCCCATTCCGGCCATTCCCAGCCAGAATCTTCAGTTTCTTTCCCTCTCCGGCAATGGGTTCCGAGGTACAATTCCACCCAGCTTGCTCAGTTCGTGTGAGTCGCTAGTGGAGCTGGATCTTTCCATAAATAATATCTCGGGCACCGTTCCGGATGCATTGAGCTCTTGCGCTTCCTTGGAGTCTCTCGACATCTCCAACAACTTCTTCACCGGTGAGTTGCCCATCGAAACTCTGTTGAAGCTCAGCAGCCTGAAGAGTGTGTCTCTTTCTCTCAACGGTTTCTCTGGTACTCTGCCTCGCTCTCTCTCCAAACTTTCATATCTAGACAGCTTGGATCTGAGCTCGAACAATTTCACCGGCTCCATTCCGAGTTGGCTTTGCGAAGATCCCAGGAACAGCTGGAAGGAGCTGTACCTCCAGAACAATAAGTTCGGTGGTACTATTCCTCCAGCTCTCAGCAACTGCACTCAGCTCGTGGCTCTTGATTTGAGCTTCAATTACCTCACCGGTACCATCCCTGCTAGCTTGGGGTCTTTGTCTAAGCTTCGCGACTTGATCATTTGGTTGAATCAGCTTAGCGGGGAAATTCCCCAGGAGCTCACGAATCTTGGGTTGCTGGAGAATCTTATCCTGGACTTCAATGAGCTGACCGGGACGATTCCTGTGGGGCTGAGTAACTGCACCAATTTGAGCTGGATTTCGTTGGCGAATAACAAGTTGAGTGGTGAGATTCCGGCGTGGATTGGCAAGCTTCCGAAGCTGGCAATACTGAAGCTGAGTAACAACTCATTCTATGGGAACATTCCCCCGGAGCTGGGGGACTGTAAGAGCTTGATATGGTTGGATCTCAATACTAACTTGCTGAATGGTACAATCCCTCCTGGGCTTTTCAAACAGTCTGGCAACATTGCGGTGAATTTTGTTGCTTCCAAGACGTATGTGTATATCAAGAATGATGGAAGCAAGGAGTGCCATGGAGCAGGGAATTTGCTCGAGTTTGCGGGGATAAGACAAGAGCAATTGACCAGGATTTCGACCAGGAACCCCTGCAACTTCACTAGAGTGTATAGAGGTATTCTCCAACCAACATTTAATCACAATGGGACCATGATTTTTCTTGATATGTCACACAATAGGTTATCGGGTAGCATTCCGAAAGAGATTGGGAGTATGTACTATCTCTATATTCTGAATCTGGGCCATAATAATATATCTGGAGCAATCCCGCTAGAGCTTGGGAAATTGAAGGACCTTAACATTCTTGATCTCTCTAGCAACAGCCTTGATGGAAGTATTCCTCAGTCTTTGGTCAGCCTTTCCATGCTGATGGAGATCGATTTGTCAAACAATCATCTATCTGGAATGATTCCAGATTCAGGCCAGTTTGAGACATTCCCTGCTTACAGATTTATGAACAATTCAGGCCTTTGTGGCTACCCTTTAAATTCCTGTGGAGCTGCTTCTGGGGCAAATGGAAATGGGCACCAGAAATCTCATCGACAAGCGTCCCTGGCAGGCAGTGTGGCAATGGGGCTACTGTTTTCCCTTTTCTGCATCTTTGGTTTGCTTATTGTTGCAATTGAAACCAGGAAAAGGCGAAAAAAGAAGGATTCAGGTCTGGACGTTTATGTGGACAGTCGTTCCCACTCCGGTACTGCCTGGAAGCTAACGGGTGCCCGAGAAGCATTAAGCATTAACCTCTCCACATTTGAGAAGCCACTTCAAAAGCTCACTTTTGCAGATCTTCTTGAGGCTACCAATGGTTTCCACAATGACAGTCTTATAGGCTCTGGTGGTTTTGGTGATGTATACAAGGCCCAATTGAAGGATGGCAGCATTGTTGCCATCAAGAAGTTGATACATATCAGTGGACAAGGTGATCGTGAATTCACTGCTGAAATGGAAACTATCGGGAAGATCAAGCACCGAAACCTTGTTCCCCTCCTTGGATACTGCAAAGTAGGAGAAGAACGGCTTTTAGTTTACGAGTACATGAAGTATGGAAGCTTAGATGATGTTTTACATGACCAGAAGAAAGGTATCAAGCTGAGTTGGTCTGCAAGGAGGAAGATTGCGATTGGATCTGCGAGAGGACTGGCCTTTCTTCACCACAATTGCATTCCTCACATCATTCATAGGGACATGAAATCGAGCAACGTCCTGCTCGATGAGAATCTAGAAGCCAGAGTCTCCGATTTTGGAATGGCAAGGCTTGTGAGTGCAATGGACACTCATTTGAGTGTGAGCACCCTGGCAGGCACACCTGGTTATGTTCCTCCAGAATACTACCAGAGTTTCAGATGTTCTACAAAAGGCGATGTTTATAGTTATGGTGTAGTATTGCTCGAGCTGCTAACAGGAAGACGTCCTACAGATTCAGCTGATTTTGGTGACAATAATCTTGTTGGTTGGGTGAAGCAACATGCTAAATTAAAAATAAGTGATGTTTTTGATCCAGAGCTCATGAAAGAGGACCCAACCCTTGAGATTGAGCTGTTACAGCACTTAAAGGTAGCTTGCGCTTGTTTGGATGATAGGCCATGGCGCCGCCCTACGATGATTCAAGTCATGGCGCAGTTCAAGGAAATCCAAGCAGGACCCGGGATGGACTCCCAATCAACCATAGCCACCGACGATGGAGGTTTTGGTGCAGTTGAAATGGTTGAGATGAGCATAAAAGAAGACCCTGAACCGGGCAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0001578 