FvH4_1g23450

trigalactosyldiacylglycerol 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007397
Physical Location & Seq
Forward (+)
15306796 .. 15311603
4808 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23450.t2

Sequence Viewer

Length: 1134 bp
ATGATAGGGAATTCTTTGGTCCAAGTCTCAACATGCCCCACAATGTTGTCCTTGTCACGTTTTTCTTCAAATGGCTTGCCTTATCTTCCTCAACAGTCAACAAAACTAACCCGGATAAGGGCTACATCTGCTAATACTGGACACAGTCAACCACCCTCCTCTTCCAAGAGAGAAAGGAATATACTTGCAGTTGTTTTAGATGCTCCTCGTACTATTTGGAGGCAAACATTACGACCATTGAGTGATTTTGGGTTTGGTCATAGAAGCATATGGGAAGGCGGGGTAGGGCTGTTTCTAGTATCAGGGGCAGTTCTCCTTGCACTCAGTTTGGCTTGGTTGAGGGGTTATCAATTGCGTTCTAGATTCAGGAAGTACTTCGCTGTGTTTGAGTTTACTCAAGCTTGTGGAATAGCAACCGGAACCCCAGTGCGGATCAGAGGGGTCACTGTTGGAAATGTTATCCGTGTCAACTCGTCCTTGCAAAGTATCGAAGCTGTTGTTGAGGTTGAAGATGATAAAACTGTCATACCTCGAAATTCACTGATTGAGGTAAATCAGTCAGGTCTTCTCATGGAAACGAGAATTGACATTACACCAAGAGATCCAATTCCTAATGTTTCAGTTGGGCCTCTTCATCCTGAATGTGACAAAGAGGGTGTAATTGTATGTGATAGGCAAAAGATGAAGGGAAATCAAGGGGTAAGTTTAGATGCATTGGTAGGCATATTCACCCGTCTGGGACGTGAAGTGGAGGAGATTGGTATTGTGAACACCTATTCACTTGCTGAGCGTGTTGCAGCTGTTATAGAAGAGGCAAAGCCACTGCTGAAAAAGATCGAAGCCATGGCTGAAGATGTGCAACCTTTGTTGGCTGAGATTCATGATAGTGGTCTGCTAACGGAAGTTGAGACTTTGACCAAAAGCCTTACGCAAGCCTCGGAAGATTTGAGGAGAGTGCACTCATCTATTATGACCCCTGAGAACACGGAACTGATTCAGAAGTCCATATACACTCTGATTTTCACCATGAAGAACATTGAGAACATTAGCTCTGATATTTTGGGATTCACGGGTGATGAGGCTACAAGAAAAAATTTGAAGTTACTCATCAAGTCTCTCAGCAGGCTAGTATGA

Protein Analysis

378

Amino Acids

41.55

Weight (kDa)