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009314 GO:0009411 GO:0009416 GO:0009555 GO:0009593 GO:0009628 GO:0009642 GO:0009646 GO:0009647 GO:0009653 GO:0009719 GO:0009720 GO:0009725 GO:0009726 GO:0009729 GO:0009741 GO:0009742 GO:0009755 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0009987 GO:0010008 GO:0010033 GO:0010208 GO:0010224 GO:0010268 GO:0010584 GO:0010927 GO:0010941 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022607 GO:0023052 GO:0030154 GO:0030198 GO:0031090 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0036211 GO:0042221 GO:0042592 GO:0042802 GO:0042803 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043401 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046982 GO:0046983 GO:0048229 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048518 GO:0048519 GO:0048523 GO:0048545 GO:0048580 GO:0048582 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051239 GO:0051240 GO:0051606 GO:0051716 GO:0055088 GO:0060548 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:1905393 GO:2000026 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1182

Amino Acids

128.55

Weight (kDa)

6.29

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 66 1.3e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 235 - 471 1e-09 Leucine-rich repeat region
LRR_8 PF13855 290 - 348 9.7e-08 Leucine rich repeat
LRR_14 PF23598 431 - 565 5.4e-06 Leucine-rich repeat region
LRR_8 PF13855 487 - 544 7.2e-07 Leucine rich repeat
Island PF20141 582 - 646 8.7e-32 Brassinosteroid receptor island domain
LRR_14 PF23598 655 - 730 8.9e-06 Leucine-rich repeat region
LRR_8 PF13855 675 - 733 2.4e-06 Leucine rich repeat
Pkinase PF00069 870 - 1139 1.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 873 - 1139 8.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39400
fragaria_vesca FvH4_1g23130
malus_domestica MD01G1014200.v1.1 MD15G1336800.v1.1
prunus_persica Prupe.6G168100_v2.0.a1
pyrus_communis pycom01g04600 pycom15g28930
rosa_chinensis RchiOBHm_Chr2g0117851
rosa_laevigata RLG00000018379
rosa_multiflora Rmu_co8475017.1_g000001 Rmu_sc0012910.1_g000002 Rmu_sc0022025.1_g000002
rosa_rugosa Rorug02G0214500
rosa_samantha Rh2AG270700 Rh2BG282200 Rh2CG268900 Rh2DG278500 Rh2DG296400
rosa_wichuraiana Rw2G021400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
AatII GACGTC 2 cut(s) 777, 3211
Acc65I GGTACC 1 cut(s) 1352
AccB1I GGYRCC 5 cut(s) 983, 1352, 2325, 2516, 3387
AccI GTMKAC 1 cut(s) 2652
AccII CGCG 2 cut(s) 779, 1392
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 7 cut(s) 468, 561, 1422, 1754, 1844, 2069, 3391
AclWI GGATC 7 cut(s) 581, 966, 1185, 1223, 1695, 2932, 3290
AcoI YGGCCR 2 cut(s) 501, 852
AcsI RAATTY 6 cut(s) 761, 1428, 1759, 1828, 2287, 2729
AcuI CTGAAG 4 cut(s) 66, 857, 1115, 1634
AcyI GRCGYC 4 cut(s) 96, 774, 3208, 3388
AfaI GTAC 9 cut(s) 910, 1132, 1256, 1282, 1354, 1714, 2018, 2497, 2834
AgeI ACCGGT 2 cut(s) 1052, 1349
AhlI ACTAGT 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 1031, 1063
AleI CACNNNNGTG 1 cut(s) 1904
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
Alw21I GWGCWC 6 cut(s) 233, 1007, 1187, 1443, 3092, 3306
Alw26I GTCTC 6 cut(s) 652, 662, 1029, 1110, 2225, 3037
AlwI GGATC 7 cut(s) 581, 966, 1185, 1223, 1695, 2932, 3290
AlwNI CAGNNNCTG 1 cut(s) 1305
Ama87I CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
Aor13HI TCCGGA 1 cut(s) 991
ApeKI GCWGC 7 cut(s) 360, 588, 591, 1089, 2300, 2676, 3193
ApoI RAATTY 6 cut(s) 761, 1428, 1759, 1828, 2287, 2729
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 1858, 1890
AsiGI ACCGGT 2 cut(s) 1052, 1349
Asp700I GAANNNNTTC 1 cut(s) 1193
Asp718I GGTACC 1 cut(s) 1352
AspLEI GCGC 5 cut(s) 781, 1013, 3365, 3390, 3418
AsuC2I CCSGG 7 cut(s) 208, 209, 1495, 1652, 3444, 3445, 3537
AsuNHI GCTAGC 1 cut(s) 1364
AvaI CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
AvaII GGWCC 6 cut(s) 526, 842, 1947, 2092, 3316, 3440
BaeGI GKGCMC 3 cut(s) 986, 2328, 2521
BanI GGYRCC 5 cut(s) 983, 1352, 2325, 2516, 3387
BanII GRGCYC 5 cut(s) 233, 1007, 1187, 1443, 3306
BarI GAAGNNNNNNTAC 4 cut(s) 1238, 1270, 3196, 3228
BauI CACGAG 1 cut(s) 1316
BbsI GAAGAC 2 cut(s) 3211, 3531
Bbv12I GWGCWC 6 cut(s) 233, 1007, 1187, 1443, 3092, 3306
BbvI GCAGC 7 cut(s) 372, 575, 603, 1101, 2287, 2688, 3180
BceAI ACGGC 5 cut(s) 202, 548, 553, 601, 2831
BcgI CGANNNNNNTGC 6 cut(s) 470, 504, 646, 680, 1297, 1331
BciVI GTATCC 2 cut(s) 589, 2786
BclI TGATCA 1 cut(s) 1398
BcnI CCSGG 7 cut(s) 208, 209, 1495, 1652, 3444, 3445, 3537
BcoDI GTCTC 6 cut(s) 652, 662, 1029, 1110, 2225, 3037
BcuI ACTAGT 1 cut(s) 607
BfaI CTAG 9 cut(s) 386, 608, 947, 1166, 1365, 1902, 2072, 2115, 3020
BfmI CTRYAG 2 cut(s) 589, 3213