8.62

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaD PF02470 124 - 199 1.2e-15 MlaD protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 279, 430
AclWI GGATC 2 cut(s) 440, 596
AcsI RAATTY 3 cut(s) 10, 535, 1093
AcuI CTGAAG 1 cut(s) 870
AfaI GTAC 2 cut(s) 211, 374
AfiI CCNNNNNNNGG 3 cut(s) 117, 118, 429
AgsI TTSAA 3 cut(s) 69, 509, 1099
AjiI CACGTC 1 cut(s) 743
AluBI AGCT 4 cut(s) 401, 494, 800, 1050
AluI AGCT 4 cut(s) 401, 494, 800, 1050
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 3 cut(s) 31, 902, 1119
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 2 cut(s) 440, 596
AoxI GGCC 1 cut(s) 626
ApaLI GTGCAC 1 cut(s) 956
ApeKI GCWGC 1 cut(s) 797
ApoI RAATTY 3 cut(s) 10, 535, 1093
Asp700I GAANNNNTTC 2 cut(s) 374, 993
AspS9I GGNCC 2 cut(s) 19, 626
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 721, 1015, 1085
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 960
BarI GAAGNNNNNNTAC 2 cut(s) 992, 1024
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 960
BbvI GCAGC 1 cut(s) 809
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 3 cut(s) 31, 902, 1119
BfaI CTAG 3 cut(s) 296, 360, 1127
BisI GCNGC 1 cut(s) 798
BlpI GCTNAGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 799
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 19
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 2 cut(s) 19, 626
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 2 cut(s) 190, 700
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 557
Bpu1102I GCTNAGC 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 843, 936
BsaWI WCCGGW 1 cut(s) 416
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 118, 429
Bse1I ACTGG 2 cut(s) 142, 425
BseDI CCNNGG 2 cut(s) 843, 936
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 3 cut(s) 117, 118, 429
BseMII CTCAG 5 cut(s) 337, 777, 864, 969, 1132
BseNI ACTGG 2 cut(s) 142, 425
BseRI GAGGAG 4 cut(s) 148, 195, 767, 964
BseSI GKGCMC 1 cut(s) 960
BseXI GCAGC 1 cut(s) 809
BshFI GGCC 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 960
BsiSI CCGG 2 cut(s) 112, 417
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 3 cut(s) 117, 118, 429
BsmAI GTCTC 3 cut(s) 31, 902, 1119
BsmFI GGGAC 1 cut(s) 753
BsnI GGCC 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 960
Bsp143I GATC 3 cut(s) 432, 601, 834
Bsp1720I GCTNAGC 1 cut(s) 786
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 2 cut(s) 279, 430
BspANI GGCC 1 cut(s) 628
BspCNI CTCAG 5 cut(s) 336, 778, 865, 970, 1131
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 1 cut(s) 421
BspPI GGATC 2 cut(s) 440, 596
BsrI ACTGG 2 cut(s) 142, 425
BssECI CCNNGG 2 cut(s) 843, 936
BssMI GATC 3 cut(s) 432, 601, 834
BssT1I CCWWGG 1 cut(s) 843
Bst4CI ACNGT 4 cut(s) 96, 146, 448, 523
Bst6I CTCTTC 3 cut(s) 166, 636, 804
BstC8I GCNNGC 3 cut(s) 77, 933, 1124
BstDEI CTNAG 5 cut(s) 323, 786, 873, 978, 1118
BstDSI CCRYGG 1 cut(s) 843
BstF5I GGATG 1 cut(s) 634
BstKTI GATC 3 cut(s) 435, 604, 837
BstMAI GTCTC 3 cut(s) 31, 902, 1119
BstMBI GATC 3 cut(s) 432, 601, 834
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 809
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 1 cut(s) 628
BtgI CCRYGG 1 cut(s) 843
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 4 cut(s) 432, 444, 539, 821
Cac8I GCNNGC 3 cut(s) 77, 933, 1124
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 2 cut(s) 19, 626
Csp6I GTAC 2 cut(s) 210, 373
CviAII CATG 5 cut(s) 33, 571, 844, 881, 1027
CviQI GTAC 2 cut(s) 210, 373
DdeI CTNAG 5 cut(s) 323, 786, 873, 978, 1118
DpnI GATC 3 cut(s) 434, 603, 836
DpnII GATC 3 cut(s) 432, 601, 834
Eam1104I CTCTTC 3 cut(s) 166, 636, 804
EarI CTCTTC 3 cut(s) 166, 636, 804
Eco130I CCWWGG 1 cut(s) 843
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 870