BfoI RGCGCY 1 cut(s) 3391
BfuI GTATCC 2 cut(s) 589, 2786
BglI GCCNNNNNGGC 2 cut(s) 327, 2274
BglII AGATCT 2 cut(s) 382, 2584
BisI GCNGC 8 cut(s) 361, 589, 592, 1090, 2301, 2677, 3194, 3391
BlpI GCTNAGC 2 cut(s) 1085, 1418
BlsI GCNGC 8 cut(s) 362, 590, 593, 1091, 2302, 2678, 3195, 3392
Bme18I GGWCC 6 cut(s) 526, 842, 1947, 2092, 3316, 3440
BmeT110I CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
BmsI GCATC 2 cut(s) 985, 2406
BmtI GCTAGC 1 cut(s) 1368
BoxI GACNNNNGTC 1 cut(s) 2347
BpiI GAAGAC 2 cut(s) 3211, 3531
BplI GAGNNNNNCTC 4 cut(s) 1090, 1122, 1905, 1937
BpmI CTGGAG 7 cut(s) 506, 808, 1244, 1275, 1481, 2079, 3105
Bpu10I CCTNAGC 2 cut(s) 110, 227
Bpu1102I GCTNAGC 2 cut(s) 1085, 1418
BpuEI CTTGAG 3 cut(s) 599, 2612, 3347
BpuMI CCSGG 7 cut(s) 208, 209, 1495, 1652, 3444, 3445, 3537
Bsa29I ATCGAT 1 cut(s) 2178
BsaBI GATNNNNATC 3 cut(s) 2174, 2705, 3015
BsaHI GRCGYC 4 cut(s) 96, 774, 3208, 3388
BsaI GGTCTC 1 cut(s) 662
BsaWI WCCGGW 6 cut(s) 416, 839, 991, 1052, 1349, 2492
Bse118I RCCGGY 7 cut(s) 317, 326, 554, 632, 1052, 1199, 1349
Bse1I ACTGG 2 cut(s) 2224, 2943
Bse3DI GCAATG 4 cut(s) 901, 1749, 2376, 3072
Bse8I GATNNNNATC 3 cut(s) 2174, 2705, 3015
BseAI TCCGGA 1 cut(s) 991
BseCI ATCGAT 1 cut(s) 2178
BseGI GGATG 5 cut(s) 1000, 1356, 2677, 3379, 3453
BseJI GATNNNNATC 3 cut(s) 2174, 2705, 3015
BseMI GCAATG 4 cut(s) 901, 1749, 2376, 3072
BseNI ACTGG 2 cut(s) 2224, 2943
BseRI GAGGAG 5 cut(s) 32, 35, 359, 467, 673
BseSI GKGCMC 3 cut(s) 986, 2328, 2521
BseXI GCAGC 7 cut(s) 372, 575, 603, 1101, 2287, 2688, 3180
BseYI CCCAGC 5 cut(s) 99, 425, 860, 920, 1657
BsgI GTGCAG 5 cut(s) 379, 781, 1291, 1507, 3513
Bsh1236I CGCG 2 cut(s) 779, 1392
BshNI GGYRCC 5 cut(s) 983, 1352, 2325, 2516, 3387
BshTI ACCGGT 2 cut(s) 1052, 1349
BshVI ATCGAT 1 cut(s) 2178
BsiHKAI GWGCWC 6 cut(s) 233, 1007, 1187, 1443, 3092, 3306
BsiHKCI CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
BslFI GGGAC 6 cut(s) 828, 1510, 1676, 1960, 2335, 2996
BsmAI GTCTC 6 cut(s) 652, 662, 1029, 1110, 2225, 3037
BsmFI GGGAC 6 cut(s) 828, 1510, 1676, 1960, 2335, 2996
BsmI GAATGC 2 cut(s) 1992, 2961
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
Bsp1286I GDGCHC 9 cut(s) 233, 986, 1007, 1187, 1443, 2328, 2521, 3092, 3306
Bsp13I TCCGGA 1 cut(s) 991
Bsp1720I GCTNAGC 2 cut(s) 1085, 1418
Bsp19I CCATGG 2 cut(s) 1816, 3383
Bsp68I TCGCGA 1 cut(s) 1392
BspACI CCGC 7 cut(s) 468, 561, 1422, 1754, 1844, 2069, 3391
BspDI ATCGAT 1 cut(s) 2178
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 2 cut(s) 779, 1392
BspHI TCATGA 1 cut(s) 3306
BspMAI CTGCAG 1 cut(s) 593
BspOI GCTAGC 1 cut(s) 1368
BspPI GGATC 7 cut(s) 581, 966, 1185, 1223, 1695, 2932, 3290
BspT107I GGYRCC 5 cut(s) 983, 1352, 2325, 2516, 3387
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 4 cut(s) 901, 1749, 2376, 3072
BsrFI RCCGGY 7 cut(s) 317, 326, 554, 632, 1052, 1199, 1349
BsrI ACTGG 2 cut(s) 2224, 2943
BssAI RCCGGY 7 cut(s) 317, 326, 554, 632, 1052, 1199, 1349
BssNAI GTATAC 1 cut(s) 2653
BssNI GRCGYC 4 cut(s) 96, 774, 3208, 3388
BssSI CACGAG 1 cut(s) 1316
BssT1I CCWWGG 5 cut(s) 492, 1017, 1816, 2788, 3383
Bst1107I GTATAC 1 cut(s) 2653
Bst2BI CACGAG 1 cut(s) 1316
Bst6I CTCTTC 6 cut(s) 53, 76, 85, 132, 796, 1091
BstACI GRCGYC 4 cut(s) 96, 774, 3208, 3388
BstAPI GCANNNNNTGC 1 cut(s) 1812
BstDSI CCRYGG 3 cut(s) 569, 1816, 3383
BstF5I GGATG 5 cut(s) 1000, 1356, 2677, 3379, 3453
BstFNI CGCG 2 cut(s) 779, 1392
BstH2I RGCGCY 1 cut(s) 3391
BstHHI GCGC 5 cut(s) 781, 1013, 3365, 3390, 3418
BstMAI GTCTC 6 cut(s) 652, 662, 1029, 1110, 2225, 3037
BstNSI RCATGY 2 cut(s) 785, 3269
BstPAI GACNNNNGTC 1 cut(s) 2347
BstSFI CTRYAG 2 cut(s) 589, 3213
BstSLI GKGCMC 3 cut(s) 986, 2328, 2521
BstUI CGCG 2 cut(s) 779, 1392
BstV1I GCAGC 7 cut(s) 372, 575, 603, 1101, 2287, 2688, 3180
BstV2I GAAGAC 2 cut(s) 3211, 3531
BstX2I RGATCY 7 cut(s) 382, 958, 1177, 1228, 1687, 2584, 2924
BstXI CCANNNNNNTGG 1 cut(s) 2170
BstYI RGATCY 7 cut(s) 382, 958, 1177, 1228, 1687, 2584, 2924
BstZ17I GTATAC 1 cut(s) 2653
Bsu15I ATCGAT 1 cut(s) 2178
BsuI GTATCC 2 cut(s) 589, 2786
BsuTUI ATCGAT 1 cut(s) 2178
BtgI CCRYGG 3 cut(s) 569, 1816, 3383
BtgZI GCGATG 1 cut(s) 3174
BtsCI GGATG 5 cut(s) 1000, 1356, 2677, 3379, 3453
BtsI GCAGTG 2 cut(s) 2368, 2733
BtsIMutI CAGTG 3 cut(s) 2368, 2716, 2733
BtuMI TCGCGA 1 cut(s) 1392
CaiI CAGNNNCTG 1 cut(s) 1305
CciI TCATGA 1 cut(s) 3306
CfoI GCGC 5 cut(s) 781, 1013, 3365, 3390, 3418
Cfr10I RCCGGY 7 cut(s) 317, 326, 554, 632, 1052, 1199, 1349
Cfr9I CCCGGG 2 cut(s) 207, 3443
ClaI ATCGAT 1 cut(s) 2178
CseI GACGC 2 cut(s) 104, 2337
CsiI ACCWGGT 1 cut(s) 3106
Csp6I GTAC 9 cut(s) 909, 1131, 1255, 1281, 1353, 1713, 2017, 2496, 2833
CspAI ACCGGT 2 cut(s) 1052, 1349
CspCI CAANNNNNGTGG 4 cut(s) 906, 941, 1546, 1581
CviQI GTAC 9 cut(s) 909, 1131, 1255, 1281, 1353, 1713, 2017, 2496, 2833
DinI GGCGCC 1 cut(s) 3389
DraI TTTAAA 1 cut(s) 2286
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 2 cut(s) 501, 852
Eam1104I CTCTTC 6 cut(s) 53, 76, 85, 132, 796, 1091