EcoRI GAATTC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 843
EcoT22I ATGCAT 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 843
FaeI CATG 5 cut(s) 36, 574, 847, 884, 1030
FaqI GGGAC 1 cut(s) 753
FatI CATG 5 cut(s) 32, 570, 843, 880, 1026
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 1 cut(s) 798
FokI GGATG 1 cut(s) 621
Fsp4HI GCNGC 1 cut(s) 798
FspBI CTAG 3 cut(s) 296, 360, 1127
GluI GCNGC 1 cut(s) 798
HaeIII GGCC 1 cut(s) 628
HapII CCGG 2 cut(s) 112, 417
Hin1II CATG 5 cut(s) 36, 574, 847, 884, 1030
HincII GTYRAC 3 cut(s) 99, 149, 469
HindII GTYRAC 3 cut(s) 99, 149, 469
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 4 cut(s) 363, 877, 994, 1065
HpaII CCGG 2 cut(s) 112, 417
HphI GGTGA 3 cut(s) 721, 1015, 1085
Hpy166II GTNNAC 6 cut(s) 99, 149, 393, 469, 769, 958
Hpy188I TCNGA 5 cut(s) 437, 940, 999, 1017, 1054
Hpy188III TCNNGA 4 cut(s) 360, 367, 638, 881
Hpy8I GTNNAC 6 cut(s) 99, 149, 393, 469, 769, 958
HpyAV CCTTC 2 cut(s) 269, 679
HpyCH4III ACNGT 4 cut(s) 96, 146, 448, 523
HpyCH4IV ACGT 2 cut(s) 58, 742
HpyCH4V TGCA 7 cut(s) 188, 320, 481, 713, 797, 859, 958
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 5 cut(s) 323, 786, 873, 978, 1118
HpySE526I ACGT 2 cut(s) 58, 742
Hsp92II CATG 5 cut(s) 36, 574, 847, 884, 1030
Kzo9I GATC 3 cut(s) 432, 601, 834
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 1 cut(s) 809
LweI GCATC 2 cut(s) 190, 700
MaeI CTAG 3 cut(s) 296, 360, 1127
MaeII ACGT 2 cut(s) 58, 742
MaeIII GTNAC 4 cut(s) 54, 442, 644, 1101
MalI GATC 3 cut(s) 434, 603, 836
MboI GATC 3 cut(s) 432, 601, 834
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 960
MluCI AATT 7 cut(s) 10, 350, 535, 582, 606, 660, 1093
MmeI TCCRAC 1 cut(s) 430
Mph1103I ATGCAT 1 cut(s) 715
MroXI GAANNNNTTC 2 cut(s) 374, 993
MslI CAYNNNNRTG 1 cut(s) 885
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 2 cut(s) 112, 417
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 350
MwoI GCNNNNNNNGC 1 cut(s) 128
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 432, 601, 834
NlaIII CATG 5 cut(s) 36, 574, 847, 884, 1030
NlaIV GGNNCC 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 54, 442, 644
NsiI ATGCAT 1 cut(s) 715
NspI RCATGY 1 cut(s) 36
PagI TCATGA 1 cut(s) 880
PdmI GAANNNNTTC 2 cut(s) 374, 993
PfeI GAWTC 4 cut(s) 363, 877, 994, 1065
PflFI GACNNNGTC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 2 cut(s) 19, 626
PsuI RGATCY 1 cut(s) 601
PsyI GACNNNGTC 1 cut(s) 144
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 2 cut(s) 211, 374
RsaNI GTAC 2 cut(s) 210, 373
RseI CAYNNNNRTG 1 cut(s) 885
SatI GCNGC 1 cut(s) 798
Sau3AI GATC 3 cut(s) 432, 601, 834
Sau96I GGNCC 2 cut(s) 19, 626
ScaI AGTACT 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 1 cut(s) 960
SfaNI GCATC 2 cut(s) 190, 700
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 7 cut(s) 10, 350, 535, 582, 606, 660, 1093
SsiI CCGC 2 cut(s) 279, 430
SspMI CTAG 3 cut(s) 296, 360, 1127
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 4 cut(s) 96, 146, 448, 523
TaiI ACGT 2 cut(s) 61, 745
TaqI TCGA 3 cut(s) 489, 532, 837
TasI AATT 7 cut(s) 10, 350, 535, 582, 606, 660, 1093
TatI WGTACW 1 cut(s) 372
TfiI GAWTC 4 cut(s) 363, 877, 994, 1065
TscAI CASTG 4 cut(s) 432, 451, 546, 828
TseFI GTSAC 3 cut(s) 54, 442, 644
TseI GCWGC 1 cut(s) 797
Tsp45I GTSAC 3 cut(s) 54, 442, 644
TspDTI ATGAA 4 cut(s) 623, 698, 869, 1043
TspGWI ACGGA 3 cut(s) 452, 914, 1001
TspRI CASTG 4 cut(s) 432, 451, 546, 828
Tth111I GACNNNGTC 1 cut(s) 144
VneI GTGCAC 1 cut(s) 956
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 3 cut(s) 10, 535, 1093
XbaI TCTAGA 1 cut(s) 359
XceI RCATGY 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 374, 993
XspI CTAG 3 cut(s) 296, 360, 1127
ZrmI AGTACT 1 cut(s) 374
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.