EarI CTCTTC 6 cut(s) 53, 76, 85, 132, 796, 1091
EciI GGCGGA 1 cut(s) 457
Ecl136II GAGCTC 5 cut(s) 231, 1005, 1185, 1441, 3304
Eco130I CCWWGG 5 cut(s) 492, 1017, 1816, 2788, 3383
Eco147I AGGCCT 1 cut(s) 2268
Eco24I GRGCYC 5 cut(s) 233, 1007, 1187, 1443, 3306
Eco31I GGTCTC 1 cut(s) 662
Eco47I GGWCC 6 cut(s) 526, 842, 1947, 2092, 3316, 3440
Eco53kI GAGCTC 5 cut(s) 231, 1005, 1185, 1441, 3304
Eco57I CTGAAG 4 cut(s) 66, 857, 1115, 1634
Eco88I CYCGRG 9 cut(s) 207, 442, 479, 665, 979, 1835, 2520, 3188, 3443
EcoICRI GAGCTC 5 cut(s) 231, 1005, 1185, 1441, 3304
EcoO109I RGGNCCY 4 cut(s) 526, 2092, 3316, 3440
EcoRI GAATTC 1 cut(s) 2729
EcoT14I CCWWGG 5 cut(s) 492, 1017, 1816, 2788, 3383
EcoT22I ATGCAT 1 cut(s) 1000
EcoT38I GRGCYC 5 cut(s) 233, 1007, 1187, 1443, 3306
EgeI GGCGCC 1 cut(s) 3389
EheI GGCGCC 1 cut(s) 3389
ErhI CCWWGG 5 cut(s) 492, 1017, 1816, 2788, 3383
FalI AAGNNNNNCTT 2 cut(s) 2802, 2834
FaqI GGGAC 6 cut(s) 828, 1510, 1676, 1960, 2335, 2996
FauI CCCGC 4 cut(s) 568, 1415, 1837, 2076
FbaI TGATCA 1 cut(s) 1398
FblI GTMKAC 1 cut(s) 2652
Fnu4HI GCNGC 8 cut(s) 361, 589, 592, 1090, 2301, 2677, 3194, 3391
FokI GGATG 5 cut(s) 1007, 1343, 2684, 3386, 3460
FriOI GRGCYC 5 cut(s) 233, 1007, 1187, 1443, 3306
Fsp4HI GCNGC 8 cut(s) 361, 589, 592, 1090, 2301, 2677, 3194, 3391
FspBI CTAG 9 cut(s) 386, 608, 947, 1166, 1365, 1902, 2072, 2115, 3020
GlaI GCGC 5 cut(s) 780, 1012, 3364, 3389, 3417
GluI GCNGC 8 cut(s) 361, 589, 592, 1090, 2301, 2677, 3194, 3391
GsaI CCCAGC 5 cut(s) 103, 429, 864, 924, 1661
GsuI CTGGAG 7 cut(s) 506, 808, 1244, 1275, 1481, 2079, 3105
HaeII RGCGCY 1 cut(s) 3391
HgaI GACGC 2 cut(s) 104, 2337
HhaI GCGC 5 cut(s) 781, 1013, 3365, 3390, 3418
Hin1I GRCGYC 4 cut(s) 96, 774, 3208, 3388
Hin6I GCGC 5 cut(s) 779, 1011, 3363, 3388, 3416
HinP1I GCGC 5 cut(s) 779, 1011, 3363, 3388, 3416
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 3 cut(s) 1385, 1592, 2848
Hpy166II GTNNAC 6 cut(s) 238, 246, 2476, 2653, 2714, 2827
Hpy8I GTNNAC 6 cut(s) 238, 246, 2476, 2653, 2714, 2827
Hpy99I CGWCG 4 cut(s) 587, 776, 779, 3479
HpyAV CCTTC 5 cut(s) 550, 1240, 2083, 2443, 2662
HpyCH4IV ACGT 7 cut(s) 506, 774, 809, 1778, 2466, 3000, 3208
HpySE526I ACGT 7 cut(s) 506, 774, 809, 1778, 2466, 3000, 3208
Hsp92I GRCGYC 4 cut(s) 96, 774, 3208, 3388
HspAI GCGC 5 cut(s) 779, 1011, 3363, 3388, 3416
KasI GGCGCC 1 cut(s) 3387
Kpn2I TCCGGA 1 cut(s) 991
KpnI GGTACC 1 cut(s) 1356
KroI GCCGGC 1 cut(s) 554
KroNI GCCGGC 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 1398
Lsp1109I GCAGC 7 cut(s) 372, 575, 603, 1101, 2287, 2688, 3180
LweI GCATC 2 cut(s) 985, 2406
MabI ACCWGGT 1 cut(s) 3106
MaeI CTAG 9 cut(s) 386, 608, 947, 1166, 1365, 1902, 2072, 2115, 3020
MaeII ACGT 7 cut(s) 506, 774, 809, 1778, 2466, 3000, 3208
MaeIII GTNAC 5 cut(s) 1517, 1622, 1968, 3233, 3339
MfeI CAATTG 4 cut(s) 1862, 2421, 2663, 2956
MflI RGATCY 7 cut(s) 382, 958, 1177, 1228, 1687, 2584, 2924
MhlI GDGCHC 9 cut(s) 233, 986, 1007, 1187, 1443, 2328, 2521, 3092, 3306
Mly113I GGCGCC 1 cut(s) 3388
MlyI GAGTC 6 cut(s) 491, 507, 950, 1031, 3039, 3446
MmeI TCCRAC 5 cut(s) 606, 711, 1062, 1665, 1948
Mph1103I ATGCAT 1 cut(s) 1000
MroI TCCGGA 1 cut(s) 991
MroNI GCCGGC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 1193
MseI TTAA 8 cut(s) 459, 1935, 2097, 2285, 2531, 2538, 3275, 3350
MslI CAYNNNNRTG 5 cut(s) 1754, 1904, 2168, 2709, 3081
MspA1I CMGCKG 2 cut(s) 1242, 3224
MunI CAATTG 4 cut(s) 1862, 2421, 2663, 2956
Mva1269I GAATGC 2 cut(s) 1992, 2961
MvnI CGCG 2 cut(s) 779, 1392
NaeI GCCGGC 1 cut(s) 556
NarI GGCGCC 1 cut(s) 3388
NciI CCSGG 7 cut(s) 208, 209, 1495, 1652, 3444, 3445, 3537
NcoI CCATGG 2 cut(s) 1816, 3383
NgoMIV GCCGGC 1 cut(s) 554
NheI GCTAGC 1 cut(s) 1364
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 1968, 3233
NruI TCGCGA 1 cut(s) 1392
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 2 cut(s) 785, 3269
OliI CACNNNNGTG 1 cut(s) 1904
PaeI GCATGC 1 cut(s) 785
PaeR7I CTCGAG 5 cut(s) 442, 479, 665, 1835, 3188
PagI TCATGA 1 cut(s) 3306
PceI AGGCCT 1 cut(s) 2268
PcsI WCGNNNNNNNCGW 4 cut(s) 591, 672, 806, 3006
PctI GAATGC 2 cut(s) 1992, 2961
PdiI GCCGGC 1 cut(s) 556
PdmI GAANNNNTTC 1 cut(s) 1193
PfoI TCCNGGA 1 cut(s) 1473
PinAI ACCGGT 2 cut(s) 1052, 1349
PkrI GCNGC 8 cut(s) 362, 590, 593, 1091, 2302, 2678, 3195, 3392
PleI GAGTC 6 cut(s) 491, 507, 949, 1030, 3038, 3446
PluTI GGCGCC 1 cut(s) 3391
PpsI GAGTC 6 cut(s) 491, 507, 949, 1030, 3038, 3446
PpuMI RGGWCCY 4 cut(s) 526, 2092, 3316, 3440
PshAI GACNNNNGTC 1 cut(s) 2347
Psp124BI GAGCTC 5 cut(s) 233, 1007, 1187, 1443, 3306
Psp5II RGGWCCY 4 cut(s) 526, 2092, 3316, 3440
PspFI CCCAGC 5 cut(s) 99, 425, 860, 920, 1657
PspPPI RGGWCCY 4 cut(s) 526, 2092, 3316, 3440
PspXI VCTCGAGB 4 cut(s) 442, 479, 1835, 3188
PsrI GAACNNNNNNTAC 2 cut(s) 594, 626
PstI CTGCAG 1 cut(s) 593
PstNI CAGNNNCTG 1 cut(s) 1305
PsuI RGATCY 7 cut(s) 382, 958, 1177, 1228, 1687, 2584, 2924
PvuII CAGCTG 2 cut(s) 1242, 3224
RruI TCGCGA 1 cut(s) 1392
RsaI GTAC 9 cut(s) 910, 1132, 1256, 1282, 1354, 1714, 2018, 2497, 2834
RsaNI GTAC 9 cut(s) 909, 1131, 1255, 1281, 1353, 1713, 2017, 2496, 2833
RseI CAYNNNNRTG 5 cut(s) 1754, 1904, 2168, 2709, 3081
SacI GAGCTC 5 cut(s) 233, 1007, 1187, 1443, 3306
SaqAI TTAA 8 cut(s) 459, 1935, 2097, 2285, 2531, 2538, 3275, 3350
SatI GCNGC 8 cut(s) 361, 589, 592, 1090, 2301, 2677, 3194, 3391
SchI GAGTC 6 cut(s) 491, 507, 950, 1031, 3039, 3446
SduI GDGCHC 9 cut(s) 233, 986, 1007, 1187, 1443, 2328, 2521, 3092, 3306
SexAI ACCWGGT 1 cut(s) 3106
SfaNI GCATC 2 cut(s) 985, 2406
SfcI CTRYAG 2 cut(s) 589, 3213
SfiI GGCCNNNNNGGCC 1 cut(s) 327
SfoI GGCGCC 1 cut(s) 3389
Sfr274I CTCGAG 5 cut(s) 442, 479, 665, 1835, 3188
SgrAI CRCCGGYG 2 cut(s) 632, 1052
SinI GGWCC 6 cut(s) 526, 842, 1947, 2092, 3316, 3440
SlaI CTCGAG 5 cut(s) 442, 479, 665, 1835, 3188
SmaI CCCGGG 2 cut(s) 209, 3445
SmiMI CAYNNNNRTG 5 cut(s) 1754, 1904, 2168, 2709, 3081
SmlI CTYRAG 8 cut(s) 442, 479, 614, 665, 1835, 2591, 3188, 3326
SmoI CTYRAG 8 cut(s) 442, 479, 614, 665, 1835, 2591, 3188, 3326
SpeI ACTAGT 1 cut(s) 607
SphI GCATGC 1 cut(s) 785
SseBI AGGCCT 1 cut(s) 2268
SsiI CCGC 7 cut(s) 468, 561, 1422, 1754, 1844, 2069, 3391
SspDI GGCGCC 1 cut(s) 3387
SspMI CTAG 9 cut(s) 386, 608, 947, 1166, 1365, 1902, 2072, 2115, 3020
SstI GAGCTC 5 cut(s) 233, 1007, 1187, 1443, 3306
StuI AGGCCT 1 cut(s) 2268
StyI CCWWGG 5 cut(s) 492, 1017, 1816, 2788, 3383
TaiI ACGT 7 cut(s) 509, 777, 812, 1781, 2469, 3003, 3211
TatI WGTACW 2 cut(s) 2016, 2832
TauI GCSGC 1 cut(s) 3393
Tru1I TTAA 8 cut(s) 459, 1935, 2097, 2285, 2531, 2538, 3275, 3350
Tru9I TTAA 8 cut(s) 459, 1935, 2097, 2285, 2531, 2538, 3275, 3350
TscAI CASTG 3 cut(s) 2368, 2716, 2740
TseFI GTSAC 2 cut(s) 1968, 3233
TseI GCWGC 7 cut(s) 360, 588, 591, 1089, 2300, 2676, 3193
Tsp45I GTSAC 2 cut(s) 1968, 3233
TspDTI ATGAA 7 cut(s) 17, 1149, 2270, 2852, 2966, 3002, 3323
TspGWI ACGGA 2 cut(s) 223, 699
TspMI CCCGGG 2 cut(s) 207, 3443
TspRI CASTG 3 cut(s) 2368, 2716, 2740
VpaK11BI GGWCC 6 cut(s) 526, 842, 1947, 2092, 3316, 3440
XapI RAATTY 6 cut(s) 761, 1428, 1759, 1828, 2287, 2729
XbaI TCTAGA 2 cut(s) 1165, 3019
XceI RCATGY 2 cut(s) 785, 3269
XcmI CCANNNNNNNNNTGG 1 cut(s) 2669
XhoI CTCGAG 5 cut(s) 442, 479, 665, 1835, 3188
XmaI CCCGGG 2 cut(s) 207, 3443
XmiI GTMKAC 1 cut(s) 2652
XmnI GAANNNNTTC 1 cut(s) 1193
XspI CTAG 9 cut(s) 386, 608, 947, 1166, 1365, 1902, 2072, 2115, 3020
ZraI GACGTC 2 cut(s) 775, 3209
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.